LOCUS       NZ_LMFT01000010        10909 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root55 contig_18, whole genome shotgun sequence.
ACCESSION   NZ_LMFT01000010
VERSION     NZ_LMFT01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root55
  ORGANISM  Streptomyces sp. Root55
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 85198)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 85198)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140605
            Assembly Name                     :: Root55
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001427245.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 01:50:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,084
            CDSs (total)                      :: 7,008
            Genes (coding)                    :: 6,730
            CDSs (with protein)               :: 6,730
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 105 of 278
            Pseudo Genes (incomplete)         :: 225 of 278
            Pseudo Genes (internal stop)      :: 24 of 278
            Pseudo Genes (multiple problems)  :: 67 of 278
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:11:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 373
            Orig. end                         :: 11282
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMFT01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10909
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10909
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10909
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5909](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5909)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    1395..1409
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     misc_feature    complement(2806..2820)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.68"
                     /tool="antismash"
     misc_feature    3229..3244
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    3784..3798
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.72"
                     /tool="antismash"
     gene            4139..4909
                     /locus_tag="ASD51_RS12285"
                     /old_locus_tag="ASD51_30765"
     CDS             4139..4909
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (11-174). E-value: 3.8e-14.
                     Score: 44.9. Matches aSDomain:
                     nrpspksdomains_ASD51_RS12285_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047022.1"
                     /locus_tag="ASD51_RS12285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_30765"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_056791441.1"
                     /sec_met_domain="adh_short (E-value: 9.2e-37, bitscore:
                     119.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 3.8e-34, bitscore:
