LOCUS       NZ_LMEI01000009        10987 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces sp. Root431 contig_5, whole genome shotgun sequence.
ACCESSION   NZ_LMEI01000009
VERSION     NZ_LMEI01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root431
  ORGANISM  Streptomyces sp. Root431
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 925627)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 925627)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140605
            Assembly Name                     :: Root431
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426585.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 02:21:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,904
            CDSs (total)                      :: 5,829
            Genes (coding)                    :: 5,744
            CDSs (with protein)               :: 5,744
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 22 of 85
            Pseudo Genes (incomplete)         :: 72 of 85
            Pseudo Genes (internal stop)      :: 1 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:56:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 518892
            Orig. end                         :: 529879
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEI01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10987
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="3"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10987
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10987
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5987](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5987)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    533..548
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.67"
                     /tool="antismash"
     gene            complement(608..2164)
                     /locus_tag="ASC82_RS31395"
                     /old_locus_tag="ASC82_15275"
     CDS             complement(608..2164)
                     /EC_number="1.11.1.-"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF044997.1"
                     /locus_tag="ASC82_RS31395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15275"
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="WP_056647738.1"
                     /transl_table=11
                     /translation="MSHRSAAPAPAPTETAAARAPLTRRRVLTTSAAAAGVLALPGSAA
                     WAVPTSFEAETRTGRRAPGAVTDLSVVIRWNQAAMDAILGRYQAAGNRFGPATVNARAL
                     AIIHNAIYDAWACYDRCAVGSRFGGSLRRPRAERTLDNKNEAISYAAHLALLDLYPEYK
                     VAIDSMLRSLGHDPELTDPRRNSPAWIGRRTARAVLDYRHGDGANQLGTPAYSDTTGYQ
                     ARNAPQTAGAFDPSTVTDPAHWTPLIVNGKTQRYLTPQFAVMKPFALSRPDQFTVPAPP
                     AYPSVRMTAAIEELLDISANLTDEQKAIAEFWLNDDVTPPGAQQMWGRYVSARDGYGVD
                     EDAQLFFGLNMAECDAAIGSWAVKLDYDFARPSTLIPYDRRGQQIHSWGGPGQGTVAMD
                     GVDWQAYVAVPPFPATVSGHSTFSGAAAEFLRRFTGSDSFGDSYRFKAGASTVEPGLTP
                     RTDVVLHWPTFSEAARQAGISRVYGGMHWSFDNEPGIEMGHSIGKVVHRQAMRYFTGAH
                     R"
     misc_feature    complement(2221..2236)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.49"
                     /tool="antismash"
     gene            2531..3085
                     /locus_tag="ASC82_RS30640"
                     /old_locus_tag="ASC82_15280"
     CDS             2531..3085
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC82_RS30640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ASC82_15280"
                     /product="DoxX family protein"
                     /protein_id="WP_199862542.1"
                     /transl_table=11
                     /translation="MTVSPQVTPPATPVPTSPATQPSPPPAASPASATATASPPSVPTS
                     LGDRFERIAPRLHAHGLTMLRAGLGGVFVWFGVLKVIGYSPAAALVVAVLPFPAGEWFV
                     PALGWGEVALGLWLVSGRLRAVALPVLVAHLCGTFAVLVLTPGVAFAHANPLLLTLVGE
                     FVVKNVVLLAGAVVVTTRPRR"
     misc_feature    3091..3104
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.93"
                     /tool="antismash"
     gene            3314..3964
                     /locus_tag="ASC82_RS15895"
                     /old_locus_tag="ASC82_15285"
     CDS             3314..3964
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740923.1"
                     /locus_tag="ASC82_RS15895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15285"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_056647740.1"
                     /transl_table=11
                     /translation="MTKQERGTRTRDALIRSAAAEFDLHGYAMATLSAISLGAGVSPGA
                     LHFHFENKAAIAEAVEDAACTTLRRTARIAYRHRSNALQNLTDTTHAIARLFREDIVVR
                     AGFRLSCANDCRTELNLRQEWQSCVQQRLAEAADEGLLAAGTGRQQDLARTIVAATVGL
                     VTLCQDNGEWLSPHTVTGLWRTLLPTLTAPEALADLEPAGTAAVITRSLAPVG"
     gene            complement(4007..4975)
                     /locus_tag="ASC82_RS15900"
                     /old_locus_tag="ASC82_15290"
     CDS             complement(4007..4975)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (6-194). E-value: 1.1e-10.
