LOCUS       NZ_LMEI01000005         8159 bp    DNA     linear   CON 26-MAY-2024
DEFINITION  Streptomyces sp. Root431 contig_13, whole genome shotgun sequence.
ACCESSION   NZ_LMEI01000005
VERSION     NZ_LMEI01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root431
  ORGANISM  Streptomyces sp. Root431
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140605
            Assembly Name                     :: Root431
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426585.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 02:21:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,904
            CDSs (total)                      :: 5,829
            Genes (coding)                    :: 5,744
            CDSs (with protein)               :: 5,744
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 22 of 85
            Pseudo Genes (incomplete)         :: 72 of 85
            Pseudo Genes (internal stop)      :: 1 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:56:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8159
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEI01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..1108)
                     /NRPS_PKS="Domain: PKS_DHt (110-213). E-value: 3.3e-08.
                     Score: 25.6. Matches aSDomain:
                     nrpspksdomains_ASC82_RS30905_PKS_DHt.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060785.1"
                     /locus_tag="ASC82_RS30905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_29090"
                     /product="acyltransferase domain-containing protein"
                     /protein_id="WP_242427546.1"
                     /sec_met_domain="PKS_AT (E-value: 5.6e-38, bitscore: 119.1,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDPIQIQELANSCETDGIRARIIPVDYASHSAQVEIIEKELAEV
                     LAGLTPQVPQVPFFSTLEGGWITEPALDAAYWYRNLRHRVGFAPAVETLATHEGFTHFV
                     EVSAHPVLTMALPETVTGLGTLRREQGGQDRLVTSLAEAWANGLPVDWTSHLPATATHA
                     DLPTYAFQTERYWLQASAPTSTADDWRYRVDWKPLATAAEADLAGRWLVAAGGEPDAGL
                     LGALKAAGAEVEVVETGADDDREALAARLAGLTDGFTGVISLLDGLTPLVAWVQALGDA
                     GIEAPLWCVTRGAVSVGRHDTPADPDQAMLWGLGRVVALEHPERWAGLIDLPTKLDTST
                     LGHLVTVLAGSTGEDQIAIRTTGLHARRLA"
     gene            complement(<1..>1112)
                     /locus_tag="ASC82_RS30905"
                     /old_locus_tag="ASC82_29090"
     source          1..8159
                     /collection_date="2013"
                     /country="Germany: Cologne"
                     /db_xref="taxon:1736535"
                     /host="Arabidopsis thaliana"
                     /isolation_source="root"
                     /lat_lon="50.958 N 6.856 E"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. Root431"
                     /strain="Root431"
                     /submitter_seqid="contig_13"
     region          1..8159
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..8159
                     /SMILES="C(C)CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8159
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<1568:3462](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1569..3462)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        complement(468..776)
                     /aSDomain="PKS_DHt"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS30905_PKS_DHt.1"
                     /evalue="3.30E-08"
                     /label="ASC82_RS30905_PKS_DHt.1"
                     /locus_tag="ASC82_RS30905"
                     /protein_end="213"
                     /protein_start="110"
                     /score="25.6"
                     /tool="antismash"
                     /translation="VLTMALPETVTGLGTLRREQGGQDRLVTSLAEAWANGLPVDWTSH
                     LPATATHADLPTYAFQTERYWLQASAPTSTADDWRYRVDWKPLATAAEADLAGRWLVA"
     CDS_motif       complement(639..659)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30905_0004"
                     /evalue="1.80E+01"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="ASC82_RS30905"
                     /protein_end="156"
                     /protein_start="149"
                     /score="-0.4"
                     /tool="antismash"
                     /translation="VDWTSHL"
     CDS_motif       complement(771..800)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30905_0003"
                     /evalue="1.20E-03"
                     /label="PKSI-AT-mM_m9"
                     /locus_tag="ASC82_RS30905"
                     /protein_end="112"
                     /protein_start="102"
                     /score="11.3"
                     /tool="antismash"
                     /translation="FVEVSAHPVL"
     CDS_motif       complement(846..893)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30905_0002"
                     /evalue="1.30E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="ASC82_RS30905"