                     111.0, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTGGLLRDKVILVMGASSGIGAAAAYLFAREGASVVLAARREE
                     RLAALVAQLTAKGFEASSVVCDVTRADDVRRAVGHATERHSRLDGAFNNAGIGGDRAPL
                     HEMGDEVYDTIVDINVRGLWNCLRAEIGAMLASGGGAIVNNSSVGGLVAIPTAAPYVAS
                     KHAVVGFTRAAADEYARQGIRVNALAPGSTRSEITVDWYARNPGLEEAVHAVTPQGRTA
                     APEEVAEAAAWLLSDRSSYVTGTVLPVDGGYVNR"
     misc_feature    4157..4159
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        4172..4660
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD51_RS12285_PKS_KR.1"
                     /evalue="3.80E-14"
                     /label="ASD51_RS12285_PKS_KR.1"
                     /locus_tag="ASD51_RS12285"
                     /protein_end="174"
                     /protein_start="11"
                     /score="44.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VILVMGASSGIGAAAAYLFAREGASVVLAARREERLAALVAQLTA
                     KGFEASSVVCDVTRADDVRRAVGHATERHSRLDGAFNNAGIGGDRAPLHEMGDEVYDTI
                     VDINVRGLWNCLRAEIGAMLASGGGAIVNNSSVGGLVAIPTAAPYVASKHAVVGFTRAA
                     "
     gene            complement(5001..5909)
                     /locus_tag="ASD51_RS12290"
                     /old_locus_tag="ASD51_30770"
     CDS             complement(5001..5909)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="ASD51_RS12290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_30770"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_056791265.1"
                     /sec_met_domain="AfsA (E-value: 9.6e-19, bitscore: 59.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPKTLLSDRQRHSHVVRRIAAPVQRIPKEFIHLHRTEAVLLTNWS
                     TLGSGRYAVSAHWPRGGFFGDSVQLLLAQTIRQSCLLIGHAELSVPLPHQTLMEWMDFS
                     VSSDFYDPKNKPDNLFVLLTFKPNNPRSLRVELSILSEGRLVAESLLDFSWIAPGAYRR
                     LRGAHAEVPWGEVVVPSPVLPHTVGRAHEHEVLLAPTGLADRWELRMDVSNPALYDHRV
                     DHVPGLVLIEAAYQAAHAVTGPGHGPVRVMSTFDSYVEFDAPCLIDAAVVPAKDEDTVR
                     VEVTGVQSGERTFSITMDCAQ"
     misc_feature    complement(5700..5702)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(5954..5970)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.0"
                     /tool="antismash"
     gene            6015..6698
                     /locus_tag="ASD51_RS12295"
                     /old_locus_tag="ASD51_30775"
     CDS             6015..6698
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155347.1"
                     /locus_tag="ASD51_RS12295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_30775"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_241192063.1"
                     /transl_table=11
                     /translation="MTVAQQERAIRTRRVILATAAEIFDERGYESTTIAEILERADVTK
                     GALYFHFPSKEHLARGVLAAAVTTDGVRPQALKLQELIDIVLLLAYRLPREPVLSAALR
                     MAIDPQSRRQFGTRWVDWTELLTALLKEAKERGEVCADVDETETARMLVCCWTGVQVVT
