LOCUS       NZ_LMFT01000012        11293 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root55 contig_2, whole genome shotgun sequence.
ACCESSION   NZ_LMFT01000012
VERSION     NZ_LMFT01000012.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root55
  ORGANISM  Streptomyces sp. Root55
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 748558)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 748558)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140605
            Assembly Name                     :: Root55
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001427245.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 01:50:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,084
            CDSs (total)                      :: 7,008
            Genes (coding)                    :: 6,730
            CDSs (with protein)               :: 6,730
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 105 of 278
            Pseudo Genes (incomplete)         :: 225 of 278
            Pseudo Genes (internal stop)      :: 24 of 278
            Pseudo Genes (multiple problems)  :: 67 of 278
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:11:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 77014
            Orig. end                         :: 88307
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMFT01000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11293
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11293
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11293
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6293](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6293)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(481..496)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.0"
                     /tool="antismash"
     gene            546..2057
                     /gene="hemG"
                     /locus_tag="ASD51_RS13365"
                     /old_locus_tag="ASD51_04250"
     CDS             546..2057
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579252.1"
                     /locus_tag="ASD51_RS13365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04250"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_056791815.1"
                     /sec_met_domain="DAO (E-value: 3.1e-13, bitscore: 45.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQSPLQRDTTRTGHVVVIGGGIAGLAAAHRLLSTGLRVTVLEATE
                     RLGGKLMTGEIAGTRVDLGAESMLARRPEAVELATAVGLGGRLQPPATATASVWTRDAL
                     RPMPKGHVMGVPGDPAALSGVLSPEGLARIAQERDLTPTAVGDDVAVGAYVADRLGREV
                     VDRLVEPLLGGVYAGDAYRISMRAAVPQLFEAVKEGGSLLDCVRRVQERAAARQQTGPV
                     FQGIDGGIGTLPDAVADAVRAGGAEILLATPVLGLTRTARGWDVRTDTRVIDADGIVLA
                     TPAWSASTLLAAESPAASVELAAVEYASMALVTLAFRRSDIAGTQALHGRSGFLVPPVD
                     GRTIKASTFSSHKWQWVADAAPDLFVLRTSVGRYGEEEHLHREDSELVDVSLSDLAAAT
                     GLAARPVDTEVTRWIGGLPQYPVGHLTRVARIRDEVARLPGLRVCGAVYDGVGIPACIA
                     SARRAADEITRDLTGVDGGGNARKIAEEIIATPTRVQGTRSEAGQ"
     gene            2062..2775
                     /gene="hemQ"
                     /locus_tag="ASD51_RS13370"