                     Score: 33.0. Matches aSDomain:
                     nrpspksdomains_ASC82_RS15900_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037151.1"
                     /locus_tag="ASC82_RS15900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15290"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_056647743.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5.3e-10, bitscore:
                     34.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2e-08, bitscore:
                     29.4, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPRVLITGATGFIGRRVAATAAARADLGHLRLLVRSAVAGVTAG
                     EEPKAYGGPPTETVTGDLRDPDSLRRACEGVDVLIHCASAIGGDEELLHAVNDDGTRAL
                     VDAAVRAGVTRIVALSTASVHGRGPFRAAAPGTLPLAPGSATSRTRAAGERYVSDAGGA
                     VLRPHLVYGAGDRQLIPGLVRLLAALPGPLDAGASLHSVIDVDSLAGVLLGAALSPRTE
                     AGPYYVNHPEPVPVAELLAPCEELLLAGTDRPRAGAVDLAEAYDLLAGHPFGRHHLAML
                     ATDHWFADDRPWRDLGCAPGPGFTAGFPARASWYRDLLAGG"
     aSDomain        complement(4394..4957)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS15900_NAD_binding_4.1"
                     /evalue="1.10E-10"
                     /label="ASC82_RS15900_NAD_binding_4.1"
                     /locus_tag="ASC82_RS15900"
                     /protein_end="194"
                     /protein_start="6"
                     /score="33.0"
                     /tool="antismash"
                     /translation="ITGATGFIGRRVAATAAARADLGHLRLLVRSAVAGVTAGEEPKAY
                     GGPPTETVTGDLRDPDSLRRACEGVDVLIHCASAIGGDEELLHAVNDDGTRALVDAAVR
                     AGVTRIVALSTASVHGRGPFRAAAPGTLPLAPGSATSRTRAAGERYVSDAGGAVLRPHL
                     VYGAGDRQLIPGLVRLLAALPGPLD"
     CDS_motif       complement(4616..4672)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS15900_0001"
                     /evalue="3.40E+00"
                     /label="PKSI-KR_m1"
                     /locus_tag="ASC82_RS15900"
                     /protein_end="120"
                     /protein_start="101"
                     /score="0.5"
                     /tool="antismash"
                     /translation="RALVDAAVRAGVTRIVALS"
     gene            complement(5001..5987)
                     /locus_tag="ASC82_RS15905"
                     /old_locus_tag="ASC82_15295"
     CDS             complement(5001..5987)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037150.1"
                     /locus_tag="ASC82_RS15905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15295"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_056647745.1"
                     /sec_met_domain="AfsA (E-value: 1.4e-26, bitscore: 88.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQSDGGAVAHVPGEFVHRADPADIIPTGWTQLRENRFSVSASWP
                     AAHPFFSPVAGDRHDPVLVAETIRQVTMLVAHAELGVPADDQFVMWNLSYSVDADALAV
                     DGRASDVSIDVVVSDVKSSGNRLRSLRTTMVLTCEGRLLGTGGAVTSCTSAPAYRRIRG
                     ERMAALGRPVPLIPGVAPHLVGREDSKDVVLAPGTGPGQWQLRINTDHATLFRRPNDHV
                     PGMLLLEAARQAATMVLGSGAYLPKVMDVAFARYAELDSPCWIEAEIAPTSDPSVTTVR
                     VEGRQDDRSVFSCTLTSPSVSPSASPSASPSRDLAVAGGSLDSRLAG"
