LOCUS       NZ_LNBE01000003        11206 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_3, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000003
VERSION     NZ_LNBE01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2630732)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 2630732)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2630732)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1861263
            Orig. end                         :: 1872469
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11206
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="16"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11206
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11206
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6206](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6206)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(493..795)
                     /locus_tag="AS857_RS13800"
     CDS             complement(493..795)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571293.1"
                     /locus_tag="AS857_RS13800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thiamine-binding protein"
                     /protein_id="WP_058043393.1"
                     /transl_table=11
                     /translation="MLIAFTLTPVGVGESFPDEIADAVKIIRESGLPYRSDSMYTMIEG
                     EWDEVMDVVRRATEAVAARAPRVQLVLKADLWPGKPGMLDGKISMFEQHMADRGQ"
     gene            complement(832..1980)
                     /locus_tag="AS857_RS13805"
     CDS             complement(832..1980)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093225.1"
                     /locus_tag="AS857_RS13805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MAB_1171c family putative transporter"
                     /protein_id="WP_079110305.1"
                     /transl_table=11
                     /translation="MLTWIENAGIAALWSAALVRAPQAVRHREQRPLWFAVVMIALAMS
                     MHLEPVTAALARIVPGAHWVDLTTHLFSIVDAAAVLWFIHGAAGRHRRTHLLFGTAVAV
                     MAALVLLDVLGPAHARNQIAPSRSTASVPDAYWWVFFAFHLTADTFCGFVCWSYGRHGT
                     PRLLRYGLRLFGTGILLASVLWSLKLVYLCTRSPVFAPLFSPVTGVEAAFMALGVALPV
                     FARIRTHLQYRRSYRGLEQLWQDLTTHTPDVVLRPANRIGAAAVPLQLRLYRRVIEIRD
                     AMIVLRNYIPPTTLESIRQSVGRQSLPEHLVEAHITASWLTAALRNKHDGQKPHAQTAN
                     LTGPTDRDADESARSLTHEIDRLLLVASAYHTTTTAPRPAGK"
     gene            complement(1965..2498)
                     /locus_tag="AS857_RS13810"
     CDS             complement(1965..2498)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS13810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="ParH-like protein"
                     /protein_id="WP_216823967.1"
                     /transl_table=11
                     /translation="MHWRHLRRRCQKVIGRIPVPDPFSAQDLCERLAAERDRPLRLLAL
                     PTPTVPGTPSGMLLSVETQDFILYDGQTSPLHQEHIIVHEIGHLVCNHRSALDDTRLHR
                     HLDITDPQSVRRVLPRIRYGDEQEQEAEMIATLILEAAGRVPAPTLLSGMLGGLESAMG
                     LRVGRPAGASCSPG"
     gene            complement(2777..4003)
                     /locus_tag="AS857_RS13815"
     CDS             complement(2777..4003)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613058.1"
                     /locus_tag="AS857_RS13815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FUSC family protein"
                     /protein_id="WP_058043395.1"
                     /transl_table=11
                     /translation="MPGLRDPLTMLVRRGREPAVVQTVRSTAAAVIAYVVALWLSNEPA
                     PLTAPLTALLVVQVTLYATLTTGVRRVNSVVAGVLIAIGFSVLVGLSWWSLGLIILASL
                     VIGRFVKAGEFVPEVAISAMLVLGVTRVAETAWDRVLETLIGAVVGLLFNVLLVPPVWV
                     QPAGEAIEDLARRMRLLLQDIGEEITGHTPVEEAAARLHAARRLDHDIVQVDASLRQAE
                     DSLRLNPRVKEGLLYRVVLRTGLDALEITAVVLRVTCRTLTDLATHRREESLFPEETAA
                     ALREVFTNMALAVEHFAVVITTHVTTSAEHAEDNLTRELDAARRTRDRAADLLLRQVQL
                     HPRQWQLHGALLAEIDRILDELDVEKRTERLTQELDRHSREQRERYPRLHRIAQRLRKS
                     RYGRRSTLK"
     misc_feature    4069..4083
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.66"
                     /tool="antismash"
     gene            4111..4884
                     /locus_tag="AS857_RS13820"
     CDS             4111..4884
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (11-178). E-value: 2.8e-15.
