LOCUS       NZ_LNBE01000004        10399 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_4, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000004
VERSION     NZ_LNBE01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3642602)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 3642602)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   3  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   6  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 299497
            Orig. end                         :: 309896
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(335..1549)
                     /locus_tag="AS857_RS37840"
     CDS             complement(335..1549)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355277.1"
                     /locus_tag="AS857_RS37840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="WP_245700610.1"
                     /transl_table=11
                     /translation="MQGGYAPARGDREIGDLAGVAEDVLGDGPYEVDVEAAELAVGGDG
                     GVSDAGHGGCGGGGRGGGPPPPPAPPARPPPHRAEPGTGSPRSTRPGSAGTRRGGHAGS
                     RSGGTRPRRAEARARTRAAAPAAPAPDAAAPAQCTDPEASLRPAGGADGAAVQRIKARG
                     RLIAGVDQNSYRWGYRNPADGELSGFDIDLVRAVAKDILGDPGKVTYLAIPTSRRISAL
                     QTGKVDIVARTMTITCERIGQVAFSTAYFESGQQVMVPKGSPVTAFDDSLKGKKVCTAE
                     GSTAEIKVREEPHGSSLLTVPNQLDCLVRLQLGEVDAVVNDRALAAGQAAQDPSVQLVG
                     KPFTTEPYGVAMNLADTDLVRRVNKVLEDYRAGGAGSLWMKAYDKWLAADLKGITAPPA
                     PKYRD"
     gene            <1430..>1684
                     /locus_tag="AS857_RS37845"
                     /pseudo=""
     CDS             1430..>1684
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010063028.1"
                     /locus_tag="AS857_RS37845"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MELSGFDIDLVRAVAKDILGDPGKVTYLAIPTSRRISALQTGKVD
                     IVARTMTITCERIGQVAFSTAYFESGQQVMVPKGSPVTPW"
     misc_feature    1563..1583
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.09"
                     /tool="antismash"
     gene            complement(<1659..2198)
                     /locus_tag="AS857_RS18450"
                     /pseudo=""
     CDS             complement(<1659..2198)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762073.1"
                     /locus_tag="AS857_RS18450"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPSSAWAAGCRSTGETGTGGRGGGGAHGRGPDGAHAGSGAPVPKA
                     PPAQCTDPEASLRPAGGADGAAVQRIKARGRLIAGVDQNSYRWGYRNPADGELSGFDID
                     LVRAVAKDILGDPGKVTYLAIPTSRRISALQTGKVDIVARTMTITCERIGQVAFSTAYF
                     ESGQQVMVPKGSPVTPW"
     gene            complement(2491..3795)
                     /locus_tag="AS857_RS18455"
     CDS             complement(2491..3795)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944859.1"
