LOCUS       NZ_LNBE01000003        10870 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_3, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000003
VERSION     NZ_LNBE01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2630732)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 2630732)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2630732)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2255392
            Orig. end                         :: 2266262
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10870
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10870
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10870
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5870](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5870
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(333..1508)
                     /locus_tag="AS857_RS15440"
     CDS             complement(333..1508)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Trp_halogenase"
                     /gene_functions="biosynthetic-additional (halogenases)
                     conventional flavin-dependent halogenase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1119: halogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019888174.1"
                     /locus_tag="AS857_RS15440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptophan 7-halogenase"
                     /protein_id="WP_079110362.1"
                     /sec_met_domain="Trp_halogenase (E-value: 4.3e-06,
                     bitscore: 22.9, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPVRPPGQADAAVAGSGPAGAVAALVMARAGYRVLLLDDGGHS
                     SPRAGQAGFKIGESLPPAARPLLRDLGLLDGFLAAGHPRSTGIYSAWGSEELHGRSHLF
                     DPYGHGWHLDRLRFDAFLRDAAVAAGAELRRATVLDQLGRRLIVRERGTATVTELHCRW
                     TVDATGRRCVIGRRHGHRRRQDRLVTAYTVFRDRHPADQHREHREDVEARTLVEAAPHG
                     WWYTTRIPAGRLVAYLTDPDLADPSLRTPQGFWRGVARTGHVYRRLEGYDPASAPVPCW
                     APAHGLQLSPAAGPGWVAAGDAALAFDPLSSQGILTALHTGARAGQTVAACLADAGRTA
                     DELADYTEFLEGIAAAYRRHHAYAYDQEQRWPSSPFWRRRRTGVKALPGIA"
     gene            complement(1505..4543)
                     /locus_tag="AS857_RS15445"
     CDS             complement(1505..4543)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036537.4"
                     /locus_tag="AS857_RS15445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="WP_058043664.1"
                     /transl_table=11
                     /translation="MIGTPEPFDENAENAGQPQLPGCPENAAAALKQMFVDMTQGERIS
                     RGQDPVKRPVFLKPHGVARGVLTVSPDLPPELRLGFLQCAHERADGLTAWVRFSSDTVP
                     GVPDLKTTLGLGIKLFGVPGPKLLEGEDEAGTQDLLLQNHDVFFVDTAQDMCEFTRAGA