                     Score: 48.6. Matches aSDomain:
                     nrpspksdomains_AS857_RS13820_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999105.1"
                     /locus_tag="AS857_RS13820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_058043396.1"
                     /sec_met_domain="adh_short (E-value: 2.5e-35, bitscore:
                     119.2, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.8e-34, bitscore:
                     116.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEHQGFLAGKCVLVTGASSGIGAAAAKVFAREGAAVVLTARREE
                     RLAALCEEIRGGGGDAAFVVGDIRDEDDARRAVEFAVERHGRLDAAFNNAGIGWDQEPM
                     HLMKDDAYDAIMDTNVRGVWNVMRHELAAMVEAGSGGSVVNNSSVAGMLAIPAAAPYIA
                     SKHAVIGLTKAAAAEYAPHGIRVNTVAPGTTRSEIIAGWFERNPHIEPELHRLTPQART
                     AEPAEIAEAAAWLCSDRASFVTGAVLPVDGGFTMV"
     aSDomain        4144..4644
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS13820_PKS_KR.1"
                     /evalue="2.80E-15"
                     /label="AS857_RS13820_PKS_KR.1"
                     /locus_tag="AS857_RS13820"
                     /protein_end="178"
                     /protein_start="11"
                     /score="48.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="CVLVTGASSGIGAAAAKVFAREGAAVVLTARREERLAALCEEIRG
                     GGGDAAFVVGDIRDEDDARRAVEFAVERHGRLDAAFNNAGIGWDQEPMHLMKDDAYDAI
                     MDTNVRGVWNVMRHELAAMVEAGSGGSVVNNSSVAGMLAIPAAAPYIASKHAVIGLTKA
                     AAAE"
     CDS_motif       4147..4239
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS13820_0001"
                     /evalue="8.40E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="AS857_RS13820"
                     /protein_end="43"
                     /protein_start="12"
                     /score="15.2"
                     /tool="antismash"
                     /translation="VLVTGASSGIGAAAAKVFAREGAAVVLTARR"
     gene            complement(5001..6206)
                     /locus_tag="AS857_RS13825"
     CDS             complement(5001..6206)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="AS857_RS13825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_058043397.1"
                     /sec_met_domain="AfsA (E-value: 6.1e-16, bitscore: 55.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQILLSPAPRADQSTQQHADRSVPCRNVRLQADRIDASGRLRAS
                     AADLSAEDSRAGNGAGDTADGSGAGGSGRVAKELVHLSFDDAVYLTGCEQQDATHYTVS
                     AVWPAHTAHRIPGHGTHYETLTVAQTIRQAGLFLAHTAYAAPLDHATLLRTFSYTLNPD
                     AAVDTHRATPLHVNITATPTTRNKRTTALHLHMTVTAAGSGDAGDEASETVLATGETDF
                     EWIPPMVYRRLRGAYHRPPTTLPPVTPPLPPVQVGRTTPDEVVLSPTAQPRTWQLRHTF
                     DNTVLYDHAVDHVPGLVLIEAANQGAYTVTAPVTEAVTAGTDAATDVDPCACAPDAAGT
                     GYRPQPTATHTTFHRYAEFDAPITLTTETRTTSHTVTHTTVGLQNDQPVFTTTHTAPAP
