LOCUS       NZ_CP013141            10876 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Lysobacter antibioticus strain ATCC 29479 chromosome, complete
            genome.
ACCESSION   NZ_CP013141
VERSION     NZ_CP013141.1
KEYWORDS    RefSeq.
SOURCE      Lysobacter antibioticus
  ORGANISM  Lysobacter antibioticus
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Lysobacter.
REFERENCE   1  (bases 1 to 5765176)
  AUTHORS   Kobayashi,D.Y. and Ravel,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2015) Plant Biology & Pathology, Rutgers, The
            State University of New Jersey, 59 Dudley Road, New Brunswick, NJ
            08901, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: TiGR Assembler v. 2
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Sanger; 454 FLX
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001442535.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 11:28:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,784
            CDSs (total)                      :: 4,717
            Genes (coding)                    :: 4,635
            CDSs (with protein)               :: 4,635
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 12 of 82
            Pseudo Genes (incomplete)         :: 74 of 82
            Pseudo Genes (internal stop)      :: 2 of 82
            Pseudo Genes (multiple problems)  :: 6 of 82
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:11:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1644519
            Orig. end                         :: 1655395
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP013141.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10876
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10876
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10876
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5876](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5876
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(477..488)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            881..1786
                     /gene="pstA"
                     /locus_tag="GLA29479_RS06775"
                     /old_locus_tag="GLA29479_1544"
     CDS             881..1786
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155848.1"
                     /locus_tag="GLA29479_RS06775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1544"
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="WP_057917512.1"
                     /transl_table=11
                     /translation="MNATPAPIDAELQRRHRVADSLYGRRRVFNAIAVIMACAAAAFGL
                     FFLGWILYTLLAKGLGGINLALFTQNTPPPMQEGGLLNAFFGSAVMCLIAIVLGTPLGI
                     AAGTWLAEYGAGRKIGTVVRFVNDILLSAPSIVLGLFVYTLVVMQSGGNFSALAGAISL
                     AFIVLPVVVRTTDEMLRLVPSQMREAALSLGVPQWKVIVQVLYRSASAGIVTGVLLALA
                     RISGETAPLLFTAFGNQYWSHNVLQPMASVPVVMNQFAGSPYETWQTLAWAGALVLTFF
                     VLVVSLLARALVLRNRISND"
     gene            1779..2636
                     /gene="pstB"
                     /locus_tag="GLA29479_RS06780"
                     /old_locus_tag="GLA29479_1545"
     CDS             1779..2636
                     /EC_number="7.3.2.1"
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstB"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237787.1"
                     /locus_tag="GLA29479_RS06780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1545"