                     VDGNYEPYLQAHPITREILDAMAKPEESVLTATYWGVLPYAFGPDRFVKYKLVPAGCEP
                     GDPAAVPADEDPTYLGADLRHRLAAGTAAFDLMLQFRTDPQRMPLDRATVRWEESGSAP
                     VKVARLTLRKQDVTVRGQAAYGENLAYNPWHSLAEHRPVGSIAEVRKTVYRASAEQRRD
                     VNGIPATEPGPARPPVTPPPGRDTRIVRAAVHPAIGVARVGDSMDEYVLGPEVDHPAAQ
                     PTGSYKDATGALKRQAVRFRVYGYNAAGEPVAELTADNADLRWTVHVANVKAAWYQFQL
                     ALDIPEAAGTMDSNLRNPKVPAAQRKRLAINPGPRSVRGRNRSGKAEYRFDTGTFLERP
                     VYLGELRTDDSGRLVFLGGRGVSASVTDTPAGDFANNDGWYDDVSDGPVTAEVSIAGRS
                     IPVDPGWVVTAPPNYAPELTSVRTMYDLVRDAFVTAGLLPEPQQVSFTRDVLPVLRRLC
                     GLQWVNQGLAAQFGHGGREHFLDPDRLAKLADPSPVHQELRSQVWLSMRDYDRDGTSPV
                     PWPAVYGDAMNLPPQTVRQHVALSPLQYRLLERWAGGNFVADYDPQAVPPASLDDVPFS
                     QRPQTLDRAALSFCLADAFHPGCELTWPMRHTTLYSSPFRVRHRDPLAPKPPSYGLTLT
                     PKEALSFDGPLYAQGPGHLTRWMAVPWQTDTASCRSGYERLYGFGHYDPYLPTFWPARV
                     PNHVLAEDEYDIVVDTSRPMAERLAAFEKRRTWLRWLPTGNEATMNAMVTDFGKLGVVE
                     RRPGPTDDPRFPAEMLVESEVSFPREPAPPARRNLVTVHVPLGADPVHGPAAMATAVTM
                     ADLPDEEVSAGFIAKVKRFRPSP"
     gene            4673..4981
                     /locus_tag="AS857_RS39570"
     CDS             4673..4981
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS39570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_144440828.1"
                     /transl_table=11
                     /translation="MTNGETGRPNGGGSFVQTGHSMVIDLTGAPHIAEESGMAKYEDAA
                     QVVVTPASVVFSLTPEHREQAQRCLEKSGEVRISFSEVSVTNLTEIRELGGDGVLVD"
     gene            5001..5870
                     /locus_tag="AS857_RS15455"
     CDS             5001..5870
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /locus_tag="AS857_RS15455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_160330237.1"
                     /sec_met_domain="DUF692 (E-value: 4.4e-34, bitscore: 114.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSPAVPAGPGIGVAYSRTTPGLISRHPGLLDYVEVPFELLRHDP
                     TVAKALGGTPTVLHCASLSLAGSVPPTAAVMSSIEEWVGETRTPWVGEHLSFITAERAH
                     TDFSADVYAPGEPWNIGYTVSPPMNLETVESVVRSLERAASRFSVPLIIENPPVYFSMP
                     GSTMSQVEFISEVAARSQVGLLLDLAHFCITSRTVGYDPLAELARFPLDRVVEVHISGF
                     SEEAGGWWDNHASRAPDVELELLAMVVERSPVRAVTLEYNWSSRFPVGALLEEIARTRD
                     VIDACRRK"
     gene            5879..6745
                     /locus_tag="AS857_RS15460"
     CDS             5879..6745
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS15460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_144440829.1"
                     /transl_table=11
                     /translation="MNSVVARCLLEPGYLDRLARDPQRELTALPVDEETRAAIGTLDVG