                     /protein_end="87"
                     /protein_start="71"
                     /score="17.2"
                     /tool="antismash"
                     /translation="LDAAYWYRNLRHRVGF"
     CDS_motif       complement(999..1031)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30905_0001"
                     /evalue="7.00E-05"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="ASC82_RS30905"
                     /protein_end="36"
                     /protein_start="25"
                     /score="15.3"
                     /tool="antismash"
                     /translation="VDYASHSAQVE"
     misc_feature    complement(1088..1101)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 17.82"
                     /tool="antismash"
     assembly_gap    1113..1133
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1134..1535)
                     /locus_tag="ASC82_RS30910"
                     /old_locus_tag="ASC82_29095"
     CDS             complement(<1134..1535)
                     /NRPS_PKS="Domain: PKS_AT (0-132). E-value: 8.8e-44. Score:
                     142.0. Matches aSDomain:
                     nrpspksdomains_ASC82_RS30910_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="ASC82_RS30910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_29095"
                     /product="acyltransferase domain-containing protein"
                     /protein_id="WP_277914772.1"
                     /sec_met_domain="PKS_AT (E-value: 5.9e-45, bitscore: 142.0,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGADLLDSSQEFGAAMAECETAFAPYVDWSLEAVVRQTPGAPSLE
                     RVDVVQPVTFAVMASLARVWQHYGVTPQAVIGHSQGEIAAAYVAGALTLDDAARVVALR
                     SQSIATHLAGKGGMISLALSESAALERLVD"
     aSDomain        complement(1140..1535)
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS30910_PKS_AT.1"
                     /evalue="8.80E-44"
                     /label="ASC82_RS30910_PKS_AT.1"
                     /locus_tag="ASC82_RS30910"
                     /protein_end="132"
                     /protein_start="0"
                     /score="142.0"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Ethylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="MGADLLDSSQEFGAAMAECETAFAPYVDWSLEAVVRQTPGAPSLE
                     RVDVVQPVTFAVMASLARVWQHYGVTPQAVIGHSQGEIAAAYVAGALTLDDAARVVALR
                     SQSIATHLAGKGGMISLALSESAALERL"
     CDS_motif       complement(1221..1331)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30910_0002"
                     /evalue="3.30E-21"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="ASC82_RS30910"
                     /protein_end="105"
                     /protein_start="68"
                     /score="66.9"
                     /tool="antismash"
                     /translation="GVTPQAVIGHSQGEIAAAYVAGALTLDDAARVVALRS"
     CDS_motif       complement(1338..1406)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30910_0001"
                     /evalue="1.40E-10"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="ASC82_RS30910"
                     /protein_end="66"
                     /protein_start="43"
                     /score="33.1"
                     /tool="antismash"
                     /translation="LERVDVVQPVTFAVMASLARVWQ"
     assembly_gap    1568
                     /estimated_length=1
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1569..3462)
                     /locus_tag="ASC82_RS30915"
                     /old_locus_tag="ASC82_29100"
                     /pseudo=""
     CDS             complement(<1569..3462)
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (4-32). E-value:
                     1.5e-14. Score: 45.2. Matches aSDomain:
                     nrpspksdomains_ASC82_RS30915_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (36-466). E-value:
                     4.8e-175. Score: 574.4. Matches aSDomain:
                     nrpspksdomains_ASC82_RS30915_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060788.1"
                     /locus_tag="ASC82_RS30915"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_29100"
                     /product="type I polyketide synthase"
                     /pseudo=""
                     /sec_met_domain="mod_KS (E-value: 4e-224, bitscore: 732.2,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.7e-92, bitscore:
                     297.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.2e-19, bitscore: 58.9,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANNEDKLRDYLKRVTAELQQNTKRLREIEGRTHEPIAIVGMACR
                     LPGGVASPEDLWQLVAGDGDAISEFPEDRGWDVEGLYDPDPDASGRTYCRSGGFLHDAG
                     EFDADFFGISPREALAMDPQQRLSLTTAWEAIEHAGIDPTTLKGSGLGVFVGGWHTGYT
                     SGQSTALQSPELEGHLVSGAALGFLSGRIAYVLGTDGPALTVDTACSSSLVALHLAVQA
