LOCUS       NZ_LNBE01000003        22505 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_3, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000003
VERSION     NZ_LNBE01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2630732)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 2630732)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2630732)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1991505
            Orig. end                         :: 2014010
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22505
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="19"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..22505
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22505
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:12505](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12505
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            complement(824..2068)
                     /locus_tag="AS857_RS14365"
     CDS             complement(824..2068)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262556.1"
                     /locus_tag="AS857_RS14365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="WP_079110325.1"
                     /transl_table=11
                     /translation="MNDRLDDISDIPETPASTPATDWTTTPVTTPITADLLRGALDLEY
                     TEHGVLPHRLPARARAQCADGQLAVAEAQPSGVRLVFRTRAGVIELDALRTKNVYKGAP
                     PRPDGVYDLLVDGRLAGRASVSGGNVLLVDMTTGSAETRPGPVGTVRFSGLPDRVKDVE
                     IWLPHNEITQLVALRTDAPVEPLPDGGRKKWLHHGSSISHGSDAASPSTTWPALAGFLG
                     DVELTNLGLGGSALLDPFTARALRDTPADVISIKVGINLVNTDLMRLRAFGPAVHGFLD
                     TVREGHPTTPLLVVSPILCPIHEDTPGPSAPDFSSLSEGKLRFRAAGDPAERAAGKLTL
                     NVIRDELARLVEQRAADDPNLHYLDGRELYGEADFAELPLPDQLHPDAAAHRRIGERFA
                     ALAFGSGGPFATTRV"
     misc_feature    2098..2113
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.55"
                     /tool="antismash"
     misc_feature    2175..2189
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.03"
                     /tool="antismash"
     misc_feature    complement(3109..3123)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            3128..4375
                     /locus_tag="AS857_RS14370"
     CDS             3128..4375
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS14370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_144440813.1"
                     /transl_table=11
                     /translation="MARVVVLGEFRADRIVPPFRDGGAKVTVLGFADLTSFLGPGTACG
                     ELPTTLDERRTLQLLEEHRAEVAVPNMGCPGQEQLLPVYSRAASRLRGAGCRMPVHPEG
                     FATSASDKVAFHRLAGERGWPVPRGVVCEAAAELPGAVGELGFPVLVKEARSEFHAGRH
                     YVRDREHLDRVSGEVRYPVLVQEAVSGEEYTLELLSGPRSSAAGPSATVAWPVASLGRL
                     GSDCAPGRRVRVAPAVLPARVQEELAATVVDMVESTAPLGPWQMDFAVTDDGRLQVIEL
                     NGRLGGVSNMSWASTGLDPHVAYAHAVLGRAPSTRRARAHRVALELPVRNGAALPPPPT
                     GTELMAFPGNPANRGPFIGGYHRPVLCVPGDRADVVSAWLRSLPPGTLLNGADEAAAQL
                     GRGLYALRRDGGRPPL"
     gene            complement(4459..5094)
                     /locus_tag="AS857_RS14375"
     CDS             complement(4459..5094)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS14375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058043486.1"
                     /transl_table=11
                     /translation="MIHMTETLAVQTVEDFLTADETAELTKVLDEHLAATGWVPVRPSE
                     GTTPPDAAQALLNGAVRRALPALRRVFPSASGTDPWLYHDLKPGDKIRPHVHGVGDPQA
                     RPQRIARVAFNLQDAERGGEFHLDTYSADALWSDTTPAAESDYAPGTRLVHEITARSGP
                     VKLDDLPHTRWICSPPPGTTVVYGTQLVHGVLPVTEGRVRKLITNLLA"
     gene            5276..6766
                     /locus_tag="AS857_RS14380"
     CDS             5276..6766
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026958.1"
                     /locus_tag="AS857_RS14380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_058043487.1"
                     /transl_table=11
                     /translation="MDTEVLVVGAGPTGLLLACELALAGVRVRVVDKRASPHRESRALN
                     LHPRSIELMDMRGLAGRFLAAGRTVPGWQFAVSLKRRLDFTGLDTRHPYALLLAQARTE
                     ALLAERARELGVEVHRGCEAVAVGQDAHGAVAGLSGPGGPVTIRCAYVVGCDGGRSRVR
                     QAAGIGFPGTRESMSAVVGDFAVADHACNERARQHGVLVARLESGLTRFVVMDPRRIRT
                     PSSEPVTLEEFRTSLRQVCGTDCGIGEPRWLSRFGNTTRLAGTYRRGRVLLAGDAAHVH
                     FPVGAQGLNTGLQDAMNLGWKLAAAVRGWAPPGLLDTYHAERHPVGHAVTAGTRAQTLL
                     VELPLTEQYGLPAELLCELLDTLLGMSEVNRYLAGRISALDTAYPPSLPDADPLTGRRM
                     PDIDLTTAAGPARVYELLRDGRFVLLHFGEGTGPDESSRTRAVTARRHEARSDLDGVSA
                     VLVRPDGHIAWATRTTDPRALNAERAEALLAWTGTRVG"
     gene            complement(6827..7516)
                     /locus_tag="AS857_RS14385"
     CDS             complement(6827..7516)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS14385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058043488.1"
                     /transl_table=11
                     /translation="MTTTHRPRRARRPRRPRTATIHAALGALLLGALCTTAGPGTTPAS
                     AAPARGTARTAPAVTAVTGAAPAGTALAGTALSCSLQTAPGDPLVFRPPVTGRVQRVSA
                     TGTVLLDRCTSADGSQYGIRSGRMVLRGAATANCLTAARPEGSAVITWYHGWAQRGRTA
                     GTSHLRPSSRGGGGYTPADAFLSGDVVSGRMADRHVRGWAVPTTDVTGCAARGLRTVAG
                     RGNISFS"
     gene            complement(7513..9762)
                     /locus_tag="AS857_RS14390"