     misc_feature    complement(6055..6068)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     gene            6119..6802
                     /locus_tag="ASC82_RS15910"
                     /old_locus_tag="ASC82_15300"
     CDS             6119..6802
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037149.1"
                     /locus_tag="ASC82_RS15910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15300"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_056647747.1"
                     /transl_table=11
                     /translation="MAQQARAIRTRRAILNSAATVFAERGYERTTIGEILVRAGVTKGA
                     LYFHFASKEDLALGVLDAQMLDVPLTPPAIKIQELADQAFLLTHRLQRDPVVRASVALA
                     LDSGATSIDRVAPFQAWIDHLSDILTVAKARGELLVHVNVTDSATLFSGAFSGVQAMSQ
                     VMCDRQDLAHRVSVLLQHYMPSVCTPALLTSLELTAERAVTLGAEYDAILLRREQDQLA
                     DTPRQ"
     gene            6955..8106
                     /locus_tag="ASC82_RS15915"
                     /old_locus_tag="ASC82_15305"
     CDS             6955..8106
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037148.1"
                     /locus_tag="ASC82_RS15915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15305"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_106962390.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 3.2e-31, bitscore:
                     104.0, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.6e-29, bitscore:
                     97.7, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.5e-51, bitscore:
                     170.2, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGKDFDLYRPSEEHDMLRESVRALAEAKIAPFAAAVDEEGRFPQE
                     ALDALVANDLHAVHVPEAYGGAGADALATVIVIEEVARVCGSSSLIPAVNKLGSLPVIL
                     SGSEELKAKYLGPLAKGDAMFSYALSEPDAGSDAAGMKTRAVRDGDFWILNGVKRWITN
                     AGESEYYTVMAVTDPEKRSKGISAFVVEKSDEGVSFGAPEKKLGIKGSPTREVYFDNVR
                     IPADRMIGAEGTGFATAMKTLDHTRVTIAAQAIGIAQGALDYAKGYVKERKQFGKPIAD
                     FQGVQFMLADMAMKLEAARQLTYAAAAKSERVDGDLTFFGAAAKCFASDVAMEVTIDAV
                     QLLGGYGYTRDYPVERMMRDAKITQIYEGTNQVQRIVMARNLP"
     misc_feature    complement(8116..8130)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.93"
                     /tool="antismash"
     gene            8136..8921
                     /locus_tag="ASC82_RS15920"
                     /old_locus_tag="ASC82_15310"
     CDS             8136..8921
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262570.1"
                     /locus_tag="ASC82_RS15920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15310"
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="WP_056647750.1"
                     /transl_table=11
                     /translation="MTLKIAVCVKYVPDATGDRGFADDLTTDREAVDGLLSELDEYAVE
                     QALRISEESDDAEVTVVTVGPDDAKDALRKALSMGADKAVHVNDEDIHGSDVIATSAIL
                     AKALEKTGFDLVVCGMASTDGTMGVLPALLAERLNVPQVTLLSELSVEGELVKGRRDGD
                     SATELLEAPLPAVVSVTDQSGEARYPSFKGIMAAKKKPVEELDLDDLDIDADEVGLAGS
                     WTLVDAVAARPARTQGTIVTDEGDGGKQLAAFLATQKFI"
     gene            8961..9917