                     /locus_tag="AS857_RS18455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058046888.1"
                     /transl_table=11
                     /translation="MRAAAVTEPGRLRVIGAVLAVVLVAFGAVTAWQVASRSAAADSVV
                     RTSQPLSADAADIYRSLADADTTAASGFLAGGQEPRAVRQRYEKDIALASRLLVKAAAN
                     AEGSATARARIATLNEGLPRYTGLVEAARANNRQGLPLGGAYLRYAGDRMRGELLPAAR
                     GLYEAETGRLGSDYDDAKSWPWAALALGAVALAALAWAQRRNYLRTNRVFNHGLLAATA
                     AAGVVLLWLVAGHAVARSGLETSYAHGAKSLQALNEARIGSLQARGDENLAMVARGAVL
                     GKNGDDAYETGYRDGMGRLVGGSQGPARPDTLLERARALADDAEGRDPVEAAVKNVREW
                     QQRHGQARALADDRGDYDDALRKVIGDKDSTGASFDRVDTELQRAIAHEQAEFRQEADD
                     GRGAFSGLAAGAAVLTVLGAAGAVVGVGRRLSEYR"
     gene            complement(4118..4939)
                     /locus_tag="AS857_RS18460"
     CDS             complement(4118..4939)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091617.1"
                     /locus_tag="AS857_RS18460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_058044412.1"
                     /transl_table=11
                     /translation="MAVRKNQANLTATEKRNFVNAVLELKRTGVYDGFVRTHNEFIMSD
                     TDNGDRVGHRSPSFLPWHRRFLIQFEQELQKIDSSVTLPYWDWTVDRTANASIFGADFM
                     GGNGRSRDAQVTTGPFAYSTGKWTINVSVDNRNFLQREIGAAGTLPTRAQVDAVLAMPV
                     YDTAPWNSASEGSFRNYLEGWRGAGRVHNNVHVWMGGQMATGVSPNDPVFWLHHAHIDK
                     LWSDWQRMHPQSAYLPAAGTTNVVDLNDTMRPWNNVTPAQMLDHSSYYTYA"
     gene            complement(5001..5399)
                     /locus_tag="AS857_RS18465"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038019.1"
                     /locus_tag="AS857_RS18465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase cofactor"
                     /protein_id="WP_058044413.1"
                     /sec_met_domain="melC (E-value: 7.9e-43, bitscore: 143.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSNVSRRVVLRGTAAAAAGVALLATGAQAADHTGHTTDHTGHGG
                     HGTGPAAFDEVYKGRRIVGKPVTGGHEAHHGGFAVTVDGKELHVMQNADGSWISVVNHY
                     QVFDQPLALTRAAVDALRGATLVPIAMN"
     misc_feature    5507..5521
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.39"
                     /tool="antismash"
     misc_feature    complement(5623..5637)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.94"
                     /tool="antismash"
     misc_feature    5718..5733
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     misc_feature    5734..5751
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.23"
                     /tool="antismash"
     misc_feature    complement(5736..5751)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.35"
                     /tool="antismash"