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="WP_082638354.1"
                     /transl_table=11
                     /translation="MTDLSNQPVARTPAMNDARLSVATPDRAAAVVGASPVKLAARGLD
                     FYYDKFHALKSINLEIPEKQVTALIGPSGCGKSTLLRIFNRIYALYPKLEARGEVLLDG
                     ENILDPKYPMNRLRSKVGMVFQKPVPFPMTIFENVAYGIRHHEKLSKSEMTDRVEQALR
                     QGALWDEVKDKLAQSALGLSGGQQQRLCIARAVALKPSVLLLDEPTSALDPISTSRIEQ
                     LVEELKKDYTIAIVTHNMQQAARVSDFTAFMYLGDLIEHGPTEQIFSKPTKQQTEDYIT
                     GRFG"
     gene            2785..3495
                     /gene="phoU"
                     /locus_tag="GLA29479_RS06785"
                     /old_locus_tag="GLA29479_1546"
     CDS             2785..3495
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="phoU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994733.1"
                     /locus_tag="GLA29479_RS06785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1546"
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="WP_057917510.1"
                     /transl_table=11
                     /translation="MSTQMHDHIVKSYDDEQRRLLDETLRMGEMAASQLEAALDVVQRR
                     DDKAAERIIANDEAIDALEQEISHDVMKLALRGPMARDLREILAAIRIASDIERVGDYA
                     ANVAKRSTALNLSPPLPHVAGLHALGTLAVKQLRDVLTAYRDNDIELAQSVRARDAEVD
                     TAYTGLFRELLTYMMEDARSITACTHLLFMAKNIERIGDHATNIAENVWFLVKGEEQLP
                     PREKRDDTNTTGAV"
     misc_feature    complement(2801..2816)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.64"
                     /tool="antismash"
     misc_feature    3652..3663
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.38"
                     /tool="antismash"
     gene            3807..4322
                     /locus_tag="GLA29479_RS06790"
                     /old_locus_tag="GLA29479_1547"
     CDS             3807..4322
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436357.1"
                     /locus_tag="GLA29479_RS06790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1547"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_057917509.1"
                     /transl_table=11
                     /translation="MTLLMANGDLAAARSGDRAALERVLTHSRQDLRRYAEYHCVINDI
                     EDAVQESLITVSRKLRDLRVLECFVSWTFRIVKRECNRLKRGRRMLSGDVITDEILPVV
                     TPEPCEWRHDVAAALESLPAHYREIVLLRDLEGLTIAEIGDQLGLSREAVKSRLHRARI
                     LAREYLAP"
     misc_feature    complement(4373..4390)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.59"
                     /tool="antismash"
     gene            4484..4987
                     /locus_tag="GLA29479_RS06795"
                     /old_locus_tag="GLA29479_1548"
     CDS             4484..4987
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483445.1"
                     /locus_tag="GLA29479_RS06795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1548"
                     /product="hypothetical protein"
                     /protein_id="WP_082638355.1"
                     /transl_table=11
                     /translation="MSKQIKKPIALAIGATLLGGLSLSASAFAMSDLSQGYLLGAQQAP
                     PAAEKAADAKAAADTAKHAEGTCGADKKAKEGSCGGDKKHAEGKCGEGKCGADKKKAGE
                     GKCGEGKCGADKKKAGEGKCGEGKCGGDAKAAAAAPAATAPTATKAKKAAEGKCGEGKC
                     GGSV"
     gene            5001..5876
                     /locus_tag="GLA29479_RS06800"