                     /locus_tag="ASC82_RS15925"
                     /old_locus_tag="ASC82_15315"
     CDS             8961..9917
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238294.1"
                     /locus_tag="ASC82_RS15925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_15315"
                     /product="electron transfer flavoprotein subunit alpha/FixB
                     family protein"
                     /protein_id="WP_056647754.1"
                     /transl_table=11
                     /translation="MAEILVLVDHADGAVRKPALELLTLARRIGEPSAVVLGAGEATAS
                     IAATAGEYGAATVYVADGAEFTDQLVVPKVDALTQLAKEKGAAAVLVTSSGEGKEIAAR
                     VALRLGSGLITDAVELEAGENGPVATQSVFAASYQVRSTVSHGVPVITVKPNSAAPEAA
                     PAAGTVENVAVTFTGNAAKVVARTPRVSTGRPELTESAIVVSGGRGVGAAEGFAVVEKL
                     ADSLGAAVGASRAAVDAGWYPHSNQVGQTGKQVSPQLYVAAGISGAIQHRAGMQTSKTI
                     VAVNKDPEAPIFDLVDYGVVGDLFEVLPQLTDEIGAR"
     gene            complement(9811..10410)
                     /locus_tag="ASC82_RS29530"
     CDS             complement(9811..10410)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF023594.4"
                     /locus_tag="ASC82_RS29530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="WP_347336077.1"
                     /transl_table=11
                     /translation="MPYLCPARSSRRVQGEGIVFHTGLRSGTAVQVEESAPDSELYFQR
                     CRWCHTTTFQRLLCPTCGSTDLSPELSGGEGVISVRRGVAAADADLWPVYMAEGFVVRC
                     RVDGPLHAVRPGVRVKLSSGVGVTGRRPVVRLCEEVPLDGWRVSGTECHSPAPYRWVTR
                     TVSGRRSRPSAAAVPRRGRRPRRSPRGRRSGPRGPC"
     misc_feature    10439..10453
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.29"
                     /tool="antismash"
ORIGIN
        1 ccaccagtac gccgggtact tgccgatgac gccgagccgc gaggcgatca ggcccaccag
       61 cacgaggagg acgctgccga cggcgagcag gacgaggaag cggacgggcg ccgggtcctg
      121 gaggacggcg aggacggcgg tggcggcggc cggggcgtgg acggcgcgca ggaggagcat
      181 gacggcgacg ctgaggccgg cggccacggc ggcgacccac agggagcggc cgaacagggc
      241 gacggcgacg aggccgagga tgccggagcc gagctggccg aggatcactc cgcggggctg
      301 cgccgggggc aggacgggcg tgctgcagat gatcatcgcg gtggccgcga gcggggcgat
      361 catcagcagg tgtccggtgg cctcgcccag ggccaccagg atcaccagcg acacgacggt
      421 ggcgagggtg ccgagggccg ccggccgggc gccggggaag ggcggggcgc cgcccgccca
      481 gcggctgcgg gcgggcgggg ccgccgcacc cgcgggggtc tgggtctggt tgctcacggt
      541 gttctctcct tggcggggct gggcggggcg ggcgcggggg cgccggggtg aggcgggggc
      601 agggctgtca gcggtgggcg ccggtgaagt accgcatggc ctgccggtgc acgaccttgc
      661 cgatgctgtg gcccatctcg atgccgggct cgttgtcgaa gctccagtgc atgccgccgt
      721 acacgcggga gatgccggcc tgccgggccg cctcactgaa ggtgggccag tgcaggacga
      781 cgtcggtgcg cggggtgagg ccgggctcga ccgtggaggc accggccttg aagcggtacg
      841 agtcgccgaa ggagtccgag ccggtgaagc ggcgcaggaa ctcggcggcc gcgccggaga
      901 aggtgctgtg cccggagacg gtcgccggga acggcggaac ggccacgtac gcctgccagt
      961 cgacgccgtc catggcgacg gtgccctggc cggggccgcc ccaggagtgg atctgctgcc
     1021 cgcgccggtc gtacgggatg agggtcgagg ggcgggcgaa gtcgtagtcg agcttgaccg
