GCF_001426585.1 - 20 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_LMEI01000001.1 (Streptomyces sp. Root431)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	T1PKS,PKS-like,T2PKS,RiPP-like	1	96,562	Low	trioxacarcin A	PKS
Region 1.2	terpene	237,433	259,067
Region 1.3	NI-siderophore	625,812	655,602	High	desferrioxamin B/desferrioxamine E	other:other

NZ_LMEI01000004.1 (Streptomyces sp. Root431)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 4.1	T1PKS	407	24,671	High	desosamine	saccharide

NZ_LMEI01000005.1 (Streptomyces sp. Root431)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 5.1	T1PKS	1	8,159	Low	lenoremycin	PKS

NZ_LMEI01000006.1 (Streptomyces sp. Root431)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	indole	230,332	253,550	Low	rebeccamycin	other:other
Region 6.2	lanthipeptide-class-ii,CDPS	358,680	407,890	High	SBI-06990 A1/SBI-06989 A2	ribosomal:RiPP:Lanthipeptide
Region 6.3	NRP-metallophore,NRPS	441,166	498,325	Medium	peucechelin	NRPS:Type I
Region 6.4	RiPP-like	498,942	509,757

NZ_LMEI01000007.1 (Streptomyces sp. Root431)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 7.1	T1PKS	1	38,404	Low	notonesomycin A	other:other
Region 7.2	melanin	66,538	76,951
Region 7.3	terpene-precursor	342,483	363,547
Region 7.4	other	536,532	577,638

NZ_LMEI01000009.1 (Streptomyces sp. Root431)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	terpene-precursor	96,212	117,267
Region 9.2	terpene	143,328	169,931	High	hopene	terpene
Region 9.3	butyrolactone	518,893	529,879
Region 9.4	RiPP-like	642,095	653,534
Region 9.5	NI-siderophore	821,971	854,469	Low	kinamycin	PKS

NZ_LMEI01000012.1 (Streptomyces sp. Root431)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	NRPS-like	496,555	540,253	Low	lankamycin	PKS

NZ_LMEI01000013.1 (Streptomyces sp. Root431)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 13.1	NRPS-like,NRPS	341,342	390,441	High	bicornutin A1/bicornutin A2	NRPS:Type I

No secondary metabolite regions were found in these records:: NZ_LMEI01000002.1 (Streptomyces sp. Root431); NZ_LMEI01000003.1 (Streptomyces sp. Root431); NZ_LMEI01000008.1 (Streptomyces sp. Root431); NZ_LMEI01000010.1 (Streptomyces sp. Root431); NZ_LMEI01000011.1 (Streptomyces sp. Root431)

Compact view

NZ_LMEI01000001.1 - Region 1 - PKS-like,RiPP-like,T1PKS,T2PKS

Download region GenBank file

Download region SVG

Location: 1 - 96,562 nt. (total: 96,562 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)
T2PKS: (t2ks and t2clf)
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

ASC82_RS00080: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

ASC82_RS00115: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

ASC82_RS00200: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

ASC82_RS00210: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ASC82_RS00325: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

ASC82_RS00335: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ASC82_RS00340: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Transferases: PPTase acetyltransferase acylase glycosyltransferase

ASC82_RS00075: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ASC82_RS00110: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

ASC82_RS00155: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

ASC82_RS00195: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

ASC82_RS00215: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ASC82_RS00380: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Hydrolases: esterase phosphatase

ASC82_RS00040: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

ASC82_RS00060: thioesterase

smcogs: SMCOG1004: thioesterase

ASC82_RS00095: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

Lyases: cyclase

ASC82_RS00205: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Isomerases: mutase

ASC82_RS00300: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase synthase

ASC82_RS00015: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

ASC82_RS00065: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ASC82_RS00070: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ASC82_RS00100: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

ASC82_RS00170: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ASC82_RS00220: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ASC82_RS00225: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ASC82_RS00230: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

ASC82_RS31320: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Translocases: transport

ASC82_RS00240: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	1.29	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	1.24	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	1.17	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	1.03	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000231.5	0.99	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001160.5	0.97	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.95	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001275.3	0.88	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001257.3	0.85	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0000217.5	0.84	PKS	daunorubicin	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000231.5	0.73	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001276.3	0.68	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001160.5	0.67	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001535.3	0.66	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001164.3	0.66	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000234.5	0.66	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000111.5	0.64	PKS	neocarzilin A, neocarzilin B	Streptomyces carzinostaticus

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.77 out of 36.53

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

Additional weak hits (14):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.99 out of 29.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.19 out of 31.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.19 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.90 out of 30.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.76 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.12 out of 21.84

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.39 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.90 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.34 out of 28.19

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.24 out of 32.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.86 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.57 out of 36.53

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.97 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.57 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

RREFinder

Type II PKS

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 96562:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal) + (ccmal)
Non-colinear ordering:: ASC82_RS00075 + ASC82_RS00070

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 50586:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal) + (ccmal)
Non-colinear ordering:: ASC82_RS00075 + ASC82_RS00070

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 0 - 31883:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal) + (ccmal)
Non-colinear ordering:: ASC82_RS00075 + ASC82_RS00070

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 24079 - 96562:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal) + (ccmal)
Non-colinear ordering:: ASC82_RS00075 + ASC82_RS00070

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ASC82_RS00070: mal

PKS_AT (1725..2016): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.3
inactive: 128.1
Methoxymalonyl-CoA: 78.3
Methylmalonyl-CoA: 73.5
Isobutyryl-CoA: 53.2
Propionyl-CoA: 46.5
Ethylmalonyl-CoA: 41.8
Benzoyl-CoA: 38.4
trans-1,2-CPDA: 35.3
2-Methylbutyryl-CoA: 32.3
fatty_acid: 30.5
CHC-CoA: 25.7
Acetyl-CoA: 23.1
3-Methylbutyryl-CoA: 18.0

ASC82_RS00075: mmal

PKS_AT (542..838): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 83.3%; Malonyl-CoA: 79.2%; Methoxymalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 144.0
Methoxymalonyl-CoA: 112.6
Ethylmalonyl-CoA: 106.3
Malonyl-CoA: 98.3
Propionyl-CoA: 73.1
2-Methylbutyryl-CoA: 67.7
Isobutyryl-CoA: 67.1
Benzoyl-CoA: 59.6
trans-1,2-CPDA: 59.4
CHC-CoA: 57.0
Acetyl-CoA: 49.4
3-Methylbutyryl-CoA: 33.6
inactive: 25.6
fatty_acid: 23.4

ASC82_RS00170: X

AMP-binding (25..412): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GQILHPMHLN-AYAGTGGLVQGYGLTETAGLAHS
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ASC82_RS00215: mal

PKS_AT (18..306): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 53.6
Methoxymalonyl-CoA: 36.5
Methylmalonyl-CoA: 32.1
fatty_acid: 21.3
inactive: 14.7
Propionyl-CoA: 12.1
Isobutyryl-CoA: 10.3
Benzoyl-CoA: 8.4
3-Methylbutyryl-CoA: 8.1
CHC-CoA: 6.0
Ethylmalonyl-CoA: 5.6
trans-1,2-CPDA: 4.8
Acetyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

RRE predictions

: RRE type: Goadsporin_CD_RRE; Location: 2 - 99; Score: 59.7; RRE type: NHLP_CD_RRE; Location: 1 - 89; Score: 52.5; RRE type: Cyanobactin_D_RRE; Location: 2 - 76; Score: 36.3

Type II PKS gene cluster prediction details

Cluster section:: 24079 to 96562
Starter unit(s):: propionyl-CoA (Score: 668.0; E-value: 5.81e-139)
hexadienyl-CoA (Score: 108.0; E-value: 3.74e-28)
aziridinyl-CoA (Score: 21.2; E-value: 5.9)
Product class(es):: anthracycline
aureolic acid
tetracycline

ASC82_RS00075: AT (Score: 163.8; E-value: 1.2e-50); AT ADG86309.1_A-74528_hexadienyl-CoA (Score: 108.0; E-value: 3.74e-28); AT ADB02855.1_Azicemicin_aziridinyl-CoA (Score: 21.2; E-value: 5.9)
ASC82_RS00080: OXY (Score: 113.2; E-value: 3.6e-35)
ASC82_RS00105: ACP (Score: 92.6; E-value: 2.7e-29)
ASC82_RS00155: GT (Score: 102.2; E-value: 9.6e-32)
ASC82_RS00180: CYC C5-C14/C3-C16 (Score: 423.2; E-value: 8.6e-130)
ASC82_RS00190: ACP (Score: 112.3; E-value: 1.9e-35)
ASC82_RS00205: CYC C7-C12 (Score: 431.5; E-value: 3.4e-132)
ASC82_RS00210: KR C9 (Score: 454.0; E-value: 3.3e-139)
ASC82_RS00215: AT (Score: 373.2; E-value: 2.5e-114); AT AAF70110.1_Aclacinomycin_propionyl-CoA (Score: 278.0; E-value: 6.27e-95)
ASC82_RS00220: CLF (Score: 674.4; E-value: 1.2e-205)
ASC82_RS00225: KS (Score: 771.3; E-value: 4.9e-235)
ASC82_RS00230: KSIII (Score: 448.6; E-value: 3.2e-137); KSIII ABC00733.1_Cosmomycin_propionyl-CoA (Score: 390.0; E-value: 5.81e-139)
ASC82_RS00335: KR (Score: 119.9; E-value: 3.3e-37)

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
DmdR1	Iron(II)-dependent repressor
VqsM	Regulator of quorum-sensing signalling systems

NZ_LMEI01000001.1 - Region 2 - terpene

Download region GenBank file

Download region SVG

Location: 237,433 - 259,067 nt. (total: 21,635 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

