LOCUS       NZ_LMEI01000007        10414 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces sp. Root431 contig_3, whole genome shotgun sequence.
ACCESSION   NZ_LMEI01000007
VERSION     NZ_LMEI01000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root431
  ORGANISM  Streptomyces sp. Root431
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 876475)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 876475)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140605
            Assembly Name                     :: Root431
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426585.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 02:21:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,904
            CDSs (total)                      :: 5,829
            Genes (coding)                    :: 5,744
            CDSs (with protein)               :: 5,744
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 22 of 85
            Pseudo Genes (incomplete)         :: 72 of 85
            Pseudo Genes (internal stop)      :: 1 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:56:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 66537
            Orig. end                         :: 76951
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEI01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10414
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10414
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10414
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5414](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5414)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            434..1282
                     /locus_tag="ASC82_RS07570"
                     /old_locus_tag="ASC82_06960"
     CDS             434..1282
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660307.1"
                     /locus_tag="ASC82_RS07570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_06960"
                     /product="hypothetical protein"
                     /protein_id="WP_056643535.1"
                     /transl_table=11
                     /translation="MRPLATRRITALAISAAITLGAAGPAVAGEHHPSSAADRAAHAPL
                     PEAAALLTQAEALGELGSVTTPVTELVTAALKADAGQLPAADADALKAKIAAAVDQAKD
                     TAPAPLPETPAPDVPAPDLPAPDLPAPDLPAPDLPAPDLPAPDLPAPDLPVKPPVSLPV
                     AAGAKAAPGIAAPADVVDDALATVEKAVAALLAAVTSGDAGGVVPQVTATLTSLVNLAV
                     ATVLGSGLPAPDLAGLPALPALPVAVPELPVDPPELPVDTPVKPPVDVPDLPVKPPLPV
                     R"
     gene            1482..1751
                     /locus_tag="ASC82_RS07575"
                     /old_locus_tag="ASC82_06965"
     CDS             1482..1751
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239811.1"
                     /locus_tag="ASC82_RS07575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_06965"
                     /product="hypothetical protein"
                     /protein_id="WP_056643537.1"
                     /transl_table=11