                     VA"
     gene            6513..7178
                     /locus_tag="AS857_RS13830"
     CDS             6513..7178
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886440.1"
                     /locus_tag="AS857_RS13830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_058043398.1"
                     /transl_table=11
                     /translation="MAKQARAIHTRRLILEAAGEVFDEYGYESATIAEILGRAGVTKGA
                     LYFHFASKEELARGVLSEALTTEGVMPQESRLQEWVDVGMVLAHRLPREPLLSAAIRLS
                     ADRKARDLFGTSWPGWISFIAGQLAAAKEQGELLPHVDTDRIAVLFVSAWTGVEVLSTA
                     LDGGDSLEERVALLYDTILPGIAVPGALRALDTAPDRGTRVWDAHRAQREQEAASAAS"
     gene            complement(7689..8366)
                     /locus_tag="AS857_RS13835"
     CDS             complement(7689..8366)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985133.1"
                     /locus_tag="AS857_RS13835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_079110306.1"
                     /transl_table=11
                     /translation="MTKQERAMNTRHALIRSAAQAFDQFGYTRAKLAGISAGAGVSSGA
                     LHFHFDTKAAMAAAVESEAARTLHALARHAHRKSPSPLQALVDSSHVIAQQLRWDVVVR
                     AGFHLNCRTAHHSETNLRQEWLECVRRTLERSATHGQTPPQAVREELTAAVVGATAGFE
                     ALARHDRSWLSRQTLTGLWHTLLPQLATPKALTQLQPAGTESVINATGTTADRPAGAEA
                     LRG"
     misc_feature    complement(8380..8392)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    complement(8425..8443)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.3"
                     /tool="antismash"
     misc_feature    8705..8722
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.93"
                     /tool="antismash"
     misc_feature    complement(8719..8732)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.59"
                     /tool="antismash"
     gene            8768..9556
                     /locus_tag="AS857_RS13840"
     CDS             8768..9556
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140399.1"
                     /locus_tag="AS857_RS13840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_058043399.1"
                     /transl_table=11
                     /translation="MDQQVSTLVDAHAGEASRRRVPGPRAGHPADRAPALTRPGETLRI
                     LVVDSDTQSAETLSRRLQRHGCETFSVATGEAALAEYRSADLVLLDPELPDLDGLEVCR
                     ALRAASEVPIIAVTALAGELDRVLGLQAGADDYVVEPYGFRELMARIEAVMRRARPAAR
                     TSAGVITSGALTIDPDLREVRLGGRRIDVTRKEFDLLHLLASRPEKVVSREQLMTQVWD
                     DYWSRPGRTIDTHVSSLRGKLGSAGWIITIRGVGFRFGRA"
     misc_feature    8783..8797
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     misc_feature    9702..9713
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.68"
                     /tool="antismash"
     misc_feature    10128..10141