ASC82_RS01130: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Hydrolases: protease

ASC82_RS01105: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Ligases: synthase

ASC82_RS01150: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001268.4	0.52	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000597.4	0.43	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0001075.5	0.43	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0000595.3	0.43	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000272.3	0.43	PKS	spore pigment	Streptomyces collinus
BGC0001659.3	0.42	terpene	mangicol A	Fusarium equiseti
BGC0001264.3	0.41	PKS	betaenone A, betaenone B, betaenone C	Phoma betae
BGC0000231.5	0.41	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000677.5	0.41	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001314.3	0.40	terpene	tirucalla	Arabidopsis thaliana

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001268.4	0.52	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000597.4	0.43	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0001075.5	0.43	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0000595.3	0.43	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000272.3	0.43	PKS	spore pigment	Streptomyces collinus
BGC0001659.3	0.42	terpene	mangicol A	Fusarium equiseti
BGC0001264.3	0.41	PKS	betaenone A, betaenone B, betaenone C	Phoma betae
BGC0000231.5	0.41	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000677.5	0.41	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001314.3	0.40	terpene	tirucalla	Arabidopsis thaliana

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene	drimane-type		C15	C2-C7 + C6-C11	PP

Genes with terpene domains:

ASC82_RS01140: T2TS

Domain 1 (32 - 538): Type 2 terpene synthase

Subtype: Sesquiterpene synthase, group b

Reactions:: FPP → sesquiterpene_C2-C7/C6-C11_PP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.37 out of 20.47

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

Additional weak hits (1):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
VqsM	Regulator of quorum-sensing signalling systems

NZ_LMEI01000001.1 - Region 3 - NI-siderophore

Download region GenBank file

Download region SVG

Location: 625,812 - 655,602 nt. (total: 29,791 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

ASC82_RS02765: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

ASC82_RS02785: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

Transferases: carboxylase transferase

ASC82_RS02750: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

ASC82_RS02755: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Hydrolases: hydrolase

ASC82_RS02810: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Lyases: decarboxylase

ASC82_RS02780: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: synthase

ASC82_RS02760: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000941.3	0.83	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001478.5	0.83	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002305.2	0.82	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000940.5	0.76	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.76	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0001572.3	0.70	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002464.3	0.61	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0002690.2	0.60	other (other)	FW0622	Verrucosispora sp. FIM060022
BGC0002284.3	0.58	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002620.2	0.55	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000941.3	0.83	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001478.5	0.83	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002305.2	0.82	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000940.5	0.76	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.76	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0001572.3	0.70	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002464.3	0.61	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0002690.2	0.60	other (other)	FW0622	Verrucosispora sp. FIM060022
BGC0002284.3	0.58	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002620.2	0.55	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 34.36 out of 36.53

Confidence: strong

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 30.77 out of 36.53

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 22.08 out of 27.04

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.39 out of 26.85

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.60 out of 29.72

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 30.11 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

Additional weak hits (3):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.68 out of 32.74

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.56 out of 39.62

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.48 out of 27.78

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CelR	Cellobiose uptake repressor
DasR	N-acetylglucosamine dependent repressor
DmdR1	Iron(II)-dependent repressor
NtrC	Nitrogen regulatory protein C
RutR	Regulator of the pyrimidine and purine metabolism

NZ_LMEI01000004.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 407 - 24,671 nt. (total: 24,265 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

ASC82_RS04350: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ASC82_RS04395: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase aminotransferase glycosyltransferase methyltransferase transferase

ASC82_RS04355: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

ASC82_RS04365: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

ASC82_RS04375: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

ASC82_RS04385: methyltransferase

smcogs: SMCOG1248: methyltransferase

ASC82_RS04390: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

ASC82_RS04410: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

ASC82_RS04400: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

ASC82_RS04370: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000898.5	0.90	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000899.4	0.84	saccharide	desosamine	Streptomyces narbonensis
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0002154.2	0.64	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000898.5	0.90	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000899.4	0.84	saccharide	desosamine	Streptomyces narbonensis
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0002154.2	0.64	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.67 out of 19.26

Confidence: medium

Additional weak hits (3):

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.36 out of 27.55

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.60 out of 35.63

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.45 out of 30.98

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 406 - 24671:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal)
Non-colinear ordering:: ASC82_RS04410 + ASC82_RS29840

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ASC82_RS04410: mmal

PKS_AT (560..804): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.1
Ethylmalonyl-CoA: 153.3
Methoxymalonyl-CoA: 140.4
2-Methylbutyryl-CoA: 111.1
Propionyl-CoA: 84.3
trans-1,2-CPDA: 83.9
CHC-CoA: 74.1
Malonyl-CoA: 65.6
Isobutyryl-CoA: 38.3
fatty_acid: 29.0
Benzoyl-CoA: 26.9
Acetyl-CoA: 14.7
inactive: 9.2
3-Methylbutyryl-CoA: 7.6

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_LMEI01000005.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 8,159 nt. (total: 8,159 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

ASC82_RS04435: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Ligases: synthase

ASC82_RS30915: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.63	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.60	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001268.4	0.58	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000041.3	0.58	PKS	oronofacic acid	Pseudomonas syringae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001284.4	0.63	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.60	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001268.4	0.58	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000041.3	0.58	PKS	oronofacic acid	Pseudomonas syringae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.82 out of 26.33

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 8159:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ASC82_RS30910: mmal

PKS_AT (0..132): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 58.3%; Methylmalonyl-CoA: 58.3%; Methoxymalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 86.8
Ethylmalonyl-CoA: 83.4
Methoxymalonyl-CoA: 75.1
2-Methylbutyryl-CoA: 64.1
trans-1,2-CPDA: 52.4
CHC-CoA: 51.6
Propionyl-CoA: 44.1
Malonyl-CoA: 35.5
Benzoyl-CoA: 15.8
Isobutyryl-CoA: 12.4
fatty_acid: 8.9
inactive: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ASC82_RS04435: mmal

PKS_AT (0..174): mmal

ATSignature: (unknown)

No matches above 50%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 91.6
Ethylmalonyl-CoA: 63.2
Methoxymalonyl-CoA: 57.5
Propionyl-CoA: 33.5
2-Methylbutyryl-CoA: 28.9
Isobutyryl-CoA: 19.0
Malonyl-CoA: 18.8
trans-1,2-CPDA: 17.8
Acetyl-CoA: 12.8
fatty_acid: 11.3
inactive: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_LMEI01000006.1 - Region 1 - indole

Download region GenBank file

Download region SVG

Location: 230,332 - 253,550 nt. (total: 23,219 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

ASC82_RS05565: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ASC82_RS05570: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

ASC82_RS05585: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: aminotransferase

ASC82_RS05590: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: hydrolase

ASC82_RS05545: isochorismatase

smcogs: SMCOG1027: isochorismatase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001224.5	0.62	other (other)	reductasporine	uncultured bacterium
BGC0001223.5	0.61	other (other)	hydroxysporine	uncultured bacterium
BGC0001336.5	0.59	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0000817.4	0.54	other (other)	methylarcyriarubin	uncultured bacterium
BGC0000829.5	0.48	other (other)	violacein	Chromobacterium violaceum
BGC0000821.5	0.47	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0000813.3	0.46	other (other)	K-252a	Nonomuraea longicatena
BGC0000830.5	0.46	other (aminocoumarin)	violacein	uncultured bacterium
BGC0000902.4	0.45	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000831.3	0.44	other (other)	violacein	Duganella sp. B2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001224.5	0.62	other (other)	reductasporine	uncultured bacterium
BGC0001223.5	0.61	other (other)	hydroxysporine	uncultured bacterium
BGC0001336.5	0.59	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0000817.4	0.54	other (other)	methylarcyriarubin	uncultured bacterium
BGC0000829.5	0.48	other (other)	violacein	Chromobacterium violaceum
BGC0000821.5	0.47	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0000813.3	0.46	other (other)	K-252a	Nonomuraea longicatena
BGC0000830.5	0.46	other (aminocoumarin)	violacein	uncultured bacterium
BGC0000902.4	0.45	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000831.3	0.44	other (other)	violacein	Duganella sp. B2

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 25.93 out of 32.38

Confidence: medium

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 26.83 out of 32.93

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

Additional weak hits (12):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.05 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.63 out of 30.25

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 23.36 out of 33.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.38 out of 31.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.42 out of 29.38

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.65 out of 31.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 16.46 out of 36.54

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.05 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.42 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.04 out of 28.19

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 23.35 out of 32.38

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.35 out of 27.18

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CsoR	Copper-responsive repressor
RicR	Copper-responsive regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_LMEI01000006.1 - Region 2 - CDPS,lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 358,680 - 407,890 nt. (total: 49,211 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)
CDPS: CDPS

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

ASC82_RS06175: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

ASC82_RS06245: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: methyltransferase

ASC82_RS06225: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: hydrolase

ASC82_RS06255: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: cyclase decarboxylase dehydratase

ASC82_RS06120: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

ASC82_RS06135: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

ASC82_RS06145: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

ASC82_RS06170: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: synthase

ASC82_RS06105: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Translocases: transporter

