LOCUS       NZ_LMEI01000009        11440 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces sp. Root431 contig_5, whole genome shotgun sequence.
ACCESSION   NZ_LMEI01000009
VERSION     NZ_LMEI01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root431
  ORGANISM  Streptomyces sp. Root431
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 925627)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 925627)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140605
            Assembly Name                     :: Root431
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426585.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 02:21:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,904
            CDSs (total)                      :: 5,829
            Genes (coding)                    :: 5,744
            CDSs (with protein)               :: 5,744
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 22 of 85
            Pseudo Genes (incomplete)         :: 72 of 85
            Pseudo Genes (internal stop)      :: 1 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:56:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 642094
            Orig. end                         :: 653534
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEI01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11440
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11440
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11440
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6440](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6440
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(328..1173)
                     /locus_tag="ASC82_RS30045"
                     /old_locus_tag="ASC82_15855"
     CDS             complement(328..1173)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477442.1"
                     /locus_tag="ASC82_RS30045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15855"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_056648009.1"
                     /transl_table=11
                     /translation="MRLERVGRRYGPRGPWVLREAVLDLPASALVRVVGTNGTGKSTLL
                     RLVAGIDAPSEGRVTGRPPRTAYVPERFPVALPFTATDYLVHLGRVQGLGRATARARAG
                     EWLERFGAAEHARTELAELSKGTGQKVAVAQALLADPDLLVLDEAWTGLDTTARAELDR
                     AVRERVAAGGTAVFVDHDPRRLAGEADAVYEVHSGAVRAVPAAVREAPREPRVRITASG
                     GKPLPEGLPGAPAAEPQDPDDATRPLVLTVDAAHSDALLAALLAASWHIHRLDSEGVRA
                     "
     gene            complement(1181..1858)
                     /locus_tag="ASC82_RS16470"
                     /old_locus_tag="ASC82_15860"
     CDS             complement(1181..1858)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153329.1"
                     /locus_tag="ASC82_RS16470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15860"
                     /product="AIM24 family protein"
                     /protein_id="WP_056648012.1"
                     /transl_table=11
                     /translation="MKSDLFAGEHLAEAAGFPGMTLQNAKSVKYTVNGEMHARQGSMVA
                     FRGDLQFERKGQGIGGLLKRAVTGEGLALMAVRGQGEAWFAHEAQNCFIIEIEQGDAFS
                     VNGRNVLCFDSTLHYEIKTVKGAGMTGGGIFNSVFSGYGKVALICEGSPIVIPVSPQAP
                     VYVDTDAVVGWSEQLRTSLHRSQSLGSMIRGGSGEAVQLKLEGQGFVVVRPSELTPQKN
                     SSN"
     misc_feature    complement(1939..1952)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            2030..2743