                     /translation="MKSTKVAAVVAGSLVALGAATPAMAAAEALTPTSLNGGLEAIAAN
                     GLKTDMLSTTTEGSPLNTATDALGQLNEAGKGGSALLGGLPLGG"
     gene            1910..2887
                     /locus_tag="ASC82_RS07580"
                     /old_locus_tag="ASC82_06970"
     CDS             1910..2887
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015718.4"
                     /locus_tag="ASC82_RS07580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_06970"
                     /product="chaplin family protein"
                     /protein_id="WP_079039140.1"
                     /transl_table=11
                     /translation="MRSIISRKVLTAAAATGILSLTGGFALAANSHAGTGDSPGPGTGN
                     QVQAHLPADVCGKSMELGGFVAEAVGDLCAQAGEPGGYGDEPGEPTHPGEPTEPPTTPP
                     HTEPPQTEPPHTEPPTTPPHTEPPQTEPPHTEPPTTPPQTEPPHTEPPTTPPHTTPPPT
                     HPEEPGECEAPAPGEEPEPGCEEPTTPPTTPPTSPPWTPPTNPDEPGECEEPAPGEEPE
                     PGCEEPTTPPTTPPTSPPWTPPTNPDEPGECEEPAPGEEPEPGCEEPTTPPTDEPSHPT
                     TPPGPELPDTGSDPALMGAAAISAALIIGGTVVYLRGGRISRRH"
     gene            3006..3518
                     /locus_tag="ASC82_RS07585"
                     /old_locus_tag="ASC82_06975"
     CDS             3006..3518
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035837.1"
                     /locus_tag="ASC82_RS07585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_06975"
                     /product="hypothetical protein"
                     /protein_id="WP_056643542.1"
                     /transl_table=11
                     /translation="MTLRSHAGMGILMTALASAALAAPASAVEAPVIVPLQGLEPVLPM
                     DAPTVATGVPVPVPGAPTGFQKGLGSLPDVALPSVPLTGTLPKTVVDAPLPEVLDGSDL
                     GRALLSSPHSEMKAATPGAVVGNPVEAPRGNGLAGLPELTKPQVGLLTPVLSGALDPQL
                     GLAPSSH"
     gene            3805..4083
                     /locus_tag="ASC82_RS07590"
                     /old_locus_tag="ASC82_06980"
     CDS             3805..4083
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035838.1"
                     /locus_tag="ASC82_RS07590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_06980"
                     /product="chaplin"
                     /protein_id="WP_056643544.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVLAGTGAVALSGAGLAVADSGAEAVAAHSPGVASGN
                     VVQVPVHVPVNLCGNTVNVIGLLNPAFGNTCINADGGHDNGDGGYGG"
     gene            complement(4151..5017)
                     /locus_tag="ASC82_RS07595"
                     /old_locus_tag="ASC82_06985"
     CDS             complement(4151..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239814.1"
                     /locus_tag="ASC82_RS07595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_06985"
                     /product="tyrosinase family protein"
                     /protein_id="WP_056643547.1"
                     /transl_table=11
                     /translation="MVYTRKNQRDLTGAERRKFVAAVLELKRTGAYDEFVRTHIDHYSA
