LOCUS       NZ_LMFT01000003        10471 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root55 contig_11, whole genome shotgun sequence.
ACCESSION   NZ_LMFT01000003
VERSION     NZ_LMFT01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root55
  ORGANISM  Streptomyces sp. Root55
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 267152)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 267152)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140605
            Assembly Name                     :: Root55
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001427245.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 01:50:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,084
            CDSs (total)                      :: 7,008
            Genes (coding)                    :: 6,730
            CDSs (with protein)               :: 6,730
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 105 of 278
            Pseudo Genes (incomplete)         :: 225 of 278
            Pseudo Genes (internal stop)      :: 24 of 278
            Pseudo Genes (multiple problems)  :: 67 of 278
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:11:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 140909
            Orig. end                         :: 151380
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMFT01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10471
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10471
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10471
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5471](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5471)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            677..1813
                     /locus_tag="ASD51_RS06960"
                     /old_locus_tag="ASD51_25440"
     CDS             677..1813
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018487589.1"
                     /locus_tag="ASD51_RS06960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25440"
                     /product="glycosyltransferase"
                     /protein_id="WP_056787918.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3.7e-20,
                     bitscore: 66.1, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKALHIITGLGVGGAEQQLRLLLRHLPVRCDVVTLTNPGAVAEGL
                     RADGIRVRNLGMRGNRDLTAVPRLARLIRRGAYDLVHTHLYRACVYGRIAARIAGTTAV
                     ATEHSLGRADIEGRPLTRGTRALYLRTERMGAATVAVSGTVAGRLRDWGVPAARIHTVA
                     NGIDVAAFRYDGAGRRTTRASLGVPQDAFVVGGVGRLVPGKRFDLLIRAVAALPGAWLV
                     LAGDGPEAGALRVLAARSGIADRVRFLGECGAGNASPGIPEALWAMDTFVSASREETFG
                     LAVVEALAAGLPVVHGACPAVDDLPADRAPGTTRISSLTDPVGELTTALRHRMESGTRR
                     LPPPPAADHYDIRLSSERLMTVYAHALDTAPPTERAHS"
     misc_feature    695..709
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     gene            1810..2484
                     /locus_tag="ASD51_RS06965"
                     /old_locus_tag="ASD51_25445"
     CDS             1810..2484
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157954.1"
