LOCUS       NZ_LNBE01000001        20825 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_1, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000001
VERSION     NZ_LNBE01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 441060)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 441060)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 441060)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 161077
            Orig. end                         :: 181902
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20825
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="3"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20825
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20825
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10825](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10825)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     misc_feature    114..129
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 23.11"
                     /tool="antismash"
     gene            363..1091
                     /locus_tag="AS857_RS00620"
     CDS             363..1091
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS00620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_144440671.1"
                     /transl_table=11
                     /translation="MAKTKPRIPNREANLRHGGYASDTCPRCGHVRSGSKPRHSVPELV
                     DSKQLRDAVGEIYHAMQADKKLAPDAAVMLGVLEARIGGTEYYLAACSSGLIDVGPYLK
                     EITYHKGAWRVANPDVPSVEHNPLKWRGEWKTVKGKQVDIPLTANGVEIPRIGQTCAAV
                     KLLLELGNRVAQSGKQGEGEVDYVRMSEQYYVGPENTTASREWHGKGATCSWTAHSCGP
                     CNIRIPYLICDVTSNWIENA"
     gene            complement(1147..2253)
                     /locus_tag="AS857_RS00625"
     CDS             complement(1147..2253)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644244.1"
                     /locus_tag="AS857_RS00625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_079109954.1"
                     /transl_table=11
                     /translation="MPAAVAVVVGAMAVGALVPPAASATAKPDTVQQGLNALVRSDALP
                     AALASVQDREGRTRTYTAGVGDLATGSKVPRDGQVRIGSNTKTFTAVVVLQLVGEGKIR
                     LDAPVDTYLPDLVRGEDIDGSRITVRQLLQHTSGLPNYVQYLGDDVRHYEPRDLLDIAL
                     QHKARFDPGKKWEYSNTNYVLAGLIVQKVTRRPLAEEIDQRIIKRIGLRHTYFPAPGDA
                     TIREPHPKGYYQESAGAPLRDGTELDPSWGWAAGQMISTNSDLNRFYTALLGGRLLPEA
                     QLAQMRTTVPAGEPFASGARYGLGLVSTPLSCRPGVYWGHGGSTTGYETRGGATDDGRA
                     ANVAVTLQPTDKEAMKRVESLVDTALCR"
     gene            2682..3023
                     /locus_tag="AS857_RS40800"
     CDS             2682..3023
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS40800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_245699517.1"
                     /transl_table=11
                     /translation="MNRRTYALAAAPIALLIGLAAPAAAADGPQPNTPKVPRAVCDTPA
                     KDAVLTAEEGIALRKCTNAKVAVKTWHWMGNYGSPYDVANAANGAGIGAGELITQEHAN
                     GLRPTFMFY"
     gene            complement(3251..3652)
                     /locus_tag="AS857_RS00635"
     CDS             complement(3251..3652)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS00635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058041100.1"
                     /transl_table=11
                     /translation="MGTKFIEVDESHKGQPGVEEGVKTIEVGGQTITTPIFVQRIDFDD
                     LAPEVTDNLTTVKFAVTVTEEMEDLTGEVDDDGSPMTEIKEIQVPKWLEVDLGPESLKQ
                     YEEVMAPFYAAARETEAPIVPAPRKRRKK"
     gene            complement(4133..4333)
                     /locus_tag="AS857_RS41785"
     CDS             complement(4133..4333)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039428.3"
                     /locus_tag="AS857_RS41785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SCO3870 family protein"
                     /protein_id="WP_058041101.1"
                     /transl_table=11
                     /translation="MKQVPFGALSAGLAVLGTALVSFAFQLRADGYEQYVQDVAYVAGT
                     AYFTAIMTVVTWVRARRRPLS"
     gene            complement(4377..5219)
                     /locus_tag="AS857_RS00645"
     CDS             complement(4377..5219)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379761.1"
                     /locus_tag="AS857_RS00645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_079109955.1"
                     /transl_table=11
                     /translation="MRTMLFPPRLTASAESVREAAHRRGLRTTQFTSLTLPDGRRGAEH
                     LHAGPRFADVVAPAYGIAPLEAPRDWLAGLPWELTRRDVRIMPIAEAYALRRPVFVKSP
                     NDKEIQAMVYADGSRLPGPDAVDRDTPVLVSDTVKFTAEYRLHCLDGKVHTGSRYAEGG
                     RMALGPLSDAARAFGDDVLAEAGTALPSAIVVDVGMVDGYWAVVEANAAWASGLYTASP
                     DRALDVILRAAGPLGDVAPRDRQFIRTPEPRGRGDAVTAEELRALFARMPGIQRPAHT"
     misc_feature    complement(5205..5220)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.73"
                     /tool="antismash"
     gene            5431..6159
                     /locus_tag="AS857_RS00650"
     CDS             5431..6159
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604551.1"
                     /locus_tag="AS857_RS00650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="WP_058041102.1"
                     /transl_table=11
                     /translation="MRTRLRLLTAVVTAGIAISGISACGLARKNFEDDTTLSQKITSVR
                     LESGSGGVTLRGKEGTAEVRLHRYVEYGGDRPDGVTHRVENGVLVLRGCSDDCSVAYTV