                     /old_locus_tag="ASD51_04255"
     CDS             2062..2775
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888541.1"
                     /locus_tag="ASD51_RS13370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04255"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_056791817.1"
                     /transl_table=11
                     /translation="MSAPEKLPNAGKKAKDLNEVIRYTLWSVFKLRDVLPADRAGYADE
                     VQELFDQLAAKDVVVRGTYDVSGLRADADLMIWWHAETSDALQEAYNLFRRTALGRALK
                     PVWSNMALHRPAEFNKSHVPAFLADEEPRGYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGFPDVRANTVPSFSLGDYEWVLAFEADELYRIVDLMRHLRASEARLHVREEVPFY
                     TGRRKSVADLVSGLA"
     gene            complement(3041..3823)
                     /locus_tag="ASD51_RS13375"
                     /old_locus_tag="ASD51_04260"
     CDS             complement(3041..3823)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207254.1"
                     /locus_tag="ASD51_RS13375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04260"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_056791819.1"
                     /transl_table=11
                     /translation="MLWVVLLLVAWGAAAVSCIRLCLVTAGAARWPAEAAREPVLHELN
                     LYETAFLAGGPHRVADLALLSMHLRRRLLLAHTGWATVVDPEGRDEVERTVIRAIGPEG
                     QSRIAPIRASAAAADAVRTLADRLVAAGLAVPHGTRTTLASAVRAVRGAAVLVVALAVA
                     AVLLPGQDPAYAGPVVAWFGLPLVLTLGCLAIARVEAHPCSPWASPSGQRWLDSLPAPE
                     HGADRDLLAAVAVRGVAAVEDPELRAALRSGRSALSSH"
     gene            complement(3961..4995)
                     /locus_tag="ASD51_RS13380"
                     /old_locus_tag="ASD51_04265"
     CDS             complement(3961..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153333.1"
                     /locus_tag="ASD51_RS13380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04265"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_056791822.1"
                     /transl_table=11
                     /translation="MNALALLMTLAVAVSSTLLIVAAQSAGRRGGADGYVHDRSEAAFL
                     NGGPARVVDTALTALHTDGRLVIGGPGIVVLQRAEARDPVERAVLQELAAAPSGALHTL
                     RQAVMVHPAVQEIGDGLAARGLMVAPGESRNRRRWALVQGVGCTVALPVSLVLTFVQYA
                     LHEGYADMPFPFIVKVLPALVAGSIVGFLTAASARARVTGAGRRAAAGYRAAHAHVADP
                     AHLVATAGLRALVDPELRGQLMAAARHPSARRPSPSHSPSADSYAGGVVLPVMWCAGAG
                     PGNGSCGSSDGGGGHGTGSSCSSGSSCGGGSGGGSSCGSSSGSSCGSSSGSSCGGGSSC
                     GSSS"
     misc_feature    complement(4991..5004)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     gene            complement(5001..6293)
                     /locus_tag="ASD51_RS13385"
                     /old_locus_tag="ASD51_04270"
     CDS             complement(5001..6293)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153332.1"
                     /locus_tag="ASD51_RS13385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04270"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_056791824.1"
                     /sec_met_domain="DUF692 (E-value: 4e-94, bitscore: 310.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLGTGIGWRPEIEDAVEALPGIDWVEAVAENICTDHLPASLERL
