LOCUS       NZ_LNBE01000002        21308 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_2, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000002
VERSION     NZ_LNBE01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1142035)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 1142035)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 1142035)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 700246
            Orig. end                         :: 721554
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21308
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="11"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21308
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21308
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11308](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11308
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    197..206
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            722..1051
                     /locus_tag="AS857_RS04680"
     CDS             722..1051
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931259.1"
                     /locus_tag="AS857_RS04680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058042101.1"
                     /transl_table=11
                     /translation="MALPRKGSRRIVVDGVAYRWRIRRRVTYSQGMGWSPLAYAVEHAQ
                     SPGTTLVVRTGRPHPGNWISRPASPVLPAHVSAAVRAARARGWTPEAPGPPFLLDEPGG
                     TVASP"
     gene            complement(1363..1494)
                     /locus_tag="AS857_RS41805"
     CDS             complement(1363..1494)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS41805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_275477345.1"
                     /transl_table=11
                     /translation="MGIAELGALPAALLGPAPVYVHACGGAHLQYAVQFGDPEIRCA"
     gene            1500..1778
                     /locus_tag="AS857_RS04685"
     CDS             1500..1778
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS04685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058041814.1"
                     /transl_table=11
                     /translation="MAQRLHGRPDSGTREQRPGRTGARPAPPVPALVSGPGPRLADVEP
                     PLVGRANWWTPAILRRREPKSGTDGTDGTDGTDGTADAAGPMDPTTA"
     gene            1983..2519
                     /locus_tag="AS857_RS04690"
     CDS             1983..2519
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS04690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="BCD family MFS transporter"
                     /protein_id="WP_144440717.1"
                     /transl_table=11
                     /translation="MLLTPALLTAVSWLNPPDDTLLLFAGFALMVIILIAAWCMCGPGP
                     GAGMAALGFSFVLFTGPALDDYVMEQRGTTHEALVGDVSTYTRKHGNEGRTCSVVWSAS
                     GETHRAEVGDRKGCEESFREGDRTTIVTDPEGWLRPRLGSEVTGLSSGTAWTCGGLLAG
                     MEAFILYGRLRRRRA"
     gene            2624..3079
                     /locus_tag="AS857_RS04695"
     CDS             2624..3079
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240763.1"
                     /locus_tag="AS857_RS04695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_107105496.1"
                     /transl_table=11
                     /translation="MNGTFEVDTGDGCPPSGDASGRAAAVRAAMDGLLQIRRLMNGHLT
                     DPASGPADWERRQPVRAVALVLEAAGIQPSALDADGQRSATGYCVRPGEQPGSVRVEWL
                     GPPGSGAAAQQHEALQRCARVLEDLGWPALEYRGARRQRWLEAEPVT"
     gene            complement(3179..6697)
                     /locus_tag="AS857_RS04700"
     CDS             complement(3179..6697)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984306.1"
                     /locus_tag="AS857_RS04700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_245699578.1"
                     /transl_table=11
                     /translation="MSARHDGHDLPIGPPRRRALLALLLIRPGRVVPTELLVEELWGEE
                     PPRRAVATLQSHVSHLRRALQPTAGPDGASVLRHRAGGYVLELDPARIDVCRFELLVTE
                     GRRLLEQREPLAARDRLAQALALWRGTPYGEFDGHPPLSDESTRLEHVRLTAVESCAEA
                     RLALGAAEEVAADLEQEARRHPARERLVGHLMTALSRLGRQAEALEVYERTRSHLAEEF
                     GVGTAAALRRVRTAILRQEPGTGSPTEESRHGHADPVVRGAEDAAADAVGAVDATDVAA
                     LTALAAVAAVVAVPGGTVPEDDTVRVVQKAGTAAATDGGGAAGAAETGGSAGAAGATRT
                     AAATHTADTVDTTSTSDTSDTTDTSDTTEPGLPMGQSPFTGRSEELQRLTAAATSALSG
                     HGHVAGVLGPAGVGKTRLLLELAPRLEAVCASRGGADLEVIWSHCFLSEGVPPYWLWTQ
                     VLRRLSVTRPDAFRRAAEPFGSLLAPLMPERSAGPGGGGPGADSDWGQARFLTHDAVCE
                     VLLTLAGQRPLVLFLEDLHWADTASLDLLRLLSTRRQGHRLGIVLTAREFEVEADATMR
                     RMLSEILRGPRTETLRLDGLSRQAVAAIVVAQAGPGVDAKVVEVLHRRSEGNPYFVMQL
                     LSLLGDPRSLHRPDAADVLLTRVPTGVREVLHQRFAALPEPVLQVLRLCAVIGSEVDTD
                     LLHRTAAADEPVAGALGSAIRAGLLGEDAHHPERIHFAHALVQETLIEELAREDRQRLH
                     ARVAEALCARRHGQVGDGDVEQVAHHTWHAGNALPPAQALPRLLRAADQAEQQLAYERV
                     ETWLRRAAHLVSLLPHDDPSALSLEQRLNIQLGQVLATTRGYGHPEAETALAHGRALSS
                     ATEAPEDPSVLWALCAAYLVTGRYDASRRFSGLLRGIAERTREPVAVLGAAYGEGIVLH
                     IRGRLPQALAELEHGVDMADRYAREGHALARTFQHDPRVSCRSYNTFTHWLLGDRRTAA
                     ARRRELLHLTEYDSRPSDRSFALYVDAVVAAWEGDARTARSSGAEGARLAGEHGLLYWK
                     GMLGLLEGWGLTHSGWEGDGLALMHASLAELRRSSTLLRHPLHLGLLGEAQHHAGRPEE
                     ASETFRALLAAVERRGERVYLHEALPATRLLHDLLGSDAAGAAAGAKPPHARWASRRPP
                     GGLAAI"
     gene            7258..8088