                     /locus_tag="ASC82_RS16475"
                     /old_locus_tag="ASC82_15865"
     CDS             2030..2743
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088892.1"
                     /locus_tag="ASC82_RS16475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15865"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_056648014.1"
                     /transl_table=11
                     /translation="MSSHETDVRDEKPQFVLPLVVRIERDAPPSRTDALETSARAVMEI
                     LTDDRSLGEGEWAQAMTDWQDARIRKVVRRARGAEWRKAGALPGITVTGEEAEVRVFPP
                     VPLDGWPKELAKLQVSGTDLDDPAEPGPVPDGAVMLWINPGLDMSAGKTMAQTGHGAQL
                     LWWAMRDADRKAWQEAGFPLAVRTATPGHWAALTAGDSGLPVVRDAGFTEIAPGKTVVS
                     EGSRLFAPHLRLRQP"
     gene            2756..3343
                     /locus_tag="ASC82_RS16480"
                     /old_locus_tag="ASC82_15870"
     CDS             2756..3343
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC82_RS16480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ASC82_15870"
                     /product="hypothetical protein"
                     /protein_id="WP_056648018.1"
                     /transl_table=11
                     /translation="MTSALGRRADRAASLLLLFLLAGADALARRPGRWKLRRIAGSDEV
                     RRKRQRAERELRARAEEIGRTGPWGPPLRTTLVDTCSRGGGRNFFDRNTPEQPALSCML
                     RLHLYFVVDGPAPPDIPASVARGELLSWDAPGDHARAEIPEPRRRRKAVLGTCTSDPAD
                     LTLTALRQRPGTLVAWALTEQYHTVPARRRAR"
     misc_feature    2768..2770
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3348..4001)
                     /locus_tag="ASC82_RS16485"
                     /old_locus_tag="ASC82_15875"
     CDS             complement(3348..4001)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677186.1"
                     /locus_tag="ASC82_RS16485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15875"
                     /product="ABATE domain-containing protein"
                     /protein_id="WP_056648021.1"
                     /transl_table=11
                     /translation="MSETPTAVSAPPPPAPGAEEYLALAFANSAVALPGGHFMDFLGTP
                     EAANRWLTEHGLAPADAGVREMCAAQLRSLREHLRSLLAARVEGLPALPSALAAVNDAL
                     SKAPTAALLYWDEKNGPYRAAPCPTDQILGRALATLAANAADLLTGPDADRLTRCGSAP
                     CNRYLLRHGRRHWCSTRCGDRARAARAYARRTRGQEDSSAQRDSSAQADSQEKD"
     gene            4087..4905
                     /locus_tag="ASC82_RS16490"
                     /old_locus_tag="ASC82_15880"
     CDS             4087..4905
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268528.1"
                     /locus_tag="ASC82_RS16490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15880"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_056648024.1"
                     /transl_table=11
                     /translation="MSDTGMPAEHEQIPVRVLGGPTAFFAYGGLRFLTDPTFDAPRDYR
                     APRGVLTKTAPSAATPAELGPIDVVLLSHDEHPDNLDASGRALLADVPLTLTTPGGAQR
                     LGAGAKGLADWESVVLDRPGGGTVTVTGVPALHGPGTREEVEPFTGQVVGFVLTGQDLP
                     TFYVSGDNASLGLVEEIAERFGPVDTAILFAGAPRFPQLFDGGLIVLDSAQAAEAATIL
                     GARRVVPVHCDSWAHFTESRDDVVAAFTDAGLLDRLDLGGALHRTPEPQM"
     gene            5001..6440
                     /locus_tag="ASC82_RS16495"
                     /old_locus_tag="ASC82_15885"
     CDS             5001..6440