                     /old_locus_tag="GLA29479_1549"
     CDS             5001..5876
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534610.1"
                     /locus_tag="GLA29479_RS06800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1549"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_211265037.1"
                     /sec_met_domain="DUF692 (E-value: 1.5e-112, bitscore:
                     373.3, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMMDRSRPLKPDAVGLGLRRALLGPLQSAAAGDFDFLECAPENWI
                     GIGGRYREALRDLASRHPLVCHGLSLSLGGTAPLDETFLIRVRRFLDEHRVACYSEHLS
                     YCSDDGHLYDLLPIPFTEEAVHHVAARIRRTQDIVGRRIAVENASYYAAPHQEMSEIEF
                     TRAVLAEADCELLLDVNNIYVNSINHRYDPREFLAAMPGERIAYLHVAGHYDEAEDLKV
                     DTHGAPVKHEVWSLLGDAYRLFGARPTLLERDFNFPPYEELVGELDIVRRVLGEAAPQA
                     GAASRAQAAR"
     misc_feature    5033..5047
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.42"
                     /tool="antismash"
     gene            5873..6667
                     /locus_tag="GLA29479_RS06805"
                     /old_locus_tag="GLA29479_1550"
     CDS             5873..6667
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412767.1"
                     /locus_tag="GLA29479_RS06805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1550"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_248842808.1"
                     /transl_table=11
                     /translation="MSDNASMSDAPSRLRAQQFELTRHLRDPQASPAPAGIEDRRLGIY
                     RDLLFNNIEGLLASNFPVIRKLLGEQRWTALVRDFYRDHRCHTPLFAEIAREFLRYLPE
                     RDADDPPFLSELAHYEWVELALQLSEAQAPQDDAGIGDADLLEHAPELSPLAWPLAYAW
                     PVHRLGPEHQPEQPSPTPTLLLLRREADGEVRFSELSPLAFRLIERIGETPGLSAREQL
                     RALAQEAGSDDVAAFLELGHGLLRQMRASGVIFFAAPANPFA"
     gene            6729..7454
                     /locus_tag="GLA29479_RS06810"
                     /old_locus_tag="GLA29479_1551"
     CDS             6729..7454
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336970.1"
                     /locus_tag="GLA29479_RS06810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1551"
                     /product="DoxX family protein"
                     /protein_id="WP_082638357.1"
                     /transl_table=11
                     /translation="MARPRIATCLALRDRLAGVGAWLSPLGLRLLLAWEFYESGREKLH
                     GENWFGNVMANFPFPFDRVPADLSWGMATWFELLGGVALLIGLGTRLVAASLFVLTVVA
                     IAAVHWPQDWMGLSQLVQGYAITDQGAGNYKLPLLFLAMLWPLILGGAGRLSLDALLAR
                     AAALCAPAPRDDLTGWGLVLLPVGVVVAMLLPAPGLALAGLGAALLLAATARSRRTRGA
                     GAASQGDFLRSPASTAQSR"
     gene            7591..8241
                     /gene="rnt"
                     /locus_tag="GLA29479_RS06815"
                     /old_locus_tag="GLA29479_1552"
     CDS             7591..8241
                     /GO_function="GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /GO_process="GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rnt"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598436.1"
                     /locus_tag="GLA29479_RS06815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1552"
                     /product="ribonuclease T"
                     /protein_id="WP_057917507.1"
                     /transl_table=11
                     /translation="MIETGPTPSIAPAAPTLANRFRGYLPVVVDVETGGFDWNKHALLE