                     RERGVTVVPHGVSLGLGGADRPDAGRLADLAARAVLLGTPLVTEHIAFVRAGGPLTASP
                     GLEAGHLLPVPRTWDALEVLCENVRIAQDALPVPLALENIAALVSWPGEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNRGEDPATALDELPVEAIAYVHVAGGVEKDGVWHDTHA
                     HPVTRPVLDVLAELRSRVDPPGVLLERDDDFPPAAELAGELTAIRATLATAAGTPAPAG
                     RRPARPSEPAGRTAGARLRVGTAQAALLSSLVAGAPAPEGFDHRRLGVQRRALAAKRAD
                     VVAKVAPELPEILGDGYRTAFLGYAGSRPMSAGYRRDALDFAEQLLVAGRPADDAARLR
                     LTHWWQDRAAPRPPRRTTRIARAARAALMGR"
     gene            complement(6416..7168)
                     /locus_tag="ASD51_RS13390"
                     /old_locus_tag="ASD51_04275"
     CDS             complement(6416..7168)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153331.1"
                     /locus_tag="ASD51_RS13390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04275"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_199862765.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATLGALAYPVWSYSDRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEAMKAAGDHLVVGHTDLDRRAR
                     DVAAKLGVELPNQPTEVQQGWLRELTAASGEEYERKFSNLLRAAHGKVFALIAEVRHTT
                     RNALIRQLASDANQTVLDHITMLERTGFVDFDGLAREAAGSSTASPSGPPMSSGGNVPQ
                     VPVPVTPSGDQSFTSRPVPPTMEPLPQP"
     gene            complement(7377..8129)
                     /locus_tag="ASD51_RS13395"
                     /old_locus_tag="ASD51_04280"
     CDS             complement(7377..8129)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523639.1"
                     /locus_tag="ASD51_RS13395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04280"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_056791829.1"
                     /transl_table=11
                     /translation="MSTPESPPGADREPVPADPADSPFRTEPTSRDEAPQFVLPLVVHI
                     EKTDPPARTDALRTAARAVLTMLSDERALGGGEWAQVMREWQDARIRKVVRRARGAEWR
                     KASALPGITVTGEHAEVRVFPPVPLDAWPRELAKLQVSGTDLDDPEPPAAPDRDAPVLW
                     MNPEVGMSAGKAMAQAGHGAQLAWWELSDTERKAWREAGFPLSVATPDPDLWRELTVSG
                     LPVVRDAGFTEIAPGSCTVVADHPALRR"
     gene            8235..8912
                     /locus_tag="ASD51_RS13400"
                     /old_locus_tag="ASD51_04285"
     CDS             8235..8912
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153329.1"
                     /locus_tag="ASD51_RS13400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04285"
                     /product="AIM24 family protein"
                     /protein_id="WP_056791831.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQQATAPGMTLQNSKSIKYAVNGEMHARQGSMIA
                     FRGDLQFERKGQGIGGMLKRAVTGEGLPLMAVRGQGEAWFAHEAANCFIVEMEQGDVLT
                     VNGRNVLCFDASLAYEIKTVKGAGMTGGGLFNSVFSGHGNLGLICDGQPIVIPVTAQQP
                     VYVDTDAVVGWSARLSTTLHRSQSFGSMVRGGSGEAVQLMLQGEGFVIVRPSELKPEKA
                     SAN"
     gene            8941..9120
                     /locus_tag="ASD51_RS13405"
                     /old_locus_tag="ASD51_04290"