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238120.1"
                     /locus_tag="ASC82_RS16495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15885"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_056648643.1"
                     /sec_met_domain="DUF692 (E-value: 7.7e-96, bitscore: 316.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAELGIGIGWRPEIAEAVEALPGLDWVEVVAENICADHLPDSLTR
                     LRERGVTVVPHGVSLGLGGADRPDPARLAALGEKAVALGAPLVTEHIAFVRAGGPLTAS
                     PLLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALIAWPGEELTEGQFLA
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAKALSELPVEAIAYVHVAGGVERDGVWHDTH
                     AHPVPRPVLDVLADLASRVSPPGVLLERDDDFPPAEELEAELEVIRETVIREAVAREAM
                     GASAAVPVGTAHQHGALSSAPAPRRAPLLIGAGARVSGGPMPSPVATTPAPTPAPAPAS
                     APDEGARQRVGLAQAALLSALVAGTPAPEGFDSRRLGVQSRALAAKRAGVVAKVAPELP
                     EILGKGFRPAFLAYARTRPMTGGYRRDALDFAEHLLLADRPEDPAARRRLTEWWQDRSG
                     SRPPGRAARLARAARHALVRR"
     gene            6437..7501
                     /locus_tag="ASC82_RS16500"
                     /old_locus_tag="ASC82_15890"
     CDS             6437..7501
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238121.1"
                     /locus_tag="ASC82_RS16500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15890"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_347336079.1"
                     /transl_table=11
                     /translation="MTVNALVVALYAAGIAGIALLITRVVRSHGGSAGPVHDRYEAAFL
                     NGGPARVADTAIAALQYDGRIAIGGPGIVAVVRPVAHDPVERAVLETHAAAPHGALHHL
                     RLGVMRHPAVQEIGDGLAARRLIEEPARRSTTLAWCLALGLTAFFLFPVSVVLTILDLS
                     TSPEMSVPFVVKVLPVLAAAGVTAIACGAISAKRITSAGQASLAAYGRAHGHLADAGHL
                     VALYGLRGLPDPVLREQLLAAARVYAPPRGRGRGRGRRPGAAHHLPTDYASAGVAAVWC
                     AGNTFCGGGGSSSGGGSGSCSSASCSSGSCSSGSCSGGGSGCGGGGGSSCSSSSGSSCS
                     SSSGSSCSSSSSSG"
     gene            7550..8305
                     /locus_tag="ASC82_RS16505"
                     /old_locus_tag="ASC82_15895"
     CDS             7550..8305
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238122.1"
                     /locus_tag="ASC82_RS16505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15895"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_056648029.1"
                     /transl_table=11
                     /translation="MVWVPLLLVACVVAGLGCARLFRASLMAALPASPSSGGDDAELTV
                     LEAAYLAGGPLRVTDLTLVAMHRERRLLLAHTGWATVVDADGGSRDEWESAVLGAIGPG
                     GQSPIPAVRPAVAAADAVRSLAQRLVDRGLAVSDAGRREVAAGVRAVRAAFLLTLVLGT
                     AAALLVPGAERTVVVAGFALPLLASGLCLLIGRIEVYPHTRWASPAGQRLLSGLSSADP
                     LTALATRGPAVLEPELRAALRGHAPVRLP"
     gene            8393..10027
                     /locus_tag="ASC82_RS16510"
                     /old_locus_tag="ASC82_15900"
     CDS             8393..10027
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-481). E-value:
                     3.3e-19. Score: 61.4. Matches aSDomain:
                     nrpspksdomains_ASC82_RS16510_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888542.1"
                     /locus_tag="ASC82_RS16510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15900"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_056648032.1"