                     EGLPGSYDLAGEIASLLRRFMPTIAEPGVMSRLTVSAERAAHLHDDMPAWRSETGESEF
                     VGLTP"
     gene            complement(6713..7543)
                     /locus_tag="ASD51_RS12300"
                     /old_locus_tag="ASD51_30780"
     CDS             complement(6713..7543)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763580.1"
                     /locus_tag="ASD51_RS12300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_30780"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_056791270.1"
                     /transl_table=11
                     /translation="MRFNLIGPLQIIADNGSVLPVGSPKVSQVLAVLLTHSNDLVTPDT
                     FIQELWRHGPPRSALNTVQTYVHHARRLLTQDARPGTEHSLLTTRFGGYTMRVDDSEID
                     IKIFERLVARGNSELRAGRYEAAADALGMSLDLWHGPVLSNVPTGDVLAGRIVHLQELR
                     IRALELRVEAMQRLGRLRELIPELRTLVHDYPLNEWFHGQLIAALHQAGRRAEALDAYQ
                     KVRRLLREELGLEPSRDIQWLHESVLSPVRVPPGPTTPLGGGVVVPMWDSARSG"
     misc_feature    complement(7517..7519)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7630..8793)
                     /locus_tag="ASD51_RS12305"
                     /old_locus_tag="ASD51_30785"
     CDS             complement(7630..8793)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /locus_tag="ASD51_RS12305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_30785"
                     /product="transposase"
                     /protein_id="WP_056791273.1"
                     /transl_table=11
                     /translation="MHSPSFHQMLAEIPMDLFDSLPRNDQRSKAGEYVAGLLGAHGRKT
                     LRNVASQLGGQAARSQSIHHFISESSWEWEPVRRGLARRADELLGPRAWVVRPTVVPKG
                     GGHSVGVAEYFAPQLGRTVTAQQSFGAWLVSPAAGVPVNWQLALSADWTEELRRRRARI
                     PATSRALSPEENAGDVALRAVRARSGGPLPVVLDAPGLDPLRMVRRFGRAGVPLVQRVE
                     QRLRLRVGPLALPGHGDVPLSAGRLVGSMRQLWRARPAAGGRSLSMAVAVVLPGAPDGG
                     REAGGQRLLVAQWPESDLSAARLWLADSRLGHAGVLRLAGLAEVVDRDDALVASRVGLR
                     DFAGRSFQGWHRHITLASVAHLAYMRAALTTAAPHRTPLVPLLPGAG"
     misc_feature    complement(8092..8094)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(9232..10074)
                     /locus_tag="ASD51_RS12310"
                     /old_locus_tag="ASD51_30790"
     CDS             complement(9232..10074)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015580402.1"
                     /locus_tag="ASD51_RS12310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_30790"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_056791275.1"
                     /transl_table=11
                     /translation="MTGQRPDRDAAARKLGAALRSLQQRSGCTLRGLETRVRISDSSLS
                     RYFRGETVPAWPVVRDLCRALGADPAEYRALWEATGQAPPDEPVAAVVRRTPEESGAGE
                     DAVGPSRSRGGPARWHRWRGPLSGRGVIAVAAAAAGGLVAVGLLLFLLPSQGVPGAAPG
                     ARGAEGGSGRSGDAGVLVHNVEEVCQEPRTTSCALSLAYSPYRPYVTTNAADRVWHHDL
