LOCUS       NZ_LNBE01000001        21029 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_1, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000001
VERSION     NZ_LNBE01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 441060)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 441060)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 441060)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 235956
            Orig. end                         :: 256985
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21029
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="5"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21029
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21029
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11029](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11029)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            352..825
                     /locus_tag="AS857_RS00975"
     CDS             352..825
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1298: putative carboxymuconolactone decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010309000.1"
                     /locus_tag="AS857_RS00975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_058041155.1"
                     /transl_table=11
                     /translation="MDARLNFLTDPATGKAIKHFMAVGKILKDSPLPTATQELVSLRVS
                     QINGCAVCIDMHTKEAAAASESSVRLNLVAAWREATVFTEAERAALELAEEGTRVADAA
                     GGVSDEVWAHAAKHYDEEQLNALVILISFMNMANRFNVITRQPAGDYKAGQFH"
     gene            984..1907
                     /locus_tag="AS857_RS00980"
     CDS             984..1907
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059031.1"
                     /locus_tag="AS857_RS00980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="WP_058041156.1"
                     /transl_table=11
                     /translation="MSKVEEFEELRPLLFSIAYRILGSVSEAEDAVQETWLRFDGSSTR
                     PTSTKAFLSAAVTRISIDVLRSARVRREEYVGPWFPEPLLSDPYQDPARSVELADSVSM
                     AALLLLERLSPLERAVFLLREVFAFGFDEVAVAVGRSEAACRQLLVRARRHMEDGRPRF
                     AADREERQELATRFFDALKNGDVGGLQNLLAADVQMVGDGGGKAPQLARAIVGAENVAR
                     LLGTVYPWLVRIDVSLEPHEVNGQPGAIFRDRDGKVLHTMVLDVLDGQIQTIRSVINPD
                     KLGHIGPVADAWAVDREVKQVRRQSN"
     gene            complement(2092..4281)
                     /gene="katG"
                     /locus_tag="AS857_RS00985"
     CDS             complement(2092..4281)
                     /EC_number="1.11.1.21"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]"
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="katG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237976.1"
                     /locus_tag="AS857_RS00985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="catalase/peroxidase HPI"
                     /protein_id="WP_058041157.1"
                     /transl_table=11
                     /translation="MSGSESENPVIPSPTPTPSHPRTNRDWWPNQLDLQVLHQHSPRSN
                     PLGAEFSYAEEFATLNVDELKQDVFEVMTTSQDWWPADYGHYGPLFIRMSWHAAGTYRI
                     ADGRGGGGSGAQRFAPLNSWPDNASLDKARRLLWPVKQKYGRKISWADLLIFAGNCAIE
                     SMGLKTFGFGFGREDIWEPEEIFWGPEDTWLGDERYRGDRELTGPFGAVQMGLIYVNPE
                     GPNGNPDPIAAARDIRETFGRMAMNDEETVALIVGGHTFGKCHGAVDPSYIGPEPEAGP
                     IEQQGLGWRNTCGSGKGADALTSGLEGAWTSEPTTWDNGYLDNLFRYDWELTTSPAGAN
                     QWTPTDPSAKDTVPDAHDPSKRHAPMMLTTDLALKLDPVYAPIAKSFHENPDKLADAFA
                     KAWYKLLHRDMGPLPRYLGPWIPEPQLWQDPVPDVDHELVADEDIADLKRRILASGLSV
                     PQLVTTAWASAASFRGTDKRGGANGARIRLAPQKDWELNDLPEVTEALQTLEQLRQDFN
                     RSQAGGKKVSLADLIVLGGCAAVERAVKNAGHDLTVPFAPGRTDASQEQTDVESFSVLE
                     PKADGFRNYLRAGEKLSPETLLLDRANLLNLTAPEMTVLIGGMRALNTGYKQSPHGAFT
                     HRPETLTNDFFVNLLDMGTEWKASTSAENVFEGRDRATGEVKWTATAVDLIFGSHSQLR
                     AVSEVYASEDAGEKFARDFVAAWDKVMNLDRFDLA"
     gene            complement(4483..8178)
                     /locus_tag="AS857_RS00990"
     CDS             complement(4483..8178)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151961.1"
                     /locus_tag="AS857_RS00990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058041158.1"
                     /transl_table=11
                     /translation="MTIEELRALIGRHTQGTTLTLPAADLGTGPAAGLIGTWLDGTLAI
                     TDLHREDDNDSVVVHGTLTVDGLGLTGMLVRGITFGLDPVDQDPTLYVPLALLTDWTFA
                     TSFPATAGSDLAALAFPAPPALLLTSVGRPPRDGYPALDPGLTFHAPQVTDPEQLGALA
                     ALLRPRPGTLSLTGPVVRRPVNEQGGEVRTDIALRSTPQPDGAFSASFYLWAGSRSDGG
                     NGSPVTYGLRLAADLVMGKAVGATISAPLPSGGGVLALTADRFPERLTPVDLAAWNGTG
                     AAVHQLVDQEGFALGDSVRLTEITATIDPAAFGKGGLAAALTQVKATVETTPGTTWPIM
                     GDDLALTGVGATLTVTNPLQPSRTAEVTGRGDFTVAGDFSLHTTAKIPPGTFELALDES
                     TPAELRGVLHHFLPRADLTGVPELTLKKFSGTATPKKGTYEVEASVSSDWHIDIGAARI
                     SLTEAGLKLAREGGEGAGGSTVQQLEGTADAPNGGSRTTGTLSANAELGPAQGGNGDDS
                     VKFSAAWTIPGAFELDGNFPDVDLTALLKSLACQADVALPDGLPRVSLLQPAVTVRLGN
                     ALSGQPASGKSYELTVSTTATFAEAQLTFFGKAAKKAEGGTLFAAAFWQKDWVWSPGKV
                     SDWAPVLGFLDGITFAKSGLAVSSADGVTVDAGASPPETLPSTLGKGLTFFTELGLTGP