                     IAVQPLELDADGRLVPGPMSSAHVVPAPGTLIDPKSLEVTGIDIDHPFRDAKPERQALE
                     SVFAPVREALKRQGCQRAILVGHNAHFDLNFLNAAVARSGHKRNPFHPFSVFDTVTLAG
                     VAYGQTVLARALQAAELEWNAQDAHSAVYDTERTAQLFCKIVNAWPQRGISAP"
     gene            8397..9290
                     /locus_tag="GLA29479_RS06820"
                     /old_locus_tag="GLA29479_1554"
     CDS             8397..9290
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GLA29479_RS06820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GLA29479_1554"
                     /product="hypothetical protein"
                     /protein_id="WP_057971151.1"
                     /transl_table=11
                     /translation="MGEAWFMGDERHMYTALMHEDPQQWPRTELDSALYELSSGPVSFG
                     PMREWILWFAFLLPRALELVGRFRADAFIDRQLYAALVTATFVHCPDPEQLPAGVRRDL
                     LDTLGRAMFAPSHWSACGLATNAFFSPVQRLPGLHPSDSLYPAGAFSASCTLIAKYLEP
                     EFIEGWLASALAIDDPYWRAGWVAWLAGAAPMLLDGAYPDALPLDREHPTAWSNSHLVQ
                     APSALVVAGSAPASFLSAESRHALTAALRKQLSHEQLQRWGARLTEAGAQRPDFEYIFL
                     KYRSAAQCVLNRYRLD"
     misc_feature    9144..9146
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9456..10067
                     /locus_tag="GLA29479_RS06825"
                     /old_locus_tag="GLA29479_1555"
     CDS             9456..10067
                     /GO_function="GO:0004568 - chitinase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006032 - chitin catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012409.4"
                     /locus_tag="GLA29479_RS06825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1555"
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="WP_057917506.1"
                     /transl_table=11
                     /translation="MFAFDDEQFLDGYRRRFGPLRDELAQAVRFLIGRIEQDASFNQNP
                     VHRHQIAYCLATFKWETAHTLRPIDEFGDDARFERLYGPNTRVGKMLGNTRAGDGARFH
                     GRGYVQLTGRANYERAGKILGIDLINHPDEAKKPEVAYAIAMEGMKKGWFTGKKLDSYF
                     KPGQLPNYEDARRIINGQDKAQTIADIARRFDELLVNSLH"
     misc_feature    complement(9457..9472)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.79"
                     /tool="antismash"
     gene            complement(10194..10505)
                     /gene="sugE"
                     /locus_tag="GLA29479_RS06830"
                     /old_locus_tag="GLA29479_1556"
     CDS             complement(10194..10505)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="sugE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451011.1"
                     /locus_tag="GLA29479_RS06830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GLA29479_1556"
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="WP_057917505.1"
                     /transl_table=11
                     /translation="MPWILLFFAGLFEVGWAIGLKYTEGFTRLWPSVGTIAAMAISLGL
                     LGVAMKSLPVGTAYAVWVGVGAVGTVILGIVLFNEPLNTLRVASVLLIVAGLVGLKLA"
     misc_feature    complement(10468..10483)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.03"
                     /tool="antismash"
     misc_feature    10572..10585
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.4"
                     /tool="antismash"
ORIGIN
        1 ccgccaccgt catcttcgtc ctgttcgctc tcgccagcgc cgccttgtcg atgctctggg
       61 gcggccgcca cgtgctggcc aaggaaggtc tcgatttctt catcaccgcc gaatggaacc