                     QVRLFAGFVTKVQHNDLWDTFPRTRALLRYYGIELETFADYRAHYVEMCRAGKPSRAEK
                     AARFLRFLERRLRDSARANCPGLLDVLIHERLQWEVTQEVLGHRRSAPVRPAAREVRAG
                     SLDQRDQRVVVACDVIRVAALDHDPLEIAARLQDGGSALAHLEPKAVCFCYWGRTSEQT
                     VSVLTVDTATAAVLSAVDGHRSVDDVLDESRAVLPEAGRTHLARVLDGAVHQGLVLLVD
                     SPAREVG"
     gene            6748..8769
                     /locus_tag="AS857_RS15465"
     CDS             6748..8769
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /locus_tag="AS857_RS15465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="radical SAM protein"
                     /protein_id="WP_079110364.1"
                     /sec_met_domain="PF04055 (E-value: 9.8e-16, bitscore: 55.7,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVTFVDNLLLEERPEGYHFDLQPHLGLISLLAVVHQAGHEGTLV
                     DPKLGVSSGRLALDASLYERIADDVLASRPDAVGMTSLGCNFMATAKVAAHLKRLAPDL
                     PILLGGPHASILDVPVLQRYPQFDLVVRGEAELTLPPLLDRLHSRRFSGLLGITHRNGH
                     DIHRAPDAPLVTDLDALPPASYDHYPLAQAGLTTIRVEAGRGCPFACTFCSTASFFGRR
                     YRLKSAERLCADLDRLHDAYGITHFALTHDLFTVNKGKVREFCEEVAPRGYTWTCSARM
                     DCVDSDLLRAMGQAGCRSIYYGVETGSPRMQKEVAKRLDLGLFWPTLGATREAGMEAVA
                     SFITGYPQETEADQAETLDLIGAAWIRQPVMLATQLHLLTPEPGTELLDRYRDRLAYDG
                     HISDFNLPTLEADDAEITRLDPEVFVNHHYYRSVLPRHRHILVTSAYRVLCRLGRAVQR
                     HLLSRYDNSLSRFFDDLISWERTAPNAPSDTAELVQAYLCARWGAEDYLLSLVRYMLAA
                     TDPARGRYGDGPDRPDAPSRSADPLVQTSPRAAVLRDIHACPEILDLLTSATTPEPVKV
                     PHSLRTRRGHGLLLPGSDGQTMRYMVMSEDSLHLLAFLEAPRSRAEIVRYLGVRGAEAE
                     PLDQLLDHLLALGVLRQREPAEREEHGEYDEPRETARV"
     gene            8771..9898
                     /locus_tag="AS857_RS15470"
     CDS             8771..9898
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /locus_tag="AS857_RS15470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_144440830.1"
                     /transl_table=11
                     /translation="MTTRRDAMDVIEGFYCSQTVWALHRLGVLPRLEDGADVPALAEEF
                     ALDEQLLSAVLDFIHRTTDLLVREDHHYRLAPSCRPYRRTAFHLEKFLGAYGPVIAGTA
                     TVLAAPGSGPELRDLPSLARAFGQLEPRSPSLTARVLRAWGVTSLFDLGCGAATLLVEL
                     ATADPEFRGWGADSSDAMVKAADERISRSGLGGRLRILRSDVRELGGLTPRPDVEDVQA
                     LYGRSIANEFFADGGRSAVEVLTGLKELFPGRLLFLEDYYGRLTHHPPAGGNHRHTLLQ
                     DLAQVLSGQGVPPPDLPGWSGVYQAAGCSLVKAYEGHNEEISWFIHVLVLGRRPRPGPP
                     SPRAMSRDRRHQAATVQPGGDGHDLPRCHGAAPGS"
     misc_feature    10373..10382
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    10516..10530
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.77"
                     /tool="antismash"
ORIGIN