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 cgaactccgc gatctgcttg tcggagaggt tctgcgcgag gttggcctcc gagatcatgc
       61 cgacgaggcg gtggccgttc tgcacgtcga tcaccggcag ccgcttgatc tggtgctgct
      121 ccatggatgc cagggcgtcc gtcgcgctcg cgtccgcgtc gatccagtgc agctcgccgc
      181 cgagcgaacc cgcctccaca ctcgccggat ccacaccgtc ggccacgcac tggacgacga
      241 tgtcccggtc ggtgaccagg ccctggagcc tgtcgtcgtc accgcatatg ggcagcgcgc
      301 ccacctgcag ctcgcgcatc atcttcgccg cgtcctgcag cgattcgtgc tcgccgacgc
      361 acttcgcgcc cgccgtcatg atgtcgcggg ccttgagcgg cttgttgcgt gccatgatcg
      421 ctcctcccgt cgtggccggt tttcctttct ccatcgcaac acggatgacc acccgacgcc
      481 atgcgcgcac gctcactgcc cgcggtcggc catgtgctgt tcgaacatgc tgatctttcc
      541 gtccagcatg ccgggcttcc cgggccacag atcggccttc aggaccagct ggactcgcgg
      601 cgccctcgcg gcgacggcct ccgtggcccg ccggacgacg tccatgacct cgtcccactc
      661 gccctcgatc atggtgtaca tggaatccga gcggtacggc agcccggatt cgcggatgat
      721 tttcacggcg tcggcgatct cgtcgggaaa gctctccccg acgccgacgg gggtcagggt
      781 gaaggcgatg agcacggtgg tccgccgtcc tttctctgtt gtctcttcgt ctcatttccc
      841 cgcgggccgc ggggcggtcg tggtcgtgtg ataggcggac gcgacgagca ggagacggtc
      901 gatctcgtgg gtcaggctgc gcgccgactc gtccgcgtcc cggtccgtgg gccccgtgag
      961 gttcgccgtc tgcgcatggg gcttctggcc gtcgtgcttg ttccgcaggg cggccgtgag
     1021 ccaggacgcc gtgatgtgcg cttccaccag gtgttccggc agggactgcc gccccacgct
     1081 ctgacggatg gattcgagcg tggtgggcgg aatgtaattg cgcaggacga tcatcgcgtc
     1141 acggatctcg atgacccggc ggtagagccg cagctggagg gggacggccg ccgcgccgat
     1201 gcggttggcc gggcgcagga cgacgtccgg cgtgtgcgtg gtcaggtcct gccacagctg
     1261 ttccaggccg cggtaggacc tgcggtactg caggtgggtc cggatccggg cgaacaccgg
     1321 aagcgccact ccgagcgcca tgaacgccgc ctcgaccccg gtcacgggcg agaacagcgg
     1381 cgcgaacacc ggtgaacgcg tgcagaggta gacgagtttg agcgaccaca gcacggaggc
     1441 cagcaggatg ccggtgccga acaggcgcag cccgtagcgc aggagtcgcg gcgtgccgtg
     1501 ccggccgtaa ctccagcaca cgaatccgca gaaggtgtcg gccgtgaggt ggaaggcgaa
     1561 gaacacccac cagtaggcgt ccggcacgct cgccgtgctc cgggacggcg cgatctggtt
     1621 gcgcgcgtgt gccggtccca gcacgtccag cagtacgagg gcggccatga cggcgaccgc
     1681 cgtgccgaac agcaggtgcg tgcggcgatg gcggccggcc gccccgtgga tgaaccacag
     1741 gaccgccgcc gcatcgacga tgctgaacag atgggtggtc aggtccaccc agtgcgcccc
     1801 ggggacgatg cgcgcgagcg cggccgtcac gggttcgagg tgcatggaca tcgccagggc
     1861 gatcatgacg acggcgaacc acagaggccg ctgctcccgg tgccggacgg cctgcggagc
     1921 cctgacgagg gccgccgacc acagggcggc gatgcccgcg ttctctatcc aggtgagcac
     1981 gaagcccccg caggacggcc cacccgcagg cccatcgccg attccaggcc gcccagcatc
     2041 cccgacagca gcgtcggcgc cggcacccgc ccggccgcct ccaggatcag ggtggcgatc
     2101 atctccgcct cctgctcctg ctcgtcgccg tagcggatcc gcggcaggac ccggcgcacc
     2161 gactggggat cggtgatgtc gaggtggcgg tggagccggg tgtcgtcgag cgcggagcgg