     CDS             complement(7513..9762)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035: MMPL domain-containing transport protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /locus_tag="AS857_RS14390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MMPL family transporter"
                     /protein_id="WP_160330230.1"
                     /transl_table=11
                     /translation="MVSRPWRVLAGAGLLGAVLGLWLLGARPQFSGGGYLATGTEAARA
                     DAVLAERFHAGLPDLVLRADARSPAGSEDVSRAGLDLTVRLSRQPGVERVYSYWNTGAS
                     ELLSENGRSALVVADLAGDDTATARAAARLVPRFTGAHGPLRVSATGTSYVSAQATEVS
                     GQELLRAELLGVPLAALALLLAFGSPVAALLPVLTGLFAVLGAYPLLCALAGVMPVSAL
                     ATNVATALGFGVAVDYGLLLVSRYRQELAAVEGDAHDADGRPDTRGATLRTMRTAGRTV
                     FFSSGTIAVCLTPMLLFPLPFLRSLACAGIAVVLCCGLATAVVLPALLVLLGRWIDRGD
                     PLARWRRARSAESPLWRRVARAATGRPVLAGGCCVLLLAASALPFAHVRFGVTDYRVLP
                     AGLESHAVAAGIERDFSLPWDRAVTVLLPATDAVEQEAEVTAYARQLSERPDVASVVTG
                     LGTYAHGRQVAGPTPGSLLQITMGGTWLAVTGSGPPASDPDLVQTLRALPSPGPHLVTG
                     CPARVLDTKTALAHVLPFIGALLIGCVLVLLFLFTGSLLIPLKAVVLATLSMCASFGAM
                     VHVFQDGHLRGLLGSFAVTGELETAMPVVMFCFAFGVSVDYELFLIARIQEEYRAGADC
                     RTAVVNGIARTGRVITTASLLVAIAVLPLLTSHVVLLKMFGCGIALAVLVDATVVRGVL
                     VPAFMQLAGRANWWAPAPLRRLHRRLGLHTAFVPPAPTGAVPAQQKQTVRKKIRT"
     gene            10001..12505
                     /locus_tag="AS857_RS14395"
     CDS             10001..12505
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /locus_tag="AS857_RS14395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_058043490.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.3e-46, bitscore:
                     156.1, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKHAAQAPPSPSATDSGESVREDLPHPDRPVERWSVNASVRTAG
                     GEEHSLSAQFLCRRPGAGGGTGTARPGGGADRPAGHTVIWSHTDHATGGRTTESWLDEA
                     ALAEARSAFEDASAWDPHVRRAVLDALAGGRPLLPDRLLHAPVRLAAHRLDLDFGGVAT
                     LTRHPGGSYGLTVAGDAGGFALTLGPGKPPLPLRAGDETEAPGALSSSCLPRMTAEGTV
                     SASGREPVRVTGEAWLEHSYGTCPEGAHRPGGAETAWMRVQVQLDNGWDVALLRTSLVE
                     ETTGTEPLPSARQPLDDGDPDPSDPPDVLDALDAPALQERPDRPDRPMATHTTQAQHTV
                     CAVSGPDGHGRQATAELWTTRRWDSSVTMNSYPVATRLRIPEAGGLDVRLVASPYAQEV
                     SSMSLGRAFWHGRCRVEGTMGGRPVGGHARVEFVPPNTISDLEDFMRRIGRITRAEVRR
                     FYPDTADPRVLGTLAGLEGHVPLDEASSRGVHAGLVLPVRHLADLGGKSWRSFASACVM
                     ALFSEDADRYRPLMAVTELLHTGSLIVDDVEDASPMRRGGPTVHEVFGAATAINAGTAA
                     YFVFDRVADDILPDDPALRLRVHRAYLRALRAGHAGQALDITGHEQAMDTAVTTGDASV
                     LLQRLHSTHRLKSGLPVRMFAEVSALLTGAGEAETAAIGAYFEALGVAYQISDDVADLR
                     GIGTVRHGGRRRAAKRVAEDLLNGKVTVPLAHAVPLLPPDRLRAVWEAVRGGRATPQMA
                     EAVAAVLAECGAVDAARAHARALLEEAWPALDRALPPTMPKAILRGLGWYAAEREDDVP
                     DPAADGRPPAPRKPLHLTGDRV"
     gene            12530..13276
                     /locus_tag="AS857_RS14400"
     CDS             12530..13276
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009432 - SOS response [Evidence IEA]"
                     /GO_process="GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608107.1"
                     /locus_tag="AS857_RS14400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SOS response-associated peptidase"
                     /protein_id="WP_058043491.1"
                     /transl_table=11
                     /translation="MCGRYVSTRSPQDLAGLFSVTRWDPEPLLEPSWNVAPTDDVWAVL
                     ERADRESGAIERQLRALRWGLVPSWAKSLATGAKMINARVETVHEKPAYRRAFAKHRCL
                     LPADGFYEWEAVAATPAAKAYKQPYFISPADGQVMALAGLYEFWRDPATGDDGPAQWWT
                     TCSIITTEATDTAGRIHPRMPLAIAPADYDTWLDPAHQDPEELRALLTTPAGGDLGARP
                     VSTAVNDVRNNGPHLLDDAPGADTGA"
     gene            13473..13649
                     /locus_tag="AS857_RS39975"
     CDS             13473..13649
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS39975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_160330231.1"
                     /transl_table=11
                     /translation="MPPPSHYRLPSSEPGTDGDGGMGKHGDGKGGEEGDKQQSPRESDG
                     QWNKPVTDPPKKK"
     gene            13660..14805
                     /locus_tag="AS857_RS14405"
     CDS             13660..14805
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348498.1"
                     /locus_tag="AS857_RS14405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_058043492.1"
                     /sec_met_domain="PCMT (E-value: 6.8e-19, bitscore: 65.5,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTPERLLQILTNKGSLAPGSPWAAAVAQVPRELFLPDGFEAGGR
                     VVDRATHPERWARAVYGDLALVTQVNEGRDLGEDAYQRPTSSSSMPSIMLQMLGLLDVR
                     PGHTVFEAGAGTGYNVAWLCHRLGDRHVTTMDVDPGIAKQAEANLACAGFGPRVLCGDA
                     EDGRPDGAPYDRVLATYTVPEIPYAWVEQAPAGRIVAPWGGGLFHYSFAVLDVADGRAR
                     GRFTGNPAFMHTRSRPAGGGLLRDFLHHEDAGTPGRTTLCPLDVVHDQHALFYVDLAVP
                     QVWHYVGRAQDGSDEATLWLFSYDKGSWATAEYVPGRASYEVEQYGPRCLWDEVEAAYR
                     QWERYGRPGRERAGMTVHPGGQDLWLDEPGNAIAVVPRTGP"
     gene            14944..15708
                     /locus_tag="AS857_RS14410"
     CDS             14944..15708
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379747.1"