                     /locus_tag="AS857_RS04705"
     CDS             7258..8088
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: oMT (31-175). E-value: 3.4e-44. Score:
                     142.6. Matches aSDomain:
                     nrpspksdomains_AS857_RS04705_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140915.1"
                     /locus_tag="AS857_RS04705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_058041816.1"
                     /sec_met_domain="PCMT (E-value: 1.2e-05, bitscore: 20.9,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSAHTVFPVPEEVGRLYDRLTALDTGTVGGSLHLGYWDAEDDGV
                     PLVEAADRLTDMMTDRLHVDESHRVLDVGCGVGQPAMRIARRTGAHVTGISVSRAQVAR
                     ATALAGAAGLGDRVEFRYADAMRLPFEDNSFDAAIAIESIFHMPDREQVFREIRRVLRP
                     GGRLVLTDFFERAPIPAGLRSAMDRCLRDFIMTLARPEDYVPMLSRAGLCFTELLDITE
                     QCVRRTFEQMSRSRRGQGELQTVFDEAAGEKFGPADMIDFDEFGSVLLAAQKPA"
     aSDomain        7351..7782
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS04705_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="3.40E-44"
                     /label="AS857_RS04705_oMT.1"
                     /locus_tag="AS857_RS04705"
                     /protein_end="175"
                     /protein_start="31"
                     /score="142.6"
                     /tool="antismash"
                     /translation="SLHLGYWDAEDDGVPLVEAADRLTDMMTDRLHVDESHRVLDVGCG
                     VGQPAMRIARRTGAHVTGISVSRAQVARATALAGAAGLGDRVEFRYADAMRLPFEDNSF
                     DAAIAIESIFHMPDREQVFREIRRVLRPGGRLVLTDFFER"
     misc_feature    8297..8311
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.58"
                     /tool="antismash"
     gene            8538..9965
                     /locus_tag="AS857_RS04710"
     CDS             8538..9965
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538392.1"
                     /locus_tag="AS857_RS04710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 2B encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_058041817.1"
                     /transl_table=11
                     /translation="MSTAAHWQQPEQPEQQAGRAQLSLGIAAARNLASTTKTVPQMQGI
                     SSRWLLRMLPWVEVKGGTYRVNRRLVYEVGDGRVTFVQEGAAVKVVPAELGELPLLRGF
                     HDDEALRALAERCRQQEYEPGQVIAERGRPVDHVYLIARGKVEKAGTGKYGEETRRGVL
                     ADGGFFGAQTVAEEPGKWEFTARAMTACTVLALPRYAYEEVAGRHEELRAHVRRRQAEK
                     ARPQNKKGEANITLASGHTGEPALPGTFVDYELAPREYELSVAQTILRVHTRVADLYND
                     PMDQVEHQLRLTIEALRERQESELVNNPEFGLLHNADYSQRISTHSGPPTPDDMDELLS
                     MRRGTKAFFAHPRAISALARECNKRGLSFDSADVNGHPVPAWRGVPILPCGKIPVSEEG
                     TSSILAMRLGEGEQGVVGLRQTGIPDEYEPGLNVRFMGINDQALISYLVSTYYSAAVLV
                     PDALGVLENVEVFHTPS"
     gene            10001..11308
                     /locus_tag="AS857_RS04715"
     CDS             10001..11308
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890277.1"
                     /locus_tag="AS857_RS04715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 2 encapsulin nanocompartment cargo protein
                     terpene cyclase"
                     /protein_id="WP_058041818.1"
                     /sec_met_domain="T1TS (E-value: 3.2e-57, bitscore: 190.5,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLLSRITAPPARHDVARLVATLLTHRQDARRSAPQALPGTTPRP
                     AGTALPTGPTGLGTSAARLAAGKARETWRAGKAGAAGEAGNVAGKPAAGAPGAPGISVA
                     PVASGAASDDRGTGVPELYCPPALRDDPALAREVNDRLVDWADEAGIYAGRLDRVRAAD
                     FGRLMMLAHPDSDDPDRLLAAGKCALAEWATDDYYCDDETMGSRTELLGSHLGLAYTAL
                     DPSHLPARYAPAWEEEMREDPVRVALRSGLDHLSRYAEPSQVARLRHEVAVLFVGYEQE
                     GSWRSQGRMPGVREYLAHRQINSFLPCIALADVVGGYALPASEYAEPRVRRAVTMAATA
                     ATLVNDLYSMTKEHDSSGVEFNLPVVIAEEDGCSLREAIERSAEIHDELVHTYETEAAA
                     LALTGTPELRRFLAGVWAWLGGNREWHAGSPRYNGA"
     gene            11452..12318
                     /locus_tag="AS857_RS04720"
     CDS             11452..12318
                     /EC_number="2.1.1.255"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0042214 - terpene metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538391.1"
                     /locus_tag="AS857_RS04720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="geranyl diphosphate 2-C-methyltransferase"
                     /protein_id="WP_058041819.1"
                     /transl_table=11
                     /translation="MTTTPAPAASAGVLRTAYQKSVAEYWNNEKDPVNLRLGDVDGLYH
                     HHYGLGDYDPSVLEGPEETRDQRIIEELHRLESAQADVLLDHLGPIAPDQRVLDAGCGR
                     GGTSIMANARFGCRADGVSISQAQVDFANEQARKRGVDDKVRYHFANMLDTGLPTGDFQ
                     GIWNNESTMYVDLNELFAEHARQLAYGGRYVTITGCSNDVTGLRSKAVSRIDEHYTCNI
                     HPRSAYFKAMAANGLVPINVVDLTAATIPYWELRAQSSLATGVEDPFLTAYKEGSFHYL
                     LIAADRI"
     gene            complement(12399..13172)
                     /locus_tag="AS857_RS04725"
     CDS             complement(12399..13172)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643916.1"
                     /locus_tag="AS857_RS04725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyltransferase domain-containing protein"
                     /protein_id="WP_058041820.1"
                     /transl_table=11
                     /translation="MTDDDNLFLAPIADRLAALPSVLAVTLGGSRAQSTHTPDSDWDLA
                     VYYRGGFDPAELRAVGWEGEVSEIGGWGGGVFNGGAWLTIDGRHVDVHYRDLDVVEHEL