        1 tatcggccgg cgaccggctc accgaaatct cgccgcgcac ggtgactccg ggcgcggaat
       61 cgcaactgag gtagagccgg accaccgtgg acttacccgc gatcaagggt acggaatggt
      121 tcaggtcctg cactgtctgg gtgacttcga ctgcatccag gttcacgtca ggcgccatga
      181 tcgacctcct catagcgaca tgaatcgggc gacatgaatc ctctgttcca ccctacggcc
      241 ggtcgggtga ccctgccgcg cgagcggatc gcagacgctg ccggaacgag tggtttcgct
      301 taggcgcgtg tagatgcgtg ttccggcccc tttcaggcaa tgccgggcag ggccttcact
      361 cccgtgcgac gtcgtcgcca gaaggggctg gagggccagc gttgctcctg gtcgtacgcg
      421 tacgcatgat gtctgcggta ggcagccgcg atgccctcca ggaactctgt gtagtcggca
      481 agctcatcgg ccgtccggcc cgcgtcggcc agacacgcgg ccacggtctg tccggcacgg
      541 gcaccggtgt gcagcgccgt gaggatgccc tgcgaagaga gcgggtcgaa ggcgagggcc
      601 gcatccccgg ccgccaccca gcccgggcct gcggcggggg acagctgcag gccgtgggcg
      661 ggtgcccagc acggcacggg agcagaggcg gggtcgtagc cctccagccg gcggtacacg
      721 tggccggttc gggcaactcc gcgccagaag ccctgggggg tacggaggga ggggtcggct
      781 aggtcggggt cggtcagata ggcgaccagg cgtccggccg ggattcgggt ggtgtaccac
      841 cagccgtggg gcgccgcctc gacaagggtg cgcgcctcaa cgtcctcccg gtgctcccgg
      901 tgctggtccg cggggtgacg gtcgcggaac acggtgtacg cggtgacgag ccggtcctgc
      961 cggcggcgat ggccgtgccg gcggccgatg acgcagcgcc gaccagtggc atcgaccgtc
     1021 cagcgacagt gcaactccgt cacggtggcg gtgcctcgct cacggacgat caggcgcctg
     1081 ccgagctggt ccagcaccgt ggcccgccgc agttcggcgc ctgccgcgac ggccgcgtcc
     1141 cgcaggaagg cgtcgaagcg gagtctgtcg aggtgccagc cgtgcccgta cgggtcgaac
     1201 aggtggctgc ggccgtgcag ctcctctgaa ccccaagcac tgtagattcc cgtggacctc
     1261 ggatgcccgg cggccagaaa gccgtccagc agcccgaggt cgcgcaacag cgggcgggcg
     1321 gcgggtggca gggactcccc gatcttgaac ccggcttggc ccgcgcgcgg cgaggaatgc
     1381 ccgccatcgt cgaggagcag gacccggtag ccggccctcg ccatgaccag tgccgctacg
     1441 gcgcccgccg ggccgctgcc tgccacagcc gcgtcggcct gtccgggcgg gcgcacgggc
     1501 gcggtcacgg actcggccgg aagcgtttga ctttcgcgat gaagcctgcc gaaacctcct
     1561 cgtcgggcag gtctgccatg gtgactgcag tggccatggc ggccgggccg tggaccgggt
     1621 cggcgccgag gggaacgtgc accgtgacca ggttgcgccg ggccggcggg gcgggctcac
     1681 gcgggaagct gacctcggac tcgaccagca tctccgccgg gaagcgggga tcgtcggtgg
     1741 gtccgggacg ccgctcgacg acaccgagct tgccgaagtc cgtcaccatg gcgttcatgg
     1801 ttgcctcgtt tcccgtgggg agccagcgga gccaggtccg ccgcttctcg aacgcggcca
     1861 ggcgctccgc catggggcgg gaggtgtcga cgacgatgtc gtactcatcc tcggcgagca
     1921 cgtggttggg cactcgggcc ggccagaaag tggggaggta cgggtcgtag tggccaaatc
     1981 cgtagagtcg ttcgtagccg gacctgcaac ttgcggtgtc ggtctgccag ggcacggcca
     2041 tccaccgggt caggtggccg gggccctggg cgtagagcgg gccgtcgaag gagagcgcct
     2101 ccttcggggt gagtgtcagg ccgtaggacg gcggcttcgg cgcgagcgga tcgcggtggc