      121 cggtcgagga ccaatacggc gcgctcgtcc cgatctacgg caccatcgtc accgcactga
      181 tcgcgatggt gatcgcggta ccggtgagct tcggcatcgc cttcttcctg accgaagtcg
      241 caccgcgctg ggcgcgcggc ccgatcggca ccgccatcga actgctcgcc ggcattccct
      301 cgatcatcta cggcatgtgg ggcctgttcg tgctggtgcc ggtgatgacc gaacacgtca
      361 cgccctggct caacgaccac gtcggcacct ggccggtgat cggcaagctg ttccagggcc
      421 cgccgctcgg catcggcatg ctcaccgccg gcatcgtcct ggcgatcatg gtgatcccgt
      481 tcatttcctc ggtgatgcgc gaagtgttcc tgaccgtgcc gacccggctc aaggaatcgg
      541 cctacgcgct gggctcgacc aagtgggaag tcagctggga catcgtcctg ccctatacgc
      601 gctcggcggt gatcggcggc gtgttcctcg gtctcggccg cgccctcggc gaaaccatgg
      661 cggtggcctt cgtgatcggc aactcggtca acttctcggc ctcgctgctc gagccgggca
      721 cgaccatcgc cgcgctgatc gccaacgact tcggcgaagc caccgagacc tatcgttcgg
      781 cgctgctgct gctcggcttc gtgctgttca tcgtgacctt cttcgtcctc gccgccgcgc
      841 ggctgatgct gatgcaactc tcgcgcaagg agggcaactg atgaacgcga ctcccgctcc
      901 catcgacgcc gaactgcagc gccgccaccg cgtcgccgat tctctgtacg gccgccgccg
      961 cgtgttcaat gcgatcgccg taatcatggc ctgcgccgcc gccgcgttcg gcctgttctt
     1021 cctcggctgg attctctaca ccctgctcgc caagggcctg ggcggcatca acctcgcgct
     1081 gttcacccag aacacgccgc cgccgatgca ggaaggcggc ttgctcaacg ccttcttcgg
     1141 cagcgcggtg atgtgcctga tcgcgatcgt gctgggcacc ccgctcggca tcgccgccgg
     1201 cacctggctg gccgagtacg gcgcaggccg caagatcggc accgtggtgc gcttcgtcaa
     1261 cgacatcctg ttgtcggcgc cgtcgatcgt gctgggcctg ttcgtctaca ccctggtggt
     1321 gatgcagagc ggcggcaatt tctcggcctt ggccggtgcg atctcgctgg ccttcatcgt
     1381 gctgccggtg gtggtgcgca ccaccgacga aatgctgcgc ctggtgcctt cgcagatgcg
     1441 cgaggccgca ctctcgctcg gcgtgccgca atggaaggtg atcgtgcagg tgctgtaccg
     1501 cagcgcctcg gccggcatcg tcaccggcgt cctgctcgcc ctggcgcgca tctccggcga
     1561 aaccgcgcct ctgctgttca ccgccttcgg caaccagtac tggagccaca acgtgctcca
     1621 gccgatggcc agcgtgccgg tggtgatgaa ccagttcgcc ggcagccctt acgaaacctg
     1681 gcagaccctc gcctgggccg gcgccctggt gctcaccttc ttcgtcctgg tcgtcagcct
     1741 gctcgcccgc gccctcgtgt tgcgcaatcg gatctccaat gactgatctc tcgaaccaac
     1801 ccgttgcccg gaccccggcc atgaacgatg cacgcctttc cgtcgccacc ccggaccgcg
     1861 ccgcggccgt agtcggcgct tcgccggtca agctcgccgc gcgcggcctg gacttctact
     1921 acgacaagtt ccatgccctc aagtcgatca acctggagat ccccgagaag caggtcaccg
     1981 cgctgatcgg cccctcgggt tgcggcaagt cgaccctgct gcgcatcttc aaccgcatct
     2041 acgcgctgta tccgaagttg gaagcgcgcg gcgaagtgct gctcgacggc gagaacatcc
     2101 tcgacccgaa gtacccgatg aaccgcctgc gcagcaaggt cggcatggtg ttccagaagc
     2161 cggtgccgtt cccgatgacg atcttcgaga acgtggccta cggcatccgc caccacgaga
     2221 agctgtcgaa gtcggaaatg accgatcgcg tcgagcaggc cctgcgccag ggcgcgctgt
     2281 gggacgaagt caaggacaag ctggcccaaa gcgccctggg cctgtccggc ggccagcagc
     2341 agcgtctgtg catcgcccgc gcggtggcgt tgaagccgtc ggtgctgctg ctcgacgaac
     2401 cgacctcggc gctggacccg atctcgacca gccgcatcga gcagctcgtc gaagagctca
     2461 agaaggacta cacgatcgcg atcgtgaccc acaacatgca gcaggccgcg cgcgtgtccg
     2521 atttcaccgc cttcatgtac ctgggcgacc tgatcgagca cggcccgacc gaacagatct
     2581 tctccaagcc gaccaagcag cagaccgagg actacattac gggccgcttc ggttgaagca
     2641 gtcgggaatc gggaatgggg aatcgggcat cgccgaaagc gagagccggt tccgacgtta
     2701 cccgcgcccg tccgagctgt accgatgttg ccgttgtctt taccggttcc cgatttccca
     2761 ttcccgattc ccggcccaac gcccatgagc acccaaatgc acgaccacat cgtcaagagc