                     /locus_tag="ASD51_RS06965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25445"
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_056787922.1"
                     /transl_table=11
                     /translation="MTDSPEPKPAVPGRLRRLRPASVRPTWWPLPACVLLGAACGLSYG
                     LLAQPEYASTSYAVVTAGKGIDPAAALGYAQSYGRLATSDATLGYARGAAGEPVRELRT
                     HVRSETSPDSPMIAITGTSADPRRAADIANAVTEAVVVSSGHVAKDTGVKLIKFSHAVR
                     PVAPVSPSVPLGTAVGGSAGGLVGGLVLLVRPRPGRRGVTAQVPGPSPAAAAPAGEREL
                     VR"
     gene            2481..3647
                     /locus_tag="ASD51_RS06970"
                     /old_locus_tag="ASD51_25450"
     CDS             2481..3647
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157953.1"
                     /locus_tag="ASD51_RS06970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25450"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_056787924.1"
                     /transl_table=11
                     /translation="MTSDRDGRLTVTLCRDLREFSALAAEWDALHRRCPTATPFQSHAW
                     LHSWWLSYGVPGRLRIVLARRGERLVGAAPLMLAHRPMPLLVPMGGAISDFFDILVDGA
                     CTHDAVAALERGLDRAAVHTVIDLREVRPDASAQLLFALWPGARSRLTDSTCMELPALP
                     ITALIERMPGARGQRVRAKLRKTDAAGIECRAVTGHRVAAAVDTLLRLHALQWRGRGVN
                     AEHLRPRFRAHLVRSTRRMVRDGEASLREFLLDGEVVAADLSLRSALLTGGYLYGADPR
                     LRERKVDVSSMLLREVAEQAAGTGSGALSLLRGSEPYKNHWAPVAVVNQRLLLARSVLE
                     PLLRLRESQVVVRDRAAETVKTRFPAARDWRGRLSGLPSSGLLAMGRN"
     gene            3759..4034
                     /locus_tag="ASD51_RS06975"
                     /old_locus_tag="ASD51_25455"
     CDS             3759..4034
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575906.1"
                     /locus_tag="ASD51_RS06975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25455"
                     /product="chaplin"
                     /protein_id="WP_056787928.1"
                     /transl_table=11
                     /translation="MSRISKVAAVMAGTGALIAGGAGLATADSGAEALAAHSPGVASGN
                     VVQVPVHIPVNVCGNTVNVIALLNPAFGNFCANVTDGHHEEGGHGH"
     gene            complement(4122..4982)
                     /locus_tag="ASD51_RS06980"
                     /old_locus_tag="ASD51_25460"
     CDS             complement(4122..4982)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157951.1"
                     /locus_tag="ASD51_RS06980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25460"
                     /product="tyrosinase family protein"
                     /protein_id="WP_056787931.1"
                     /transl_table=11
                     /translation="MYSRKDQRNLTRTEKKRLVDAILELKRSGRYDEFVTMHRQYYVTD
                     AEGRPRAAHMSPSFFPWHRRYLLEFEEALQAVDPTVSIPYWDWTRDNTPAASLWAEDFL
                     GGNGRSGDRQVMTGPFAYRQGNWRVSGGATDGRFLTRNFGRPSAPVDLPTAADVDRAMG
                     DPVYDAAPWNSLSTTGFRNRIEGWGISGTRRVANHNQVHLWIGGSMAGAGSPDDPVFWL
                     HHAYMDLLWARWQSAHPRSGYQPGSRLPDDDPQRDHVFAGDAPLPPWDETPSQLLDHTG
                     VYEYV"