     2161 gtacccggaa cgggctgctg tacagggtgg tgtggcgcat cggccaggtg agctcgcagc
     2221 cggggtggaa ggcgtcggcg aggcagaacg acagggccgc ccggtcgagg gtctggggcc
     2281 tctgggagaa cgggacgtcg tcgagggagg cgggtggcac ggcctgcggg tcgtagtcgg
     2341 cgacgaagtt tccgccggcc cagcgctcca ggaggcggta ctggagcggg gagagcgcca
     2401 cgtgctggcg tacggtctgg ggcggtaggt tcatcgcgtc gccgtagacc gccggccacg
     2461 gcacgggcga cgtgccgtca cgatcgtagt cgcgcatcga gagccagacc tgggagcgca
     2521 gctcctggtg gacgggggag ggatccgcca gcttggcgag ccggtcgggg tcgaggaagt
     2581 gctcgcgtcc gccgtgcccg aactgggcgg ccagcccctg gttgacccac tgaagcccgc
     2641 acagccgacg cagtacgggc agcacgtcac gggtgaacga gacctgctgc ggttcgggga
     2701 ggagcccggc ggtgacgaag gcgtcgcgca ccaggtcgta catcgtgcgc accgaggtga
     2761 gttcgggtgc gtagttgggc ggcgcggtca ccacccagcc cgggtcgacc gggatggacc
     2821 gccccgcgat gctgacttcc gcggtgaccg ggccgtccga gacgtcgtcg taccagccgt
     2881 cgttgttggc gaagtcgccg gcgggagtat cggtgaccga ggccgagaca ccccggccgc
     2941 ccaggaagac cagccgaccg ctgtcgtcgg tgcgcagttc gcccaggtag acgggtcgtt
     3001 ccaggaaggt gccggtgtcg aagcggtatt cggccttgcc ggagcggttg cggccgcgca
     3061 ccgatcgggg cccgggattg atggccaggc gtttgcgctg ggcggccggg accttggggt
     3121 tgcgcaggtt gctgtccatg gtgccggccg cctccgggat gtcgagggcc agctggaact
     3181 ggtaccaggc ggccttcacg ttggcgacgt ggacggtcca gcgcaggtcc gcgttgtcgg
     3241 cggtcagctc ggcgaccggc tcgccggcgg cgttgtagcc gtagacccgg aagcggacgg
     3301 cctggcgctt cagggcgccg gtggcatcct tgtagctccc ggtgggctgc gcggccggat
     3361 ggtcgacctc ggggccgagg acgtactcgt ccatgctgtc gccgacccgg gccactccga
     3421 tcgcggggtg cacggccgcc ctgacgatcc tggtgtcgcg tccgggcggc ggcgtgaccg
     3481 gcgggcgggc ggggccgggc tcggtcgccg ggatgccgtt cacgtcgcgg cgttgttcgg
     3541 ccgaggcgcg gtagacggtc ttgcgcactt cggcaatgct gcccaccggg cggtgctcgg
     3601 cgaggctgtg ccacgggttg taagcgaggt tctcgccgta ggcggcctgg ccccggacgg
     3661 tgacgtcctg ctttcggagg gtcagccggg ccactttcac cggcgcactc ccggactcct
     3721 cccagcgcac ggtggcccgg tccagcggca tgcgctgcgg gtcggtgcgg aactggagca
     3781 tcaggtcgaa ggccgcagtt cccgccgcga gccggtgtcg caggtcggcg ccgagatacg
     3841 tgggatcctc gtcggccggc accgccgctg ggtcgccggg ctcacaacct gcgggaacga
     3901 gtttgtactt gacgaagcgg tcagggccga aggcgtaggg cagcacgccc cagtacgtgg
     3961 ccgtcagcac gctctcctcg ggcttggcca tcgcgtcgag gatttcccgg gtgatcgggt
     4021 gagcctgcag gtacggctcg tagttgccgt cgacggcacc ggccctggtg aactcgcaca
     4081 tgtcctgggc cgtgtcgacg aagaagacgt catggttctg cagcagcagg tcctgggtgc
     4141 cggcctcgtc ctcgccttcc agcagcttcg ggccgggcac tccgaacagc ttgatgccga
     4201 gaccgagggt ggtcttcaga tcgggaacgc cgggaacggt gtcgctggag aaacgtaccc
     4261 aggcggtcag cccgtcagcc cgttcatggg cgcattgcag gaacccgagg cgcagttcgg
     4321 gtggcagatc gggggagacc gtgagcactc cacgggccac accgtgcggt ttgaggaata