                     /locus_tag="AS857_RS14410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminoglycoside phosphotransferase family protein"
                     /protein_id="WP_058044148.1"
                     /sec_met_domain="APH (E-value: 2.9e-09, bitscore: 34.3,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGGNVSDGVVRVGDTVRRPAGPWTPAVHALLNHLHEAGFRAAPR
                     PLGIDDRGREVLTFAPGEVVWPGRFALLEPPRRLARVARLIRDFHDAVEGFTPPADARW
                     QVLVPAEGADIIAHQDLAPWNLVVGGEDEWAFIDWDAAGPGSRLWDVAYAAHGFIPLSA
                     DPAWQRTDAADRLRIFADAYGLDEAERRHLVPLLGRRTRSMHDFLRDQTARGNQPWATL
                     WAEGHGDTWRNDTAYIEEGEEQWLRALLAGEG"
     gene            15675..16278
                     /locus_tag="AS857_RS14415"
                     /pseudo=""
     CDS             15675..16278
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658872.1"
                     /locus_tag="AS857_RS14415"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAACPAGRRGLNHDGTIAREGALDRVPEAFAPVVGAARAHLAGTY
                     GRTRLHGAYLYGSVPRGTAVPGRSDLDLSLPPHVPARPRNQRRPRSRPAALAHAGRRSR
                     HGQRTQGPRPRCRPASRTHRIHPDHAPLGRLDQRPRRVRRTLRPLPPGAGRADAHRRGH
                     RPHALGRPGGARHAHRRPGPLARRPVHSRARGEGSASV"
     gene            complement(16291..17919)
                     /locus_tag="AS857_RS14420"
     CDS             complement(16291..17919)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180229.1"
                     /locus_tag="AS857_RS14420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alkaline phosphatase D family protein"
                     /protein_id="WP_058043493.1"
                     /transl_table=11
                     /translation="MTGHQGHDRTGTASISYLRRRFLTLTAAATVLAFSAEPGRARAEE
                     LGDRVLGDDPFTLGVASGDPLPDAVVLWTRLAPRPWEPDSGLPATGVIPVSWEVAEDER
                     FSRVVRRGRAAADAEFGYSVHAEARGLKAGRTYWYRFRAGSWVSRAGRTRTAPAAGSSP
                     GQLRLGVASCQQYDEGYYTALSHLAREDLDVVFFLGDYIYENAIDARAGDRQLPDGSRL
                     PDVFAHETVTLADYRLRYALGKSDPDLQAAHAAHPWCVTWDDHEVENNYAAHVSQDNDP
                     TDAFLLRRAAAYRAYWENMPLRLPHPTGPDMRLFRRLRYGKLAQFDILDTRQYRSDQAY
                     GDGWKQPGPDSEDPSRTLLGPAQEQWLSNGFRSSSAIWNVLPQQVAFSRRRLSAQNSPL
                     SMDAWDGYPAARKRLLDAARTARITNLMVLSGDSHIHLAMDIKEDFDNPSSPTRGVEIL
                     GTSISSGADGVERPEDWEHLLAVNPHMAYYAKRRGYVVITLTPSQARVDYRTLPYVTRP
                     GAPVSTATSLICRADHPGFERPPSP"
     misc_feature    17951..17968
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.59"
                     /tool="antismash"
     misc_feature    complement(18116..18129)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            18191..18379
                     /locus_tag="AS857_RS14425"
     CDS             18191..18379
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS14425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058043494.1"
                     /transl_table=11
                     /translation="MRRPTAAVSALLAATALVSTAVPAAATPADERGTDRETARGAAAP
                     AGEGKGTALQRALDGAK"
     gene            complement(18435..19406)
                     /locus_tag="AS857_RS14430"
     CDS             complement(18435..19406)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS14430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058043495.1"
                     /transl_table=11
                     /translation="MTEIDYGQFHEWLQQARSAAEGGTTTDPAGTDARWELLRRFQEGW
                     GYEPTGGERWEREEPDAHKAYVSGLKDRANADADADAGTDADTACSDAPVDDVPHDVDP
                     SLAIPAALDEWWELPFNSFADRWRLYWTNPVWPPTVRPDPTGYGVAGGLPPENPFTGPS
                     EDLRVCVFMAEDQYCNEWGYPAARSHLADPPVLVSAQDDDGQEAWVLQSRSLSEFFLLL
                     AVTRLPAHYGWTVEEGSIDPEVPARLRESLRPMGLLPWRELGARTEFFGGPDVIVAHDT
                     GYGDSELVAYGRTEHALERLAETLGVDWSESICEPESHREEE"
     misc_feature    19496..19511
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.63"
                     /tool="antismash"
     misc_feature    complement(19498..19509)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     misc_feature    complement(20885..20901)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.36"
                     /tool="antismash"
ORIGIN
        1 cgccgagacg aagcacgtcc tgagcgccgc ccggggcggc ctgtccctca acggagaggc
       61 accgcagacc gaccggctcg cgcagatcgc ccgcaccaag aaggtcacgc tcgtggccct
      121 ctccatcggc gggaacgacc tcggcttcgg caccgtcatc ggcgactgcg cctacgactg
      181 ggcgttcagc cgcctgtgct ggaagaagca ggccgcggag gtcgaccgta aactgcccgc
      241 cgtccaggcg aaggtgtccg cggtcgtgga cgacatccgg gcgacgatgg cggccgccgg
      301 ttacggcgac ggcgactacc ggctcgtcct gcagtcgtac ccgtcgcccc tccccggcgg
      361 cgaggagttc cgcctgcgcg aaggcgaccc gaaccgcctg cgcagggacg gctgtccctt
      421 caacgaccgg gacgcctcct gggcccgcta caccttcgtc ccccagctgt ccgccatgat
      481 ccgtgacgtc gccgcgtccc gcggcgcgga cttcctcgac ctcaaggaca cgatggcggg
      541 acacgaggtc tgcgccctcg gaaccaccca ggtgggagcc accggccccg acgcccgtaa
      601 gcacgagtgg ttccgtttcc tcgactacgc caacacccag gggacgctgg aggaatccat
      661 gcaccccaac gccttcggcc agcgggcgct gggtgcctgc ctgggcctcg tggcggcggc
      721 ccggcccggc cggtacggat gcgtcgccga ctacctcggc gacggctcgc cgaccgccat
      781 gcgcatccgc ccggcggatc aggccgggcg ctgaacgggc ctttcagacg cgcgtggtgg
      841 cgaacggccc gccggagccg aaggccaggg cggcgaagcg ttcgccgatg cggcggtgtg
      901 ccgcggcatc cgggtggagc tggtcgggca gtggcagttc ggcgaagtcc gcctcgccgt
      961 agagctcgcg gccgtcgagg tagtgcaggt tcgggtcgtc ggccgcccgc tgctccacca