     misc_feature    complement(5737..5751)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.14"
                     /tool="antismash"
     gene            complement(5922..6914)
                     /locus_tag="AS857_RS18470"
     CDS             complement(5922..6914)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106032.1"
                     /locus_tag="AS857_RS18470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="WP_058044414.1"
                     /transl_table=11
                     /translation="MGVSGALLAVDAGNSKTDVVLAGPDGAVLGASRGGGFEPARTGVR
                     QAVDGLAALVERAAAAAGAAAPPYAAHVSACLANCDLPVEEELLTAEIAARGWGRTVAV
                     ANDTFALLRAGLPDGGEPVGVAVVCGAGINCAGLGPGGRTARFPAIGRLSGDWGGGACL
                     AEEALWAAARAADGRGGPTALAGALPAHFGLDGMPALIEALHLGRVAAERRHELVPVLF
                     AVAAAGDAVARALVTRQAEEVVAMAAVALERLGLLGAAVPVVLGGGVLAARHPLLHEQV
                     LALLASRAPQAVPLVVTAPPVLGAALLALDRAGAPATAHARLRAHYGDA"
     gene            complement(6905..8158)
                     /locus_tag="AS857_RS18475"
     CDS             complement(6905..8158)
                     /EC_number="3.2.1.86"
                     /GO_function="GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179641.1"
                     /locus_tag="AS857_RS18475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="WP_058044415.1"
                     /transl_table=11
                     /translation="MKLAVVGGGSTYTPELVDGFARLHDTLPVTELVLIDPDEERLELV
                     GALARRILARQGHPGRVTWTADLDAGVDGADAVLLQLRVGGQAARQQDETWPLECGCVG
                     QETTGAGGLAKALRTVPVVLDIAERVRRRSPDAWIVDFTNPVGIVTRALLAAGHRAVGL
                     CNVAIGFQRKFARLLGVAPEEVALEHVGLNHLTWERSVTVGGEDVLPRLIAEHGDAVAA
                     DLRMPRSVLDRLGVVPSYYLRYFYGHDEVVRELRTKPSRAAEVAAMERKLLSLYADPAL
                     DEKPALLAKRGGAFYSEAAVALTASLLRDTGDVQVVNALNDGTLPFLPDDAVIEVPAAV
                     DARGAHPLPVRPLEPLYAGLVANVTAYEHLALEAALEGGRDRVFRALLAHPLVGQFDHA
                     DRLTDALVAHNREHLAWA"
     gene            complement(8224..9405)
                     /locus_tag="AS857_RS18480"
     CDS             complement(8224..9405)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1108: ROK family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156247.1"
                     /locus_tag="AS857_RS18480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ROK family transcriptional regulator"
                     /protein_id="WP_058044416.1"
                     /transl_table=11
                     /translation="MAGTPGTPRVLRAMNDRAALELLVAHGPLTRTRLGELTGLSKPTA
                     AQLLTRLETAGLVRRTGSVTGRPGPSAGLYEIDPGIAHVAALSVGPEGVTALVADIAGT
                     VAGEHRASAEQGEGAAKLVARAVDGALAAAGLGRDALHRTVIGTPGALDPRTGLLRYAP
                     HLPDWRSPALPDELAAITGTPVVVENDVNLAAVAEQHAGAARGFANYVLLWADEGVGAA