ASC82_RS06125: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.37	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000503.5	0.35	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000520.5	0.35	ribosomal	lacticin 3147 A1, lacticin 3147 A2	Lactococcus lactis
BGC0002110.3	0.35	ribosomal	birimositide	Streptomyces rimosus subsp. rimosus WC-3908
BGC0000517.4	0.30	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0001229.4	0.30	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0000548.4	0.29	ribosomal	salivaricin A	Streptococcus salivarius
BGC0002663.2	0.29	ribosomal	amyloliquecidin GF610	Bacillus amyloliquefaciens UMAF6639
BGC0002346.3	0.28	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000501.3	0.28	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002110.3	0.68	ribosomal	birimositide	Streptomyces rimosus subsp. rimosus WC-3908
BGC0001229.4	0.63	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0001986.3	0.59	other (other)	purincyclamide	Streptomyces chrestomyceticus
BGC0000520.5	0.58	ribosomal	lacticin 3147 A1, lacticin 3147 A2	Lactococcus lactis
BGC0000528.5	0.56	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000503.5	0.56	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002631.2	0.55	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0000623.3	0.53	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0001311.5	0.52	ribosomal	flavecins	Ruminococcus flavefaciens FD-1
BGC0000517.4	0.52	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ASC82_RS06145

ASC82_RS06140

Leader:

MNNFDGDISLIEEIAEQDFDGVAYGA

Core:

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

Core with dehydrations:

CysDhaDhbNDhbFDhaLDhaDFLGNQGGWCysDhbLDhbKECysQPNCysN

antiSMASH-DB 4.0 matches for ASC82_RS06140

100.0%

NZ_AKXV01000031.1and 4 others:

NZ_AKXV01000031.1 Lanthipeptides: Y19_RS0109895_lanthipeptideNZ_JABBGE010000004.1 Lanthipeptides: HHL19_RS13545_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS06140_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS06140_lanthipeptide

Lanthipeptides: Y19_RS0109895_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

93.3%

NZ_CP107567.1and 3 others:

NZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptide

Lanthipeptides: OGH68_RS19760_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD+LGNQGGWCTLTKECQPNCN

1

CTTNTFSLSDWLGNQGGWCTLTKECQPNCN

30

90.0%

NZ_BNBV01000031.1and 1 other:

NZ_BNBV01000031.1 Lanthipeptides: IE286_RS22995_lanthipeptide

Lanthipeptides: IE286_RS22995_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLTKECQPNCN

1

CTTNTFSLSDALGNSGGWCTLTKECQPNCN

30

with 102 more sequence matches

Only the first 10 are displayed here.

86.7%

NZ_BJTV01000004.1and 1 other:

NZ_BJTV01000004.1 Lanthipeptides: TNCT1_RS10200_lanthipeptide

Lanthipeptides: TNCT1_RS10200_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLTKECQ NCN

1

CTTNTFSLSDALGNHGGWCTLTKECQANCN

30

86.7%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: IEY56_RS28370_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS07545_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS07545_lanthipeptide

Lanthipeptides: IEY56_RS28370_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLT ECQPNCN

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

86.7%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: IE314_RS15865_lanthipeptide

Lanthipeptides: IE314_RS15865_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD +GN GGWCTLTKECQPNCN

1

CTTNTFSLSDAVGNHGGWCTLTKECQPNCN

30

86.7%

NZ_CP016795.1and 5 others:

NZ_CP016795.1 Lanthipeptides: BC342_RS18360_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18960_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18960_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01495_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01495_lanthipeptide

Lanthipeptides: BC342_RS18360_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLTKECQPNC+

1

CTTNTFSLSDALGNHGGWCTLTKECQPNCS

30

86.7%

NZ_CP043317.1and 1 other:

NZ_CP043317.1 Lanthipeptides: FYC74_RS17680_lanthipeptide

Lanthipeptides: FYC74_RS17680_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLTKECQPNC+

1

CTTNTFSLSDSLGNHGGWCTLTKECQPNCS

30

86.7%

NZ_QLLX01000002.1and 1 other:

NZ_QLLX01000002.1 Lanthipeptides: K378_RS05175_lanthipeptide

Lanthipeptides: K378_RS05175_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD L NQGGWCTLT ECQPNCN

1

CTTNTFSLSDALTNQGGWCTLTVECQPNCN

30

83.3%

NZ_JAASAG010000001.1and 1 other:

NZ_JAASAG010000001.1 Lanthipeptides: HCC61_RS30395_lanthipeptide

Lanthipeptides: HCC61_RS30395_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD L N GGWCTLT ECQPNCN

1

CTTNTFSLSDALTNTGGWCTLTVECQPNCN

30

80.0%

NZ_CP047419.1and 3 others:

NZ_CP047419.1 Lanthipeptides: GTU71_RS12805_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptide

Lanthipeptides: GTU71_RS12805_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNC

29

C+TNTF+LS+ LGNQGGWCTLT ECQPNC

1

CTTNTFTLSNVLGNQGGWCTLTVECQPNC

29

80.0%

NZ_JAJAGO010000028.1and 1 other:

NZ_JAJAGO010000028.1 Lanthipeptides: LHJ74_RS33590_lanthipeptide

Lanthipeptides: LHJ74_RS33590_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

CSTNTFSLSD GN G WCTLTKECQ CN

1

CSTNTFSLSDAAGNDGWWCTLTKECQRTCN

30

80.0%

NZ_LT985747.1and 1 other:

NZ_LT985747.1 Lanthipeptides: C8268_RS00165_lanthipeptide

Lanthipeptides: C8268_RS00165_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTF+LSD+LGN+GGWCTLTKECQ +CN

1

CTTNTFTLSDYLGNKGGWCTLTKECQRSCN

30

MIBiG 4.0 matches for ASC82_RS06140

86.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLS+ LGN GGWCTLTKECQPNCN

1

CTTNTFSLSNALGNNGGWCTLTKECQPNCN

30

46.7%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

6

FSLSDFLGNQGGWCTLTKECQPNCN

30

+++S LGN+G +CTLT EC P+CN

4

YNISCRLGNKGAYCTLTVECMPSCN

28

28.2%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

19

CTLTKECQPNCN

30

CTL+KECQPNCN

28

CTLSKECQPNCN

39

ASC82_RS30550

Leader:

MEKMQADVLGGYDEFELVEMGEADVYGG

Core:

TTWSCAIVTAVATLVSASACPTTKCTSQC

Core with dehydrations:

DhbDhbWDhaCysAIVDhbAVADhbLVDhaADhaACysPDhbDhbKCysDhbDhaQCys

antiSMASH-DB 4.0 matches for ASC82_RS30550

96.6%

NZ_JABBGE010000004.1and 1 other:

NZ_JABBGE010000004.1 Lanthipeptides: HHL19_RS13570_lanthipeptide

Lanthipeptides: HHL19_RS13570_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

TTWSCA+VTAVATLVSASACPTTKCTSQC

1

TTWSCAVVTAVATLVSASACPTTKCTSQC

29

69.0%

NZ_CP042598.1and 3 others:

NZ_CP042598.1 Lanthipeptides: FUT28_RS13815_lanthipeptideNZ_RKNR01000012.1 Lanthipeptides: EGW35_RS11625_lanthipeptideNZ_RKNR01000012.1 Lanthipeptides: EGW35_RS11625_lanthipeptide

Lanthipeptides: FUT28_RS13815_lanthipeptide

1

TTWSCAIVTAVATLVSAS--ACPTTKCTSQC

29

TTW+C AV+T+V AS CPTTKCTSQC

1

TTWACV---AVSTIVDASISLCPTTKCTSQC

28

65.5%

NZ_JADPDT010000005.1and 1 other:

NZ_JADPDT010000005.1 Lanthipeptides: I4V19_RS09875_lanthipeptide

Lanthipeptides: I4V19_RS09875_lanthipeptide

1

TTWSCAIVTAVATLVSAS--ACPTTKCTSQC

29

TTW+C AV+T+V AS CPTTKCTS+C

1

TTWACV---AVSTVVEASISLCPTTKCTSKC

28

with 97 more sequence matches

Only the first 10 are displayed here.

63.0%

NZ_AKXV01000031.1and 3 others:

NZ_AKXV01000031.1 Lanthipeptides: Y19_RS44045_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS30550_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS30550_lanthipeptide

Lanthipeptides: Y19_RS44045_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

TTWSCAIVTAVATLVSASACPTTKCTSQC

18

TTWSCAIVTAVATLVSASACPTTKCTSQC

46

48.7%

NZ_CP009288.1and 3 others:

NZ_CP009288.1 Lanthipeptides: PDUR_RS29295_lanthipeptideNZ_CP076607.1 Lanthipeptides: KP014_RS01575_lanthipeptideNZ_CP076607.1 Lanthipeptides: KP014_RS01575_lanthipeptide

Lanthipeptides: PDUR_RS29295_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

++W+C +TA T VSAS CPTTKCTSQC

14

SSWTC--ITAGVT-VSASLCPTTKCTSQC

39

48.5%

NZ_QORY01000016.1and 1 other:

NZ_QORY01000016.1 Lanthipeptides: DT250_RS18265_lanthipeptide

Lanthipeptides: DT250_RS18265_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

TTW CAI T + SA+ CPTT C+S+C

8

TTWGCAIFT----IASAAQCPTTACSSKC

32

47.4%

NC_006322.1and 7 others:

NC_006322.1 Lanthipeptides: BLI_RS20290_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07810_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07810_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08830_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08830_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11870_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11870_lanthipeptide

Lanthipeptides: BLI_RS20290_lanthipeptide

3

WSCAIVTAVATLVSASACPTTKCTSQC

29

W+C +TA T VSAS CPTTKCTS+C

15

WTC--ITAGVT-VSASLCPTTKCTSRC

38

42.9%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: IEY56_RS09415_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS29345_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS29345_lanthipeptide

Lanthipeptides: IEY56_RS09415_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

T W CA VT L +A+ CPTTKCTSQC

18

TAWVCATVT----LATAAICPTTKCTSQC

42

41.9%

NZ_CP082942.1and 1 other:

NZ_CP082942.1 Lanthipeptides: K8O96_RS09115_lanthipeptide

Lanthipeptides: K8O96_RS09115_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

