LOCUS       NZ_LNBE01000003        20699 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_3, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000003
VERSION     NZ_LNBE01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2630732)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 2630732)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2630732)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2580621
            Orig. end                         :: 2601320
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20699
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="26"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20699
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20699
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10699](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10699
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     misc_feature    100..114
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 23.96"
                     /tool="antismash"
     gene            292..696
                     /locus_tag="AS857_RS16925"
     CDS             292..696
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944288.1"
                     /locus_tag="AS857_RS16925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="WP_058043904.1"
                     /transl_table=11
                     /translation="MTTTQNDAIYSILDNNLSRIAGIQGAVLLSNDGIKLSAYMLERDQ
                     AERVAAASSGIASTMKAISREIDGGGVIRQLVEMEDRYLCIVGCGSGSTLIVVASRKAR
                     LGELGGETVRAAQALGEWLDTPERSQAPTS"
     gene            696..1064
                     /locus_tag="AS857_RS16930"
     CDS             696..1064
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386277.1"
                     /locus_tag="AS857_RS16930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF742 domain-containing protein"
                     /protein_id="WP_058043905.1"
                     /transl_table=11
                     /translation="MAADPQPGRRRRTRLYALTDGRTAASRHGLTMDTVITAAPGAGHG
                     HGSLPTEWQEILAMCPSPNGRAVAEIAARMRMRLTPMVVLLGELLERGLIRHRPPLDAS
                     ETSNVHLLMKIRDNLARL"
     gene            1051..1644
                     /locus_tag="AS857_RS16935"
     CDS             1051..1644
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923498.1"
                     /locus_tag="AS857_RS16935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP/GTP-binding protein"
                     /protein_id="WP_058043906.1"
                     /transl_table=11
                     /translation="MPDYDSAAAPEASPVKILVAGGFGVGKTTLVEAVSEIDPLRTEER
                     LTSAGVGVDDLDGIESKTATTVAMDFGRITLTDAGVALYLFGTPGQERFWFMWDDLLYG
                     ALGAVVLVDTRRLDRSFPAIDFFENRGLPFIVGANCFNGEKPYTPEEIKAALHLRDPDT
                     PVLLLDARSREDVRSCLLSLLDRLIAQARLTAPA"
     gene            complement(2492..3757)
                     /locus_tag="AS857_RS16940"
     CDS             complement(2492..3757)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016325053.1"
                     /locus_tag="AS857_RS16940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_079110394.1"
                     /transl_table=11
                     /translation="MRRTRPRLGRGLGALVATATAAVGLAATPAAATPAASGQTVQPGQ
                     ACTAADLGTRHPIIKSAEVAPTITHFKGWYVTEGSTGNQSVNTNTTETISVSVGLNGTV
                     QGSFAVEALGSVGASLGLNVQVNYTSTSSTSTSVTWNFRNPGYYGLYKGTKKVTGTYGS
                     LNCNRTQQGDGSWALKWTEGPDTGSYTTFTTLEEGAVRCEDKVPANSIMRKAQDLLDCG
                     SATATAKHPAGPVPSAKAEAAKAGHEAEEAAKAEKSVRATGSPAPATRAALNCQSAVNK
                     IDVPGKPLNWSAPLLANDGIRLRQSNWTSAHLDNWRLCNVTERNGVIEASLWNWGNGGC
                     ATIPADIANREQAYLTTATCGEDDLQRFYIYRDVPGSSKIGLQNKYTGSMMGHDRYADG
                     ELIRQYSSGRQDGTGTYTLTRV"
     misc_feature    complement(3951..3966)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.93"
                     /tool="antismash"
     gene            3973..5238
                     /locus_tag="AS857_RS16945"
     CDS             3973..5238
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127671.1"
                     /locus_tag="AS857_RS16945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_245699976.1"
                     /transl_table=11
                     /translation="MAAIASLALTAALLAVGASPAAAAPTAPQGYGNCTAVPAGSDLQK
                     RGAAWSCSGSDSTDRRVPAPVLAAPDLARAPADGANAVKLCLKDSSERVVSDRHAYCTR
                     MFIREVLLNKENVAIGEGHISVLAVGLLDSYAGKWTESIIVVPGDMTGEVTGVEVSLDS
                     TCSGMCSTQGPAWGGGYATVEKGGDNQTGTITYLSSVAAKTDISYINLTYTVRAAAVGG
                     VGVPGYDTAIYDGPQVRCDATIGDTRPVPGCIVFAGHTPNVTFSRAKYRGAAVAYEWAQ
                     KNLTGQYGTPGHLLSRYIDPLDPEAEDRRALTCDRGPYKFPRGATGILNDSCDEYPFAR
                     SRQGGNPGDQCVDITPRPVGGVWDVSGVTVDRGTGVSPYNAPCIRAHVDNKDNTAAGGE
                     LGRAVQSDRILDSEWYEVIIVP"
     gene            complement(5277..>5763)
                     /locus_tag="AS857_RS16950"
                     /pseudo=""
     CDS             complement(5277..>5763)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981454.1"
                     /locus_tag="AS857_RS16950"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="EGLGRSRGGLTTKIHLVGEGGPAAPGPTDHTRTMGRRASDDSGPG