                     LRKGECDMALAGGVTVMPNADLFVQFSRQRGLAADGRSKAFATSADGFGPAEGAGVLLV
                     ERVSDARRNGHRVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRRALADARLAAGDVDV
                     VEAHGTGTRLGDPIEAQALIATYGRERGSEQPLRLGALKSNIGHTQAAAGVAGVIKMVQ
                     AMRHGLLPKTLHVDEPSDQIDWSAGTVELLTEAVDWPEKTDGGLRRAAVSSFGISGTNA
                     HVVIEEAPVASEATEAVESPAGVVPWLVSAKTPAALDAQIGRLSAYAESRTDLDPAAAA
                     RALLDGRTAMEYRAVAVGNGREALRDALRMPEGLVRGTASDLGRVAFVFPGQGTQWAGM
                     GAELLDVSKEFAEAMAECETALSPYVDWSLEAVVRQTPGAPSLERVDVVQPVTFA"
     CDS_motif       complement(1720..1773)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30915_0004"
                     /evalue="2.30E-09"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="ASC82_RS30915"
                     /protein_end="581"
                     /protein_start="563"
                     /score="28.9"
                     /tool="antismash"
                     /translation="FVFPGQGTQWAGMGAELL"
     aSDomain        complement(2065..3354)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS30915_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="4.80E-175"
                     /label="ASC82_RS30915_PKS_KS.1"
                     /locus_tag="ASC82_RS30915"
                     /protein_end="466"
                     /protein_start="36"
                     /score="574.4"
                     /tool="antismash"
                     /translation="IAIVGMACRLPGGVASPEDLWQLVAGDGDAISEFPEDRGWDVEGL
                     YDPDPDASGRTYCRSGGFLHDAGEFDADFFGISPREALAMDPQQRLSLTTAWEAIEHAG
                     IDPTTLKGSGLGVFVGGWHTGYTSGQSTALQSPELEGHLVSGAALGFLSGRIAYVLGTD
                     GPALTVDTACSSSLVALHLAVQALRKGECDMALAGGVTVMPNADLFVQFSRQRGLAADG
                     RSKAFATSADGFGPAEGAGVLLVERVSDARRNGHRVLAVVRGSAVNQDGASNGLTAPNG
                     PSQQRVIRRALADARLAAGDVDVVEAHGTGTRLGDPIEAQALIATYGRERGSEQPLRLG
                     ALKSNIGHTQAAAGVAGVIKMVQAMRHGLLPKTLHVDEPSDQIDWSAGTVELLTEAVDW
                     PEKTDGGLRRAAVSSFGISGTNAHVVIEEAP"
     CDS_motif       complement(2293..2337)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30915_0003"
                     /evalue="1.60E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="ASC82_RS30915"
                     /protein_end="390"
                     /protein_start="375"
                     /score="11.0"
                     /tool="antismash"
                     /translation="GALKSNIGHTQAAAG"
     CDS_motif       complement(2398..2442)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30915_0002"
                     /evalue="8.30E-05"
                     /label="PKSI-KS_m5"
                     /locus_tag="ASC82_RS30915"
                     /protein_end="355"
                     /protein_start="340"
                     /score="14.4"
                     /tool="antismash"
                     /translation="VEAHGTGTRLGDPIE"
     CDS_motif       complement(2818..2865)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS30915_0001"
                     /evalue="2.80E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="ASC82_RS30915"
                     /protein_end="215"
                     /protein_start="199"
                     /score="22.3"
                     /tool="antismash"
                     /translation="GPALTVDTACSSSLVA"
     aSDomain        complement(3367..3450)
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS30915_PKS_Docking_Nterm.
                     1"
                     /evalue="1.50E-14"
                     /label="ASC82_RS30915_PKS_Docking_Nterm.1"
                     /locus_tag="ASC82_RS30915"
                     /protein_end="32"
                     /protein_start="4"
                     /score="45.2"
                     /tool="antismash"
                     /translation="EDKLRDYLKRVTAELQQNTKRLREIEGR"
     gene            complement(3555..>8159)
                     /locus_tag="ASC82_RS04435"
                     /old_locus_tag="ASC82_29105"
     CDS             complement(3555..>8159)
                     /NRPS_PKS="Domain: PKS_AT (0-174). E-value: 3.7e-51. Score:
                     166.2. Matches aSDomain:
                     nrpspksdomains_ASC82_RS04435_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (248-414). E-value: 5.9e-68.
                     Score: 220.4. Matches aSDomain:
                     nrpspksdomains_ASC82_RS04435_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_ER (749-1056). E-value: 3.7e-117.
                     Score: 382.9. Matches aSDomain:
                     nrpspksdomains_ASC82_RS04435_PKS_ER.1"
                     /NRPS_PKS="Domain: PKS_KR (1065-1245). E-value: 2.7e-55.
                     Score: 179.1. Matches aSDomain:
                     nrpspksdomains_ASC82_RS04435_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (1360-1432). E-value: 2.7e-34.