                     VVLGGTLLRGATGGAGEIGYMPLPGAPLARGGDDATAGPDGGGGFQMLVASPAVVALAR
                     AHGIDARTAPEALARAAAEPGAGDDVHAELGRRLALGLAAVVAVVDPELVVLSGAVCRA
                     GGERLRAMVEAELTGLALPRPLLRLSTVEDRPVLAGALQTALAAARETVFDTA"
     gene            9565..10065
                     /locus_tag="AS857_RS18485"
     CDS             9565..10065
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146259.1"
                     /locus_tag="AS857_RS18485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FBP domain-containing protein"
                     /protein_id="WP_058044417.1"
                     /transl_table=11
                     /translation="MQSLADNDIRASFINCSKGEARRIALPRNLSELPWEDLDFLGWRD
                     PGAPDRGYVVAEREGKLVGVTLRVPQGGPRSMAKSTMCSLCLTAHPGSGVSLLAARRVG
                     PAGREGNTVGAYMCADLACSLYMRGKKVPQMAMRYKETLSVEERISRMLVNLDDFLGKV
                     ERG"
ORIGIN
        1 gcgcgcagct cgcgggcggc ggtcagggcc gcggtgtagg cgtcgaagta cgcccacagc
       61 tgcccgatga gggcctcggc ggccgcccac cggctcctgg tctcgccggt gagctccgcc
      121 ccctccagca ggcgccgccc cgcatggtcc tggagggcga gcagcgagga ttcgacggcc
      181 tcgtgctccg cgccgagcct ggccagcgca cggtccacct cctcgcgggt caaggccggc
      241 ccggtgggct ccgcgaccgc catcgatccc ctctctcctg tccgcggcgg gcgccgcggg
      301 cgtgtccgtt acggccgtgc tccccggccc gccgtcagtc ccggtacttg ggcgccggcg
      361 gggcggtgat gcccttcagg tcggcggcga gccacttgtc gtacgccttc atccagaggc
      421 ttccggcgcc gcccgcgcgg tagtcctcca gcaccttgtt gacccggcgt accaggtccg
      481 tgtccgcgag gttcatcgcc acgccgtacg gttcggtggt gaacggcttt ccgacgagtt
      541 gcacggacgg gtcctgcgcc gcctggccgg ccgcgagggc cctgtcgttg accacggcgt
      601 cgacctcgcc gagctggagc cgcaccaggc agtcgagctg gttggggacc gtcagcaggg
      661 aggagccgtg cggctcttcc cgcaccttga tctcggccgt ggaaccctcc gccgtacaga
      721 ccttcttccc cttcagcgag tcgtcgaacg cggtcaccgg tgaccccttg gggaccatca
      781 cctgctgccc ggactcgaag taggcggtgg agaacgccac ttgtccgatc cgttcgcacg
      841 tgatggtcat cgtgcgggcg acgatgtcca ccttgcccgt ctgcagggcg gatatgcgcc
      901 ggctcgtggg gatcgcgaga taggtgacct tgccggggtc gccgaggatg tccttggcga
      961 cggcccgtac gaggtcgatg tcgaagccgc tgagctcgcc gtcggcgggg ttgcggtagc
     1021 cccagcggta gctgttctgg tcgacgcccg cgatcagccg gccgcgggcc ttgatgcgct
     1081 gcacggccgc gccgtcggcc ccgcccgcgg gccgcaggct cgcctcgggg tcggtgcact
     1141 gcgcgggcgc cgcggcgtcg ggggcgggcg ccgcgggtgc ggcggcccgg gtgcgggcgc
     1201 gggcttcggc gcgccggggg cgggttccgc cgctccggct cccggcatgc ccgcccctgc
     1261 gggtcccggc gcttccgggc cgggtggacc gggggcttcc ggtaccgggt tcggctcggt
     1321 ggggcggggg ccgggcgggt ggggcagggg gggggggcgg cccccccccc cgtccccccc
     1381 cgccgcaccc gccgtgccct gcgtcggaga cgcccccgtc accgccgacg gcgagctcag
     1441 cggcttcgac atcgacctcg tacgggccgt cgccaaggac atcctcggcg accccggcaa