                     ADPRPAAEGRAPAHPPGPPQRRSCLQLSAQPPLPAAPSGQAHHP"
     misc_feature    complement(5716..5718)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5771..6118
                     /locus_tag="AS857_RS16955"
     CDS             5771..6118
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS16955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058043909.1"
                     /transl_table=11
                     /translation="MPFLQAIASKVAEDAYGQARQLVRRLLRRNDGNEEEEKEKSSPPS
                     SSDVETGPGLAILQDPDAGLTLFLWSNASDEALRALSSLDLNELTLRRPDQGQVHLVWH
                     AESGTWHVRGS"
     gene            complement(7543..8928)
                     /locus_tag="AS857_RS16960"
     CDS             complement(7543..8928)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS16960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_058043910.1"
                     /transl_table=11
                     /translation="MARHPLKVARESLGLSQLGYARLIARAHDELGFGPRMVRTRHTVS
                     HWEAGRNEPELTAQLAIARVHRVPEEEVARLGWPHWLHLATDDTALLNQPWTPRGAIGA
                     LHSTARLADARPRSYLTVTGPALDFQIKKSLAALASPQPPPTRDGRPVTPDVLAGIEAR
                     IEALELQEIATPVTPMALYVAARAEHRLVTGLLTGHGYDAKTGAWLLLLATRTAALCEW
                     LSGCLGEEARAERYALAAIRAATAAGSRRHVASCMIDLAFRHLVAGDPKDMLSLVHAAR
                     AIVRRPPAGLAVTLHTREAQALARLGDLTASTRALDRATSTLAAKAADADPAADLLCVN
                     VGEEWLAVSSGAAWLHLGRPEKALPHFTTLLDDGPASRAPGLPSPYTARRLLYVVDAQL
                     ALGELDAAAHSAHRAVALVGCLPPGLARQFQQRFAHHSTEPAVRDLIEEIRSPDERHPS
                     PLR"
     gene            9175..9987
                     /locus_tag="AS857_RS16965"
     CDS             9175..9987
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /locus_tag="AS857_RS16965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_058043911.1"
                     /transl_table=11
                     /translation="MESQFDSTVAAYAQSMSALPFREHVEAHSLLTAIGPVAGLSVLDL
                     GCGTGSYTRYFHTAGAGRVVGLDASLSMVESARGEEERERRGITYVHRDATRPLKDTDV
                     DGLTGAFDLVTSVYVLPYAMSEEELAAMCATARRSLSPAGGRFVAMTLNPDFATDPEWY
                     RHYGMRLTTDRPGGEGATVRLTAWISGQTIDVEARRWSAGAHERALCAAGFDSVSWVRP
                     EVSEAGRRRFGDDFWSKYLACPHALIIDATAEPAPDTDSGSPSGRETD"
     gene            10001..10699
                     /locus_tag="AS857_RS16970"
     CDS             10001..10699
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR04539.1"
                     /locus_tag="AS857_RS16970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_058043912.1"
                     /sec_met_domain="CDPS (E-value: 1.5e-61, bitscore: 205.0,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFLVQPCTAESRRILARAEHLLIGLSPWNGYYKPRRVEALVDWA
                     CANFASVDVFVPGYEAAHTLTAAGFEPREAVHRTRRAISQLRNPAQRALRRADIPNPGA
                     RFHSWTQLANRPPYTRMLERVHQAYRTDEAVRRACRLTARGAVLHAAEREPSERQIDQA
                     VGYALAELPLVLDGPAIFGTESSVFVYHREMDLLDPFITGRATRLRPASGQGYAVVRPA
                     DDRCVPSHRL"
     misc_feature    10694..10696
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            10723..11931
                     /locus_tag="AS857_RS16975"
     CDS             10723..11931
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /locus_tag="AS857_RS16975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_058043913.1"
                     /sec_met_domain="p450 (E-value: 3.6e-21, bitscore: 72.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQPTTDPDLPLRYPFPPGELGAPPLLLGWARKKQPVCPVVLPSG
                     DRVWMVTRKDDITRVLTDPGFSRDLTYPGAPRFVGDDFTSVPGGIFNLDPPDHTRVRQV
                     INRFYTRSAVERHRPVIEGHAVRLLDDMAHGPNPADLMPAYAAALPLHVAREILAVPPE
                     LHSDYHSCFRTQTHLAASAAAVAAATASITDFTREVIETKRRDPDPDEPIGALIRARSG
                     GLISEGELIGTTSYLFVTGSEPLTAPLGTGVITLLTHPRQLRECIKAPDLWPKAVEEIL
                     RYHHNGVLGMPRIATEDVTLHDVTIRKGEGVCCPMLGATHDPAHYPNPSRFNIHRTTDA
                     SATFGAGPHFCLGAPLTRLFLHTAYRALFTRFPSLFLAVPEREIPWEDNLLFIKPASLP
                     IAW"
     gene            complement(11979..12717)
                     /locus_tag="AS857_RS37770"
                     /pseudo=""
     CDS             complement(11979..12717)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558180.1"
                     /locus_tag="AS857_RS37770"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGRGDLSNEEWARLGPHLPRNVGRHMGSDSAGRPGLCRRARPH"
     gene            12832..13137
                     /locus_tag="AS857_RS16980"
     CDS             12832..13137
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009069107.1"
                     /locus_tag="AS857_RS16980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058043914.1"
                     /transl_table=11
                     /translation="MEHLPVDVRASLRLFAFYLANGTIDLELLDGFDYRSTVIHSGSSL
                     EQVFAIYGNVLQIDANGMVLNDGDAQYRVAQWIRACCDPSYRVEPPFEAWETELHL"
     gene            complement(13163..13994)
                     /locus_tag="AS857_RS37775"
                     /pseudo=""
     CDS             complement(13163..13994)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044031.1"