     1021 ggcgggccag ttcgtcccgg atgacgttga gggtcagctt cccggcggcg cgctccgcgg
     1081 ggtcgcccgc ggcccggaac cgcagcttcc cctcgctgag gctgctgaag tccggggcgc
     1141 tggggccggg ggtgtcctcg tggatggggc acaggatggg ggagacgacc aggagcggcg
     1201 tggtgggatg gccctcgcgg acggtgtcga ggaagccgtg caccgcgggg ccgaaggcgc
     1261 gcaggcgcat caggtcggtg ttgacgaggt tgatgccgac cttgatgctg atcacgtccg
     1321 cgggggtgtc gcgcagggcg cgggcggtga acgggtcgag cagcgcgctg ccgcccagcc
     1381 ccagattggt cagttccacg tcgccgagga agccggccag cgcgggccag gtcgtgctgg
     1441 gactcgcggc atcggagccg tgactgatcg aactgccgtg atgcagccac ttcttgcggc
     1501 ccccgtccgg caggggctcg acgggagcgt ccgtgcgcag ggcgacgagc tgggtgatct
     1561 cgttgtgcgg cagccagatc tcgacgtcct tgacgcggtc gggcagaccg gagaaccgga
     1621 cggtaccgac cgggccggga cgggtctccg cggacccggt ggtcatgtcc accagcagga
     1681 cgttgccgcc ggacacgctc gcccgcccgg ccaggcggcc gtcgacgagc aggtcgtaga
     1741 cgccgtcggg ccggggcggg gcgcccttgt agacgttctt ggtgcggagg gcgtccagct
     1801 cgatgacacc ggcccgcgtg cggaacacca gccgtacgcc ggagggctgg gcctccgcca
     1861 cggccagctg cccgtcggcg cactgcgccc gggcccgggc cggcagccgg tgcggcagga
     1921 ccccgtgttc ggtgtactcc aggtccaggg cgccgcgcag gaggtccgcg gtgatgggcg
     1981 tggtgacggg agtcgtcgtc cagtccgtcg ccggggtcga ggcgggggtc tcggggatgt
     2041 cggagatgtc gtcgagccgg tcgttcatgg gtgcattctg cgggccggat gcgtggaacg
     2101 cccaatcatt atccgttcac cgggcctccg ggcgccggcc gccccgtcgc gcgctcgttg
     2161 cgatgtgctg acgcaatttt ccgcccatcg gaggaatctg gggggaacga gagttggaga
     2221 caccggaggg cgtgcaggct gttccgcgtg acggggcggc ggagaacacg gtatgcggtg
     2281 atgctggcca cggctgctgc gctggcgatg accggcgagg cggcggccga cgacggtggc
     2341 gcgagtgtcg cgaccggcaa tacgtcggtg aacgctgtcc tgcaccgcct gccgagcctg
     2401 ctgccgcctc atcccccgtc gcacgtcgcc gtgaacggac tgggcggcaa tgttcaagcg
     2461 gaagtgaccg acaacggcca agggtgcctg gacgtcactg cgcgcgtgct caacaccgtg
     2521 gacgcagagg tgtggctgaa gtgccgcaac gccaggccgc ccgccccggc gccacctgcc
     2581 cccgcccctc cggcccccgc ccctccggcc cccgcccctc ctgcccctgc gccgcccgcg
     2641 cccgctccgc cggccccgcc cgctcccgtc cccccaccag gtgcaccgca agtacccccg
     2701 gcccccgcgc ctcctgcccc ggcgcccgta gtcccgcctc ccgcgccggc gcccccggct
     2761 cccgccccac cggctcaact cccgccggcc cccgccccgg ttgtgaggac gccgccccgc
     2821 cccgccccgc ctaccgcccc gccgaacacg gcgcccgttc cgaaaccgcc caccaacctg
     2881 tgcggaccca ggaaccgttg cccctcctac cggaaggtcc tcccgtcagc gaccgagaag
     2941 cagctccggg gcctgtcgct ggtgaccctc accctgctgc tcaccacccc ggccgtgctg
     3001 gccgcggcgc tgctgcggcc gcgctccagc agccgacgaa accggtgagg ggccggagcg
     3061 tgcgttgcaa ccgtgcccgt cggcggggaa ggttgtggtc tccgatggat cgacggcggt
     3121 ggtgcggatg gcgcgtgtgg tcgtcctggg ggagttccgc gcggacagga tcgtgccacc
     3181 gttcagggac ggcggcgcga aggtcaccgt gctgggattc gcggacctga cgtcgttcct
     3241 gggccccggg acggcgtgcg gagagctgcc gacgactctc gacgaacgca ggacgctgca
     3301 gctgctcgag gagcaccggg ccgaagtcgc cgttcccaac atggggtgtc ccggacagga
     3361 gcagctcctg cccgtctact cccgggcggc atcccggctg cgtggcgcgg gctgccgtat
     3421 gcccgtgcac ccggaggggt tcgcgacgtc ggccagcgac aaggtggcgt tccaccgcct
     3481 cgcgggggag cggggctggc ccgtgccgcg gggcgtcgtc tgcgaggcgg cggcggagct
     3541 gccgggtgcc gtgggcgaac tcgggttccc cgtgctggtc aaggaggccc gcagcgagtt
     3601 ccacgccgga cgccactacg tacgggaccg ggagcatctg gaccgggtgt ccggcgaggt
     3661 gaggtatccg gtactggtcc aggaggcggt gtcgggggag gagtacacgc tggagctcct
     3721 ctccgggccg cggtcctcgg cggccgggcc atcggccacg gtcgcgtggc cggttgcctc
     3781 cctgggccgg ctaggcagcg attgtgctcc gggtcggcgg gtgcgagtcg caccggcggt
     3841 gctgcccgcg cgagtgcagg aggaactggc cgccacggtc gtggacatgg tggagtcgac
     3901 agctcccctg ggcccgtggc agatggactt cgccgtcacc gacgacggcc ggctgcaggt
     3961 gatcgagctg aacggccggc tgggcggcgt gtccaacatg agctgggcga gtacgggact
     4021 cgacccgcac gtggcgtacg cgcacgctgt gctgggccgt gcgccgtcca cgcggcgggc
     4081 ccgcgcccac cgggtggcgc tcgaactgcc cgtgcgcaac ggggccgccc tgccgccccc
     4141 gccgacgggg acggagctga tggccttccc gggaaacccc gccaaccggg gccccttcat
     4201 cggcgggtac caccgtcccg tcctgtgcgt cccgggcgac cgagccgatg tggtgagcgc
     4261 ctggctgcgg tccctgccgc cgggaacgct gctgaacggg gcggacgagg cggctgctca
     4321 gctggggcgc gggctgtacg ccctgcggcg ggacggcggc cggcctccgc tgtgaaagcc
     4381 cgacagtctc cgctgtgacg gcctccccgc cggcatcggc acggcgtcaa cggcctcccc
     4441 gcccgcgtcg gtacggcatc atgcgaggag gttggtgatg agcttgcgca ccctgccctc
     4501 cgtgaccggc aggacgccgt ggaccagctg ggtgccgtag acgacggtgg tgcccggcgg
     4561 cgggctgcag atccagcggg tgtgcggcag gtcgtcgagt ttcaccgggc ccgagcgggc
     4621 ggtgatctcg tggacgagcc gcgttccggg cgcgtagtcg ctttcggccg ccggggtggt
     4681 gtcgctccac agggcgtcgg ccgagtaggt gtcgaggtgg aactcgccac cccgttcggc
     4741 gtcctggagg ttgaaggcga cccgggcgat ccgctgcggc cgggcctgcg ggtcgcccac
     4801 gccgtgaacg tgcggacgga tcttgtcgcc gggtttgagg tcgtggtaca gccacggatc
     4861 ggtgcccgag gcggagggga acacccggcg cagcgcgggc agggcccgcc gcacggcgcc
     4921 gttcaggagg gcctgtgccg cgtcgggcgg ggtcgttccc tcgctgggcc gtacgggaac
     4981 ccagccggtg gccgcgaggt gttcgtccag gaccttggtc agctcggccg tctcgtcggc
     5041 ggtgaggaag tcctcgacgg tctgcacggc cagggtctcg gtcatgtgga tcactggcgg
     5101 actcctcccg cgggcccggt ggcccggccg tctcattcca gtgcgggacg gtgccgaagc
     5161 caagcgccgg cttcggccaa tcccgaaggg ccgcttgacg gtcctcgctc atgcggcgaa
     5221 aggtgaggga gtgacgtccc ggggcgcagg catcgacgca ggcatggacc caggcatgga