                     DLPAGIPVTGETSSGAIDLSRTGAVKVKTGSGAIDLRDVDGAVEVQASSGAITGRGLGS
                     GPIAAATSSGAIDLAVSSPQDVRAEAGSGAVTLTMPDGRYRVAAKSDSGGKDIAVTDDP
                     SGTHRIDLTTGSGAITVKRS"
     gene            6664..7269
                     /locus_tag="AS857_RS00655"
     CDS             6664..7269
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378881.1"
                     /locus_tag="AS857_RS00655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_058041103.1"
                     /transl_table=11
                     /translation="MQSLNTPKPIESGDAPEITRILARSFADDPMMLWLFPDEEGREGR
                     LAQWFDVMLTHKYGPLGHCERTDAAAAFWTPPGTAEQHPDEETLARINGLLGEHVARLG
                     EMLELVGEAAPREPHWYLAVLGADPAAQGSGHGAALLRSGLAKADAAGLPAHLESSKQS
                     NIGFYERFGFTVRDEIHVPGGGPTLWSMWREARGDADR"
     gene            complement(7353..8252)
                     /gene="lepB"
                     /locus_tag="AS857_RS00660"
     CDS             complement(7353..8252)
                     /EC_number="3.4.21.89"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /gene="lepB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953877.1"
                     /locus_tag="AS857_RS00660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="signal peptidase I"
                     /protein_id="WP_245699518.1"
                     /transl_table=11
                     /translation="MGNVGDVGDVAVGTQPEQDGPGEGPEESAGKTRRWRRTGRSAKAQ
                     RSFWKELPLLIVVTLVLALLIKTFLVQAFSIPSNSMQNTLQPGDRVLVDKLTRWFGSKP
                     ERGEVVVFHDPGNWMEGHPTPEPNVVQKALGFIGLVPSADEKDLIKRVVGVAGDTVECK
                     GAGPLKVNGKPLDEPYVFPGNTPCGMNPEVQFKVTVPKDRLWVMGDHRQDSQDSRYHQD
                     EDGGFVPVDDVVGRAIVIAWPVGRWDTLPVPATFDQKGIGKAAVAAPTAVAFAGAVPLV
                     LVRRRLLSRRKADGTTRN"
     gene            complement(8414..9967)
                     /locus_tag="AS857_RS00665"
     CDS             complement(8414..9967)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539434.1"
                     /locus_tag="AS857_RS00665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_058041104.1"
                     /transl_table=11
                     /translation="MTASTDRKWWAFTGVSLISFLGCIDLTIVNTAAPQIQADLGASVT
                     DLQLIVNMFIVALSMFMVTMGRLADTYGRRRVLYTGTVVFALASLGAGLAPDTGWLIAF
                     RFLQGAAGAVLYTSTGAIVQNAFPRSQRGRAIGALYGVNGLGLAVGPLLGGVIVSSVGW
                     HWVFWLNVPLIAVALVLCSFSVKESRDQDAPGLDWPGLVLISLGIPAVVLAFTLGDHWG
                     WSSARTLGLLAFATASLVAFHAVERRAATPIIDFRLFRHRAFLGAIVSDFALACFYATV
                     LFLLPLYLSGVRGYDGYATGALLLSCTAVMAALSPGVGRLTDRYGPRKLLAAGFAAFTV
                     SALMLARIAPDSSIGYLALALVAMGLGWAFILGPATVSALSAVPERQAGLAVGSSWTFH
                     NLGGAIGLAAAINLYRLQGERTLNAELAEHHAPTGPWTQAVVGEPEHATALLRQHTSLS
                     APEIGEVYGQVFSQGMQTAMWMVAGTSLAALAVLGLLGLSARRSTAAACEVAADNELAR
                     "
     gene            complement(10001..10825)
                     /locus_tag="AS857_RS00670"
     CDS             complement(10001..10825)
                     /GO_function="GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016572541.1"
                     /locus_tag="AS857_RS00670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_245699519.1"
                     /sec_met_domain="CDPS (E-value: 3e-37, bitscore: 123.0,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNNVTSIGHDINEFVGADGSDDTAGINGLSGQSRTGLVQHSYVL
                     DGRSKASFGERGKSLSLAGKRAVLLVSVGQRYHEGDKLRATVELIRRGGFQQLTIAVAD
                     TLQRTNYLGLSRQDAYDRALRAGDEWLQRNEKTLARISAISDVLRWDEALRDPVYPQFR
                     KHVEDAYEHDPRYRQALLRTVGKFLDRLTARDPEADVAAASRNCLSYLIEECPVIMPTW
                     AHRGYDYVIYPQPMTVAMAETRRLFVEDAYPGKAEWLPLKFKKRAATAQDII"
     misc_feature    10807..10824
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.89"
                     /tool="antismash"
     gene            complement(10828..11577)
                     /locus_tag="AS857_RS00675"
     CDS             complement(10828..11577)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS00675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_216823925.1"
                     /transl_table=11
                     /translation="MSQEDILSVVEGTTLAVHVPGFVHPEALASARRQLFDHPDRGSLS
                     QAAEFKRIGFAYSEISDSASRDRYHAEARTNIQRMRDLFAPYASPSDVLRLLLEETWAA
                     GAGLMHVEGRKAFIGICRYQDKNVDLNPHTDALERNLPAEHARRLVAQLSVNIYINIPR
                     TGGELELWGAEPGEDAYRHLMGGRTWGIDREKIGPPVEVVKPAPGDLLLLNPRQIHAVR
                     PSGDEPRITLGHFLGYYGTDRPLELWS"
     misc_feature    complement(11561..11577)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.14"
                     /tool="antismash"
     misc_feature    11710..11725
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     gene            complement(11894..12442)
                     /locus_tag="AS857_RS00680"
     CDS             complement(11894..12442)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS00680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058041106.1"
                     /transl_table=11
                     /translation="MLYVCGFERIGVVASDMYFVDPDPVPGQEGAERGVRLEVRVLRSG
                     DLPGGIYASRPIVVDRPVWRADLLESVAGPSGSLDRAHHHPRFDGWEPGARVFVEELSA
                     APLDWVAGRLADLDTLLTEADVPASEAGPGDAADLRNAVPDIMAAVRRLLDGVAKGELA
                     RPPAGLAEEDIADARVSWL"