                     /sec_met_domain="PF00561 (E-value: 9.5e-25, bitscore: 83.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSPALYGALSALLFTALPALSPAPAQDAPPSEARGTLLAARRAA
                     AEGIAFGSCPAAERLPPSVACGTVRVPLDYADPDGPLIPLTVSRVRASGAAADRQGALL
                     FNPGGPGASSTYFPLAAELPAWRRISAAYDLVGYAPRGVGRSAPVSCQDPADRVTGPTP
                     SPSRPSEVYKRERIARAKTYAEGCARRTGPALRHYHSLNNARDLEVVRAALGEPRLTYL
                     GGSYGTYIGGLYATLFPHRVRRMVLDAPVDPRPDRIWYHNNLAQSEAFERRWADFRTWV
                     AKHDVVYGLGGTAREVGARYERVRAELAARPAGGRVGPGQLHASMLDTGYDDRFWPLRA
                     GALAAYLKGDPQPLIAQAAPPETKETTETPGPDAGNGRAVYTAVECNDAPWPEEFAVWD
                     RDNTLLAERAPFETWDNAWMNLPCAYWKAPRQRPLDVRTGPGVLPPVLIMAAERDAATP
                     YAGALELQRRLSGSALVTERDAGTHGIAGGPNACVNGYLERYLLTGAVPAAEKGPEMTP
                     VRRASCAPHPEPDPVSLDGRAVKTPAV"
     aSDomain        8696..9835
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS16510_Abhydrolase_1.1"
                     /evalue="3.30E-19"
                     /label="ASC82_RS16510_Abhydrolase_1.1"
                     /locus_tag="ASC82_RS16510"
                     /protein_end="481"
                     /protein_start="101"
                     /score="61.4"
                     /tool="antismash"
                     /translation="ALLFNPGGPGASSTYFPLAAELPAWRRISAAYDLVGYAPRGVGRS
                     APVSCQDPADRVTGPTPSPSRPSEVYKRERIARAKTYAEGCARRTGPALRHYHSLNNAR
                     DLEVVRAALGEPRLTYLGGSYGTYIGGLYATLFPHRVRRMVLDAPVDPRPDRIWYHNNL
                     AQSEAFERRWADFRTWVAKHDVVYGLGGTAREVGARYERVRAELAARPAGGRVGPGQLH
                     ASMLDTGYDDRFWPLRAGALAAYLKGDPQPLIAQAAPPETKETTETPGPDAGNGRAVYT
                     AVECNDAPWPEEFAVWDRDNTLLAERAPFETWDNAWMNLPCAYWKAPRQRPLDVRTGPG
                     VLPPVLIMAAERDAATPYAGALELQRRLSGSALVTERDAG"
     gene            complement(10094..10807)
                     /gene="hemQ"
                     /locus_tag="ASC82_RS16515"
                     /old_locus_tag="ASC82_15905"
     CDS             complement(10094..10807)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="ASC82_RS16515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15905"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_024755095.1"
                     /transl_table=11
                     /translation="MSAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRAGYADE
                     VQELFDQLAAKDITVRGTYDLSGLRADADVMIWWHAETSDELQEAYSLFRRTKLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADENPRDYISVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKSVADLVAGLA"
ORIGIN
        1 ggtcgccgat cgcggtgaga ccgaggacgg cgagcacgcc gaggagcccc ggcagggcgg
       61 tcccggtgag gagcgccgcg aggtgggcgc ggctccggcc ggcggccgcg gcgacgacgt
      121 tccgggcggc ggccggttcc tgggtgaggc agatccgtac gcaccaggcc gtcaccggca
      181 ccaggcccgc cgtcgagaag ccgagcgcgc cgagcacggg ttcgcccgcg cggacgccga
      241 cggcgaggaa ggccgcgtag aggaggagcg gtgcgagcca gcgctgcgag cggagcagca
      301 gacccgcctg gtagcgcagg agcgccgtca tgcgcgtacg ccttcggagt cgagtcggtg
      361 aatgtgccag gaggcggcga ggagcgccgc gaggagcgcg tcggagtggg ccgcgtcgac
      421 ggtcaggacg agggggcggg tggcgtcgtc cgggtcctgc ggctcggcgg cgggggcgcc
      481 cggcaggccc tcggggagcg gcttgccgcc ggaggcggtg atccggaccc ggggctcccg
      541 cggcgcttcc cgcaccgctg ccggcacggc ccgtaccgca cccgaatgca cctcgtacac
      601 cgcgtccgcc tcccccgcga ggcgccgcgg gtcgtggtcg acgaagacgg ccgtgccacc