     gene            complement(5001..5471)
                     /locus_tag="ASD51_RS06985"
                     /old_locus_tag="ASD51_25465"
     CDS             complement(5001..5471)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157950.1"
                     /locus_tag="ASD51_RS06985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25465"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_056787935.1"
                     /sec_met_domain="melC (E-value: 1.3e-47, bitscore: 155.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDPTAAASGEAAGGRGPVRRAVLRTAFTATVIAGTGGALAPVLLR
                     GPGAGAPEERGAPEPERFWERYRGREIRGTATVMVPAGGQSRAGEAASVAGVPDVEVRV
                     DGRPLHVMRRADGSYLSTVNHYESFPTLLATARAAVDEIGTSQLSTASPHTN"
     misc_feature    5661..5672
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            5665..6954
                     /locus_tag="ASD51_RS06990"
                     /old_locus_tag="ASD51_25470"
     CDS             5665..6954
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575908.1"
                     /locus_tag="ASD51_RS06990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25470"
                     /product="cytochrome P450"
                     /protein_id="WP_056787940.1"
                     /sec_met_domain="p450 (E-value: 4.8e-18, bitscore: 58.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAPTQVSTDRGRGTADADLADPSFWRLPRTERLDAFARLRELGP
                     PVRFTPRTGSRTPFYALVRYEDVKAASRQPQVFASAPGVTTPEPARWAKALFGNSMVNM
                     DGAEHAGLRRTISRAFTPRLLATAEENIDGIARRLVDELASGRSGDFMRSAPARMPFEV
                     ICDLMGIPRRHRAEIAAQIDHASEYVGVQRRGRARLRVPGRGLRSLAGLQIVMARVARE
                     RRRRPEEDLISALVRADVEGQALSSRQLGAFFSLLLVAGVETTRNAIAHGMVLLSAHPE
                     QRALLESDFDRYADGAVDEIVRHSTPIIQFRRTVAAEYALGGRVFLPGEKVMLLYASAN
                     RDASVFAGPDTFDITRSPNPHLGYGGGGPHHCLGAHLARLEMKALFRELLTRPERVRAV
                     GEPELVDSNFDNRVRSLPFTFGGIHPNGPA"
     gene            7066..8253
                     /locus_tag="ASD51_RS06995"
                     /old_locus_tag="ASD51_25475"
     CDS             7066..8253
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575909.1"
                     /locus_tag="ASD51_RS06995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25475"
                     /product="glycosyl hydrolase"
                     /protein_id="WP_056787945.1"
                     /transl_table=11
                     /translation="MPAQRRLVNFGIGTAVLGLLVGGAVVADRGAPDSMAGKGVSPAPG
                     GPEISTAVGAYLDYGPKGVQRMAELSRWLGGTEMRVGHSYLPGDLWVNIEGSPGFLGSW
                     AAWRRASEDRLFVLNTPMLERNEARVPDHEVRGLLRAGADGEFDEHFRRLADRLVVLGV
                     PDTVIVLGWEMNGTTYTHRCGPDPTAWKAYWRRIVTTMRSVPGQEFRFDFAPNRGRDAV
                     PWTECYPGDDVVDIIGMDSYDQPPARTFDDQVNEPYGLQKQVDFAAEHGKPISFPEWGL
                     FRNGDNPEYMRRMLEWIEQHEPVYQTITDYCPHGVWQCADNPESSRVYRDMLTRQPGAG
                     DPVLPAPVPIEPPKPEKPAPVVPEPDPNRREWCIGVPLSDWFGQWVPDQEVCLPG"
     gene            8708..9700
                     /gene="dhaK"