                     AEARQGRFHWEPLMFHLAGIPSYLVVAELAVNQVLRGTLPRPEYPQALRDAAPPMWRER
                     AALTLRYAKGAYVRRGQAAEVAGALAVAAMQTGHAVLAGRGEWVTNEKRLLQQAGLRGI
                     DAIVAGLRPEPEALTRAITEAERLFERAHATTPEH"
     misc_feature    13601..13615
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.78"
                     /tool="antismash"
     gene            13610..>14017
                     /locus_tag="AS857_RS40980"
                     /pseudo=""
     CDS             13610..>14017
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006124619.1"
                     /locus_tag="AS857_RS40980"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF4157 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLRQADRPRTGEQHQQAAQPESQSSAVHEVLRSRGRPLADSIRSD
                     MENRLGADFSDVRIHDDSAAKASAAEVGARAYTSGSHVVIGDGGGDKRTLAHELTHVIQ
                     QRQGPVAGADNGSGLKVSDPSDRFEREAEANA"
     misc_feature    13620..13634
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 24.7"
                     /tool="antismash"
     gene            complement(14754..15557)
                     /locus_tag="AS857_RS04735"
     CDS             complement(14754..15557)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088938.1"
                     /locus_tag="AS857_RS04735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_058041822.1"
                     /transl_table=11
                     /translation="MNDTAPQQQPQQKKILLVTAHPEPRSLNASLAAFATEHLRAAGHE
                     VQVSDLYAMKWKAAVDADDFPDEPGDRRLQVMAASERATLAGRLSADIAAEQEKVRWSD
                     AVILQFPLWWFSMPAILKGWVDRVFTAGFGYGPTLPPPYSESALGGRRALVSVTAGARE
                     SSFSDRGIHGRLADVLHPVQHGLFWFTGMTPLEPFGVYRANDLPEERFEAAKREYAHRL
                     DGLFTDAPVPYRTLTGGDYDHDMRLLPGVETPGTSGLDLHVRPHS"
     gene            15656..16555
                     /locus_tag="AS857_RS04740"
     CDS             15656..16555
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179418.1"
                     /locus_tag="AS857_RS04740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_058041823.1"
                     /transl_table=11
                     /translation="MNGVDDSRIGGVDGVDGVGDAQRALGRFLRARRGRVAPEQVGLAG
                     GRRRRVRGLRREELAQLAGISVDYYVRLEQGRATQPSAEVLDALAGALGLDAAERKHLD
                     TLAGARRDPVPRAEVSPLLQRVLDSLDRFPVFVTDHRMDVVAWNALGAELIGGAPGPGS
                     RDRNNARYVFLDPASRAVHPDWEARAAEAVGQLRVAAGRYPDDAELSALINELSAHSAD
                     FRRIWATGEVVMCAAGRKRLRHPEAGLLTLDFETLHVPAAPGETGLVVHVFSAEEGSEE
                     ASALARLATAVTGVPVPA"
     gene            complement(16597..16944)
                     /locus_tag="AS857_RS04745"
     CDS             complement(16597..16944)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS04745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058041824.1"
                     /transl_table=11
                     /translation="MTDHVDDLLDPGNDWSRRMQDRLTGLSPELTELVLHLGTSADFWN
                     HRYKVDVAWKRRTKVLLKVGGAGELVRAARSLDWEYEIPDGLGFRPLGPGSASTSLPTT
                     ARVRLRPQKTR"
     gene            complement(16994..18025)
                     /locus_tag="AS857_RS04750"
     CDS             complement(16994..18025)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS04750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_144440718.1"
                     /transl_table=11
                     /translation="MNTSSVPRRSVLRALALAVTLVGCATSTWDGQAFAAAQQPETYRL
                     TIRHLDRVGSPTGAYDTTVTGISGPGSGESVNPHDTSGTTTVRLPKGRYLLDSYLTTGK
                     AAAGTGGADGTDGADWIVQPRLDLDRDTTVTVDARTTAPVDVRPPDRSAAFLHGMMVTE
                     VKHGGATRLVNRMVSTPNLRVAHLGPDAGPGSVKQWYDAYWTTKSADYALGYVFTGSRA
                     LTGLTRHPSAKDLATVQVRGGARPGTTGPATVDLQPSAGPTVGLSRRLTVPGTATYFVT
                     PERGTWDITYWSPTAPGTPSNRATADGIAVRAGSTTTHTFDGPVSGPAPARTDTSTGTH
                     RTR"
     misc_feature    18011..18025
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.48"
                     /tool="antismash"
     gene            18170..19045
                     /locus_tag="AS857_RS40985"
     CDS             18170..19045
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015032186.1"
                     /locus_tag="AS857_RS40985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_245699579.1"
                     /transl_table=11
                     /translation="MRNRFRRGDRDALAELYDEHAQVLYRYALRVTGDWAEAEDVVSAT
                     FLEAWRGRERVHPDGDSLRPWLLGIATNIMRRNARARRLRDIALARVPERGSIPDFADD
                     LVTHLADTEQLRAAHAALARLRRRDREVFTLVVWAGLDYAAAAEALGVPVGTVRSRLSR
                     ARTRLRELADAEARAVRSRGSHTSKASHISDTSEIRRAEAGRPRAAVGDEAAAAAFDEP
                     LAKPLDAPLAAPLGPPRGKSLGKPVIRSLAERLDRPHAEPLAKAPAPPLDQSAPGRIPA
                     PRSSTQENHG"
     gene            19042..20172
                     /locus_tag="AS857_RS04760"
     CDS             19042..20172
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962685.1"
                     /locus_tag="AS857_RS04760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CU044_5270 family protein"
                     /protein_id="WP_058041826.1"
                     /transl_table=11
                     /translation="MNTTPRRRPLPGSEDTERLGLADLLPPPPVPGLDPDRELLLKEAL
                     LREAAADAPRGSLAARASRLRPSRRLVVRVMAPAVACALAVGGVVAVNLADTPGRGVPT
                     VPERDGADRTPEATRLLDRIALAAAAAPQPTVRAGQFVYVESKVAYASQSAAGGAVTMA
                     PVHTRQVWLSADGSRPGLLREEGRPDAPLGADDTPVYALDGPGATPRPTTLHKGPSISD