     1081 cccaggagcc gatggcggcg tcgcactcgg ccatgttgag gccgaagaac agctgggcgt
     1141 cctcgtcgac gccgtagccg tcgcgggcgg agacgtaccg tccccacatc tgctgggcgc
     1201 cgggcggggt gacgtcgtcg ttgagccaga actcggcgat cgccttctgt tcgtccgtca
     1261 gattggcgct gatgtcgagg agttcctcga tcgccgcggt catccggacc gaggggtacg
     1321 cgggcggggc gggcaccgtg aactggtcgg ggcggctcag cgcgaagggc ttcatcaccg
     1381 cgaactgcgg ggtgaggtag cgctgcgtct tgccgttcac gatcagcggg gtccagtggg
     1441 ccgggtccgt caccgtcgac gggtcgaacg cgccggcggt ctgcggggcg ttgcgggcct
     1501 ggtagccggt cgtgtcggag tacgcggggg tgccgagctg gttggcgccg tcgccgtgcc
     1561 ggtagtcgag gacggcccgc gcggtgcggc ggccgatcca cgcggggctg ttccggcggg
     1621 ggtcggtgag ctcggggtcg tggccgaggc tgcgcagcat cgagtcgatg gcgaccttgt
     1681 actcggggta caggtcgagc agcgcgaggt gggccgcgta gctgatcgcc tcgttcttgt
     1741 tgtcgagggt ccgctcggcg cggggcctgc gcagggagcc accgaagcgg ctgccgacgg
     1801 cgcagcggtc gtagcacgcc caggcgtcgt agatcgcgtt gtggatgatc gcgagggcgc
     1861 gggcgttgac ggtggcgggg ccgaagcggt tgccggcggc ctggtagcgg ccgagtatcg
     1921 cgtccatcgc ggcctggttc cagcggatga cgacactgag gtcggtgacg gcgccggggg
     1981 cgcggcggcc cgtgcgggtc tccgcctcga agctcgtggg gacggcccat gccgccgagc
     2041 cgggcagggc gagcaccccg gccgcggcgg cggacgtggt gagcacccgg cgccgggtga
     2101 gcggcgcgcg ggccgcggcg gtctcggtgg gtgcgggagc gggggcggcg gatctgtggg
     2161 acatccctgg attccttcgc atgatgccgg gaacggacgg acgcggtccg aagctgcgga
     2221 tcaaacacaa actcgaacag ttctcaggct ggcgagattt gatcgcgtcc ggcaacggtc
     2281 gatcgacttc ttgcatcgca ggtatgcaag gaagcgatgg gcgatgcggg ggtgtgcggg
     2341 acgtggttag cgtgaggcga cggcgtccgc ccctcctgcc gtgatttcgg cgcgatccga
     2401 gcggtccacc gcccgatcgc acccgactcg gcaccgcacg gcacccacgg cccaggcccg
     2461 cacggtggcc ccggcctcct ccgcccggca ccgccggccc cgcatcgact cgtgttggag
     2521 atgcccgcgc atgaccgttt ccccccaggt gacaccgccc gccacccccg tcccgacgtc
     2581 acccgccacg cagccttcgc ccccgcccgc cgcctcgccg gcctccgcca ccgccaccgc
     2641 ctcccctccc tccgtaccga cgtccctcgg cgatcgcttc gagcggatcg cgccacggct
     2701 gcacgcgcac ggcctgacca tgctccgggc cggtctcggc ggcgtgttcg tctggttcgg
     2761 cgtcctcaag gtcatcggct acagcccggc ggccgcgctc gtcgtcgccg tcctgccgtt
     2821 ccccgcgggc gagtggttcg tgcccgcgct cggctggggc gaggtggcgc tcggcctgtg
     2881 gctggtgtcc gggcgcctcc gggccgtggc cctgccggtg ctcgtcgccc atctgtgcgg
     2941 cactttcgcg gtcctcgtcc tcacgccggg cgtggccttc gcccacgcca acccgctgct
     3001 gctcaccctg gtgggcgagt tcgtcgtgaa gaacgtggta ctgctcgcgg gcgcggtggt
     3061 ggtgacgacc cgtccgcgcc gctgaatccg cgaacaaacc ttcccaacgg tttgttagca
     3121 accgcgctcg cgggcccgga acagcgcatg tccacggcct tcttgggtag atacacccgg
     3181 caacaccgaa gcgccctgga tcccaacgaa agctcgtaga ataccaatca gtcggtttaa
     3241 ggacgtcggg tggtacggcg cgacggggcg caaacccgtg ccgctgcgac gcatggtgct
     3301 acgggagccg ttggtgacca aacaagagcg gggcacacgg acccgcgacg cgctgatcag
     3361 gtcagcggcc gcagagttcg acctgcacgg ctacgccatg gccacgctgt ccgcgatcag
     3421 cctgggagcc ggcgtcagtc ccggggcgct ccacttccac ttcgagaaca aggcggcgat
     3481 cgccgaggcc gtcgaggacg cggcctgcac gaccctgcgg cggaccgccc ggatcgccta