T +CAI A V+A ACP+ CT C

2

TGLACAITGATVAAVTAVACPSGACTGYC

30

41.4%

NZ_BIFH01000022.1and 1 other:

NZ_BIFH01000022.1 Lanthipeptides: EMA17_RS52585_lanthipeptide

Lanthipeptides: EMA17_RS52585_lanthipeptide

6

AIVTAVATLVSASACPTTKCTSQC

29

A+ A TL++A+ CPTT C+S+C

5

ALCVATITLITATMCPTTACSSKC

28

41.4%

NZ_LT630004.1:

Lanthipeptides: BQ5796_RS18310_lanthipeptide

10

AVATLVSASACPTTKCTSQC

29

+ +S S CPTTKCTSQC

8

GITVTISVSMCPTTKCTSQC

27

40.6%

NZ_CP013862.1and 1 other:

NZ_CP013862.1 Lanthipeptides: AOX59_RS09365_lanthipeptide

Lanthipeptides: AOX59_RS09365_lanthipeptide

15

VSASACPTTKCTSQC

29

VSA CPTTKCTSQC

18

VSALFCPTTKCTSQC

32

40.5%

NZ_PNGD01000001.1and 1 other:

NZ_PNGD01000001.1 Lanthipeptides: CJ239_RS00165_lanthipeptide

Lanthipeptides: CJ239_RS00165_lanthipeptide

2

TWSCAIVTAVATLVSASACPTTKCTSQC

29

T S A VT TL +A ACPT+ CT C

9

TISFAGVTFTVTL-NAGACPTSGCTKSC

35

MIBiG 4.0 matches for ASC82_RS30550

43.2%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

6

AIVTAVATLVSASACPTTKCTSQC

29

AI VA VSA+ CPTTKCTS+C

14

AIQAGVAVGVSAAFCPTTKCTSRC

37

31.0%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

20

CPTTKCTSQC

29

CPTTKCTS+C

17

CPTTKCTSKC

26

20.7%

BGC0002130.3 (QKI29070.1):

Lanthipeptide: cacaoidin

5

CAIVTAVATLVSASA

19

C I +V+ +SA+A

5

CTIYASVSASISATA

19

Lanthipeptide(s) for ASC82_RS06170

ASC82_RS06140

Leader:

MNNFDGDISLIEEIAEQDFDGVAYGA

Core:

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

Core with dehydrations:

CysDhaDhbNDhbFDhaLDhaDFLGNQGGWCysDhbLDhbKECysQPNCysN

antiSMASH-DB 4.0 matches for ASC82_RS06140

100.0%

NZ_AKXV01000031.1and 4 others:

NZ_AKXV01000031.1 Lanthipeptides: Y19_RS0109895_lanthipeptideNZ_JABBGE010000004.1 Lanthipeptides: HHL19_RS13545_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS06140_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS06140_lanthipeptide

Lanthipeptides: Y19_RS0109895_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

93.3%

NZ_CP107567.1and 3 others:

NZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptide

Lanthipeptides: OGH68_RS19760_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD+LGNQGGWCTLTKECQPNCN

1

CTTNTFSLSDWLGNQGGWCTLTKECQPNCN

30

90.0%

NZ_BNBV01000031.1and 1 other:

NZ_BNBV01000031.1 Lanthipeptides: IE286_RS22995_lanthipeptide

Lanthipeptides: IE286_RS22995_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLTKECQPNCN

1

CTTNTFSLSDALGNSGGWCTLTKECQPNCN

30

with 102 more sequence matches

Only the first 10 are displayed here.

86.7%

NZ_BJTV01000004.1and 1 other:

NZ_BJTV01000004.1 Lanthipeptides: TNCT1_RS10200_lanthipeptide

Lanthipeptides: TNCT1_RS10200_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLTKECQ NCN

1

CTTNTFSLSDALGNHGGWCTLTKECQANCN

30

86.7%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: IEY56_RS28370_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS07545_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS07545_lanthipeptide

Lanthipeptides: IEY56_RS28370_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLT ECQPNCN

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

86.7%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: IE314_RS15865_lanthipeptide

Lanthipeptides: IE314_RS15865_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD +GN GGWCTLTKECQPNCN

1

CTTNTFSLSDAVGNHGGWCTLTKECQPNCN

30

86.7%

NZ_CP016795.1and 5 others:

NZ_CP016795.1 Lanthipeptides: BC342_RS18360_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18960_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18960_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01495_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01495_lanthipeptide

Lanthipeptides: BC342_RS18360_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLTKECQPNC+

1

CTTNTFSLSDALGNHGGWCTLTKECQPNCS

30

86.7%

NZ_CP043317.1and 1 other:

NZ_CP043317.1 Lanthipeptides: FYC74_RS17680_lanthipeptide

Lanthipeptides: FYC74_RS17680_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD LGN GGWCTLTKECQPNC+

1

CTTNTFSLSDSLGNHGGWCTLTKECQPNCS

30

86.7%

NZ_QLLX01000002.1and 1 other:

NZ_QLLX01000002.1 Lanthipeptides: K378_RS05175_lanthipeptide

Lanthipeptides: K378_RS05175_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD L NQGGWCTLT ECQPNCN

1

CTTNTFSLSDALTNQGGWCTLTVECQPNCN

30

83.3%

NZ_JAASAG010000001.1and 1 other:

NZ_JAASAG010000001.1 Lanthipeptides: HCC61_RS30395_lanthipeptide

Lanthipeptides: HCC61_RS30395_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLSD L N GGWCTLT ECQPNCN

1

CTTNTFSLSDALTNTGGWCTLTVECQPNCN

30

80.0%

NZ_CP047419.1and 3 others:

NZ_CP047419.1 Lanthipeptides: GTU71_RS12805_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptide

Lanthipeptides: GTU71_RS12805_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNC

29

C+TNTF+LS+ LGNQGGWCTLT ECQPNC

1

CTTNTFTLSNVLGNQGGWCTLTVECQPNC

29

80.0%

NZ_JAJAGO010000028.1and 1 other:

NZ_JAJAGO010000028.1 Lanthipeptides: LHJ74_RS33590_lanthipeptide

Lanthipeptides: LHJ74_RS33590_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

CSTNTFSLSD GN G WCTLTKECQ CN

1

CSTNTFSLSDAAGNDGWWCTLTKECQRTCN

30

80.0%

NZ_LT985747.1and 1 other:

NZ_LT985747.1 Lanthipeptides: C8268_RS00165_lanthipeptide

Lanthipeptides: C8268_RS00165_lanthipeptide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTF+LSD+LGN+GGWCTLTKECQ +CN

1

CTTNTFTLSDYLGNKGGWCTLTKECQRSCN

30

MIBiG 4.0 matches for ASC82_RS06140

86.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

C+TNTFSLS+ LGN GGWCTLTKECQPNCN

1

CTTNTFSLSNALGNNGGWCTLTKECQPNCN

30

46.7%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

6

FSLSDFLGNQGGWCTLTKECQPNCN

30

+++S LGN+G +CTLT EC P+CN

4

YNISCRLGNKGAYCTLTVECMPSCN

28

28.2%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

19

CTLTKECQPNCN

30

CTL+KECQPNCN

28

CTLSKECQPNCN

39

ASC82_RS30550

Leader:

MEKMQADVLGGYDEFELVEMGEADVYGG

Core:

TTWSCAIVTAVATLVSASACPTTKCTSQC

Core with dehydrations:

DhbDhbWDhaCysAIVDhbAVADhbLVDhaADhaACysPDhbDhbKCysDhbDhaQCys

antiSMASH-DB 4.0 matches for ASC82_RS30550

96.6%

NZ_JABBGE010000004.1and 1 other:

NZ_JABBGE010000004.1 Lanthipeptides: HHL19_RS13570_lanthipeptide

Lanthipeptides: HHL19_RS13570_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

TTWSCA+VTAVATLVSASACPTTKCTSQC

1

TTWSCAVVTAVATLVSASACPTTKCTSQC

29

69.0%

NZ_CP042598.1and 3 others:

NZ_CP042598.1 Lanthipeptides: FUT28_RS13815_lanthipeptideNZ_RKNR01000012.1 Lanthipeptides: EGW35_RS11625_lanthipeptideNZ_RKNR01000012.1 Lanthipeptides: EGW35_RS11625_lanthipeptide

Lanthipeptides: FUT28_RS13815_lanthipeptide

1

TTWSCAIVTAVATLVSAS--ACPTTKCTSQC

29

TTW+C AV+T+V AS CPTTKCTSQC

1

TTWACV---AVSTIVDASISLCPTTKCTSQC

28

65.5%

NZ_JADPDT010000005.1and 1 other:

NZ_JADPDT010000005.1 Lanthipeptides: I4V19_RS09875_lanthipeptide

Lanthipeptides: I4V19_RS09875_lanthipeptide

1

TTWSCAIVTAVATLVSAS--ACPTTKCTSQC

29

TTW+C AV+T+V AS CPTTKCTS+C

1

TTWACV---AVSTVVEASISLCPTTKCTSKC

28

with 97 more sequence matches

Only the first 10 are displayed here.