     4381 ccggccgttt gacggggtcc tggccacggg atattcgctc gccctgggtc atgtcgacga
     4441 acatctgctt cagtgcggcg gccgcattct cggggcaccc tgggagctgc ggctgccctg
     4501 cgttctcggc gttctcgtcg aagggttcgg gggtcccgat catgctgttc tcccgtttag
     4561 tgcagcagac tgcggccgcg gtggccccga tccagtggtc cttgggacgc ccgcttccgt
     4621 ccactcgccc ccgatccagc gtgcacgaac ctttgcggac cagcacgccg aagtgaccaa
     4681 cggtgagacc ggccgtccga atggaggagg ttcattcgtc cagacgggtc attcgatggt
     4741 gattgacttg acaggtgcgc cgcatatcgc cgaagaatca ggtatggcca aatacgaaga
     4801 cgcggcacag gtggtcgtca ctccggcgtc cgtggtgttc tcactcacgc ccgagcatcg
     4861 tgaacaggca cagcggtgcc tggaaaagtc gggtgaggtg cggatctcgt tcagcgaggt
     4921 ctccgtgacc aacctgaccg agattcgcga actgggcgga gacggcgtcc tcgtcgactg
     4981 aacccggaac agcgaaaatc gtgccgagcc ctgcagttcc ggccggcccc ggaatcggag
     5041 tcgcgtacag ccgtaccacc ccaggcctca tcagccggca ccccggactt ctggactacg
     5101 tggaggtccc tttcgagctg ctccgtcatg acccgacggt cgcgaaggca ctgggtggga
     5161 ctcctacggt gctgcactgc gccagcctct ccctcgcagg ttcggtgcca ccgacggcag
     5221 ccgtcatgag cagcatcgag gagtgggtcg gggaaacccg gaccccatgg gtcggcgaac
     5281 acctttcctt catcacggcc gagcgggcgc acaccgactt ctcggccgat gtctatgccc
     5341 cgggtgaacc ctggaacatc ggatacacgg tcagccctcc gatgaacctc gagaccgttg
     5401 aatcggtggt ccggtcgctg gagcgcgcgg cgtccagatt cagtgtcccg ttgatcattg
     5461 agaacccgcc tgtttatttt tccatgcccg gctccaccat gagccaggtc gaattcatct
     5521 cagaggtggc tgcccgctcg caagtcggcc tgcttctcga tctcgcgcac ttctgcatca
     5581 cgtcccggac agttgggtac gacccgctgg ccgagctggc ccggtttccc ctggaccgag
     5641 tggtggaggt acatatctcg gggttctccg aagaggcggg agggtggtgg gacaatcacg
     5701 cgagccgggc gccggacgtg gagctggagt tgctcgcgat ggttgtcgag cgttcgcccg
     5761 tacgtgctgt gacgctggag tacaactggt cttctcgttt ccccgtgggc gctctgctgg
     5821 aagagatcgc ccgcacccgg gatgtgatcg acgcgtgccg acggaagtga gcagggccgt
     5881 gaactcggtc gtcgcccggt gcctgctgga acccgggtat ctcgaccggc tcgcacggga
     5941 cccgcaaagg gaattgacag cgctgccggt cgacgaggag acccgggccg ccatcggaac
     6001 gctcgatgtc gggcaggtgc gtctgttcgc cggcttcgtc accaaggtcc agcacaatga
     6061 cctgtgggac acctttccgc ggacacgggc cctgctcagg tattacggca tcgaactcga
     6121 gacgttcgcg gactaccgcg ctcactatgt ggaaatgtgc cgggccggca agccttcccg
     6181 ggcagagaag gcggcacgct tcctgaggtt tctggagcgc cgtctccggg attcggcgag
     6241 ggcgaactgc cccggccttc tggatgtcct gatccatgag cgtctgcagt gggaggtgac
     6301 acaggaggtt ctcgggcacc gtcgctccgc cccggttcgg ccggctgccc gtgaggtccg
     6361 tgccggctca ctcgatcagc gcgatcagcg cgtggtcgtc gcctgtgatg tcatccgcgt
     6421 cgccgcactg gaccacgacc cgctggagat tgcggcccgc ctccaggacg gtggcagcgc
     6481 tctcgcacac ctcgagccga aggccgtctg tttctgctac tggggacgca cgagcgagca