                     /locus_tag="AS857_RS37775"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MICRHELSDVEWELVQPLPPRPVLGGRVWMTGWCSTGSCGGFAPG
                     SPGGMCPSDTGRGPVSTPVSAGGRRTAPSSGCSRPRKRRPTRPVTSTGWCQSTPASSAP
                     TSTLPEPEKGGPEPCPRTVPRWTDQQNSPGLRRLWPSAHVRADGRERQRLHPVHRRYGR
                     DPGPPNRAGTTPDAAGPRHLRQGLQLRGDPHLAAKARHRPRHSRAGRSGRKPPPTGKRR
                     RPPVGVRSGLAQAAQHGGAVLQPAEAVTRPSHPL"
     gene            complement(14259..15722)
                     /locus_tag="AS857_RS16995"
     CDS             complement(14259..15722)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1206: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /locus_tag="AS857_RS16995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ISL3 family transposase"
                     /protein_id="WP_245699995.1"
                     /transl_table=11
                     /translation="MIDAAGYGPPGPCPQCQHPAARVHSRYWRHIAGLPVGGHRMIIRL
                     RVRRFFCDQSQCRRRTYVEQVAGLTKPRLRTSTVARSAMRAVAVELGGRPGQRLCAKLR
                     VHGRRTTLLRQLAAQPVPARAPRILGIDEFAFRKGRTYGTVLVDVESSRPLDVLPDRET
                     STVATWLQEHPGAEIVCRDRLMAFTKAIRQAGPDALEVADRWHLLQNLSAAVEKTCRRH
                     HECLRRPAGLGSNPPSATPETPLLDRIRRRHGEVNELAATGMSLSAIGRRLQLDRKTVR
                     RYRNKDLAGLLASAQDRGHGVLDPFIEHVQHRFQAGCTSSMQLYRELLALGYTGGYHVV
                     NRYVMAIREGIAVPARSVTFRPRDITSWIMRPEETLSPADVAQLETVRNACPEIALACD
                     LAREFTNMLRHRRGYLLRDWIQKAELGGPDAIGIFADSIRQDLHAVTAGLTLSYSSGIV
                     EGHVNRIKTIKRQMYGRASFALLRARVLL"
     gene            15606..16607
                     /locus_tag="AS857_RS17000"
     CDS             15606..16607
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039374.3"
                     /locus_tag="AS857_RS17000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6193 family natural product biosynthesis
                     protein"
                     /protein_id="WP_058043916.1"
                     /transl_table=11
                     /translation="MAADGQPSDVTPVPAVDACGGMLTLRTWSWGSVASRVDHQGVVRD
                     HGLAHHEVHAREEDVFECSQLHEDVIPWRNGPAVQSFERQPSGQWQTSEVNSDLYPELA
                     AAGSLAAALEQLAADLGVDLVVVPGGWGALVSAGIAASVPERKPLSVHIGAESRWFGVS
                     GWSQGVELITGSTPDLADVVRAGVAWGQGRSLRELRAELPFLHSSERAEAHDRGPAAVV
                     ELQWRLMREQAAEAPDFPEFGALVEAAHVERRLRELYVFSSHWTLGFSSCTGFPFENEV
                     AIAPSYGGGPYRVLKHPHADVISEAATAEEAVALAVTRLPAGLGPAVTGTAK"
     gene            complement(16820..17635)
                     /locus_tag="AS857_RS17005"
     CDS             complement(16820..17635)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019436326.1"
                     /locus_tag="AS857_RS17005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058043917.1"
                     /transl_table=11
                     /translation="MYKTSAALATARWPLAASTETDWLRELAAEDGLTGFMPPALPDAA
                     WVLHAMYEHELGPFDMSYVEYLQAFLNRNSTSPEIIPGLDPADVFTDPSDPFAKSTGEH
                     PGPRYRRLRWAELARRTGDPVAAEGRLPCHTSFPSLRESGGWPVGITGPSEGSLDRSDW
                     NRLVEILTEHSPQGADTRCLAYYTPLVLGAEDFDNLHVRAGRLADAKALYDHPEEDSWS
                     PSNLWAHDRSWVLCTDYDLWATKVAGPAPLVEALLNDSEIEAVRLPWAP"
     misc_feature    17879..17892
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.2"
                     /tool="antismash"
     misc_feature    complement(17880..17893)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.41"
                     /tool="antismash"
     misc_feature    18423..18438
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.34"
                     /tool="antismash"
     gene            19046..>19378
                     /locus_tag="AS857_RS41495"
                     /pseudo=""
     CDS             19046..>19378
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /locus_tag="AS857_RS41495"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSLLPQVRCTYAPRGRTPILRHRLNWKRASMAAALGYHASDPGRG
                     PRLCFHLKPGSYDTTSLIEALEQVKTFYAGEPVVLVWDGLSAHWSRDMRAWAAEQDWLT
                     LERLPAY"
     gene            complement(19589..>20376)
                     /locus_tag="AS857_RS41500"
                     /pseudo=""
     CDS             complement(19589..>20376)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668654.1"
                     /locus_tag="AS857_RS41500"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="helicase"
                     /pseudo=""
                     /transl_table=11
                     /translation="TQLIERGILAGFEIDVLEIRGPSPRLRRVGRGPAGLAPGALADGA
                     PGARGRAQPAHRDDLPPEGGGGRRGPAPRASIDAEFYELETGRHVPPDRVWSAWLCGDH
                     LVTERREVLRQFAGGIDATNRRVHRAFLASVRVLEEGVDITGERSVEAICFADTRGSQV
                     ESCRTSAAHCDSTATAARRSPASSCRSSSSPARTRPTWSPPPPTAPS"
     gene            20354..20509
                     /locus_tag="AS857_RS40035"
     CDS             20354..20509
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS40035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_160330267.1"
                     /transl_table=11
                     /translation="MPRSISCVVTCGTLPTIETTATGNPLLVVRRFLNTVLVRAPGPGL
                     RLRAAA"
ORIGIN
        1 cgcgccgggc ctgccccgcc gcctgcggaa ggagcaggca cgggacgccg ggaacacggc