     3541 ccgccaccgg tcgaacgccc tgcagaacct cacggacacc acgcacgcga tcgcccgcct
     3601 gttccgcgag gacatcgtcg tacgggcggg cttccggctc agctgcgcga acgactgccg
     3661 tacggagctc aatctgcgcc aggaatggca gagctgcgtc cagcaacggc tcgcggaggc
     3721 ggccgacgag ggcctcctcg ccgccggcac aggccgtcag caggacctcg cccgcacgat
     3781 cgtcgccgcc accgtcggcc tggtgaccct gtgtcaggac aacggcgagt ggctctcccc
     3841 ccacaccgtc accggcctgt ggcggactct gctgccgacc ctcacggctc ccgaggccct
     3901 cgcggacctg gaaccggcgg ggacggcggc cgtgatcacc cgctcgctcg ccccggtggg
     3961 gtgaggaccc gacgccaccc ctcggggagg gcgtcggccc gacggttcac ccgccggcga
     4021 ggagatcccg gtaccacgac gcgcgggccg ggaatcccgc ggtgaatccc ggacccggcg
     4081 cacagccgag gtcccgccac gggcggtcgt cggcgaacca gtggtcggtg gcgagcatgg
     4141 cgaggtggtg gcggccgaag gggtggcccg cgagcaggtc gtacgcctcg gccagatcca
     4201 ccgccccggc ccggggccgg tccgtccccg caagcaggag ctcctcacag ggcgcgagga
     4261 gctccgccac cggcaccggt tcgggatggt tgacgtagta cgggcccgcc tccgtacgcg
     4321 gcgagagcgc cgcgcccagc agcacgcccg ccaagctgtc cacgtcgatc acggagtgca
     4381 gcgaggcgcc ggcgtcgagc gggccgggca gggccgcgag cagccggacc aggccgggga
     4441 tcagctgccg gtcacccgcc ccgtacacga ggtggggtcg caggacggcc ccgcccgcgt
     4501 cgctcacgta ccgctcgccc gccgcccggg tgcggctggt cgccgagccg ggcgcgagcg
     4561 gcagggtgcc gggggcggcg gcgcggaagg ggccgcgacc gtggacggag gcggtgctca
     4621 gggcgacgat ccgggtcacc ccggcgcgta cggccgcgtc gaccagcgcc cgcgtcccgt
     4681 cgtcgttgac cgcgtggagc agctcctcgt ccccgccgat cgccgacgcg cagtggatca
     4741 gtacgtcgac cccttcgcag gcccggcgca gggagtcggg gtcgcggagg tcgccggtga
     4801 cggtctcggt cgggggaccg ccgtacgcct tgggctcctc ccccgccgtg acgccggcca
     4861 cggcggatcg gacgagcagc cggaggtgtc cgaggtccgc ccgtgccgcc gccgtcgccg
     4921 ccacgcgccg gccgatgaat ccggtcgccc cggtgatcag aacgcgaggg gccatggttc
     4981 gcgggccttc cttgtccggt tcagcccgcc aggcggctgt cgaggctgcc tccggccacc
     5041 gccaggtccc gggacgggga ggcggacggg gatgcggagg gggatacgga cggggacgtc
     5101 agcgtgcagg agaagacgga ccggtcgtcc tgacggccct cgacccggac ggtggtcacc
     5161 gaagggtcgg aggtgggcgc gatctcggcc tcgatccagc aggggctgtc gagttccgcg
     5221 tagcgcgcga aggcgacgtc catgaccttg gggaggtacg cgccgctccc gagcaccatc
     5281 gtcgccgcct gccgggcggc ctccaggagg agcattcccg gtacgtggtc gttcgggcgg
     5341 cggaagaggg tggcgtggtc cgtgttgatc cgcagctgcc actggcccgg tccggtgccc
     5401 ggggcgagga cgacatcctt ggagtcctct cggccgacca ggtgcggggc gactcccggt
     5461 atcagcggga ccggtcggcc gagcgcggcc atccgctcgc cccggatgcg gcggtacgcc
     5521 ggggcggagg tgcagctggt cacggcgccg ccggtaccga gcaggcggcc ttcgcaggtg
     5581 agcaccatgg tggtccgcag gctccggagc cggttgccac tgctcttcac gtcggagacg
     5641 acgacgtcga tgctgacgtc cgaggcgcgg ccgtcgaccg ccagcgcgtc ggcgtcgaca
     5701 ctgtaggaga ggttccacat gacgaactga tcgtccgcgg gtacccccaa ttcggcatga
     5761 gcgacgagca tggtgacctg acgtatcgtc tcggccacca gtacggggtc gtgccggtcc
     5821 cctgccaccg gggagaagaa gggatgcgcg gcaggccagc tggcggagac ggagaaccgg
     5881 ttctcccgca gctgcgtcca gcccgtgggg atgatgtccg cgggatcggc tcggtgcacg
     5941 aactctccgg ggacgtgtgc caccgctccc ccatcgctct gactcatggt tccccctcgc
     6001 gtccccacgc tcctgggcgg attttccacc cagcctagaa tacgtacagc ccggttttct