     1501 ggtcacctat ctcgcgatcc ccacgagccg gcgcatatcc gccctgcaga cgggcaaggt
     1561 ggacatcgtc gcccgcacga tgaccatcac gtgcgaacgg atcggacaag tggcgttctc
     1621 caccgcctac ttcgagtccg ggcagcaggt gatggtcccc aaggggtcac cggtgacccc
     1681 ttggggacca tcacctgctg cccggactcg aagtaggcgg tggagaacgc cacttgtccg
     1741 atccgttcgc acgtgatggt catcgtgcgg gcgacgatgt ccaccttgcc cgtctgcagg
     1801 gcggatatgc gccggctcgt ggggatcgcg agataggtga ccttgccggg gtcgccgagg
     1861 atgtccttgg cgacggcccg tacgaggtcg atgtcgaagc cgctgagctc gccgtcggcg
     1921 gggttgcggt agccccagcg gtagctgttc tggtcgacgc ccgcgatcag ccggccgcgg
     1981 gccttgatgc gctgcacggc cgcgccgtcg gccccgcccg cgggccgcag gctcgcctcg
     2041 gggtcggtgc actgcgcggg cggggctttc ggcacgggcg cgccggaacc ggcatgcgcc
     2101 ccgtccgggc ccctgccatg cgcaccgcct ccccctcgtc ctcccgtacc ggtctcaccg
     2161 gtactccgac agcctgcggc ccacgccgac gacggcaccg gcggagtacc ggtgagaccg
     2221 gtacgggagg acgaggggga ggcggtgcgc atggcagggg cccggacggg gcgcatgccg
     2281 gttccggcgc gcccgccccc ggcgcgccga agcccgcgcc cgcacccggg ccgccgaagg
     2341 cgccgcccgt gggcagcacg gccgcggccg ccgcggccag ggcgcacacg gcggccgcca
     2401 ccgccgccgg gccggcccgc atccgcagcc gtttctccgt ccgggcccct gccatgcgca
     2461 ccgcctcccc ctcgtcctcc cgtaccggtc tcaccggtac tccgacagcc tgcggcccac
     2521 gccgacgacg gcaccggcgg cgccgaggac ggtcagcacg gccgctccgg cggcgaggcc
     2581 ggagaacgcg ccgcgcccgt cgtcggcctc ctgccggaac tcggcctgct cgtgggcgat
     2641 cgcgcgctgc agttcggtgt ccacgcggtc gaaggacgcg ccggtgctgt ccttgtcgcc
     2701 gatcaccttg cgcagggcgt cgtcgtagtc gccgcggtcg tcggcgaggg cgcgtgcctg
     2761 gccgtggcgc tgctgccact cgcggacgtt cttgacggcg gcctcgaccg gatcgcgccc
     2821 ctcggcgtcg tcggcgagcg cccgggcgcg ctccagcagg gtgtccggcc gggccgggcc
     2881 ctggctgccg ccgacgagcc ggcccatgcc gtcgcggtag ccggtctcgt aggcgtcgtc
     2941 gccgttcttc ccgaggacgg cgccccgggc gaccatggcg aggttctcgt cgccgcgggc
     3001 ctggagcgag ccgatgcggg cctcgttgag cgcctgcagg gatttcgcgc cgtgggcgta
     3061 ggaggtctcc aggccggagc gggccaccgc gtggccggcg accagccaca gcaggacgac
     3121 gccggcggcc gcggtggcgg cgagcaggcc gtggttgaag acccggttcg tccggaggta
     3181 gttgcggcgc tgggcccagg cgagggcggc gagcgcgacg gcgccgaggg ccagggcggc
     3241 ccacggccag gacttcgcgt cgtcgtagtc ggagccgagg cggcccgtct cggcctcgta
     3301 caggccgcgg gcggcgggga gcagctcccc gcgcatgcgg tcgcccgcgt agcgcaggta
     3361 ggcgccgccg agcggcaggc cctggcggtt gttggcgcgc gcggcctcca cgaggccggt
     3421 gtagcgcggg agtccctcgt tgagggtggc gatgcgggcg cgggccgtgg ccgagccctc
     3481 ggcgttcgcg gcggccttca ccagcagccg ggaggcgagc gcgatgtctt tctcgtagcg
     3541 ctgccggacc gcgcgcggct cctggccgcc cgcgaggaag ccgctcgccg cggtggtgtc
     3601 ggcgtcggcg agcgagcggt agatgtcggc ggcgtcggcg ctgagcggct ggctggtgcg
     3661 gacgacggag tcggccgcgg cggatctgga ggcgacctgc caggcggtga ccgcaccgaa
