LOCUS       NZ_LMEU01000022         6286 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces sp. Root1319 contig_29, whole genome shotgun sequence.
ACCESSION   NZ_LMEU01000022
VERSION     NZ_LMEU01000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root1319
  ORGANISM  Streptomyces sp. Root1319
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 59455)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 59455)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140604
            Assembly Name                     :: Root1319
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426615.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 02:19:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,915
            CDSs (total)                      :: 5,857
            Genes (coding)                    :: 5,667
            CDSs (with protein)               :: 5,667
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 1 (16S, 23S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 64 of 190
            Pseudo Genes (incomplete)         :: 160 of 190
            Pseudo Genes (internal stop)      :: 8 of 190
            Pseudo Genes (multiple problems)  :: 38 of 190
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:44:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6286
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEU01000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..6286
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..6286
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6286
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[887:1286](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(888..1286)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(<1..884)
                     /gene="thpD"
                     /locus_tag="ASD26_RS12420"
                     /old_locus_tag="ASD26_28210"
     CDS             complement(<1..884)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="ASD26_RS12420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD26_28210"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_056785818.1"
                     /sec_met_domain="PhyH (E-value: 3.8e-41, bitscore: 133.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTDVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPLSAKDLQGF
                     EHDGFLTVDQLISPQEVAGYHAELERLIADPRVRADERSIIEPTSQSVRSVFEVHRLSE
                     AFARLVADERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENHDTNGGLMIMPGSHKSFLGCSGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENTAQEPFAAP
                     IRRPEFIGARDFTP"
     gene            complement(888..1286)
                     /locus_tag="ASD26_RS12425"
                     /old_locus_tag="ASD26_28215"
     CDS             complement(888..1286)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="ASD26_RS12425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD26_28215"
                     /product="ectoine synthase"
                     /protein_id="WP_056785815.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.8e-55, bitscore:
                     178.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWISPGTMYLLNGHEHHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            complement(1349..2611)
                     /gene="ectB"
                     /locus_tag="ASD26_RS12430"
                     /old_locus_tag="ASD26_28220"
     CDS             complement(1349..2611)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 1.9e-71.
                     Score: 232.7. Matches aSDomain:
                     nrpspksdomains_ASD26_RS12430_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="ASD26_RS12430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD26_28220"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_057573609.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.3e-78, bitscore:
                     256.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFESLESEVRSYCRSWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFENVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFEGKVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQDLCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKGELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQMEKQTLARGEQVEQTLL
                     AICAEEPTAQFRGRGLVWGMEFTDPARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARSVRETA"
     aSDomain        complement(1523..2527)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD26_RS12430_Aminotran_3.1"
                     /evalue="1.90E-71"
                     /label="ASD26_RS12430_Aminotran_3.1"
                     /locus_tag="ASD26_RS12430"
                     /protein_end="363"
                     /protein_start="28"
                     /score="232.7"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFENVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFEGKVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQDLCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKGELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGQMEKQTLARGEQVEQTLLAICAEEPTAQFRGRGLVWGMEFT"
     gene            complement(2689..3219)
                     /gene="ectA"
                     /locus_tag="ASD26_RS12435"
                     /old_locus_tag="ASD26_28225"
     CDS             complement(2689..3219)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607778.1"
                     /locus_tag="ASD26_RS12435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD26_28225"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_245376832.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDTPRVEDGAAIWRIARDSEVLDLNSSY
                     SYLLWCRDFAATSVVARDETGGPIGFVTGYLRPDRPGTLVVWQVAVDSAHRGKGLAATL
                     LDALTTRVASDQGLASVETTVTPDNTASDRLFTSFAQRHDVPLEREVLFDGELFPEGTH
                     LPEVLYRIGPFHA"
     misc_feature    3331..3346
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.25"
                     /tool="antismash"
     misc_feature    complement(3374..3389)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    3384..3399
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     gene            complement(3552..4658)
                     /locus_tag="ASD26_RS12440"
                     /old_locus_tag="ASD26_28230"
     CDS             complement(3552..4658)
                     /NRPS_PKS="Domain: Aminotran_5 (74-308). E-value: 3.5e-27.