                     LAPLKELFQDASGIHLTARLTTPVQESEFTASIAEQKTRDGFGGLTLAFRPAQREVSLQ
                     TSWNFTVPAVGSAPATLLQFVAGGSLKGTEFHLFLVLKPAAGSGAEEAAGRPAPHQLAY
                     ALREPGHTLLTADPILGADGRLVHDATPHRTGGTAYSLVRDTASRLAGLSAPPSGDDRP
                     TWKNAFGVEGFDVHYFYMQIGYGAETGLTLGAGGSVIIGEATLDLDIEGGFNPEPFVTV
                     FHFDLGTVHKDTGISVWEMLAVLVAPPDWLSFLQQIVLHKLAICVVIDPNGWTNRETGE
                     EWKQGFYAKGDIDFFGNNWRFEVNVSDEGLYAYSAIAQPMKLGNVLTFSDASKNKGPQY
                     LLDTRGIRTGKLPPVIFSLSGNLDLLGLSASIEAQLGNEGFAFTIAADLLDILHGDMSC
                     SLSSTGLHASANVKLGFDVKIPRGVVLGGIPIPAGNLVGIHAEGGFDLTVDGNGVNLHL
                     TASCDAYLLDVHLVQFHFDQQFTIDKWDGIVSWLKDNPEKLFESLGEGIWDALKNCATT
                     SAAQLT"
     misc_feature    complement(7486..7488)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8185..9936)
                     /locus_tag="AS857_RS00995"
     CDS             complement(8185..9936)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS00995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_245699524.1"
                     /transl_table=11
                     /translation="MATKLLDFIYATMYKRINDPDSPYWLPTVLKDVRTPDGHTLNPLT
                     VDTWDIGQVDGDEGKTIGDTIGSAWWIFVGAAFDDAKDRGVPDLPDNLDPFNSQRDADH
                     PYPSLSLTTVTVDGLPNASVGELQNLTPTASGYRGTVRVTTAAYDTHGWQKQITVQGTY
                     SLVQHVVVIDDPGNGGGDGLKPPTKVVVKGLEGVEWPRQEIEGHGRFTVTAQDLAFDVT
                     LRAEATGTGADRVPQLTVESISVASQPEFHLDEKSLTIEGSTIDEATLQIWKQAAADAF
                     NSAEAGQALTAKLVATLADLSFRNQFSATVTTQLAKALDGVLGAVPTGTLPSDDTGFPA
                     KYGPLEVYLFDRLRASVNDTGSGFYPPTVVLGATDPRLEPYGLGDMDLGSYKIGVATAD
                     LGFKGGTIKGISNVLIPVEDAALTDGGIGATLRLGRLPGDAKVPPPPLTLTGTGVVSFP
                     DGTAAAAAPEHTSARDDDTIQGDITVTIDQPSATAALSFTGRDADELTIGLDALALAVP
                     TQNLQVTIHLKEPSPLEKIIQQVLNKDEVKQKIIKGVQDAVDAHRADIAKELTDNARAV
                     IHSKLEG"
     gene            complement(10001..11029)
                     /locus_tag="AS857_RS01000"
     CDS             complement(10001..11029)
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151964.1"
                     /locus_tag="AS857_RS01000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_058041159.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 5.7e-43, bitscore:
                     141.6, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLILDTTAPAEDVLGGVWTHLLADDLPRFEAFLDQALAPQQEYLT
                     DGERALYRHGKRLRPAVLLLAARMVHGPAPLPDKVLQGAVSLEMLHVATLIHDDIVDGA
                     ALRRGLPSVNAARGTETAVLVGDLQFVQAIRGFVRTIDRDSDMGLIELVLDTAFQIGCG
                     ELDELRTDLSQDPGLLAETYWRTADRKTAALFGLAAEAGVTLADGRSGDARRAGFYGRR
                     LGRALQVMDDLFDLAQDEAGSGKPRGMDLLRRRASLPLIYAMQEWGPEHAVSRIMRGEA
                     TGPGALERTLAEVRSGTGFSRAYTDARAQALEAVELLRPFAAGRYRYALEDLALHVVDR
                     GV"
     gene            complement(11033..12463)
                     /locus_tag="AS857_RS01005"
     CDS             complement(11033..12463)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151965.1"
                     /locus_tag="AS857_RS01005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058041160.1"
                     /transl_table=11
                     /translation="MSTPQPPQSGDRRTGPEAETLHLADTPGYPLARSVEMPGALDYAT
                     GSLSFRLGALQLRAEGQPEALAEEARGGIRRITHRIPAVVLTGRYALDAGPDEIHEIDT
                     AGNLRPLSAEARQPTLPAGARAVAPDHPDDTTIRKWTKRADSHRTKLMKTENGRQVLVQ
                     YGTHNESYYDIFNGNSSVSSNLRKKWAEKGVTQRMSQHTYDSTDPEAPVDARPSAGDQP
                     PVNDWHDPQEGVTYNGHAWQQRTTVQTALTTEAARIKRKDPAKAERLQAAATAAESFSK
                     SVQQTGNDGSHIILMTQDNVYTAIDRHSGEAPAVSPEEMARYNGLVAFADGEVQAPRAE
                     QEDRPEEPENWIALSDEDRAVIQGFGAAAYHDEAQQGPQATDTLHTGECTARLEDVRIT
                     TRLTPDGAPADVTVELPDLALSIDDEEWQGTAADVARERLSAMRFVHTLLQDAIAETVR
                     HAAFTGIAGYAHPQDGRS"
     gene            complement(12543..12914)
                     /locus_tag="AS857_RS01010"
     CDS             complement(12543..12914)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089074.1"
                     /locus_tag="AS857_RS01010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058041161.1"
                     /transl_table=11
                     /translation="MDALIDFEVVLDPAEPSVTFKATGLTDTALSATLEKIGLNSVSLY
                     PKSDAAKLVAAPANALALAAPAVLKGALEGKKSADIPLKTPLGTDITVQGQTVSVKLTS
                     PELGSHDGMLMVSGTFVVS"
     gene            complement(12962..13900)
                     /locus_tag="AS857_RS40825"
     CDS             complement(12962..13900)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS40825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_245699525.1"
                     /transl_table=11
                     /translation="MTETPYDLALGIHADALNSGLKSLHSLHRAVFQGAYGPETIEGIE
                     YTFSWDFLEPPELVLEKPDTELWNQMIKAQGITDLPDSGVLQIHAPQLQVNGTIAGDAL
                     PQGVSEVKVAVQAKVDTGAITIVPLGVRLSEIPDPYTRIMVEKVIVPNVLDQAKSLVSG
                     LKLPVQDLFGQQVSLTPALLDVTGTHLVLTATAGTGHAPAAGSPAGAATDWPSDKQVFL
                     LVGRDLMARLLQGALDQWQNTKLADDDKTVTGVASVWWKATFRKAENVKVDENDPTTIS