      661 cgccgcgacc cgctcccgga cggctcggtc cagttcggcg cgggcggtgg tgtcgagacc
      721 cgtccaggcc tcgtcgagga cgaggaggtc cgggtccgcg aggagggcct gggcgacggc
      781 gaccttctgg ccggtgccct tggagagctc cgccagttcc gtacgggcgt gctcggcggc
      841 cccgaagcgc tccagccact cccccgcgcg ggccctggcc gtcgcccggc ccaggccctg
      901 gacgcggccc aggtggacca ggtagtcggt ggccgtgaag ggcagggcca cggggaagcg
      961 ttcggggacg tacgccgtgc ggggcgggcg gccggtcacc cggccctcgc tcggggcgtc
     1021 gatcccggcg accagccgga gcagggtcga cttgccggtg ccgttggtgc cgacgacccg
     1081 gacgagggcg gaggcgggca ggtcgaggac ggcctcgcgc aggacccacg gtccgcgcgg
     1141 gccgtaccgg cgcccgaccc gctccagcct cactcgtccc tcaattcgac gagttcttct
     1201 gcggggtgag ctcgctgggc cggacgacca cgaagccctg cccctccagc ttgagctgca
     1261 ccgcctcgcc cgagccgccg cggatcatcg agccgaggga ctgcgagcgg tgcagcgagg
     1321 tgcggagctg ctcgctccag ccgacgacgg cgtcggtgtc gacgtagacg ggggcctgcg
     1381 gggagacggg gatgacgatg gggctgccct cgcagatcag cgccaccttc ccgtagccgg
     1441 agaagacgga gttgaagatg ccgccgccgg tcattccggc gcccttcacc gtcttgatct
     1501 cgtagtggag ggtggagtcg aagcagagga cgttgcggcc gttgaccgag aaggcgtcgc
     1561 cctgctcgat ctcgatgatg aagcagttct gcgcctcgtg cgcgaaccac gcctcgccct
     1621 ggccacgcac ggccatcagc gccaggccct cgccggtgac ggcgcgcttg aggaggccgc
     1681 cgatgccctg gcccttgcgc tcgaactgga ggtcgccgcg gaaggcgacc atcgagccct
     1741 gacgggcgtg catctctccg ttgacggtgt acttgaccga cttggcgttc tgcagggtca
     1801 tgcccgggaa gccggccgcc tcggcgaggt gctcacccgc gaaaagatca ctcttcatgc
     1861 ggggcatcct gtcccggaag ccctgattcc cacaagaatc ccctgccgac cacctcgtgg
     1921 tcccccgtcg accacctcgt gatccccgtc gacgaccccg tggcctcccc cgccgttccc
     1981 ccgccgacca ccctccggtc gtgcgtcacc gcagtggcag acttgccggg tgagcagcca
     2041 cgagaccgac gtccgcgatg agaagcccca gttcgtcctc cccctggtcg tacgaattga
     2101 gcgcgacgcg cccccgtccc gtaccgacgc cctggagacc tccgcgcggg ccgtcatgga
     2161 gatcctcacc gacgaccgtt ccctcggcga gggcgagtgg gcgcaggcca tgacggactg
     2221 gcaggacgcc cggatccgca aggtcgtgcg ccgggcgcgc ggcgcggagt ggcggaaggc
     2281 cggagcgctc ccggggatca cggtgaccgg cgaggaggcg gaggtacggg tcttcccgcc
     2341 ggtgccgctc gacggctggc ccaaggagct ggccaagctc caggtgtcgg gcaccgacct
     2401 cgacgacccg gccgagcccg gtcccgtacc ggacggggcc gtgatgctct ggatcaaccc
     2461 cgggctcgac atgtcggcgg gcaagacgat ggcgcagacc ggacacggcg cgcagctcct
     2521 gtggtgggcg atgcgggacg cggaccggaa ggcgtggcag gaggcgggct tcccgctcgc
     2581 ggtgcgcacc gcgacacccg ggcactgggc cgcgctcacg gcgggcgaca gcggactgcc
     2641 ggtcgtacgg gacgccggtt tcacggagat cgcgcccggg aagacggtcg tctccgaggg
     2701 aagccggctc ttcgctcccc acctgcggct gcgacagccg taggagaccg gggccatgac
     2761 ctctgcctta ggtcggcggg ccgaccgggc cgcgtccctg ctgctcctgt tcctcctggc
     2821 gggggccgac gccctcgccc ggcgccccgg caggtggaag ctcaggagga tcgccgggtc
     2881 ggacgaggtc cggcggaagc ggcagcgcgc cgagcgcgaa ctgcgggcgc gggccgagga
     2941 gatcggcagg accggaccgt ggggcccgcc cctgcgcacc acgctcgtgg acacgtgctc
     3001 ccggggcggg ggcaggaact tcttcgaccg gaacaccccg gagcagcccg ccctgtcctg
     3061 catgctgcgg ctgcacctgt acttcgtcgt ggacggtccc gcgccgcccg acatccccgc
     3121 gagcgtcgcc cgcggggagc tcttgtcgtg ggacgcaccc ggcgaccacg cgagggcgga
     3181 gatcccggaa ccgcgccgcc ggcgcaaggc ggtcctcggc acctgcacgt ccgaccccgc
     3241 cgacctcacc ctcacagcgc tgaggcagcg gccgggcacg ctcgtcgcgt gggcgctgac
     3301 cgagcagtac cacacggtcc cggcacggag acgagcccgg tgaaacgtca gtccttctcc