                     /locus_tag="ASD51_RS07000"
                     /old_locus_tag="ASD51_25480"
     CDS             8708..9700
                     /EC_number="2.7.1.121"
                     /GO_function="GO:0004371 - glycerone kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="dhaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514941.1"
                     /locus_tag="ASD51_RS07000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25480"
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="WP_056787949.1"
                     /transl_table=11
                     /translation="MKMLINVPETVVADALRGMAAAHPELTVDVENRVVVRRDAPVAGK
                     VGLVSGGGSGHEPLHAGFVGPGMLSAACPGEVFTSPVPDQMVRAAAAVDSGAGVLFIVK
                     NYTGDVLNFDMAVELAEDEGLRVARVLVDDDVAVSDSTFTAGRRGTGATLFVEKIAGAL
                     ADEGAPLERVEAVARRVNESSRSFGVALGAVTTPAKGSPTFDLSSGELELGIGIHGEPG
                     RERRPMMTSGEIADIAVHAVLEDLRPDGPVLVLVNGMGATPLLELYGFGAEVHRVLSER
                     GVAVARTLVGNYVTSLDMAGCSVTLCQVDEEMLRLWDAPVQTSALRWGR"
     misc_feature    complement(8709..8723)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.26"
                     /tool="antismash"
     gene            9740..10357
                     /gene="dhaL"
                     /locus_tag="ASD51_RS07005"
                     /old_locus_tag="ASD51_25485"
     CDS             9740..10357
                     /EC_number="2.7.1.121"
                     /GO_function="GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="dhaL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044270.1"
                     /locus_tag="ASD51_RS07005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_25485"
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="WP_056787952.1"
                     /transl_table=11
                     /translation="MLDTDFFRRWMAATAAAVDREASRLTELDAAIGDADHGSNMQRGF
                     TAVTAVLERESPATPGAVLTLAGRQLISTVGGASGPLYGTLLRRTGKALGDEPEVTDGR
                     FADALEAGVEAVAQLGGAKAGDKTMLDALLPAVGALRSSAGAARDAALAGALATVPLQA
                     RKGRASYLGERSIGHQDPGATSAALLVAALVEAAGTSGGGDA"
     misc_feature    complement(10406..10420)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 atccgtacgg catcaccgtc tccgccgaac gcctggacga gcagctgacc tggctgcgcc
       61 gccggggtct gaccgcggtg ggcgtcgccg ccctgctgcg cgggcgcgcc gccggccggc
      121 gcggactggt cggtctgacg ttcgacgacg ggtacgccga cttcctcgac gaggccctgc
      181 cggtgctggc ccggcacggc tgcacggcga cggtgttcgt cctgcccggc ctcccgagcg
      241 gctccaacgc gtgggatccg ctgggcccgc gcagaccgct gctcaccgag gacggcatcc
      301 gcgccgccgc cggggcgggc atggagatcg gctcgcacgg catgcgccac cgggatctca
      361 cggcgctgtc cgacgacgag ctgcgcgcgg agacgcacgg cagccgggag gcgatccgcc
      421 gtctcaccgg aaccgaaccg gcgggtttct gctaccccta cggcaccgcc gaccggcggg
      481 tgctcgcatc ggtgcgcgat gccgggtacg cgtacggctg cgccctcgcc cccggccccc
      541 tgctggggcc gttggccctg ccccgtacgc atgtcagcca tgccgaccgg ggcgcccgcc
      601 tccgcgcgaa ggaggtgcgg caccggctgc gccaccggac cgccgccgct cccgcgcgcc
      661 ggacgagggg cgaggcatga aagccctgca catcatcacc ggcctcggtg tcggcggcgc