                     LRARCTVANGVTVTDENHKHTSIWIQVTHGGRRLWLPGIRVHPDSLTQLTTTLPLCSS"
     gene            10342..10623
                     /locus_tag="ASD51_RS12315"
                     /old_locus_tag="ASD51_30795"
     CDS             10342..10623
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140661.1"
                     /locus_tag="ASD51_RS12315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_30795"
                     /product="hypothetical protein"
                     /protein_id="WP_056791278.1"
                     /transl_table=11
                     /translation="MRVRTALSAFAAMAVVGLGLVSAAPAVAVSGAASHPVAAVQAADG
                     LDIHRGKYMTLGGCQTAGQQGIERGHWDRYQCAEGSAWPWVWNLWTNR"
ORIGIN
        1 cggtcgtcac ggcactcggc ctcctccacc agcacggcgt ccccgtcgac tggaaggcgt
       61 tcttctccgg caccggtgcc cgccggatcc cactgcccac ctacgccttc cagcaccagc
      121 gctactggat cgactcggcg gccgcagccc cccgcaccgc cggacacggc ctccgcccga
      181 ccggccaccc cctcctcggc gccgccgtca ccctcgcggg cgccgaccgg accgtgttca
      241 ccagcacctt ctccgcccgc acccacccct ggctcgccga ccacaccgtc tccggttccg
      301 tggtgctggc ccccgcagcg ctcgtcgagc tggccgtccg ggccggcgac gagtccgggg
      361 ccaccaccct cgacgaattc gtcatggaca ccccgttcgt cctcggcaag gcccgcaccc
      421 accagctcca ggccgaggtc ggcgcccccg accccacagg ccgccgcccg gtcaccgtgc
      481 acggccgccc cgacgacccc gacgcaccat ggaccatgca cgcccacggc acgctcggcg
      541 tcaccgccca gggccccgcg ttcgagccgg ccccgtggcc gcccgccgac gccgaggaga
      601 tcgccctcga cgagctgcac gccggactcg ccgaagcagg cctcgcatac gggcccacct
      661 tccaggtcct caccgccgcc tggcgccgcg gcggcgaact cttcgccgaa gccgccctgc
      721 cccagccgac cggcaccgac ggcttcaccc tgcaccccgc actcctcgac gcggcggtac
      781 gccccctcct gcccgccgtg acgaagggga gcccgctcgt cggccactgg cacggcgtac
      841 ggctctacgc gaccggtgcc accacactcc ggatcggcat caccccgggg gacggcggcg
      901 cacgcataga aatagccgac ccttccggac agcccgtggc cctcatcacc gcggcgtcgg
      961 cgcgcggtgt cgaggaccgc gcgttcgcgg cggccgccga ccggcacgac gacgcgctgt
     1021 tccacatggg ctggacaccc ctggccgtag aagcggcgga gggcatcacc cccgccgcac
     1081 tcggcgacga cggcgacttc accagccccg ccgaagtcgc cgcggccgtc gccgccggcc
     1141 ggacagtccc cctggtcgtg ctccgcaccc ggccggaacc ggcgggctcc ctgcccggct
     1201 cggtccacgc cacgacggag aaggtcctga agctggtgcg cgagtggctg gccgacgagc
     1261 gcctggagga gacccggctc gtggtcgtca cctcgggagc ggtcgccacc cgtgaagggg
     1321 aggacgtcac cgacctggcc ggcgccgccg tctggggcct gctccgctcc gcccagtcgg
     1381 aagcccccga ccgcatcatc ctcatcgaca ccgacaccga caccgacacc gacaccgaca
     1441 ccgacaccga caccgacacc gacaccgaca ccgacaccga caccacgccg gcccgcaccg
     1501 acaccggcac ggcccgccga ccctgccccg cgaacggcgg gcacgtcgtc gccctcgccg
     1561 cctccggtga accccaggcc gccctgcgcg ccggacaggt gtacgtcccc cgcctcgcac
     1621 gggccgtcac cccccgcccc ggcaccgccc cggcctggaa ccccgacggc acggtcctga
     1681 tcaccggcgg aaccggcagc ctcggagccc tcttcgcccg ccacctcgtg accgcacacg