                     DGEGRLRTAHMAPSFLPWHRRFLLEFEKALRRIDPTVSVPYWDWTRDNSPASSIWGEDF
                     MGGNGRRADLQVMTGPFAHSTGRWNVTYAMSETRFLTRDFGRPRDPVALPTQAQLDEVM
                     RERVYDVAPWNSTSASGFRNRLEGWAPGQGNERFRIHNRVHRWVGGLMLGGGSVNDPVF
                     WLHHSFVDLVWSRWQQRNPGAAYLPAEPPPLGDSQYRKVVARHQPMAPWGVTPAELFDH
                     SRIYRYA"
     gene            complement(5001..5414)
                     /locus_tag="ASC82_RS07600"
                     /old_locus_tag="ASC82_06990"
     CDS             complement(5001..5414)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239815.1"
                     /locus_tag="ASC82_RS07600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_06990"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_056643549.1"
                     /sec_met_domain="melC (E-value: 4.2e-41, bitscore: 135.9,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVPRRVALRSLFALAVTAFTGGALGRIATRTPSGEPSGAPHDPL
                     AFDEMYGGRRILGFAGPGGAPVAFVDGRPLHLMRCADGGYVSPIDHYESCPTPLAATRA
                     AVDELGAARLSRFAAAHGDHTEPTGGSPRGVHA"
     gene            5564..6067
                     /locus_tag="ASC82_RS07605"
                     /old_locus_tag="ASC82_06995"
     CDS             5564..6067
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889043.1"
                     /locus_tag="ASC82_RS07605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_06995"
                     /product="DUF5949 family protein"
                     /protein_id="WP_221890723.1"
                     /transl_table=11
                     /translation="MTTSNVVSSARSASPLGTLTVIPWSSEPSADSAGTPFLMAYSLGD
                     GRDGPEAGQQAMRTALESMGLSVGDRLFDLDKDAGINATLLVEGGSVVLNLPFLKAQCP
                     VPDEWQAAARERGQVYLICSVRPWPEAVPGQPVDEARLRSFVSDEEMLAESAHVLMPVR
                     RLQG"
     gene            6096..6746
                     /locus_tag="ASC82_RS07610"
                     /old_locus_tag="ASC82_07000"
     CDS             6096..6746
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239817.1"
                     /locus_tag="ASC82_RS07610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_07000"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_056643553.1"
                     /transl_table=11
                     /translation="MATTDVRGGVPGQRQEESERETAGTGIGASRAFAWMLMITGAAGV
                     LSGWVITIDKFKLLEDPGFQPGCSLNPVVSCGNIMKSEQAGVFGFPNPMLGLVTYAVVV
                     AVGAGLLAGARYKGWLWLGLNAGMLFGVGFCTWLMYQSLYEINSLCLWCCLAWVATIVM
                     FWYVTAHNVRAGLLPAPAGVRTFLGEFAFALPVLHIGIIGMLILTRWWDFWTS"
     gene            6748..7776
                     /locus_tag="ASC82_RS07615"
                     /old_locus_tag="ASC82_07005"
     CDS             6748..7776
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239818.1"
                     /locus_tag="ASC82_RS07615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_07005"
                     /product="glycosyl hydrolase"
                     /protein_id="WP_056643555.1"