       61 gccggaggta ccgccacggc ccggttccgg ggacacctcc ggccggccgg ccctccccga
      121 gcgcatcccg caggcccact tggcccagca gctccgcgag ccgcgcgcgg ccggaacggt
      181 ggacgtgggc caggacgccg ccacccccga agaggtggcc gacgcctggg ccgactacga
      241 agacagcacc aggcaagtgg aactcgagct ccgacaggac cagccatgac catgacgacg
      301 acacagaacg atgcgatcta cagcatcctg gacaacaatc tgagcaggat cgcggggatc
      361 cagggagccg tactcctctc caacgacggg atcaagctca gcgcctacat gctggagcgg
      421 gaccaagccg aacgggtcgc cgccgcgtcc tccggtatcg cgtccaccat gaaggccatc
      481 tcgcgggaga tcgacggcgg cggagtgatc cgccaactcg tcgagatgga agaccggtac
      541 ctgtgcatcg tcgggtgcgg atcgggcagc accctgatcg tggtggcctc ccgcaaggca
      601 cggctcgggg aactgggcgg cgagacggtc cgcgccgcgc aggcgctcgg cgagtggctg
      661 gacaccccgg agcgttcaca ggcgcccacc tcgtaatggc ggccgacccc cagcccggac
      721 ggcgccgccg gacgcggctg tacgccctca ccgacgggcg tacggccgcg tcccgccacg
      781 gcctgacgat ggacaccgtg atcacagcgg cccccggtgc cggccacggt cacggcagcc
      841 tgcccacgga atggcaggag atcctcgcca tgtgcccgtc gccgaacgga cgggcggtcg
      901 ccgagatcgc cgcccggatg cggatgcgcc tgactcccat ggtggtcctg ctcggcgagc
      961 tgctggaacg cggcctgatc cgtcaccggc cgccgctgga cgcctccgag acctccaatg
     1021 tccacctgct catgaaaatc agggacaacc ttgcccgact atgacagcgc cgccgcgccg
     1081 gaagcgtccc ccgtcaagat cctcgtcgcc gggggcttcg gagtcggcaa gacgactctg
     1141 gtcgaggccg tctccgagat cgatccgctg cggaccgagg agcggctgac gtccgccggc
     1201 gtcggcgtcg acgacctcga cggcatcgag agcaagacgg cgaccacggt cgcgatggac
     1261 ttcggtcgca tcaccctcac cgacgccgga gtggccctgt acctcttcgg cacaccgggc
     1321 caggaacgct tctggttcat gtgggacgac ctgctgtacg gggcgctcgg ggccgtcgtc
     1381 ctggtcgaca cccggaggct ggaccgcagc ttccccgcca tcgacttctt cgagaaccgc
     1441 ggactgccgt tcatcgtggg cgcgaactgc ttcaacggcg agaagccgta cacccccgag
     1501 gagatcaagg cagccctgca cctgagggac ccggacaccc ccgtcctcct gctcgacgcc
     1561 cgctcccggg aggacgtccg cagctgcttg ctctcgctgc tggaccggct catcgcacag
     1621 gcacgcctca ccgcgcccgc ctgacggcgg acggccggag gcgtcacggt ggcgacagcg
     1681 gctccagcac gacccaccgg aaggccggcg ctctgggaac agagggactc caaaccgccg
     1741 gtgcgcgggc ccaagttctc caggatcccc agccgattcg cggcgcggga tgccggcccg
     1801 cggcgctacc gtcaggtgga ttcgcaggcg gcggccgacc caccgcagaa tgcgcgacgc
     1861 ccttgatgac ctggggcgtc tggccaatcg catgtcggtg ccggaggtga tcggcaggtt
     1921 tctgcctcgc cgagaacagc ggcggccgca ggcgcggagg tgacgcccca ccagcgacag
     1981 gaaatcgaca agtgggtaga gcgcgcgcgg ggcatgaaga tccagcccgg cgccgcccac
     2041 gcccgtagaa tccgtggtgg ctggccggga ggactttacg aagccggcgc ggcccgggac
     2101 ctcgccgggt aagcaccttt cctcccagca tgaccggcgg cgcgctgtgc cgcctcaagc
     2161 agggtccaag ggtaaatccg agaaacgcgc ggcatctgcg cggctcgaag cccagccgac
     2221 taggccctgt ctgatgggtc ggtgacgaac cgagacgaga actcgtgggc caccgtcaac
     2281 agaacctcgt atacgcatgc gttgaccgca tttctgtagt cgggttgaag cgcgaaccat
     2341 cctggaaggg gttcgccgca tgcaacaggg gacgttcggc tacgggcacc ggtccccaag
     2401 gctgggttcg gggtagagac gcggaaggcc ccgcacggaa ccgggaggcc gggggttcac
     2461 gtgcggggcc gcgcttacgg ggtgggggcc gtcagactct ggtcagggtg tacgtacccg
     2521 taccgtcctg cctgccgctg gagtactggc ggatcaactc cccgtccgcg taccggtcgt
     2581 ggcccatcat ggagccggtg tacttgttct gcagaccgat cttcgagctg ccgggtacgt
     2641 cgcggtagat gtagaaccgc tggaggtcgt cctccccgca ggtggccgtc gtcaggtagg
     2701 cctgctcccg gttggcgatg tccgccggga tggtcgcgca tccgccgttg ccccagttcc
     2761 acagggacgc ctcgatgacg ccgttgcgct cggtcacgtt gcacaggcgc cagttgtcca
     2821 ggtgggcact ggtccaattg gattggcgca ggcggatgcc gtcgttggcc aggagcggtg
     2881 cgctccagtt caggggcttg ccggggacgt cgatcttgtt gacggcggat tggcagttca
     2941 gggcggcccg cgtggcgggg gccgggctcc cggtggccct cacgctcttc tcagccttgg
     3001 cggcttcctc ggcttcgtgc ccggccttgg cggcctcagc cttcgccgag gggaccggcc
     3061 cggccgggtg cttcgcggtg gcggtggcgg agccgcagtc gaggaggtcc tgcgccttgc
     3121 gcatgatgct gttggcgggg accttgtcct cgcagcgcac cgcgccttct tcgagggtgg
     3181 tgaaggtggt gtagctgccg gtgtcggggc cctcggtcca cttcaacgcc cagctgccgt
     3241 cgccctgttg ggtgcggttg cagttgaggc tgccgtaggt gccggtgacc ttcttggtgc
     3301 ccttgtagag gccgtaatag ccgggattgc ggaagttcca cgtgacggac gtcgacgtgc
     3361 tgctggtgga ggtgtagttc acctgcacat tcaggcccag gctcgcgccg acggagccga
     3421 gggcctcgac ggcgaagctg ccttgaacgg tgccgttgag accgacggag acggagatgg
     3481 tctccgtggt gttcgtgttg acgctctggt tgccggtcga gccttcggtg acgtaccagc
     3541 ccttgaagtg cgtgatggtg ggggcgactt cggcactctt gatgatcggg tggcgggtgc
     3601 cgagatcggc tgcggtacag gcctggccgg gctggactgt ctgcccggag gcggccgggg
     3661 tggcggcggc cggggtggcg gccaggccca cggcggccgt cgcggtggct acgagcgctc
     3721 cgagccctct gcccaggcgg ggtcttgtgc ggcgcatcgt tgtttccccc tgcgtgtggt
     3781 ctgtgaggtg agtgtgagtg aaaatagcgc cgccgggagc ggcaggataa taccacaact
     3841 ggccaaatga aatcaagaac tgtcccgttc tcggttggca tgaacgagag ttgcccggtt
     3901 ccataccttc ggagtgcccg ccaaggggcc accgacggaa gggaccacct tgtgaaacac
     3961 ctcagacgcg gcttggcagc gatagcctcg ctcgccctca ccgcagccct gctcgccgtg
     4021 ggcgcctccc cggccgcagc cgccccgacc gctccccagg ggtacggaaa ttgcacggcc
     4081 gtccccgccg gatcggatct gcaaaagcgt ggtgcggcct ggtcctgctc cgggtcggac
     4141 tctaccgacc gccgtgtccc cgcccccgtc ctggccgccc cggaccttgc ccgcgccccc