     CDS             8941..9120
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049036.1"
                     /locus_tag="ASD51_RS13405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04290"
                     /product="hypothetical protein"
                     /protein_id="WP_056791832.1"
                     /transl_table=11
                     /translation="MDDSYCATPAPAPVPADAGPPYAACVLCRKPTEFPESTKGSTLCP
                     VCAWQEAGRTACSG"
     gene            complement(9161..10018)
                     /locus_tag="ASD51_RS13410"
                     /old_locus_tag="ASD51_04295"
     CDS             complement(9161..10018)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049037.1"
                     /locus_tag="ASD51_RS13410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_04295"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_056791835.1"
                     /transl_table=11
                     /translation="MTALMVLGAVLTGCGGRERSRTAALAPPASPGPSPGPGATVEARQ
                     GPPTMAPGPEGLTPVFERRAAGSRDGAPGADKTVALTFDADMTADQGPRASAGEHFDNP
                     GLIALLRRLKVPSTVFMTGRWAQEYPAQARSIGTDPLFEIANHSYSHYAFSSPCYGLPA
                     VAKADMRGDVERAFAAIRKTGARNVVPYFRFPGGCYDDASLKALAPTGVTAVQWDVVSG
                     DAFATDPEAVAEQVLSGVTPGSLVVMHCTRSAAPVTDEALERVVPELRERGYRFVKVSE
                     LMRG"
     misc_feature    complement(10559..10573)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 ttccgtggtc tcctccgaga cgaggccgcc ggccctctcc gcagcgccac gggcggcggc
       61 gaccgccttc ggcacgctcc tgacctcgac gtccagggtc gcggtgcgga tgacgtggac
      121 cgcggcggcg gcccccggct tcggcgcggc cggggagccg gccgcggact tgccggcggc
      181 cggcttgctg tccgactccg cgctgccgcc ctccgcgccg gatgccgggc cccgtcggtc
      241 ggaggccgcg acacccttct cgctcgacga gctgtcgaag gagtccgagg caccgcaggc
      301 gccggtgccc agcaggacga cgagcagccc ggcggccagg accgcgcgag aacgatgcgc
      361 ggggcgcagg ggcgggccta cgggtggatg acggtcggtg tggcggtcga tctgcatagc
      421 gagtcccccc agggccgttg ctcttgatgc cggttcgacg cacggggccc ctgtgcgggt
      481 ttcgccccag gggtttcgaa gcggtcacgt tcgggactcg gaagggggtt tgagagagtg
      541 gacccatgca gtctcctctt cagcgcgaca ccacgcgtac gggccacgtc gtcgtcatcg
      601 gcggtggcat cgccggactc gccgccgccc accggctgct ctccaccggt ctccgggtca
      661 ccgtcctcga ggcgaccgag cggctcggcg gcaagctgat gaccggcgag atcgcgggaa
      721 cccgcgtcga cctcggtgcc gagtcgatgc tcgcccgccg ccccgaggcg gtggagctgg
      781 ccaccgcggt gggcctcggc gggcggctgc agccgcccgc caccgccacc gcgtcggtgt
      841 ggaccaggga cgccctgcgc cccatgccca aggggcacgt gatgggtgtg cccggcgacc
      901 cggcggcgct cagcggtgtg ctctccccgg aagggctggc ccggatcgcc caggaacgcg
      961 acctcacccc gaccgccgtc ggcgacgacg tcgcggtcgg cgcgtacgtc gccgaccggc
     1021 tcggccgcga ggtcgtcgac cggctcgtgg agccgctgct cggcggggtg tacgcgggtg
     1081 acgcctaccg gatctcgatg cgcgcagcgg taccgcagct cttcgaggcc gtgaaggagg
     1141 gcggctcgct cctcgactgc gtccggcgcg tccaggagcg ggccgccgcc cggcagcaga
     1201 ccggaccggt gttccagggc atcgacggcg gcatcggcac cctgccggac gccgtggccg
     1261 acgccgtgcg cgccggggga gcggagatcc tcctggccac ccccgtgctc gggctgaccc
     1321 ggaccgcgcg gggctgggac gtacgcaccg acacccgggt gatcgacgcc gacggcatcg