     2221 tggttgcaca ccaggtgccc gatctcgtgg acgatgatgt gctcctggtg caacgggctg
     2281 gtctgcccgt cgtagaggat gaagtcctgg gtctcgacgg acagcagcat tcccgacggc
     2341 gtcccgggga cggtcggtgt gggcagagcc agtagacgca gcggccggtc gcgttcggcc
     2401 gcaagacgtt cgcacaggtc ctgtgcggag aaagggtccg gcacaggaat ccggccgatc
     2461 accttctggc atcggcggcg cagatgcctc cagtgcatct cagtcgcttc tcccgcggtc
     2521 gtagggacgg gcgggcacat gacccggtag ccgcgccggg tcatcgtggc cccccggagc
     2581 cggtggtggt gggcgggcag ccccgcacgg catgcttcaa ggcggtcaat ggcagcacca
     2641 cgagcccccc tcgcacatat gcccaagccg cctgcgatcg tagatcgccg cgccgcgtca
     2701 cgccagggtt gcggaacgtt gccggggaaa ccggggaaac cggggaatgc cgcccgcgaa
     2761 aaggaaggcc cgggggctac ttgagcgtgc tgcgacggcc gtagcgactc ttgcggagcc
     2821 gctgggcgat gcggtggaga cgcgggtagc gctcgcgctg ttcccgggag tggcggtcga
     2881 gttcctgcgt cagccgctcg gtgcgcttct ccacgtcgag ctcgtcgagg atgcggtcga
     2941 tctccgccag cagcgctccg tgcagctgcc actgccgggg atgcagctgc acctggcgga
     3001 ggaggaggtc cgcggcgcgg tcacgggtcc tgcgggcggc gtcgagttcg cgcgtgaggt
     3061 tgtcctcggc gtgttcggcg ctggtggtga cgtgcgtcgt gatgacgacg gcgaagtgct
     3121 cgaccgccag ggccatgttc gtgaagacct cccggagcgc ggcggcggtc tcttcgggga
     3181 agagcgactc ctccctgcgg tgggtcgcga ggtcggtgag ggtacggcag gtcacgcgca
     3241 gcaccaccgc ggtgatctcg agcgcgtcca gcccggtgcg cagcacgacc cggtagagga
     3301 gcccctcctt gacgcgcgga ttgaggcgga gactgtcctc ggcctgccgc agcgaggcgt
     3361 ccacctggac gatgtcgtgg tcgaggcggc gggccgcgtg cagccgggcc gcggcctcct
     3421 ccacgggggt gtggccggtg atctcctcac cgatgtcctg caggaggagg cgcatccggc
     3481 gggcgaggtc ctcgatggcc tccccggccg gctgcaccca caccgggggg acgagcagga
     3541 cgttgaacag cagcccgacc accgcgccga tcaaggtctc cagcacgcgg tcccacgccg
     3601 tttccgcgac ccgcgtcacc ccgaggacga gcatcgcgct gatcgcgacc tcggggacga
     3661 actcgcccgc cttcacgaac cgcccgatga cgagggaggc caggatgatc agcccgaggc
     3721 tccaccacga cagcccgacc agcacgctga agccgatcgc gatcaggacg ccggccacca
     3781 cggaattgac ccggcggacg cctgtggtga gcgtggcgta cagggtgacc tggacgacca
     3841 gcagtgcggt gagcggcgcg gtcagcgggg cgggctcgtt gctcagccac agcgccacga
     3901 cataggcgat caccgcggct gcggtggacc ggaccgtctg cacgaccgcg ggttccctgc
     3961 cgcgcctcac cagcatcgtc agggggtcac gaagcccggg cacatcgggg ttccttcccc
     4021 ttcccgcccg cctgcgagcc atccgatgcg ccactcgggt ggcgggaggg gccgaccggg
     4081 cctgtgcagc aggatggcag gattcccgcc atgactgaac atcagggttt tcttgccggt
     4141 aagtgcgttc tggtgacggg ggcgagcagc ggcatcggcg ctgccgcagc gaaggtgttc
     4201 gcgcgcgagg gcgccgcggt ggtgctgacg gcacgccggg aggagcgcct ggcggcgctg
     4261 tgcgaggaga tccgcggggg cgggggtgac gccgcgttcg tcgtgggcga catacgcgac
     4321 gaggacgacg cgcggcgggc ggtggagttc gcggtggagc ggcacgggcg cctcgacgcc
     4381 gcgttcaaca acgcgggaat cgggtgggac caggagccga tgcacctgat gaaggacgac