     4201 gccgacgggg cgaatgcggt caaactatgc ctcaaggact ccagtgaacg cgtcgtctcc
     4261 gaccggcacg cgtactgcac gcggatgttc atcagagaag ttctcctcaa caaggagaac
     4321 gtggcgatcg gcgagggcca catcagcgtt ctcgccgtgg gcctgctcga ctcctacgcg
     4381 gggaagtgga ccgagtccat catcgttgtc cccggcgata tgacaggcga ggtgaccggc
     4441 gtggaggtgt ccctggattc cacgtgcagc ggcatgtgca gcacgcaggg accggcctgg
     4501 ggcggcggat acgcgactgt ggaaaagggc ggcgacaacc agacgggcac catcacctac
     4561 ctgtccagcg tcgccgccaa gacggacatc tcctacatca acctcaccta caccgtgagg
     4621 gccgccgccg tcggcggggt cggcgtcccc gggtacgaca ccgcgatcta cgacggcccc
     4681 caggtgcgct gtgacgcgac gatcggcgac accagacccg tccccggctg tatcgtcttc
     4741 gccgggcaca cgcccaacgt gacgttctcc agggcgaaat accggggggc ggccgtcgcc
     4801 tacgagtggg cgcagaagaa tctcacgggc cagtacggca cccccggcca cctgctgtcg
     4861 cggtatatcg acccgcttga cccggaagcc gaggacaggc gtgccctgac ctgtgaccgt
     4921 gggccgtaca aattcccccg gggcgccacc ggtatcctca acgactcctg tgacgagtac
     4981 cccttcgccc ggtcccggca gggcgggaat cccggcgacc agtgtgtgga catcaccccg
     5041 cgccccgtcg gcggcgtatg ggacgtctcg ggcgtcaccg tcgaccgggg caccggcgta
     5101 agcccgtaca acgcgccctg catccgggct catgtcgaca acaaggacaa caccgctgcg
     5161 ggcggcgagc tggggcgggc ggtgcagtcc gaccgcatcc tcgacagtga gtggtacgag
     5221 gtgatcatcg tcccgtagga ggcagaccag gggccggtta ggttccgtct gacgggtcat
     5281 gcacgaagcc acaggcgtat ggccgcaacg gtcacagtgc cgtagaagac ataggcccgc
     5341 ttgtcgaacc tcgtgacaac cgcccggttg atcttcaact ttccgattag ccgctccact
     5401 tcgttcctgc gaacgtagcg ggccttctcg aaactggtgg gccggctgcc tcggctccca
     5461 cggcgcaagc gattggcctg ctgattcctc ggctcaggga tggtgtgctt gacctgacgg
     5521 cgccgcaggt agcggcggtt gcgccgagag ctgtaggcac gatcgccgct gaggtggtcc
     5581 gggcgggtgc gcgggtgccc gcccttcggc cgcgggacgc ggatccgctc caggaccgga
     5641 atcatctgag gcgcgtcgcc ccattgtccg ggtgtgatca gtagggccag gggccgcagg
     5701 gccgccctca ccaactaagt ggatctttgt ggtgaggcca ccgcgggagc ggccaagacc
     5761 ttccgccgtc atgccctttc tccaagccat cgcctccaag gtcgccgagg acgcctatgg
     5821 gcaggcccgg cagctggtcc gccggctgct gcgcaggaac gacgggaacg aggaagaaga
     5881 gaaggagaag tcgtcgcccc cctcgtcgtc cgacgtggaa accggaccag gcctggcaat
     5941 tctccaggat ccggatgcag gcctcacact cttcctgtgg tcgaacgcgt cggacgaagc
     6001 actccgtgct ctttcatccc tggatctgaa cgagctgacg ctccgtaggc cagatcaagg
     6061 gcaggtccac ctggtgtggc acgcggaatc gggtacatgg cacgtccgcg gctcttgact
     6121 cgggcacgac tccagcagtg tgctcggcag tcgtcttcgc tgcggcccta gcgccgcttt
     6181 tcgcaggccg agcgcgtggc ggggcccgcc gtttagcgcg gtgccctggc agttcaccgg
     6241 ctaaggcgtc cgcgaggtgc ttgtccaccg tggccgtgcc gttggcccaa ggatcctcta
     6301 ctgctgacta tcgtgaaggt ccccggcagc gctcacctgc acacgaaagg tccaggcggt
     6361 tgggaaggat caaggccact gcgattgtgg tgggtggagt gctgggcgcg ggagctgcgg
     6421 tggccggcat tttcacgtgg gctggcatcg aggcgccttg gacgagcaag ccggggccga
     6481 cagggacagc aacagagcca tcggcagaag tcggccgccc tctgaacgtc tacgcatatt
     6541 gccggtacaa ggggtacgcc tcctcgcgcc cggaggaaga gctccggact gtccgggact
     6601 ggacgtgcgt acgtgcggac ggcagcttgg aaccgatcac gctcaacggc cacctctcct
     6661 ggaacgaggc ctgccagcac cagtacggcc ctgcggtcgt tgctacgaac actgaccccg
     6721 gaaggcaatg gggcagtgtg aggtgcgtga gaggctgagc ggaacgccgc ctgcgcgagt
     6781 gctgtagatc aagatggccg caccgacccc gtacggcggg acgttggagg gtcaactctc
     6841 gaagaaggtg gtggcgtcgc cgggcggcgt cgtgaagtag ggcaccgtct cgatctctga
     6901 tccgcactcc tcgcaggctt gaatctcgac ggtctggtcc tggctgagcc gtccgggcgg
     6961 gaagtcggtg tatccgcacc tagagggtct gtccgatggg tcgcacgcga ggcgcgggcc
     7021 atcggatagg ccctgggttt cggcctgatt ggctggaact gcgactggct agggctgacc
     7081 agggagtcct gcgcgagcag acccccccgg ccgccctgtt gagtagcggt cagatgcgag
     7141 tcgtcaacgt cctgcgccgc tgaagacctg tggtcacttc ggggcgggtg ccagacgaat
     7201 catgtgggga cgggcccgga tccgcatcgg cggaggtggt ggtgaagtcg ccgggggccg
     7261 ggggccgtcg tttgacgggc cgggtcagcc gccgggtcat caggtgaccg cccagttggt
     7321 gtgggcttcg ccgatggagt gggcggacgt cattctggcc atggatcacg acaacctcgc
     7381 caccctgctc cccctcgcgg acgaccggat ctccctcaag ctggcgctgt acctcggcga
     7441 ccaggacgtg cccgacccct ggggtcagct ggagaacgcc ttaccatctg cgctgaactc
     7501 agcgaggcgg gcgcccggcc gccaccttcg cagggtgatc ccctatcgga gaggacttgg
     7561 gtggcgctcg tcgggggagc ggatttcctc gatgaggtct cgtacggccg gttcggtgga
     7621 gtggtgggcg aatcgctgct ggaactgccg ggcgaggccg ggtggcaagc agccgacgag
     7681 ggcgacggcg cgatgggcac tgtgggcggc ggcatcgagt tcgccgaggg ccagctgcgc
     7741 gtcgaccaca tacaggaggc gtctggcggt gtacggggag ggaaggccgg gcgcgcgcga
     7801 agcggggccg tcgtcgagca gtgtggtgaa gtgcggcagt gccttctcgg gccggcccag
     7861 gtggagccag gcggcgcccg aggacacagc cagccattcc tcgccgacgt tgacgcacag
     7921 caggtcggcc gcggggtccg cgtcggccgc cttggcggcc agcgtgctgg tggctcgatc
     7981 cagcgcccgg gtactggcgg tgaggtcacc gagacgggcg agggcttgcg cctctctggt
     8041 gtgcagggtg accgcgaggc cggccggggg gcgccggacg atcgcccgtg cggcatggac
     8101 cagggacagc atgtccttgg gatcgccggc tacgagatgg cggaacgcca ggtcgatcat
     8161 gcaggaggcg acgtgacgcc gcgagcccgc ggcggtggcg gcgcggatcg cggccagggc
     8221 gtaccgttcg gccctggctt cctctcctag gcagccactg agccattcgc acagcgcggc
     8281 ggtgcgtgtg gcaaggagga ggagccaggc tccggtcttc gcgtcgtagc cgtggccggt