                     ADFDIPWSVGVDLFPVAPGKGCALFKASQES"
     misc_feature    complement(13979..13995)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.19"
                     /tool="antismash"
     misc_feature    13998..14010
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.22"
                     /tool="antismash"
     misc_feature    complement(14065..14081)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.92"
                     /tool="antismash"
     gene            complement(14227..15519)
                     /locus_tag="AS857_RS01020"
     CDS             complement(14227..15519)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS01020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_144440676.1"
                     /transl_table=11
                     /translation="MNCDLMVGVNADVVNSTSKEVYGQLYPQYFTGSQPFDVEGVTFTV
                     SWDVKEAPVFDLTPQAHSEAELRDRLTSGWAAGQVTEAELAEHVAALRASLDGSTFQVR
                     LNLVHVKVDSGDGNPVEEDIAVTVQVQATTSDKGLFSLNPLGATADAPSRVDKIFLDKV
                     VLPNVLEESRKVLSGITLPTPQIPGLSLSAPVPVLRPQRAVAALNLAEHGTPKPPFPDD
                     WPQSPFFALLGRDAVERIAQVATSYLEGKTFPAGDHKDFAGSSAYYKADISIHNVRCEV
                     VWDDVPTVRVHMAVSGSGSAGINWFWGGSTDAFYDLHLEPDPTVTLTLAMSGTTLKATA
                     TEVSSFDLKLVPTRGDIVSMIFSWVVDALSSTFGGIVGSALRGVEFPVGAFPAIPIDVE
                     PVHLDATPADLTLSRFGDALALQGDVTVTKR"
     misc_feature    complement(15319..15321)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(15829..17388)
                     /locus_tag="AS857_RS01025"
     CDS             complement(15829..17388)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979266.1"
                     /locus_tag="AS857_RS01025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="WP_058041163.1"
                     /transl_table=11
                     /translation="MSTSPKPIALPVTAVTCMEDRAHIERTAVLDLEAGVQRLRLGPVS
                     ALAVDRTLHAELTADHPTTVLDVRIVRTWTPREPLPSADDSALRRRVHLLEEEQLSLGQ
                     RRDRLHARLDLLGRLAADVLREIGEGAGSGESEGPRWARELDRVDDERDSYGEQLRAVE
                     ARLAAIASELREVWQAMGLSEEEPAELVGHVELTVEAAAAGPVGLRLSHLTACALWRPA
                     YRAVLDGGSLTLETDAMVWQRTGEDWSDVRLTLSTARSALATDPPWLGEDRLTLKDRSA
                     AERRTVDVELREEEIGDLGPVPVLGLPGVDDGGETRVLRSPAPVSVPADGRAHRVPLSV
                     FTTAASSEYACSPELSPLVTQVVRFDNRSGHALLAGPVDLVRGNGFSGRGTLDFTAPGA
                     SVELAFGSCDDHRVVREAEESRDSAGIMQRTVVTRTVRLYLSRFSAPAEQDERVVVLRE
                     RIPVSEVSAVEVRLRKEACSPAPAAVDAEGIARWDVALPPGGRRTVTLVYELSASSKVA
                     GL"
     gene            complement(17397..19490)
                     /locus_tag="AS857_RS01030"
     CDS             complement(17397..19490)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152332.1"
                     /locus_tag="AS857_RS01030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4139 domain-containing protein"
                     /protein_id="WP_058041164.1"
                     /transl_table=11
                     /translation="MTAETAQRWGSTLDSVVVYAQGAVCRRLARGSVPPDGRVRVTGLP
                     RSLDPGSLRARILGAPGVRVTEARVEVEAEPLGTGTPEGLQREVERLYDECAAAQGRRD
                     RQLSLIEEVRGLRPVPPARRREESHRRTPVDAWLGLADFVEERLTGLHARLVELEEALR
                     RAEHELGVATDRLDRASTDAPSAHVEATVSAVLTLDGTADAETGLDLELEYGVPGAVWV
                     PAYRLAYRQGDGTGDLVLRASVAQRTGEDWTGVRIALATADLRRRTDLPRLRSIRIGRR
                     QPAPAPSGWREPPAGLSDLFAGYEAAGPRPDTTGAVWAGSAPGGATGGSAPGPVPPPPA
                     AMAAAAPQGYGAPPPAPGGAGGHYPEDFEGEMPERSQPAPSQPGGMPHPGGSPFARAAA
                     PMAPAAPGRAAPPPPPPAPGPPQPSSTELDYAALVLCGPDEQGGRRGRLFPDAPFDAVA
                     AEYRRRAEAVAALPLPGHAVRPRESAGSFDHRFDATARADVPSDGTWHTITVAEIPVGL
                     RTEYLCVPSVEQTVYATSVLSNATGQALLAGPVEVTVDDEFLLTAALPTLAPGGVRRVG
                     LGPAEGIRVTRRTNLHESTSGLRNNTTVLDHRVHVELANRLARPVTVEVRERVPVTSEP
                     DVRIEERADWTAPEPGTGPEHHAPGTRVWRVELPAGDTAALDGRYEIRIPAGKALVGGN
                     RRS"
     gene            complement(19643..20170)
                     /locus_tag="AS857_RS01035"
     CDS             complement(19643..20170)
                     /GO_function="GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662194.1"
                     /locus_tag="AS857_RS01035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleoside-triphosphatase"
                     /protein_id="WP_063804128.1"
                     /transl_table=11
                     /translation="MPTRILLEGRPGVGKTTAVRRLASLLRTRTPVGFTTEEIREGTAR
                     VGFALETLDGRRAVLAHVAFPGPPRVGRYGVDLRVMDELALSALAPLSPGPAPGDLALI
                     DELGGMELASSAFRKAVGELLTADIDVVATVHARHDPFTDALKQRAGIELVHVTAQNRD
                     ALPGELAARLET"
ORIGIN
        1 gtgagcagca ggaccgatgc gggggacggc cggacaaggt gctttcgggc gagggtcatc
       61 gcagccatat ggccctattg ttgcccggcc cgggatcgct ggggtgacct caccgggcga
      121 aggcgccggt gtgagggaca caccttctga actgttcgac gaacgcacgg aaggagtcgg
      181 cccagagggt ggccgcgggg ccgggggcgc gtgaggcgtt cgcacagggt ggcggcgagt
      241 tcctcggccc cggccgggtg gggagaccca ggtcacatca gcccatgtca cagggggccg
      301 agctacctcg tctcaggggt gtaaccacga acgaccaccg aggagcacac catggacgcg