     1381 tcctggccac cccggcctgg tcggcgtcca cgctgctcgc cgccgagagc ccggccgcct
     1441 ccgtggaact ggccgccgtg gagtacgcct cgatggccct ggtcaccctg gccttccggc
     1501 gttccgacat cgctgggacg caggcgctgc atggccgttc gggtttcctc gtcccgcccg
     1561 tcgacggacg cacgatcaag gcgtcgacct tctccagcca caagtggcag tgggtcgcgg
     1621 acgccgcccc ggacctcttc gtgctgcgca cctccgtcgg ccggtacggc gaggaggagc
     1681 acctgcaccg cgaggactcc gagctcgtgg acgtctcgct gagcgacctg gccgcagcca
     1741 ccgggctggc ggcacggccg gtcgacaccg aggtgacccg gtggatcggc ggcctgccgc
     1801 agtaccccgt ggggcatctg acgcgggtcg cccggatccg tgacgaggtc gccaggctgc
     1861 cggggctccg ggtctgcgga gcggtgtacg acggggtcgg catccccgcc tgcatcgcca
     1921 gcgcccgacg ggccgcggac gagatcaccc gggacctgac gggggtggac gggggcggga
     1981 acgcgcggaa gatcgcggag gagatcatcg ccacgccgac ccgggttcag ggcacccgga
     2041 gcgaggcggg acaatagccg tatgagtgcg ccagagaagc ttccgaacgc aggcaagaag
     2101 gccaaggacc tcaacgaggt cattcgctac accctgtggt ccgtgttcaa gctgcgagat
     2161 gtgcttcccg cggaccgcgc cggttacgcc gacgaggtcc aggagctctt cgaccagctc
     2221 gcggccaagg acgtcgtcgt ccgcggcacc tacgacgtgt ccggtctgcg ggccgacgcc
     2281 gatctgatga tctggtggca cgccgagacc tccgacgccc tccaggaggc gtacaacctc
     2341 ttccggcgca ccgctctggg ccgcgctctg aagccggtct ggtcgaacat ggcgctgcac
     2401 cgccccgccg agttcaacaa gtcgcacgtg ccggccttcc tggccgacga ggagccgcgc
     2461 ggctacgtca gcgtgtaccc cttcgtgcgc agctacgact ggtacctgct cccggacgag
     2521 gaccgccgcc gtatgctcgc ggaccacggc aagatggccc gcggcttccc cgacgtgcgc
     2581 gccaacaccg tgccctcctt ctcgctcggc gactacgagt gggtcctggc gttcgaggcc
     2641 gacgagctgt accgcatcgt ggacctcatg cgtcatctgc gggcctccga ggcacggctg
     2701 cacgtccgtg aggaggtccc gttctacacg gggcgcagga agtccgtcgc agacttggtg
     2761 tccggtctcg cataaccagc gtttccggtc accccgaccc ggaagatcag ttggcgggct
     2821 acggcgcgtg tgacgccgcc cgtcgttcca acgacaccgg gtccggctcc gggcacatcg
     2881 gcccagcgcc ggcgaactgc ggtggtctgc gggggagtca gccattcctc cccgcaggcc
     2941 ctcgctcctc gccgcgtgcg gccgatgtgc cccgggccga acaggcaccg catcggggcc
     3001 gctcccgtcg agggggcggc cctttcgccg tcccggcccg tcaatggctc gaaagtgccg
     3061 agcgaccgct gcgcagggcc gcccgcagtt ccgggtcctc cacggccgcc acgccccgta
     3121 cggccaccgc cgccagcagg tcgcggtccg cgccgtgttc cggcgccggg agcgagtcca
     3181 gccaccgctg cccggacggc gaggcccagg ggctgcaggg atgggcctcc acccgcgcga
     3241 tggccaggca tcccagcgtc agtaccagcg gcagcccgaa ccaggccacc accggccccg
     3301 cgtacgcggg gtcctgaccc ggcagcagca cggcggcgac ggccagcgcc accacgagca
     3361 cggccgcgcc ccgtaccgcc cgcacggccg acgccagggt cgtccgggtg ccgtgcggta
     3421 cggcgaggcc cgcggccacg agccggtcgg cgagggtgcg cacggcgtcc gccgcagccg
     3481 cggacgcccg gatcggcgct atgcgggact gcccctccgg gccgatcgct cggatcacgg
     3541 tgcgctcgac ctcgtcccgg ccctccgggt cgacgaccgt cgcccagccc gtgtgggcga
     3601 gcagcagccg gcggcgcagg tgcatggaga gcagcgccag gtcggcgacc cggtgcgggc
     3661 cgccggccag gaaggccgtc tcgtacaggt tgagttcgtg gagcacgggt tcacgggcgg
     3721 cctcggccgg ccaccgggcc gcgcccgcgg tgacgaggca gagacggatg caggagacgg
     3781 ccgcggcgcc ccacgcgacc agaagaagca cgacccagag catggcggat ttgtatgcga