     misc_feature    complement(12479..12493)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.04"
                     /tool="antismash"
     gene            complement(12683..13327)
                     /locus_tag="AS857_RS00685"
     CDS             complement(12683..13327)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093243.1"
                     /locus_tag="AS857_RS00685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA alkylation repair protein"
                     /protein_id="WP_058041311.1"
                     /transl_table=11
                     /translation="MAELAGLDDPKTRAVNEKHGDDHGVNLGKLRALAKRLKTQQELAC
                     QLWETGDTAARLLATLICRPKAFERDELDGMLREARTPKVHDWLVNYVVKKNPHSEELR
                     LAWSADPDPVVASAGWALTTERVTKHPEGLDLAGLLDVIDAEMKDAPDRLQWAMNHCLA
                     QIGIEHAEHRTRAIDIGERLEVLKDYPTSPGCTSPFAPVWITEMVRRQRDE"
     gene            13952..14863
                     /locus_tag="AS857_RS00695"
     CDS             13952..14863
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574238.1"
                     /locus_tag="AS857_RS00695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lysozyme"
                     /protein_id="WP_058041108.1"
                     /transl_table=11
                     /translation="MPEHHSGPGRPHPPTAAAILLSLLTVLTLLVTLPGTAAAVPGNDI
                     RPTHPEQDWMGSTIRAHEGERGPVAKGSSLPDPSLPDLPLAGPPASASVSAGVKGVDVS
                     SHNGNVSWSKLWSSGIRFAYAKATEGTSYTNPHFTHQYNGPYDAGMIRGAYHFALPGNS
                     SGAAQADYFVDHGGGWTDDGWTLPGVLDMEYNPYGSTCYGLSTGRMVDWIEDFLDAYRD
                     HTGRDAVIYTSTAWWKKCTGNHGGFGKYNPLWVPRYGSSSGELPNGWDFYTFWQHTNTG
                     PTVGDHDRFNGSYDRLEALAYG"
     gene            15101..15421
                     /locus_tag="AS857_RS00700"
     CDS             15101..15421
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020127361.1"
                     /locus_tag="AS857_RS00700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058041109.1"
                     /transl_table=11
                     /translation="MRARDKAAVSALRATLAALDNAEAVPVDEAQLRGVALEHAPVGAG
                     ATEAPRRELSEHSMVDVVRAEVTERLEVAAQLTAPAHADRAAQLRAEAAVLLRFLDGPR
                     AA"
     gene            complement(15563..16369)
                     /locus_tag="AS857_RS00705"
     CDS             complement(15563..16369)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS00705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_144440672.1"
                     /transl_table=11
                     /translation="MRPTPALILRRRVTQLDTADPTAGTDAGLRHFTSVMTGWTEPEVT
                     ALAGGPAVLAPSEVEEADEMADGCLLLARLARDQECLVRGPLDRTRQHWIGTSPHELMP
                     HRRPAPDRERFTEPGGDSGPPSTKPFHLGLYTSTARRDGPGMWRMYLDLGSSLHPLPWQ
                     TWELPVTDLTADVLDIGGACDWAAFVGRYPLVRGDDVYPDWAKAARDFAGVHMTLRAIV
                     AAQGFRIPVADGLAAAPFWDIESVLWLRWCFSAPRLVEITQPDAGP"
     gene            complement(16432..16941)
                     /locus_tag="AS857_RS00710"
     CDS             complement(16432..16941)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS00710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_144440673.1"
                     /transl_table=11
                     /translation="MRTWRTAVAAASMAVLALVGGTSPAAAAGDPSAPATVPEGRDADG
                     GSVDAGKAGSDADASAVIRCQGQVQRPHNSTHVPGTINVVVTVSCSAPVPEITTRAALY
                     KNGHLVKQSTPRTQRNTRSAQNNAAVSCSPGKYRGWMYFKVVFPPGYNPPTDSANALGQ
                     EVSLSC"
     gene            complement(17088..18257)
                     /locus_tag="AS857_RS00715"
     CDS             complement(17088..18257)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091409.1"
                     /locus_tag="AS857_RS00715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_058041112.1"
                     /transl_table=11
                     /translation="MFTTPRIAIAGAGLGGLVCARVLQQHGVPVTVYEGETHPHSRSQG
                     GTLDIDEDTGQAALRAAGLFDQFLALSRPEGQEWRLYDRHASLIRHDQAGEGDLSRPEI
                     DRGQLRALLLDSLAPGTVRWGHSVRAVTPLPHGAARLHHHDRTVEEFDLVIGADGAWSR
                     VRSALSDAQPSYAGVTMVEAHFDDVDHRHPGIAHLVGRGTMTAKAGRRSLVLQRNSNGH
                     VRAYVTFRGPQDWHAGLDLADTEAVRARLLAQYQGWHESLLDILRENEGGFINRPVFAL
                     PVPHSWQRVPGITLLGDAAHLMPPAGVGANLALLDGAELARAVIDHSAIDAALDAYESV
                     MLPRAADNAKTAEQMLTTLMPDTDSDEAAFPETLWPSGTLPDPHPAHTS"
     misc_feature    complement(18251..18265)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.87"
                     /tool="antismash"
     misc_feature    18262..18275
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.68"
                     /tool="antismash"
     misc_feature    18330..18344
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.17"
                     /tool="antismash"
     misc_feature    18467..18484
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 23.05"
                     /tool="antismash"
     gene            18495..19103
                     /locus_tag="AS857_RS00720"
     CDS             18495..19103
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091408.1"
                     /locus_tag="AS857_RS00720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_058041113.1"
                     /transl_table=11
                     /translation="MTGTATGRRERKKAQTRQALADAAVRLFTERGFDDVGVREVAEAA
                     DVSLSTLFKHFPSKEALVFDLDADVESALVAAVSDRAPGQSVLHALRDHMVHARTAVRT