      721 cgagcagcaa ctgcgcctgc tgctgcgtca tctgccggtg cgctgcgacg tcgtgaccct
      781 caccaacccg ggagcggtcg ccgaagggct gcgcgccgac ggcatccggg tgcggaacct
      841 cgggatgcgg ggcaaccggg acctcaccgc cgtgcccagg ctggcccggc tcatccgccg
      901 gggcgcgtac gacctcgtgc acacgcatct gtaccgggcc tgcgtctacg ggcggatcgc
      961 ggctcggatc gcggggacca ccgcggtggc gacggagcat tcgctgggcc gtgcggacat
     1021 cgagggaagg ccgctcaccc gcggcacccg ggccctctac ctgcgcaccg agcggatggg
     1081 agcggcgacg gtggccgtct ccggcaccgt cgcaggccgg ctgcgcgact ggggtgtacc
     1141 ggctgcccgc atccacaccg tggccaacgg aatcgatgtg gcggccttcc gctacgacgg
     1201 cgccggacgg cggaccaccc gtgcctcgct cggcgtcccc caggacgcct tcgtggtggg
     1261 gggcgtgggc cgtctcgtgc ccggcaaacg cttcgacctg ctgatccgtg ccgtcgcggc
     1321 cctccccggg gcctggctcg tactggcagg ggacgggccg gaggccggtg cgctgcgcgt
     1381 cctcgccgcc cggtcgggga tcgcggaccg cgtccggttc ctcggggagt gcggggcggg
     1441 caacgcctcc cccggcattc ccgaggcgct gtgggccatg gacaccttcg tctcggcgtc
     1501 gcgtgaggag accttcggcc tggccgtcgt cgaggcactg gcagccggtc tgcccgtcgt
     1561 ccacggggcc tgtcccgccg tcgacgacct gcccgccgac cgggctcccg gcaccacccg
     1621 catctcctcc ctcaccgatc ccgtcggtga gctgacgacc gcgctgcgcc accggatgga
     1681 gagcggcacc cgcagactgc cgcccccgcc ggccgcggac cactacgaca tcaggctcag
     1741 cagcgaacgt ctcatgaccg tctacgcaca cgccctcgac accgcccccc cgaccgagag
     1801 agcccactca tgaccgactc gcccgagccg aaaccggccg tccccggacg gctgcgcagg
     1861 ctccgccccg cttccgtacg gcccacttgg tggcccctgc ccgcctgcgt cctgctcgga
     1921 gcggcgtgcg ggctgtccta cgggctgctc gcccagcccg agtacgcgtc caccagttac
     1981 gccgtggtga ccgcgggcaa gggcatcgac ccggccgcgg cgctgggtta cgcacagtcg
     2041 tacggccggc tggccaccag cgacgcgacc ctcggctacg cccgtggcgc tgccggggag
     2101 ccggtccgcg aactgcgcac ccacgtacgg tccgagacct cacccgactc accgatgatc
     2161 gcgatcaccg gtacgtcggc cgacccgcgc cgggcggcgg acatcgccaa cgcggtgacc
     2221 gaggcagtgg tcgtcagcag cgggcacgtg gccaaggaca ccggggtgaa gctgatcaag
     2281 ttctcgcacg cggtgcgccc ggtcgcgccg gtgtccccct ccgtacccct cggcacggcc
     2341 gtcggcggga gcgccggcgg cctcgtcggc ggcctcgtcc tcctcgtccg gccccgtccg
     2401 gggcgccggg gcgtcaccgc ccaggtcccg ggtccgtccc cggcggcggc cgccccggcc
     2461 ggggaacggg agctcgtacg atgacctccg accgcgacgg ccgactcacc gtcaccctct
     2521 gccgggacct gcgggagttc tccgcgctcg ctgcggaatg ggacgcgctg caccgccgtt
     2581 gcccgaccgc gacgccgttc cagagccacg cgtggctcca ttcgtggtgg ctgtcctacg
     2641 gtgtgcccgg ccggctccgg atcgtcctgg cccggcgggg cgaacggctg gtcggtgcgg
     2701 ctcccctgat gctcgcgcac cgcccgatgc ccctcctggt accgatgggc ggtgcgatct
     2761 ccgacttctt cgacatcctc gtcgacggcg cgtgcacgca cgacgcggtc gcggccctgg
     2821 agcgtgggct ggaccgggcc gccgtgcaca cggtgatcga tctgcgggag gtgcggccgg
     2881 acgcctcggc ccagctgctg ttcgcgctct ggccgggcgc gcggagcagg ctcaccgatt
     2941 cgacctgcat ggagctgccc gccctgccga tcaccgcact catcgagcgg atgccgggcg
     3001 cacgtggcca acgggtccgc gccaagctgc gcaagaccga cgccgcgggt atcgagtgcc
     3061 gtgcggtgac cgggcaccgg gtggcggccg cggtcgacac cctgctgcgc ctgcacgcgc
     3121 tccagtggcg cggccgcggg gtcaacgccg aacacctgcg cccccgattc cgcgcccacc