     3361 tgcgaatccg cctgcgccga cgaatccctc tgcgccgacg aatcctcctg cccccgcgtc
     3421 cggcgcgcgt aggcgcgggc cgcgcgggcg cggtcgccgc agcgggtgga gcaccagtgg
     3481 cggcggccgt ggcgcaggag gtagcggttg cagggggcgg agccgcatcg ggtgaggcgg
     3541 tcggcgtcgg ggccggtgag gaggtcggcc gcgttggccg cgagggtcgc gagggcgcgg
     3601 ccgaggatct ggtcggtggg gcagggcgcc gcgcggtagg gcccgttctt ctcgtcccag
     3661 tacagcagcg cggccgtggg ggccttgctc agggcgtcgt tgacggcggc gagcgccgag
     3721 ggcagggcgg gcagcccctc gacccgggcg gccagcagcg acctgaggtg ttcgcgcagg
     3781 gagcgcagtt gcgccgcgca catctcccgc accccggcgt cggccggcgc gaggccgtgc
     3841 tcggtgagcc accggttcgc ggcctcgggg gtgccgagga agtccatgaa gtgcccgccg
     3901 ggcagcgcga cggcgctgtt cgcgaaggcg agggcgaggt actcctcggc ccccggtgcg
     3961 ggcggcggcg gcgcggatac ggcggtcggg gtctcactca tggttctcat ggtacggctt
     4021 gccttcatcc gtgagacatg ggctatggtt ctcacggata gatcgcccac cattcgtgag
     4081 gttctgatgt ctgacaccgg catgcccgct gagcacgagc agatccccgt ccgcgtcctc
     4141 ggaggcccga ccgcgttctt cgcgtacggc ggcctgcgct tcctgaccga ccccacgttc
     4201 gacgcgcccc gcgactaccg ggcgccccgc ggcgtactga ccaagaccgc gccctccgcc
     4261 gccacgccgg ccgaactcgg cccgatagac gtggtcctgc tctcccacga cgagcacccc
     4321 gacaacctcg acgcgtccgg tcgcgccctc ctcgcggacg tcccgctgac cctgaccacg
     4381 cccgggggcg cgcagcgcct gggggcgggg gccaaggggc tggccgactg ggagtcggtc
     4441 gtcctggacc ggcccggcgg cggcaccgtc accgtcacgg gcgtgcccgc cctccacggt
     4501 cccggcaccc gcgaggaggt cgaaccgttc accggccagg tcgtcggctt cgtcctcacc
     4561 gggcaggacc tgcccacgtt ctacgtcagc ggcgacaacg cctcgctcgg cctggtcgag
     4621 gagatcgccg agcgcttcgg cccggtggac acggccatcc tgttcgccgg ggcgccgcgc
     4681 ttcccccagc tcttcgacgg cggcctgatc gtcctcgaca gcgcgcaggc cgccgaggcc
     4741 gccacgatcc tcggcgcccg ccgcgtcgtg cccgtccact gcgacagctg ggcgcacttc
     4801 accgagagcc gcgacgacgt ggtcgccgcc ttcaccgacg ccgggctgct cgaccgcctc
     4861 gacctgggcg gagccctcca ccggacgccg gaacctcaaa tgtagctctg aacgtccccc
     4921 tccttgggtt cgaacgtcgc cgccggggcc atcacgtgga tgactgagga ccgaaagagg
     4981 gggacgggga catgggggac atggcggaac tcggcatcgg catcggatgg cgccccgaga
     5041 tcgcggaggc ggtggaggcg ctgcccggcc tcgactgggt cgaggtggtc gcggagaaca
     5101 tctgcgccga ccacctgccc gactcgctga cccggctgcg ggagcgcggc gtgaccgtcg
     5161 tcccgcacgg agtgtcgctg ggactcggcg gcgcggaccg gccggacccg gcgcgcctcg
     5221 cggcgctcgg ggagaaggcg gtggcgctgg gggcgccgct ggtcacggag cacatcgcgt
     5281 tcgtacgggc cgggggcccg ctcaccgcgt cgccgctcct cgaagcgggc cacctgctgc
     5341 ccgtgccccg cacccgggac gccctcgacg tgctctgcga gaacgtgcgc atcgcgcagg
     5401 acgcgctgcc cgtaccgctc gccctggaga acatcgcggc cctcatcgcc tggcccgggg
     5461 aggagctgac cgagggacag ttcctggcgg agctcgtgga gcgtacgggg gtacggctcc
     5521 tcatcgacgt cgccaacctg cacaccaacc acgtcaaccg cggcgaggac ccggcgaagg
     5581 ccctgtccga actgccggtc gaggccatcg cgtacgtcca cgtggcgggc ggcgtcgagc
     5641 gcgacggcgt ctggcacgac acccacgccc acccggtccc gcgccccgtc ctcgacgtcc
     5701 tcgcggacct cgcctcgcgg gtctcgccgc ccggcgtgct cctggagcgg gacgacgact
     5761 tcccgccggc ggaggagctg gaggcggagc tggaggtgat ccgggagacg gtcatccggg
     5821 aggcggtggc ccgggaggcg atgggggctt cggcggcggt accggtgggc acggcccacc
     5881 agcacggcgc cctctcgtcg gcacccgcgc cccggcgggc gccgctgctc atcggtgccg
     5941 gtgcccgcgt ctcgggcggc ccgatgccga gccccgtcgc cacgacgccc gcacccacgc
     6001 ccgcacccgc acccgcatcc gcacccgacg aaggcgcacg ccagcgcgtc ggcctcgcgc