     3841 gatgagtggg gggcgccact gtctgttcac gatatggacg aagtgtggtc acgcgcggtc
     3901 atcggtggct gaatccgcat gctcggcggc tgtcgccgcc cgcgacccgg atccgtacgt
     3961 tcaggagctg ctgccgcagc tggaaccacc cccgcagctc gacccgctgc tactgccgca
     4021 gctcgacccg ctgctgctcc cgcagctcga cccgccgccc gatcccccgc cgcagctcga
     4081 tccggaactg caggaggagc ccgttccgtg gccgccgccg ccgtcggaac tcccgcaact
     4141 gccgttcccg gggcccgcac cggcgcacca catcaccggc aggaccacgc cgccggcgta
     4201 cgagtcggcc gacggggagt gcgacggcga ggggcgccgg gcggagggat gacgtgcggc
     4261 tgccatcaac tgcccccgga gctcggggtc gacgagtgca cgcagccccg cggtggccac
     4321 caggtgggcc gggtccgcca catgggcgtg ggcggcccgg tatccggcgg cggcccggcg
     4381 cccggccccg gtgacccggg ccctggccga cgcggcggtg aggaagccca cgatgctccc
     4441 ggccacgagt gccggaagta ccttcacgat gaacgggaac ggcatgtcgg cgtagccctc
     4501 gtgcagggcg tactggacga aggtcagcac caggctgacc gggagggcca ccgtgcaccc
     4561 cacgccctgg acgagcgccc accggcggcg gttccggctc tcgcccggtg cgaccatcag
     4621 gccgcgggcc gcgagaccgt caccgatctc ctgcaccgcc gggtgcacca tcacggcctg
     4681 ccggagcgtg tgcagggccc cgctcggggc ggcggccagc tcctggagca cggcacgctc
     4741 caccgggtcc cgggcctcgg cccgctgcag gaccacgatg ccggggccac cgatgaccag
     4801 gcgcccgtcc gtgtgcaggg cggtgagcgc ggtgtccacg acccgggcag ggccgccgtt
     4861 gaggaacgcc gcctcggaac ggtcgtgcac gtacccgtcg gcaccgccgc gacggccggc
     4921 gctctgggcc gccacgatca ggagcgtcga ggagaccgcc acggcgaggg tcatcagaag
     4981 ggcgagggcg ttcatcgggg tcaccttccc atcagggcgg cacgggccgc ccgggcgatc
     5041 ctggtggtcc ggcgcggcgg acgcggagcc gcccggtcct gccaccagtg ggtcagcctc
     5101 agccgggccg cgtcgtcggc cggacggccg gcgacgagga gctgttcggc gaagtccagg
     5161 gcgtcacggc ggtacccggc ggacatgggc cgggacccgg cgtagccgag gaacgccgtg
     5221 cggtagccgt cgccgaggat ctccggcagc tccggggcca ccttggcgac gacgtcggcc
     5281 cgtttcgcgg ccagggcgcg gcgctgcacc ccgagccgcc ggtggtcgaa gccctccggg
     5341 gcgggcgcgc cggcgaccag tgaggagagc agcgcggcct gggccgtgcc gacccgcaga
     5401 cgtgcaccgg cggtccggcc ggccggctcc gacggccggg ccggccgccg ccccgcgggc
     5461 gccggggtgc cggccgctgt ggccagggtg gcgcggatcg cggtcagttc gccggccagc
     5521 tcggcggccg gcgggaagtc gtcgtcgcgc tccagcagga cgcccggggg gtcgacccgg
     5581 gagcggagct cggccagcac gtccaggacg gggcgggtca cggggtgggc gtgcgtgtcg
     5641 tgccagaccc cgtccttctc gacgccgccc gccacatgca cgtacgcgat ggcctcgacc
     5701 ggcagttcgt cgagggccgt cgcggggtcc tcgccccggt tcacgtggtt ggtgtgcaga
     5761 ttggccacgt cgatcagcag ccggaccccg gtccgctcga ccagttccgc gaggaactgc
     5821 ccctcggtca gctcctcgcc gggccaggag accagcgcgg cgatgttctc cagcgccagc
     5881 ggtacgggca gcgcgtcctg cgcgatccgg acgttctcgc agagcacctc cagcgcgtcc
     5941 caggtccggg gcacgggcag caggtggccc gcctccagcc ccggcgacgc ggtgagcggc
     6001 cccccggccc gtacgaacgc gatgtgctcc gtcaccagcg gtgtccccag cagcacggcc
     6061 cgcgccgcca ggtcggcgag gcggcccgcg tcggggcggt cggccccgcc gaggcccagc
     6121 gagacgccgt gcgggaccac ggtgacgccg cgctccctga gccgttcgag cgacgcgggg
     6181 aggtggtcgg tgcagatgtt ctccgcgacc gcctcgaccc agtcgatccc gggcagcgcc
     6241 tcgacggcgt cctcgatctc cggccgccag ccgattcccg ttcccagctt catcccgtcc