63.0%

NZ_AKXV01000031.1and 3 others:

NZ_AKXV01000031.1 Lanthipeptides: Y19_RS44045_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS30550_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS30550_lanthipeptide

Lanthipeptides: Y19_RS44045_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

TTWSCAIVTAVATLVSASACPTTKCTSQC

18

TTWSCAIVTAVATLVSASACPTTKCTSQC

46

48.7%

NZ_CP009288.1and 3 others:

NZ_CP009288.1 Lanthipeptides: PDUR_RS29295_lanthipeptideNZ_CP076607.1 Lanthipeptides: KP014_RS01575_lanthipeptideNZ_CP076607.1 Lanthipeptides: KP014_RS01575_lanthipeptide

Lanthipeptides: PDUR_RS29295_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

++W+C +TA T VSAS CPTTKCTSQC

14

SSWTC--ITAGVT-VSASLCPTTKCTSQC

39

48.5%

NZ_QORY01000016.1and 1 other:

NZ_QORY01000016.1 Lanthipeptides: DT250_RS18265_lanthipeptide

Lanthipeptides: DT250_RS18265_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

TTW CAI T + SA+ CPTT C+S+C

8

TTWGCAIFT----IASAAQCPTTACSSKC

32

47.4%

NC_006322.1and 7 others:

NC_006322.1 Lanthipeptides: BLI_RS20290_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07810_lanthipeptideNZ_CP033218.1 Lanthipeptides: D9Y32_RS07810_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08830_lanthipeptideNZ_LQYL01000021.1 Lanthipeptides: B4090_RS08830_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11870_lanthipeptideNZ_NIMP01000036.1 Lanthipeptides: CHCC14566_RS11870_lanthipeptide

Lanthipeptides: BLI_RS20290_lanthipeptide

3

WSCAIVTAVATLVSASACPTTKCTSQC

29

W+C +TA T VSAS CPTTKCTS+C

15

WTC--ITAGVT-VSASLCPTTKCTSRC

38

42.9%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: IEY56_RS09415_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS29345_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS29345_lanthipeptide

Lanthipeptides: IEY56_RS09415_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

T W CA VT L +A+ CPTTKCTSQC

18

TAWVCATVT----LATAAICPTTKCTSQC

42

41.9%

NZ_CP082942.1and 1 other:

NZ_CP082942.1 Lanthipeptides: K8O96_RS09115_lanthipeptide

Lanthipeptides: K8O96_RS09115_lanthipeptide

1

TTWSCAIVTAVATLVSASACPTTKCTSQC

29

T +CAI A V+A ACP+ CT C

2

TGLACAITGATVAAVTAVACPSGACTGYC

30

41.4%

NZ_BIFH01000022.1and 1 other:

NZ_BIFH01000022.1 Lanthipeptides: EMA17_RS52585_lanthipeptide

Lanthipeptides: EMA17_RS52585_lanthipeptide

6

AIVTAVATLVSASACPTTKCTSQC

29

A+ A TL++A+ CPTT C+S+C

5

ALCVATITLITATMCPTTACSSKC

28

41.4%

NZ_LT630004.1:

Lanthipeptides: BQ5796_RS18310_lanthipeptide

10

AVATLVSASACPTTKCTSQC

29

+ +S S CPTTKCTSQC

8

GITVTISVSMCPTTKCTSQC

27

40.6%

NZ_CP013862.1and 1 other:

NZ_CP013862.1 Lanthipeptides: AOX59_RS09365_lanthipeptide

Lanthipeptides: AOX59_RS09365_lanthipeptide

15

VSASACPTTKCTSQC

29

VSA CPTTKCTSQC

18

VSALFCPTTKCTSQC

32

40.5%

NZ_PNGD01000001.1and 1 other:

NZ_PNGD01000001.1 Lanthipeptides: CJ239_RS00165_lanthipeptide

Lanthipeptides: CJ239_RS00165_lanthipeptide

2

TWSCAIVTAVATLVSASACPTTKCTSQC

29

T S A VT TL +A ACPT+ CT C

9

TISFAGVTFTVTL-NAGACPTSGCTKSC

35

MIBiG 4.0 matches for ASC82_RS30550

43.2%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

6

AIVTAVATLVSASACPTTKCTSQC

29

AI VA VSA+ CPTTKCTS+C

14

AIQAGVAVGVSAAFCPTTKCTSRC

37

31.0%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

20

CPTTKCTSQC

29

CPTTKCTS+C

17

CPTTKCTSKC

26

20.7%

BGC0002130.3 (QKI29070.1):

Lanthipeptide: cacaoidin

5

CAIVTAVATLVSASA

19

C I +V+ +SA+A

5

CTIYASVSASISATA

19

ASC82_RS06200

Leader:

MMRTATGKAARGIAAAMLTALATTGAFTALVGSVRPASGDAVVVAGG

Core:

CDPLDACWVNGWQEKPGT

Core with dehydrations:

CysDPLDACysWVNGWQEKPGDhb

antiSMASH-DB 4.0 matches for ASC82_RS06200

100.0%

NZ_LMEI01000006.1:

Lanthipeptides: ASC82_RS06200_lanthipeptide

1

CDPLDACWVNGWQEKPGT

18

CDPLDACWVNGWQEKPGT

1

CDPLDACWVNGWQEKPGT

18

84.2%

NZ_AKXV01000031.1:

Lanthipeptides: Y19_RS0109830_lanthipeptide

2

DPLDACWVNGWQEKPGT

18

DPL+ACWVNGWQEKPGT

3

DPLNACWVNGWQEKPGT

19

31.8%

NZ_FOFV01000025.1:

Lanthipeptides: allorf_010544_010660_lanthipeptide

2

DPLDACWVNGWQEKPGT

18

P AC G++ PGT

5

KPRTACCGPGFRVFPGT

21

with 3 more sequence matches

17.5%

NZ_JABBGE010000004.1:

Lanthipeptides: HHL19_RS13610_lanthipeptide

9

VNGWQEKP

16

VNGWQE P

31

VNGWQETP

38

11.8%

NC_009621.1and 2 others:

NZ_CP088111.1 Lanthipeptides: allorf_0781198_0781362_lanthipeptideNZ_VITA01000039.1 Lanthipeptides: allorf_12218_12382_lanthipeptide

Lanthipeptides: allorf_1014954_1015118_lanthipeptide

7

CWVNGWQEKP

16

CW+ W+ +P

21

CWLQAWESRP

30

10.2%

NZ_CP007597.1:

Lanthipeptides: allorf_2301180_2301410_lanthipeptide

6

ACWVNGWQEKPG

17

ACW +E+PG

35

ACWCGLGKERPG

46

allorf_383679_383804

Leader:

MVHEQEALPWEGTSGKIRPACAAGIVGG

Core:

RCPKGKGFPRSPW

Core with dehydrations:

RCysPKGKGFPRDhaPW

antiSMASH-DB 4.0 matches for allorf_383679_383804

100.0%

NZ_LMEI01000006.1:

Lanthipeptides: allorf_383679_383804_lanthipeptide

1

RCPKGKGFPRSPW

13

RCPKGKGFPRSPW

1

RCPKGKGFPRSPW

13

23.1%

NZ_VEAF01000006.1:

Lanthipeptides: allorf_172444_172557_lanthipeptide

1

RCPKGKGFPRSPW

13

RC +G R+PW

11

RCSRGCNVLRTPW

23

13.0%

NZ_FWYC01000014.1:

Lanthipeptides: allorf_164645_164809_lanthipeptide

1

RCPKGKGFPRS

11

RCP +G PR

28

RCPAERGLPRQ

38

allorf_385739_385885

Leader:

MGLGRVSKVASSARRGGLAAS

Core:

GACDRKAEGRPDTGCIGVIPTTRRGGT

Core with dehydrations:

GACysDRKAEGRPDDhbGCysIGVIPDhbDhbRRGGDhb

antiSMASH-DB 4.0 matches for allorf_385739_385885

100.0%

NZ_LMEI01000006.1:

Lanthipeptides: allorf_385739_385885_lanthipeptide

1

GACDRKAEGRPDTGCIGVIPTTRRGGT

27

GACDRKAEGRPDTGCIGVIPTTRRGGT

1

GACDRKAEGRPDTGCIGVIPTTRRGGT

27

37.9%

NZ_JNLT01000008.1and 2 others:

NZ_JNLT01000008.1 Lanthipeptides: allorf_027788_028009_lanthipeptideNZ_JNLT01000008.1 Lanthipeptides: allorf_027788_028009_lanthipeptide

Lanthipeptides: allorf_027788_028009_lanthipeptide

9

GRPDTGCIGVIPTTRRG

25

GRP TGC G T RRG

13

GRPPTGCGGRPSTGRRG

29

29.6%

NZ_FOLM01000007.1:

Lanthipeptides: allorf_089218_089310_lanthipeptide

9

GRPDTGCIGVIPTTRRG

25

GRP GC G + R G

1

GRPARGCAGSLRAFRGG

17

with 3 more sequence matches

22.2%

NZ_JACHJG010000002.1:

Lanthipeptides: FHS38_RS07010_lanthipeptide

10

RPDTGCI

16

PDTGCI

18

NPDTGCI

24

17.0%

NZ_CP013140.1and 2 others:

NZ_CP067396.1 Lanthipeptides: allorf_0993107_0993301_lanthipeptideNZ_CP110813.1 Lanthipeptides: allorf_0962390_0962584_lanthipeptide

Lanthipeptides: allorf_4964629_4964823_lanthipeptide

1

GACDRKAEGRPDTG

14

G DRK GRP+T

5

GEADRKMSGRPETA

18

15.3%

NZ_BJTV01000019.1:

Lanthipeptides: allorf_110642_110857_lanthipeptide

6

KAEGRPDTGCIGVIPTTR

23

+AEGR GV P TR

3

QAEGRHHERRFGVRPPTR

20

MIBiG 4.0 matches for allorf_385739_385885

14.8%

BGC0000563.4 (AEA03263.1):

Lanthipeptide: venezuelin

12

DTGCIGV

18

+T CIG+

13

NTVCIGI

19

allorf_386141_386362

Leader:

MVLPLPAPRRRFLRGA

Core:

RDEIPDHIRSIAANPPPPQDSPLACSCLRTVHDPATAQGPGSWAKVSWSRRVLAASP

Core with dehydrations:

RDEIPDHIRDhaIAANPPPPQDDhaPLACysDhaCysLRDhbVHDPADhbAQGPGDhaWAKVDhaWDhaRRVLAADhaP

antiSMASH-DB 4.0 matches for allorf_386141_386362

100.0%

NZ_LMEI01000006.1:

Lanthipeptides: allorf_386141_386362_lanthipeptide

1

RDEIPDHIRSIAANPPPPQDSPLACSCLRTVHDPATAQGPGSWAKVSWSRRVLAASP

57

RDEIPDHIRSIAANPPPPQDSPLACSCLRTVHDPATAQGPGSWAKVSWSRRVLAASP

1

RDEIPDHIRSIAANPPPPQDSPLACSCLRTVHDPATAQGPGSWAKVSWSRRVLAASP

57

12.3%

NZ_RYYV01000004.1:

Lanthipeptides: allorf_104699_104878_lanthipeptide

27

CLRTVHDPATAQ

38

CLRT HDP+

3

CLRTAHDPSIGH

14

allorf_388828_388947

Leader:

MGLWIRAFSPRVRPKAEASRGS

Core:

SAEGQGHAARPCPSGDP

Core with dehydrations:

DhaAEGQGHAARPCysPDhaGDP

antiSMASH-DB 4.0 matches for allorf_388828_388947

100.0%

NZ_LMEI01000006.1:

Lanthipeptides: allorf_388828_388947_lanthipeptide

1

SAEGQGHAARPCPSGDP

17

SAEGQGHAARPCPSGDP

1

SAEGQGHAARPCPSGDP

17

41.2%

NZ_CP041613.2:

Lanthipeptides: allorf_01202655_01202762_lanthipeptide

3

EGQGHAARPCPS

14

+G+GH ARPC +

1

DGEGH-ARPCSA

11

35.3%

NZ_CP070496.1:

Lanthipeptides: allorf_3774399_3774488_lanthipeptide

6

GHAARPCPSG

15

G RPC SG

4

GKVERPCRSG

13

with 2 more sequence matches

26.9%

NZ_SIMU01000019.1:

Lanthipeptides: allorf_19974_20192_lanthipeptide

1

SAEGQGHAARPCPSGD

16

S G H PCP G

4

SKRGSPHGTTPCPPGS

19

20.0%

NZ_CP019286.1:

Lanthipeptides: allorf_089957_090166_lanthipeptide

8

AARPCPSG

15

A RPCP G

5

AVRPCPRG

12

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.70 out of 23.79

Confidence: medium

Additional weak hits (11):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.35 out of 26.33

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.94 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.09 out of 29.25

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.94 out of 33.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.91 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.20 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.74 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.72 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.96 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.52 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.09 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

TFBS Finder

Core peptide predictions

ASC82_RS06140 - Class II

Cleavage pHMM score: -3.70

RODEO score: 0

Monoisotopic mass: 3141.4 Da

Molecular weight: 3143.4 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3161.5 Da N = 1; 3179.5 Da N = 2; 3197.5 Da N = 3

ASC82_RS30550 - Class II

Cleavage pHMM score: -1.30

RODEO score: 0

Monoisotopic mass: 2705.4 Da

Molecular weight: 2707.1 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 2725.1 Da N = 1; 2743.2 Da N = 2; 2761.2 Da N = 3; 2779.2 Da N = 4; 2797.2 Da N = 5; 2815.2 Da N = 6; 2833.3 Da N = 7

ASC82_RS06140 - Class II

Cleavage pHMM score: -3.70

RODEO score: 0

Monoisotopic mass: 3141.4 Da

Molecular weight: 3143.4 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3161.5 Da N = 1; 3179.5 Da N = 2; 3197.5 Da N = 3

ASC82_RS30550 - Class II

Cleavage pHMM score: -1.30

RODEO score: 0

Monoisotopic mass: 2705.4 Da

Molecular weight: 2707.1 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 2725.1 Da N = 1; 2743.2 Da N = 2; 2761.2 Da N = 3; 2779.2 Da N = 4; 2797.2 Da N = 5; 2815.2 Da N = 6; 2833.3 Da N = 7

ASC82_RS06200 - Class II

Cleavage pHMM score: -1.80

RODEO score: 0

Monoisotopic mass: 1999.9 Da

Molecular weight: 2001.2 Da

Number of bridges: 1

allorf_383679_383804 - Class II

Cleavage pHMM score: -4.50

RODEO score: 0

Monoisotopic mass: 1496.8 Da

Molecular weight: 1497.8 Da

Number of bridges: 1

allorf_385739_385885 - Class II

Cleavage pHMM score: -5.70

RODEO score: 0

Monoisotopic mass: 2671.4 Da

Molecular weight: 2673.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2691.0 Da N = 1; 2709.0 Da N = 2

allorf_386141_386362 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 5919.1 Da

Molecular weight: 5922.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 5940.6 Da N = 1; 5958.6 Da N = 2; 5976.7 Da N = 3; 5994.7 Da N = 4; 6012.7 Da N = 5; 6030.7 Da N = 6; 6048.7 Da N = 7

allorf_388828_388947 - Class II

Cleavage pHMM score: -4.80

RODEO score: 0

Monoisotopic mass: 1599.7 Da

Molecular weight: 1600.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1618.7 Da N = 1

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_LMEI01000006.1 - Region 3 - NRP-metallophore,NRPS

Download region GenBank file

Download region SVG

Location: 441,166 - 498,325 nt. (total: 57,160 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

ASC82_RS06445: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ASC82_RS06540: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

ASC82_RS06600: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

Transferases: formyltransferase kinase

ASC82_RS06535: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

ASC82_RS06590: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Lyases: hydratase

ASC82_RS06610: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase

ASC82_RS06550: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ASC82_RS06605: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: transport

ASC82_RS06435: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002424.3	0.97	NRPS	saccharochelin A, saccharochelin B, saccharochelin C, saccharochelin D, saccharochelin E, saccharochelin F, saccharochelin G, saccharochelin H	Saccharothrix sp.
BGC0002135.2	0.86	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.82	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.78	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001166.5	0.75	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.71	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001240.4	0.71	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002424.3	0.78	NRPS	saccharochelin A, saccharochelin B, saccharochelin C, saccharochelin D, saccharochelin E, saccharochelin F, saccharochelin G, saccharochelin H	Saccharothrix sp.
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001240.4	0.68	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 33.07 out of 36.53

Confidence: strong

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 28.45 out of 36.53

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 30.90 out of 36.53

Confidence: medium

Additional weak hits (16):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.24 out of 32.74

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.75 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.53 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.00 out of 26.06

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.41 out of 35.63

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.08 out of 33.14

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.12 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.70 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 26.25 out of 36.53

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.00 out of 18.57

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.60 out of 21.84

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.27 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.50 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 5, location 441165 - 498325:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Fo-OH-Orn - Ser - OH-Orn - OH-Orn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ASC82_RS06550: Fo-OH-Orn - Ser - OH-Orn - OH-Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (468..864): Fo-OH-Orn

nrpys: Fo-OH-Orn

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LTAAFDVTVWDMILVVGTEVVAYGLTEASVNSTL
Nearest Stachelhaus code(s):: Fo-OH-Orn DVWIIGAVNK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1514..1933): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFCSGEHNLYGPTEAAVDVTY
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (100% 8Å match); Ala or Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2696..3116): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: MNHVFDMSWEEWNVLLGGEYNLYGPTEYTINTLG
Nearest Stachelhaus code(s):: OH-Orn DMENLGLINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3783..4183): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LTAAFDVFAFEMILLVGTEICAYGLTEATVNSTL
Nearest Stachelhaus code(s):: OH-Orn DVFILGAVNK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

ASC82_RS06605: X

AMP-binding (102..529): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EKNSW-MLWNTSGVGAAGSLN--------VGCGG
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor

NZ_LMEI01000006.1 - Region 4 - RiPP-like

Download region GenBank file

Download region SVG

Location: 498,942 - 509,757 nt. (total: 10,816 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: electron transfer

ASC82_RS06645: flavodoxin

smcogs: SMCOG1134: flavodoxin

Hydrolases: protease

ASC82_RS06670: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000595.3	0.34	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000597.4	0.33	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0000842.5	0.28	other (non-nrp beta-lactam)	carbapenem MM4550	Streptomyces argenteolus
BGC0001882.3	0.28	PKS	chrysoxanthone A, chrysoxanthone B, chrysoxanthone C	Penicillium rubens Wisconsin 54-1255
BGC0002122.2	0.23	NRPS	gramicidin S	Aneurinibacillus migulanus
BGC0000981.3	0.23	NRPS, PKS	cylindrospermopsin	Aphanizomenon sp. 10E6
BGC0000245.5	0.21	PKS	medermycin	Streptomyces sp. AM-7161
BGC0002338.3	0.21	PKS	Sorangipyranone	Myxococcales bacterium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000595.3	0.34	ribosomal	SCO-2138	Streptomyces coelicolor A3(2)
BGC0000597.4	0.33	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0000842.5	0.28	other (non-nrp beta-lactam)	carbapenem MM4550	Streptomyces argenteolus
BGC0001882.3	0.28	PKS	chrysoxanthone A, chrysoxanthone B, chrysoxanthone C	Penicillium rubens Wisconsin 54-1255
BGC0002122.2	0.23	NRPS	gramicidin S	Aneurinibacillus migulanus
BGC0000981.3	0.23	NRPS, PKS	cylindrospermopsin	Aphanizomenon sp. 10E6
BGC0000245.5	0.21	PKS	medermycin	Streptomyces sp. AM-7161
BGC0002338.3	0.21	PKS	Sorangipyranone	Myxococcales bacterium

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.50 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.44 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.98 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.81 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_LMEI01000007.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 38,404 nt. (total: 38,404 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