     6061 tttcaatgcg tcacgtcaca cggtgagcac agacagcgag acagcaggag gcgccgcctt
     6121 ggcacaacaa gcgcgtgcaa tcagaacccg acgggcgatc ctgaattcgg ccgcgaccgt
     6181 tttcgcggag cgtggatacg agcgcacgac catcggggag atcctggtgc gcgccggggt
     6241 gaccaaggga gcgctgtact tccatttcgc ttccaaggaa gacctcgccc tcggcgtgct
     6301 cgacgcccag atgctcgacg taccgctgac cccgccggcg atcaagatcc aggagctggc
     6361 ggaccaggcc ttcctgctca cccaccggct gcagcgtgac cccgtggtgc gcgcgagcgt
     6421 cgccctggcc ctggacagcg gcgccaccag catcgaccgg gtcgccccgt tccaggcatg
     6481 gatcgatcac ctgtcggaca ttctgacggt cgcgaaggcg cggggggagc tcctggtcca
     6541 tgtgaacgtg acggactcgg ccacactgtt ctcgggcgcc ttctcgggag tccaggcgat
     6601 gtcccaggtc atgtgcgatc gccaggacct ggctcaccgg gtgtcggtcc tgctccagca
     6661 ctacatgccg agcgtctgta cgccggcgct tctgaccagt ctcgagctga ccgcggagcg
     6721 cgccgtgaca ctcggtgccg agtacgacgc gatcctgctc cgccgggaac aggaccagct
     6781 cgccgacacg ccccgccaat aaaaccgacc gaccggtcct ctgagcgccc cgacgcggaa
     6841 cagcgtcggg gcgctctggc atcgtggcac tgagtgtgtt ttgctgaggg tactcagtgc
     6901 cacaagcgtt catcacggcc acacgcggcc aagcgtctag gggagctcca catcatgggc
     6961 aaggacttcg acctgtatcg gccctccgag gagcacgaca tgctccggga gtcggtgcgt
     7021 gcgctggcgg aggcgaagat cgcgccgttc gccgcggcgg tggacgagga gggccggttc
     7081 ccgcaggagg cgctggacgc gctggtcgcc aacgacctgc acgccgtgca cgtccccgag
     7141 gcgtacggcg gcgcgggcgc cgacgcgctg gcgaccgtga tcgtgatcga ggaggtcgcg
     7201 cgcgtgtgcg gcagctcctc cctgatcccg gccgtgaaca agctgggctc cctgcccgtg
     7261 atcctgtccg gctccgagga gctgaaggcg aagtacctgg gcccgctggc caagggcgac
     7321 gcgatgttct cgtacgccct gtccgagccc gacgcgggct ccgacgcggc cggcatgaag
     7381 acccgtgcgg tccgtgacgg cgacttctgg atcctcaacg gcgtcaagcg gtggatcacc
     7441 aacgccggcg agtccgagta ctacacggtg atggccgtca ccgaccccga gaagcgctcc
     7501 aagggcatca gcgccttcgt cgtggagaag tccgacgagg gcgtctcctt cggcgcgccg
     7561 gagaagaagc tcggcatcaa gggctccccg acccgcgagg tctacttcga caacgtccgc
     7621 atccccgcgg accgcatgat cggcgccgag ggcaccggct tcgccaccgc gatgaagacc
     7681 ctcgaccaca cccgcgtcac catcgcggcg caggccatcg gcatcgccca gggcgccctc
     7741 gactacgcca agggctacgt caaggagcgc aagcagttcg gcaagccgat cgccgacttc
     7801 cagggcgtgc agttcatgct cgccgacatg gccatgaagc tcgaggccgc ccgccagctc
     7861 acctacgccg ccgcggcgaa gagcgagcgc gtcgacggcg acctgacctt cttcggcgcc
     7921 gcggccaagt gcttcgcctc cgacgtcgcc atggaggtca ccatcgacgc cgtccagctc
     7981 ctcggcggct acggctacac ccgtgactac cccgtcgagc gcatgatgcg cgacgccaag
     8041 atcacgcaga tctacgaagg caccaaccag gtccagcgca tcgtcatggc ccgcaacctc
     8101 ccgtaaccac cagccttttc gaaaggagac aggccgtgac cctgaagatc gctgtctgtg
     8161 tgaagtacgt ccccgacgcg accggcgacc gcggtttcgc cgacgacctg acgaccgacc
     8221 gcgaggccgt cgacggcctg ctctcggagc tggacgagta cgcggtcgag caggctctgc
     8281 gcatctccga ggagtccgac gacgccgagg tcaccgtcgt gaccgtgggc ccggacgacg
     8341 ccaaggacgc gctgcgcaag gcgctgtcga tgggtgccga caaggccgtc cacgtcaacg
     8401 acgaggacat ccacggctcc gacgtcatcg ccacctccgc gatcctggcc aaggccctgg
     8461 agaagaccgg cttcgacctg gtcgtctgcg gcatggcctc gaccgacggc accatgggcg