     6301 ccctcatcgg ctccgccgct tcgtacgggg gtgatggccc cgccgggcgg tggtgaatcc
     6361 gaaacagggg acgttcagag ctggatctga ggttcccggc gtcacgatgc cgtgatcagg
     6421 gctgtggcaa gggctccatc gtgggcggca cggggcgtga ggtgaacgac tggtcgccgc
     6481 tcggagtcac cggcaccggg acctgcggga cgttgccgcc cgaggacatg ggcgggcccg
     6541 aggggctggc cgtcgagctg ccggccgcct cacgggccag gccgtcgaag tcgacgaagc
     6601 cggtgcgctc cagcatcgtg atgtggtcca ggacggtctg gttggcatcc gaggccagct
     6661 gccggatgag cgcgttgcgc gtggtgtgcc tgacctcggc gatcagtgcg aagaccttgc
     6721 cgtgcgcggc ccgcagcaga ttggagaact tgcgctcgta ctcctcgccg ctcgccgccg
     6781 tgagctcgcg caaccagccc tgctgcacct ccgtcggctg gttcggcagc tccaccccga
     6841 gcttggctgc cacgtccctg gcccgccggt ccaggtcggt gtggccgacc accaggtggt
     6901 cgcccgccgc cttcatcgcc tcgctcggtg cccgctcgat cgcctgctgc cccgcgggca
     6961 gttcccacag ccccgcgagg cgcaccttga ccaggaagtc gcggtccgtc gccgagagcg
     7021 ggccccactg ggtggcgacg ctggaggcgt tcagattggc ctctccggtg cccgagcggt
     7081 ccgagtagga ccacacggga taggcgagtg cgccgagcgt ggcgaccagt gccgcgatga
     7141 tgagggccgt tccgttgatg cgtcgcaaca cgtatgtctc ccgggccgat gtgtgacagc
     7201 tctgaatcaa ccgccaactg actcggcgtt caacctactt ggcggtaatg ccggtgcggc
     7261 agtaccggga gatacgtaca tcggcgcagg gatgttcaac agcaccggcg gatcttccgc
     7321 cgggaggccg caccggctcg gacgcgcggc cccacgcccg ccccggggcc ccgcgttcag
     7381 cggcgcagcg ccggatggtc cgcgaccacc gtgcaggaac ccggcgcgat ctcggtgaag
     7441 ccggcgtccc gcaccaccgg cagcccgctc acggtgagct cccgccagag gtccgggtcc
     7501 ggggtggcca cggagagcgg gaatccggcc tcccgccagg ccttgcgctc cgtgtccgac
     7561 agctcccacc aggcgagctg tgcgccgtgc ccggcctggg ccatggcctt gcccgccgac
     7621 atgccgacct cgggattcat ccacagcacc ggagcgtccc gatcgggcgc ggccgggggc
     7681 tcggggtcgt ccaggtccgt gcccgacacc tggagcttgg ccagttccct gggccaggcg
     7741 tccaggggca ccggcgggaa cacccgtacc tccgcgtgct cgcccgtgac cgtgatcccg
     7801 ggcagcgcgg acgccttgcg ccactccgca ccccgggcac gccgcaccac cttgcggatc
     7861 cgggcgtcct gccactcccg catgacctgc gcccactcgc ccccgccgag cgcacgctcg
     7921 tcggagagca tcgtgagcac cgcgcgcgcc gcggtccgca gcgcgtcggt gcgcgccggc
     7981 gggtccgtct tctcgatgtg caccaccagc ggcagcacga actgcggcgc ctcgtcgcgg
     8041 gaggtcggct ccgtacggaa cgggctgtcc gcgggatccg ccggaacggg ctcccggtcg
     8101 gccccgggag gggactccgg ggtggacacg gtaaagggct cgggacggtc ttcgctgctc
     8161 acgggctcca gtctgccagg cccgcggaaa ccggtccttg ctggaacgaa ggggtccggg
     8221 tgaggatgcg gggcatgaag agcgacctct tttccagcga gcacatggcc cagcaggcca
     8281 ccgcccccgg tatgaccctg cagaactcca agtccatcaa gtacgccgtc aacggtgaga
     8341 tgcacgcgcg tcaggggtcg atgatcgcgt tccgcggcga tctgcagttc gaacgcaagg
     8401 gccagggcat cggcggcatg ctcaagcgcg cggtcaccgg cgagggcctg ccgctcatgg
     8461 cggttcgcgg ccagggggag gcgtggttcg cccacgaggc ggccaactgc ttcatcgtgg
     8521 agatggaaca gggcgacgtc ctgaccgtca acgggcgcaa cgtcctctgt ttcgacgcga
     8581 gcctcgcgta cgagatcaag acggtgaagg gtgccggaat gaccggcggc ggtctcttca
     8641 acagtgtctt cagcggccac ggcaacctcg gcctgatatg cgacggccag cccatcgtga
     8701 tccccgtcac ggcccagcag cccgtgtacg tcgacacgga cgcggtcgtc ggctggagcg
     8761 cccggctctc caccacgctg caccgttcgc agagcttcgg ctcgatggtg cgcggcggct