                     /transl_table=11
                     /translation="MNPFLLWPRAALVVLLAALLVLLLPHDGIAPGGRHTGSRAPVDPE
                     PLPGVPSGFFTGSDEAGVRRIAQVQAWLGGETLTVGHTYLPGDRWSNIEGHPALFEPWA
                     RWKEERPGRLFVLNVPLLDRNEAGLPDAEVRAGLRRGAGGAFDAHFEVLGKRLVEHGLG
                     DAVLVLGWEMNGTTYSHRCGPDPAAWKAYWRRVVGVLRDVEGQRFRFDFTPSRGLDAVA
                     WPLCYPGDDVVDIIGMDAYDQPAGLSFEEQVTEEYGLEHHVRFAAAHGKPVSYPEWGLF
                     RNGDNPVYVRGMLDWFAAHRPVYQTVTDYCPHGVWECADNPESARVVRGALGSPAGAPV
                     RP"
     gene            complement(7824..8528)
                     /locus_tag="ASC82_RS07620"
                     /old_locus_tag="ASC82_07010"
     CDS             complement(7824..8528)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239820.1"
                     /locus_tag="ASC82_RS07620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_07010"
                     /product="hemolysin III family protein"
                     /protein_id="WP_056643557.1"
                     /transl_table=11
                     /translation="MTAATPEVSPTDYPPSVENPPSLPTKPHLRGWLHAGMFPAVIVAG
                     LVLVALSDSPRARIACGIYVLTACLLFGISALYHRGNWGPRGEAILRRLDHANIFLIIA
                     GTYTPLTLLLLPDSTGEPLMWAVWAAAAAGIAFRVFWVGAPRWLYTPCYIAMGWAAVFF
                     LPDFMRAGGIAVLVLVIVGGLLYSAGGVIYGIKRPNPSPRWFGFHEVFHSLTLAAFIVH
                     YVGISLVAYQHA"
     misc_feature    complement(8612..8629)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.22"
                     /tool="antismash"
     misc_feature    8856..8871
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.27"
                     /tool="antismash"
     misc_feature    complement(9147..9162)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
ORIGIN
        1 tccatcgccg agggcgcggc cgtgaagtcc ccgggcgtcg gctccggcaa cgtcgtccag
       61 gtcccggtcc acatcccggt gaacgtctgc ggcaacaccg tgagcgtcat cggcgcgctg
      121 aacccgacct tcggcaactt ctgcgccaac gtctgatctc gcacagcgga tcggtccttc
      181 cgaagccggt gcgcccaccc cgatcggggt gggcgcaccg gctttttcct tgcggggact
      241 ggtgggctac tcgttcggcc gcaccgtcgt gcgccgttcg gtgcaacggc gtacgcccct
      301 tcggggggca ggccagaagg gtggattcac tcgaacggag acgtggcgct cagtagttcc
      361 ccggttgcgg agggctatac ccgcaggcac ggtgggagag aagttgtccg acgcataagg
      421 aaaaggaaca catatgcgtc ccctggctac acgccgcatc accgccctgg cgatatccgc
      481 cgccatcacc ctcggcgcgg cgggacccgc cgtcgccggc gagcaccatc cgtcctccgc
      541 ggccgaccgg gccgcccacg cgccactccc cgaagccgcc gccctgctga cccaggccga
      601 ggcgctcggt gagctcggct cggtgacgac ccccgtgacc gaactcgtca ccgcagccct
      661 caaggccgac gcgggccagc tgcccgcggc cgacgccgac gccctcaagg cgaagatcgc
      721 ggcggccgtc gaccaggcca aggacacggc cccggcaccc ctgccggaaa ccccggcacc
      781 ggacgtcccg gccccggacc ttcccgcgcc ggacctcccg gctccggatc tcccggctcc
      841 ggatcttccg gctccggatc tccccgcgcc tgatctcccc gcgccggatc tccccgtgaa
      901 gccgccggtg agtctgcccg tcgccgcagg cgcgaaggcc gcccccggta tcgccgcccc