                     DDPTYVLVESTPALRDYARRMWSRHEKTLAATLAEATGLTPEDPAVAGLARFTLEAPSI
                     ARASEDPPRTMRDLFNLLEHGWATTLLARQGEGCPGRDG"
     gene            19251..19856
                     /locus_tag="AS857_RS00725"
     CDS             19251..19856
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103541.1"
                     /locus_tag="AS857_RS00725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_058041114.1"
                     /transl_table=11
                     /translation="MADEPERTSQPAKPTRRLDARSLRGLAHPLRMRIFELLSLDGPAT
                     ATGLSERLGENTGTVSWHLRHLAEHGFIEEEAERGTKRERWWRRAGGTNELHTTDFRDD
                     PDSRGALSVYLHELVHQYFGRVTNYLNEDWDDAWRGAGTVSDWRDLRMTPDQLAALNAD
                     LMAVIARHTPAPDTEPDPDALPVVVQLQSFPRKKRGTA"
ORIGIN
        1 gtgaaattcg ggcgcacact atcaacggcc gaatccggag cggcgatgat caagccaggt
       61 ggtgcgcggg aggacgcgga gctttcgtcg gattccacgg tgaaatccaa ctgttgaccg
      121 ttttgtcgat atgtgtgacg agctgtcacc aatgcctgcc ggaaagctgc tgcgacctgc
      181 ggtggatcgc gaccgggtgg gccgaaagcg gacccgggga tgaccttcgc ggggtggtgg
      241 cgtcgcgacc gagcgttacg tgatcacgtc caaaagtagt cgctgcgctt cggtgcgcgc
      301 ccggtggccg ggccgccacg tgctattttc cccgcgccac ggaaaactga cggagggtgg
      361 acatggcaaa aacaaagccc cggatcccga atcgggaagc gaatctccgg cacggcggat
      421 acgcgtccga cacgtgcccc cgctgcggtc atgtgagaag cgggagcaaa ccgcgacaca
      481 gcgttccgga acttgtcgac agcaagcaac tgagggacgc cgtcggcgag atataccacg
      541 cgatgcaggc ggacaagaag ctggccccgg atgctgccgt catgctcgga gtgctggaag
      601 cgcgaatagg cggaaccgag tactaccttg cggcttgcag cagcgggctc atcgatgtcg
      661 gcccatacct caaggagatc acctaccaca agggtgcctg gcgagtcgca aacccggatg
      721 tccccagcgt cgagcacaat ccgctgaagt ggagggggga atggaagacc gtcaagggga
      781 agcaggtgga catccccttg acggcgaacg gcgtggaaat accgcgtatc ggccagacct
      841 gcgcggccgt caaactgctg ctcgaactgg gaaaccgcgt tgcgcaatcc ggaaagcagg
      901 gcgagggcga ggtggactac gtccgcatga gcgagcagta ctacgtgggc cccgagaaca
      961 cgactgcctc acgtgagtgg cacgggaagg gcgcgacctg ctcatggacg gcgcactcgt
     1021 gcggcccgtg caacatccgc atcccctacc tgatctgtga cgtcacttcc aactggatcg
     1081 agaacgcctg agcgcttcgc tgcgaacgcg gtccgggcgg gccctgtccc tgcgggcccc
     1141 tccggttcag cggcacaggg ccgtgtccac gaggctttcc acgcgcttca tggcttcctt
     1201 gtcggtcggc tgcaaggtca ccgcgacgtt ggcagcgcgg ccgtcgtcgg tggcgccgcc
     1261 ccgggtctcg tatcccgtgg tgctgccgcc gtggccccag tacacgccgg ggcggcacga
     1321 cagcggcgtg ctgacgagcc ccagtccgta gcgggcgccg gaggcgaacg gttctccggc
     1381 ggggacggtg gtgcgcattt gggcgagctg ggcctccggg aggaggcggc cgccgaggag
     1441 cgcggtgtag aaccggttga gatcggagtt ggtggagatc atctgccctg ccgcccagcc
     1501 ccaggagggg tccagttccg tgccgtcgcg caaaggcgcc cctgctgatt cctggtagta
     1561 gcccttgggg tgcggttcgc ggatggtcgc gtcaccgggg gccgggaagt aggtgtggcg
     1621 cagcccgatg cgcttgatga tgcgctggtc gatctcctcg gcgagagggc ggcgcgtgac
     1681 cttctggacg atcaggccgg ccagcacgta gttggtgttg ctgtactccc acttcttccc
     1741 gggatcgaag cgggccttgt gctggagggc gatgtcgagg aggtcgcgag gctcgtaatg
     1801 ccggacatcg tcgccgaggt actggacata gttgggaagc ccgctggtgt gctgcaggag
     1861 ctggcggacg gtgatgcggg atccgtcgat gtcctccccg cggacgaggt ccggcaggta
     1921 ggtgtcgacc ggtgcgtcga ggcggatctt cccctcaccg accagttgca gtacgacgac
     1981 ggcggtgaac gtcttggtgt tgctgccgat ccgcacctgt ccgtcccggg gcaccttcga
     2041 accggtggcc aggtcgccga cccctgcggt gtaggtacgg gtgcggcctt cacggtcctg
     2101 gacgctcgcc agcgcggcgg gcagggcgtc ggagcgcacc agcgcgttca ggccctgctg
     2161 aacggtgtcc ggcttggcgg tggcagacgc cgcgggcggc accagggcac ctaccgccat
     2221 ggcgccgacg accaccgcga ccgcggccgg cacggctcta cgccggccac cccgccgctg
     2281 cccggacgaa cgaaacctct gccacgcctg ctcacgcacg agccaactcc tacggaagac
     2341 atcgggaaac cagcgatcca tgtgctggat atgtgccggg cccaacattt ccggatgcaa
     2401 ccacctggga cgatcctgct acccgcccag gccgaggtgg ggctaccccc ccggtgccga
     2461 ccgacgggac gggaccgcgg acggggccgg acgaccggct ccgtcccgca ccgacgctgc
     2521 ctggcacggc gggtgccgct ggggtcgacc ctttcggccg ctcaacatgg cggccgtcac
     2581 ccgaacggcg tactcgatgt gcgagggcga ccaggccacc gtagggtcgt gggtccaggt
     2641 gtccgctctg tcgaacatct gatcccctca tggaggtcac catgaaccgt cgaacctacg
     2701 ccctcgcagc agcccccata gccctgctga tcggcctggc agccccggcg gcggccgcgg
     2761 acggcccgca gccgaacacg cccaaggtcc cgcgagcggt ttgcgacacg ccggccaagg
     2821 acgccgtgct caccgctgag gaaggcatcg cgctcaggaa gtgcaccaac gcgaaggtcg
     2881 ccgtcaagac ctggcactgg atgggcaact acggctcgcc gtacgacgtg gccaacgcgg
     2941 ccaacggcgc gggaatcggc gccggcgagc tcatcaccca ggagcacgcc aacggcctcc
     3001 gcccgacgtt catgttctac tgatccccct tcagtggaac acgctcgcca cggcggccca
     3061 tcccgcatga ccgagcgatg atcgccgacc gcacgccatc ctggcgtgcg gtctcggcgt
     3121 atacaggggc tgcggacact ttcaccggag gccggccgat tcccccgtgc gaagccgacg
     3181 ctgcggcacg acgcacacat ggcctcatac aacgaagccc ctctcctgga gacaggagag
     3241 gggcgcaaga tcacttcttc cggcgcttgc ggggcgcggg cacgatcggt gcctcggttt
     3301 cgcgggcggc ggcgtagaag ggggccatta cctcctcgta ctgcttgagg gattcgggcc
     3361 ccaggtcaac ctcgagccac ttgggcacct ggatttcctt gatttccgtc atcggggaac
     3421 cgtcatcgtc cacctccccc gtcaggtcct ccatttcctc ggtcaccgtt acggcgaact
     3481 tgaccgtggt cagattgtcg gtcacctcgg gggcgaggtc gtcgaagtcg atccgctgga
     3541 cgaaaatcgg cgtcgtgatc gtctgaccac cgacctcgat cgtctttacc ccttcttcca
     3601 cgccgggctg acccttgtgg gattcgtcta cctcaatgaa tttggtgccc atggatggcg