     2821 tacgacgacg agcagcgccg cttgctcgac gaaaccctgc gcatgggcga gatggccgcg
     2881 tcccagctcg aagccgcgct ggacgtggtc cagcgccgcg acgacaaggc cgccgaacgc
     2941 atcatcgcca acgacgaggc gatcgacgcg ctcgaacagg aaatcagcca cgacgtcatg
     3001 aagctggccc tgcgcgggcc gatggcgcgc gacctgcgcg agatcctggc ggcgatccgc
     3061 atcgcctcgg acatcgagcg cgtcggcgac tacgccgcca acgtcgccaa gcgttcgacc
     3121 gcgctgaacc tgtcgccgcc gctgccgcac gtggccggcc tgcacgcgct gggcaccctc
     3181 gcggtcaagc aactgcgcga cgtgctgacg gcctatcgcg acaacgacat cgaactggcg
     3241 cagagcgtgc gcgcccgcga cgccgaggtc gacaccgcct acaccggcct gttccgcgaa
     3301 ctgctgacct acatgatgga agacgcacgc agcatcaccg cctgcaccca tctgttgttc
     3361 atggccaaga acatcgagcg catcggcgat cacgccacca acatcgccga gaacgtctgg
     3421 ttcctggtca agggcgaaga gcagttgccg ccgcgcgaga agcgcgacga caccaacacc
     3481 accggcgcgg tctgacctcg ccccgcccgc tccggcacgg tccggggcgg gattcgcgca
     3541 gacgcgtcgc gctgcgttgc agcaaagccc ccaaccaggg cgacgcgacc gaccgatgag
     3601 cgggtcgaat cttgcgcccg tattgccgcc accggctggc ctgctcgccc tgcaaacggt
     3661 tacagccgtt ccagactgaa tccgctctaa acgaatccgc caacgccgtc gcaatcgcga
     3721 accgatcggc ccctctccaa actcttggta catgcccgta gcgccctgct gcggtgtatt
     3781 ttgcctacct cggaggagac ccgggcatga ccttgctgat ggcgaacggg gatttggcgg
     3841 ccgcccgctc gggcgaccgc gccgcgctgg agcgcgtgct gacgcactcg cgccaggatc
     3901 tgcgccgcta cgccgagtac cactgcgtca tcaacgacat cgaggacgcg gtccaggaaa
     3961 gcctgatcac cgtctcgcgc aagctgcgcg acctgcgcgt gctcgaatgc ttcgtctctt
     4021 ggaccttccg catcgtcaag cgcgagtgca accgcctcaa gcgcggtcgg cgtatgctca
     4081 gcggcgatgt catcaccgat gaaatcctgc cggtggtcac ccccgagccc tgcgaatggc
     4141 gccacgatgt cgccgccgca ctcgaatccc tgcctgcgca ctaccgcgaa atcgtcctgt
     4201 tgcgcgacct ggagggcctg accatcgccg agatcggcga tcaactcgga ctcagccgcg
     4261 aagcggtgaa atcgcgcctg caccgggccc gcatcctggc ccgcgagtat ctcgctccgt
     4321 gatgccccct tgcctcgctt acacccgtat cgctacgcag cccccggatc cgtaaccgct
     4381 cacttcgata gctttgcaca ccgcatccag acggaacccg gcacactggg ccgttcatcc
     4441 cgacggcggg cctcccgccg tcatcgccac gtaggagtca tccatgtcca agcaaatcaa
     4501 gaagcccatc gccctcgcta tcggcgccac cctgctcggc ggcttgagcc tgtcggcttc
     4561 cgcgttcgcc atgagcgatc tctcgcaggg ctacctgctc ggcgcccagc aggctccgcc
     4621 ggccgccgag aaggccgccg acgccaaggc cgccgccgat accgccaagc acgccgaagg
     4681 cacctgcggc gccgacaaga aggcgaagga aggcagctgc ggcggcgaca agaagcacgc
     4741 cgaaggcaag tgtggcgaag gcaagtgcgg cgccgacaag aagaaggccg gcgaaggcaa
     4801 gtgtggtgaa ggcaagtgcg gcgccgacaa gaagaaggcc ggcgaaggca agtgcggcga
     4861 gggcaagtgc ggtggcgacg cgaaggccgc tgccgccgcc ccggccgcga ccgctcccac
     4921 cgccaccaag gccaagaagg ccgcggaagg caagtgcggc gaaggcaagt gcggcggttc
     4981 ggtctgatcg caaccggcac atgatgatgg accgctcgcg tcccctgaaa ccggatgccg
     5041 tcggcctcgg cctgcggcgg gccctgctgg gcccgctgca gtccgctgcg gccggagatt
     5101 tcgacttcct cgaatgcgcg ccggagaact ggatcggcat cggcggccgt taccgcgaag
     5161 ccctgcgcga tctggcttcg cgccatccgc tggtctgcca cggcctgtcg ctgtcgctgg
     5221 gcggcaccgc gccgctcgac gagaccttcc tgatccgggt acgccgtttt ctcgacgaac
     5281 accgcgtcgc ctgctacagc gaacacctca gctattgcag cgacgacggc catctctacg
     5341 acctgctgcc gattcctttc accgaggaag ccgtgcacca cgtcgccgcg cgcatccgtc
     5401 gtacccagga catcgtcggc cgccgcatcg cggtcgagaa cgcctcctac tacgccgcgc
     5461 cgcaccagga aatgagcgag atcgagttca cccgcgcggt actcgccgaa gccgattgcg