     8821 ccggcgaggc cgtccagctg atgctgcagg gcgagggatt cgtgatcgtc cgccccagcg
     8881 agctcaagcc ggagaaggcg tcggccaact gagcccgcgc gggtgagtac gctcggacgc
     8941 atggacgact cgtactgcgc gacgcccgct ccggcccccg tacccgcgga cgcgggcccg
     9001 ccgtacgccg catgcgtgct gtgccggaag ccgaccgagt tcccggagtc gacgaagggc
     9061 agcaccctct gcccggtctg cgcctggcag gaggcgggcc gcacggcctg ctccgggtga
     9121 cgcgggcggg cggcgtgcgc ggcgggtccg ccgtgtgtgc tcagccccgc atcagctcgg
     9181 agaccttgac gaagcggtag ccccgctcgc gcagctccgg gaccacccgc tccagcgcct
     9241 cgtcggtgac gggtgcggcg ctgcgggtgc agtgcatgac cacgagggat ccgggcgtca
     9301 ccccgctcag cacctgctcg gccaccgcct ccgggtccgt ggcgaacgcg tccccgctga
     9361 ccacgtccca ctgcaccgcc gtgaccccgg tcggcgccag ggccttcagc gacgcgtcgt
     9421 cgtagcagcc gccggggaag cggaagtacg gcacgacgtt gcgtgccccc gtcttccgga
     9481 tcgcggcgaa ggcccgctcc acatcgccgc gcatatcggc cttcgcgacg gccggcagcc
     9541 cgtagcaggg cgaggagaag gcgtagtggc tgtacgagtg gttggcgatc tcgaacagcg
     9601 gatcggtgcc gatcgagcgg gcctgggcgg ggtactcctg ggcccaccgc ccggtcatga
     9661 agaccgtcga gggcaccttc agccggcgca gcagcgcgat gaggcccggg ttgtcgaagt
     9721 gttcgcccgc cgaggcacgt ggcccctgat ccgccgtcat gtccgcgtcg aaggtgagcg
     9781 ccacggtctt gtcggccccg ggggcgccgt cgcgggagcc ggcggcccgg cgctcgaaga
     9841 ccggggtcag gccctccggc ccgggagcca tggtgggcgg cccctgtcgc gcctcgaccg
     9901 tcgcgccggg gccggggctg gggccggggc tcgccggggg cgcgagggcc gccgtgcgcg
     9961 agcgctcccg gccgccgcag ccggtgagga ccgcacccag caccatcagt gctgtcatcg
    10021 tacgaacgga tttgatcacc gggagacgtt agctgatgag gcatcagaat ccggtcgcgg
    10081 cactccgggc ctccacgtcc cgcaccaccg gcggcccctc gccgcgagct cccgcccgcg
    10141 gcgtcacggt gccgggaacc accgcggggc cgggcagcca ccgctgcccg gccccgcggc
    10201 ccgttacccg gccgctcagc gccaggggcc cgtcacggcg aacgtcgtgc ccggcgtgta
    10261 gcagttcacg aacatcgttc cgccgtccgg cgagaacgtg acccccgcga attcgcccca
    10321 ctccgggtca tcggccgtcc cggtgttctg ccgcccccgc gccatcggat agacctctcc
    10381 gcgccgcgtc accccgagca cgtgctgtgc gccgccgccg tcctcgcaca ccatcagccc
    10441 gccgtcggcg gcgaggcaga tgttgtccgg ggactcgccg ggcagctgga tgtccgtgtc
    10501 cgggccgaag acgaccacca gcgtgagccg gcgccgcttg ggctcgtacc gccagacctg
    10561 gccgaagtgg tcggcggccg acccctccga gctgtgagcg aagctggaga cgaagtagac
    10621 ggacgagccg ccccagtagc agccctccag cttctgggcg tgcgtgacgc ccctgggccc
    10681 gaagtcctgc agccggatcg gtgtctcggt ggcctgcgga tcgggcaccg ggacccactc
    10741 gacccggtcg aagctcgcac cggtctcctg gaccacggac aggtcgggca gtccggggac
    10801 cctcatggct tcgagcgcgc ccccggcccg cagcgagccc gtgccgccga gcggcttctc
    10861 gggcaggaaa cggtagaaga gcccgaacgg ccggtcgaac gcgtcctccg tctcgtacac
    10921 gaccccgttc ttcgggtcga ccgcgatcgc ctcgtgctgg aaacggccca tcgcggtcag
    10981 ggggactgcc ccggtgcggc gcggatcggc gccgtccacc tcgaagatga agccgtggtc
    11041 cttcgtgtag ccgttggtac cggccttgtc ctcggtctcc tcgcaggtca gccaggtgtt
    11101 ccacggggtg ggcccgcccg cgcagttgac cgccgtaccg gcgatcgcga ccctctcgcc
    11161 gagcacgttg ccccggctgt cgagctccag ggccgtacaa ccgcccttgc ccatcgggtc
    11221 gtacgtgagg ccctcgaccg tcgggacgcc gatcctgccg gtgacccggt tctcgtggtt
    11281 gcgcaccaga tgg
//