     3661 ccactccttt ttgctggtgt gtgcgcaaag tctagtggag caggggctgt tgacgccaat
     3721 tcagaggaga gagccggtcc ctcctcgggc ggggcgaccc ggcgctcccg ggaaaccgcc
     3781 ggcaatgtga ccccgagctc ttttcgtatt ggagaagcgg accggcggag agcggtcgct
     3841 cttctcaggg gccgtacgga ttcctaccct ttggtgagaa ctgtgccccg gggaagggag
     3901 acccccagga agaccagagt ctggtgaacg gacaagggag gagggaactc ggagccctgg
     3961 tagggcggtg tccgtctccg ggaacgccgg cccggcccgg cccgactcag ctcgcgcatc
     4021 gcgtgtccgc gaccgcgctg acgtgggccg gtttgagggc gtgtccggtg agtcgagtga
     4081 caggacccac cagacagacc tcgtcatggc gcgtgcgcca tgaccgaacg actcagctca
     4141 acggccgccg gcgagcacgg acccacgtca ccacagtcat gattgcggtg aagtatgccg
     4201 tgccggccac ataagccacg tcctggacgt actgctcgta gccgtctgcg cgcaactgaa
     4261 acgcgaagga tacaagtgca gtcccgagta ctgcgagccc agccgacagg gcgccaaatg
     4321 ggacctgttt cattccatgt cctcccaacg gtgaaacgac ctggtcacca cccaccctac
     4381 gtgtgcgctg gcctctggat gccgggcatc cgggcgaaga gcgcccgcag ttcttccgca
     4441 gtgacagcgt ccccgcgccc ccggggctca ggtgtacgga tgaactgccg gtcgcggggc
     4501 gcgacgtcgc cgagaggtcc ggcggcgcgc aggatcacgt cgagagcccg gtccggagag
     4561 gcggtgtaga gcccgctggc ccacgccgca ttggcctcga ccactgccca gtacccgtcg
     4621 accatgccca catcgacgac gatggcggag ggcagcgctg tgccggcctc ggcgagcacg
     4681 tcgtcgccga aggccctcgc cgcgtccgac agcggtccca gcgccattcg tcctccctcg
     4741 gcgtaccggc tccctgtgtg cactttcccg tcgaggcagt ggagccggta ttcggctgtg
     4801 aacttcaccg tgtcgctgac cagcaccggc gtatcccgat ccacggcgtc cggcccgggc
     4861 agacgtgatc cgtcggcata gaccatcgcc tgtatctctt tgtcgttcgg ggacttgacg
     4921 aataccggcc gccggagggc gtaggcctcg gcgatcggca tgatccgtac atcgcgtcgg
     4981 gtgagctccc atgggagccc ggccagccag tcgcgtggtg cttcgagcgg cgcgatgccg
     5041 taagccggtg ccacaacatc cgcgaaacgc gggcccgcgt gcaggtgctc ggcgccgcgt
     5101 cggccgtcgg gcagtgtgag tgacgtgaac tgcgtggttc gcaggccgcg tcggtgggcc
     5161 gcctcgcgga ccgactcggc ggaggcggtc agtcgagggg ggaagagcat ggttctcatg
     5221 gcagaggatc atgtctcatc gctcgtcatg cctgttcgga ctccccgccg agtgactccg
     5281 cgttcgggtc accgttcgag atcaggccat aaggaacagg ccaagatccc gggggtttac
     5341 cccatggccg tactccgacc ccggcgcata ggctctctcg gcgtccccca gtgggttctc
     5401 ctgacgacct ctcaagaatg gtgatagtcc atgcgtacgc gccttcgtct gctcactgcc
     5461 gtcgtgacgg ccggaattgc catcagcggg atcagcgcgt gcggactggc caggaagaac
     5521 ttcgaggacg acaccacgct gtcgcagaag atcacctccg tgcgactgga gagcggctcc
     5581 ggcggggtca ccctgcgcgg caaggaaggc actgccgagg tgcgcctgca ccgttacgtg
     5641 gaatacggag gagaccgccc ggacggtgtg acacaccgcg ttgagaacgg agtcctggtg
     5701 ctccgtggct gcagtgacga ctgctcggtg gcctacacgg tcgacctgcc ggcaggcatc
     5761 ccggtgaccg gtgagacctc cagcggcgcg atcgatttgt ccaggaccgg cgcagtgaag
     5821 gtgaagaccg gctccggcgc catcgatctc agggacgtcg acggggcggt cgaggtgcaa
     5881 gcctccagcg gcgcgatcac cggacggggg ctcggaagcg ggcccatcgc cgccgcaacc
     5941 tccagcggag ccatcgacct ggccgtgtcc tcaccccagg acgtccgggc ggaagccggc
     6001 agcggcgccg tcactctcac catgcctgac gggaggtacc gggtagccgc caagagcgac
     6061 agcggaggca aggacatcgc cgtcaccgac gacccgtccg gcacgcaccg gatcgacctg
     6121 accaccggca gcggcgccat caccgtcaaa cgctcctgac gagcagcctg acacggaccg
     6181 gcggatgacg gccgggccta cgcgtacgag tgcaggtgcc cgggcgggag ctcgacggcg
     6241 ccgcccgggc aagccgcaga ggtgctccag gggtgcctgg atcgagcgtc agaaggcccg
     6301 aaacgccgag ctggtcacgc tgacttcgtg ctgctgggtg tgtagggcat gaggaggccg
     6361 acgactccca gcagtgcggc gatccacaag gcggcgacat ggccgcctcc ggcggcgaga
     6421 gcccagccgg caagcagggg tcctgcggca ttgcccacgt tgaaggccga gatgttggcc
     6481 gccgaggcga gtgtccccgc gccctgggca gtgacacgaa cccgctgatc ccgccgttcg
     6541 tccgggtcgc gacggccgcc tgccgtgact gagggcggac cacagctgtt gccgtccgtc
     6601 agccgtgggg gatcggtaga acgaccgatg tgctggaagg atcttggaac ctacgttggc
     6661 cgcatgcagt cactgaacac acccaagccg atcgagagcg gcgacgctcc tgagatcaca
     6721 cgcatcctgg cccgctcctt cgcggacgac ccgatgatgc tgtggctctt ccccgacgaa
     6781 gaagggcgtg agggccgcct cgcccagtgg ttcgacgtca tgctcacgca caagtacggc
     6841 cctctcggtc actgtgaacg caccgacgcg gcagcggcct tctggacccc gcccggcacg
     6901 gccgagcagc acccggacga ggagacgctg gcccgtatca acgggctgct gggcgagcac
     6961 gtggcccggc tgggagaaat gctggaactg gtcggcgagg cggccccacg ggaaccgcac
     7021 tggtacctgg cggtgctcgg cgccgacccg gcggcccagg gcagcggcca cggcgccgcc
     7081 ctgctgcgtt cgggcctggc caaggcggac gcggccggac tgccggccca cctcgaatcg
     7141 tcgaagcagt ccaacatcgg cttctacgag cggttcgggt tcaccgtgcg ggacgagatc
     7201 cacgtccccg gaggcggccc gacgctctgg tcgatgtggc gggaggcccg cggggacgct
     7261 gaccggtagg cccggacctg gccgaggggc tgctggtcca gtcgacgggc cggcctcggg
     7321 gccggcctcg gtggagcgcc tcggcgggta ggtcagttcc gtgtcgtgcc gtcggccttg
     7381 cggcgggaga gcaggcgccg gcgcaccagt accaggggga cggcgccggc gaaggccacc
     7441 gcggtggggg cggcgacggc cgccttgccg atgcccttct ggtcgaaggt ggcggggacg
     7501 gggagggtgt cccagcggcc gacgggccag gcgatgacga tggcgcggcc caccacgtcg
     7561 tccacgggga cgaagccccc gtcctcgtcc tggtggtagc gggagtcctg cgagtcctgc
     7621 cggtggtcgc ccatcaccca cagccggtcc ttgggcaccg tgaccttgaa ttggacttcc
     7681 ggattcatgc cgcacggggt gttgccgggg aagacgtagg gctcgtccag cggcttgccg
     7741 ttgaccttca gcgggccggc gcccttgcac tcgacggtgt caccggcgac gcccacgacc
     7801 cgcttgatga ggtccttctc gtcggcggac ggaaccagcc cgatgaagcc cagcgccttc
     7861 tgcaccacgt tgggctcggg cgtcggatgg ccctccatcc agtttcccgg gtcgtggaag
     7921 acgacgacct cgccgcgctc gggcttcgag ccgaaccacc gggtcagctt gtcgaccagg