     8341 caggagcccg gtgaccaggc ggtgttccgc gcgggcggcg acgtacaggg ccatcggggt
     8401 gaccggtgtc gctatctcct gaagttcgag tgcttcgata cgggcctcga tcccggccag
     8461 gacgtcgggg gtgacggggc gtccgtcccg ggtgggtggt ggctggggcg aggccagggc
     8521 ggccagtgac ttcttgatct ggaagtcgag ggcggggccg gtgacggtga ggtaggagcg
     8581 gggccgtgcg tccgcgagcc gggctgtgct gtgcagggcg ccgatcgctc cccggggtgt
     8641 ccacggctgg ttcagcagtg cggtgtcgtc ggtggccagg tgcagccagt gcggccagcc
     8701 gagtctcgcc acctcttcct cgggaacgcg gtggacacgg gcgatggcga gctgtgcggt
     8761 gagttcgggt tcgttccgcc cggcctccca gtgcgagacc gtgtggcggg tcctgaccat
     8821 cctggggccg aagccgagtt cgtcgtgggc cctggctatc agtctcgcgt agccgagctg
     8881 cgagaggccg agcgattccc gcgccacctt caacggatgc cgcgccatgt gcgagtcatc
     8941 gggtctctga tacatgccat acccatcggt agttgaaccg tcaccgaatc gtagagatcc
     9001 gcgcggcggg gtgataccga gtgcggcggg gtaaggcgca ccttgggcgc cacgaagtcc
     9061 cttgaggcgt ggcacggatc acgtttctct ggatcccgcg aagccgcccc cctccactcg
     9121 ctgcggaagc aacggcggcg cgccacctca tccgtcgggc agtcaggggt agccatggag
     9181 agccagttcg attcaactgt cgccgcctat gcgcagagca tgagcgcgtt gccgttccgt
     9241 gaacacgtcg aggcgcacag cctgctgacg gccatcgggc ctgtcgcggg tctgtccgta
     9301 ctggatctcg gctgcggtac ggggtcgtac acgcggtatt tccacaccgc cggtgccggg
     9361 cgggtcgtgg gcctggacgc gtcgctgtcc atggtcgaat ccgcccgcgg cgaggaggaa
     9421 cgggagaggc gcggcatcac gtacgtacac cgcgacgcca cacgtcccct gaaggacacg
     9481 gacgtcgatg gcttgaccgg cgccttcgac ctggtgacct ccgtctatgt cctgccgtac
     9541 gcgatgtcgg aggaggagct cgccgcgatg tgcgcgacgg cccgccggtc gctgtcgccg
     9601 gcgggcggcc ggttcgtcgc gatgacgctc aacccggact tcgccacgga ccccgagtgg
     9661 taccggcact atggcatgcg gctcaccacg gaccggcccg gcggggaggg cgccacggtc
     9721 cgtctgacgg cgtggatcag cgggcagacc atcgatgtcg aggcccgtcg ctggtcggcc
     9781 ggtgcgcatg aacgggcctt gtgcgcggca gggttcgaca gcgtcagctg ggtgcggccg
     9841 gaggtgtccg aggcgggccg gaggaggttc ggtgacgact tctggtccaa gtacctcgcc
     9901 tgcccgcatg cgttgatcat cgacgccacc gcggaaccgg cccccgacac ggactccggc
     9961 tccccctccg gccgcgaaac cgactgagga gccttccgcc atggaattcc tcgtacagcc
    10021 gtgcaccgcc gaaagccggc ggatcctggc gcgtgccgag cacctgctga tcgggctcag
    10081 cccgtggaac ggttactaca agccccgcag agtcgaggcc ctggtcgatt gggcctgcgc
    10141 gaacttcgcg tccgtggacg tcttcgtccc cggttacgag gcagcgcata ccctgaccgc
    10201 ggccggattc gagccgcggg aagcggtcca ccgcacccgg cgcgccatca gccagctccg
    10261 caaccccgcc cagcgcgccc tgcggcgtgc cgacatcccg aaccccggag cccggttcca
    10321 cagctggacg cagctggcca accgtccccc gtacacacgc atgctggagc gcgtgcacca
    10381 ggcgtaccgg accgatgaag cggtgcgccg cgcctgccgc cttaccgccc gcggggccgt
    10441 gctgcacgcc gccgagcgag agccgagtga gcgacagatc gaccaggcgg tcggctacgc
    10501 cctcgccgag ctgcctctcg ttctcgacgg accggcgatc ttcggaacgg agtcctcggt
    10561 gttcgtgtac caccgggaga tggatctcct cgatcccttc atcaccggcc gggccacccg
    10621 gctccgtccc gcctccgggc agggatacgc cgtggtgcgg ccggccgacg accgttgcgt
    10681 accgtcccac cgcttatgac agcgaaacgg agcctccagc ccatggacca gcccaccacc
    10741 gaccccgacc tgcctctgcg ctaccccttt ccgccgggcg agctcggcgc cccaccgctg
    10801 ctgctgggct gggcccgcaa gaaacagccg gtatgcccgg tcgtgctgcc cagcggcgac
    10861 cgcgtatgga tggtcacccg caaggacgac atcacgcgcg tactcaccga cccgggcttc
    10921 tcccgcgacc tcacctaccc cggtgcaccc cgcttcgtcg gcgacgactt cacctccgtc
    10981 ccgggcggga tcttcaacct cgacccaccc gaccacacac gggtccgcca ggtgatcaac
    11041 cgcttctaca cccgcagcgc cgtcgaacgc caccggccgg tgatcgaagg ccacgcggtc
    11101 cggttgctcg acgacatggc acacggcccg aacccggccg acctcatgcc cgcctacgcc
    11161 gccgcgctcc ccctgcacgt ggcccgcgaa atcctcgcgg tgccgccgga gttgcacagc
    11221 gactaccaca gctgtttccg cacccagacc cacctcgccg cctccgcggc ggcggtcgcg
    11281 gctgccaccg catcgatcac cgacttcacc agggaagtca tcgaaaccaa acgacgggac
    11341 ccggaccccg acgaacccat cggagcactg atacgggccc gatccggcgg cctcatcagc
    11401 gaaggcgaac tgatcggcac gacctcgtac ctgttcgtca ccggatccga acccctcacc
    11461 gccccgctcg gcaccggcgt tatcaccctc ctcacccacc cccggcaact ccgggagtgc
    11521 atcaaggctc cggacctgtg gcccaaagcc gtggaagaga tcctgcgcta ccaccacaac
    11581 ggcgtactcg gaatgcctcg gatcgccacc gaggacgtca cactgcacga cgtcaccatc
    11641 cgcaagggcg agggcgtctg ctgcccgatg ctcggagcca cccacgaccc cgcgcactac
    11701 cccaacccct cccgcttcaa catccaccgc accaccgacg cctcggccac cttcggcgcc
    11761 gggccccatt tctgcctcgg cgcccccctg acccgcctct tcctgcacac cgcctatcgg
    11821 gccctcttca cccgcttccc gtccctcttc ctcgccgtcc ccgaacgcga gatcccctgg
    11881 gaggacaacc ttctcttcat caaacccgcg agcctcccca tcgcctggtg acccgcaact
    11941 cacagagtca ccggtccagc caaggactgg ctggccggtc acgagcggag ccataggtga
    12001 atggcagcga cggttaccgt gccgtggaag acgtatgccc ttttgtcgaa tcgggtggct
    12061 atggcacgga agcccttgag ggcgttgatc gttcgctcga cttcgttgcg gcgttcgtag
    12121 atcgctttgt cgaagctggt gggccggccg ccggcgctgc cgcggcgctg gcggttggcc
    12181 cgctggtcct tgcgctcggg aatggtgtgc ttgatctacc gacgccgcag acaccggcgg
    12241 ttccgacggg acgagtacgc tttgtcgccg cccagatgat cggggcgcgt gcgcggatga
    12301 ccgccggccg gacgcgggac acggatgcgc tcgaggacgg ggatcagctg cggggcatcg
    12361 ccccaccggc ctggtgtgat gacgatcgcg agcggacgtc ggccgccctc accggccaaa