     6061 aggccgcgct gctgtcggcg ctcgtcgccg ggacgcccgc gcccgagggg ttcgacagcc
     6121 ggcgcctcgg ggtgcagagc cgggcgctcg cggccaagcg ggccggggtc gtcgcgaagg
     6181 tcgcccccga gctgccggag atcctcggca agggcttccg gcccgcgttc ctcgcgtacg
     6241 ccaggacccg ccccatgacc ggcggctacc gccgtgacgc cctcgacttc gccgagcacc
     6301 tgctgctcgc cgaccgtccc gaggacccgg ccgcccgacg gcggctcacc gagtggtggc
     6361 aggaccgttc cggcagccgc ccgcccggcc gcgccgcccg gctcgcccgc gccgcccgcc
     6421 acgccctcgt ccggaggtga ccgtgaacgc cctcgtcgtc gccctctacg cggccggcat
     6481 cgccggcatc gccctgctga tcacccgggt cgtccggtcg cacggcggct ccgccgggcc
     6541 ggtccacgac cggtacgagg cggccttcct gaacggcggc ccggcccggg tcgccgacac
     6601 cgccatcgcc gccctccagt acgacggccg gatcgcgatc ggcggccccg gcatcgtcgc
     6661 ggtcgtccgg cccgtcgcgc acgacccggt ggagcgcgcc gtgctggaga cgcacgcggc
     6721 ggccccgcac ggggccctgc accacctccg gctcggcgtg atgcgccacc cggcggtcca
     6781 ggagatcggt gacgggctcg cggcccgccg tctcatcgag gaaccggcgc ggcggagcac
     6841 caccctcgcc tggtgcctcg cgctcggtct cacggccttc ttcctcttcc ccgtctcggt
     6901 cgtcctcacg atcctcgacc tgtccacctc accggagatg tcggtgccct tcgtcgtgaa
     6961 ggtgctgccc gtcctcgccg ccgccggggt cacggccatc gcctgcgggg cgatctccgc
     7021 gaagcggatc acctcggcag gacaggcctc cctcgcggcg tacggccgcg cccacgggca
     7081 cctcgcggac gccggccacc tcgtggccct gtacggactc cgaggtctgc ccgacccggt
     7141 gctgcgggag caactcctcg ccgccgcccg ggtgtacgcg ccgccgcggg gacggggacg
     7201 cgggcgcgga cggcgtcccg gcgccgcaca tcacctcccc acggactacg cgtccgccgg
     7261 tgtggccgcg gtgtggtgcg cgggcaacac cttctgcggc gggggcggct cgtcgtccgg
     7321 cggaggatcc ggctcgtgct cgtccgcttc ctgttcctcc ggttcgtgct cctccggttc
     7381 gtgctcgggc ggcgggtccg gctgcggtgg cggaggcggc agttcctgtt cgagctccag
     7441 cggaagttca tgctccagtt ccagcgggag ttcatgctcc agctccagct ccagcggctg
     7501 atcacccgtt cgagtggcgg gcccgtcctt cctgtcatag aactcggaca tggtctgggt
     7561 tccgcttctc ctcgtggcat gcgtcgtggc cggtctcggc tgcgcgcgcc tgttccgggc
     7621 ctccctcatg gcggccctgc ccgcgtcccc ctcctccgga ggggacgacg ccgagctcac
     7681 cgtcctcgaa gccgcctacc tggccggcgg accgctccgg gtcaccgatc tgacgctcgt
     7741 cgcgatgcac cgggagcggc ggctgctgct cgcccacacc ggctgggcga cggtcgtgga
     7801 cgccgacggc ggcagccggg acgagtggga gagcgcggtg ctcggcgcca tcggtcccgg
     7861 cggccagtcc cccattcccg ccgtccgtcc ggcggtggcc gccgccgacg cggtgcgctc
     7921 gctcgcgcag cggctcgtcg accgggggct cgccgtgtcc gacgcgggcc gccgcgaggt
     7981 cgcggccggg gtgcgggcgg tgcgggccgc cttcctgctc accctcgtgc tcgggacggc
     8041 ggcggcgctc ctcgtgcccg gcgccgaacg gaccgtggtc gtcgccgggt tcgcactgcc
     8101 gctgctcgcc tccgggctct gcctgctgat cggccggatc gaggtgtacc cgcacacccg
     8161 ctgggcctcc cccgccgggc agcggctgct ctccggcctc tcctccgccg acccgctcac
     8221 ggccctcgcg acgcgcggcc ccgccgtcct ggagcccgag ctccgcgcgg cactccgggg
     8281 acacgctccc gtacgcctcc cgtagatcct ttacttcgcc cccgccccgc gccaacgatc
     8341 tcttcgtccc cgcccgcgcc ccacgcgcac gcggtcacga agggaagcac cgatgccctc
     8401 tcccgcgctg tacggagccc tcagcgccct cctgttcacc gccctccccg ccctctcccc
     8461 ggcccccgcg caggacgccc cgccgtccga ggcgcgcggc accctgctcg ccgcccgccg
     8521 ggcagccgcc gaggggatcg ccttcggctc ctgccccgcg gccgagaggc tgccgccgtc
     8581 cgtcgcgtgc ggcaccgtcc gcgtaccgct cgactacgcg gacccggacg ggcccctcat
     8641 cccgctgacc gtcagccgcg tccgggcctc gggggccgcc gccgaccggc agggcgcgct
     8701 cctcttcaac cccgggggcc cgggagcctc ctcgacgtac ttcccgctgg ccgccgagct