ASC82_RS07345: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase sulfhydrylase

ASC82_RS07385: O-succinylhomoserine sulfhydrylase

smcogs: SMCOG1168: O-succinylhomoserine sulfhydrylase

ASC82_RS30580: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: synthase

ASC82_RS07410: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.73	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.64	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000094.5	0.61	PKS, saccharide	methymycin	Streptomyces venezuelae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.73	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.64	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000094.5	0.61	PKS, saccharide	methymycin	Streptomyces venezuelae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.17 out of 25.31

Confidence: medium

Additional weak hits (6):

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 18.78 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.50 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.35 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.75 out of 30.25

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.03 out of 19.26

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 38404:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal) + (ccmal) + (Me-ohmal)
Non-colinear ordering:: ASC82_RS30580 + ASC82_RS07345 + ASC82_RS07340

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ASC82_RS07340: mmal

PKS_AT (559..843): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.5
Ethylmalonyl-CoA: 174.7
Methoxymalonyl-CoA: 155.4
2-Methylbutyryl-CoA: 111.1
Propionyl-CoA: 100.1
trans-1,2-CPDA: 93.4
CHC-CoA: 75.3
Malonyl-CoA: 69.3
Isobutyryl-CoA: 40.4
Benzoyl-CoA: 28.8
Acetyl-CoA: 27.1
fatty_acid: 26.0
3-Methylbutyryl-CoA: 11.1
inactive: 10.8

ASC82_RS07345: mal

PKS_AT (929..1223): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.5
inactive: 112.9
Methoxymalonyl-CoA: 75.5
Methylmalonyl-CoA: 68.3
Isobutyryl-CoA: 41.9
Propionyl-CoA: 38.4
Ethylmalonyl-CoA: 31.0
2-Methylbutyryl-CoA: 29.7
Acetyl-CoA: 22.7
Benzoyl-CoA: 16.4
CHC-CoA: 15.4
fatty_acid: 12.0
trans-1,2-CPDA: 11.2
3-Methylbutyryl-CoA: 0.0

ASC82_RS30580: mmal - mmal

PKS_AT (0..156): mmal

ATSignature: (unknown)

No matches above 50%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 82.7
Ethylmalonyl-CoA: 56.5
Methoxymalonyl-CoA: 51.7
2-Methylbutyryl-CoA: 36.7
Propionyl-CoA: 34.1
Malonyl-CoA: 21.0
trans-1,2-CPDA: 18.8
Isobutyryl-CoA: 16.5
Acetyl-CoA: 13.5
fatty_acid: 10.2
inactive: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (877..1159): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.4
Ethylmalonyl-CoA: 181.2
Methoxymalonyl-CoA: 155.6
2-Methylbutyryl-CoA: 129.2
Propionyl-CoA: 107.1
trans-1,2-CPDA: 101.8
CHC-CoA: 87.6
Malonyl-CoA: 78.9
Isobutyryl-CoA: 43.5
fatty_acid: 34.4
Acetyl-CoA: 29.3
Benzoyl-CoA: 25.9
3-Methylbutyryl-CoA: 15.5
inactive: 9.8

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CatR	H2O2-responsive repressor

NZ_LMEI01000007.1 - Region 2 - melanin

Download region GenBank file

Download region SVG

Location: 66,538 - 76,951 nt. (total: 10,414 nt) Show pHMM detection rules used

melanin: melC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000910.3	0.80	other (other)	melanin	Streptomyces coelicolor A3(2)
BGC0000911.3	0.79	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000909.3	0.64	other (other)	melanin	Streptomyces avermitilis
BGC0000912.3	0.59	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000908.3	0.48	other (other)	melanin	Streptomyces avermitilis
BGC0000885.3	0.31	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000662.5	0.31	terpene	grixazone A	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000889.5	0.31	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344
BGC0001592.5	0.30	other (other)	ferroverdin, bagremycin	Streptomyces lunaelactis
BGC0001624.3	0.24	other (other)	iso-A82775C	Pestalotiopsis fici

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000910.3	0.80	other (other)	melanin	Streptomyces coelicolor A3(2)
BGC0000911.3	0.79	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000909.3	0.64	other (other)	melanin	Streptomyces avermitilis
BGC0000912.3	0.59	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000908.3	0.48	other (other)	melanin	Streptomyces avermitilis
BGC0000885.3	0.31	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000662.5	0.31	terpene	grixazone A	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000889.5	0.31	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344
BGC0001592.5	0.30	other (other)	ferroverdin, bagremycin	Streptomyces lunaelactis
BGC0001624.3	0.24	other (other)	iso-A82775C	Pestalotiopsis fici

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.22 out of 21.55

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.50 out of 30.22

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.27 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_LMEI01000007.1 - Region 3 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 342,483 - 363,547 nt. (total: 21,065 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase

ASC82_RS08930: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: synthetase

ASC82_RS08870: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.46	terpene	astallatene	Arabidopsis thaliana
BGC0000948.5	0.45	other (other)	α-galactosylceramide	Bacteroides fragilis NCTC 9343
BGC0000657.5	0.44	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000563.4	0.44	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0002400.2	0.43	terpene	boleracene	Brassica oleracea var. oleracea
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001910.4	0.38	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000664.5	0.38	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.46	terpene	astallatene	Arabidopsis thaliana
BGC0000948.5	0.45	other (other)	α-galactosylceramide	Bacteroides fragilis NCTC 9343
BGC0000657.5	0.44	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000563.4	0.44	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0002400.2	0.43	terpene	boleracene	Brassica oleracea var. oleracea
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001910.4	0.38	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000665.5	0.38	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000664.5	0.38	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

ASC82_RS08870: PT_FPPS_like

Domain 1 (66 - 349): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.86 out of 18.57

Confidence: medium

Additional weak hits (6):

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.69 out of 20.97

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.99 out of 30.97

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.52 out of 29.65

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.08 out of 36.53

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.27 out of 25.88

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.43 out of 32.93

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
ToxT	Regulator of MSHA biosynthesis

NZ_LMEI01000007.1 - Region 4 - other

Download region GenBank file

Download region SVG

Location: 536,532 - 577,638 nt. (total: 41,107 nt) Show pHMM detection rules used

other: (Neocarzinostat or bcpB or frbD or mitE or prnB or CaiA or bacilysin or orf2_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: glucanase glucosidase

ASC82_RS09725: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

ASC82_RS09735: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

ASC82_RS09740: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

ASC82_RS09815: Pullulanase, type I

smcogs: SMCOG1297: Pullulanase, type I

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000519.4	0.49	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002285.2	0.49	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001374.5	0.46	other (other)	thienodolin	Streptomyces albogriseolus
BGC0000503.5	0.44	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001285.5	0.44	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0000558.5	0.43	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000551.5	0.42	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.41	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000923.3	0.41	other (other)	paerucumarin	Pseudomonas aeruginosa PAO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000519.4	0.49	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002285.2	0.49	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001374.5	0.46	other (other)	thienodolin	Streptomyces albogriseolus
BGC0000503.5	0.44	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001285.5	0.44	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0000558.5	0.43	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000551.5	0.42	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.41	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000923.3	0.41	other (other)	paerucumarin	Pseudomonas aeruginosa PAO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.86 out of 25.31

Confidence: medium

Additional weak hits (5):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.67 out of 18.57

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.38 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.53 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.50 out of 21.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CatR	H2O2-responsive repressor

NZ_LMEI01000009.1 - Region 1 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 96,212 - 117,267 nt. (total: 21,056 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

ASC82_RS13925: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Hydrolases: hydrolase

ASC82_RS13915: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: synthase synthetase

ASC82_RS13950: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

ASC82_RS13960: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000244.4	0.46	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002073.3	0.41	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0000947.5	0.40	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0002531.3	0.39	NRPS	cepaciachelin	Burkholderia ambifaria AMMD
BGC0001364.3	0.39	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000943.5	0.38	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0001456.3	0.38	terpene	isorenieratene	Streptomyces argillaceus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000244.4	0.46	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002073.3	0.41	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0000947.5	0.40	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0002531.3	0.39	NRPS	cepaciachelin	Burkholderia ambifaria AMMD
BGC0001364.3	0.39	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000943.5	0.38	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0001456.3	0.38	terpene	isorenieratene	Streptomyces argillaceus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

ASC82_RS13950: PT_FPPS_like

Domain 1 (49 - 349): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.91 out of 28.19

Confidence: strong

Additional weak hits (11):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.70 out of 26.33

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.03 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.02 out of 28.14

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.62 out of 20.97

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 20.96 out of 36.53

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.34 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.49 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.84 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.83 out of 28.19

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.07 out of 32.93

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.59 out of 29.65

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
ZuR variant 1	Zinc-responsive repressor

NZ_LMEI01000009.1 - Region 2 - terpene

Download region GenBank file

Download region SVG

Location: 143,328 - 169,931 nt. (total: 26,604 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

ASC82_RS14220: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

ASC82_RS14260: dehydrogenase

smcogs: SMCOG1181: dehydrogenase

Transferases: aminotransferase transferase

ASC82_RS14195: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

ASC82_RS14265: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Ligases: synthetase

ASC82_RS14215: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000663.5	0.71	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0000647.3	0.58	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000656.5	0.50	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0002162.3	0.49	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0002610.3	0.49	terpene	stellatic acid	Aspergillus stellatus
BGC0000646.5	0.49	terpene	β-carotein	uncultured bacterium
BGC0000630.3	0.49	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000640.3	0.48	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0002508.2	0.48	other (other)	8-azaguanine	Streptomyces pathocidini