      961 cgcggacgtc gtggacgacg ccctcgccac ggtcgagaag gccgtcgccg ccctgctcgc
     1021 cgccgtcacc tcgggcgacg ccggcggagt cgtaccgcaa gtgaccgcga ccctgacctc
     1081 gctggtcaac ctcgcggtcg ccaccgtgct cggcagcggt ctgcccgcgc cggacctggc
     1141 cggtctgccc gccctcccgg cgctcccggt cgcggttccc gagctgcccg tcgacccgcc
     1201 ggagctgccg gtcgacacgc ccgtgaagcc gcccgtcgac gtccccgacc tgccggtcaa
     1261 gcccccgctg cccgtcaggt agtcccccgc acaggcccgt accggcccgg cgtccgccgc
     1321 accggtacgg gccttcgcgt gcgacaaatg tcgagaaatc cttccttccg gagtttccgg
     1381 ggcagggcgc ctctcgttac aggagacgaa ggaacacatg ggattcggcc aagaagcccg
     1441 tgtgggacgt gacgtgaaag cacagaggaa ggattctccc catgaagtcc accaaggtcg
     1501 ccgcggtcgt cgccggttcg ctcgtcgcgc tgggcgccgc cacgcccgcc atggccgccg
     1561 cggaggcgct gacccccacc agcctgaacg gtgggctgga ggcgatcgcc gccaatggac
     1621 tgaagacgga catgctgagc accacgaccg agggctcccc gctgaacacc gccacggacg
     1681 cgctggggca gctcaacgag gccggcaagg gcggctcggc gctgctcggc ggactcccgc
     1741 tgggcggctg atcgaatgat tttctgatcg gccgccgtgt ggcccccgct cgacgcgggg
     1801 ccccgctccc tactgtctgg tccatccacg cgagcgtctg cgtggactgg ccggtcggag
     1861 cgagggggga tcctcgccgg tctctccctt tctccagagg aagagcggta tgcggtccat
     1921 catcagcagg aaagtactca ccgcagcggc agcgaccggc atcctgtcgc tgaccggcgg
     1981 tttcgcactt gccgccaact cccatgccgg tacgggcgat tcccccggtc ccggtacggg
     2041 gaaccaagtc caggcccacc tcccggcgga cgtttgcggc aagagcatgg aactcggcgg
     2101 attcgtcgcg gaagccgtcg gcgacctctg cgcccaggcg ggggaacccg gcggctacgg
     2161 cgacgagccg ggcgagccca ctcaccccgg cgagccgacg gagccgccga cgactccgcc
     2221 gcacaccgag ccgccccaga ccgagcctcc gcacacggag ccgccgacga ctccgccgca
     2281 caccgagccg ccccagacgg agcctccgca caccgagccg ccgacgactc cgccccagac
     2341 cgagccgccg cacacggagc cgccgacgac tccgccgcac accacgcctc cgccgaccca
     2401 tccggaggag cccggcgagt gcgaggcccc cgcccccggc gaggagccgg agcccggttg
     2461 tgaggagccg accactccgc cgaccactcc gcccacttcg ccgccgtgga cgccgccgac
     2521 caacccggac gagcccggtg agtgcgagga gccggcaccc ggtgaggagc cggagcccgg
     2581 ttgtgaggag ccgaccactc cgcccaccac cccgcccacc tctccgccgt ggacgccgcc
     2641 gaccaacccg gacgagcccg gtgagtgcga ggagcccgcg cccggtgagg agccggagcc
     2701 cggttgtgag gagccgacca ctccgccgac cgacgagccg agccacccga cgaccccgcc
     2761 gggtcccgaa ctccccgaca ccggcagcga tcccgcgctc atgggcgcgg ccgccatcag
     2821 tgccgcgctg atcatcggcg gcaccgtcgt ctacctccgg ggcggacgca tctcccgccg
     2881 tcactgaccc cacgggctga cggaacgtca agcgaggccc tcgccttcac cggtgagggc
     2941 ctccggcgtt gctgcacacg ggggtattcc gccacgtttc ccccgaacgc ctcgttcaac
     3001 agagcatgac tctgcgctct cacgccggca tgggaatcct catgaccgcg ctcgcctccg
     3061 ccgcactggc cgcaccggcc tccgccgtcg aggcgccggt gatcgtcccg ctccagggcc
     3121 tggagccggt gctcccgatg gacgccccca cggtggccac cggtgtgccc gtcccggtgc
     3181 ccggcgcgcc caccggcttc cagaagggcc tcggttccct gccggacgtc gccctgccca
     3241 gcgtgccgct caccggcacc ctcccgaaga ccgtggtgga tgcgccgctg ccggaggtcc