     3721 cgcgacgagg accacggcca ggacggcgcc gatgacccgc agcctgccgg gctcggtgac
     3781 cgccgcggcc cgcacgcggt ccagggcctc gcgccaggcc gggccggagg tgctgcggcc
     3841 gggcgctcct cgcggcccgc cggccggctg cggctgtgac acgtgacctc cccctcggtc
     3901 gctgagggca gcccgttgcc cggtgccggc cgggaacagg tgcccggcca gcagtatggc
     3961 cgcaaggact gacaaacaca ccggtgccgg tgcgatcttg ttcggatcgc gatcttcacc
     4021 ggggcccgtg gcggccctcg ttccccatca cgcccgccgg cccggcgcgg ttccccgcac
     4081 cgggccggac gcgtgtgcgc ctgtcgcgtg ccgtgcctta cgcgtaggtg tagtacgagc
     4141 tgtggtcgag catctgggcc ggggtgacgt tgttccacgg acgcatggtg tcgttgaggt
     4201 cgacgacgtt cgtggtgccg gcggccggca ggtacgcgga ctgcgggtgc atgcgctgcc
     4261 agtcggacca cagcttgtcg atgtgggcgt ggtgcagcca gaagaccggg tcgttggggg
     4321 agacgccggt ggccatctgg ccgcccatcc acacgtggac gttgttgtgg acgcggcccg
     4381 cgccgcgcca gccctcgagg tagttgcgga agctgccctc ggaggcgctg ttccacgggg
     4441 ccgtgtcgta gaccggcatg gcgaggacgg cgtccacctg ggcgcgcgtg ggcagcgtgc
     4501 ccgcggcgcc gatctcgcgc tgcaggaagt tgcggttgtc gacgctgacg ttgatggtcc
     4561 acttgcccgt ggagtaggcg aacgggccgg tggtgacctg cgcgtcacgg ctgcggccgt
     4621 tgccgcccat gaagtcggcg ccgaagatcg aggcgttggc cgtgcggtcg acggtccagt
     4681 cccagtaggg cagcgtgacc gaggagtcga tcttctgcag ctcctgctcg aactggatca
     4741 ggaagcggcg gtgccagggg aggaaggagg gggagcggtg accgacgcgg tcgccgttgt
     4801 cggtgtcgct catgatgaac tcgttgtgcg tccggacgaa gccgtcgtac acaccggtgc
     4861 gcttgagctc cagcacggcg ttgacgaagt tgcgcttctc ggtggcggtc aggttcgcct
     4921 ggttcttgcg cactgccatg gggttcgtgt cctcgtgagt gggaagtgtg ggtcggtggg
     4981 gtgcgtgcgg cagtggccgg tcagttcatc gcgatcggga cgagcgtcgc gccgcggagc
     5041 gcgtcgacgg cggcgcgggt gagcgcgagc ggctggtcga agacctggta gtggttcacc
     5101 acgctgatcc aggagccgtc ggcgttctgc atgacgtgca gttccttgcc gtccacggtg
     5161 acggcgaagc cgccgtggtg ggcctcgtgg ccgcccgtca cgggcttgcc gacgatgcga
     5221 cggcccttgt agacctcgtc gaacgcagcg gggccggtgc cgtggccgcc gtgaccggtg
     5281 tggtcggtgg tgtgaccggt gtggtcggcc gcctgcgcgc cggtggcgag cagcgccact
     5341 ccggcggcgg cagcggcggt tcctctgagc acgacgcgtc ggctgacgtt ggacgacatg
     5401 ggtcggttcc ccctcgattg cgcgccgaca aggcgcggaa tggacgatca gtggcggaag
     5461 ttgtcacgaa tgcagccgta aatccctttt gatggcggtt gcttgttgta tttcgtgatc
     5521 aatccgtgcg ggcggagcct cgcatgatcg aggaggggtc ggaaaatggt tggccgattt
     5581 tgggacattg ggtaaatgaa tggtcaacca ctgtcctggg agaaatttct gacgaatgac
     5641 gttgttgacg ccatgtcagg aaactcgtcc gtcgcgaggg cccccggcga aagatcttcc
     5701 ggtccggggt ggggtgtggt gacgcctctc acgatgtgaa catgacgtga agaaggcggc
     5761 ggtatcgcgg attccggcca gggaatgaca gattccgcga taccggtcgc cacgcgggaa
     5821 acaggcgcca aacggacctc gtgccgcccg ccccgcctgc ctcgtgccgc ccccgccgcc
     5881 tgcctcgtgc cgcccgagcc acccgccccg cgcggctcgc cctacgcgtc gccgtagtgc