    12421 tggatcttgc aggtcagccc gccgcgggaa cgtcccaggg cctcatccga atgatgctga
    12481 acaggtcggc cgcgtcgtcc agggacacgc ggcgcggcgg tgcgggcgcc tgcggcgtgt
    12541 tgatgggcac ggcaggaggt cgagtccacg ctgaccacgc tccagtcaat gcggccttgc
    12601 gcgccggcat aggcctggac ggcccgcaga atccgatccc atgtgccgtc cgacgttcct
    12661 cggcaagtga ggccccagac gggcccattc ctcgttcgac agatcacccc gccccacgcc
    12721 gactggaacg atcgcaacgt aagccagtca cgtggaccca ccgggcaggc ccttgggctt
    12781 gtcgtcaaaa gatcttgccg agacggtgcg gaggtcgtgc atcatgcatg cgtggaacat
    12841 cttcctgtcg acgtgcgcgc ttcgcttcgg ctgttcgcct tctacctggc gaacggcacc
    12901 atcgaccttg agttgctcga cgggttcgac taccgctcaa ccgtcattca ctccggctca
    12961 tcgttggagc aggtcttcgc gatctacggc aatgtgctgc aaattgatgc aaacggcatg
    13021 gtgctgaatg acggggatgc gcagtaccgg gtcgcgcagt ggattcgggc gtgctgcgac
    13081 ccgagctatc gggtcgagcc gccgttcgaa gcctgggaga ccgaactcca tctctgaggg
    13141 ccaggcgcgc gtcgtcggga catcacgccc acatcaagag cgaggcgaga gcgacggctg
    13201 cctggtagga ctcggcggtc ttgtcatagc gggtggctag gccgcgtcac tgcctcagcc
    13261 ggttgaagca ccgctccacc atgttgcgcc gcttgtgcaa gtcccgatcg aacgccgacg
    13321 ggcggccgtc ggcgcttccc cgtcggaggc ggtttacgac ctgatcggcc cgctcgggaa
    13381 tggcgtgggc gatgcctcgc cttcgcagcc aggtgcggat cgcctcggag ctgtagccct
    13441 tgtcgcagat gacgtggtcc ggccgcatcc ggggtcgtcc cggcccgatt cgggggaccc
    13501 ggatcgcgtc cgtaacggcg gtgaactggg tgcagtcgtt ggcgttcccg cccgtcagca
    13561 cgaacgtgag cggacggcca gagccgtcgc aggccaggtg aattttgctg gtcagtccac
    13621 ctcgggaccg tccgagggca gggctccggg cccccctttt cgggctccgg cagcgtgctg
    13681 gtgggcgcgg acgatgctgg agtcgactga caccagccag tcgatgtcac cggccgcgtc
    13741 ggcctgcgct tgcgcggtct ggagcatccg ctcgaaggtg ccgtccgccg cccaccggcg
    13801 gaaacgggtg tggagactgg cccacgaccc gtatcgctcg ggcacatccc gccaggcgac
    13861 cccggtgcga aacctccaca cgatcccgtt gagcaccatc cggtcatcca gacgcggccg
    13921 cccagcacgg gcctgggcgg cagcggctgc acaagttccc actcgacatc ggacagttca
    13981 tgacggcata tcacctcacc atgatccaca acccaagatc gctttgacga cagaccctag
    14041 ggcggctcag tccgcgacgg gtgtcgcggg cacggcctgg ctcgccgccg acacgggtcc
    14101 tggcgcagga cccactgacc aacccaaaca ccactcccga taactcagag cgactggaag
    14161 cgggcggtcg caaactcgct tggccactcg caagctgtcg tgctctgcgt gactgagcgt
    14221 tctatctggt tctgaccgct cttccgtgat gcctaccgct agaggaggac gcgggctctg
    14281 agcagagcga atgaggctcg accgtacatc tgccgcttga tcgttttgat tctgttgacg
    14341 tgtccttcga cgatgccgga gctgtaggac agggtgaggc ctgccgtgac ggcgtggaga
    14401 tcctggcgga tggagtcggc gaagatgcca atggcgtcgg ggccgcccag ctcggccttc
    14461 tgaatccagt cccggagtag gtagccgcga cggtggcgaa gcatgttcgt gaactcccgc
    14521 gcgaggtcac aggccaaagc gatctctggg caagcgttcc ggacagtttc cagctgggcg
    14581 acgtccgcgg ggctgagcgt ctcctcgggt cgcatgatcc atgaggtgat gtcacgaggg
    14641 cggaaagtca ccgagcgggc agggaccgcg ataccctccc ggattgccat cacgtagcgg
    14701 ttcacaacgt ggtatccgcc ggtgtagcca agggcgagca actcgcgata gagctgcatc
    14761 gaactggtgc agcccgcctg gaagcggtgc tgcacgtgct cgatgaacgg gtcgaggacg
    14821 ccatgcccgc ggtcctgcgc cgaggcgagg agccctgcca ggtccttgtt ccggtagcgc
    14881 cggaccgtct tgcggtccag ctgcaggcgg cggccgatgg cgctgaggga catgccggtg
    14941 gcggccagct cgttgacctc cccatggcgt cgtcggatcc ggtccaggag cggggtctca
    15001 ggcgtggccg acggtgggtt ggaccctagt ccggctggtc gccgtaggca ctcatgatgc
    15061 cgtcggcagg tcttctcgac ggccgctgag aggttctgga ggaggtgcca gcgatctgcc
    15121 acctccagcg cgtccgggcc ggcctgcctg atggccttgg tgaatgccat caggcggtct
    15181 cggcagacga tctcggcgcc ggggtgctcc tggagccagg tggcgaccgt gctggtctct
    15241 cgatccggca gcacgtccag aggcctggag gactcgacgt cgacgaggac ggtgccgtag
    15301 gtccggccct tgcggaatgc gaactcgtcg ataccgagta tccgtggggc ccgggctggc
    15361 acgggctgag cggccaactg cctcaggagg gtcgtccgcc ggccatggac ccgcagcttc
    15421 gcgcagaggc gttggcccgg gcggccgccg agctccacgg cgaccgctct catcgccgac
    15481 cgggccaccg ttgaagtacg aagtcgtggc ttggtcagcc ccgccacctg ttccacgtat
    15541 gtccgacgtc ggcactggct ctgatcacag aagaatcgac gcacacggag acggatgatc
    15601 atccggtggc cgccgacggg cagcccagcg atgtgacgcc agtaccggct gtggacgcgt
    15661 gcggcgggat gctgacactg aggacatggt cctgggggtc cgtagccagc cgcgtcgatc
    15721 atcaaggtgt cgtccgcgac cacggcctgg cgcaccacga ggttcacgcc cgggaagagg
    15781 acgtcttcga gtgttcccaa cttcatgagg acgtcatccc ctggcggaac ggtcctgcag
    15841 tccagtcctt cgagcgccag ccgtcagggc aatggcagac ttcggaggtg aattctgatc
    15901 tttatcccga gctggccgct gcgggcagcc ttgccgccgc gctggaacaa ctggcggccg
    15961 accttggtgt cgatctcgtg gtggtgcccg gcggctgggg ggcactcgtt tcggcgggca
    16021 tcgctgcctc ggtccctgag cgcaagccgc tgtccgttca catcggggct gagagtcgct
    16081 ggttcggcgt ctccggttgg agccaggggg tcgaactcat caccggatcc acgcccgatc
    16141 tggcggatgt cgtcagggct ggcgtggcgt gggggcaggg ccggagcctg cgggagctgc
    16201 gggcggagct gccgtttctg cactccagcg agcgagcaga ggcgcacgac cgcgggccgg
    16261 ccgcagtcgt ggagctgcag tggcgtttga tgcgggaaca ggcagccgag gcgccggatt
    16321 tccctgagtt cggcgcactc gtggaagcgg ctcacgtcga acggcggctg cgagaactct
    16381 atgtgttctc cagccactgg acgctcggct tcagctcctg caccggattc ccgttcgaga
    16441 atgaggtcgc catcgccccg tcatatggcg gcggtccgta ccgcgtgctg aagcaccctc
    16501 acgccgacgt catcagcgag gccgcaacgg ccgaagaggc tgtcgccttg gcggtgaccc