     3301 tggacggcag cgacctcggc cgggccctcc tcagcagccc gcactcggag atgaaggccg
     3361 ccacccccgg cgccgtcgtc ggcaacccgg tggaggcccc gcgcggcaac gggctcgccg
     3421 ggctgccgga gctgacgaag cctcaggtcg gcctgctcac ccccgtactg agcggagcac
     3481 tcgacccgca gctgggtctg gcgccgtcct cgcactgacg ccgtccggcc ccccgggcgg
     3541 gtgaagacac gattcttccc cgccacccca ggggtcgttg atcgttggaa gagaggctcg
     3601 tcgcatggcc gcggcccgtg gaccgaggag cgccccgtcg tatccggcgg cgggcgcctc
     3661 cggtccacgg gccattcctt tcggatcttt tcggatttct ttcggcccgt tcggcgcaat
     3721 gaccgtgacc agtccccgtg acgggcgttg aaggggagtc ggccgtgatt cctgcggtcg
     3781 ctttccacgt ccaaggagtt cttgatgtct cgcatcgcga aggctgccgc tgttctcgcc
     3841 ggcacgggtg ccgttgccct cagcggagcc ggcctggccg tcgccgactc gggcgccgag
     3901 gccgtcgccg cccactcgcc cggtgtcgcc tccggcaacg tcgtccaggt cccggtccac
     3961 gtcccggtca acctctgcgg caacaccgtc aacgtcatcg gcctgctcaa cccggccttc
     4021 ggcaacacct gcatcaacgc cgacggtggc cacgacaacg gtgacggcgg ctacggcggc
     4081 tgatcccccc gttccgacag gtgtgtcccc ccggccccag cgggccgggg ggacacacct
     4141 gtgtgcgggg tcaggcgtac cggtagatcc ggctgtggtc gaacagctcc gccggggtga
     4201 ccccccacgg cgccatcggc tggtgccggg cgacgacctt gcggtactgc gagtccccca
     4261 gcggcggcgg ttccgccggc aggtaggcgg ccccgggatt gcgctgctgc cagcgcgacc
     4321 agaccaggtc gacgaaggag tggtgcagcc agaagaccgg gtcgttgacg gagccgccgc
     4381 cgagcatcag cccgccgacc cagcggtgga cccggttgtg gatccggaac cgctcgttgc
     4441 cctggcccgg ggcccagccc tccaggcggt tgcggaagcc ggacgcgctc gtcgagttcc
     4501 agggcgccac gtcgtacacc cgctcccgca tcacctcgtc cagctgggcc tgcgtgggga
     4561 gcgccaccgg gtcgcggggc cggccgaagt ccctggtcag gaagcgggtc tcggacattg
     4621 cgtacgtgac gttccaccgc cccgtggagt gggcgaaggg gccggtcatg acctggaggt
     4681 cggcccggcg tccgttgccg cccatgaagt cctcgcccca gatcgacgag gccgggctgt
     4741 tgtcccgggt ccagtcccag tacgggacgg agaccgtcgg gtcgatccgg cgcagggcct
     4801 tctcgaactc cagcaggaac ctgcggtgcc agggcaggaa gctcggcgcc atgtgggcgg
     4861 tcctgagccg cccctccccg tcggccgagt agtggtcgat gtgggtgcgg acgaactcgt
     4921 cgtacgcgcc ggtccgcttg agctccagga cggcggcgac gaacttccgg cgctcggcgc
     4981 cggtcaggtc gcgctggttc ttacgcgtgt acaccacgcg ggctccctcc cgtcggttcg
     5041 gtgtggtcgc cgtgggcggc ggcgaaccgg gacagccggg ccgcccccag ctcgtcgacg
     5101 gcggcgcggg tggccgccag cggcgtcggg caggactcgt agtggtcgat cggcgagacg
     5161 tacccgccgt cggcgcagcg catcaggtgc agcggccggc cgtcgacgaa ggcgaccggg
     5221 gcgccgccgg gcccggcgaa gcccaggatg cggcggcccc cgtacatctc gtcgaaggcg
     5281 agcgggtcgt gcggtgcccc ggacggttcg cccgaagggg tgcgggtcgc gatgcggccg
     5341 agcgcaccgc cggtgaaggc ggtgacggcc agagcgaaga gggaccgcag tgcgacgcgg
     5401 cgcgggacga tcatgagtga tctcctcggg tcggcaattg ccctcagggc acaacgatga
     5461 aaacccgggt tcgttgcgtc ggaaatgcca gcggcgtgaa tatcgaccgt tcgggtgaaa
     5521 tcggcgcggg gaaagggtgg ttgggccact agcatccata ttcatgacca cttccaatgt
     5581 cgtttcgtcg gcgcgcagcg cctctccgct gggaactctc acggtgattc cgtggtccag
     5641 cgagccctcc gccgattcgg cgggcacgcc gttcctcatg gcgtattcgc tcggcgacgg