     3181 tggtgcgctc cacccgtcgg atggtgcgcg acggcgaggc gtcgctccgc gagttcctgc
     3241 tcgacggcga agtggtggcc gccgatctgt cgctccggtc ggcgctgctg accggcggat
     3301 acctctacgg cgccgatccc cggctcaggg agaggaaggt ggacgtgtcc tcgatgctgt
     3361 tgcgtgaagt ggcggagcag gcggcgggca ccggatccgg tgcgctgagc ctgctccgcg
     3421 gctcggagcc gtacaagaac cactgggctc cggtggcggt cgtcaatcag cgcctgctgc
     3481 tggcccgttc ggtcctggag ccgctgctga ggctccgtga gtcgcaggtg gtggtgcggg
     3541 accgggccgc cgagacggtg aagacccgat ttcccgccgc gcgcgactgg cgcggccggc
     3601 tcagcggcct cccgtcctcg gggctgctgg cgatgggccg caactgacga agaattacga
     3661 cttttaagcc ggtccgttac cgtctgtcga tttccgcgtt gtcagagcac gtcggccgca
     3721 cgatctgcag ccggtttcaa acctcccaag gagaactgat gtcgcgtatc tcgaaggtgg
     3781 ccgccgtcat ggcaggtacc ggtgccctga tcgccggcgg tgccggcctg gctaccgccg
     3841 actccggagc cgaggccctc gcggcccact cccccggtgt cgcatcgggc aacgtcgtgc
     3901 aggtgcccgt ccacatcccg gtcaacgtct gcggcaacac cgtgaacgtc atcgccctgc
     3961 tgaacccggc cttcggcaac ttctgcgcga acgtgacgga cggccaccac gaggagggtg
     4021 gccacggcca ctgatctcgt aggcgtccgg cccgggcctc ccctcctcgc tcccgcgaag
     4081 aggggaggcc cgcccgcttc ccgggcgggc ccgcctccgg ctcagacgta ctcgtagacg
     4141 ccggtgtggt ccaggagttg cgagggcgtc tcgtcccatg ggggcagtgg cgcgtccccg
     4201 gcgaatacgt ggtcgcgctg cgggtcgtcg tcgggaagcc tgctgcccgg ctggtagccg
     4261 gaccgagggt gcgccgactg ccagcgcgcc cagagcagat ccatgtaggc gtggtgcagc
     4321 cagaagaccg ggtcgtcggg cgagcccgcg ccggccatcg aaccgccgat ccagaggtgc
     4381 acctggttgt ggttggcgac cctgcgggtc ccgctgatgc cccagccctc gatcctgttc
     4441 cggaagccgg tggtgctgag gctgttccag ggcgcggcat cgtagaccgg gtcgcccatg
     4501 gcgcggtcca cgtccgccgc ggtgggcagg tcgaccggtg cggacggccg gccgaagttc
     4561 cgggtcagga accggccgtc ggtggctccc ccgctgacgc gccagttgcc ctgccggtag
     4621 gcgaacggcc ccgtcatgac ctggcggtcg ccggacctgc cgttgcctcc gaggaagtcc
     4681 tccgcccaga gggacgccgc cggggtgttg tcccgtgtcc agtcccagta cgggatcgac
     4741 accgtggggt cgacggcctg cagcgcctcc tcgaactcca gcaggtacct gcggtgccac
     4801 gggaagaagg acggtgacat gtgtgcggcc cgcggccggc cttcggcatc ggtgacgtag
     4861 tactgccggt gcatcgtgac gaactcgtcg tagcgtccgg agcgtttgag ctccaggatc
     4921 gcgtcgacca ggcgcttctt ctccgtgcgt gtgaggttgc gctgatcctt gcggctgtac
     4981 acccgtgctg tcctttgggt tcagttggtg tgcggggagg ctgtggagag ctgggaggtg
     5041 ccgatctcgt cgacggccgc gcgggccgtc gccagcagcg tcgggaacga ctcgtagtgg
     5101 ttcaccgtgc tgaggtagct gccgtcggcg cgtctcatca cgtgcagggg tctgccgtcg
     5161 acgcggacct cgacgtccgg cacgccggcc acggatgccg cctcaccggc acgtgactgc
     5221 ccgccggcgg gcaccatgac cgtggccgtg ccgcggatct cccggccgcg gtacctctcc
     5281 cagaaccgct cgggctccgg tgcgccgcgc tcctcgggcg ctccggcccc cgggcctcgg
     5341 aggaggaccg gggcgagcgc gccgccggta ccggcgatca ccgtcgcggt gaatgcggtg
     5401 cgcagcaccg cgcggcgtac cggtccgcgg cctcccgctg cttccccgct cgcagctgcg
     5461 gtcgggtcca tggggtgtac ctgcctctcg ttcgctcttc cgaggcgtct tcgctctcga
     5521 agtaggcaac gagatggccc acgaacggtt ctcgtcaggc acccatcagg cggccgtggg