                     PTHQYVASLPTDPDALLKLIRDETRGGSQDRDRDRDSDPDQRAFAAIGDLLAETWAPPK
                     VSAALYKAAAKIPGVTVVRSAADAAERAGIAVARTAHGQQTQWVFDRSTYAFLGERTVL
                     TEAGDAGPAGTVVGASAVLAKAAVDRVGVIPASSMK"
     misc_feature    20320..20338
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.83"
                     /tool="antismash"
     misc_feature    20802..20816
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
ORIGIN
        1 ccgtggagaa accctgcacc agacggcaga tgaggagcag gacgggggcg acgaagccgg
       61 ccgtcgcgta gaccggcagg aaaccgacgg cgaacgtgct caccgccatc atgatcatcg
      121 tggcggcgag cacgcgctga cgtccgatgc ggtcgccgag cgggccgaag accaggccgc
      181 cgaggggccg gacgaggaag gcggccgcga aggtggcgaa ggcggagacg acctgtgcgc
      241 cgggcgaggt ggaagggaag aagacccggc ccagggtgac cgccatatag gcgtacaccc
      301 cgaagtcgaa ccactccatc atgttgccga gtgcggcggc gagcaccgcg cggcggacct
      361 cggggcggtc ggtgaccgtg acgtcctcga tgttcaggga cttcttgcgg cgcctcagca
      421 gggtgcggag catccggtcg gtggcggtcg cgccaccgcg cgcagggacg cggtgcccgg
      481 gcttcggggt cttcttgcga tcggggctca cactcacctg ccggcgggtc ggggtccggg
      541 cgctggggct cctggggcgt acagagacgc atgagccgaa ttttgtggtt tatgcattga
      601 acctcgcccg gcccccggca gccccgtgac ccgcccggcc cgcgccgaac cgaccccgat
      661 gcgttctgcc gggagccggc agctgttcgt taccctttcg gccctgagga aggggacgcg
      721 cttggcactg cccaggaagg ggtcgcgtcg catcgtcgtc gacggtgtcg cctaccggtg
      781 gaggatccgg aggagggtca cctacagcca agggatgggc tggtcgcccc tggcctatgc
      841 ggtcgagcac gcccagtcgc cgggcaccac gctcgtggtc cggacgggcc ggccgcaccc
      901 cggcaactgg atcagcaggc cggcctctcc cgttctcccc gcccacgtct ccgccgccgt
      961 ccgggccgcc cgcgcacgcg gctggacgcc ggaggctccc gggccgccct ttctcctgga
     1021 cgagcccggg ggcacggtcg cgtctccctg accatccacc acccccgtcg gcgactgtcg
     1081 gcgaccgtcg gcgaccgccg cgtcaacgca cgccggacga gcctcctcgg ttgagtggct
     1141 tgcccggtga cgaggcctgt gcccgcaatg cgcccgggcg gcaactgctg cggtgcgtac
     1201 ggtgtttggg gcgcagcgtc atccaggccg caccgcgtcg gagccggccc ggagccggcg
     1261 cagttcgcgg cggccgagcg cctcgccggg gagctgaccg ggcgcggcgg gccgctccgg
     1321 gtgaccgccg ccggtgatta gccgcgaggc ggacctggac cgctacgcgc accggatctc
     1381 cggatctccg aactggacgg cgtactgcag gtgcgcaccg ccgcaggcgt gcacgtacac
     1441 gggggcgggt cccagcagag ccgcagggag ggcgccgagt tcggcaatcc ccacggcacg
     1501 tggctcagcg tctccacggc cgaccggact ccgggacccg ggagcagcgc cctggccgga
     1561 cgggtgcgcg ccctgcccca cccgtccccg ccctggtcag cggccccggg ccccggctcg
     1621 ccgatgtcga acctccgctg gtcggccggg cgaactggtg gacgcctgcg atcctccggc
     1681 ggcgcgagcc gaagtccggc acggacggca cggacggcac ggacggtacg gacggtacgg
     1741 ccgacgcggc cgggccgatg gaccccacca ccgcgtaacc tcgccggtcg gggtcctcca
     1801 acggggctcc ccgtacggtg cagaagagat cgaggcgtgg tgagctcgtg gtgagcaaca
     1861 gggcggatgc ggatgcggat accggcatac gcggacgggt gcggggtcag gggccggcag
     1921 acccgggtgc gacggccgcc gggcgcggcg gcaggcccag gtggccgggc acggtggcca
     1981 cgatgctgct cacaccggcg ctcctgactg ccgtctcgtg gctgaacccg cccgacgaca
     2041 cgctgctgtt gttcgccggg ttcgccctga tggtgatcat cctgatcgcg gcctggtgca
     2101 tgtgcggccc gggaccgggc gccggcatgg cggccctcgg cttctccttc gtcctcttca
     2161 ccggcccggc cctggacgac tacgtcatgg aacagcgcgg caccacccac gaggcgctgg
     2221 tcggcgacgt ctccacgtac acccggaaac acggcaatga aggccgcacc tgctcggtcg
     2281 tgtggtccgc ttcgggggag acgcacaggg ccgaggtggg cgaccgcaag ggctgcgagg
     2341 agagcttccg ggagggcgac aggaccacga tcgtcacgga cccggaaggc tggctgaggc
     2401 cgcgcctggg cagcgaggtc acggggctgt cgtcgggcac ggcctggacc tgcgggggcc
     2461 tgctggccgg aatggaggcg ttcatcctgt acggccgcct gaggcgccgc cgcgcctgac
     2521 gcccgtacgc gcggtgttgt cagtggctgc gcccatagtg ggtctccggg caggcagccg
     2581 gacagcaggc gacggccgga tcgatgggca aggaggcagc cgagtgaacg ggacgttcga
     2641 ggtggacacc ggagacgggt gcccgccgtc gggggacgct tccgggcggg ccgcggcggt
     2701 gcgcgcggcg atggacgggc tgctgcagat ccggcggctg atgaacggcc acctcacgga
     2761 cccggcatcc ggccccgccg actgggaacg gcgtcagccg gtgcgtgccg tcgccctcgt
     2821 cctcgaagcc gccggcatcc agccctccgc tctcgacgcc gacgggcagc gctccgcgac
     2881 cggctactgc gtccgtcccg gggaacagcc ggggagcgta cgggtcgagt ggctcggccc
     2941 gccggggagc ggggccgccg cccagcagca cgaggccctc cagcgctgcg cccgcgttct
     3001 ggaggacctg gggtggccgg cgctggaata ccgcggggca cgccggcagc gctggctcga
     3061 agccgagccc gtcacgtagc gaggccgcac gcgggcgtac ggccttgtgg accgctgcaa
     3121 gggtgcaggc cgggcacgcc tgccccggcc cgatgacgtc cccggcgggc cgcgtgcgtc
     3181 agatggcggc gaggccgccc ggaggccttc gggacgccca ccgggcatgg ggaggcttcg
     3241 cccccgcagc cgcccccgcc gcatcgctcc ccaggagatc gtgcagcagc cgggtggcgg
     3301 gcagggcctc gtgcagatag acccgctccc cgcgccgctc gacggccgcg agcagcgcgc
     3361 ggaacgtctc gctcgcctcc tcgggccgcc ccgcgtggtg ctgggcctcg ccgagcaggc
     3421 ccaggtggag ggggtggcgc aagagcgtgc tggagcggcg cagttcggcc agggaggcgt
     3481 gcatcagggc gaggccgtcg ccctcccacc ccgagtgcgt caggccccac ccttcgagca
     3541 ggcccagcat ccccttccag tagagcagcc cgtgttcgcc cgccaggcgg gcgccctcgg
     3601 cgccggagga ccgggccgta cgggcgtcgc cctcccaggc cgccacgacc gcgtccacat
     3661 agagggcgaa ggaccggtcg gagggcctgc tgtcgtactc ggtcaggtgc agcagctcgc
     3721 gccggcgcgc cgccgcggtc cgccggtcgc cgaggagcca gtgggtgaac gtgttgtacg
     3781 agcggcagga gacgcgcggg tcgtgctgga aggtgcgggc cagcgcatgg ccttcgcggg
     3841 cgtagcggtc cgccatgtcg accccgtgct ccagttcggc gagcgcctgc ggcagccgcc
     3901 cgcggatgtg cagcacgatg ccttcgccgt acgccgcgcc gagcaccgcc acgggctccc
     3961 gggtccgttc cgcgatgccc cgcagcaggc cggagaaccg ccgtgaggcg tcgtagcggc
