LOCUS       NZ_LMFT01000001         5419 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root55 contig_1, whole genome shotgun sequence.
ACCESSION   NZ_LMFT01000001
VERSION     NZ_LMFT01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root55
  ORGANISM  Streptomyces sp. Root55
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 917440)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 917440)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140605
            Assembly Name                     :: Root55
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001427245.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 01:50:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,084
            CDSs (total)                      :: 7,008
            Genes (coding)                    :: 6,730
            CDSs (with protein)               :: 6,730
            Genes (RNA)                       :: 76
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 105 of 278
            Pseudo Genes (incomplete)         :: 225 of 278
            Pseudo Genes (internal stop)      :: 24 of 278
            Pseudo Genes (multiple problems)  :: 67 of 278
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:11:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5419
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMFT01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..5419
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..5419
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5419
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[20:419](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      21..419
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            21..419
                     /locus_tag="ASD51_RS00005"
                     /old_locus_tag="ASD51_00005"
     CDS             21..419
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="ASD51_RS00005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_00005"
                     /product="ectoine synthase"
                     /protein_id="WP_056785815.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.4e-54, bitscore:
                     178.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWISPGTMYLLNGHEHHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     misc_feature    complement(1020..1036)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.54"
                     /tool="antismash"
     assembly_gap    1307
                     /estimated_length=1
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1308..1418
                     /locus_tag="ASD51_RS35995"
                     /pseudo=""
     CDS             <1308..1418
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="ASD51_RS35995"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /pseudo=""
                     /transl_table=11
                     /translation="NVFIVFNSVENTAQEPFAAPIRRPEFIGARDFTPVK"
     misc_feature    1660..1678
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.32"
                     /tool="antismash"
     gene            1661..2359
                     /locus_tag="ASD51_RS00015"
                     /old_locus_tag="ASD51_00015"
     CDS             1661..2359
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987768.1"
                     /locus_tag="ASD51_RS00015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_00015"