                     Score: 87.2. Matches aSDomain:
                     nrpspksdomains_ASD26_RS12440_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518886.1"
                     /locus_tag="ASD26_RS12440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD26_28230"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_056792699.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.4e-22, bitscore:
                     72.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLTAAHFAAIERRVAGLLATGQDVVIMQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGAEVVDLAVPFHSAVTADQVAQALAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDVWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWERMAGNPQAPRHSYLSLLDWKARWIDGGRKALPHAP
                     AQLEMLALEACVERIEAEGLDTLTARHAAAAAATRAGAVALGAGLEPYVHDARDAAPVA
                     TTLRVPAGTDASALVAKALAGDPSLPLIAGGGALSTEMIRVNHYGVDATRAAVHASLAA
                     LGAALAETGREADFEGARNAVSETWPND"
     aSDomain        complement(3735..4436)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD26_RS12440_Aminotran_5.1"
                     /evalue="3.50E-27"
                     /label="ASD26_RS12440_Aminotran_5.1"
                     /locus_tag="ASD26_RS12440"
                     /protein_end="308"
                     /protein_start="74"
                     /score="87.2"
                     /tool="antismash"
                     /translation="RDCGAEVVDLAVPFHSAVTADQVAQALAEHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDVWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWERMAGNPQAPRHSYLSLLDWKARWIDGGRKALPHAPAQLEMLALEACVERI
                     EAEGLDTLTARHAAAAAATRAGAVALGAGLEPYVHDARDAAPVATTLRVPAGTDASALV
                     AKALAGDPSLPL"
     gene            4763..5863
                     /locus_tag="ASD26_RS12445"
                     /old_locus_tag="ASD26_28235"
     CDS             4763..5863
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="ASD26_RS12445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD26_28235"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_079099400.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.3e-20, bitscore:
                     64.1, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDHEVARVLRVEGRVLAGPDDVRDGLWAVGGRITYERPPGADDA
                     VTLTGWSLPGLVDAHCHVGLDRHGPVDAATSEKQALTDRAAGTLLIRDAGSPSDTRWID
                     DREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREAERGDGWVKLVGDWIDRD
                     AGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEAALRDLVEAGIDCIEHATGLTEDTI
                     PLFAERGVAIVPTLVNIATFPDLAAGGEAKFPRWSAHMRQLYERRYDTVRAAYDAGIPV
                     FVGTDAGGSLAHGLVAAEVAELVKAGIPPLEALSATAWGARRWLGRPVLEEGAPADLVV