     4021 ccgtcaccaa atacgccgcg cacagcgccc agagcaccga cgggtcctcg ggcgcctcgg
     4081 tggccgagct gagggcccgg ccgtgcgcga gcgccgtctc ggcctcggga tggccgtagc
     4141 cgcgggtcgt ggccagcacc tgcccgagct ggatgttcag ccgctgttcc agggacaggg
     4201 ccgaggggtc gtcgtggggc agcagactga cgaggtgcgc cgcccggcgc agccacgtct
     4261 ccacgcgctc gtacgccagt tgctgctcgg cctggtcggc ggcgcgcagc agccgcggga
     4321 gagcctgcgc gggcggcagc gcattcccgg cgtgccaggt gtggtgggcc acctgctcga
     4381 cgtccccgtc ccccacctgc ccgtgcctgc gggcgcacag cgcctcggcg accctggcgt
     4441 gcagccgctg ccggtcctcg cgcgccagct cctcgatgag cgtctcctgg accagggcgt
     4501 gggcgaaatg gatccgttcc ggatggtgcg catcctcccc gagcagcccg gcgcggatcg
     4561 ccgaccccag cgccccggcg accggttcgt ccgctgcggc cgtgcggtgc agcaggtcgg
     4621 tgtcgacctc ggagccgatg acggcgcaca gccgcagcac ctgcagcacc ggttcgggca
     4681 gggccgcgaa ccgctggtgc agcacctcgc gcacccccgt cggaacgcgc gtcagcagta
     4741 cgtccgccgc gtcggggcgg tgcaggctgc gggggtcccc caggagggac aggagctgca
     4801 tgacgaagta cgggttgccc tcgctgcgcc ggtgcagcac ctcgacgacc ttcgcgtcca
     4861 cgcccggccc ggcctgcgcc acgacgatgg cggcgacggc ctgccgggag agcccgtcga
     4921 gccgcagggt ctcggtcctg gggccgcgca ggatctcgga gagcatccgg cgcatcgtgg
     4981 cgtcggcctc gacctcgaac tcgcgggcgg tcagcacgat gccgagccgg tggccctggc
     5041 ggcgcgtgct cagcagccgg agcaggtcga gcgaggcggt gtcggcccag tgcaggtctt
     5101 ccaggaagag gacgagcgga cgctgcccgg ccagggtgag caggacctcg cagaccgcgt
     5161 cgtgagtgag gaaccgggcc tgcccccagt cggaatcggc gcccggcccg ccgccaccgg
     5221 gcccggccga ccgctcgggc atgagcgggg ccaggagcga gccgaaaggt tccgccgcgc
     5281 ggcggaaggc gtccgggcgg gtgacggaca gccgccggag gacctgcgtc cacagccagt
     5341 agggcggtac gccctcgctg aggaaacagt ggctccagat cacttccagg tcggcgccgc
     5401 cccggctcgc gcagacggcc tccagccgtg gggcgagttc gaggagcagc cgggtcttgc
     5461 cgactccggc ggggccgagg acaccggcca cgtgcccgtg cccggacagc gcactcgtcg
     5521 ccgcggccgt cagccgctgc aactcctcgc tgcggccggt gaacggcgac tggcccatcg
     5581 ggagcccggg ctcggtggtg tctgaggtgt cggtggtgtc ggaggtgtcg gaggtgctgg
     5641 tcgtgtcgac ggtgtcggct gtatgcgttg ctgcggctgt acgtgttgct ccggctgctc
     5701 cggcgcttcc gcctgtttcg gccgctccgg ccgctccgcc gccgtcggtg gccgcggccg
     5761 tgccggcctt ctgtacgacg cgaacggtgt cgtcctccgg gacggttccg ccgggcacgg
     5821 cgacgacagc ggcgacagcg gcgagggcgg tgagggcggc gacatcagtc gcatcaactg
     5881 caccaaccgc atcggccgcg gcgtcctccg cgccgcggac cacaggatcg gcgtgcccgt
     5941 ggcgcgattc ctcagtaggg ctccccgttc ccggctcttg gcggagtatc gccgttcgga
     6001 cgcggcggag cgcggcggcc gtgcccacgc cgaactcctc ggccaggtga ctgcgcgtcc
     6061 gttcgtagac ctcgagggct tcggcttgcc ggccgagccg ggacaaagcg gtcatcagat
     6121 ggccgacgag gcgctcccgc gccggatggc ggcgcgcttc ctgttcgagg tccgcggcca
     6181 cctcttcggc cgctccgagg gccagccggg cctccgcgca ggactccacg gccgtgagcc
     6241 ggacgtgttc cagccgtgtg ctctcgtcgc tgagcggcgg atggccgtcg aactccccgt
     6301 agggcgtacc ccgccacagc gcgagcgcct gcgcgagccg gtcccgcgcg gcgagcggtt
     6361 cgcgctgctc aagcagccgc cgcccttcgg tgaccaggag ctcgaagcgg cagacgtcga
     6421 tccgtgcggg gtcgagctcc aggacgtagc cgcccgcccg gtggcggagc accgacgcgc
     6481 cgtccggccc ggccgtcggc tggagggcgc ggcgcaggtg ggagacgtgg ctctggagcg
     6541 tggcgacggc ccggcgcggc ggttcctcgc cccacagctc ctcgacgagc agttccgtgg
     6601 gcaccacgcg gccggggcgt atgaggagca gggcgagcag ggcccgcctg cgcggggggc
     6661 cgatcgggag gtcgtgcccg tcgtggcgtg ccgacatggg gcccagaacg ctcaggaggg
     6721 gttcgctcgg gtcggtggcc gccggcccgg ctgccggcgg ggctgtcggt gtcggctccg
     6781 gcgtcggaga tgcgggcatg tcggtcccaa tcgttctcgc acgcgcgact gatgacgggc
     6841 gtccggcgca gagaggccgg gccttacaaa acggatcttg gcatacgcaa cagggcaatc
     6901 caagatcacg acaagccatc ggcaagcgca tggcaagcga acggcgaggg tactgcaggg
     6961 agttcgccaa ggcggccgaa accggccgcc ggggtcggca aggaaacgtc aatggcacga
     7021 ccaggctgcc gcaagggcgg tggcgcacga tgtccgtcac tggaagagcc actgctaccg
     7081 acggcacagc gtggcacgaa cgcgacggaa tccctcacgc caacggggga ggcgtgaggg
     7141 atttccggct tccctggtca gcagacgcgt ccagcagaca cgtccaacag gcacgtccag
     7201 cagaaacacc cagcagacag ttcagcagac acgttcagaa gagaacggag gaccttagtg
     7261 tccagcgctc acaccgtctt ccccgtcccc gaagaggtcg gacgccttta cgaccgcctg
     7321 accgcgctcg acacgggcac ggtcggcgga agcctccacc tcggctactg ggacgccgag
     7381 gacgacggtg tgccgctcgt ggaggccgcc gaccggctca ccgacatgat gacggaccgc
     7441 ctgcacgtcg acgagagcca ccgggtcctc gacgtcggct gcggggtggg gcagccggcc
     7501 atgcggatcg cccggcgtac cggtgcgcac gtcaccggca tctccgtgag ccgcgcgcag
     7561 gtcgcccgcg ccaccgctct cgccggggcg gcggggctgg gcgaccgcgt ggagttccgc
     7621 tacgccgacg cgatgcggct ccccttcgag gacaactcct tcgacgccgc catcgccatc
     7681 gaatcgatct tccacatgcc cgaccgcgag caggtcttcc gcgagatccg gcgggtgctg
     7741 cgccccgggg gccggctggt gctgaccgac ttcttcgaac gggctccgat cccggccggg
     7801 ctgcgctccg ccatggaccg gtgcctgcgg gacttcatca tgaccctggc gcggcccgag
     7861 gactacgtgc ccatgctgag ccgggcgggc ctgtgcttca ccgaactcct cgacatcacc
     7921 gagcagtgcg tgcgccggac gttcgagcag atgagcaggt cccgccgggg ccagggagag
     7981 ctgcagaccg tgttcgacga ggcggcgggg gagaagttcg gccctgccga catgatcgac
     8041 ttcgacgagt tcggctccgt cctgctggcc gctcagaagc cggcctgaca tgacatgacc
     8101 tgacatggcc gaagaccgcc ccgagcccgc ctttgcgccg gccggggcgg tcggggcggc
     8161 cgggacttcc ctgccggaac tccctgctgc agcgacctga ctggtcgtca taccgcgggc
     8221 gcgcgactcg accgcacact atcggccacc tcgcccccgg cgtcagggct ctccgaggtg
     8281 ccggcccgag gcgaggaata tgcggataat cgcaccaggt cagccctggt cgccgccctg