      361 cgactgaact tcctcaccga cccggccacc ggcaaggcca tcaagcactt catggcggtg
      421 ggcaagatcc tcaaggactc gccgctgccg accgcaacgc aggaactggt gtcgctgcgc
      481 gtgagccaga tcaacggctg cgccgtctgc atcgacatgc acaccaagga ggccgccgct
      541 gccagcgagt cctcggtgcg gctgaacctg gtggcggcgt ggcgggaggc gacggtcttc
      601 accgaggccg agcgtgccgc gctggagctg gcggaggagg ggacccgggt cgcggacgcg
      661 gccggcgggg tgagtgacga ggtgtgggcg cacgccgcca agcactacga cgaggagcag
      721 ctcaacgccc tggtcatcct gatctccttc atgaacatgg cgaaccggtt caatgtcatc
      781 acccggcagc cggccggcga ctacaaggcc gggcagttcc actgagagcg gcatcagcaa
      841 ccggctcggg gcccggctgc gggctccggg ccctcgggcc aggggcgtta ctctggcccg
      901 ccggtggtcc ggatgatcat cagaggacgc ctgcgccgaa gcacacggcc aggtgagagg
      961 gcttcagggg gagaggattc ggcgtgagca aggttgagga gttcgaggag ctgcggccgc
     1021 tgctgttttc gatcgcctat cggattctgg gcagcgtgag cgaggccgag gacgcggtgc
     1081 aggagacatg gctgcgcttc gacggctcgt cgacccggcc cacgtcgacc aaggcgtttc
     1141 tgtcggcggc ggtgacacgg atctcgatcg atgtgctgcg ttccgcgcgg gtgcggcggg
     1201 aggagtacgt cgggccgtgg tttcccgagc cgctgctgag cgatccgtat caggatccgg
     1261 cgcggtcggt ggagttggcc gactcggtgt cgatggcggc gctgctgttg ctggagcggc
     1321 tcagcccgct ggagcgggcg gtcttcctgc tgcgggaggt gttcgccttc gggttcgacg
     1381 aggtcgccgt ggcggtgggc cgttcggagg cggcatgccg gcagctgctg gtgcgggcgc
     1441 ggcggcacat ggaggacggg cggccgcgct tcgcagcgga ccgtgaggag cgccaggagc
     1501 tggcgacgcg gttcttcgac gcgctgaaga acggtgatgt gggcgggctg cagaatctgc
     1561 tggccgccga cgtgcagatg gtcggggacg gcggcggcaa ggccccgcag ctggccaggg
     1621 ccatcgtggg tgcggagaac gtggcccggt tgctgggcac ggtctacccc tggctggtcc
     1681 ggatcgacgt gtcgttggag ccgcacgagg tcaacggcca gcccggcgcg atcttccggg
     1741 accgggacgg caaggtgctc cacaccatgg tcctcgacgt gctcgacggg cagatccaga
     1801 cgatccgctc ggtgatcaac cccgacaagc tcggccacat cgggccggtc gccgacgcct
     1861 gggccgtcga ccgcgaggtg aagcaggtcc gccggcagtc gaactgaccc cgggagtggc
     1921 ggcgggcgtc cgggtgaacg gcgtgaaggc gatgccgtgg gcgctgaagg ggtgcgtacg
     1981 accaaaggtc ttgcatgcgg ccgggccccg caccgatacg tgcggggccc ggcggtggca
     2041 gccaaggcgc cagtcaaggc ggctgacggg acggctgacg gggcatcgcg ctcaggcgag
     2101 gtcgaaccgg tcgaggttca tcaccttgtc ccacgccgcc acgaaatcac gcgcgaactt
     2161 ctctcccgcg tcctcggacg cgtagacctc cgagacggcc cggagctggg aatgcgaacc
     2221 gaagatgagg tcgacggcgg tggccgtcca cttgacctcg cccgtggcac gatcccggcc
     2281 ctcgaatacg ttctcggccg aggtcgacgc cttccactcc gtgcccatgt cgagcaggtt
     2341 gacgaagaag tcattggtca acgtctccgg ccggtgggtg aaggcgccgt gcggagactg
     2401 cttgtagccg gtgttcaggg cccgcatgcc gccgatcaga accgtcatct cgggggcggt
     2461 caggttcagc aggtttgcgc ggtccagcag gagggtttcc ggcgacagtt tctctcccgc
     2521 ccggaggtag ttgcggaacc cgtccgcctt gggttcgagc acggagaacg actccacgtc
     2581 ggtctgctcc tgcgaggcgt ccgtgcgccc cggtgcgaac gggaccgtga ggtcgtgccc
     2641 ggcgttcttc acggcccgct cgacggccgc gcacccgccc aggacgatga ggtcggcgag
     2701 ggaaaccttc ttcccgccgg cctgtgagcg gttgaagtcc tggcggagct gctcgagggt
     2761 ctgcagcgcc tcggtcacct cgggcaggtc gttgagctcc cagtcctttt gcggcgcgag
     2821 tcggatgcgt gccccgttgg ccccgccgcg cttgtcggtg ccgcggaagc tcgccgccga
     2881 cgcccaggcg gtggtgacca gctgggggac ggacaggccc gaggcgagga tcctgcgctt
     2941 gaggtccgcg atgtcctcgt ccgcgaccag ctcgtgatcg acgtcgggga cggggtcctg
     3001 ccacagctgc ggctcgggaa tccacgggcc gaggtagcgc gggaggggcc ccatgtcgcg
     3061 gtgcagcagt ttgtaccacg ccttggcgaa cgcatccgcc agcttgtccg ggttctcgtg
     3121 gaagctcttc gcgatcggcg cgtagaccgg atccagcttc agcgcgaggt ccgtcgtcag
     3181 catcatggga gcgtgcctct tcgacgggtc gtgagcgtcg ggcacggtgt ccttggccga
     3241 gggatccgtg ggggtccact ggttcgcgcc ggccgggctc gtcgtcagct cccagtcgta
     3301 cctgaagagg ttgtccaggt acccgttgtc ccacgtcgtc ggctcggagg tccatgcgcc
     3361 ctcgagcccg ctggtgagtg cgtcggcgcc cttgccgctg ccgcaggtgt tcctccagcc
     3421 gaggccctgc tgctcgatgg ggccggcctc gggttccggg ccgatgtacg agggatcgac
     3481 ggcaccgtgg cacttgccga acgtgtggcc gccgacgatg agcgcaaccg tctcctcgtc
     3541 gttcatcgcc atgcgcccga acgtctcgcg gatgtcccgg gcggcggcga tcggatcggg
     3601 gtttccgttg ggcccctccg gattgacgta gatcagtccc atctgcacgg caccgaaagg
     3661 accggtcagt tccctgtcgc cgcggtagcg ctcgtctccg agccaggtgt cctcgggtcc
     3721 ccagaagatt tcctcgggtt cccagatgtc ctcccgcccg aagccgaatc cgaatgtctt
     3781 gaggcccatc gattcgatgg cgcagttacc ggcgaagatc agaagatcgg cccaggagat
     3841 tttccggccg tacttctgct tgaccggcca gagcaggcgg cgcgccttgt cgaggctcgc
     3901 attgtccggc cagctgttga gcggggcgaa gcgctgagcg ccggagccgc cgccgccccg
     3961 gccgtcggcg atgcggtacg ttcccgcggc gtgccagctc atccggatga agagcggccc
     4021 gtagtggccg tagtcggcag gccaccagtc ctgtgacgtc gtcatcacct cgaagacgtc
     4081 ctgcttcagc tcgtccacgt tcagagttgc gaactcttcc gcgtagctga attccgcacc
     4141 cagcggattg gaccgaggtg agtgctggtg gagaacctga aggtccagct ggttcggcca
     4201 ccagtcccgg ttcgtccggg gatgggacgg cgttggggtg ggggagggga ttaccgggtt
     4261 ctcgctctcg ctgccggaca cgtccgtcct tcttcctgtc tcggtgtttc cgccggaatc
     4321 cgatggtcgc gcaccgcagg ccgcaccgct gcgagaacac gcgagcaccc ccgagtgaca
     4381 agagtggcaa gcagggcggc cgtccgctcc gcccggcaca ggctcgtgcc gggcgggcag
     4441 gacgcggcgc gctccgcagg acgtgtcagg tgacaggacg tgtcaggtga gctgcgcggc