                     YDEDPRADVRVLAAPRWVVLDGRVVG"
ORIGIN
        1 ggggtgaagt cgcgtgctcc gatgaattcg gggcggcgga tgggggctgc gaagggttcc
       61 tgtgccgtgt tctcgacgct gttgaagacg atgaagacgt tgctgcgcgg gtagggggtg
      121 atgttgtccc cggagccgtg catggcgttg cagtcgaacc aggtggccga tccggctttg
      181 cccgtgaaga gcttgatgcc gtggcggtcg gccatcttcg tcagcgcctc gtcggagggt
      241 gtgccggcgt cctgcatctg cagggatttc ttgtagttgt ccttcggtgt ctcgccggag
      301 cagccgagga acgacttgtg ggagccgggc atgatcatga gcccgccgtt ggtgtcgtgg
      361 ttctcggtca gcgcgatgga gacggacacg gtccgcatgt tcggcaggcc gtcctcggcg
      421 tgccaggtct cgaagtcgga gtgccagtag aacccggagg cgccgaagcc gggcttgacg
      481 ttgatgcggg actggtggac gtagacgtcg gagccgagga tctggcgggc ccggcccacg
      541 acgcgttcgt cggcgacgag gcgggcgaag gcctcgctga gccggtggac ctcgaagacg
      601 gaccgcacgc tctgcgacgt cggctcgatg atcgagcgtt cgtcggcccg gacccgcgga
      661 tcggcgatca gccgttccag ctccgcgtgg tagccggcca cctcctgcgg ggagatgagc
      721 tggtcgacgg tgaggaagcc gtcgtgctcg aagccctgga ggtccttcgc ggagagcggc
      781 ccgggtgcgc cgggcgcgga ccagatgacc gggtcctggc ggggagtggt catctcggcg
      841 gcgccgcgcg aggggtacag gtcggctcgt acatcggtgg tcatggttca ggcctcctct
      901 gtcagcagtg ggtagacacc gttctcgtcg tggtcctccc gtccggtgac gggggggttg
      961 aagacgcaca cgcagcggaa gtcggtcttg ggccgcaggg tgtggtgttc gtggccgttc
     1021 agcaggtaca tcgtgccggg ggagatccag tgtgtctcgc cggtctcgtc gttggtgagc
     1081 tcggcctctc cctcggtgca cagcacggct tcgatgtggt tcgcgtacca catggacgtc
     1141 tccgtgccgg cgtagagcac ggtctcgtgg agcgagaagc cgaccttctc cttggcgagc
     1201 acgatgcgct tgctctccca ggttccggag gcggccttca catgccggtc ggtgttctcg
     1261 atgtcactga acgaacggac gatcacggtg ggatggtgcc tttctcttct gtgcttctca
     1321 ggtgtgcctg ccccggggcg tgccccggtc aggcggtctc gcggacggag cgggccagcg
     1381 tgcgcaggcc ctcgtccagc tcttcggggg tgatggtcag cggcgggagc agcttcacga
     1441 cctcgctctg ggggccggac gtctccagca gcagcccgag ctcgaaggcg cgcgcacaga
     1501 cggcggacgc gcgcgccggg tcggtgaact ccatgcccca gaccaggccg cggccacgga
     1561 actgcgcggt cggctcctcg gcgcagatcg ccaggagcgt ctgctccacc tgttcgccgc
     1621 gggccagggt ctgcttctcc atctggccgt ccgcccagta ggcgtcgagt gcggcggcgg
     1681 cggtgacgaa ggcggggttg ttgccgcgga aggtcccgtt gtgctcgccg ggctcccaga
     1741 tgtcgagctc gcccttgaag aggcacagtg acatcggcag gccgtagccg ctgatggact
     1801 tcgacagcgt gacgatgtcc gggacgatgc cggcctcctc gaaggagaag aagccgccgg
     1861 tacggccgca gcccatctgg atgtcgtcga cgatcaggag catgtcctgg cggtggcaca
     1921 ggtcctggag ggcgcggagc cattcggcgc gtgcgacgtt gatgccgccc tcgccctgca
     1981 cggtctccac gatcacggcc gcgggcttgt tcagcccgga gccctggtcc tccaggagcc