     8761 ccccgcgtgg cggcggatct ccgccgcgta cgacctcgtg ggctacgccc cgcgcggggt
     8821 gggccgctcc gcgccggtct cctgccagga cccggcggac cgggtgacgg gcccgacccc
     8881 gtcgccgagc cggccgtccg aggtgtacaa gcgggagcgg atcgcgcggg cgaagacgta
     8941 cgcggagggc tgcgcgcgcc ggacgggccc ggccctgcgc cactaccact cgctcaacaa
     9001 cgcccgggac ctggaggtcg ttcgggccgc gctcggcgag ccgcgcctca cgtacctggg
     9061 cggctcgtac ggcacgtaca tcggcggcct gtacgcgacc ctcttcccgc accgggtccg
     9121 gcggatggtc ctggacgcgc cggtggaccc gcggccggac cggatctggt accacaacaa
     9181 cctcgcccag tccgaggcgt tcgagcggcg ctgggcggac ttccgcacgt gggtggcgaa
     9241 gcacgacgtg gtgtacgggc tcggtggcac cgcacgggag gtgggcgcgc ggtacgagcg
     9301 ggtgcgcgcc gagctcgcgg cgcgcccggc gggcggccgg gtcggtcccg gccagttgca
     9361 cgcgtcgatg ctggacaccg gttacgacga ccggttctgg cccctgcggg cgggcgccct
     9421 cgccgcgtat ctgaagggcg atccgcaacc gctgatcgcg caggccgctc ccccggagac
     9481 gaaggagaca acggagacac cggggcccga cgccggcaac gggcgtgcgg tctacacggc
     9541 ggtggagtgc aacgacgcgc cctggccgga ggagttcgcc gtctgggacc gggacaacac
     9601 cctgctggcg gagcgggccc cgttcgagac ctgggacaac gcctggatga acctgccctg
     9661 cgcctactgg aaggcgcccc ggcagcggcc gctggacgtg cggacgggcc cgggggtgct
     9721 gccacccgtg ctgatcatgg cggcggagcg ggacgcggcg accccgtacg cgggagccct
     9781 cgaactccag cggcggctgt cgggctcggc cctggtgacc gagcgggacg cgggcacgca
     9841 cggaatcgcg ggcgggccga acgcctgcgt gaacggttat ctggagcgct atctgctcac
     9901 gggagcggta ccggccgccg aaaaaggccc ggaaatgacg ccggtgcggc gcgcatcatg
     9961 cgcgccgcac ccggagccgg acccggtgtc gctggacggg agggcagtga agacgcccgc
    10021 cgtctgatct tccgggtcaa ggggtggcgc gcccggccgg gagcagggcc gccaccaggt
    10081 tcgtacggcc gtgtcaggcc aggcccgcca ccaggtcggc gacgctcttg cggcgaccgg
    10141 tgtagaacgg gacctcctcg cggacgtgca tccgggcctc ggaggcgcgc aggtggcgca
    10201 tgaggtcgac gatgcggtgc agctcgtcgg cctcgaaggc gaggatccac tcgtagtcgc
    10261 ccagcgagaa ggaggcgacc gtgttggcgc ggacgtcggg gtagccgcgg gccatcttgc
    10321 cgtggtcggc gagcatgcgg cgacggtcct cgtccggcag caggtaccag tcgtaggagc
    10381 gcacgaaggg gtacaccgag atgtagtcgc gcgggttctc gtcggccagg aaggccggga
    10441 tgtgcgactt gttgaactcg gcggggcggt gcagcgccat gttggaccag accggctcca
    10501 gagcgcggcc cagcttggtg cggcggaaga ggctgtacgc ctcctggagc tcgtcgctcg
    10561 tctcggcgtg ccaccagatc atcacgtcgg cgtcggcgcg cagaccggac aggtcgtacg
    10621 tgccacggac ggtgatgtcc ttggcggcga gctggtcgaa gagctcctgg acctcgtcgg
    10681 cgtagccggc gcggtcctcg ggcagaacgt cgcgcagctt gaacacagac cacagggtgt
    10741 agcggatgac ctcgttgagg tccttcgcct tcttaccggc gttcgggatc ttttcgggcg
    10801 cactcatacg cctattctcg cccgtcacga ggagtgcccc gcaccggggt cggtcctttt
    10861 cgacgtggcg atgatctcgt ccccgaactc ggccgtgatc tcgtcggccg cgcggtgggc
    10921 cgaggcgatg cacgcgggga cgccgacgcc gtcgtaagcg gcgcccgcta cccgcagtcc
    10981 gggcagggcg gcgacggcct cgcggacgcg ggcgacccgg gcgagatggc cgacggggta
    11041 ctggggcagg ccgccggtcc agcgggtgac cgtggtggcg acggggcgcg cggtgagccc
    11101 ggtggcggcg gcgagatcct tgagggaggc ggcgacgagg tcggtgtcct cgcggtggac
    11161 ctgctcctct tctccgtgcc ggccgacgga ggtgcgcagc acgaagaggt cgggggcgcc
    11221 gtcggcgacc cagccccact tctggctgga gaaggtggag gccttgatcg tgtggccgtc
    11281 gaccggcggc acgaggaagc cggagccggc cagggcgggc atgtccgcgc ggcggaaggc
    11341 cagcgtgatc agggccatgg aggcgtacgc gatctccgcc agctcggcgg aggcggcggg
    11401 cgcgtgctcg gcgaggagcg cggcggcggg cccggcgggg
//