     8341 aagcaccccg gaaggccggt gcgggtcaca cgaatcatcg ccccctcgtg gcgcaaagac
     8401 gacggtgccg ccccggccac ctcgaccaac atcatcaggc gctgcttcac ttgcgcggcg
     8461 ggagccccca gtcgcccgat cgggctactt caggggctcc ctcttgacgc atgcgccgag
     8521 acggaagagg gaccgggatg tcgaccgcag cgcactggca acagccggag cagcccgaac
     8581 agcaagcagg ccgggcccag ctcagcctgg ggatcgccgc tgcgcggaat ctggcgagca
     8641 ccaccaagac ggtcccgcag atgcagggca tcagctcgcg gtggctgctg cgcatgctgc
     8701 cgtgggtgga ggtgaagggc ggcacgtacc gggtcaaccg gcgcctggtg tacgaggtcg
     8761 gcgacggccg ggtcaccttc gtccaggaag gcgccgcggt caaggtcgtc cccgccgagc
     8821 tgggggagct gccgctgctg cgcggattcc acgacgacga ggcgctgcgg gcgctggcag
     8881 agcgctgccg gcagcaggag tacgaacccg gccaggtgat cgccgaacgg ggccgtcccg
     8941 tcgaccacgt ctacctgatc gcgcgcggca aggtcgagaa ggccggcacc gggaagtacg
     9001 gcgaggagac ccggcgcggc gtgctggccg acggcgggtt cttcggcgcg cagacggtgg
     9061 ccgaggagcc gggcaagtgg gagttcacgg cgcgcgcgat gacggcgtgc accgtcctgg
     9121 ccctgccccg gtacgcctac gaggaggtgg cgggccgcca cgaggagctg cgggcccacg
     9181 tccgccggcg ccaggccgag aaggcgcgtc cgcagaacaa gaagggcgag gcgaacataa
     9241 ccctcgcctc aggccacacc ggcgagccgg ccctgcccgg caccttcgtc gactacgagc
     9301 tcgccccccg cgagtacgag ctgagcgtcg cccagacgat cctccgggtc cacacccgcg
     9361 tcgccgacct ctacaacgac ccgatggacc aggtcgagca ccagctgcgg ctgaccatcg
     9421 aggcgctgcg cgagcgccag gagtccgagc tcgtcaacaa ccccgagttc ggcctgctgc
     9481 acaacgccga ctacagccag cgcatctcca cccactccgg cccgcccacg cccgacgaca
     9541 tggacgagct gctgagcatg cggcgcggca ccaaggcttt cttcgcccac ccccgggcca
     9601 tctccgcgct cgcccgcgag tgcaacaagc ggggcctgtc cttcgacagc gccgacgtga
     9661 acggccaccc cgtgcccgcc tggcgcggcg tgccgatcct cccctgcggc aagatccccg
     9721 tctcggaaga gggcacctcc tccatcctcg ccatgcgcct gggcgagggc gagcagggcg
     9781 tggtcggcct gcggcagacc gggatcccgg acgagtacga gcccggcctg aacgtgcggt
     9841 tcatgggcat caacgaccag gccctcatct cctacctggt cagcacctac tactcggcgg
     9901 ccgtcctcgt ccccgacgcg ctcggcgtcc tcgagaacgt cgaggtcttc cacaccccgt
     9961 cctgacgggc accccacccc ccggaacgga gagaagaacc ttgtcactgc tgtcccggat
    10021 caccgcgccc ccggcacgtc acgacgtggc gcggctcgtg gccacgctgc tcacccaccg
    10081 ccaggacgcc cgccggtcgg caccgcaggc gctgcccggc accacccctc gcccggcggg
    10141 caccgccctg cccaccggtc cgacggggct gggcacctcc gccgcacgcc tcgcggccgg
    10201 gaaggccagg gagacctggc gggccgggaa ggccggggcg gccggggaag ccgggaacgt
    10261 cgccgggaag ccggcggccg gcgccccggg tgcccccggc atctccgttg ctcccgttgc
    10321 ttccggcgcc gcctcggacg accgtggcac cggcgtcccc gagctgtact gcccgcccgc
    10381 gctccgcgac gacccggcgc tcgcccgtga ggtcaacgac cggctggtgg actgggcgga
    10441 cgaggccggc atctacgccg gacggctcga ccgcgtccgc gcggccgact tcgggcgcct
    10501 gatgatgctg gcccaccccg acagcgacga cccggaccgg ctgctggcgg cgggcaagtg
    10561 cgccctggcc gagtgggcca cggacgacta ctactgcgac gacgagacca tgggctccag
    10621 gaccgaactc ctcggctccc acctcggact cgcctacacg gccctcgacc cctcccacct
    10681 tccggcccgc tacgcgccgg cgtgggagga ggagatgcgc gaggaccccg tacgcgtcgc
    10741 cctgcgctcg ggcctggacc acttgtcccg ctacgcggag ccctcgcagg tggcccggct
    10801 gcggcacgag gtcgccgtcc tcttcgtggg ctacgagcag gagggctcgt ggcgctccca
    10861 gggccgcatg cccggcgtcc gcgagtacct ggcccaccgt cagatcaaca gcttcctccc
    10921 ctgcatcgca cttgccgacg tggtcggcgg ctatgcgctg cccgcctccg aatacgccga
    10981 accgcgcgtg cgccgcgccg tgacgatggc cgccacggcc gccaccctcg tcaacgatct
    11041 gtactcgatg acgaaggaac acgactcctc gggcgtggag ttcaacctcc ccgtcgtgat
    11101 cgccgaggag gacggctgct cgctgcgcga ggccatcgag cggagcgcgg agatccacga
    11161 cgagctcgtg cacacctacg agacggaggc cgcggccctc gccctcaccg gaacgcccga
    11221 gctgcgccgg ttcctcgcgg gagtctgggc ctggctcggc ggcaaccgcg aatggcacgc
    11281 cggaagcccc cgctacaacg gcgcgtgagc ccgcccctct ccactgccgc ctcctccccg
    11341 acatcccgcg cacatcccct gtacgtcccc agcacaccca cgtgacacca cgcgtgccgc
    11401 gcattccacg cgccatcacg cacgcatgcc gcaatccgta aggagcaccc catgacgacc
    11461 acgcccgccc ccgccgcgtc cgccggcgtc ctgcgcaccg cctaccagaa gtccgtagcg
    11521 gagtactgga acaacgagaa ggacccggtc aacctgcgcc tgggcgacgt cgacggcctc
    11581 taccaccacc actacgggct cggcgactac gatccctccg tcctggaagg ccccgaggag
    11641 acccgggacc agcgcatcat cgaggaactg caccggctgg agtcggcaca ggccgacgtc
    11701 ctcctcgacc acctcggccc catcgccccc gaccagcgcg tcctggacgc cggctgcggc
    11761 cgcggcggca ccagcatcat ggccaacgcc cgcttcggct gccgcgccga cggcgtctcc
    11821 atctcccagg cccaggtcga cttcgccaac gagcaggccc gcaagcgcgg cgtggacgac
    11881 aaggtccgct accacttcgc caacatgctg gacaccggcc tgcccaccgg cgacttccag
    11941 ggcatctgga acaacgagtc caccatgtac gtggacctga acgaactctt cgccgagcac
    12001 gcccgccagc tggcctacgg cggccgctac gtcaccatca ccggctgctc caacgacgtg
    12061 accggcctgc gctccaaggc cgtcagccgc atcgacgagc actacacgtg caacatccac
    12121 ccccgcagtg cctacttcaa ggccatggcc gccaacggcc tcgtgcccat caacgtcgtc
    12181 gacctcacgg ccgcgaccat cccctactgg gagctgcgcg cccagtcctc cctcgccacc
    12241 ggcgtcgagg accccttcct gaccgcgtac aaggagggca gcttccacta cctcctcatc
    12301 gccgccgacc gcatctgacg ccccggcctg tcccggccct gtcccggccc ccgccgcggc
    12361 ctcgaggagc gaggccgcgg cgggctgagc cgagcggctc agtgctccgg agtcgtcgcg
    12421 tgtgcccgct cgaagagcct ctcggcctcg gtgatcgccc gggtcagggc ctcgggctcc
    12481 ggtcggagcc cggccacgat cgcgtcgatg ccccgcagac cggcctgctg cagaagccgc
    12541 ttctcgttgg tgacccattc accgcgcccc gccagcaccg cgtgccccgt ctgcatggcg
    12601 gcgacggcca gcgcgcccgc gacctcggcg gcctggccgc gccgcacgta cgcgcccttg