     7981 acccggtcac ccggttgcag ggtgttctgc atggagttcg aggggatcga gaacgcctgc
     8041 accaggaacg tcttgatcag cagcgccagc accagcgtga ccacgatgag cagcggaagt
     8101 tccttccaga acgaccgctg cgccttcgcg gaccttccgg ttctcctcca ccttctcgtc
     8161 tttcccgccg actcctcggg cccctcaccc gggccgtcct gttccggctg agtgccgacc
     8221 gccacgtccc ccacgtcccc cacgttcccc acgtcccctc ctcgctccgg ccaccgcccg
     8281 ctccgtccgc ggtgcgggtc ccggcacccc tggtcgagcg ggatgttccg gtgatgtcac
     8341 cttatgcgaa ggggagtgac ggcggccgtc cgcggaatcc gctccccggc cctcccgcgc
     8401 gcccgccgga tccttaccgg gccagttcgt tgtcggccgc cacctcgcag gcggcggccg
     8461 tggaccggcg tgccgacaga ccgagcaggc cgaggacggc gagggcggcc agcgaggtgc
     8521 cggccaccat ccacatcgcg gtctgcatgc cctgggagaa gacctggccg tagacctcgc
     8581 cgatctccgg tgcgctgagg gaggtgtgct gccggagcag cgccgtggcg tgctccggtt
     8641 cgccgaccac ggcttgcgtc caggggccgg tgggggcgtg gtgttcggcc agttcggcgt
     8701 tcagcgtgcg ttcgccctga aggcggtaga ggttgatggc ggccgccagt ccgatggctc
     8761 cgccgaggtt gtggaaggtc caggaggatc cgacggcgag tcccgcctgc cgctcgggca
     8821 cggcggacag cgcggagacg gtggcggggc cgaggatgaa cgcccagccg aggcccatgg
     8881 ccaccagcgc gagggcgagg tatccgatgg agctgtcagg ggcgatccgg gcgagcatca
     8941 gggccgagac cgtgaacgcg gcgaagccgg cggccaggag cttccttggg ccgtagcggt
     9001 cggtcagccg gccgacgccg ggggagagcg ctgccatgac ggcggtgcac gacagcagta
     9061 gcgctccggt ggcgtaaccg tcgtagcccc gtacgccgct gaggtagagg ggcagcagga
     9121 acagcaccgt ggcgtagaag caggccaggg cgaagtcgga gacgatggcg ccgaggaagg
     9181 cgcggtggcg gaagagccgg aagtcgatga tgggggtggc cgcgcggcgt tcgacggcgt
     9241 ggaaggcgac gagcgaggcc gtggcgaagg ccagcaggcc cagggtgcgg gcgctggacc
     9301 agccccagtg gtcgccgagg gtgaaggcga ggaccacggc ggggatgccc aggctgatca
     9361 gcacgagccc gggccagtcc aggccggggg cgtcctggtc gcgggactcc ttgacgctga
     9421 aggagcacag gaccagggcg acggcgatga gggggacgtt cagccagaac acccagtgcc
     9481 agccgacgga ggacacgatg acgccgccga gcagcgggcc gacggccagg cccaagccgt
     9541 tgacaccgta gagggcgccg atggcacggc ctcgctgcga ccgggggaag gcgttctgga
     9601 cgatggcgcc cgtggaggtg tagaggacgg ctcccgcggc gccttggagg aaccggaagg
     9661 cgatgagcca gccggtgtcc ggggcgagtc cggcgccgag ggaggccagg gcgaagacga
     9721 cggttccggt gtagaggacg cgacgccggc cgtaggtgtc agccagccgc cccatcgtca
     9781 ccatgaacat cgacagggcg acgatgaaca tgttgacgat gagctggagg tcggtcacgg
     9841 aggcgccgag atcggcctgg atctgtgggg cggcggtatt gacgatggtg aggtcgatgc
     9901 agccgaggaa gctgatgagg ctgacaccgg tgaatgccca ccacttgcgg tcggtcgatg
     9961 ctgtcacggt gattttcccc tacaccgggt cgaagggcgg ctagatgatg tcctgggcgg
    10021 tggcggcccg tttcttgaac ttcaggggga gccattccgc cttgccggga tatgcgtctt
    10081 cgacgaacag ccggcgagtc tccgccatgg ccaccgtcat gggctgcggg taaatcacat
    10141 agtcgtatcc gcggtgcgcc caggtcggca tgatgacggg gcattcctcg atgaggtagg
    10201 acaggcaatt ccgggacgcc gcggcgacat ccgcttccgg atcgcgtgcc gtgagacggt
    10261 cgaggaactt tccgacggtg cggagcaggg cctgccggta gcgcgggtcg tgctcgtagg
    10321 cgtcctcgac gtgcttccgg aactgcgggt agaccggatc gcgcagtgcc tcgtcccagc
    10381 ggaggacatc gctgatcgcg gagatccgcg cgagcgtttt ctcgttgcgc tgcagccact
    10441 cgtcccccgc cctgagtgcg cggtcgtaag cgtcctgccg ggaaagcccg agatagttgg
    10501 tgcgctgcag agtatcagcc acggcaatcg tcagctgctg aaacccgccg cgtcggatga
    10561 gttccaccgt ggcgcggagt ttgtcgccct cgtgatagcg ctggccgacg ctcaccagca
    10621 ggacggcccg tttcccggcg agagagagcg acttgcccct ctccccgaaa ctcgccttgc
    10681 tgcgaccgtc gaggacatag gagtgctgga cgaggcctgt gcggctctgt ccactgagac
    10741 cgttgatgcc ggccgtgtcg tcggagccgt cggcaccgac gaattcgttg atgtcatggc
    10801 cgatgcttgt gacgttgttg ttcaccgtca gctccacagt tcgagcgggc ggtcggtgcc
    10861 gtagtagccg aggaagtgcc ccagagtgat gcggggctcg tcccccgacg ggcgcacggc
    10921 gtggatctga cgcgggttga gcaggagcag atcacccggg gccggcttga cgacctccac
    10981 gggcgggccg atcttctcgc ggtcgattcc ccaggtccgt ccgcccatga ggtggcggta
    11041 ggcgtcctcg ccgggctcgg ccccccacag ctccagctct ccgccggtac gcgggatgtt
    11101 gatgtagatg ttgaccgaga gctgcgccac gaggcggcgc gcgtgctcgg cgggcaggtt
    11161 ccgctcgagg gcgtcggtgt gcggattgag gtcgacgttc ttgtcctggt agcggcagat
    11221 gccgatgaac gccttgcggc cttcgacgtg catcaagccg gcccccgcgg cccaggtctc
    11281 ctccagcagc agccgcagga cgtcgctggg cgaggcatag ggcgcgaaca ggtcgcgcat
    11341 ccgctggatg ttggtgcgcg cctcggcgtg gtagcgatcg cgcgaagcgc tgtcactgat
    11401 ctcggagtag gcgaatccga tgcgcttgaa ctccgcggcc tgactcagac ttccccggtc
    11461 ggggtggtcg aagagctgcc gccgcgccga ggcgagggcc tccgggtgca cgaaccccgg
    11521 gacgtggacg gccagcgtgg tgccttcgac gaccgagagg atgtcctctt gggacaactg
    11581 gcatgacgtg gtgcgcttga cgttgatgat ggtcccccgt gtgtgcggcc cgcgtgcccg
    11641 gcagtgaggc gcgggggcgt tttgcaggta atgccatcat ctcggccgtt aattaccgga
    11701 tcctcatgct gtgtatcgat tcacaggtgc acccttggat gaggcagatg caccggccgt
    11761 tcctcccgac gacggagacg accgcgtggc gcctccgtcg tggggcaggg ggcgcacccc
    11821 ggatactcgc catgggcgcg cgcctgcgcg ttcacacccc gcccggcgat gcccggtgcg
    11881 ccccgggctg acgtcacaac cagctgacac gggcgtccgc gatgtcctcc tcggccaggc
    11941 cggcgggcgg acgggcgagc tcccccttgg ccacaccgtc gagcagcctc cgtaccgccg
    12001 ccatgatgtc ggggacggcg ttgcggaggt cggcggcgtc gcccggaccc gcctcggagg
    12061 ccgggacgtc ggcctccgtc aggagggtgt cgaggtccgc cagcctgccg gccacccagt
    12121 ccagcggtgc ggcggagagt tcctcgacga agacgcgcgc gcccggctcc cagccgtcga
    12181 acctcgggtg gtggtgcgcc cggtccaggc tcccggacgg gcccgccacg gactccagga