                     /product="hypothetical protein"
                     /protein_id="WP_056785821.1"
                     /transl_table=11
                     /translation="MRTVRRTTALVLTCLLAGAALAGCGGGADDADDKSAADLLDEANA
                     TMGELSSLTIEITNEAGDDTATWRMTTDLKSRCQVRNTFSGSGTLEQIRIGGTDYVRPD
                     TKYLETWSGNDLGAARPNVWAKVPVARSKPGDGLSRCTRPFESFGTATKGEATRIGGRE
                     ALGLEVTDPADKEGAYTFYVATEGEPHLLKAVYDGGKQVTTTSFSDFGEPLDIRPPASA
                     DVLDMSDVTG"
     gene            complement(2390..2620)
                     /locus_tag="ASD51_RS00020"
                     /old_locus_tag="ASD51_00020"
     CDS             complement(2390..2620)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASD51_RS00020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ASD51_00020"
                     /product="superinfection immunity protein"
                     /protein_id="WP_056783425.1"
                     /transl_table=11
                     /translation="MISTIGPLELLVIVPLALLILCVPTFIAHRRRVDRLGLVVVVSVI
                     GAATGLFWFVALVMALSMRTRGPAPVPGMSG"
     gene            2750..3757
                     /locus_tag="ASD51_RS00025"
                     /old_locus_tag="ASD51_00025"
     CDS             2750..3757
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156965.1"
                     /locus_tag="ASD51_RS00025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_00025"
                     /product="VOC family protein"
                     /protein_id="WP_056783428.1"
                     /transl_table=11
                     /translation="MTSLVQHITIDCADAYELALFWAEVLGSSLSDDDRPGDPEALVAA
                     PGAALLFVTVPESKSVKNRIHLDLRPEDRGRDAEVERLLALGATLVGDHRRPDGRGWAT
                     MADPEGNEFCVECGAAERAALNGTRMPVTADDVALAVRLAVDTLGESEARDWRVPAGSL
                     EWNCWETVEHLGDDLFAYAVQLGLRKPPTGTEVPYRWAAEREGGPANSVFADPAAGSTG
                     LLQTLEASGSLLTAMVRTALPDVRSHHVFGLSDPEGFAAMGVVETLVHTHDVAEGLSLS
                     WTPPADLCDRALARLFPDAPDDADRWTVLLWSTGRADLPGRDRVTSWRWHGAPL"
     gene            complement(3761..5320)
                     /locus_tag="ASD51_RS00030"
                     /old_locus_tag="ASD51_00030"
     CDS             complement(3761..5320)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576697.1"
                     /locus_tag="ASD51_RS00030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD51_00030"
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="WP_056783431.1"
                     /transl_table=11
                     /translation="MYGTTHTRRRAAGTVAALSGLLFAALPAQAAHAATTPANPGFESG
                     ATGWSTYSAGGTNAASFTEAGGHAGSTRLSHWSASAYKVETYQYLTGLTDGTYTLSAWV
                     RSGGGQNSAYIALRNCGAAEQRTDLPPTADGGWIRLVTSVKVTGGACTISLNSDAHAGQ
                     WANFDDITFTPGTTGLSVRGGDLSTLPKNEAHGATYRDANGTAGDAVTILRNSGMNYVR
                     LKVWVNPADGYNNKPRVLAMAKRAKALGMKTLIDFHYSDAWADPGKQNKPAAWAGHSYT
                     QLRTDVYNHTYDVLNALKAQGTTADMVQIGNETNGGMLWPEGSTDNWTQLAGLLTSGAN
                     AAKAVSSGTKVALHLAEGGDNAGTRWWFDNAVSRGVPFDVVALSYYGYWHGSLSELQTN
                     LDDAASRYGKPVLVAETAYAHTLADDDGLENNVATADQLVTGYPATPAGQAANLRDVMN
                     VVEAVPNGRGLGAVYWEPAWTAVTGSGWDPADPASGNAWENQAVFDYNGKLLPAARWFS
                     HR"
ORIGIN
        1 gagaaaggca ccatcccacc gtgatcgtcc gttcgttcag tgacatcgag aacaccgacc