    12661 gcgtagcgga gcgtcagggc cgcccgctcc cgccacatcg gcggggccgc gtcgcgcagg
    12721 gcctgcgggt actcggggcg gggcaaggtg ccgcgcagca cctggttgac ggcgagttcg
    12781 gcgaccacga ggtagctggg gatgcccgca aggtggaaca tcagcggttc ccagtggaag
    12841 cggccctgcc gggcctcggc gagctcgtgc tcgacgacgt cgaggtcgcg gtagtgcaca
    12901 tcgacgtgcc gcccgtcgat cgtgagccac gccccaccgt tgaacacgcc accgccccag
    12961 cccccgatct cggagacctc gccttcccag ccgacggcgc gcagctcggc cgggtcgaaa
    13021 ccgccgcggt agtacacggc caggtcccag tcgctgtcgg gggtgtgggt gctttgagcc
    13081 cgcgagccgc cgagggtgac ggcgagtacg gagggcaggg ctgcgagccg gtccgcgatg
    13141 ggggcgagga agaggttgtc gtcgtcggtc atggggatcc gatcctgaga acacgcgaga
    13201 agtgaatgac aggcgatgcg ggcacaccgg acgcacgccg gggcagaggc tcggacggcg
    13261 tgaggtgatt cgtgtgccgg acgtgtcatc acttcatgac gcagactgta cctgccggca
    13321 ccgacgaggt gcgcggtgca ctcggtccgg gctggtgcaa ctctgcctgt tcgcccagtg
    13381 cgcgttgcgc tggggcacct tgccccctca tcggcgagcc tgtatgaagg gagggttgcg
    13441 ctttctgtag gcaaagagag gcgtaccctt gcggaaacaa gagaacgtcg gattgccgga
    13501 agtacagtac ggccggggct caacccacaa gactgctgca cgcgagagcg ggccactcca
    13561 aaggctcatt ggcttgcaga gtgccgcggg taacgcggca attgttcaga tgctccgtca
    13621 ggccgaccgt ccccggaccg gggaacagca ccagcaagcc gcgcagcctg aatcgcagag
    13681 ctctgctgtc catgaggtgc tgcgcagtag gggtcgcccg ctggccgatt ccatccgcag
    13741 tgacatggag aaccgcctcg gagctgattt ctccgacgtg cgcattcacg acgacagcgc
    13801 cgccaaagcc tcggccgctg aggtcggcgc ccgtgcatac acctccggca gccacgtagt
    13861 catcggcgac ggtggtgggg acaagcgcac cctggctcac gaactcaccc atgtaattca
    13921 acagcgtcaa ggccccgtcg ccggcgcgga caacggatcg gggctgaagg tctctgatcc
    13981 ttccgaccgg ttcgaacgcg aagcggaagc caatgccgca cgagcgatgg tcggttcggc
    14041 tccgccccag gaagtccagc gtgtgactcg atcgtactct tcgtcgaatg gtgacacacc
    14101 gctccaacgg atggccaagg ggaacacgaa aggggcgagc ggcggaaagc aaggaggtga
    14161 aaagcaggga ggtggaaaga agggcaagtc ggccggcgcg gtggccgaga gttccggcgg
    14221 tccacaggaa gacattgagc tgatttccag gaccgtcggt gagcacgatg tgcgagtcgt
    14281 catggacagt caccagaaca agcaccagtc gccgcctatt ggggaactca aggcgtacac
    14341 ggggggccgg ggcaccaagt ttaaatcgca tgtggacctg aactggcaca gggccaactc
    14401 ggcagaatat cttgtgcgct gcatccccgc actctttggc gaggcggata ccgaagagct
    14461 tgcatggtcc ccgatggggc cggggaagtt cgcagcaaag aaggtggccg tggacgtcaa
    14521 cggacaggct gtcatctttg acgccatcgc gactgtcaag ggtaacgcag tcactattaa
    14581 ctaccactgc aacccgccat catagggtgc attggtgaaa tcttcaggtg acgccccggc
    14641 ataccggtcg gggcgtcacc tgtccctggc cctgggtgcg tcggctgttc aatgtcgtcc
    14701 gccgcctcgg ccgcacctgg gcagtccgga gagggtcgat gtccaacgtt cagtcagctg
    14761 tgcgggcgga catggaggtc gagtccgctg gtgcccggcg tctccacgcc cggcaggagg
    14821 cgcatgtcgt ggtcgtagtc gccgccggtg agcgtccggt aggggacggg ggcgtcagtg
    14881 aagaggccgt cgaggcggtg cgcgtactcc cgcttcgccg cctcgaaccg ctcctccggc
    14941 aggtcgttgg cgcggtacac cccgaacggt tcgagcggcg tcatgcccgt gaaccagaac
    15001 aggccgtgct gcaccgggtg gaggacgtcc gcgaggcgcc cgtgaatgcc ccggtcggag
    15061 aacgacgatt cccgggcgcc cgccgtcacc gacaccagcg cccgccgccc tccgagggcg
    15121 ctctcggagt agggcggagg gagggtcggg ccatagccga agccggcggt gaacacccgg
    15181 tcgacccagc ccttgagtat cgcgggcatg gagaaccacc acagcgggaa ctgcaggatc
    15241 accgcgtccg accagcgcac cttctcctgc tcggccgcga tgtcggcgga cagtcggccc
    15301 gccagcgtgg cccgctcgga ggcggccatt acctgcagcc gccggtcccc gggctcgtcg
    15361 gggaagtcgt cggcgtcgac cgcggccttc cacttcatcg cgtagaggtc ggagacctgc
    15421 acctcgtggc ctgcggcgcg caggtgctcg gtcgcgaagg cggccaggga ggcgttgagc
    15481 gagcggggct cggggtgggc ggtgacgagg aggatcttct tttgctgcgg ctgctgctgc
    15541 ggtgcggtgt cgttcatgca gctacgttct cccgggcgcg cccgggtagc cagcaccctg
    15601 ttttacctgc ggatccgtga tcctggtact gccagggcca cgcatactgg agcgtatgaa
    15661 cggcgtggac gacagtcgca tcggaggagt cgacggggtc gacggggtcg gcgatgccca
    15721 gcgggcgctc ggcaggttcc tgcgtgcccg cagggggcgt gtcgcaccgg aacaggtcgg
    15781 gctcgcaggc ggtcgtcgcc ggcgggtgcg cgggctgcgc cgcgaggagc tggcccagct
    15841 cgccggcatc agcgtggact actacgtccg cctggaacag ggccgggcca cccagccgtc
    15901 cgcggaggtg ctcgacgcgc tcgcgggggc gctgggcctg gacgcggcgg agcgcaagca
    15961 cttggacacg ctcgccggcg ccaggcgcga ccctgtgccc agggcggagg tcagccccct
    16021 cctgcagcgg gtgctggatt ccctggaccg cttcccggtg ttcgtgacgg accaccgtat
    16081 ggacgtggtg gcctggaacg ccctgggcgc cgagctgatc ggcggcgcgc ccgggccggg
    16141 gagccgcgac cggaacaacg ccaggtacgt cttcctcgac ccggcctccc gggccgtcca
    16201 ccccgattgg gaggccaggg cggcggaggc cgtgggtcag ctgcgggtcg ccgcggggcg
    16261 ctatcccgac gatgcggagc tgtccgccct catcaacgaa ctgtccgcgc acagcgccga
    16321 cttccggagg atctgggcca ccggcgaggt cgtgatgtgc gcagcgggcc gcaagcggct
    16381 gcgccacccc gaggccgggc tgctcaccct ggacttcgag acgctgcacg tgccggcggc
    16441 gccaggtgag acggggctgg tggtgcacgt gttcagcgcc gaggagggga gcgaggaggc
    16501 gtccgccctg gcccggctgg cgacggccgt gacgggtgtg cccgttccgg cctgacgagg
    16561 gtcttcgggg ccccggggcc ctggagaggt tgagggctag cgcgtctttt gggggcgcaa
    16621 ccgcacacgc gcagtggtgg gcagcgacgt cgacgcggag cccgggccga gcgggcggaa
    16681 gccgagaccg tccgggatct cgtactccca gtccaaggag cgggcggcgc ggacgagttc
    16741 gcccgcgccg ccgaccttca ggaggacctt ggtccggcgc ttccacgcca cgtcgacctt
    16801 gtaccggtgg ttccagaagt ccgcggacgt gccgaggtgc aggacgagtt ccgtcagttc
    16861 gggggacagc cccgtcagcc ggtcctgcat cctccggctc cagtcgttgc cggggtcgag
    16921 caggtcatcc acgtgatcgg tcatgcgccg atcttgccaa ggcccactga cagcgccccc