     5701 ccgggacggc ccggaggccg gtcagcaggc catgcggacg gccctggagt cgatgggcct
     5761 ctcggtcggc gaccggctct tcgacctcga caaggacgcc gggatcaacg ccacgctgct
     5821 cgtcgagggc ggctccgtcg tcctcaacct gccgttcctg aaggcgcagt gcccggtgcc
     5881 cgacgagtgg caggcggccg cccgcgagcg cggccaggtc tacctcatct gctccgtccg
     5941 gccctggccc gaggccgtcc ccgggcagcc ggtcgacgag gcgcggctgc gctccttcgt
     6001 ctccgacgag gagatgctcg ccgagagcgc ccacgtcctg atgccggtcc ggcgcctcca
     6061 gggctgagcg ggagagaacg gaagggggtc gcggcatggc gacgacggac gtacggggcg
     6121 gggtgcccgg gcagcggcag gaggagtccg agcgggagac cgcggggacg ggcatcggcg
     6181 cgagccgggc cttcgcctgg atgctgatga tcaccggggc ggcgggcgtg ctgtccggct
     6241 gggtgatcac gatcgacaag ttcaagctcc tggaggaccc cggcttccag cccgggtgca
     6301 gcctgaaccc ggtcgtgtcc tgcggcaaca tcatgaagag cgagcaggcg ggggtcttcg
     6361 gcttccccaa cccgatgctg gggctcgtca cgtacgccgt cgtcgtcgcc gtcggtgccg
     6421 ggctgctcgc cggcgcccgc tacaagggct ggctctggct cggcctcaac gccgggatgc
     6481 tcttcggggt cggcttctgc acctggctca tgtaccagtc gctgtacgag atcaactcgc
     6541 tctgcctgtg gtgctgcctc gcgtgggtcg ccaccatcgt catgttctgg tacgtgaccg
     6601 cgcacaacgt ccgggccggg ctgctgcccg cgccggccgg ggtgcggacc ttcctcggcg
     6661 agttcgcgtt cgccctgccc gtcctgcaca tcgggatcat cgggatgctg atcctgaccc
     6721 gctggtggga cttctggacc agctgacgtg aatcccttcc tgctctggcc gcgggccgca
     6781 ctcgtcgtgc tgctcgcggc ccttctcgtg ctcctgctgc cccacgacgg gatcgcgccc
     6841 ggcgggcggc acaccgggag ccgggccccg gtggaccccg agccgctgcc cggggtgccg
     6901 tccggcttct tcaccggatc cgacgaggcc ggggtgcggc ggatcgcgca ggtgcaggcc
     6961 tggctcggcg gcgagacgct gaccgtcggg cacacgtacc tccccggcga ccgctggtcc
     7021 aacatcgagg ggcatccggc gctcttcgag ccctgggcgc gctggaagga ggagcggccg
     7081 gggcggctct tcgtcctgaa cgtgccgctc ctcgaccgca acgaggcggg gctgcccgac
     7141 gcggaggtgc gcgcggggct gcggcgcggc gcgggaggcg ccttcgacgc gcacttcgag
     7201 gtcctgggga agcggctcgt ggagcacggg ctcggcgacg cggtcctggt gctcggctgg
     7261 gagatgaacg gcaccacgta cagccaccgc tgcggcccgg acccggcggc ctggaaggcg
     7321 tactggcggc gggtcgtggg ggtgctgcgg gacgtggagg ggcagcggtt ccgcttcgac
     7381 ttcaccccga gccgggggct cgacgccgtg gcgtggccgc tctgctaccc cggggacgac
     7441 gtcgtcgaca tcatcgggat ggacgcctac gaccagccgg ccgggctctc cttcgaggag
     7501 caggtgaccg aggagtacgg gctggagcac cacgtgcggt tcgcggcggc gcacgggaag
     7561 cccgtctcgt accccgagtg ggggctcttc aggaacgggg acaacccggt gtacgtgcgg
     7621 gggatgctcg actggttcgc cgcgcaccgg cccgtctacc agacggtgac ggactactgc
     7681 ccgcacggag tgtgggagtg cgcggacaat ccggagtcgg cgcgggtcgt caggggcgcg
     7741 ctggggtctc ccgccggcgc gcccgtacgc ccctgaggcc ctgaggtcct gaggcctgag
     7801 gtcctgaggg gcgtcgtgcc cggtcaggcg tgctggtagg ccaccagcga gatgccgacg
     7861 tagtggacga tgaaggccgc gagggtcagc gagtggaaga cctcgtggaa gccgaaccag
     7921 cggggtgagg ggttcgggcg cttgatgccg tagatgaccc cgcccgcgct gtagagcagg
     7981 ccgccgacga tgacgaggac gaggacggcg atgccgcccg cgcgcatgaa gtccggcagg