     4501 ggacgtcgtg gcgcagttct tgagtgcgtc ccagatgccc tcgccgagag actcgaagag
     4561 cttctccggg ttgtccttga gccaggagac gatgccgtcc cacttgtcga tcgtgaactg
     4621 ctggtcgaag tggaactgga ccaggtgcac gtcgagcaga taggcgtcgc agctcgccgt
     4681 caagtgcaga ttgacaccgt ttccgtcgac agtgaggtcg aagccgccct cagcgtggat
     4741 gccgaccagg tttccggccg ggatcgggat gccaccgagc accacacccc ggggaatctt
     4801 gacatcgaag cccagcttga cgttggccga ggcgtgcagc cccgtgctgc tgaggctgca
     4861 gctcatgtcg ccgtggagga tgtcgagcag gtccgcggcg atcgtgaagg cgaagccctc
     4921 gttgccgagc tgcgcctcga tcgaggccga caggccgagg aggtcgaggt tgccggagag
     4981 ggagaagatc accggcggaa gcttgccggt ccggatgccc cgggtgtcga ggaggtactg
     5041 ggggcccttg tttttgctcg cgtccgagaa ggtgaggacg ttgcccaact tcatcggctg
     5101 ggcgatggcg ctgtacgcgt agaggccctc gtcgctgacg ttgacttcga agcgccagtt
     5161 gttgccgaag aagtcgatgt cgcccttggc gtagaagccc tgcttccact cctcgccggt
     5221 ctcccggttg gtccagccgt tgggatcgat gaccacgcag atcgccagct tgtggagcac
     5281 gatctgctgc aggaaggaga gccagtccgg tggggcgacg aggacggcga gcatctccca
     5341 gaccgagatg ccggtgtcct tgtggacggt ccccagatcg aagtggaaga cggtgacgaa
     5401 gggctccgga ttgaacccgc cttcgatgtc caggtcgagg gtggcctctc cgatgatcac
     5461 cgacccgccc gcgcccaggg tgagcccggt ttccgccccg tagccgatct gcatgtagaa
     5521 gtagtggacg tcgaaccctt cgacgccgaa agcgttcttc cacgtggggc gatcgtcacc
     5581 ggagggcggc gcggagagcc cggcgaggcg gctcgcggtg tcgcgcacga ggctgtacgc
     5641 ggtcccgccc gtccggtggg gcgtggcgtc atggacgagg cggccgtcgg ctccaaggat
     5701 cggatccgcg gtgagcaggg tgtgcccggg ttcccggagc gcgtaggcca actggtgcgg
     5761 ggcgggccgt ccggcggctt cctcagcccc ggagccggcg gcgggcttga gcaccaggaa
     5821 gaggtggaac tccgtgccct tgagggaccc tccggccacg aactgcagca gggtcgcggg
     5881 cgcggagccg acggccggga cggtgaagtt ccacgaggtc tggagggaca cttcccgctg
     5941 tgccgggcgg aacgcgaggg tcaggccgcc gaagccgtcc cgggtcttct gctccgcgat
     6001 ggacgcagtg aactcggact cctggaccgg cgtggtgagc cgggccgtca ggtgtatgcc
     6061 actggcgtcc tggaacagct ctttcagggg cgccagcggg cccgtcaggc ccagttcggt
     6121 gaagaacgtg aggcccttgc cgagtgtgga cggcagggtt tcgggcgggc tggcgccggc
     6181 atcgacggtg acaccgtcgg cggagctgac ggcgagcccg gatttggcga aggtgatgcc
     6241 gtcgaggaag ccgagcaccg gggcccagtc cgacaccttt cccggggacc agacccagtc
     6301 cttctgccag aacgcggcgg cgaacagcgt gccgccctcc gccttcttgg ccgccttgcc
     6361 gaagaacgtc agctgcgcct cggcgaacgt cgccgtggtg ctgaccgtca gctcgtagct
     6421 cttgcccgag gccggctgcc ccgacagcgc gttccccagg cgtacggtca cggccggctg
     6481 gagcagggag acccgaggca ggccgtcggg cagcgccacg tcggcctggc aggccaggga
     6541 cttcaggagt gcggtcaggt cgacgtcggg gaagttcccg tcgagctcga aggcaccggg
     6601 gatggtccat gcggcggaga acttcacgga gtcgtcgccg ttgccgccct gggcggggcc
     6661 gagctcggca ttggccgaca aggtgccggt ggtccgcgag ccgccgttcg gggcgtcggc
     6721 tgtcccctcc agttgctgca ccgtgctgcc gccggcgccc tcaccgccct cgcgcgccag
     6781 cttcagcccg gcctcggtga ggctgatgcg ggcggcgccg atgtcgatgt gccagtcgga
     6841 ggagacggac gcttcgacct cgtacgtgcc tttcttgggt gtcgccgtgc cggagaattt
     6901 tttcagcgtc agctcgggca cgccggtgag gtcggcgcgc ggcaggaagt gatggagcac
     6961 gccccgcagt tcggcgggtg tgctctcgtc gagagcgagc tcgaacgtgc cgggcggtat
     7021 cttcgctgtg gtgtggaggc tgaagtcgcc cgcgacggtg aagtcgccgc gcccggtgac
     7081 ctccgccgtg cgggatggct ggagcggatt cgtgacggtc agggtggcgc cgacgccggt
     7141 cagggccagg tcgtcgccca tgatcggcca tgtggtgccg ggcgtggtct cgacggtggc
     7201 cttgacctgg gtcagggccg ctgccagccc gcccttgccg aacgctgcgg ggtcgatggt
     7261 ggcggtgatc tcggtgaggc ggacggagtc gccgagtgcg aagccctcct ggtcgacgag
     7321 ctggtggacc gccgcgccag tgccgttcca ggcggcgagg tcgaccgggg tgagccgctc
     7381 ggggaaccgg tcggcggtga gggcgaggac gccgccgccg gacggaagcg gagcactgat
     7441 cgtcgccccg accgccttgc ccatgaccag gtccgcggcc agccgtaagc cgtaggtgac
     7501 cggtgagccg ttcccgccgt cactacgcga cccggcccaa aggtagaagg acgcggagaa
     7561 cgccccgtcc ggctgcggcg tcgagcgcag tgcgatgtcg gtgcggacct cgccgccctg
     7621 ctcattgacg gggcggcgca cgaccggccc ggtcagcgac agggtcccgg gcctgggcct
     7681 gagcagcgcg gcgagtgcgc cgagctgctc cgggtcggtc acttgagggg catggaaggt
     7741 gaggccgggg tccagcgcgg ggtacccgtc gcggggaggc cgtcccaccg acgtgagcag
     7801 cagggcgggt ggtgcgggga aggcgagggc ggccaggtcg ctgccggcgg tggcggggaa
     7861 ggaggtggcg aaggtccagt ccgtgagcag ggcgagcggg acgtagaggg tggggtcctg
     7921 gtcgacgggg tcgaggccga aggtgatgcc gcggaccagc atgccggtga ggcccagacc
     7981 gtccacggtg agcgtgccgt gcacgaccac ggagtcgttg tcgtcctcgc ggtgcaggtc
     8041 ggtgatggcg agcgtgccgt cgagccaggt gcctatcagt ccggcggcgg gaccggtgcc
     8101 gaggtcggcg gccgggagcg tgagggtggt gccctgggtg tgccggccga tcagggcccg
     8161 gagttcttcg atcgtcatga aaggtcagcc ttcgagcttg gagtggatga ccgcgcgggc
     8221 gttgtcggtg agctccttgg cgatgtcggc acggtgcgcg tcgaccgcgt cctgcacccc
     8281 cttgatgatc ttctgcttga cctcgtcctt gttcagcacc tgctggatga tcttctccag
     8341 ggggctgggt tccttgagat ggatcgtgac ctgcaggttc tgcgtgggga cggcgagggc
     8401 gagcgcgtcc aggccgatgg tcagctcgtc ggcgtcgcgg ccggtgaagg acagggcggc
     8461 cgtcgccgag ggctggtcga tggtgacggt gatgtcgccc tggatcgtgt cgtcatcgcg
     8521 cgccgaggtg tgttccggcg cagcggcagc ggccgtgcca tccgggaagg acacgacacc
     8581 cgtgccggtg agggtgagcg ggggcggggg aaccttcgcg tcgccgggga gccggccgag