     5521 agctgctgct cgacgtcaac aacatctacg tcaattcgat caaccaccgc tacgacccgc
     5581 gcgaatttct cgccgctatg ccgggcgaac gcatcgccta cctgcacgtg gccggccatt
     5641 acgacgaggc cgaagacctc aaggtcgaca cccacggcgc cccggtcaag cacgaggtct
     5701 ggtcgctgct cggcgacgct taccgtttgt tcggcgcgcg cccgaccctg ctcgaacgcg
     5761 acttcaattt cccgccgtac gaagaactcg tcggcgagct cgacatcgtc cgccgggtgc
     5821 tgggcgaggc cgcaccgcag gccggcgccg cgtcgcgcgc ccaagccgca cgatgagcga
     5881 caacgcaagc atgagcgacg ccccttcgcg actgcgcgcg cagcagttcg agctgacccg
     5941 ccacctgcgc gacccgcagg ccagcccggc gcccgccggc atcgaagacc gccgtctcgg
     6001 gatctatcgc gacctgctgt tcaacaatat cgaaggcctg ttggccagca atttcccggt
     6061 catccgcaaa ttgctcggcg aacaacgctg gaccgcgctg gtgcgcgatt tctaccgcga
     6121 ccaccgctgc cacaccccgc tgttcgccga gatcgcccgc gaattcctgc gttacctgcc
     6181 cgaacgcgac gccgacgatc cgccgttcct gtccgagctg gcgcattacg aatgggtcga
     6241 actggcgctg caactcagcg aagcgcaggc tccgcaggac gacgccggca tcggcgacgc
     6301 cgacctgctc gaacatgctc ctgagctgtc gccgctggcc tggccgctgg cctatgcctg
     6361 gccggtgcac cgcctcggcc cggagcatca gcccgagcag ccgtcgccga ccccgactct
     6421 gttgctgctg cgccgcgagg ccgacggcga ggtgcgtttc tccgagttga gcccgctcgc
     6481 cttccgcctg atcgaacgca tcggcgaaac gccggggctg agcgcgcgcg aacaactgcg
     6541 ggcgctggcg caggaagccg gcagcgacga cgtcgccgcc tttctcgaat tgggccacgg
     6601 cctgctacgg cagatgcgcg ccagcggcgt gatctttttt gctgcgccgg cgaatccttt
     6661 cgcctgactg ccgcctcttg tagtggaaag cgccggccaa tcggcgcagg tccccactgg
     6721 agcagctcat ggcccgtccc cgtatcgcaa cctgcctcgc cctgcgcgat cgtctcgccg
     6781 gtgtcggcgc ctggctctcg ccgctcggcc tgcgcctgct gctggcctgg gagttctacg
     6841 aatccgggcg cgaaaaactg cacggcgaaa actggttcgg caacgtcatg gcgaactttc
     6901 cgtttccgtt cgaccgcgtg cccgccgacc tgagctgggg catggcgacc tggttcgaac
     6961 tgctcggcgg cgtggcactg ctgatcggcc tgggcacacg cttggtcgct gcttcgctgt
     7021 tcgtgctgac cgtggtcgcc atcgccgcgg tgcactggcc gcaggactgg atgggcctgt
     7081 cccagctcgt ccagggctac gccatcaccg accagggcgc cggcaactac aaattgccgc
     7141 tgctgttcct ggcgatgctg tggccgctga tcctcggcgg cgccggccgc ctcagcctcg
     7201 atgccctgct cgcgcgtgcg gcggccctgt gtgcgccggc gccgcgcgac gacctgaccg
     7261 gctggggcct ggtcctgctg ccggtcggtg tggtcgtggc gatgctgctg ccggcgccgg
     7321 gcctggccct ggccgggctc ggtgccgcgt tgttgctggc cgcgacggcc cgctcgcgcc
     7381 gaacccgagg ggcaggagcg gcgtcccaag gggatttcct acgctcaccg gcctcgaccg
     7441 cgcaatcgcg atgacgacgc tccgggattg cgcggtcgcg actcgtgacc gcgcagttcc
     7501 tctcggctcg gccgccgctg ccggccccgc gaagccccgc gttgaccgcg ccggaacgac
     7561 cgattcgcga caacctgcta ggctttcgcg atgatcgaaa ccggccccac cccgtccatc
     7621 gcccccgcgg cccccactct cgccaaccgt ttccgcggct acctgccggt cgtcgtcgac
     7681 gtcgaaaccg gcggcttcga ttggaacaag cacgccctgc tcgaaatcgc ggtgcagccg
     7741 ctcgaactcg acgccgacgg ccgcctggtc ccgggcccga tgagcagcgc ccatgtggtg
     7801 cccgcacccg gtaccttgat cgacccgaag tcgctcgaag tgaccggcat cgacatcgac
     7861 catccgtttc gcgacgccaa gcccgagcgc caagccctgg agagcgtgtt tgcaccggtg
     7921 cgcgaagcgc tcaagcgcca aggctgccag cgcgcaatcc tggtcggcca caacgcccac
     7981 ttcgacctga acttcctcaa cgccgccgtc gcccgcagcg gccacaaacg caaccccttc
     8041 catcccttca gtgtgttcga caccgtcacc ctcgccggcg tcgcctacgg ccagaccgtg
     8101 ctcgcgcgcg cgctgcaagc cgccgagctg gaatggaacg cacaggacgc gcattcggcc
     8161 gtctacgaca ccgagcgcac cgcgcagctg ttctgcaaga tcgtcaacgc ctggccgcag