     8521 tcctgccggc cctgctggcc gagcggctga acgtcccgca ggtcaccctc ctgtcggagc
     8581 tctccgtcga gggcgagctg gtgaagggcc gccgcgacgg cgactccgcc accgagctcc
     8641 tggaggcccc gctgccggcc gtcgtctcgg tcaccgacca gtccggcgag gcccgttacc
     8701 cctccttcaa ggggatcatg gccgccaaga agaagccggt cgaggagctg gacctggacg
     8761 acctggacat cgacgccgac gaggtgggcc tcgccggctc ctggaccctc gtcgacgcgg
     8821 tggccgcgcg tccggcccgt acgcagggca cgatcgtcac ggacgagggc gacggcggca
     8881 agcagctcgc cgccttcctc gccacccaga agttcatctg acccaccgtc agccagacac
     8941 ccactcagga gcaatgaatc atggctgaga tcctggttct cgtggaccac gccgacggtg
     9001 cggtccgcaa gccggccctc gaactcctca ccctggcccg ccgcatcggc gagccctcgg
     9061 cggtcgtcct cggcgccggc gaggccacgg cctccatcgc cgccacggcc ggcgagtacg
     9121 gcgccgccac cgtgtacgtc gcggacggcg ccgagttcac cgaccagctg gtcgtcccga
     9181 aggtcgacgc gctgacccag ctcgcgaagg agaagggggc cgccgccgtc ctggtgacct
     9241 cctccggcga gggcaaggag atcgccgccc gcgtcgcgct gcgcctcggc tccggtctga
     9301 tcaccgacgc cgtggagctg gaggccggcg agaacggccc ggtcgccacg cagtccgtct
     9361 tcgccgcctc gtaccaggtg cgctcgacgg tctcgcacgg cgtcccggtc atcaccgtca
     9421 agccgaactc cgccgccccg gaggccgccc cggccgccgg cacggtcgag aacgtcgccg
     9481 tcaccttcac cggcaacgcc gcgaaggtcg tcgcgcgcac cccgcgcgtc tccaccggcc
     9541 gccccgagct caccgagtcc gcgatcgtgg tctccggtgg ccgcggcgtc ggcgcggccg
     9601 agggcttcgc ggtcgtcgag aagctggccg actcgctcgg cgcggccgtc ggcgcctcgc
     9661 gcgccgccgt ggacgcgggc tggtacccgc actccaacca ggtcggccag accggcaagc
     9721 aggtctcgcc gcagctgtac gtcgcggcgg gcatctccgg cgcgatccag caccgggccg
     9781 gcatgcagac gtcgaagacg atcgtcgccg tcaacaagga ccccgaggcc ccgatcttcg
     9841 acctcgtgga ctacggcgtg gtcggcgacc tcttcgaggt actgccgcag ctgacggacg
     9901 agatcggcgc ccgctgaccg tcctcgtcac ccaacggtaa ggggcgggtg agtggcactc
     9961 tgtgccactc acccgccagc cgtcgagcgg gacctcctcg cagagccgca cgaccggcct
    10021 gcgaccggtc acgccgacgc ccgaggagag cttgacccgg actcccgggc gcaccgcgtg
    10081 cagcggcccg tcgacgcggc agcggaccac gaagccctcc gccatgtaga ccggccagag
    10141 gtcggcgtcg gccgcggcga ccccgcgccg tacggagatc acgccctcgc ccccgctcag
    10201 ttcgggcgag aggtccgtcg acccgcaggt cggacagagc aggcgctgga acgtcgtggt
    10261 gtggcaccag cggcagcgct ggaagtagag ctcggagtcg ggggccgact cctccacctg
    10321 gacggcggtc ccgctcctca gtcccgtgtg gaacacgatg ccttctcctt gcactcggcg
    10381 gctcgaccgt gcagggcaaa gatatggcac tgagtgccat caaataaaga cctgagttcc
    10441 ctcaattcga gataccgaga accccgaggg ttctcggcac cacccctcag ctctcaccga
    10501 ggctcgactc cacctcgcgc accacgcgcc acatgggagt ggaccgcttg gtgatgacca
    10561 cgacgacgtc ctccgtctcc tccgccggcg cgtccgcgac cgcgtccggg ctccaggtgg
    10621 cgcggacgaa ctccagggcg cggtcgacct ccgccagcgg gtcgccctcg cctccggagc
    10681 gcagccagtg gcgcagggcg tggttgtggg cggcgacgac ggccgccgcg accacgttgg
    10741 cgcgcagggt gccgtccctg cggtcggccc agcgggtgcg cagatagtcg ccgagggtct
    10801 tctcgtaccg ccagacgacc gagagctcgt acgtccgcag cccggtcacc tcgcgggtga
    10861 gccggtagcg ctggacggag aaggtggggt tctcggcgta catccgcatg acgagccggg
    10921 cggcgtcgca gacgcgcacg atcgggtcgt cggcgtcggc gctcgcggcg aggaagcggg
    10981 tcatgtc
//