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000663.5	0.71	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0000647.3	0.58	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000656.5	0.50	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0002162.3	0.49	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0002610.3	0.49	terpene	stellatic acid	Aspergillus stellatus
BGC0000646.5	0.49	terpene	β-carotein	uncultured bacterium
BGC0000630.3	0.49	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000640.3	0.48	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0002508.2	0.48	other (other)	8-azaguanine	Streptomyces pathocidini

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

ASC82_RS14210: T2TS

Domain 1 (25 - 657): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

ASC82_RS14215: PT_FPPS_like

Domain 1 (73 - 365): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

ASC82_RS14225: PT_phytoene_like

Domain 1 (12 - 302): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → PSPP; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

ASC82_RS14230: PT_phytoene_like

Domain 1 (19 - 277): Prenyltransferase; Phytoene synthase like

Subtype: Hydroxysqualene synthase

Reactions:: PSPP → hydroxysqualene

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.97 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.45 out of 29.38

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.67 out of 20.97

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.54 out of 25.88

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.72 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description

NZ_LMEI01000009.1 - Region 3 - butyrolactone

Download region GenBank file

Download region SVG

Location: 518,893 - 529,879 nt. (total: 10,987 nt) Show pHMM detection rules used

butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

ASC82_RS15915: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Lyases: dehydratase

ASC82_RS15900: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000849.4	0.79	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000848.5	0.78	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000850.5	0.65	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0000244.4	0.52	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0002578.2	0.48	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0000783.3	0.41	saccharide	O-antigen	Xanthomonas oryzae pv. oryzae
BGC0000706.3	0.41	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000003.3	0.37	PKS	AF-toxin	Alternaria alternata
BGC0001458.4	0.36	other (aminocoumarin)	salinipostin G, Salinipostin G, Salinipostin A, Salinipostin B, Salinipostin C, Salinipostin D, Salinipostin E, Salinipostin F, Salinipostin H, Salinipostin I, Salinipostin J, Salinipostin K	Salinispora tropica CNB-440
BGC0000207.5	0.36	PKS	chartreusin	Streptomyces chartreusis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000849.4	0.79	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000848.5	0.78	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000850.5	0.65	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0000244.4	0.52	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0002578.2	0.48	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0000783.3	0.41	saccharide	O-antigen	Xanthomonas oryzae pv. oryzae
BGC0000706.3	0.41	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000003.3	0.37	PKS	AF-toxin	Alternaria alternata
BGC0001458.4	0.36	other (aminocoumarin)	salinipostin G, Salinipostin G, Salinipostin A, Salinipostin B, Salinipostin C, Salinipostin D, Salinipostin E, Salinipostin F, Salinipostin H, Salinipostin I, Salinipostin J, Salinipostin K	Salinispora tropica CNB-440
BGC0000207.5	0.36	PKS	chartreusin	Streptomyces chartreusis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.93 out of 24.78

Confidence: medium

Additional weak hits (5):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.67 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.49 out of 29.65

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.86 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.93 out of 29.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.29 out of 29.25

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
LexA variant 2	Repressor of DNA damage response

NZ_LMEI01000009.1 - Region 4 - RiPP-like

Download region GenBank file

Download region SVG

Location: 642,095 - 653,534 nt. (total: 11,440 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002298.2	0.34	ribosomal	lassomycin-like peptide	Sanguibacter keddieii DSM 10542
BGC0000541.5	0.33	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000351.5	0.33	NRPS	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0000565.5	0.31	ribosomal	goadsporin	Streptomyces sp. TP-A0584
BGC0002579.2	0.31	ribosomal	carnobacteriocin XY	Carnobacterium maltaromaticum
BGC0001928.2	0.31	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002490.2	0.29	other (other)	yersinopine	Yersinia pestis CO92
BGC0000502.5	0.29	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001421.4	0.29	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0002663.2	0.29	ribosomal	amyloliquecidin GF610	Bacillus amyloliquefaciens UMAF6639

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002298.2	0.34	ribosomal	lassomycin-like peptide	Sanguibacter keddieii DSM 10542
BGC0000541.5	0.33	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000351.5	0.33	NRPS	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0000565.5	0.31	ribosomal	goadsporin	Streptomyces sp. TP-A0584
BGC0002579.2	0.31	ribosomal	carnobacteriocin XY	Carnobacterium maltaromaticum
BGC0001928.2	0.31	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002490.2	0.29	other (other)	yersinopine	Yersinia pestis CO92
BGC0000502.5	0.29	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001421.4	0.29	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0002663.2	0.29	ribosomal	amyloliquecidin GF610	Bacillus amyloliquefaciens UMAF6639

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_LMEI01000009.1 - Region 5 - NI-siderophore

Download region GenBank file

Download region SVG

Location: 821,971 - 854,469 nt. (total: 32,499 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

ASC82_RS17185: Antibiotic biosynthesis monooxygenase

smcogs: SMCOG1258: Antibiotic biosynthesis monooxygenase

Transferases: aminotransferase

ASC82_RS17235: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Isomerases: mutase

ASC82_RS17180: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.55	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001713.5	0.54	other (other)	ochrobactin	Ochrobactrum anthropi
BGC0002469.2	0.54	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0002683.2	0.53	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0001572.3	0.52	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002470.2	0.52	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0000295.5	0.52	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0002633.2	0.52	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0002305.2	0.52	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000939.5	0.51	other (other)	aerobactin	Grimontia hollisae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.55	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001713.5	0.54	other (other)	ochrobactin	Ochrobactrum anthropi
BGC0002469.2	0.54	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0002683.2	0.53	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418
BGC0001572.3	0.52	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002470.2	0.52	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0000295.5	0.52	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0002633.2	0.52	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0002305.2	0.52	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000939.5	0.51	other (other)	aerobactin	Grimontia hollisae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.93 out of 27.18

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.93 out of 24.78

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (7):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.11 out of 25.75

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.13 out of 30.97

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.24 out of 32.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.09 out of 29.65

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.77 out of 25.88

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.11 out of 23.43

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.88 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
LexA variant 2	Repressor of DNA damage response
NrdR	Represses ribonucleotide reductase encoding genes
VqsM	Regulator of quorum-sensing signalling systems

NZ_LMEI01000012.1 - Region 1 - NRPS-like

Download region GenBank file

Download region SVG

Location: 496,555 - 540,253 nt. (total: 43,699 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

ASC82_RS24460: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ASC82_RS24505: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Transferases: kinase methyltransferase sulfhydrylase

ASC82_RS24600: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

ASC82_RS24630: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

ASC82_RS24645: O-succinylhomoserine sulfhydrylase

smcogs: SMCOG1168: O-succinylhomoserine sulfhydrylase

Hydrolases: esterase hydrolase

ASC82_RS24470: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

ASC82_RS24575: thioesterase

smcogs: SMCOG1004: thioesterase

Translocases: permease

ASC82_RS24530: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.62	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.62	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002075.3	0.62	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.61	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.62	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.62	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002075.3	0.62	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.61	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.21 out of 20.47

Confidence: medium

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 30.57 out of 32.38

Confidence: strong

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 28.06 out of 32.93

Confidence: medium

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 29.15 out of 32.38

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (7):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.93 out of 27.78

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.96 out of 26.33

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.22 out of 26.06

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 25.37 out of 32.93

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.47 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.09 out of 28.19

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 21.30 out of 32.38

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 496554 - 540253:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gln)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ASC82_RS24575: Gln

Search NORINE for peptide: strict or relaxed

AMP-binding (9..399): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QWMTFDAAQWEAQGYSGGEINLYGPTECTINASS
Nearest Stachelhaus code(s):: Gln DAWQFGLINK (85% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CsoR	Copper-responsive repressor
RicR	Copper-responsive regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_LMEI01000013.1 - Region 1 - NRPS,NRPS-like

Download region GenBank file

Download region SVG

Location: 341,342 - 390,441 nt. (total: 49,100 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

ASC82_RS27145: dehydrogenase

smcogs: SMCOG1072: dehydrogenase

Transferases: PPTase

ASC82_RS27090: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Isomerases: mutase

ASC82_RS27160: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase synthase

ASC82_RS27095: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

ASC82_RS27190: cobaltochelatase subunit CobN

smcogs: SMCOG1263: cobaltochelatase subunit CobN

ASC82_RS29740: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ASC82_RS31290: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: permease

ASC82_RS27135: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.92	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.87	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001249.5	0.85	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.83	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.83	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.82	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.81	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.80	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001166.5	0.78	NRPS	HC-toxin	Alternaria jesenskae
BGC0001050.5	0.73	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.76	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.74	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001249.5	0.74	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001050.5	0.69	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.35 out of 20.47

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.19 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 26.07 out of 30.12

Confidence: medium

Additional weak hits (12):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.02 out of 30.22

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.50 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.29 out of 30.25

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.38 out of 26.85

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.38 out of 26.85

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.20 out of 30.22

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.73 out of 19.26

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.96 out of 26.33

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.61 out of 26.33

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.11 out of 30.22

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.49 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.49 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 341341 - 390441:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 341341 - 383669:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 343781 - 390441:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ASC82_RS29740: X

AMP-binding (174..572): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: REMAFDAAAEQPELVLGGEYNTYGPTETTITATS
Nearest Stachelhaus code(s):: Lys DAESVGTIIK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

ASC82_RS31290: Val - X

Search NORINE for peptide: strict or relaxed

AMP-binding (266..666): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LNNCFDVFTWEGWLMYGGDVNGYGPTEAAVFATC
Nearest Stachelhaus code(s):: Val DALWMGGVFK (53% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1328..1728): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YFLVYDASVREGSCLLSGEVNHYGPTETTMTAVF
Nearest Stachelhaus code(s):: Glu DPRSLSQMAK (59% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
OsdR	Development and stress management regulator
VqsM	Regulator of quorum-sensing signalling systems