     8221 cgcggcatca gcgctccctg agcggtccgg atccggaccg ctgcgcatcg acaaggagtc
     8281 ggctcgtgcg cgcatcgcca agtaattcat cggcctatgt ctggctgcct ggccaaggcc
     8341 ccagtgcagg cgcgttacag cgtctgcgtg aacgcgcgcc cttgcctgag caagcgatgg
     8401 gcgaagcctg gttcatgggc gacgagcggc acatgtacac cgcgctgatg cacgaagatc
     8461 cgcaacagtg gccgcgcacg gaactggatt cggcgctgta cgaactgagc tcgggccccg
     8521 tttcattcgg accgatgcgc gaatggatct tgtggttcgc gttcctgttg ccgcgcgcac
     8581 tcgaattggt cgggcgattc cgggccgatg cctttatcga ccggcaactc tacgcggcgc
     8641 tcgttaccgc gacgttcgtg cactgtcccg acccggagca gctgccggcc ggcgtgcgcc
     8701 gcgacctgct cgacacgctg ggccgcgcga tgttcgcgcc ttcgcactgg agcgcctgcg
     8761 gattggcgac gaacgcgttc ttctcgccgg tgcaacgctt gcccggcctc caccccagcg
     8821 attcgcttta tcccgccggt gcgttctcgg cctcgtgcac gctgattgcg aagtacctgg
     8881 aacccgagtt catcgagggc tggctggcgt ctgccctggc catcgacgat ccgtattggc
     8941 gcgcgggctg ggtggcctgg cttgctggcg cggcgccgat gctgctcgac ggcgcctatc
     9001 cggatgcgct gccgctcgac cgcgaacatc cgactgcctg gtcaaacagc catctcgtgc
     9061 aagcaccgtc tgcattggtc gtggccggct ctgcgcctgc ttcattcctc agcgccgaat
     9121 cccgtcatgc cctgacagcg gcattacgca agcaactcag ccacgaacag ctgcaacgat
     9181 ggggcgcccg cttgaccgaa gccggcgcgc aacgccccga cttcgaatac attttcctca
     9241 aataccgcag cgcagcccaa tgcgtcctca accggtatcg actggactga actcgctctt
     9301 gccgacgatg ttgcaagcac gcaacacctc ggaattggcc gcccggcgcc gaacgccgcg
     9361 ttgaatggac tgcgagcggc acggcgccgc cacgaccggc aggacgcgcg gccgctttcg
     9421 cgcgcacatg ccgttgcgca tgcagggagg acaggatgtt cgctttcgac gacgagcagt
     9481 ttctagacgg ttatcgccgg cgcttcgggc cgctgcgcga cgaactcgcg caggccgtgc
     9541 gtttcctgat cggacgcatc gaacaggacg ccagcttcaa ccaaaacccg gtgcatcgcc
     9601 accagatcgc ctattgcctg gcgacgttca agtgggagac cgcgcatacc ttgcgcccca
     9661 tcgacgagtt cggcgacgac gcgcgtttcg aacgccttta cgggccgaat acgcgtgtcg
     9721 gcaagatgct cggcaatacc cgcgccggcg acggcgcgcg ttttcacggc cgcggttatg
     9781 tgcaactcac cggccgcgcc aactacgagc gcgccggcaa gatcctcggc atcgatctga
     9841 tcaaccatcc cgacgaagcc aagaagcccg aggtcgccta tgcgatcgcc atggaaggca
     9901 tgaagaaggg ctggttcacc ggcaagaaac tcgacagcta tttcaagccc ggacaacttc
     9961 cgaactacga ggacgcgcga cggatcatca acggccagga caaggcgcag acgatcgccg
    10021 acatcgcacg ccgcttcgac gagttgctgg tcaattcgct gcactgatcg cctcgcgacg
    10081 caggcgcgga cagtggcgca caccggcgct acaggaggtc gcgccggtgt gcaggcgacg
    10141 gtgcgaatcg ccgacgatcg attacgagct ggcgattgcg ggcaggcgga agctcaggcc
    10201 agcttcaagc cgaccaggcc ggcgacgatc aacagcacgc tggctacgcg cagcgtattg
    10261 agcggttcgt tgaacagcac gatgccgaga atgaccgtgc cgaccgcgcc gacgccgacc
    10321 cagaccgcat aggcggtgcc gaccggcagc gacttcatcg ccacgcccag caggccgaga
    10381 ctgatcgcca tcgcggcgat cgtgccgacc gatggccaca aacgggtgaa gccttcggtg
    10441 tatttcaggc cgatcgccca acccacttcg aacaggccgg cgaagaaaag cagaatccaa
    10501 ggcatgacga tgctcccatg atgatggggc cgtccccgga tgaagtgatg cgaacggggt
    10561 cgtccccgtt cgcgaacgat tataacgagc ccgattcggc ggggccggcc gcggtgttcg
    10621 cggggtcagc ggggggttac aaagcaagcg cagcgctgcg cttgcgggtt tcccgccctg
    10681 cctgcaggga cagccgacga cgacgatgct tcgtcgccgc atccgaccac tgcgacgcga
    10741 cgccgcgtcg caggacagtg gacggccctg ccccgaaggg gcgagcgcgt cctggcgcgc
    10801 atcaaccgcg ctggcgcacc gcctcgaaca aggtcacgcc ggcggccacc gagacattga
    10861 ggctttccac accgcc
//