    16561 gcctccccgc cggacttggc ccggcggtca cgggcaccgc caaatgaagt gagtggcgct
    16621 aggtgggggg gccgaagccc gtacccgtca tggacggtta gccatctcgc gaccatgtga
    16681 cttccggtta ggaggcgaca ggcggcggtc agcgtccgca tgagggaatg ttccccgcct
    16741 gggtggggaa ctacctgacg ccgaactggc cgaggtgggg aatggggcga ccgtcgtcat
    16801 cccaggtcgg gcggttggat cagggggccc agggcagtcg tacggcctcg atctccgagt
    16861 cgttgaggag ggcttcgacg agcggggctg ggccggcgac cttggtggcc cacaggtcgt
    16921 agtccgtgca caggacccag gaccggtcgt gggcccagag gttggacggg ctccaggagt
    16981 cttcttcggg gtggtcgtac agggccttcg cgtctgcgag ccggcctgcc cggacgtgca
    17041 ggttgtcgaa gtcctctgcc ccgagcacaa gcggggtgta gtaggccagg cagcgggtgt
    17101 ctgcgccctg cgggctgtgc tctgtgagga tctcgaccag tcggttccag tcggagcggt
    17161 ccaggctgcc ctcggacgga ccggtgatgc cgaccggcca cccgcccgac tcgcggagcg
    17221 aagggaagga tgtgtggcag ggcagacgcc cctcggcggc cacgggatcg ccggtccgcc
    17281 gggcgagctc tgcccagcgc aggcggcgat agcgtggtcc ggggtgctcg cctgtggact
    17341 tggcgaaggg gtccgagggg tcggtgaaca cgtccgcggg gtcgagaccc gggatgatct
    17401 cgggactggt gctgttcctg ttcaggaagg cctgcaggta ctcgacgtac gacatgtcga
    17461 agggcccgag ttcgtgttcg tacatggcat ggagtaccca ggccgcgtcg ggcagcgccg
    17521 gcggcatgaa cccggtgagc ccgtcctcgg cggccagttc cctcagccag tcggtttccg
    17581 tggacgcggc gagcggccag cgggcggttg ccagagcggc ggaggtcttg tacatcagct
    17641 caggatatga gcgcagaccc ggtgctgctc aggcgcctgc ccggtctgga cgcggctgtc
    17701 ctcccggcgt aggcatcacg gaagagcggt cagaaccaat cacaacgctc agtcacacgg
    17761 tttgacggcc gctgatagtt ggcgggccgg gtgaggaggt gtccaggtga gtggggtgac
    17821 ggccagtcgc aactggcgag ttcggtcgac gatggctggc gagccgctgg ccgtttgtat
    17881 cacgcagagt gagctcgctc ggcccgtcta ctgtgcgccg tgaggtcctc gccaaacggg
    17941 ccgggccatg gttacgcggc cccggtcttt cagttctgct gactgcgggc gtggcttctg
    18001 gatcggagct tggtgattgc cagcttcact cgttgaggtt taacggttat acggagtttg
    18061 gtggtttttc caggccgctt tgaggatggg ggcgggtgtt gccagtactg cggggtgggc
    18121 gatcaggttc tggaccatga tgaatcgccg gtagaggtcc ggattagctg gcaggcggtc
    18181 tacggtccgt cgtaccaggt ggtgcgcgag ggtggccggg ttgaggcggg tgtggttcca
    18241 tccggtgtcg gagtaggcag caatgttcca ggggacgcgg atcgtcgagg ggatggtgcg
    18301 ctggaaggtg cgtcccagct ctggtccgcg ccggtgctcg agagacggtc gagggtctgg
    18361 gcttggagtg cggcgatgtg gtcataccgt ggccgtagat ggggttgagg gtgcagacgg
    18421 cgtcgccgat ggcgattact gagtttttcg ttagttctgt gggtgcaggg gctgtagcgg
    18481 tgtaaagatg ttggtgtgcc ttccgagcct ccgcccgttg tgtccgaagc tcccgttccg
    18541 ccgctgacgc ggccgcagtt gactgaggcc cggcgtatgc gcgcggtcga gttgttcgag
    18601 cagggacggg ctgacgtcga ggtcgcacgg atggtgggga tccaccccga gagcgtgcgc
    18661 cggtggaagc gcacgtggga acagggcggg gctcaggcgc tgcggcgtcg gccggtcact
    18721 ggccggccgc ccaagctgga tgatgcccaa gtcgaggtgg ttcgggccgc gttggaacag
    18781 ggcgcccagg cgcacgggtt cgaggccgat ctgtggaccc tggagcgtgc tggcgtcgtg
    18841 attgaacggc tgaccggggt gacgctggcg cgcgcatcgg tgtggcgatt actgaccggg
    18901 cggctggggc ggagtctgca gcggcccaag caggcggtcg agcgggacga gtccgagatc
    18961 gccggtgggt cacccaggag tggccgcgta tcaaaaaggg gcggtgaaga aacgtgcatg
    19021 gatcgtgttc ttcgacgaat cgggcgtgtc cttgctgccc caggtccgtt gcacctacgc
    19081 gccccgcggc cgcactccca tcctgcggca ccggctgaac tggaaacgcg cctcgatggc
    19141 cgccgccctg ggctaccacg ccagcgaccc cgggcgtggt ccgcggctgt gcttccacct
    19201 caagcccggc agctacgaca ccacctccct gatcgaggcg ctggaacagg tcaaaacgtt
    19261 ctacgccggt gaaccggtgg tgctggtgtg ggacggactg tccgcccact ggagccggga
    19321 catgcgagcc tgggcggccg agcaggactg gctcactttg gaacggctgc ccgcctatgc
    19381 acccgaactc aacccggtgg aactgctgtg gtccgccatc aagacacgcg agctggccaa
    19441 cctcgcccgc gaccacctcg ccgaggtcgc ccgacgccac cgaacgcggc aaccagcgcg
    19501 tctgctccaa cgaacaactc ccctggtcct tcctcaccca caccggacta accattcacc
    19561 acaaaccacc acagaactaa cgaaaagctc agtagtcctc gccgtccctc tcgccgacgg
    19621 ccccaatgat ccgcccgtgc tcatcgcgcc ggatgtgcac tttgcgctgt ccgctggtga
    19681 gggcgcggtc ggcgagctgt tcgaccaggc gctcatcgtg gctgcgcagg gcttggagta
    19741 cggctacgag ggggcggtag gaggcggagg cgaccatgtc ggtcgggtcc tcgccgggct
    19801 ggaggaagac cggcacgatg atgcgggcga tcttcgtgct gccgtcgcgg ttgagtcgca
    19861 gtgcgcggcc gatgtcctgc acgactccac ctgggagccg cgggtgtcgg cgaagcagat
    19921 ggcttccacg cttcgctcgc cggtgatgtc cacgccctcc tcgagtacgc gcacggaggc
    19981 gaggaatgcc cggtgcacgc gccggttggt ggcgtcgatg ccgcctgcga actgccgcag
    20041 gacttcgcgc cgctccgtga cgagatggtc gccgcacagc catgcggacc agacgcggtc
    20101 cgggggtacg tggcggccgg tctcgagctc gtagaactcg gcgtcgatcg acgcgcgggg
    20161 agccggtccg cggcggcctc ctccaccttc tggtggaagg tcatcacggt gcgcaggttg
    20221 tgcgcggccg cgtgctccag gagcgccgtc tgcaagagcg ccaggcgcca gccccgcagg
    20281 gcctcgtccg actctccgaa gacgggggga ggggccgcgg atctccagga cgtcgatctc
    20341 gaacccggcg aggatgcccc gctcgatcag ctgcgtcgtc acctgcggga cgctgcccac
    20401 catcgagacc acggcaaccg gtaacccatt gctcgtcgtg cgcaggttcc tcaacaccgt
    20461 gctggtgcgc gcccccggcc ccgggctgcg cctgcgggcg gctgcctgat cccgacggtc
    20521 ggcgctgggt accagctccg accgcctcgg ccggtacgct gcccggggcg gcgccgctgg
    20581 gtgtcacccg ttcggcggaa aggcgggcag gggccgcttg gttctccgtc ctcctgggca
    20641 gcacatggcg tcaacgtcgc cctgtcgcac ggaggacccc tatgcccctt cctgactcg
//