     8641 cctcagggtc gccccgatgc ccccgtcggt gagggcggcg tcctcgacgg ggatcaggac
     8701 gttggagatg cccttgatcg tgcctccctt gaagccgagg tcggccgtcg cgacgccgat
     8761 cttgtacgag ccgaggtcca tgtccccgag gccgtacggt tcgagcctgg ggtcggtcgc
     8821 cccgaggacg acggtcgggg gatagaagcc ggagcccgtg tcgttcacgg aggcccgcag
     8881 ccggtcgaag aggtagacct ccagcgggcc gtacttcgcc gggaagcccg tgtcgtccga
     8941 cggcagggtg cccgtcggca ccgcgccgag caccccgtcg agggccttgg ccagctgggt
     9001 ggtgaccgtg gcggagaact ggttacggaa ggagaggtcg gccagcgtgg cgaccagctt
     9061 cgcggtgagg gcctggccgg cctcggcgct gttgaaggcg tcggccgccg cctgcttcca
     9121 gatctgaagg gtggcctcgt cgatcgtgct gccttcgatg gtgagggact tctcgtcgag
     9181 gtggaactcc ggctgagacg cgacggagat cgactcgacg gtcagctgcg ggacgcggtc
     9241 cgctccggtg cccgtggcct cggcccggag cgtgacgtcg aaggcgaggt cctgggccgt
     9301 gacggtgaac cggccatggc cctcgatctc ctggcgcggc cactccacgc cctccaggcc
     9361 cttgaccacg accttcgtgg gcggcttcag gccgtccccg cccccgttcc cgggatcgtc
     9421 gatcaccacg acgtgctgca cgaggctgta cgtgccctgg acggtgatct gcttctgcca
     9481 tccgtgcgtg tcgtacgccg cggtggtgac ccggaccgtg ccccggtagc cgctggcggt
     9541 gggagtgagg ttctgcagct ccccgaccga ggcgttcggc agcccgtcga cggtgacggt
     9601 ggtcagggac agcgaggggt aggggtggtc ggcgtcgcgc tggctgttga aggggtcgag
     9661 gttgtccggg agatcgggca cgccgcggtc cttcgcgtcg tcgaaggcgg cgcccacgaa
     9721 gatccaccac gcggaaccga tcgtgtcccc gatcgtcttc ccttcgtcgc cgtcgacctg
     9781 cccgatgtcc cacgtgtcga cggtcaaggg attcagcgtg tggccgtcgg gcgtgcggac
     9841 gtccttgagg acggtaggca gccagtacgg gctgtccggg tcgttgatgc gcttgtacat
     9901 ggtggcgtag atgaagtcga gcagcttggt ggccacggga ctctccttca cgttccaggg
     9961 tcttggtgtg ctcggtcggg gcgggctgcc gtgccgctgc tcagaccccg cggtccacga
    10021 cgtgcagggc caggtcctcc agggcgtagc ggtaccgccc ggccgcgaac gggcgcagca
    10081 gctcgaccgc ctccagcgcc tgggcgcggg catcggtgta cgcgcgcgag aaccccgtgc
    10141 cgctgcggac ctcggcgagc gtgcgctcca gggcgccggg gccggtggct tcgccgcgca
    10201 tgatgcggct gaccgcatgc tccgggcccc actcctgcat ggcgtagatc aggggcagtg
    10261 aggcgcggcg ccgcaacagg tccatgccgc gcggcttgcc ggagccggcc tcgtcctggg
    10321 cgaggtcgaa caggtcgtcc atgacctgca gggcgcggcc gagccggcgg ccgtagaacc
    10381 cggcccggcg ggcgtcgccg ctgcgcccgt cggccagcgt caccccggcc tcggccgcga
    10441 gcccgaacag ggctgcggtc ttgcggtcgg cggtgcgcca gtaggtctcg gccagcagcc
    10501 ccgggtcctg ggagaggtcc gtgcgcagtt cgtcgagttc cccgcagccg atctggaagg
    10561 cggtgtcgag gacgagctcg atcaggccca tgtcgctgtc gcggtcgatc gtccgcacga
    10621 agccgcggat ggcctggacg aactgcaggt cgccgacgag caccgcggtc tccgtgccgc
    10681 gcgcggcgtt gaccgagggc aggccgcggc gcagggcggc gccgtccacg atgtcgtcgt
    10741 ggatcagcgt cgccacgtgc agcatctcca gggagacggc gccctggagc accttgtccg
    10801 gaagcggcgc ggggccgtgc accatgcggg cggcaagcag caggaccgcc gggcgcagcc
    10861 gcttgccgtg ccggtacagg gcccgttcac cgtcggtgag gtactcctgc tgcggggcca
    10921 gggcctggtc gaggaacgcc tcgaagcggg gcaggtcatc ggcgagcagg tgcgtccaca
    10981 ccccgccgag gacgtcctcg gcgggggcgg tcgtgtcgag gatgagcacg ggtcaggacc
    11041 gtccgtcctg cgggtgggcg tagccggcga tgccggtgaa cgcggcgtgc cggacggtct
    11101 cggcgatggc gtcctgcagc agcgtgtgca cgaaccgcat ggcggagagg cgctcgcggg
    11161 ccacatcggc ggcggtcccc tgccactcct cgtcgtcgat gctcagagcg aggtccggca
    11221 actcgaccgt cacgtccgcc ggtgcgccgt ccggcgtgag gcgggtggtg atgcggacgt
    11281 cctccagccg tgccgtgcac tccccggtgt ggagggtgtc cgtcgcctgc ggcccctgct
    11341 gcgcctcgtc gtggtaggcg gcggcgccga atccctggat gaccgcgcgg tcctcgtcgg
    11401 acagcgcgat ccagttctcc ggctcctccg gccggtcctc ctgctctgcc cggggcgctt
    11461 gaacctctcc gtcggcgaac gcgacgagtc cgttgtagcg cgccatttcc tcgggggaga
    11521 ccgccggagc ctccccggag tgccggtcga tggcggtgta gacgttgtcc tgggtcatca
    11581 ggatgatgtg cgagccgtcg ttgccggtct gctggaccga cttggagaag ctctcggcgg
    11641 ccgtggccgc agcctggagg cgctcggcct tggcggggtc cttccgcttg atccgggcgg
    11701 cttcggtggt gagcgcggtt tgcaccgtgg tccgctgctg ccaggcgtgc ccgttgtacg
    11761 tgacgccttc ttgcgggtcg tgccagtcgt tgaccggcgg ctggtcaccg gcggacggcc
    11821 gggcgtcgac gggcgcttcc gggtccgtgc tgtcgtaggt gtgctgcgac atgcgctggg
    11881 tgacgccctt ttcggcccat ttcttccgga ggttggagga gaccgaggag ttgccgttga
    11941 agatgtcgta gtagctctcg ttgtgcgtgc cgtactggac gaggacctgg cggccgttct
    12001 cggtcttcat cagtttggtg cggtgggaat cggcgcgctt cgtccacttg cggatggtcg
    12061 tgtcgtccgg gtggtcgggc gcgacggccc gggctccggc gggcagggtg ggctgtcgcg
    12121 cttccgccga gagcgggcgc aggtttccgg cggtgtcgat ctcgtggatc tcgtcgggcc
    12181 cggcgtcgag ggcgtagcgt cccgtgagga cgacggccgg aatgcggtgg gtgatgcggc
    12241 ggatgccgcc gcgggcctcc tcggcgaggg cttccggctg cccctcggcg cgcagttgca
    12301 gcgcaccgag ccggaagctc agcgagccgg tggcgtagtc gagggcgccg ggcatctcca
    12361 cgctgcgggc gagggggtag ccgggggtgt cggccaggtg gagcgtctcg gcctcggggc
    12421 cggtccggcg gtcgccggac tgcgggggct ggggcgtact caacgggtct cctgcggtgg
    12481 tcggacggac atgagagggg gcgggcggcg gagacgggcc cgccggaggg tgcggggacg
    12541 gctcaggaga cgacgaatgt gccgctgacc atgagcatcc cgtcgtggga gcccagttcc
    12601 ggcgacgtca gcttcacgga cacggtctgg ccctgcacgg tgatgtcggt gcccagcggc
    12661 gttttgagcg ggatgtcggc gctcttcttg ccctccagcg cgcccttcag gacggcgggg
    12721 gccgcgagag cgagcgcatt ggcgggtgcg gcgaccagct tggcggcgtc ggacttcggg