     5941 gcccgcagcc gggcgtgagc cgtcgccgga gcccctgcgc ggtccagcgc cagcagcgcc
     6001 gcgcccagca ccggcggcgc cgtgaccacc aacggcaccg cctgcggcgc ccgcgacgcc
     6061 agcagcgcca gcacctgctc gtgcagcagc gggtgccggg ccgcgagcac cccgccgccc
     6121 agcaccaccg gcaccgcggc tcccagcagg cccagccgtt ccagtgcgac cgcggccatg
     6181 gcgacgacct cctccgcctg ccgggtcacg agggcgcggg cgaccgcgtc gcccgcggcc
     6241 gccaccgcga acagcaccgg tacgagctcg tgccgccgct ccgccgccac ccggcccagg
     6301 tgcagcgcct cgatcagcgc gggcatgccg tccaggccga agtgcgcggg cagcgcgccg
     6361 gccagcgcgg tcggcccgcc gcggccgtcg gcggcgcgcg ccgccgccca gagcgcctcc
     6421 tccgccaggc aggcgccgcc gccccagtcg ccggagagcc gcccgatcgc ggggaagcgg
     6481 gccgtacggc cgcccggccc gagccccgcg cagttgatgc ccgccccgca caccaccgcg
     6541 acgcccacgg gctcgccccc gtcgggcagg cccgcccgca gcagcgcgaa ggtgtcgttg
     6601 gcgacggcga ccgtccgccc ccagccgcgg gcggcgatct ccgcggtcag cagctcctcc
     6661 tccaccggca ggtcgcagtt ggccaggcac gcgctgacgt gggcggcgta cggcggtgcg
     6721 gccgcccccg cggcagcggc ggcccgctcc acgagcgccg ccaggccgtc caccgcctgc
     6781 cgcacgcccg tccgcgccgg ctcgaacccc ccgccgcgcg aggccccgag cacggcgccg
     6841 tccggccccg ccagcaccac gtccgtcttg ctgttgcccg cgtcgaccgc gagcagtgcg
     6901 ccgctcacgc ccacgcgagg tgctcccggt tgtgcgcgac gagggcgtcg gtcagccggt
     6961 cggcgtggtc gaactgcccg accagcgggt gcgcgagcag cgcccggaag acccggtcgc
     7021 ggcccccctc gagcgcggcc tccagcgcca ggtgctcgta cgccgtgacg ttcgcgacca
     7081 gcccggcgta cagcggctcc agcgggcgta cgggcagggg gtgcgcgccc ctcgcgtcca
     7141 ccgccgccgg cacctcgatc accgcgtcgt cgggcaggaa gggcagcgtg ccgtcgttga
     7201 gggcgttgac gacctggacg tccccggtgt cccgcagcag cgacgcggtc agggcgaccg
     7261 ccgcctccga gtagaaggcg ccgccgcgct tcgccagcag cgcgggtttc tcgtccagcg
     7321 cgggatcggc gtacagggac agcagcttcc gctccatcgc ggccacctcg gcggcccgcg
     7381 agggcttggt gcgcagctcc cggacgacct cgtcgtgccc gtagaagtag cgcaggtagt
     7441 aggaggggac gacgccgagg cggtccagca ccgagcgcgg catgcgcagg tcggccgcta
     7501 cggcgtcgcc gtgctcggcg atcagccgcg gcaggacgtc ctcgccgccc accgtcaccg
     7561 accgctccca ggtgaggtgg ttgaggccca cgtgctccag cgccacctcc tccggggcga
     7621 cccccagcag ccgggcgaac ttccgctgga agccgatggc cacgttgcac aggcccacgg
     7681 cccggtgccc ggccgccagc agcgcccgcg tcacgatgcc gaccgggttg gtgaagtcca
     7741 cgatccaggc gtccgggctg cgccgccgca cccgctcggc gatgtccagc accaccggga
     7801 ccgtgcgcag cgccttggcg agcccgcccg cgccggtggt ctcctggccg acgcagccgc
     7861 actccagcgg ccaggtctcg tcctgctgcc gggccgcctg gccgcccacg cgcagctgga
     7921 gcagcacggc gtccgcgccg tccacgcccg cgtccaggtc cgcggtccag gtgacgcggc
     7981 cggggtggcc ctgccgggcg aggatccgcc gcgccagcgc gccgaccagc tccagccgct
     8041 cctcgtccgg gtcgatcagg acgagctccg tcaccggcag cgtgtcgtgc aaccgcgcga
     8101 agccgtcgac gagttcgggg gtgtaggtgg atccgccacc cactactgcg agcttcaccc