     4441 gcctatgacg cgatcatgga tacgaacgtc cggggcgtct ggaacgtgat gcggcacgaa
     4501 ctcgcggcca tggtggaggc cggatcgggc ggatccgtcg tcaacaacag cagcgtcgcc
     4561 ggaatgctgg cgatacccgc cgccgcaccg tacatcgcgt cgaagcacgc ggtgatcggc
     4621 ctgacgaagg cggcggcggc cgagtacgcg ccgcacggca tccgggtgaa cacggtcgcg
     4681 ccggggacga cgcggagcga gatcatcgcc ggctggttcg agcgcaaccc gcacatcgag
     4741 cccgagctcc accgcttgac gccgcaggcg cgcacggccg agccggccga gatcgcggag
     4801 gccgcggcct ggctgtgcag cgaccgggcg tcgttcgtga cgggagcggt gctgcccgtg
     4861 gacggcggat tcacgatggt gtgacgctgc gcggcggtgt gacacgggcg gagcccggcg
     4921 gcctgatccg gctgccgggc tccgcccgtg ttccgctgtg gtgcgtcttg ccccgccttg
     4981 cgtcgcttgc ccccacctcg tcaggcgacg ggcgcgggag cggtgtgcgt ggtggtgaag
     5041 acgggctggt cgttctggag cccgacggtc gtgtgggtga cggtgtgcga ggtagtacgg
     5101 gtctcggtcg tgagggtgat gggggcgtcg aactccgcgt accggtggaa cgtggtgtgc
     5161 gtggcggtgg gctggggccg gtagccggtg cccgcggcgt ccggggcgca ggcgcacgga
     5221 tccacgtccg tggccgcgtc cgtgcctgcg gtgacggcct cggtgacggg tgcggtgacg
     5281 gtgtacgcgc cctggttggc ggcctcgatg agcacgagtc cggggacgtg gtcgacggcg
     5341 tggtcgtaga ggacggtgtt gtcgaaggtg tggcgcagct gccaggtgcg gggctgcgcc
     5401 gtggggctga gcacgacctc gtcgggcgtg gtgcggccga cctgcacggg cgggagcggc
     5461 ggcgtgacgg gcggcagggt cgtgggcggc cggtggtacg caccccggag gcggcggtag
     5521 accatggggg gaatccactc gaagtcggtc tcgcccgtgg cgagaacggt ctcggaggcc
     5581 tcgtcgccgg cgtcgccgga cccggcggcg gtgacggtca tgtggaggtg gagggcggtg
     5641 gtgcgcttgt tgcgcgtcgt gggggtggcc gtgatgttga cgtgcagcgg cgtcgcccgg
     5701 tgggtgtcga cggcggcgtc ggggttgagg gtgtaggaga aggtgcgcag gagagtggcg
     5761 tggtccagcg gggccgcgta ggcggtgtgg gcgaggaaga gaccggcctg gcgaatggtc
     5821 tgggcgacgg tgagggtctc gtagtgggtg ccgtggccgg gtatgcggtg ggccgtgtgg
     5881 gcgggccaga ccgcggagac ggtgtaatgg gtggcgtcct gctgctcgca gccggtcagg
     5941 tacacggcgt cgtcgaagct caggtgcacc agctccttgg cgacgcggcc gctgccgccg
     6001 gccccggagc cgtcggcggt gtcgccggca ccgttaccgg cccgcgagtc ctcggcgcta
     6061 aggtctgcgg ctgaggcgcg gagccggccg gacgcatcga tccgatcggc ctgcagccgg
     6121 acgttccggc acgggacgga ccggtcggcg tgctgctggg tgctctggtc ggcacgggga
     6181 gcggggctga gcaggatctg agacacgtct ctcctcgaag tggattcgtg cgtcccggtg
     6241 gttagccggc tcggtgataa gatacgtact atccggtttg ctttatgggt gggtggcagg
     6301 gtatggagcc ggccgcccgg cgggccgggt gaggaagacc ccggattcgc cgcaccgggg
     6361 gcggaccgca ggttccgccg gtgcgccggt gtcgtcgtgc ttgctcaggc cgttgtccgc
     6421 gggccctgcc cggtcccgtc cggctgcgcc cggcgatcct gccgggtgag cggtcttgta
     6481 gcggttggct gtcggtgatt gggaggtaat caatggcgaa gcaggcgcgg gcgattcaca
     6541 cgcgccgtct cattctcgag gcggcgggcg aggtcttcga cgaatacgga tacgagtcgg
     6601 cgacgatcgc ggagatcctc gggcgcgcgg gcgtgaccaa gggtgcgctg tacttccact
     6661 tcgcctccaa ggaggagctg gcgcgcggcg tcctgagcga ggcgttgacg accgaaggcg