     5281 caccgaagtg ctcgtcgtgg gggcggggcc gacgggactg ctgctggcgt gcgaactggc
     5341 actggcgggc gtacgggtcc gggtggtcga caagcgcgcg tcgccccacc gggagtcacg
     5401 ggcgctcaac cttcacccgc gcagcatcga gctgatggac atgcgcggcc tggccggccg
     5461 cttcctggcc gcaggccgca ccgtgcccgg ctggcagttc gccgtcagcc tcaagcggcg
     5521 gctggacttc accgggctcg acacccggca cccctacgcg ctgctgctgg cccaggcacg
     5581 gaccgaggcg ctgctggcgg aacgggcccg ggagctgggg gtggaggtcc accgcgggtg
     5641 cgaggccgtc gcagtcgggc aggacgccca cggcgccgtg gccggtctct ccggacccgg
     5701 cggcccggtc acgattcgct gcgcgtacgt cgtcggttgc gacggcgggc gcagccgggt
     5761 ccggcaggcc gccggcatcg ggttcccggg gacgcgcgag tccatgagcg cggtcgtggg
     5821 ggacttcgcc gtcgccgacc acgcgtgcaa cgaacgggcc cggcagcacg gcgtcctcgt
     5881 cgcccgcctg gagagcgggc tgacccggtt cgtggtcatg gacccccggc ggatccggac
     5941 tccctcctcc gaaccggtga ccctggagga gttccgcacc tcactgaggc aggtgtgcgg
     6001 caccgactgc ggcatcggcg agccccggtg gctgtcgagg ttcggcaaca ccacccgcct
     6061 cgccgggacc taccgccgcg gccgcgtcct gctggccggc gatgccgcgc acgtccactt
     6121 ccccgtcggg gcccagggcc tgaacaccgg gctgcaggac gccatgaacc tcgggtggaa
     6181 gctcgccgcc gcggtccggg gctgggcgcc gcccggtctg ctggacacct atcacgccga
     6241 gcgccacccc gtcggccacg ccgtcaccgc gggcacccgg gcgcagaccc tgctggtgga
     6301 actccccctc accgagcagt acggactccc tgcggagctc ctgtgcgagc tgctcgacac
     6361 gctcctgggc atgagcgagg tcaaccggta cctggccggc cggatctccg ccctcgacac
     6421 cgcctatcct ccgtccctgc cggacgccga cccgctcacc ggccggcgca tgcccgatat
     6481 cgacctgacg acggcggccg ggccggcccg cgtgtacgaa ctcctccgcg acggccgctt
     6541 cgtcctgctg cacttcggcg aaggcacggg cccggacgag tcctcccgca cccgcgcagt
     6601 caccgcccgc cggcacgagg cccggtcgga cctcgacggc gtgagcgccg tactcgtcag
     6661 gcccgacggc cacatcgcct gggcgacccg caccaccgac ccgcgtgccc tgaacgccga
     6721 acgggccgag gcgctgctgg cgtggaccgg cacccgagtg ggctgacggc gaggctctcc
     6781 tcgcggtcga gccggccggt cccgggcccg cacgccccgt cgagggtcac gagaaggaga
     6841 tgttgccccg ccccgcgacc gtccgcagac cgcgggccgc gcagccggtc acgtcggtgg
     6901 tgggcaccgc ccagccgcgt acgtggcggt ccgccatccg ccccgagacg acgtcgccgc
     6961 tgaggaaggc gtccgcaggg gtgtagccgc caccgccgcg cgaggacggc ctcaggtggg
     7021 aggttccggc ggtccggccg cgctgggccc agccgtggta ccaggtgatg acggcggacc
     7081 cctcggggcg ggcggcggtg aggcagttgg ccgtcgccgc gccgcgcagg accatccttc
     7141 cggaccggat cccgtactgg ctgccgtccg cggaggtgca gcggtcgagc aggaccgtac
     7201 ccgtggccga cacccgctgc acgcggccgg tgacgggcgg gcggaagacg agggggtcgc
     7261 cgggggcggt ctgcagggag caggacaggg cggtcccggc caaggccgta ccggccgggg
     7321 ccgccccggt cacggcggtc acggccggag cggtccgggc tgtgccgcgc gcgggggcgg
     7381 ccgacgccgg cgtggtcccg ggaccggcgg tcgtgcagag ggcgccgagc agcagggcgc
     7441 ccagagccgc gtggatcgtc gcggtgcgcg gacggcgcgg acgacgggcg cggcgcggac
     7501 ggtgcgtggt ggtcatgtgc ggatcttctt cctgacggtc tgtttctgct gggccggcac
     7561 ggcacccgtg ggtgccggcg gcacgaaggc ggtgtggagt ccgaggcgcc ggtggagacg
     7621 ccgcagcggc gcgggcgccc accagttggc gcgcccggcc aactgcatga aggcgggcac
     7681 gaggacgccg cgtacgacgg tggcgtccac gagcacggcg agggcgatgc cgcatccgaa
     7741 catcttcagc agcaccacgt gcgaggtcag cagcggcagc acggcgatgg cgacgagcag
     7801 cgacgccgtg gtgatcaccc ggccggtgcg ggcgatgccg ttgacgacgg cggtgcggca
     7861 gtcggcgccg gcccggtact cctcctggat gcgggcgatg aggaagagct cgtagtccac
     7921 cgagaccccg aaggcgaagc agaacatcac caccggcatg gccgtctcca gctcgcccgt
     7981 cacggcgaac gacccgagga ggccgcgcag gtggccgtcc tggaagacgt ggaccatggc
     8041 cccgaagctg gcgcacatgc tcaacgtggc cagcaccacg gccttgagcg ggatgagcag
     8101 cgagccggtg aaaaggaaca gcaggaccag cacgcagccg atcaacagcg ccccgatgaa
     8161 gggcaggacg tgcgccaggg ccgtcttggt gtccagcacg cgcgccgggc agccggtgac
     8221 caggtgtggg ccgggggagg gcagcgcgcg cagggtctgt acgaggtccg ggtcgctcgc
     8281 gggcggaccg gatcccgtca ccgcgagcca ggtgccgccc atggtgatct gcagcagcga
     8341 tccgggcgtc ggcccggcca cctggcgccc gtgggcgtac gtccccaggc ccgtcaccac
     8401 gctcgcgacg tcgggccgtt cggacagctg ccgggcgtag gcggtcacct cggcctcctg
     8461 ctcgacggca tccgtggccg gcaacaggac ggtgacggcg cgatcccacg ggagggagaa
     8521 gtcgcgctcg atcccggcgg cgacggcgtg ggactccagc ccggccggca gcacccggta
     8581 gtcggtgacg ccgaagcgga cgtgggcgaa cggcagcgcc gacgcggcga gcagcagcac
     8641 gcagcagccg ccggccagga cgggccggcc ggtggcggca cgggcgacgc gccgccacag
     8701 cgggctctcc gcggaacggg cccggcgcca ccgggccagc gggtcgccgc ggtcgatcca
     8761 gcggccgagc agaaccagca gcgccggcag gacgacagcc gtggcgagcc cgcagcacag
     8821 cacgaccgcg atgcccgcgc aggccagaga ccgcaggaac ggcaggggga acagcagcat
     8881 gggggtcagg cagaccgcga tcgtgccgct ggagaagaac accgtgcgcc cggcggtgcg
     8941 catggtgcgc agcgtggcgc cccgggtgtc gggtctgccg tcggcatcgt gcgcgtcgcc
     9001 ttccacggcg gcgagttcct ggcggtagcg gctgacgagg agcaacccgt agtcgacggc
     9061 cacgccgaag ccgagcgccg tcgcgacgtt cgtggcgagc gcggacacgg gcatcacgcc
     9121 ggccagcgca cacagcagcg gataggcgcc cagcaccgcg aacagcccgg tcagcaccgg
     9181 cagcagggcc gcgaccgggg aaccgaaggc cagcagcagc gccagcgcgg ccagcggcac
     9241 gccgagcagc tcggcccgga gcagctcctg cccgctgacc tcggtggcct gcgccgagac
     9301 gtaggaggtg ccggtggccg acacccgcag cggaccgtgg gcaccggtga agcgcggcac
     9361 cagccgtgcg gcggcccgcg ccgtggccgt gtcgtccccc gccaggtcgg ccacgacgag
     9421 ggcgctgcgc ccgttctcgc tcagcagctc ggaggcgccc gtgttccagt acgagtagac
     9481 gcgctcgacg cccggctgcc gggagagcct gacggtgagg tccaggcccg cgcgggagac
     9541 gtcctccgaa ccggcggggg agcgggcgtc cgcgcgcagc accaggtccg gcaggcccgc