     6541 aacggtctcc gttctcacgg tggacacggc aaccgccgct gttctgtccg ctgtcgacgg
     6601 ccaccgatcg gtcgacgacg tccttgacga gagccgggcc gtcctgccgg aggccggtcg
     6661 cacccatctg gcccgcgtcc ttgacggtgc ggtgcaccaa gggctcgtgc tgctggtcga
     6721 cagcccggcg agagaagtgg ggtagagatg cgggtcacct ttgtcgacaa ccttctgctg
     6781 gaggagagac ccgaggggta ccacttcgac ctccagcccc atctcgggct catctccctg
     6841 cttgccgttg tgcaccaagc agggcatgaa gggactctgg tcgatcccaa actgggcgtc
     6901 agttcaggca ggctggcgct ggacgcatct ttgtacgagc gcatcgccga cgacgtcctc
     6961 gcctcacggc cggacgccgt cggaatgaca agcctcggct gcaatttcat ggccacggcg
     7021 aaagtcgcgg cgcacctcaa acgcctggcc cccgacctgc cgattctcct gggcggcccg
     7081 cacgcgagca tcctcgacgt gccggtgctc cagcgctacc cgcagttcga cctggtcgta
     7141 cgcggagagg ccgaactcac cctgccgcct ctcctcgaca gactccactc ccggcggttc
     7201 tccggtctgc tcggcatcac ccaccgcaac gggcacgaca ttcaccgggc ccccgacgcg
     7261 cccctggtca ccgacctcga cgcactcccg cccgcctcct acgaccacta ccccctggca
     7321 caggcagggc tgacgactat ccgggtggag gcgggccgcg gatgtccctt cgcctgcacc
     7381 ttctgctcga cagcgagctt cttcggccgc cgctaccggc tgaagtccgc ggaaaggctc
     7441 tgcgccgacc tggaccgctt gcacgacgcc tacggaatca cgcacttcgc actgacccac
     7501 gacctcttca cggtgaacaa ggggaaagtc cgggaattct gcgaagaggt ggcgccccga
     7561 ggctacactt ggacctgctc cgcacggatg gactgtgtcg acagcgacct cctccgggcc
     7621 atgggacagg ccggctgccg ctccatctac tacggcgtcg agaccgggtc cccgcgtatg
     7681 cagaaggaag tagccaagcg cctcgacctc ggcctgttct ggccgacact cggcgcgacc
     7741 cgcgaggccg gaatggaagc ggtggcctcc ttcatcacgg ggtacccgca ggaaaccgaa
     7801 gccgaccagg cggaaaccct ggacctcatc ggggcggcat ggatccggca acccgtaatg
     7861 ctcgccactc agttgcacct cctcacgccg gaaccgggaa ccgaactgct cgaccggtac
     7921 cgggaccgac tggcctacga cggccacatc tccgacttca acctcccgac ccttgaggcg
     7981 gacgacgcgg agatcacccg gctggacccc gaagtcttcg tcaaccacca ctactaccgg
     8041 agcgtcctgc ccaggcatcg ccacatcctc gtcacgtccg cctaccgggt cctgtgcaga
     8101 ctcggacgcg cagtgcaacg ccacctgctg tcgcgatacg acaacagcct cagcaggttc
     8161 ttcgacgact tgatctcctg ggagcgcacc gcaccgaacg ccccttcgga tacggcagag
     8221 ctggtacagg cgtacctctg cgcccggtgg ggcgcggagg actacctgct ctccctggtg
     8281 cgctacatgc tcgcagccac cgatccggcg cggggacgtt acggtgacgg accggaccga
     8341 ccggatgcgc cctcccgctc ggccgacccg ctggtgcaga cctctcctcg ggccgccgtg
     8401 ctgagggaca ttcacgcgtg ccccgaaatc ctggacctgc tgacctcggc cacgaccccg
     8461 gaaccggtga aggttccgca ctcgctgcgc acgcggcggg gccacggcct cctgctcccg
     8521 ggcagcgacg gacaaaccat gcggtacatg gtgatgagcg aggactccct gcatctcctc
     8581 gcgttcctcg aggcaccgcg aagcagagcg gagatcgtgc ggtacctcgg tgttcggggc
     8641 gccgaagcgg agccgctcga ccagcttctc gatcatctgc tcgctctcgg cgtcctcagg