       61 ggcatgtgaa ggccgcctcc ggaacctggg agagcaagcg catcgtgctc gccaaggaga
      121 aggtcggctt ctcgctccac gagaccgtgc tctacgccgg cacggagacg tccatgtggt
      181 acgcgaacca catcgaagcc gtgctgtgca ccgagggaga ggccgagctc accaacgacg
      241 agaccggcga gacacactgg atctcccccg gnacgatgta cctgctgaac ggccacgaac
      301 accacaccct gcggcccaag accgacttcc gctgcgtgtg cgtcttcaac ccccccgtca
      361 ccggacggga ggaccacgac gagaacggtg tctacccact gctgacagag gaggcctgaa
      421 ccatgaccac cgatgtacga gccgacctgt acccctcgcg cggcgccgcc gagatgacca
      481 cnccccgcca ggacccggtc atctggtccg cgcccggcgc acccgggccg ctctccgcga
      541 aggacctcca gggcttcgag cacgacggct tcctcaccgt cgaccagctc atctccccgc
      601 aggaggtggc cggctaccac gcggagctgg aacggctgat cgccgatccg cgggtccggg
      661 ccgacgaacg ctcgatcatc gagccgacgt cgcagagcgt gcggtccgtc ttcgaggtcc
      721 accggctcag cgaggccttc gcccgcctcg tcgccgacga acgcgtcgtg ggccgggccc
      781 gccagatcct cggctccgac gtctacgtcc accagtcccg catcaacgtc aagcccggct
      841 tcggcgcctc cggnttctac tggcactccg acttcgagac ctggcacgcc gaggacggcc
      901 tgccgaacat gcggaccgtg tccgtctcca tcgcgctgac cgagaaccac gacaccaacg
      961 gcgggctcat gatcatgccc ggctcccaca agtcgttcct cggctgctcc ggcgagacac
     1021 cgaaggacaa ctacaagaaa tccctgcaga tgcaggacgc cggcacnccc tccgacgagg
     1081 cnctgacgaa gatggccgac cgccacggca tcaagctctt cacgggcaaa gccggatcgg
     1141 ccacctggtt cgactgcaac gccatgcacg gctccgggga caacatcacc ccctacccgc
     1201 gcagcaacgt cttcatcgtc ttcaacagcg tcgagaacac ggcacaggaa cccttcgcag
     1261 cccccatccg ccgccccgaa ttcatcggag cacgcgactt caccccnaac gtcttcatcg
     1321 tcttcaacag cgtcgagaac acggcacagg aacccttcgc agcccccatc cgccgccccg
     1381 aattcatcgg agcacgcgac ttcaccccgg tgaagtaggg gcgtacgacg gttgaagggg
     1441 cgggacgcac agcgcgtccc gccccttcgg cgtcatcatc ccgtgcggta tcgggcggag
     1501 cacgccggac ggtccgtcgc actcccgctc cccgacggcc ggaacgcacg ctcgtgtcct
     1561 gtgccggatc cacgcccgac cgggccgcca ggaggccgtt ggtaggctca tgtggtcgat
     1621 caaggtcgtg cccgagtgcg cgccggagag gtggcaccac atgcggacgg ttcgcaggac
     1681 gacggcactg gtactgacgt gcctgcttgc gggggccgcg ctcgccgggt gcgggggcgg
     1741 tgccgacgac gccgacgaca agtcggcggc ggacctcctc gacgaggcca acgccacgat
     1801 gggcgagctg agttccctca ccatcgagat caccaacgag gcgggcgacg acacggcgac
     1861 gtggcggatg acgaccgatc tgaagagtcg ctgccaggtg aggaacacct tctccggaag
     1921 cggcaccctg gaacagatcc ggatcggcgg gacggactac gtccgcccgg acacgaagta
     1981 cctggagacc tggagcggca acgatctcgg tgccgccagg ccgaacgtct gggcgaaggt
     2041 gcccgtcgcc aggtcgaagc cgggtgacgg gctgtcccgg tgcacccggc cgttcgagtc
     2101 gttcggtacg gcgacgaagg gcgaggccac ccgcatcggc ggacgggagg cgctcgggct
     2161 ggaggtgacg gatccggcgg acaaggaggg ggcgtacacg ttctacgtcg ccaccgaggg
     2221 cgagccgcat ctgctgaagg ccgtgtacga cggcgggaag caggtcacca ccacctcgtt
     2281 cagcgatttc ggcgaaccgc tggacatccg gccgcccgcg tcggccgacg tgctggacat
     2341 gagcgacgtc acgggctagt gccgccgccc ggggccaccg gacaccgggt cacccgctca
     2401 tgccgggcac cggcgcgggg ccgcgggtgc gcatcgacag cgccatgacc agggcgacga
     2461 accagaacag gcccgtggcg gcgccgatca cgctgaccac gacgaccagt cccaggcggt
     2521 ccaccctcct gcggtgggcg atgaacgtcg gcacgcagag gatgaggagt gccaacggca
     2581 cgatgacgag gagctccagg ggacctatgg tcgagatcat cgggtcactg taccgatatg
     2641 acggcccgtc ttataggttc tcagagcccg ctgcccggag cagcgggctc cacttgtccg
     2701 gattcaagtc gctggacctc ggcgtgggtc agcggcgaca gtgtcgccca tgacatcgct