     8041 aagaagacgg cggcccagcc catcgcgatg tagcagggcg tgtacagcca gcgcggggca
     8101 ccgacccaga agacgcggaa ggcgatgccc gcggccgccg ccgcccacac cgcccacatg
     8161 agcggctcgc ccgtggagtc ggggagcagc agcagggtga gcggggtgta ggtgcccgcg
     8221 atgatcagga agatgttggc gtggtcgagg cggcgcagga tggcctcgcc gcgcggtccc
     8281 cagttcccgc ggtggtagag cgcgctgatg ccgaagagca ggcaggcggt gaggacgtag
     8341 atcccgcagg cgatccgggc gcggggcgag tcggagaggg cgacgaggac gaggcccgcg
     8401 acgatcacgg cggggaacat gccggcgtgc agccagcctc tgaggtgggg cttcgtcggg
     8461 agcgacgggg ggttctcgac cgacggcggg taatcggtcg ggctcacttc cggcgtggct
     8521 gcagtcatga caacgaatcg tacctacgcg tccgtaggtc ccggatatga gtggcgatgc
     8581 tcacgtgtgc ggccctctgg acatatgggc gttaacatcg gatgatcaaa tgagtgcggt
     8641 cggcaccgga tgagcagcta cgaagcatcc gggtcgcggc ccccacgggg catacaccaa
     8701 aaaaacccct caacaaggag caatcgtggc gcgcgacaac gcggctccca ccaccgtccc
     8761 gacccagcac caggagctcg tctcctgggt gaacgacatc gccgagatca cccagccgga
     8821 caacatcgtc tggtgcgacg gatccgaggc ggagtacgag cgcctgtgcg aggagctcgt
     8881 cgccaagggg accttcacga agctcgaccc ggtcaagcgc ccgaactcgt actacgccgc
     8941 ttccgacccc tccgacgtcg cgcgcgtcga ggaccgcacc tacatctgct ccgagaagga
     9001 ggaggacgcg ggcccgacca accactggaa ggccccggcc gagatgaagg agctcttcgc
     9061 gggctccgag ggcatcttcc gcggctccat gcgcggccgc accatgtacg tcgtgccctt
     9121 ctgcatgggc cccgtcggat ccccgctctc cgccatcggc gtcgagatca cggactccgc
     9181 gtacgtcgcc gtctccatgc gcaccatgac ccgcatggga caggccgtcc tggacgagct
     9241 cggcaccgac ggcttcttcg tcaaggccgt gcacacgctc ggcgcgccgc tcgccgaggg
     9301 cgaggccgac gtgccgtggc cctgcaacac caccaagtac atctcgcact tccccgagag
     9361 ccgcgagatc tggtcgtacg gctcgggcta cggcggcaac gccctgctcg gcaagaagtg
     9421 ctacgcgctc cgcatcgcct ccgtcatggc gcgtgacgag ggctggctcg ccgagcacat
     9481 gctgatcctc aagctcaccc cgccgcaggg cgagtcgaag tacgtcgccg ccgccttccc
     9541 gtcggcctgt ggcaagacca acctcgccat gctggagccc acgatctccg gctggacggt
     9601 cgagaccatc ggcgacgaca tcgcctggat gcgcttcggc gaggacggcc gcctctacgc
     9661 gatcaacccc gaggccggct tcttcggcgt cgcgcccggc accggcgagc acaccaacgc
     9721 caacgccatg aagaccatgt ggggcaactc cgtcttcacg aacgtcgccc tcacggacga
     9781 cggcgacgtg tggtgggagg ggatgaccga gacggccccc gcgcacctca cggactggaa
     9841 gggcaacgac tggacgcccg cgtcggagac gccggccgcc cacccgaacg cccgcttcac
     9901 cgtcccggcc gggcagtgcc cgatcatcgc gccggagtgg gaggacccga agggcgtgcc
     9961 gatctcggcc atcctcttcg gcggccgccg cgcctccgcc gtcccgctgg tgaccgagtc
    10021 cttcgactgg aaccacggcg tcttcctcgg tgcgaacgtc gcctccgaga agaccgccgc
    10081 cgccgagggc aaggtcggcg agctgcgccg cgaccccttc gccatgctgc cgttctgcgg
    10141 ctacaacatg ggcgactaca tggcccactg ggtcaaggtg ggcgcgaacg cggacgccgc
    10201 gaagctgccg aagatctact acgtgaactg gttccgcaag aacgacgcgg gcaagttcgt
    10261 gtggcccggc ttcggcgaga acagccgcgt cctgaagtgg atcgtggagc gcctggaggg
    10321 caaggccgag ggcgtcgaga ccccgatcgg catcctgccg accccggagt ccctcgacac
    10381 cgagggcctg gacctgccgg ccgaggacct ggag
//