     2041 gctcgaacca caggaagtcg gggaccttgc cctcgaagta gttgtcgaac ggcatcgggg
     2101 tgccgtgcac cagcgggatg ccggcaccgg cccgcttgaa ggcgttgccg gtcacggcga
     2161 gcgagccgag cgacatgccg tggaaggcgt tggtgaagga gacgacggac tcgcggccct
     2221 tgaccttgcg ggcgagtttc agcgccgact cgacggcgtt ggtgccggtc gggccgggga
     2281 acatcacctt gtacggaagg tcgcgggggc gcaggatcac gttctcgaac gtctcgagga
     2341 acgcgcgttt ggcggtcgtg gccatgtcca ggccgtgcgt gatgccgtcg cgctcgatgt
     2401 agtcgatcag cgcgcgcttc agcaccgggt tgttgtggcc gtagttgagc gatccggcac
     2461 cggcgaagaa gtcgaggtac gagtgtccgt cctcgtcggt cagccgggcg ccctgcgcac
     2521 ggtcgaagac cgcgggccag ctgcggcagt agctccggac ttccgactca aggctctcga
     2581 agacactcag ggcgggcggg gtgatggtca cagcggatct cctgctcgag ggggtgtgac
     2641 ggacgggcgg cgcgtgaggc atgccccggg cgggggcgga gttcgggcct acgcgtggaa
     2701 cggaccgatg cggtacagca cttccggcag gtgcgtcccc tcggggaaca gctcgccgtc
     2761 gaagagcacc tcgcgctcca gcggtacgtc gtggcgctgt gcgaaggagg tgaacagccg
     2821 gtcggacgcg gtgttgtccg gggtgacggt cgtctcgacc gatgccaggc cctggtcgga
     2881 tgcgacacgc gtggtcagcg cgtccagcag ggtggcggcc aggcccttcc cgcggtgggc
     2941 gctgtcgacg gccacctgcc agacgacgag cgtcccgggc cggtccggac ggaggtaccc
     3001 cgtcacgaag ccgatggggc cgccggtctc gtcgcgggcg acgacggagg tcgccgcgaa
     3061 gtcacggcac cacagcaggt agctgtacga ggagttgagg tccaggacct cggaatcgcg
     3121 ggctatgcgc cagatcgcgg ctccgtcctc cactcgcggg gtgtcgatac tgaggaattc
     3181 gcttcgggca cgtgcaaaat ctgctggtgc ggcggtcatg cgaattgaat ttacccagca
     3241 aaaatctaaa ttgcatcgac gcaaggggtt gggtgagagg ggatgccgtg ttatcacgcg
     3301 ggcgcggacc gacttgtaag cactaccgat ttcaggcctt ttgccccgaa tttatcggtc
     3361 ataacggtca ctttgtgggg tcggtcacat gtgtgtaact ctcgtgacac ctgttcggat
     3421 tgagtgccgc gaccgcatgg gagctgaggt gtttaggaat gaggaaagcg ggcagaagaa
     3481 tgcggggagc tgcttcggat aaagcagctc cccgcattgt tgaattatcg ggtggattgc
     3541 ggtccgttca atcagtcgtt cggccaggtt tccgagacgg cattgcgggc accctcgaag
     3601 tcggcttccc gtccggtctc ggccagcgcg gcccccagcg cggccagcga ggcgtgcacg
     3661 gcggcccgcg tggcgtccac gccgtagtgg ttcacccgga tcatctccgt ggacagcgcg
     3721 ccgccgcccg cgatcagcgg cagcgagggg tcgcccgcga gggccttcgc gaccagcgcc
     3781 gaggcgtccg tccccgcggg cacccgcagc gtcgtggcga ccggcgccgc gtcacgggcg
     3841 tcgtggacgt acggctccag accggcaccg agggccaccg ctcccgcccg ggtggcagcg
     3901 gccgccgcgg cgtgccgggc cgtcagggtg tccagcccct ccgcctcgat ccgctccacg
     3961 caggcctcca gcgccagcat ctccagctgc gccggggcgt gcggcagggc cttgcggccc
     4021 ccgtcgatcc agcgggcctt ccagtcgagg agggagaggt aggagtgccg gggtgcctgc
     4081 gggttgccgg ccatccgctc ccatgcccgc tcgctcaccg acaccgcgga gaccccggcg
     4141 gggccgccca tcgccttctg tgcgccgatg acgcacaggt ccacgcccca gacgtccggc