     1741 gagtgcgcca cctcctgctc accagccgca ccggccccgc ggccgccggc gcccaggaac
     1801 tgtcggccga actgaccgcc ctcggagccc gggtcaccat cgccgcctgc gacacggccg
     1861 accacacggc actcgccgaa ctcctcggca cgatccccga cagcctcccg ctcaccggcg
     1921 tcgtgcacgc cgccggcgtc ctggacgacg gcctcgtcac caccctcacc cccgaacggc
     1981 tgcaccgcgt gctgcgcccc aaggccgata ccgcctggaa cctgcaccgc ctcacccagg
     2041 acaaggagct caccgccttc gtcctcttct cctccatcgc cgcggtcgtc ggcggccccg
     2101 gccagtccaa ctacgccgcc gccaaccagt tcctggacgc cctcgcccag caccggcacg
     2161 cacacgggat gcccgccacc tccgtcgcct gggggctctg ggagcagtcc ggcggcatga
     2221 gcggccacct cggcgagagc gacctcgccc gcatcgcccg cagcggattc cgccccgtcc
     2281 cccaggacca gggacccgcc ctgctcgacc gggcgatcgc cctcgggcac ccggccgtcg
     2341 tcgcgacccc cctggacatc gccgtcctgc gcgaacagcc cggccgcatc ccggcagtcc
     2401 tcaccggtct cgcacccgcc cccgcacgcc ggaccgccgt cgagaagggc acacccgcaa
     2461 ccgcctcgct cgccgacctg gtcgccgggc tcgaggggcc agagcgcgaa caggccgtcc
     2521 tggaagcggt tctcgacctc agcgcggccg tactcggcca cgccgacacc tccggcatcc
     2581 aggccggaca gccgttgacg aaactgggct tcgactcgct gacctccgtc gatctccgca
     2641 accggctgag cgccctggcc ggctccaaac tgcccgccac actggtcttc gaccacccca
     2701 cccccgccgc cctcgccgcc ttcgtctgcg ggcagctcgc cggcgggccc ggcccggaca
     2761 ccaccccggc cccgcccacc gtggacttcg cggccgaggc gcgcctcgcc gacgacatcc
     2821 gcccggcggc cgggaccgta cggccggccg aggagccgca ctcgatcctg ctgaccggag
     2881 ccaccggctt cctcggcgcc ttcctgctgc gcgacctgat gcggacgacc acggcgacca
     2941 tccactgcct ggtccgcggc gccgaccagg acgaggcgag ggaccggctg cggaccaaca
     3001 tgcagtggta ccggctgtgg gacgaggtcg acgagagccg gctctccctc gtcctgggcg
     3061 acctggccga ggagcggctc ggcctcacgc aggagcgctt cgacgccctg gcccacacgg
     3121 tggacgccgt ctaccacaac ggcgcccggg tccactggct gcatccctac acggcactgc
     3181 gcgacgccaa cgtccgcggc accgaggagg tgctgcgcct ggcggcccgt caccgcaccg
     3241 tccccgtcca ctacgtgtcc accgtcggcg tcttcgacgg cgtacgggag gagggcgtcc
     3301 ccctgaaggt gaccgacccg acgggccccg ccgaggcact gcccagcgga tacctccaga
     3361 gcaagtgggt cgccgagcag ctcatcggca ccgcccgcga ccgggggctg cccgtctccg
     3421 tctaccgggt cgacgtgatc tccggagaca cacgcaacgg cgcctgccag acccgcgact
     3481 tcgtatggct cagcctcaag ggcatcctcc agtccgcggc ggtgcccggc ggcaccggtg
     3541 gccgcttcca cctgctgccc gtcgactacg tcagcgccgc catcaccacc ctctcccggc
     3601 agccggacac cgccggccgc acgttccacc tgttcaacca gagctccctc agcctcgccc
     3661 agtgcgtgga gaacctgcgg gcgcacgggt acgcgctgga cgagcaggac tggcccagct
     3721 ggaacgagag ggtcagcgcg agcagcgaca acgcactgcg tccgctgctc cacgccttcg
     3781 agatgatgac ctcggacacc gacggcttct acccgccgat cgacacctcg gagaccctcg
     3841 cggcgctcgc cgggacaggc gtcgactgcc cgccgctcac cgacggcctc ttcgcccggt
     3901 acgtcgactt cttcgtcgag gtgggccact tcccacccgc gccgggcagg gagcaggcgc
     3961 gcgcctgacc ggaggtccgg gccggcagcc gggcgaagcg cccggcccgg acctcccccc