     8161 ttgactcctt tgctggttgc caggtcaaag cggcgaacgg tgcacgccgt ggcggcgaac
     8221 ggctcacgcc gtgtcgaaca cggtctcccg ggccgccgcg agtgcggtct gcagcgcccc
     8281 ggccaggacc ggccggtcct ccacggtgct cagccgcagc agcggccggg gcagggccag
     8341 gccggtgagc tcggcctcca ccatggcgcg cagccgctcg ccgcccgccc ggcacaccgc
     8401 gccggagagc accaccagtt cggggtcgac cacggcgacg accgccgcga gcccgagggc
     8461 cagccgcctg cccagttcgg cgtggacgtc gtcgcccgcg ccgggttccg ccgccgcgcg
     8521 ggcgagggcc tcgggggccg tacgggcgtc gatgccgtgg gcgcgggcga gcgcgacgac
     8581 ggccggggag gcgacgagca tctggaatcc gccgccgccg tcggggcccg cggtggcatc
     8641 gtccccgccg cgcgcgagcg gtgcgcccgg cagcggcatg tagccgatct ctccggcgcc
     8701 gccggtcgcg ccgcgcagca gggtgccgcc caggacgacg gcggcgccga cgccctcgtc
     8761 cgcccacagc aggacgtagt tcgcgaagcc ccgggccgcc ccggcgtgct gctcggcgac
     8821 ggcggcgagg ttgacgtcgt tctcgacgac gacgggcgtg ccggtgatcg cggcgagctc
     8881 gtccggcagg gcgggggagc gccagtccgg caggtgcggg gcgtagcgca ggaggcctgt
     8941 gcgcgggtcg agggcgccgg gggtgccgat gacggtgcgg tgcagcgcgt cacggccgag
     9001 cccggcggcg gcgagggcgc cgtcgacggc ccgggcgacg agcttcgccg ccccctcgcc
     9061 ctgctccgcg gacgcgcggt gctcgcctgc caccgtaccg gcgatgtcgg cgacgagcgc
     9121 ggtgacgccc tcggggccga cggacagcgc ggcgacgtgg gcgatgcccg ggtcgatctc
     9181 gtacagcccg gcgctgggcc cgggtcgtcc ggtgacgctg ccggtgcggc gcaccaggcc
     9241 ggcggtctcc aggcgggtga gcagctgggc ggcggtgggc ttggacaggc cggtcagctc
     9301 gccgaggcgg gtgcgggtga gggggccgtg cgcgacgagc agctccaggg cggcgcggtc
     9361 gttcatggcg cgcagcacgc gcggcgtccc cggggttccg gccatctcgt cgtccctgac
     9421 tgttggggaa acctgactgt taggaaactt tcctatcgat gcccgggagg ctagggaatc
     9481 acagggcagg gcgtcaatcg cccgggacgg cccggcccga aaatcatgcg acgatccgcc
     9541 cgaacggtgt taatttccct gaccatgcaa tccctcgccg acaacgacat acgcgcgtcc
     9601 ttcatcaact gctccaaggg ggaggcgcgc cggatcgccc tcccgcgcaa cctctcggaa
     9661 ctcccctggg aggacctcga cttcctgggc tggcgggacc cgggtgcgcc ggaccgcggc
     9721 tacgtcgtcg ccgagcgcga ggggaagctc gtcggcgtga ccctgcgggt gccgcagggc
     9781 gggccgcgga gcatggcgaa gtccacgatg tgctcgctgt gcctgaccgc gcacccgggc
     9841 tcgggcgtct ccctgctcgc cgcccggcgg gtcggcccgg ccgggcgcga gggcaacacg
     9901 gtcggcgcgt acatgtgtgc ggacctggcc tgctcgctct acatgcgcgg caagaaggtc
     9961 ccgcagatgg cgatgcgcta caaggagacg ctgagcgtgg aggagcggat ctcccgcatg
    10021 ctcgtgaacc tggacgactt cctgggcaag gtcgagcgcg gctaggcgga gcggcgccgg
    10081 gccgcgggcg gggctatgcc gcgccggccc ggaccgcccg gcggcggggc cgccgggccg
    10141 ccgtcacttg ccgagggccg cgcccggcac cgccggcggt atgggcgtcg tggcctggga
    10201 ctgcggggac gccgggttcg ccagggcgga cggagcggcc acggccgcca tcgggtcgga
    10261 cccgtccgtc tccagattcg cctccggcgg gcggcccacg atgcggatgc cctccgcgtc
    10321 cagcgcctgc ttgatgcgcc agcgcagctc gcgctcgacg ccgagcgcct tgcccggcat
    10381 ggtcttggcg gagacccgg
//