     9601 gtggaagcgc tcggccagga cggcgtcggc gcgagcggcc tcggtcccgg tggcgagata
     9661 gccgccgccg ctgaactgag ggcgggctcc cagcagccac agccccagca ccgcccccag
     9721 gaggcccgcc ccggccagca cccgccaggg acgggaaacc acccaccgag tcatcatctt
     9781 cgtccaccgt ccccatccgt cgtgtcccgg gtatacggcg gcggccgggc acccctgcgg
     9841 gcggcacgcc cgcgctcacc cgaatgcacc cgacagggca tggatcgccc gccacgggcg
     9901 atccatacgg gtggtcgcag gtaaccgtgt tgacgctcgt gcggttgggc ggttacggcc
     9961 agtacgcaac ccgcaccgca ggactcccct tggggcaccc gtgaacaagc acgccgcaca
    10021 ggcaccgccg tccccgtccg ccaccgactc cggcgagtcc gtacgcgagg acctgcccca
    10081 cccggaccgt cccgtcgaac ggtggtccgt caacgcctcg gtacggacgg ccggaggcga
    10141 agagcacagc ctgtccgcgc agttcctgtg ccggcggccg ggcgccgggg gagggaccgg
    10201 aaccgcccgg cccggcgggg gagcggaccg gcccgccggc cacaccgtca tctggtcgca
    10261 caccgaccac gccaccggcg gccgcaccac cgagtcctgg ctggacgagg cggccctcgc
    10321 cgaggcacgc tccgccttcg aggacgcctc ggcgtgggac ccacacgtgc gccgggccgt
    10381 cctcgacgcc ctggcgggcg gcaggccgct cctgcccgac cggctgctgc acgcacccgt
    10441 acgcctcgcc gcgcaccggc tggacctgga cttcggcggt gtcgccaccc tcacccggca
    10501 cccgggcggt tcgtacggcc tgaccgtggc cggcgacgcc gggggcttcg cactgaccct
    10561 cggcccgggc aaaccacccc tgccgctgcg cgcaggcgac gaaacggaag cgcccggggc
    10621 cctgtcctcg tcctgcctgc cgcgcatgac ggccgaaggc accgtgtccg cctccgggcg
    10681 cgagccggtg cgcgtcacgg gagaggcgtg gctggagcac tcctacggca cctgcccgga
    10741 gggcgcgcac cgccccggcg gcgcggagac cgcctggatg cgggttcagg tgcagctgga
    10801 caacggctgg gacgtggcgc tcctgcgcac cagcctcgtc gaggagacca ccggcaccga
    10861 gccgttgccg tcggcccggc agcccctcga cgacggagac cccgacccgt cggatccgcc
    10921 ggatgtgctg gacgcgctgg acgcgccggc tctccaggaa cggcccgatc ggcccgatcg
    10981 gcccatggcc acccacacga cgcaggcaca gcacacggtg tgcgccgtct ccggccccga
    11041 cggacacggc cggcaggcga cggcggagct gtggaccacg cgccgctggg actcctcggt
    11101 caccatgaac tcctaccccg tcgccacccg gttgcggatc cccgaagcgg gcggcctcga
    11161 cgtacgactc gtcgcgtccc cgtacgcgca agaggtctcg tcgatgtccc tgggccgcgc
    11221 cttctggcac ggccggtgcc gggtggaggg cacgatgggc gggcggcccg tcggcggcca
    11281 cgcgcgcgtg gagttcgttc cgcccaacac catcagcgac ctcgaagact tcatgcgccg
    11341 catcggccgg atcacgcggg cggaagtccg ccggttctac cccgacaccg ccgacccccg
    11401 ggtgctgggc actctggccg ggctggaagg ccatgtcccg ctcgacgagg cctcgtcccg
    11461 cggggtgcac gcagggctcg tcctgcccgt acgccatctc gcggacctgg gcgggaagtc
    11521 gtggcgctcg ttcgcctccg cctgcgtcat ggccctcttc tccgaggatg cggaccgtta
    11581 ccgccccctg atggccgtca ccgagctgct gcacaccggg tccctcatcg tcgacgacgt
    11641 ggaggacgcc tctccgatgc gccgtggcgg ccccaccgtc cacgaggtct tcggcgcagc
    11701 gaccgccatc aacgcgggca cggccgccta tttcgtcttc gaccgggtgg cggacgacat
    11761 cctgcccgac gaccccgcgc tgcgcctgcg cgtgcaccgg gcctacctgc gcgccctgcg
    11821 cgcgggccac gccgggcagg ccctcgacat caccggccac gagcaggcca tggacaccgc
    11881 cgtgaccacc ggcgacgcct cggtgctgct gcagcggctg cactccaccc accggctgaa
    11941 gtccggtctg ccggtacgga tgttcgccga ggtcagcgcc ctgctgaccg gcgcggggga
    12001 ggcggagacc gccgccatcg gcgcgtactt cgaggccctc ggcgtggcct accagatcag
    12061 cgacgacgtc gcggacctgc gcggcatcgg caccgtccgg cacggcggac gccgccgcgc
    12121 ggccaagcgc gtggccgagg acctgctcaa cggcaaggtc acggtgccgc tcgcccacgc
    12181 cgtgcccctg ctgccgcccg accgtctgcg cgccgtgtgg gaggccgtgc gcggcggccg
    12241 cgccactccg cagatggcgg aagccgtcgc cgccgtcctc gccgaatgcg gcgcggtgga
    12301 cgccgcccgc gcccatgccc gggccctcct ggaggaggcc tggccggccc tcgaccgcgc
    12361 cctgccgccg acgatgccca aggcgatcct gcgcggcctg ggctggtacg cggccgaacg
    12421 cgaggacgac gtccccgacc cggccgcgga cggccggccc ccggccccgc gcaagccgct
    12481 gcacctgacc ggcgaccggg tctgacgaga ggaccggcga ggttttgcca tgtgcggccg
    12541 ttacgtctcc acccgcagtc cgcaggacct ggccggactg ttctccgtca cccggtggga
    12601 cccggaacct ctcctggaac cgagctggaa cgtcgccccg accgacgacg tatgggccgt
    12661 gctggagcgc gccgaccgtg agagcggcgc gatcgagcgg caactgcggg cgctgcggtg
    12721 gggcctggtg ccgtcctggg cgaagagcct ggccaccggc gcgaagatga tcaacgccag
    12781 ggtggagacg gtgcacgaga agcccgccta ccgccgcgcc ttcgccaagc accgctgcct
    12841 gctcccggcc gacggcttct acgaatggga agcggtcgcc gcgacccccg ccgcgaaggc
    12901 ctacaagcag ccgtacttca tcagccccgc ggacggtcag gtgatggcgc tggcggggct
    12961 gtacgagttc tggcgcgacc cggcgaccgg agacgacggc ccggcgcagt ggtggaccac
    13021 ctgcagcatc atcaccaccg aagccaccga caccgccggc cgcatccacc cccgcatgcc
    13081 gctggccatc gcgccggccg actacgacac ctggctcgac cccgcccacc aggaccccga
    13141 agagctccgc gcactcctga ccactcccgc gggcggcgac ctcggcgccc gcccggtgtc
    13201 gaccgcggtc aacgacgtcc gcaacaacgg cccccacttg ctcgacgacg cccccggcgc
    13261 ggacacgggc gcctgacccc ggtccgctca accctccgga ccggagcgcc atcctggccg
    13321 cccagggcca caggaaatgc ccgctggcca gaagtggccc atccggccga ccccctgctc
    13381 cgaggccgtc ggcgagcggg gttcggcctc cggccgatac gtatcggcca acgcacggcc
    13441 aaccttcggc cgacgtgcgc aaaggacccg ccgtgccgcc cccctcccac taccgtctgc
    13501 cctcatcgga gcccgggacg gacggggacg gcggcatggg caagcacggt gacggcaagg
    13561 gcggggagga aggcgacaag cagcagtcgc cccgcgagag cgacgggcag tggaacaagc
    13621 ccgtgaccga cccgccgaag aagaagtgat cgcttccaga tgagcactcc cgaacgactg
    13681 ctgcagatcc tcacgaacaa gggctccctc gctcccggct ccccgtgggc cgccgccgtc
    13741 gcgcaggtgc cgcgggagct gttcctcccg gacggtttcg aggccggagg gcgcgtcgtg
    13801 gaccgtgcga cgcaccccga gcgctgggcg cgggcggtct acggggacct cgcgctggtc
    13861 acccaggtca acgagggccg cgacctcggc gaggacgcct accagcggcc gacgtcctcc