     2761 cgtacagcac atcacgatcg actgcgccga cgcctacgaa ctggccctct tctgggccga
     2821 ggtgctcggc tcgtcgctgt ccgacgacga ccgtcccggt gatcccgagg cgctcgtcgc
     2881 ggccccgggg gcagcgctgc tgttcgtcac cgtgcccgag tccaagagcg tcaagaaccg
     2941 gatccacctc gacctccggc ccgaggaccg cggccgcgac gccgaggtcg aacggctgct
     3001 cgctctcggc gccacgctgg tcggtgacca ccgcaggccc gacgggcggg gctgggcgac
     3061 gatggcggat cccgagggca acgagttctg tgtggagtgc ggcgccgccg aacgggcggc
     3121 gctgaacggg accaggatgc cggtcaccgc ggacgacgtg gccctcgcgg tccgtctcgc
     3181 ggtcgacacc ctcggggagt cggaggcgcg ggactggcgg gtgccggccg ggtcgttgga
     3241 gtggaactgc tgggagaccg tcgagcatct cggcgacgac ctgttcgcgt acgcggtcca
     3301 gctcggcctc cggaagccgc cgaccggcac cgaggtcccc taccggtggg cagcggagcg
     3361 cgaggggggc ccggcgaatt cggtcttcgc ggaccctgcc gccgggtcca ccgggctgct
     3421 gcagaccctg gaggcgagcg gttccctgct cacggcgatg gtgcgcacgg ccctgccgga
     3481 cgtccgctcg caccatgtgt tcgggctctc cgacccggag gggttcgccg cgatgggcgt
     3541 cgtggagacc ctggtgcaca cccacgacgt cgccgaaggg ctgtccctct cctggacgcc
     3601 gcccgcagac ctgtgcgacc gggcgctggc acggctcttc ccggacgcgc cggacgacgc
     3661 ggaccggtgg accgtcctgc tctggtccac cggccgagcc gatctgccgg gccgtgaccg
     3721 ggtcacctcg tggaggtggc acggtgctcc gctgtagtgg tcaccggtgc gagaaccacc
     3781 gtgccgcggg cagcagcttc ccgttgtagt cgaacaccgc ctggttctcc cacgcgttgc
     3841 ccgacgccgg gtcggccggg tcccagccgc ttccggtgac ggcggtccag gccggctccc
     3901 agtagacggc gccgagtccg cggccgttcg ggacggcctc gacgacgttc atgacgtccc
     3961 ggaggttggc cgcctgcccg gccggggtcg ccgggtagcc ggtgaccagc tggtccgcgg
     4021 tcgccacgtt gttctccagg ccgtcgtcgt ccgcgagcgt gtgggcgtac gcggtctcgg
     4081 cgaccagcac cggcttcccg taacgggacg cggcgtcgtc caggttggtc tggagctcgg
     4141 acagcgagcc gtgccagtag ccgtagtagg acagggcgac gacgtcgaag gggacgccgc
     4201 gcgacacggc gttgtcgaac caccagcggg tgcccgcgtt gtcgccgccc tcggcgaggt
     4261 gcagcgccac cttcgtaccg gaggagacgg ccttggcggc gttcgccccc gaggtcagga
     4321 ggcctgccag ctgggtccag ttgtcggtgg acccttcggg ccagagcatt ccgccgttgg
     4381 tctcgttgcc gatctgcacc atgtcggcgg tggtgccctg ggccttgagc gcgttcagca
     4441 cgtcgtacgt gtggttgtag acgtccgtgc ggagctgcgt gtagctgtgg ccggcccagg
     4501 ccgcgggctt gttctgctta ccggggtcgg cccaggcgtc ggagtagtgg aagtcgatga
     4561 gcgtcttcat gccgagggcc ttggcgcgct tggccatggc gaggacgcgg ggcttgttgt
     4621 tgtagccgtc ggccgggttc acccagacct tgaggcggac gtagttcatc ccggagttcc
     4681 tgaggatggt caccgcgtcc ccggcggtgc cgttcgcgtc gcggtacgtc gcgccgtggg
     4741 cctcgttctt cgggagggtg gagaggtccc cgcccctgac gctcaggccc gtggtcccgg
     4801 gggtgaaggt gatgtcgtcg aagttggccc actgccccgc gtgcgcgtcg gagttgaggc
     4861 tgatcgtgca cgcgccgccg gtcaccttga cggaggtgac gagccggatc cagccgccgt
     4921 cggcggtggg cggcaggtcg gtgcgctgct cggcggcgcc gcagttgcgc agggcgatgt
     4981 aggcggagtt ctggccgccg cccgaacgca cccaggcgct gagggtgtac gtcccgtcgg
     5041 tgaggccggt gaggtactgg taggtctcga ccttgtacgc cgaggccgac cagtggctga
     5101 gcctggtgct gcccgcgtgg ccgcccgcct cggtgaagga ggcggcgttc gtgcctccgg
     5161 ccgagtacgt cgaccagccg gtggccccgg actcgaagcc tgggttcgcg ggggtggtgg
     5221 ccgcgtgggc ggcctgtgcg gggagtgcgg cgaacagcag gccggacagg gcggccaccg
     5281 ttcctgcggc tcttctgcgg gtgtgcgtcg ttccgtacat cgtcgatgtc ccttcgaccg
     5341 ttgtgcgggg taacggaggt ggtgcggggg tgcgtcggtg cgcctcagcc gtcgagccgt
     5401 acgacgcgca ccgcgcccg
//