    12241 gatcggcccg ccacaccggg cggtcgacca cgatgggccg cgacgcgtag atgcctcccg
    12301 gcaggtctcc ggaccgcagg acgcggacct ccaggcgcac ccctcgttcg gcgccctcct
    12361 gccccgggac cgggtcggga tccacgaagt acatgtcgct cgccaccacg ccgatccgct
    12421 cgaaaccgca gacgtacagc atgtgccctc ccgaatgccc cgactgacgg aaccgctgtc
    12481 ctcagagtga gacgatgccc gcaggtacgc cagagggccc gatcgagcca cgggcgactg
    12541 cccggacagc ccccactcgc gccgccatcg gacgtcacgc gatcccgccc tcgccggacc
    12601 tgcgcccgcg ccggcgcggc acgctgatcg tcaacgtcgg ggacaaccac gccgcgacca
    12661 cccgcgaggc ggttttcgtc tcctactcat cgcgctgtcg gcgcaccatc tcggtgatcc
    12721 agacgggcgc gaacggagac gtgcagcccg gggaagtcgg gtagtccttg agtacctcca
    12781 agcgctcacc gatgtcgatt gcacgggtgc ggtgctcggc gtgctcgatc ccgatctggg
    12841 ccaggcagtg gttcatcgcc cactgcaggc gatccggggc gtctttcatc tccgcatcga
    12901 tgacgtcgag cagccccgcg aggtcgaggc cctcgggatg cttggtcacg cgttcggtgg
    12961 tcagcgccca gccggcgctc gcgaccactg gatccggatc ggcggaccag gccaggcgca
    13021 gctcttcgga gtgcgggttc ttcttcacca cgtagttcac gagccagtcg tgcaccttgg
    13081 gtgtgcgcgc ctcgcgcaac atgccgtcca gctcgtcacg ttcgaacgcc ttcgggcggc
    13141 agatcagggt cgccagcagc cttgccgcgg tgtcacccgt ctcccagagt tggcacgcga
    13201 gctcctgctg cgtcttcagc cgcttcgcga gcgcgcgcag cttgccgagg ttcacaccgt
    13261 gatcgtcacc gtgtttctcg ttcaccgcgc gggtcttcgg gtcatcgagc ccggccagct
    13321 cggccatcac ctcggccaac gccgtctcgg ccacctcagc ctcctgtcca tcacgtgcga
    13381 gattcagcct acgacggttc acgatcggcc tcgcccggtt caccacggcc ggccgtcgca
    13441 cccccgcacg cgtcgtaccg gtccatatcg cacaggtgtg cgtcacgggt catccggctg
    13501 cctgccacct cgtgcgctgc cggggccttg atgtgagcgt ggcagtacag cgtggagccc
    13561 gagaactgcg aggaggtgat cgtcgtggcg ggaccgaaca gggaggttcc gggcgaggac
    13621 atcgtcaagg gccggccgat acgtcctcgg cgagccgggg ggtccggacc ggaccgggcc
    13681 gggtcggccg cagagcgtcg acgtgtccgc cttcgtggac cttgcccggg ccgagcccgt
    13741 catggttctg caccacgaca cccgccgcga ccggctgatc aataccttga tgaagcagac
    13801 cggcaagcac aagacggcct gacctgcgca ccgcgggtct ccgccgcggg ccgcatctcc
    13861 gtccgcagcc ggatagatgg aatggtcata ccaatgcggt gaccgttgaa ttccccgctc
    13921 cctcccccca ccatggcctc ggaggcacct catgcccgag caccactccg gcccgggccg
    13981 cccgcatccc cccacagcgg cggccatcct cctctccctg ctgaccgtac tcaccctgct
    14041 cgtcactctc cccggtaccg ccgccgcggt gcccgggaac gacatccggc cgacccaccc
    14101 cgagcaggac tggatgggct cgaccatccg cgcccacgag ggcgaacgcg gcccggtcgc
    14161 caagggctcc tcactgcccg acccctcgct gcccgacctc cccctggcgg gccccccggc
    14221 gagcgcctcg gtgagcgccg gggtcaaggg cgtggacgtc agcagccaca acggcaacgt
    14281 ctcctggtcc aagctctgga gcagcggcat cagattcgcc tacgccaagg cgaccgaggg
    14341 caccagctac accaaccccc acttcaccca ccagtacaac ggcccgtacg acgcgggcat
    14401 gatccggggc gcgtaccact tcgctctgcc gggcaactcc agcggcgccg cacaggccga
    14461 ctacttcgtc gaccacggcg gcggctggac ggacgacggc tggactctgc cgggcgtgct
    14521 cgacatggag tacaacccct acggcagcac ctgctacggc ctgagcacgg gccggatggt
    14581 cgactggatc gaggacttcc tcgacgccta ccgggaccac accgggcgcg acgcggtgat
    14641 ctacacctcg accgcgtggt ggaagaagtg caccggcaac cacggcggat tcggcaagta
    14701 caacccgctg tgggtaccgc gctacggctc ttcctccggt gagctcccga acggctggga
    14761 cttctacacc ttctggcagc acacgaacac cggccccacc gtcggcgacc acgaccgctt
    14821 caacggctcg tacgacaggc tcgaggcgct cgcctacggc tgaccgggcc acggggtgaa
    14881 cgacggtggg ccctggctcc tcggcgccgg ggcccgccgt cgttcaccca ccagcagcca
    14941 gtactgcggt ctagcctcgt gcttcttgtc gggggtggag taccccagtt ggcgggaagc
    15001 ccggtgctgc acgtatatcg cgttgtgagc ccatgggcag gcgcataagg tcatcggcat
    15061 gtctgtacgt cttcgtatgc gtcaagcact gccggaagcg atgcgcgccc gtgacaaggc
    15121 cgcagtgagc gccctgcgcg cgacactggc cgcgctggac aacgccgagg cggtgcccgt
    15181 ggacgaagcg cagctccgcg gcgtggccct tgagcatgcg ccggtcggtg ccggcgccac
    15241 cgaagcaccg cggcgtgagc tgagcgagca cagcatggtg gatgtcgtgc gcgccgaggt
    15301 caccgaacgg ctggaggtcg cagcgcagtt gaccgcaccc gcgcatgccg accgagccgc
    15361 gcagctccgc gcggaggctg ccgtgctgct tcgcttcctc gacggccccc gcgccgcata
    15421 ggatccggcg ctgcatcacc tgcgtacgac gagcaggagc aggagcagga gcaggagcag
    15481 gagcagggca ggccgggaat gggtgcggtc ggcgtgcggt cgggcgcgct ccggggccgc
    15541 cccgagtttg agccggctct tgttacggcc cggcgtcggg ttgtgtgatc tcgaccaggc
    15601 gcggggcgga gaagcaccag cgcagccaca gcactgattc gatgtcccag aagggtgccg
    15661 cggccaggcc gtccgccacc gggatgcgga acccctgggc cgcgacgatc gcgcgcaggg
    15721 tcatgtgcac cccggcgaag tcccgggcgg ccttcgccca gtcggggtag acgtcgtcgc
    15781 cgcggaccag cgggtagcgc ccgacgaagg ccgcccagtc acatgcgccg ccgatgtcga
    15841 ggacgtcggc ggtgaggtcg gtgaccggca gctcccaggt ctgccagggc agggggtgga
    15901 gactcgatcc cagatcgaga tacatccgcc acatgcccgg cccgtcacgc cgcgccgtgg
    15961 acgtgtagag ccccaggtgg aacggcttcg tgctgggcgg accggaatcc ccgccgggct
    16021 cggtgaaccg ctcccggtcc ggggcgggcc gccggtgcgg catcagctcg tgcgggctgg
    16081 tgccgatcca gtgctgtcgt gtgcggtcga gcggcccccg gacgaggcac tcctggtccc
    16141 gggcgagccg ggccagcagc aggcagccgt cggccatctc gtccgcctcc tccacctcgg
    16201 acggggcaag gacggccgga ccgcccgcca gggccgtcac ttcgggttcg gtccaccccg
    16261 tcatgacgga ggtgaagtgg cgcaggccgg cgtccgtgcc ggcggtcggg tccgccgtgt
    16321 cgagctgtgt cactcgccgg cgaaggatca gggcaggggt gggacgcaca ccgtgatcct
    16381 tccccggtgg cgccccctgc aacacggcgc cgtccttccg gcggccgcgg gtcagcagga
    16441 gagggagacc tcctggccga gagcgttggc cgagtcggtc ggcgggttgt agccgggcgg
    16501 gaagacgacc ttgaagtaca tccagccccg gtatttgccg gggctgcagc tcacggccgc