     6721 tcatgcccca ggagtcgcgc ctgcaggagt gggtggacgt cggcatggtg ctggcgcacc
     6781 ggctgcccag ggagcccctg ctgagcgccg cgatccggct gtcggccgac cgcaaggcgc
     6841 gggacctgtt cggcacgtcc tggccggggt ggatctcctt catcgcgggg cagctggccg
     6901 cggcgaagga gcagggggag ctgctgcccc atgtcgacac ggaccggatc gccgtgctgt
     6961 tcgtgagcgc ctggacgggc gtcgaggtcc tctcgacggc gctggacggg ggcgactccc
     7021 tggaggagcg ggtcgcgctg ctctacgaca cgatcctgcc gggcatcgcg gtgccgggtg
     7081 cactgcgggc gctggacacg gcccccgacc gcgggacgcg ggtctgggac gcgcaccggg
     7141 cccagcgcga gcaggaagcg gcgtccgccg cgtcgtgacg accgaggcgt tcccccggcg
     7201 gtgaggccgg gcgggcagga gatgtgccgg cggcgggctg tgcaggaccg ctgccgcatg
     7261 gaggacaggc tctccgcgct gccacggatg acgacgtcca cgcttccccc tgtgtttgtg
     7321 gccgatgccg gcgcgggcgc ccggtgccga tggacacgag cccccctcgt tcctcccggc
     7381 acggcatcgg aaagctgtcc cggagccgct gctcgtgacg ggcggggcct cgggtgatcc
     7441 gacgtcaggg acgctaacaa accggccatc ctgtttgtca aggctcgacg ggaatcccat
     7501 actcatcccg tgacctgggt gaatggtcca ccgggaaggc ggccgggcgc agccgaagga
     7561 gaaaccgcac ggttggtttc tccatgcttt caagggaacg acaaaggccc gcaatgtgca
     7621 ttgcgggcct ggtggtcggt tgcgggggcg cggctgccgt tctccgtccg acgtggcgct
     7681 gcgggagctc agccgcggag cgcctcggcc ccggcgggcc ggtcggcggt ggtgccggtc
     7741 gcgttgatga cggattccgt gccggcgggc tgcagttggg tcagtgcctt gggcgttgcc
     7801 agctgcggca gcagagtgtg ccagaggccg gtgagggtct ggcgtgagag ccagctgcgg
     7861 tcgtgacgtg ccagggcctc gaatccggcg gtcgccccca cgaccgcggc ggtcagctcc
     7921 tcccgcacgg cctgcggcgg tgtctgcccg tgggtcgcgg accgttccag cgtgcggcgg
     7981 acgcactcga gccactcctg gcgcagattc gtctcggagt ggtgcgcggt acggcagttg
     8041 aggtggaaac ccgcgcgcac cacgacgtcc caccgcagct gctgggcgat gacgtgcgac
     8101 gagtcgacga gcgcctgcag gggggagggg ctcttgcggt gggcgtgccg ggccagggcg
     8161 tgcagggtcc gggcggcttc ggactccacg gccgcggcca tggccgcctt ggtgtcgaag
     8221 tggaagtgca gggcgcccga gctcaccccg gcgccggcgc tgatgccggc gagcttggcg
     8281 cgggtgtagc cgaactggtc gaaggcctgg gctgcggagc ggatcaatgc gtgccgggtg
     8341 ttcatggccc gttcttgctt cgtcaccggc aactcctggg ggacaacgtc atggggcacg
     8401 tcatgggcca catgaaacca actaactggt tttatttcta ctgccactgc ccggtatagg
     8461 cgttaggtga ggtatctggc gtccaggggt ccgtacaggc cgtacctctc gggttgcaca
     8521 ccgtgccgac cgggggagtg acgtaagggg gtcgccttct cgtggatgcc ggtaaatcct
     8581 cttactgaag ggcaagttgc ctcaaccttt cagcctgcgc cgttcgctcg tgaccgaaat
     8641 gcggcttttg atgcgagagg cgacgtagca cccgattagt tctttgacaa accgacagcc
     8701 cggtttgtaa tgtcgcggtg aacggttgtt cgcggcatcg tcgccgcgcg aacgtgcagg
     8761 agcagcgatg gatcagcagg tcagcaccct tgtcgatgcg cacgccgggg aagcgtcccg
     8821 gcgtcgtgtg cccgggccga gggccgggca ccctgcagac cgggccccgg ccctgacgcg
     8881 gccgggcgag accctgcgga tcctcgtcgt cgacagcgac acccagtccg ccgagacgct