    13921 agctccatgc cgtcgatcat gctgcagatg ctcggcctcc tcgacgtccg gccgggtcac
    13981 accgtcttcg aggcgggcgc gggcaccggc tacaacgtgg cctggctctg ccaccggctc
    14041 ggcgaccggc acgtgaccac catggacgtc gacccgggca tcgccaagca ggcggaggcc
    14101 aacctcgcct gcgcgggctt cggcccccgc gtcctgtgcg gcgacgccga ggacggccgg
    14161 ccggacggcg caccctacga ccgggtactc gcgacctaca ccgtgccgga gatcccgtac
    14221 gcctgggtcg agcaggcccc cgcgggccgc atcgtggcac cgtggggcgg cggcctgttc
    14281 cactacagct tcgccgtcct cgacgtcgcc gacggccggg cccgaggccg cttcaccggc
    14341 aacccggcgt tcatgcacac ccgcagccgg cccgccggcg gagggctcct gcgcgacttc
    14401 ctccaccacg aggacgccgg gacgccgggc cgcaccaccc tctgccccct cgacgtggtc
    14461 cacgaccaac acgccctgtt ctacgtggac ctggccgtgc cccaggtgtg gcactacgtg
    14521 ggacgggccc aggacggaag cgacgaggcc acgctctggc tgttctccta cgacaagggc
    14581 tcctgggcca ccgccgaata cgtccccggc cgggcctcct acgaggtcga gcagtacggg
    14641 ccccgctgcc tgtgggacga ggtcgaggcc gcctaccggc agtgggagcg gtacggccgc
    14701 cccggacgcg agcgcgccgg aatgacggtc caccccggcg gacaggacct gtggctggac
    14761 gagccgggca acgccatcgc cgtcgtcccc cggacgggtc cgtagatccg ggcccgtaaa
    14821 tgaggtgatc gcaaggggcc cgtcgagcag acttgcgccg tgcagcacgg cgaagagcag
    14881 catgacgaga aacaccctga tgagcagcgc cctgtcgagg agcaccccga cgaggagcag
    14941 ccgctgtccg gcgggaacgt cagtgacggc gtcgtccgcg tcggcgacac cgtccgccgc
    15001 cccgcgggcc cgtggacccc ggcggtgcac gcactgctga accatctcca cgaagcgggc
    15061 ttccgggcgg ctccccgccc cctcggcatc gacgaccggg gccgggaggt cctgaccttc
    15121 gcgccgggag aggtggtctg gcccggccgg ttcgccctgc tggagccgcc gcggcggctg
    15181 gcccgggtcg cgcgcctgat cagggacttc cacgatgccg tggaggggtt cacgccaccg
    15241 gccgacgccc ggtggcaggt gctcgtcccc gccgagggag ccgacatcat cgcccaccag
    15301 gacctggcgc cgtggaacct cgtggtcggc ggcgaggacg agtgggcgtt catcgactgg
    15361 gacgcggccg gccccggttc gcggctgtgg gacgtcgcct acgccgcgca cggcttcatc
    15421 ccgctgtccg cggaccccgc ctggcagcgc accgatgcgg ccgaccggct gcggatcttc
    15481 gccgacgcct acgggctcga cgaggccgaa cgacgccacc tggtccccct gctgggccgt
    15541 cgcacccgct ccatgcacga cttcctgcgc gaccagacgg cccggggcaa ccagccctgg
    15601 gccaccctgt gggccgaagg ccacggcgac acctggcgca atgacaccgc atacatcgag
    15661 gagggcgagg aacagtggct gcgtgccctg ctggccggcg agggctgaac cacgacggga
    15721 cgatcgcccg cgaaggcgcc ctggaccggg tgccggaagc gttcgccccc gtcgtcgggg
    15781 ctgcgcgcgc ccacctcgcc gggacgtacg gccgcacgcg gctgcacggc gcctacctct
    15841 acggcagcgt cccccgcggc accgccgtcc ccggacgctc cgacctggac ctgtcgctac
    15901 cgccccacgt ccctgctcgc ccgcgaaacc aacggcgacc tcgctctcgc cctgccgcgc
    15961 tggcgcacgc gggccgccga agccgccacg gacagcgaac gcaaggccct cgcccgcggt
    16021 gtcgcccggc gtctcgtacg caccggattc accctgatca tgccccactg gggcggctgg
    16081 accagcgacc tcgacgagtc cgccgaactc ttcggccgct accacccgga gcgggccggg
    16141 cagatgcgca ccgccgcggc caccggccgc acgccctcgg ccgacccggc ggcgctcggc
    16201 atgcccaccg acgacctggg cccctggctc gccgcccggt acacagccgt gcacggggag
    16261 aaggctccgc gtccgtgaac ggcatcgccg tcacggagac ggcggtcgtt cgaatccggg
    16321 gtggtccgcc cggcagatca ggctggtggc ggtggagacg ggagcgcccg ggcgggtgac
    16381 gtaggggagg gtgcggtagt cgacgcgggc ctgcgacgga gtgagcgtga tgacgacgta
    16441 gccgcggcgc ttggcgtagt acgccatgtg cgggttgacg gcgaggaggt gctcccagtc
    16501 ctcgggtctc tcgactccgt cggccccgct ggagatggac gtgccgagga tctccacgcc
    16561 gcgcgtgggg gaggaggggt tgtcgaagtc ctccttgatg tccatggcga ggtggatgtg
    16621 ggagtcaccg gagagcacca tgaggttggt gatccgcgcg gtgcgcgcgg cgtcgaggag
    16681 ccgcttgcgg gccgccgggt acccgtccca ggcgtccatg gacagggggg agttctgggc
    16741 gctcagccgg cgccgggaga aggcgacctg ctgggggagg acgttccaga tggccgacga
    16801 gctgcggaat ccgttggaga gccactgctc ctgcgcgggg ccgaggaggg tgcgggaggg
    16861 gtcttcggaa tcggggccgg gctgcttcca gccgtccccg taggcctggt cggagcggta
    16921 ctgccgcgtg tccaggatgt cgaactgcgc gagcttgccg tagcggaggc ggcggaagag
    16981 ccgcatgtcc ggccccgtgg ggtgcgggag gcgcaggggc atgttctccc agtacgcgcg
    17041 gtaggccgcg gcccggcgca gcaggaacgc atcggtcggg tcgttgtcct gcgacacgtg
    17101 tgccgcgtag ttgttctcca cctcgtggtc gtcccaggtg acgcaccagg gatgggcggc
    17161 gtgcgcggcc tgcagatcgg ggtcggactt gcccagggcg tagcgcaggc ggtagtccgc
    17221 cagcgtgacg gtctcgtgcg cgaagacgtc gggcagccgg ctgccgtccg ggagctgccg
    17281 gtccccggcc cgtgcgtcga tggcgttctc gtagatgtag tcgccgagga agaagacgac
    17341 gtcgaggtcc tcgcgggcca ggtgcgacag cgcggtgtag tagccctcgt cgtactgctg
    17401 gcacgaggcc acgcccaggc gcagctgccc gggagagctt ccggcagcgg gagcggtacg
    17461 ggtgcggccg gcgcggctca cccaggagcc ggcccggaag cggtaccagt aggtgcgccc
    17521 tgccttgagc ccccgggcct cggcgtgcac gctgtacccg aactcggcgt cggcggcggc
    17581 ccggccccgg cgcacgacgc gcgagaagcg ttcgtcctcc gcgacttccc agctcacggg
    17641 gatcaccccc gtggcgggca gcccgctgtc cggctcccac gggcgcggcg ccagccgcgt
    17701 ccacagcacc acggcgtccg gcaaggggtc gcccgaggcg acgccgaggg tgaagggatc
    17761 gtcgccgagg acacggtcgc cgagctcctc ggctcttgcg cggccgggct cggccgagaa
    17821 cgccagcacg gtcgcggcgg ccgtcagcgt gaggaatctt cgtctgaggt acgagatcga
    17881 tgcggtgccg gtccggtcat gtccctgatg ccccgtcatg acgtgacgtc tccctccagg
    17941 atcgccggtc actcctgcag aggggatacc caggagacac ccaggaggat gcctcgtctc
    18001 ccatgcaaga acggcgtgca ggcgtatccg ccgccacaac cccgaggccg agccggaacg
    18061 gaccccgacc cggtgtcggg ggaacctgta gggggagagg gtcccgtgat cattcttgat
    18121 cacgatcacc tggaggggcg ggcgtccata cggtgagggg gcatcaccgc tcgacggaag
    18181 gaatccgacc atgcgccgcc cgacagccgc agtctccgcc ctgctcgccg caaccgccct