     5581 aattctcggg gccccccggc cgcgtcggcc gcgcagcggc acacggcggg ccagcatggc
     5641 cgtccgaact cccggaggtt gtgcatgaac gcgccgacgc aggtttccac ggaccgcgga
     5701 cgagggacgg cggacgccga tctggccgac ccgtcgttct ggcggctgcc caggaccgag
     5761 cggctggacg cgttcgcccg gctgcgggag ctcggcccac cggtccgttt caccccgcgt
     5821 acgggctcac gcacgccgtt ctacgcactg gtgcggtacg aggacgtgaa ggcggcgagc
     5881 cggcagccgc aggtcttcgc cagtgcgccc ggggtcacca ccccggagcc ggcccgctgg
     5941 gcaaaggcgc tgttcggcaa ttccatggtg aacatggacg gcgccgagca cgccggcctg
     6001 cgcaggacca tctcccgcgc cttcaccccg cggctgctcg ccaccgccga ggagaacatc
     6061 gacgggatcg cccggcggct ggtggacgag ttggcctccg gacgctccgg cgacttcatg
     6121 cggtcggctc ccgcccgtat gcccttcgag gtcatctgcg acctgatggg cattccccgc
     6181 cgccaccgtg ccgagatcgc cgcgcagatc gaccacgcgt cggagtacgt cggggtccag
     6241 cgccgggggc gggcgcggct gcgggtaccc ggacgtggtc tgcgctccct ggccggcctg
     6301 cagatcgtga tggcgcgggt ggcccgggag cgtcgccgcc gtccggagga ggacctgatc
     6361 tcggcactgg tgcgtgcgga cgtcgagggg caggcactgt cctcccggca gctcggcgcg
     6421 ttcttctcgc tgctgctcgt ggcgggtgtg gagaccaccc gcaacgcgat cgcccacggg
     6481 atggtcctgc tgtccgccca cccggagcag cgcgccctcc tggaatccga cttcgaccgg
     6541 tacgccgacg gcgcggtgga cgagatcgtc cgccactcga caccgatcat ccagttccgc
     6601 cggacggtcg ccgccgagta cgcgctcggc ggccgcgtct tcctccccgg cgagaaggtg
     6661 atgcttctct atgcctcggc gaaccgggac gcgtcggtct tcgccggccc cgacaccttc
     6721 gacatcaccc ggtcgcccaa tccccacctg gggtacggcg ggggcggtcc ccatcactgt
     6781 ctgggcgcgc acctggcccg actggagatg aaagcactct tccgggaact cctcacccgc
     6841 cccgagcgcg tgcgagcggt gggggaaccg gagctggtcg actcgaattt cgacaaccgg
     6901 gtccgctccc ttcccttcac cttcggcgga attcacccga acggcccggc ctgaatcccc
     6961 ggccgtcgcg aatggtctat tcacggacgg tcgcagagcc cgttgttgaa catttctctt
     7021 tatggtcgac agggtcggag gtacgccaca acccatggag gatccgtgcc cgcacagcgc
     7081 cgcctcgtca acttcggaat cggtacggct gtgctcggcc tcctcgtcgg cggtgcggtc
     7141 gtcgcggacc ggggcgcccc ggactccatg gccggcaagg gcgtctcacc ggctcctggg
     7201 ggcccggaga tctcgaccgc cgtcggcgcc tacctggact acgggccgaa gggcgttcag
     7261 cggatggcgg agctgtcgcg ttggctgggc gggacggaga tgcgggtcgg gcacagctat
     7321 ctgcccggcg acctgtgggt caacatcgag ggcagtcccg gattcctcgg ctcgtgggcg
     7381 gcctggcggc gggcttcgga ggaccggctg ttcgtgctca acacgccgat gctggaacgc
     7441 aacgaggcac gcgtccccga ccacgaggtg cgcgggctgc tgcgggcggg ggccgacggg
     7501 gagttcgacg agcacttccg caggctcgcg gaccggctgg tcgtgctggg cgtcccggac
     7561 acggtgatcg tgctcggctg ggagatgaac gggaccacgt acacccaccg ctgcggtccc
     7621 gatcccaccg cgtggaaggc gtactggagg cgcatcgtca ccacgatgcg gtcggtgccc
     7681 ggtcaggagt tccgcttcga cttcgctccg aaccggggac gggacgccgt tccctggacc
     7741 gagtgctatc ccggcgacga cgtcgtggac atcatcggca tggactcgta cgaccagcct
     7801 cccgcccgta cgttcgacga tcaggtgaat gagccctacg gcctgcagaa gcaggtcgac
     7861 ttcgccgcgg agcacggcaa gccgatctcg ttccccgagt gggggctgtt ccgcaacggc
     7921 gacaatcccg agtacatgcg ccgcatgctg gagtggatcg aacagcacga gccggtgtac
     7981 cagacgatca cggactactg cccgcacggc gtgtggcagt gcgccgacaa ccccgagtcc