     8701 cagagggaac ccgccgagcg cgaggaacac ggcgagtacg acgaaccccg tgagacggcc
     8761 cgtgtctgac atgacgacgc gacgggacgc catggacgtg atcgaggggt tctactgttc
     8821 ccagacggta tgggcactgc acaggctcgg cgtgctcccc cgcttggagg acggtgccga
     8881 cgtccccgcc cttgccgaag agttcgctct cgacgagcag cttctgtccg cggtgctcga
     8941 cttcatccac cggaccacgg acctgctggt ccgggaggat caccactacc gcctggctcc
     9001 gtcctgccgc ccttaccgcc gcaccgcgtt tcacctggag aagttcctcg gcgcgtatgg
     9061 cccagtcatc gccggcaccg ccacggtcct cgcagctccg gggagcggcc cggagctgag
     9121 ggacctcccc tccctggcac gagccttcgg acagctggaa ccacgctccc cctctttgac
     9181 cgcccgggtg ctgagggcct ggggcgtgac ctccctgttc gacctgggct gcggcgcagc
     9241 cacccttctc gtcgaactcg ccacggcaga cccggagttc cgtggatggg gcgccgactc
     9301 ctcggacgcc atggtgaagg ccgccgacga acgcatcagc cgttccggac tcggcggccg
     9361 cctccgcatc ctcagaagcg atgtccggga actcggaggg ctgaccccgc gcccggacgt
     9421 ggaggacgtc caagcgctct acggacgcag catcgcgaac gagttcttcg ccgatggcgg
     9481 ccggagcgcc gtcgaggtgc tgaccgggct caaagagctg ttcccgggcc gtctgctctt
     9541 cctcgaggac tactacggcc gcctcaccca ccacccgccc gccggtggga atcaccggca
     9601 caccctgctg caggacctcg ctcaggtgct cagcgggcag ggagtgccac ccccggatct
     9661 gcccggctgg tcgggtgtct atcaggccgc cggctgcagc ttggtcaagg cgtacgaagg
     9721 gcacaacgag gagatctcct ggttcatcca tgtcctggtt ctcggccgtc ggccccggcc
     9781 cggccctccg tcgccccgtg cgatgtcaag ggaccgccgc catcaggccg caacggtgca
     9841 gccaggtggc gatggtcatg acttgcccag gtgccatggt gcggctccag gctcctaagg
     9901 tctcggccag cgtgtactcg cccgacaaag catctgtgag cggggcgact gcgagtccct
     9961 cgacgtaggc cacgggtctg gccagggtgg gatgggtgag tgcggggcgg aacgacacct
    10021 ggttgcccac gacgcagggg gtgctcacca gcttggtggc gggcgagagg cgcacgcgcc
    10081 ggtgtatcag gtccgccagg tccatggggg aggtgattgc gggtccttgc gtcgggagcg
    10141 ggcgggcgcg gtggcgccag aaagcattgc tccggtgggt ccggtgttcc gcgtagtacc
    10201 caccggccca cttcctgtgc tgctcgacgg agtgccgctg accgtcgcgg tagaaggtca
    10261 gcgcggcggt cctgtcgccg tcctcggaca gcagagtgtg cacggtgacg gcaccggcca
    10321 tggcggtctg cagggccttc tccacgccgg acgaggagag cggatcgatg gtgaaggcgg
    10381 cctcaccgag tctgatgtag tcctcaccga caggatcggg gtcgaagtag ctggtggcgc
    10441 cgcagacctc caccgtggtc ccgggtccgt ctcccacgag cccgcggaac agttcggagc
    10501 cggccagcag gttctggaag aaaccgtcca gagatgactt gcgaacgccc gaacggtgca
    10561 ggtgctccag gtcggtgaag gccatggccc ggtagcttcc gcgcggcagg agcgtgcccc
    10621 acagccagcc gttcggcccg gcctccacgc gggttgccgg ggcgtcgcgc gggctgagac
    10681 gcaaggctgc gtgcagggcg atcgtccgag ggccggtgcg ctgcacgcgg ccgccgagga
    10741 cacgtgcccg gccggtggcg tcgaccagga atcgggcgcg aaggagctga ggcccctcgg
    10801 gtgtcgtgac cggcacctcc cagccgccgt tccctcggcg cggacggcgt gcgagggcgg
    10861 gctggagcac
//