    12781 tacagcgaca cggagttgag gccgatcttc tccagcgtcg cggagagggc cgtgtccgtc
    12841 aggccggtgg ccttgaaggt gacgctgggt tcggccgggt cgaggacgac ctcgaagtcg
    12901 atcagtgcat ccacggtttt cgggacctcc tggagtgagg ggacgacgat gcgggacggt
    12961 ctcaggactc ctgggaggcc ttgaacaggg cgcaaccctt gccgggagcg acggggaaca
    13021 ggtcgacccc cacggaccag gggatgtcga agtcggccga gatcgtggtc gggtcgttct
    13081 cgtcgacctt cacgttctcg gccttccgga acgtggcttt ccaccagacc gaggcgaccc
    13141 cggtcacggt cttgtcgtca tcggccagct tcgtgttctg ccactggtcg agggcgccct
    13201 gcagcaggcg ggccatcagg tcccggccga cgagcaggaa gacctgcttg tccgacggcc
    13261 agtcggtggc cgcgccggcg ggggagccgg cggcgggggc gtggccggtt cctgcggtcg
    13321 ccgtgaggac caggtgggtc ccggtgacgt ccaggagcgc cggagtgagg gagacctgct
    13381 gtccgaagag gtcctggacg ggcagcttca gcccgctgac caggctcttc gcctggtcga
    13441 ggacgttcgg cacgatcacc ttctcgacca tgatccgcgt gtaggggtcc gggatctcgg
    13501 acagccgcac accgagcggg acgatggtga tggcgccggt gtcgaccttg gcctggaccg
    13561 cgactttgac ctcgctgacg ccttggggga gggcgtcgcc ggcgatggtg ccgttcactt
    13621 gcagctgcgg ggcgtggatc tgcaggactc ccgagtcggg caggtcggtg atgccctgcg
    13681 ccttgatcat ctgattccag agctcggtgt ccggtttctc gaggacgagc tcgggcggct
    13741 ccaggaagtc ccaggagaag gtgtactcga tgccttcgat ggtctcgggg ccgtaggcac
    13801 cctggaacac ggcccggtgc aggctgtgca gggacttgag gcccgagttg agcgcgtcgg
    13861 cgtggattcc gagtgcgagg tcatatggcg tttcggtcac gggagccgat ttaaggggct
    13921 gaacggccgg ggaaacaatc ggttcccggc catttgggaa tcggaatctg catcaacctt
    13981 gcctcacttg tgcaaggttg aattatggaa gtgcgcgcgg cgaaggaagg tgcggcgaag
    14041 gaacgcacgg cgtgacgcga cggattgtta tcgagtatcg ggcaaccctg aaggaacgcc
    14101 cgtccagaaa tccgttcgat attcgcccgg attcggtgtg cgggaaaacg gccctctgtc
    14161 gccggtgccg ggccccgggg agagggaaag gtgaaccagg gcccggcacc ggctccgcgg
    14221 ccgcccctac cgcttggtga cggtcacatc gccctggagc gcgagggcgt cgccgaagcg
    14281 cgagagggtc aggtccgccg gcgtcgcgtc gaggtggacg ggctcgacgt cgatggggat
    14341 ggccgggaac gcacccaccg ggaactcgac gccgcgcagc gcgctcccga cgatgccgcc
    14401 gaaggtcgac gacagggcgt cgacgaccca cgagaagatc atgctgacga tgtcgccgcg
    14461 cgtgggcacg agcttcaagt cgaaggaact cacctccgtg gcagtggcct tgagtgtcgt
    14521 ccccgacatg gccagggtga gcgtgacggt ggggtcgggc tccaggtgca ggtcgtagaa
    14581 cgcatcggtc gacccgcccc agaaccagtt gatcccggcg ctgccgctgc cggagacggc
    14641 catgtggacg cgcacggtcg gcacgtcgtc ccacaccacc tcgcaccgga cgttgtggat
    14701 ggagatgtcg gccttgtagt acgcgctgct gcccgcgaag tccttgtggt caccggcagg
    14761 gaaggtcttg ccctccaggt acgaggtcgc cacctgcgcg atccgctcga ccgcgtcccg
    14821 cccgagcagg gcgaagaagg ggctctgcgg ccagtcgtcg gggaaggggg gcttcggtgt
    14881 gccgtgctcc gccaggttca gggcggccac ggcccgctgc ggccggagga cggggacggg
    14941 cgccgacagc gacaggcccg ggatctgagg tgtgggcagc gtgatgcccg acagtacctt
    15001 ccgggactct tccaggacgt tggggagcac caccttgtcc aggaagatct tgtcgaccct
    15061 gctgggcgcg tcggcggtgg cgccgagggg gttgaggctg aagaggccct tgtccgaggt
    15121 cgtggcctgg acctggacgg tcaccgcgat gtcttcctcc acagggttcc cgtccccgga
    15181 gtcgaccttc acgtggacga ggttcagccg cacctggaac gtgctcccgt ccagcgaggc
    15241 gcgcagggcg gcgacgtgct cggccagctc ggcctcggtg acctggccgg cggcccaccc
    15301 cgacgtgaga cggtcccgta actcggcctc ggagtgcgcc tgcggggtca ggtcgaagac
    15361 cggtgcctcc ttgacgtccc aggagaccgt gaacgtgacg ccttccacgt cgaagggctg
    15421 cgagccggtg aagtactgcg gatagagctg cccgtagacc tccttggacg tcgagttcac
    15481 gacgtcggca ttgacgccca ccatgaggtc gcaattcatg cgtgctcctt tttccatttt
    15541 ttaccggcgg tcgcgtcggc ccatggaaaa gagcccggga ggcagcctcg acgggcgact
    15601 ccgggcacga cgaagcgacc agggcgacgg cataaatcat gggacgggtc cacggcccgt
    15661 tcaagaccgc cggagaaatc ccgacacacc accggcgctt tgttcctcac ggaaacatcc
    15721 ggccggtgcg cgttggcctc ctgctttccc tccggtgcgc ggtcctggac gggcgatgat
    15781 gtttggcgcg atcccggcac acttcctgac cagttgccgc ccgcctgctc agagccccgc
    15841 gactttggaa ctcgccgaca gctcgtagac cagggtgacc gtgcggcggc cgccgggcgg
    15901 gagggcgacg tcccagcggg cgatgccttc ggcgtcgacc gcggcgggcg ccggggagca
    15961 ggcttccttg cgcaggcgta cctccaccgc cgagacctcg gagaccggga tcctctcccg
    16021 caggacgacc acccgttcgt cctgctccgc gggggcggag aaccgggaca ggtacagccg
    16081 gaccgtgcgg gtgaccacgg tccgctgcat gattccggcg gagtcacggg actcctcggc
    16141 ctcccggacc acccggtggt cgtcgcagct gccgaaggcg agctcgacgg aggcgccggg
    16201 ggcggtgaag tccagcgtgc cgcggccgct gaatccgttg ccgcggacca ggtccacggg
    16261 cccggcgagc agcgcatggc cggaccggtt gtcgaaccgc accacctggg tgaccagcgg
    16321 agaaagctcg ggtgagcagg cgtactcgct gctcgcggcc gtggtgaaga cggagagcgg
    16381 cacccggtgg gcgcggccgt ctgcgggtac ggagaccgga gcgggggagc gcagcacccg
    16441 cgtctcgccg ccgtcgtcca ccccgggcag gccgagcacc gggaccgggc cgaggtcccc
    16501 gatctcctcc tcgcgcagtt cgacgtcgac cgtgcggcgc tccgcggcgg agcggtcctt
    16561 gagcgtcagc cggtcctcgc ccagccacgg tgggtcggtg gccagcgccg accgcgccgt
    16621 cgacaacgtc agccgtacgt ccgaccagtc ctcgccggtg cgctgccaga ccatcgcgtc
    16681 ggtctccagc gtcagggagc ccccgtcgag cacggcccgg taggcgggcc gccacagcgc
    16741 acacgcggtg aggtggctca atcgcagccc gaccggcccg gcggccgcgg cctccacggt
    16801 cagctcgaca tgaccgacca gctcggccgg ctcctcctcg gagaggccca tggcctgcca
    16861 gacctccctg agttcggagg cgatggcggc cagccgggcc tctacggcgc ggagttgctc