    18241 cgtctccacg gccgttccgg ccgccgccac acccgccgat gagcgcggga cggaccggga
    18301 gaccgcacgc ggagccgcgg caccggccgg cgaggggaag ggcaccgcgc tgcagcgggc
    18361 tctggacggc gcgaaatgag tgctgcggaa cggtcttggg gaaccggccg gggtcgtccg
    18421 gggtgccggg accgtcactc ttcctcgcgg tggctctccg gctcgcagat cgattccgac
    18481 cagtccacgc cgagcgtctc ggccaggcgt tccagcgcgt gctcggtgcg tccgtacgcg
    18541 acgagctcgg agtcgccgta cccggtgtcg tgggccacga tgacgtccgg gccgccgaag
    18601 aactcggtac gggcccccag ctcacgccac ggcagcagcc ccatcgggcg caggctctcc
    18661 cgcagccggg ccggaacttc cgggtcgatg ctgccctcct cgaccgtcca gccgtagtgg
    18721 gcgggcaagc gcgtgacggc cagcaggagg aagaactccg agagcgagcg gctctgcagc
    18781 acccaggcct cttgcccgtc gtcgtcctgg gcggagacca gcaccggcgg atcggcgagg
    18841 tggctgcggg ccgcgggata gccccactcg ttgcagtact ggtcctcggc catgaacacg
    18901 cagacccgca gatcctcgga cgggccggtg aaggggttct ccggtggcag gccgccggcg
    18961 acgccgtagc cggtggggtc ggggcgaacg gtgggcggcc acaccgggtt ggtccagtac
    19021 aggcgccacc ggtccgcgaa ggagttgaac ggcaactccc accactcgtc cagcgcggcg
    19081 ggtatcgcga gcgaggggtc gacgtcgtgg ggcacgtcgt ccaccggggc gtcggagcag
    19141 gcggtatcgg catcggtgcc ggcatcggca tcggcatcgg cattggccct gtccttcagc
    19201 ccggacacgt acgccttgtg ggcatcgggt tcctcccgct cccaccgctc gccaccagtg
    19261 ggctcgtaac cccagccctc ctggaacctg cgcagcagct cccaccgcgc atcagtgccc
    19321 gcagggtccg tagtagtgcc gccctcggcc gcactgcgcg cctgctgcag ccactcgtgg
    19381 aattgcccgt agtcgatctc tgtcacgcgg gcgatcctaa agcgcaggaa tcgaggccct
    19441 gggccgcagg ttcccgtacc gttgccgcgg cccgccagcg cgttcgtgct cgtgttcgcg
    19501 ttcgtgttcc acagggtcag tcccggcgcc gggcgtccgc ggagcggcgg aggtgttcct
    19561 ctgcccggtc gcgcagggcg ggcgctccgc aggacaccga caggtcccgc gcgcggacga
    19621 agcgtgtcac ggcctcggcg tgacggccgc gggccgcgag gacggatccg gccgtcaggg
    19681 aggcggtggc ctcgtacagc ggcatacggg cggtccggaa ggccagcgcc gcttcgtcgg
    19741 cctgctcggc cgcctccgcc tcctccctgg agagcccgac ctcggctcgg gcgagcaggg
    19801 cgaacccgcg ctgtcccaca gggctgtcgg gcgtcagcgc cgcggcagcg tccccggccc
    19861 agcgcccggc cgccgcgacg tcgcctcgtg ccagctccgc gcgcgtgagc acacggaacc
    19921 accggggacg cgtgagcgac ccgaagcgga ggagttccgt accgccgccc gccgtgagca
    19981 cggcacgcac gcatcccgcc gggtcaccgc tttccagcag gcacggcccg agcattccgc
    20041 cgatcgccgc ccgcggcggt ccgggcgcga ggctgtcccg ggacatcgcc tcctcgcagg
    20101 cggcgagcgc cctcgtgacg tcaccgcgcc acagtgcgac ggcgctgcag gccgatgccg
    20161 ccagcgagcg gggttcgtcg ttgccgatga gcacggccgc ctcggccgcg tcctcggcgt
    20221 gggcctcggc ctcgtccagg cggcccagca gcgtgaggac gtgggcggag gcggcgaaca
    20281 ggtcggcggc cacggcggtc cggccggtga ggcgggccag ccgcagtccg cggtcgaggt
    20341 ggcgcagggc ggtgtcgagg ttcccgcggg tcagctccgt ccacccggcc agggccagga
    20401 cgtcgacgtg cggcgcgagg acccggtcgt cggtgtcgtc gagcgtctgc accgcccgct
    20461 ccaggtgcag ctcggcctgc gccgcgtccc cggccgtggc gtgggccagg gccagcaggc
    20521 tccgggctct ggccgcccgc agcgactcgc gccgtggccc gtcgccgccc ggctccagca
    20581 gggtgcgcag cgcttcttcg ctccattcgg ccgccgagtc cgcatcgccc cggcgcaggg
    20641 ccacggaggc gacaccgagc cgcaggtccg cggtctcggc gggtgacagg ccgtcggcac
    20701 cgtccttcaa ctcgcgcagc agcagggcgg tcgcccggcg gtagtgaccc agctggtact
    20761 ccacgtcggc ccgggtcacg gcgatccggg cccgcaggcg gaggtcgcct gcagggaggg
    20821 cgtgggcggc ggtacggagg atgtcctggc cctcgtgcag ccggcctgcc gtgatcaggg
    20881 ccgtggcctg cttcagcagc agttccggct gcggccgctc ctgccgcggg tcggagagcg
    20941 cgatggcggc gcccagccac tcggcggccg tcacgggtgc gagggccgtc atctggtccg
    21001 cggccgcgac cagggcctcg acggcgaggg cgtctcccgg ccgggccgac tgcctgatgt
    21061 gcggcgcccg ggccgccggc ggcgcatcac gcagggcgag ggcgttcgcc gctctcctgt
    21121 gggcctcgat ccgccagccg gatccggcgc tgtggtagac cgcggtacgc accagcgggt
    21181 ggcggaacgc aaaccgcccc aggagacctc cccggcgcag cagatcgcgc tcggtgagct
    21241 cgtcgaaggc cgccagggcg gctgcttcat ccgctccggc gacgaccgac gccaggtcgg
    21301 cgtcgaagga gtccccgagg actgcgcctg cccgggcaca cgtctgcgtg agtacgggca
    21361 acccgtccag ctcctcctgc agggcggcgc gcaggaactg tggcaggtcc tcgccgcaca
    21421 gcagctcccc ggcgggcgcg gacgcgggcc ggagaacggg tccgggaacc ggccggggaa
    21481 cgggtccggc aaccggccgg ggaacgggtc ggagaacgcg tcggggagta ccccaccggg
    21541 tgagggcctg caggtagaag gggttgccat ggccggcctc gtggagctcc cggatccgtc
    21601 ggcggggcac cccctccccg cacaactccc tgcacatgtc cgcgacttcg gccggggaca
    21661 gcggaccggg ctcgatacgg gtgagccggc cctgcgcctc ggccacggac agtgccgcgg
    21721 cgagccggcg ggggtgggga cgcggccggt agccgagcac cagcaggaag cgcccccggg
    21781 gcgcatgacg tatcagatgc ccgatcagtt cgagcgagtc ctcgtccgcc cagtgcagat
    21841 cgtccagggc gagcatcagc cccgagccgg cgtcggagcc tagtgcttcc agcagggccc
    21901 gcattgccgg ctgcagtccg cagcgcccgg cgaccatcgg ctccgtaccg tccgggtgga
    21961 gccaggccag cgccgggaag acctcgccga gcagggccag tacgtccgtg ggcagctgtg
    22021 cggcccggac ccagtccgtg ttcggtgtga ccagccggtc gtcgagcgcg tccgcgaaca
    22081 ccccgtaggg ggtccgggtg aactcgacgg cccgtccggt caggaccacg aagccctggc
    22141 ggcgggcccg ttcaccgagc tcaccggcca gccgcgtctt gccgatgccc ggctcgccga
    22201 cgatctccac gacggcgctc gcgtccgccc gggcggacgc gagcgcccga tcgagggcgg
    22261 ccagctccgc cacccgtccg acgatccggt tctcctgcgg ccggcccgcg aggtcggcgg
    22321 ctcctgtttc cggcggggcg ggcgtggtgg gcgtgcccgg agtctccagt gcctctgtcg
    22381 cctctgtcgc ttccgccgcc tccggcgtcg ctgacgtcac ctcggtccgc ggcgggcgct
    22441 ccgccgtcgc ggacgtcgcc ggagaggccg gtgcctgccg acgtggggcg cgtggccggg
    22501 atgcg
//