     4021 ggcaccctgg gacccggggg aggtccgggg cccggacctc ccccgacctg acataccctg
     4081 ccccggcctt gcgggaatgc gggccggcgg cggaccgccc gatggcagca tcggcgccat
     4141 gagcgatacg ggtggattac tgcgggacaa ggtcattctg gtcatgggcg cgagcagcgg
     4201 gataggcgcg gcagccgcgt acctcttcgc ccgcgaaggc gcgtcggtgg tcctcgccgc
     4261 acgccgagag gagaggctcg ccgctctcgt ggcgcagctg accgccaagg ggttcgaggc
     4321 ctcctcggtg gtgtgcgacg tgacgcgcgc cgacgacgtg cggcgcgcgg tcgggcacgc
     4381 cacggaacgc cacagccggc tcgacggtgc cttcaacaac gcgggcatcg gcggggaccg
     4441 ggcccctctc cacgagatgg gggacgaggt ctacgacacc atcgtcgaca tcaacgtgcg
     4501 cgggctgtgg aactgcctgc gcgccgagat aggcgcgatg ctcgcgagcg gcggcggtgc
     4561 catcgtcaac aacagcagcg tgggaggcct ggtcgcgata cccaccgccg ccccgtacgt
     4621 ggcgtccaag cacgcggtgg tcggcttcac ccgcgccgcg gccgacgagt acgcccgcca
     4681 gggcatccgc gtcaacgcgc tggcgcccgg ttcgacccgc agcgagatca cggtggactg
     4741 gtatgcgcgc aaccccggcc tggaggaggc ggtccacgcg gtcacccccc agggccgtac
     4801 cgccgcgccg gaggaggtgg ccgaggccgc ggcctggctg ctcagtgacc ggtcctccta
     4861 cgtcaccggc accgtgctgc cggtggacgg cggttacgtc aaccggtgag cacagccccg
     4921 ggaacggggc ggggaaaccg aggagggggc ggtgccggtc cgtacggacc ggcaccgccc
     4981 cctgtgccgc gggccggatc ctactgcgcg cagtccatgg tgatggagaa ggtgcgctcg
     5041 ccgctctgca cgccggtgac ctcgacgcga acggtgtcct cgtccttcgc ggggacgacc
     5101 gcggcgtcga tcaggcacgg cgcgtcgaac tcgacgtagg agtcgaaggt gctcatgacc
     5161 cggacggggc cgtgtcccgg accggtgacc gcgtgggcgg cctggtacgc cgcctctatc
     5221 agcacgagac cgggcacatg gtcgacccgg tggtcgtaca gcgcggggtt ggacacgtcc
     5281 atgcgcagtt cccagcggtc cgcaagcccc gtgggagcca gcaggacctc gtgctcgtgg
     5341 gcgcgcccca ccgtgtgcgg caggacgggg gagggaacga cgacctcccc ccacgggacc
     5401 tcggcgtgtg ccccgcgcag ccggcggtag gcgcccgggg cgatccagct gaagtcgagg
     5461 agcgactccg cgaccagccg gccttccgag aggatcgaca gctcgacccg cagtgacctg
     5521 gggttgttcg gcttgaacgt cagaagaacg aacaggttgt ccggtttatt ttttggatcg
     5581 taaaaatcgc tggagacgct gaagtccatc cactccatca gcgtctggtg cggaagcgga
     5641 accgagagtt cggcgtgccc gatgagcagg cagctctgcc ggatcgtctg ggcgagcagt
     5701 aactgcaccg agtctccgaa gaatccgccc cggggccagt gcgcgctcac ggcgtagcgg
     5761 ccggatccga gggtggacca gttcgtgagg aggacggctt ccgtccggtg caggtggatg
     5821 aactctttgg ggatgcgctg tacgggtgcc gcgatccggc ggactacgtg gctgtgtctc
     5881 tggcgatcgg aaagcagtgt cttgggcatg atccccccct gggtcaggcc gagatgaggc
     5941 gccggggttc ggcacgggat aatataccgc gcaagcggtc cttttttcga taggtaagaa
     6001 cacaaacacc aggggtgact gtggcgcagc aggaacgagc gatccggacc cgaagggtca
     6061 tcctggcgac ggccgccgag atcttcgacg agcgcggcta cgagtcgacg accatcgccg
     6121 agatcctgga gcgggccgac gtgaccaagg gggccctgta cttccacttc ccgtccaagg
     6181 aacacctggc acgcggcgtc ctggcagccg cggtgaccac ggacggagtc cgcccccagg
     6241 ccctcaaact gcaggaactg atcgacatcg tcctcctgct ggcctaccgg ctgcctcgtg