    16921 gccgtacgag tcgcgctcgt cgtccacccg gtccagttcg cgggcccagc ggggcccttc
    16981 ggactcgccg gagccggcgc cttcgccgat ctcccgcagc acatcggcgg cgaggcggcc
    17041 gagcaggtcg aggcgggcgt gcagccggtc gcgtcgctgc cccagagaga gctgctcctc
    17101 ttcgaggagg tgcacgcgcc ggcgcagggc tgagtcgtcg gcggacggca gcggctcgcg
    17161 cggcgtccag gtgcggacga tccgcacgtc cagcacggtc gtggggtgat cggcggtcag
    17221 ttcggcgtgg agggtgcggt cgacggccag ggcactgacc ggcccgagac gcagccgctg
    17281 gaccccggcc tccaggtcga gcacggcggt gcgctcgatg tgggcgcgat cctccatgca
    17341 ggtgacggcg gtgacgggga gggcgatcgg cttcggcgac gtggacatgg tgggtgtcag
    17401 ctcctgcggt tgccgccgac cagggccttg ccggccggga tgcggatctc gtaacggccg
    17461 tcgagggcgg cggtgtcgcc ggcgggcagc tccacccgcc agacgcgggt gcccggggcg
    17521 tggtgttcgg ggcccgtgcc gggctcgggt gccgtccagt cggcccgctc ctcgatgcgg
    17581 acgtccggtt cggaggtgac cggcacccgc tcgcggacct cgacggtgac gggcctcgcg
    17641 agccggttgg ccagttccac gtggacgcgg tggtcgagca cggtggtgtt gttgcgcagg
    17701 cccgaggtcg actcgtgcag gttcgtacga cgggtgaccc ggattccctc ggccggcccg
    17761 agccccaccc ggcggacgcc gccgggggcg agcgtgggca gtgcggcggt cagcaggaac
    17821 tcgtcgtcga ccgtgacctc caccgggccg gccagcagag cctggccggt ggcgttggag
    17881 agcaccgacg tcgcgtacac ggtctgctcc acggacggca cacagaggta ctcggtgcgc
    17941 agaccgaccg ggatctcggc gacggtgatg gtgtgccagg tgccgtccga cgggacgtcg
    18001 gcgcgggcgg tggcgtcgaa gcggtggtcg aaggagcccg ccgactcgcg gggccgcacg
    18061 gcgtgcccgg gcagtggcag cgcggccacc gcttcggcgc ggcggcggta ctcggccgcc
    18121 accgcgtcga agggagcgtc aggaaacagc ctgccccggc gacctccctg ctcgtccggg
    18181 ccgcacagga cgagggcggc gtagtcgagc tcggtactgc tcggctgcgg cggaccgggc
    18241 gccggaggcg gtggtggtgg cgcagcccgg ccgggagccg ccggcgccat gggggcggcg
    18301 gccctcgcga aggggctgcc gccgggatgc ggcatgcctc cgggttgcga gggggcgggc
    18361 tgcgaccgct cgggcatctc gccctcgaag tcctccgggt agtggccgcc tgcgccgccg
    18421 ggcgccgggg gcggtgcgcc gtagccctgc ggtgccgctg ccgccattgc tgccggcggt
    18481 ggcggaacgg gaccgggcgc ggagccgccc gtggcaccac cgggcgcgga accggcccac
    18541 acggcaccgg tcgtatcagg gcgagggcct gccgcctcgt acccggcgaa caggtcggag
    18601 agcccggccg ggggctcgcg ccagcccgaa ggcgcgggcg cgggctgacg gcgtccgatc
    18661 cggatcgagc ggagcctcgg caggtcggtg cggcgccgca ggtcggcggt ggccagggcg
    18721 atgcgcacgc cggtccagtc ctcgccggtg cgctgcgcga ccgaggcacg cagcaccaga
    18781 tcgccggtgc cgtcgccctg acggtaggcg aggcggtagg ccggcaccca gacggcgccc
    18841 ggcaccccgt actccagctc caggtccagc cccgtctccg cgtcagcggt gccgtcgagc
    18901 gtaaggaccg cggagaccgt ggcctccacg tgcgctgacg gtgcgtcggt ggaggcgcgg
    18961 tcgagcctgt ccgtggcgac gccgagttcg tgttcggcgc ggcgcagcgc ctcctccagc
    19021 tcgacgaggc gggcgtgcag ccccgtcagc cgctcctcga cgaagtcggc cagccccagc
    19081 cacgcgtcga ccggggtacg gcggtgcgat tcctcgcgcc tgcgggccgg tgggaccggg
    19141 cgcaggcccc tgacctcctc gatcaggctc agttgccggt cccggcggcc ctgcgcggcc
    19201 gcgcactcgt cgtacagccg ctcgacctcg cgctgcaacc cctcgggcgt gccggtgccg
    19261 agcggctcgg cctcgacctc cacccgcgcc tcggtgacac gcacccccgg cgcgcccagg
    19321 atccgggccc gcagcgaacc cgggtccagc gagcggggca gccccgtcac ccgcacccgg
    19381 ccgtccggcg gcacgctgcc ccgggccagt cggcggcaga ccgcgccctg cgcgtacacc
    19441 acgaccgaat cgagggtcga cccccacctc tgtgccgtct cagccgtcat gtgctgcgcc
    19501 ccccgccgcg tccgtgttgg gcgcagccta cgcccgagcg gtccgtgctt ccgcgccggt
    19561 cccgatgtcg gtgacccttc gtacgggcgg gggcatgagc acctccggcg aagtccgcga
    19621 gttcgcctcg ggccggggcc ccttacgtct ccagccgtgc cgccagttcc ccgggcagcg
    19681 catcccggtt ctgcgcggtc acgtggacca gttcgatgcc ggcgcgctgc ttgagggcgt
    19741 cggtgaacgg gtcgtggcgc gcgtgcacgg tggcgacgac gtcgatgtcg gccgtgagca
    19801 gttcccccac ggccttccgg aacgcggagc ttgccagctc catcccgccc agctcgtcga
    19861 tgagcgcgag atcccccggc gccgggcccg gtgacagcgg ggcgagggcc gacagggcca
    19921 gttcgtccat gacgcgcagg tcgacaccgt accggccgac gcgcggcggc ccggggaagg
    19981 cgacgtgcgc gagcacggcc cgcctgccgt ccagcgtctc cagcgcgaaa cccacgcggg
    20041 cggtgccctc ccggatctcc tccgtcgtga aaccgacggg cgtacgggtg cgcagcaggg
    20101 aggccagccg tcgcacggcg gtcgtcttgc ccacgcccgg ccgtccttcg agcaggatcc
    20161 tggtcggcat cgcgtcctcc gagggtgcgt gcagggcgtc gtccctgtga tcctcgcgcg
    20221 ggcgaggccg caggagtgag acggcgctcc ggcaagcgcg gccccccgtg ggcaggggcc
    20281 gtgcccacct ccccccttat tgcacatata ttgcagttgc gagcaagaag cagagggaga
    20341 cgtgctgtgg gtgcaccggt ggtgctgggg atcgagtcgt cgtgcgatga gacgggcgcg
    20401 ggcatcgtgc gggacgggaa actgctcgcg cacgtggtgg cgtcgagcat ggacgagcac
    20461 gcgcggttcg gcggggtggt gcccgagatc gccgcccggg cgcacctgca ctccttcaac
    20521 ccggtcgtgc gccaggccct ggaccaggcc gggatgcggc tcacccagat cgacgcggtc
    20581 gccgtcacca ccggccccgg cctgtccggc gcgctccagg tgggcctgtc cggcgccaag
    20641 accctcgcct acgcggccgg ggttccgctg tacggcgtcc accacctggc cgggcacgtc
    20701 gccgccgaca ccctcgagca cggcccgctg cccgaaccgt gcgtggtgct gatcgtctcc
    20761 ggcggccaca cctccctgct gctcgtacgg gacctggtcc gcgagccgat cctgcacctg
    20821 ggcgacaccc tggacgatgc ggcgggggag tgcttcgaca aggtggcccg catcctgggc
    20881 ctgccgtacc cgggcggacc cgccatcgac cgggcggcac gggagggcga ccggcgggcc
    20941 gtggccttcc cgcgcccgct gacccggccc ggcgacgacc cgtacgcgtt ctccttctcc
    21001 ggcctcaaga cggcggcggc ccgctgggt
//