     4201 agcagcggct ccgcgccgac cgacgcgacg gcgtccagca tgaagagcgc gccgtgcgcc
     4261 cggaccacct cgccgatctc cgcgaccggg ttggtgttgc cggtcgccgc ctcggcgtgc
     4321 accagcgaca cgaagtcgat ctccgggtgc tcggccagcg cctgcgccac ctggtcggcg
     4381 gtgaccgccg agtggaacgg cacggcgagg tcgacgacct cggccccgca gtcccgcagc
     4441 cagttgccga aggtctggcc gtacgggccc gtgacgacgt tcagcgcggt ggatccgggc
     4501 cgggcgccgc cccggatgca gccttcgagg ggcagcagcg cctcgccctg catgatgacg
     4561 acgtcctgcc cggtggcgag gaggccggcc acccgccgct cgatcgccgc gaaatgcgcg
     4621 gcggtgagcg gggcgaggtc gaggaagggg tgcgtcacgg tggtgctctc ttcgggtcgg
     4681 tcggtccgcg gtcagcgctt gcgtccgagg gtacccagca cctcccggag cccccggcgg
     4741 gccgcctcgt acagtgctgt ccatgagtga tcacgaggtg gcgcgggtgc tgcgggtcga
     4801 ggggcgggtg ctcgccggcc ccgacgacgt gagggacggc ctctgggccg tcggcggacg
     4861 catcacctac gagcggccgc cgggcgcgga cgacgccgtg acgctcaccg gctggtcgct
     4921 gccgggactc gtcgacgcgc actgccacgt cggcctcgac cgccatgggc ccgtcgacgc
     4981 ggcgaccagc gagaagcagg ccctcaccga ccgggcggcc ggaacgctgc tcatccggga
     5041 cgccggatcg ccctccgaca cccggtggat cgacgaccgc gaggacctgc cgaagatcat
     5101 cagagcgggc cggcacatcg ccaggacccg ccggtacatc aggaactacg cccatgagat
     5161 cgaacccggc gacctggtgg cgtacgtggc ccgggaggcg gagcgcggcg acgggtgggt
     5221 gaagctggtg ggcgactgga tcgaccggga cgccggtgac ctgacggcct gctggccccg
     5281 cggagaggtc gaggcggcca tcgccgaggc gcaccggctg ggtgcccggg tcaccgcgca
     5341 ctgcttcgcc gaggccgcgc tgcgcgacct ggtggaggcg gggatcgact gcatcgagca
     5401 cgccaccggg ctcaccgagg acaccatccc gctcttcgcc gagcgcggcg tcgcgatcgt
     5461 cccgaccctg gtcaacatcg ccacgttccc ggacctcgcg gcgggcggcg aggccaagtt
     5521 cccccgctgg tccgcccaca tgaggcagct gtacgagcgc cgctacgaca ccgtgcgcgc
     5581 ggcgtacgac gcgggcatcc cggtcttcgt cgggacggac gccggcggct cactggccca
     5641 cggcctggtg gcggccgagg tcgccgagct ggtgaaggcg ggcatcccgc ccctggaggc
     5701 gctctccgcg acggcctggg gcgcgcggcg ctggctgggc aggccggtgc tggaggaggg
     5761 cgccccggcg gacctggtgg tgtacgacga ggacccgcga gcggacgtac gggtgctggc
     5821 ggccccgcgg tgggtggtgc tcgacgggcg ggtggtcggc tgaccgcccg cgggcgtcgg
     5881 gttgaccggc ggtgaccccg gcgaccacgg ctcgttgaac cactttcgcc cctgttccgg
     5941 tgcccacgac gacggcgccg cccccgtggg gcgaaacggg tgactccggg gaactcctgt
     6001 gccggaagat tcgaattcgt acgcgaaaac ccccctttgg agtgaactga cggcaggtgt
     6061 ctgtcagttc accctctgtg cgtaaagatt cacggagtcg aggccaccgg tgcccgcgat
     6121 gtcccccatt ggacggcgcc ggcggctcca tgtctcttgt gggggttcca ccatcttgaa
     6181 cagcaacacc ttccgtctgg ccgccctcgc ggtcgccgcc gtacccgtcg ccctgctggc
     6241 cgccgtcccc gcccaggccg cgaccgcgac gatcccggcc accccg
//