    16981 ggtacggccg tggtcagcgt gtgcggtggg tgccggtgct ggtgtcggtc cgggcggggg
    17041 cggggccgga gacggggccg tcgaaggtgt gtgtggtcgt gctcccggcg cgtacggcga
    17101 tgccgtcggc ggtggcgcgg ttcgacggcg tgccgggcgc cgtgggggac cagtaggtga
    17161 tgtcccaggt gccgcgctcg ggcgtcacga agtaggtggc ggtgccgggg accgtcagac
    17221 gccggctgag accgacggtc ggtccggcgc tcggctgcag gtcgaccgtc gcgggcccgg
    17281 tggtgcccgg tcgggcaccc ccgcggacct gcacggtagc caggtccttc gcggagggat
    17341 gccgggtgag tccggtcagt gcgcgggacc cggtgaagac gtagccgagc gcgtagtcgg
    17401 cggacttggt cgtccagtat gcgtcgtacc actgcttcac cgacccgggg ccggcgtcgg
    17461 ggcccaggtg cgcgacgcgc aggttcggcg tggagaccat ccggttgacg agacgggtcg
    17521 cgccgccgtg cttgacctcg gtgaccatca tgccgtgcag gaacgccgcg gaacggtcgg
    17581 gcggccgtac gtcgacgggg gccgtggtgc gcgcgtcgac ggtgacggtg gtgtcgcggt
    17641 cgagatcgag acgcggctgg acgatccagt ccgcgccgtc cgtgccgtct gcgccacccg
    17701 taccggccgc ggccttgccg gtggtcaggt aactgtccag taggtagcgg cccttgggca
    17761 ggcgcacggt ggtcgtgccg gaagtgtcgt gggggttgac ggactcgccg gagcccgggc
    17821 ccgagatgcc ggtgacggtg gtgtcgtagg cccctgtcgg gctcccgacg cggtcgaggt
    17881 gacggatcgt gagacggtac gtctccggct gctgcgctgc ggcgaaggcc tggccgtccc
    17941 aggtcgacgt tgcacagccg accagggtga cagcgagcgc gagagcccgg aggacgctcc
    18001 ggcggggtac ggaagaagtg ttcatgatct gcactgcgcc gcagggcgcc ggagttcctg
    18061 tggtctgcgt cacccggggc ggggagccgc ggtgcgggcg gcagctcctg tggtgcacgt
    18121 caccggaacc ggcgtgccgg acgtggcaga gcagaacgtg gaatcactga tgaggaaccg
    18181 cttccgccgc ggagaccgcg acgccttggc ggagctgtac gacgagcacg cccaagtgct
    18241 gtaccgctac gccctgcggg tgacgggcga ctgggcggag gccgaggacg tcgtctcggc
    18301 gaccttcctg gaggcgtggc gcggccggga gcgcgtgcac cccgacgggg acagcctgcg
    18361 cccctggttg ctgggaatcg ccaccaacat catgcgccgc aacgcccgcg cacgccgcct
    18421 ccgcgacatc gcgctcgccc gcgtcccgga acgcggctcg attcccgact tcgccgacga
    18481 cctcgtcacg cacctcgcgg acaccgagca actgcgcgcc gcgcacgccg cactcgcccg
    18541 cctgcggcgc cgcgaccggg aggtcttcac cctcgtggtg tgggcgggcc tggactacgc
    18601 ggcggcggcc gaggcgctcg gcgtgccggt cggcaccgtc cggtcgcgtc tgtcccgcgc
    18661 ccgcacccgg ctgcgcgagc tcgccgacgc cgaagcacgt gcggtgcgct cgaggggctc
    18721 gcacacctcg aaggcttcgc atatctcgga tacctcggag attcggcggg ccgaagccgg
    18781 gcggccgcgc gcggcggtcg gcgacgaagc cgccgccgct gccttcgacg agccgctcgc
    18841 caagccgctg gacgcgccac ttgccgcgcc gctcggcccg ccgcgcggca agtccctcgg
    18901 caagcctgtc atccggtccc ttgccgagcg gctcgacagg ccccacgccg agccgctcgc
    18961 caaggccccc gccccaccgc tcgaccagtc cgcaccgggc cgcatccccg cgccccggtc
    19021 ttcgacccag gagaaccacg gatgaacacc accccgcggc gccgtccgct cccagggtcc
    19081 gaggacaccg aacgtctcgg cctcgccgac ctgctgcccc cgccgccggt ccccggcctg
    19141 gaccccgacc gtgaactgct gctgaaggag gcactgctgc gcgaggccgc cgccgacgca
    19201 ccgcgtggga gcctcgccgc ccgggcctcg cgcctgcgcc cgtcccggcg cctggtcgtc
    19261 cgggtcatgg ccccggcggt cgcctgcgcg ctggcggtcg gcggcgtcgt ggcggtgaac
    19321 cttgcggaca cgcccgggcg cggagtgccc acagtcccgg aacgtgacgg tgccgaccgc
    19381 acgcccgaag cgacccgtct cctcgaccgc atcgccctcg ccgccgcagc ggcaccgcag
    19441 ccgacggtcc gtgccggcca gttcgtatac gtcgagagca aggtcgccta cgcctcgcag
    19501 agcgccgcgg gaggtgccgt gaccatggct cccgtgcaca cacggcaggt gtggctgtcc
    19561 gccgacggca gccgcccggg cctgctgcgc gaggagggca ggcccgacgc gccgctcggc
    19621 gccgacgaca ctcccgtata cgccctcgac ggcccggggg ccacgccccg gccgaccacc
    19681 ctgcacaagg gcccgtcgat cagcgacccg acccaccaat acgtcgcgtc gctgcccacc
    19741 gaccccgacg ccctgctgaa gctgatccgg gacgagacgc gcgggggaag ccaggaccgg
    19801 gaccgggacc gggactcgga cccggaccag cgggcgttcg ccgcgatcgg cgacctgctc
    19861 gccgagacct gggcaccccc gaaggtcagt gccgcgctct acaaggccgc cgcgaagatc
    19921 cccggggtca cagtggtgcg gtcggccgcc gacgccgcgg aacgggcggg catcgccgtc
    19981 gcccgcacgg cgcacgggca gcagacgcag tgggtcttcg accggagcac gtacgcgttc
    20041 ctcggtgaac ggacggtcct caccgaagcc ggcgatgcgg gcccggccgg gacggtcgtc
    20101 ggcgcttccg cggtcctcgc aaaagctgcg gtcgatcgtg tcggggtgat tcctgcgtcg
    20161 agcatgaaat gaagtcgtga ccgatggggg tcgtttcacg tcattgggtg gaatttttgg
    20221 gcatgaaagc gcccttcagg gaaatccagc cacatcgcac cgccgctcgc cgaccgggtg
    20281 ggagggctct tgacagcgac ccgattgcat gaaggggcgt tgctgtatgc ggaatgcggc
    20341 atgtctgatg actccatttg caggtggaac gtgcgacggg cccatggacc gccatcgcgc
    20401 tgtccggaag ccgcaagtgc tgtgcactgc tggccagttc gctcggatgc ggggggagtg
    20461 ctgacggcat aatctcgacg cggtctgagt cacccccgcg ggggtcatcg gaaagcggct
    20521 gcctcttcgt cacacctgca acaggtgtgc tctgtcctgt gcgccgtcga tgggctcacg
    20581 catcaacccg agaaccggac ggagcgcgct gtgacagacg gcatcatcga gtacccgctc
    20641 ggaaccgcat atacaccgca ctcggtgggc gtcggcaatg ccggcaaact ctggatcacc
    20701 acgagcaaca agtgcgtcat tcgtttcgat ccgcaggccc caggtaggct gagtcacgcc
    20761 aatcacgcac tcatgcccga gctctccgcg gggatcatga aggatccgct cggccacgcc
    20821 aaggtgtggt tcgccgcacc ggcccctccg gcgaaggagg acccgatcgt ggcgatgcag
    20881 accacggcgg gctaccctct cacgttcgtc ccgctggagt ccgacgccaa ggcggagagc
    20941 atcgagctcg agacgacgag ggcgggcggc gacggcacgc cgacgtacga gtacagcctg
    21001 ctcttcgccg aaccccagta ccactacgtg ggtgtcctgc cgatcacacc gtccggcccc
    21061 ggccaggtcg agaagatgac cgtcgagcac agcacgtggc tctgggacgt ggccgtcagc
    21121 accgacaccg acggacagac ccacacgtac tgggtcaccg gccaggcgaa cagcagcgcg
    21181 tccccgcccc gcacccagaa cggcctgtac aagctcgctc ctgggcagac cacctgggaa
    21241 cgcatcgccc ttcccggggg gccggggcag aagccctact acgtgaaggt cgacacggag
    21301 gcggtctg
//