     6301 aacccgtact gagcgccgcc ctgcgcatgg ccatcgaccc ccagtcccgg cgccagttcg
     6361 gcacccgctg ggtcgactgg accgagctgc tcaccgcgct cctcaaggag gccaaggagc
     6421 gcggggaggt ctgcgcggac gtcgacgaga cggagaccgc ccgcatgctg gtgtgctgct
     6481 ggaccggtgt ccaggtggtc accgagggcc ttcccgggag ttacgacctg gcgggcgaga
     6541 tcgcctccct cctgcggcgt ttcatgccga ccatcgcgga acccggcgtc atgtcccggt
     6601 tgacggtctc ggccgagcgg gcggcccatc tccacgacga catgcccgcc tggcgttccg
     6661 agacggggga atccgagttc gtcggtctga ccccctgacg tcgagggggc gttcatccgg
     6721 atcgggccga atcccacatg ggcaccacga cgccgccacc gagcggcgtg gtgggacccg
     6781 gcgggacccg gaccgggctg agcacgctct cgtgcagcca ctggatgtcg cgcgacggct
     6841 cgaggcccag ctcctcccgc agcaggcgcc ggaccttctg atacgcgtcc agcgcctcgg
     6901 cccgccgtcc cgcctggtgc agggcggcga tcaactgacc gtggaaccac tcgttgagcg
     6961 ggtagtcgtg cacgagcgtc cgcagctcgg ggatgagttc acgaagccgt ccgagccgct
     7021 gcatggcctc gacccgcagc tccagcgccc tgatccgcag ctcctgcagg tggacgatcc
     7081 tgccggcgag gacatcgccc gtcggcacgt tggagagcac ggggccgtgc cacaggtcga
     7141 gcgacatgcc gagggcgtcg gcggcggcct cgtaccggcc cgcccggagc tcgctgttcc
     7201 cccgggcgac gagccgttcg aagatcttga tgtcgatctc ggagtcgtcg acgcgcatgg
     7261 tgtacccgcc gaaccgggtg gtcagcaggg agtgctcggt gccgggacgg gcgtcctgcg
     7321 tgagcagtct tcgcgcgtgg tgcacatagg tctgcaccgt gttgagcgcc gaacgcggcg
     7381 gaccgtgccg ccacagctcc tggatgaagg tgtccggcgt cacgaggtcg ttggaatggg
     7441 tgaggagcac ggccaggacc tgggagacct tggggcttcc cacggggagg acggaaccgt
     7501 tgtcggcgat gatctgtaac ggtccgatca ggttgaatcg catgagcact ctccttcgat
     7561 gcgaacgtct tcggcggtcc ggcgtacggc gggcggccgg ccgggggaga agagggaggg
     7621 ccggccggat cagccggccc ccggcagcag cggcaccagg ggcgtccggt gcggtgccgc
     7681 agtggtgagg gccgcccgca tgtaggcgag atgggccacc gacgccagcg tgatgtgacg
     7741 gtgccagccc tggaaggacc gtccggcgaa gtcccgcagc cccacgcgtg aggccaccag
     7801 ggcatcgtcg cggtccacga cctcggcgag cccggccagc cggagcaccc cggcatgccc
     7861 cagccggctg tccgccagcc acaaccgtgc ggcggacagg tcggattcgg gccactgggc
     7921 caccaggagc cgctgcccgc ccgcctcccg gccgccgtcc ggcgcccccg gcagcacgac
     7981 cgcgaccgcc atggacaagc tcctgccgcc cgctgcgggc cgggcccgcc agagctgccg
     8041 catcgagccg acgagccggc ccgcgctgag tggcacgtcc ccgtggccgg gtaacgccaa
     8101 gggccccacc cgcagccgga ggcgctgctc gacacgctgc accagaggga ccccggcccg
     8161 cccgaagcgc cgcaccatac gcagcggatc gagccccggc gcgtccagca ccacgggaag
     8221 gggacccccg gaacgagcgc gcacggcacg cagggccacg tcgcccgcgt tctcctccgg
     8281 actcagcgcc cggctcgtcg ccgggatgcg cgcccgccgc cgacgcagct cctcggtcca
     8341 gtcggccgac agggcgagct gccagttcac cggcacaccc gcggccggcg agaccagcca
     8401 ggccccgaag gactgctgcg cggtgacggt ccgtccgagc tgcggcgcga agtactccgc
     8461 cacaccgacc gaatgcccgc cccccttggg gaccacggtg gggcgcacca cccaggcccg
     8521 gggcccgagc agctcgtccg cacgccgggc gagaccgcgg cgcacgggct cccactccca
     8581 cgaggactcg ctgatgaagt gatggatgct ctggctgcgc gccgcctgcc cgcccaactg