     8941 ttcccggaga ctgcagcgcc acggctgcga gaccttctcc gtcgccaccg gggaggccgc
     9001 cctcgccgag taccgttcgg cggacctggt cctgctggat ccggaactgc ccgatctgga
     9061 cggcctggaa gtctgccggg ccctgagagc cgccagcgag gtccccatca tcgccgtgac
     9121 cgccctggcc ggcgagctcg accgggtcct cggtctgcag gccggcgccg acgactacgt
     9181 cgtcgagccc tacggcttcc gcgagctgat ggcccgtatc gaagccgtca tgcgccgtgc
     9241 gcgccccgca gcgcgcacct cggcgggggt catcaccagc ggagcgctga ccatcgaccc
     9301 cgacctgcgc gaagtgcgcc tggggggacg ccggatcgac gtcacccgca aggaattcga
     9361 cctgctccac ctgctggcgt cccgccccga gaaggtcgtc tcccgcgagc agctcatgac
     9421 ccaggtgtgg gacgactact ggtcccgccc cggccggacg atcgacaccc acgtcagcag
     9481 cctgcggggc aagctcgggt ccgcaggctg gatcatcacc atccgcggcg tcggcttccg
     9541 cttcgggcgc gcctgagccc gcacacgccc cctccctcgc cgtcgactcg tcgatcaccc
     9601 ctcgaccgct ctctgattgg gtctgccgac atccgatgac ttccgatcgt ctttgaaaga
     9661 ggacattcat cgtgcgcaag gtgctcatcg ccaaccgtgg cgaaatcgct gtccgcgtag
     9721 ctcgcgcgtg ccgggatgcc gggattgcca gtgtggcggt ctacgcggat ccggaccggg
     9781 acgcgctgca tgtccgcgct gctgatgagg cgtatgcgct gggcggtgac accccggcga
     9841 cgagctacct cgatgtggcc aaggtgcttg ccgcggcggc cgagtcgggg gcggatgccg
     9901 ttcaccccgg gtacggcttc ctttccgaga acgccgagtt cgcgcaggcc gtgctggatg
     9961 cgggtctgac gtggatcggt ccgccgccgc aggcgatccg ggatctgggt gacaaggtgg
    10021 cggcccgtca catcgcccag cgtgcgggtg cgccgctggt ggccggtacc gccgacccgg
    10081 ttgcgggggc ggaggaggtc gtggcgttcg cgcaggaaca cggcctgccg atcgcgatca
    10141 aggccgcttt cggtggtggc gggcgtggtc tgaaggtcgc ccgcacgctg gaggaggtcc
    10201 ccgagctcta cgactccgcc gtccgcgagg cggtcgcggc gttcgggcgg ggggagtgct
    10261 tcgtcgagcg ctacctggac cggccccgtc acgtcgagac gcagtgcctg gccgacaagc
    10321 acggcaacgt ggtggtcgtc tccacgcgtg actgctcgct gcagcgccgc caccagaagc
    10381 tggtggagga ggctccggcg ccgttcctgt cggcggagca gaacgccgag ctctaccgtg
    10441 cctcgaaggc gatcctgcgc gaggccggtt acgagggcgc gggcacgtgc gagttcctcg
    10501 tcggccagga cggcacgatc tccttcctgg aggtcaacac ccgtctgcag gtcgagcacc
    10561 cggtcaccga agaggtcacc ggcatcgacc tggtccgcga gatgttccgc atcgccgacg
    10621 gcgaggagct cggctacgac gacccgccgc tgcgtggtca ctcgttcgag ttccgcatca
    10681 acggtgagga cccgggccgc aacttcctgc ccgctccggg cacggtgacc cgcttcgatg
    10741 cgccgaccgg tccgggtgtg cgcctggatg cgggtgtgga gtcgggcagt gtgatcggcc
    10801 cggcgtggga ctcgctgctg gcgaagctga tcgtcaccgg tgccacgcgt gagcaggccc
    10861 tgcagcgggc ggcgcgggca ctgaacgagt tcaaggtgga aggcatggcc acggccatcc
    10921 ccttccacca ggccgtggtg accgaccccg ccttcaccgc cgaccccttc cgggtccaca
    10981 cgcggtggat cgagaccgag ttcgtcaaca ccatcgagcc gttcgccgga gcggcggccg
    11041 aggaagaggg cgaggaagcc ggccgcgaga ccgtcgtcgt cgaggtcggc ggcaagcgcc
    11101 tggaagtctc cctcccctcc tccctcggca tggccatcgc ccgcaccact gcgcccggcg
    11161 gggccaagcc caagcgccgc gccgccaaga agtccggctt cgcggt
//