     8041 tcccgggtgt accgcgacat gctgacccgg cagccgggtg ccggggaccc cgtcctgccg
     8101 gcacccgtgc cgatcgagcc cccgaagccg gagaagcccg cacccgtcgt gcccgagccg
     8161 gacccgaaca ggcgcgagtg gtgcatcggg gttccgttga gcgactggtt cggccagtgg
     8221 gtgcctgacc aggaagtctg tctgcccggt tgaggcgccg ctcgccgact cgcggccgct
     8281 gcccctgccg gctcccgggc agcggccccc atggcgtggg cggggtgccc gcggggctgg
     8341 gcccgctcgc cctgctcgga cgattcgtcg aacgcgcctc cactccggcg gcttcgtccc
     8401 cgtcacgccc cggggaccgg ccacccggct tcgggtgacg cggggaggtg gagtgccggc
     8461 ggttccgccc atccgatgcg ccgcgccgct tccccgatca cgcgcgtggt gttgatactg
     8521 gtgtccgcac cgacgccgac cggagggggc actcctggcg gagagccggg gtcggtgggc
     8581 gccggcccgg cgtcaccccc cgcccggtcc gccgccggcc accaccgggg aacgcaatcc
     8641 cacccacacc cgccgacccg gcagcaccct gcaccgggca cggcgggtac gtacggagga
     8701 gttaccgttg aagatgctga tcaacgtgcc ggagaccgtg gtcgcggacg ccctccgcgg
     8761 tatggccgcc gcccaccccg agctcaccgt ggacgtcgag aaccgggtgg tcgtgcggcg
     8821 cgacgcaccg gtggccggga aggtcgggct cgtgtccggc ggcggatccg ggcacgagcc
     8881 gctgcatgcg ggcttcgtcg ggcccggcat gctctcggcc gcgtgtcccg gtgaggtctt
     8941 cacctcgccc gtgcccgacc agatggtccg ggccgccgcc gccgtcgaca gtggcgcggg
     9001 ggtgctgttc atcgtcaaga actacaccgg tgacgtgctg aacttcgaca tggcggtcga
     9061 actcgccgag gacgagggcc tgcgggtcgc ccgggtcctg gtcgacgacg acgtggcggt
     9121 gtccgacagc acgttcacgg cgggccgccg gggcacgggc gcgacgctgt tcgtcgagaa
     9181 gatcgcgggt gcgctcgccg acgagggtgc accgctggag cgggtggagg ccgtggcgcg
     9241 ccgggtcaac gagtcctcgc gcagtttcgg ggtggcgctc ggtgccgtca ccacgccggc
     9301 gaagggcagc ccgacattcg atctctcttc gggcgaactg gagttgggca tcggtatcca
     9361 cggcgagccc gggcgggagc gccgtccgat gatgacgtcc ggagagatcg ccgacatcgc
     9421 ggtccacgcg gtgctggagg atctgcggcc ggacggcccg gtcctggtgc tggtcaacgg
     9481 gatgggcgcg acgccgctgc tggagctgta cgggttcggt gccgaggtgc accgggtgct
     9541 gtccgagcgg ggggtcgcgg tggctcgtac gctggtgggg aactacgtga cgtcgctcga
     9601 catggcgggc tgttcggtga cgctctgcca ggtcgacgag gagatgctcc ggctctggga
     9661 cgcaccggtg cagacgtccg cgctccgctg gggccggtga accggcccgc cgcccgatgt
     9721 gtgaggaaca ggagatgacg tgctcgacac cgatttcttc cgccgctgga tggccgccac
     9781 cgccgcggcc gtcgaccgtg aggcgagccg gctgaccgaa ctggacgccg cgatcgggga
     9841 cgccgatcac gggagcaaca tgcagcgcgg attcacggcg gtgacggccg tcctggagag
     9901 ggagtccccc gcgacgcccg gcgcggtgct gacgctcgcg ggccggcagc tgatctcgac
     9961 ggtgggcggg gcgtcgggac cgttgtacgg aacgctgctg cgccgcaccg gcaaggcact
    10021 tggggacgaa cccgaggtga cggacggccg gttcgccgac gcactcgagg ccggcgtcga
    10081 ggccgtcgcg cagttgggcg gggcgaaggc cggggacaag accatgctcg acgctctgct
    10141 gccggcggtc ggggcgctga ggtcctccgc cggggcggcc cgggacgcgg cgctggccgg
    10201 tgcgctggcg acggtgcccc tgcaggcgcg caagggcagg gccagttatc tgggcgagcg
    10261 cagcatcggg caccaggatc cgggggcgac ctccgccgcg ttgctggtcg cggccctcgt
    10321 cgaggcggcg gggacgagcg gtggtgggga cgcgtgagcg aggagcagca ggtcgggatc
    10381 gtgctggtgt cgcacagcgg cccggtggcc gagaccgtcg ccgaactggc gcgaggactg
    10441 gcggccggcg gggcgaccgc acccgtggcc g
//