     8641 gctcgccacg ttgcgcaggg tcttgcgccc gtgcgcgccc agcagcccgg ccacgtactc
     8701 cccggccttc gaccgctggt cgttcctcgg cagggagtcg aacaggtcca tgggtatctc
     8761 ggcgagcatc tggtggaacg agggcgagtg catgtcccat aactccctgc gaaaccctgg
     8821 tgtcctgttt tcggtcatgg cgcgccgacg gcaggccgcg ccgcgcgcga agccgcgtgg
     8881 cgagccgtca tcaaatgaaa ccacacacgc ggtttgttgt gttgtcctcg tgaagcctta
     8941 cccgtgcaga gcgaacccgc cacccccgcc gaccagggcg gggcgacggg aaagaaccgg
     9001 acacccgctc ctcgcgaaag ccgcggccca agtgccggga accccgccgc ccctggccct
     9061 ggggccgtca tgctgctgca gccactgacg acgcacgcga cgggtacgcc gtcctgcatc
     9121 acggtcacga cgaactgcgc acatggggcc gctacgccca cgggggccgg gcagggctga
     9181 cgccgcatcc gacggccgtg ccgcccaccc cctgcacccc cactcccgca cctacgagga
     9241 gcacaggggc agggtggtgg tgagctgggt caggctgtcg gggtggaccc ggattccggg
     9301 cagccagagg cgccttccgc cgtgcgtgac ctggatccag atgctggtgt gcttgtggtt
     9361 ctcgtcggtg acggtgaccc cgttggcgac ggtgcagcgg gcgcggagca ggtcgtggtg
     9421 ccagacacgg tcggccgcgt tggtcgtgac gtaggggcgg taggggctgt aggccaggga
     9481 gagcgcacat gaagtcgtgc gcggctcctg gcagacctcc tcgacgttgt ggacgagaac
     9541 acccgcatca ccactcctgc ccgaaccgcc ctcggcgccc cgtgcgccgg gtgcggcacc
     9601 cggcaccccc tgcgagggaa gcaggaacaa caggagcccc accgccacga gtccgcccgc
     9661 agcggccgcg gccaccgcga tgaccccccg gccggacaac ggcccacgcc atcggtgcca
     9721 ccgggccggg ccgccgcggg agcgtgacgg accgacagca tcctcaccgg cacccgattc
     9781 ctccggagtg cgccggacga ccgcggcgac gggctcgtcg ggcggggcct ggccggtcgc
     9841 ctcccacagg gcgcggtact cggcaggatc cgcccccagc gcccggcaca ggtccctcac
     9901 cacgggccag gccggcacgg tctccccccg gaagtagcgg gagagcgacg agtcgctgat
     9961 acggacccgg gtctccaggc cgcgcagcgt gcaccccgat cgctgctgca ggcttcgcag
    10021 cgccgctccc agcttccgtg ctgccgcatc acggtcgggc cgttggccgg tcatcgaagt
    10081 cgccctttcc gcagggcggg gcaacggcgt cccgccgcct gtcccgcgtc ccgggacgga
    10141 tgcgtcccag caggtcagga ctgtatttat cccgacatcg gtgaagcaag cgggatctgg
    10201 ttgtaaaggt gcggcttcct gcgcaaactc ggcgtaccgg ctgaacagat cacccccagg
    10261 ggtgagcgtg ccggtggctg tggccgtgcc ggcggatgcc gcacgtgccg acttcgtatc
    10321 cctaccggaa ggatcttcac catgcgtgta cgcaccgcac tgtccgcttt cgcagcgatg
    10381 gccgtggtcg ggctcgggct ggtctcggcg gcacccgccg tcgcggtgtc cggggcggcg
    10441 tcccacccgg tggccgccgt ccaggcggcc gacggcctcg acatccaccg aggcaagtac
    10501 atgaccctgg gcggctgcca gaccgccgga cagcagggca tcgagcgcgg acactgggac
    10561 cgctaccagt gcgccgaggg ctcagcctgg ccctgggtgt ggaacctgtg gaccaaccgc
    10621 tgagcacatg acgccggcgg tggcctcccc cacgcaggag aagccaccgc cggcccgcac
    10681 cggtccgtac gccgtgtgcc cccggacgcc gtaccccgcc cgtgccgccg ggtacagcgc
    10741 ccaggggccg caaccccgaa gggcattgac cgaatccgga cccgggccgg tacgcaccgg
    10801 tgccgtgacc gagccgtctc ctgccgcccg gcagccgaac cgccccgggc ggtacgagac
    10861 gaccacgtgc cacggcctcc acgggctggt cgcgttcccc ggccggcgc
//