    16561 gttgttctgg gcgctgcggg tgttcctctg tgtcctcggc gtcgactgct tgacgagatg
    16621 cccgttcttg tacagggccg cgcgggtggt gatctccggc accggtgccg agcacgagac
    16681 cgtcacgacg acgttgatgg tgccgggcac gtgggtggag ttgtgcgggc gctgcacctg
    16741 gccctggcat cggatcaccg cggacgcgtc ggcgtccgaa ccggccttgc cggcatccac
    16801 gctacccccg tcagcgtcgc gcccctccgg cacggtggcc ggagcggacg ggtcgccggc
    16861 cgccgcggcg ggggaggtgc cgccgaccaa cgccaggacc gccattgacg cggcggcgac
    16921 tgccgtcctc catgtgcgca tatctgctcc tccatgtgcg tctgctccgg tttgggtgtg
    16981 ggcacaccaa gaagagactg tacaaagggg aatcgattca tcatgaaagc tgacatgtgt
    17041 tcgagcgtgg atcccggtcg gttcgggcgc tgcattgtcg gccggactca gctggtgtga
    17101 gcaggatgcg ggtcggggag ggtgccggac ggccagagcg tctcggggaa ggcggcctcg
    17161 tcggagtcgg tgtcaggcat gagggtggtg agcatctgct cggcggtctt ggcgttgtcg
    17221 gcggcgcggg gcagcatgac gctctcgtag gcgtcgagtg ccgcgtcgat ggcggagtgg
    17281 tcgatgacgg ctcgggcgag ttcggcgccg tcgagcaggg cgaggttggc gccgacgccg
    17341 gcgggcggca tcaggtgggc ggcgtcgccg agcagggtga tgccgggcac gcgctgccag
    17401 gagtggggaa cgggaagggc gaacacgggc cggttgatga atccgccctc gttctcgcgc
    17461 aggatgtcca gcagactctc gtgccagccc tggtactggg cgaggaggcg cgcgcgtacg
    17521 gcttcggtgt ccgcgaggtc gaggccggcg tgccagtcct gggggccgcg gaaggtgacg
    17581 taggcgcgga cgtgtccgtt gctgttgcgc tgcagcacca ggctgcggcg gcccgccttg
    17641 gccgtcatgg tgccgcggcc gaccaggtgg gcgatgccgg ggtgacggtg gtcgacgtcg
    17701 tcgaagtggg cttccaccat cgtgacaccg gcgtaggagg gttgggcgtc ggagagcgcg
    17761 gagcgcactc gggaccaggc gccgtcggcg ccgatgacca ggtcgaactc ctcgacggtg
    17821 cggtcgtggt ggtgcaggcg agcggcgccg tgggggagcg gggtgacggc ccttaccgag
    17881 tggccccagc gcactgttcc gggggcgagg gagtccagca ggagcgcgcg tagctgaccg
    17941 cggtcgatct cgggcctgct cagatcgccc tcgccggcct ggtcgtggcg tatgagcgac
    18001 gcgtggcggt cgtacaggcg ccactcctgg ccttcgggcc gggacagggc gaggaactgg
    18061 tcgaacagac cggctgcgcg cagggctgcc tggccggtgt cttcgtcgat gtcgagagtg
    18121 ccgccctgtg agcgggagtg cgggtgggtc tcgccctcgt agacggtgac cggcacgccg
    18181 tgctgctgca ggacgcgtgc gcagacgagg ccgccgaggc cggcgccggc gatcgcgatg
    18241 cgaggggtgg tgaacatgag ggtcacgctc cgttcgacgg ttgaggagag tgctggtgtc
    18301 ttgcggtgac gccctgttcc gggcagggcg gatcagccgt tccggcgtgg gggcgcgcca
    18361 cggatttccg gcagctgagt gccagtcaga tgtacctgat cacagaaaca cagccgccct
    18421 aaaagtacaa ctgctacgac tttctatccg attgcctttg cgatcgaatc ccctcaagcg
    18481 ggtagggtgg gcgcatgact ggcacagcga caggacgccg cgagcgcaag aaggcccaaa
    18541 ccagacaggc cctggcggat gccgcggtgc ggctgttcac cgagcgcggc ttcgacgacg
    18601 tcggtgtgcg cgaggtggcg gaagcggccg acgtctcgct gagcacgctc ttcaagcact
    18661 tccccagcaa ggaagccctc gtcttcgacc tggacgcgga cgtcgagagt gccctggtcg
    18721 ccgccgttag tgaccgggcc cccggtcagt ccgtgctgca cgccctgcgc gaccacatgg
    18781 tgcacgcccg taccgctgtg cggaccgacg accccacgta cgtcctcgtc gaatccactc
    18841 ccgcgctgcg ggactacgcc cggcgcatgt ggtcgcgcca cgagaagacg ctggctgcca
    18901 ccctcgccga ggccaccggg ctcaccccgg aggaccccgc cgtcgccggc ctggcacgct
    18961 tcaccctgga agcccccagc atcgcccgtg cgagcgaaga cccgccccgg accatgcgcg
    19021 acttgttcaa cctgctggaa cacggctggg ccaccaccct cttggcacga cagggggagg
    19081 gctgccccgg tcgggacggg tgacggcgat cggatgctgc agcagtgcag gcaggctgac
    19141 ccaccgtcaa gaatgcgagc cgcgtcaggc gtccatcttc tgagcgtgtt gcagaggatt
    19201 ctctgcagag ggccctatgc agagagtcct ctgcaactta cgatgtcggc atggccgatg
    19261 aacccgagcg cacatcgcaa cccgccaagc ccacacgccg cctcgatgcg cgcagcctgc
    19321 gcgggctggc ccatccactg cggatgcgga tcttcgaact gctgagtctg gacggccccg
    19381 ccaccgccac ggggctctcc gagcgtctcg gggagaacac cgggaccgtc agctggcatc
    19441 ttcgccatct cgcagagcac ggcttcatcg aggaagaggc cgaacgggga acgaagaggg
    19501 agcgctggtg gagaagggcc ggcggcacaa acgagctgca caccaccgat ttccgtgacg
    19561 atcccgacag ccgtggtgcg ctgtcggtct acttgcacga actggtgcac cagtacttcg
    19621 gcagggtgac gaactacctc aacgaggact gggatgacgc atggcggggc gccggcaccg
    19681 tctcggactg gcgcgatcta cggatgacgc cggatcagct ggcggcgctc aacgcggacc
    19741 tgatggcggt catcgcccgc cacacgcccg ccccggacac cgaaccggac cccgacgcgc
    19801 tgcccgtcgt cgtgcagctc cagtcgttcc cccgtaagaa acggggcacc gcatgacccg
    19861 gggcacacac cgcggcggcc tcctgcggcg ccaccgggac ttccgcctgc tgtggtgcgg
    19921 cgagaccgcc ggcaagttcg gcgcctccgt caccggggtg gcgatgccgc tgatcggcgt
    19981 aaccgagctg cacgccggca cgttcacggt cggcctgctg accgcggcca cctggagccc
    20041 ttggctgatc atcggcctcc ccgtgggcgc ctgggtggac cggaaccgac gcaggccgat
    20101 catgctggcc gccgccgcgg tctccttcgt cctgttcgcc ggcgtccccg tcgctgcttg
    20161 ggccggtcag ttgagcacgg gtctgctgct ggcggtcgcg ctcctgacgg gcacggccgc
    20221 agtgttcttc cagaccgctt acagcgccta cctccccagc attctggaac ccgacgacca
    20281 gcccgaaggc aacgccaagc tgcacggcag cgcagccgcc gcacagatcg ccgggctcag
    20341 ctcgggcggc ctggtcgcgc agttcgccgg tgcggtcaac gcgatgttcg ccaacgccac
    20401 cacgtttctc gtatccctcc tctgcctgtc gggcatccac caccgcgagc cgcgccccac
    20461 cagaacctct cgtcgctcca aaactctggt cggtgacatc ggtgagggcc tgcggctggt
    20521 cgcaggagac ccctggctcc gcaccctgac gctcttcggc gccacctcca acctggccct
    20581 catgggatac caatcgatcc tggtggtctt cctggtccga accgtcggac tggaccccgg
    20641 ccccgtcggc gcaatcatcg cggccgccgg tgcagggggc gtcgccggag ccctcgtcgc
    20701 acgcagggtc gccgcccggg tcggcacggc ccgcgcgacg ctgctcttcg aactgggact
    20761 gcccgtattc gccctgctca tcccactgac cacggacggg gccggagccc tgctcttcat
    20821 agccg
//