                     Score: 109.3. Matches aSDomain:
                     nrpspksdomains_ASC82_RS04435_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (1467-1531). E-value:
                     3.3e-14. Score: 45.5. Matches aSDomain:
                     nrpspksdomains_ASC82_RS04435_PKS_Docking_Cterm.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="ASC82_RS04435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASC82_29105"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_157863434.1"
                     /sec_met_domain="PKS_AT (E-value: 2.5e-52, bitscore: 166.2,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_N (E-value: 5e-11, bitscore: 30.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.5e-18, bitscore:
                     54.5, seeds: 86, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 4.4e-61, bitscore:
                     194.0, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.3e-17, bitscore:
                     51.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VVALRSQSIATHLAGKGGMISLALSESAALERLVHFDGLSVAAVN
                     GPTATVVSGDPIQIQELANSCETDGIRARIIPVDYASHSPHVEIIEAELAEVLAGLNPQ
                     TPRVPFFSTLEGAWITGPVLDGAYWYRNLRHRVGFAPAVATLAADEGFTHFVEVSAHPV
                     LTMALPETVTGLGTLRREQGGQTRLVTSLAEAWTNGLPVDWTPLLPVATTHADLPTYAF
                     QTERYWPQPDLSATGDITSAGLGAAEHPLLGAAVPLADSDGCLLTGSLSLRTHPWLADH
                     AVSGTVLLPGTAFVELAFRAGDQAGCDLVEELTLDAPLVLPRRGAVRVQLSVGAGDESG
                     RRTFALYAHPEDASGEAVWTRHATGVLAVRTQDATPAADTEAWPPAGAEPVDVDGLYER
                     FAASGYGYGPLFQGVRGVWRRGDEVFADVALPTEVAGAEGARFGLHPALLDAAVQAAGA
                     GGAFGEGTRLPFAWSGLSLYAVGATALRVRLAPVGPDTVSLTAADATGQPVLAADSLTV
                     LPVDPAQLAALADPTPDSLHVLEWTAWDGAAQAALPGTVVLGADADGFAAALRAGGTDV
                     LAFPDLAALAQAVGAGGTPAPTTVLAACPAPGPGGPEHVREALHGALGLMQAWLADERF
                     ADARLVLVTRDAVAARPGDALLSTGQAAVWGLGRSAQTESPGRIVLLDLSGGALGDTLA
                     AALGSDEPQLAVRDGALLVPRLARATASGAAGGLAALPLPAAPALWRLEPGTDGSLESL
                     AAAPGDADSLAPEPLGPGQVRIAVRATGLNFRDVLIALGMYPDPALMGTEGAGVVTATG
                     PGVTHLAPGDRVMGLLSGAYAPVVVADARTVARMPEGWTFAQAASVPVVFLTAVYALRD
                     LADVKPGERLLVHAAAGGVGMAAVQLARHWGVEVHGTASPGKWEALRRLGLDDAHISSS
                     RDLDFEAVFRTTSGGAGMDVVLNSLAREFVDASLRLLGPGGRFVEMGKTDVRDAEKVAA
                     DHPGVGYRAFDLGEAGPERIGEMLAEVLALFESGVLEHLPVAAWDVRRARDAFRHVSQA
                     RHTGKVVLTMPSGLDPEGTVLVTGGTGALGGIVARHVVSEWGVRRLLLVSRRGTDAPGA
                     AELVAGLEALGAEVSVAACDVADRDSLTGLLAGVPAEHPLTAVVHTAGVLSDGTVPSMT
                     TADVEHVLRPKVDAAFLLDELTSASDLAAFVMFSSAAAVFGGAGQGAYAAANAALDALA
                     FRRRAAGLPALSLGWGLWAENSGMTGGLSDTDRSRLARSGATPMDSALTLSLLDAAMRR
                     DDPALVPIALDVTALRAQERDGMLAPLLSGLTRGARAGGAPVNQRRTAAGGPAEGDTDL
                     AGRLAAMTADDRAAHLRDLVRTHVATVLGHGTPSRVDTERAFRDTGFDSLTAVELRNRL
                     NAATGLRLPATLVFDHPTPGELAAHLLDELAAGAGAAWADGSGSASGSGSGSGIEGSAT
                     DRQTAAALAELDRLEGVLASLAPAAGGRPELAARLRALAAALGDDGDDATDLDEASDDD
                     LFSFIDRELGDSDS"
     aSDomain        complement(3564..3755)
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS04435_PKS_Docking_Cterm.
                     1"
                     /evalue="3.30E-14"
                     /label="ASC82_RS04435_PKS_Docking_Cterm.1"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="1531"
                     /protein_start="1467"
                     /score="45.5"
                     /tool="antismash"
                     /translation="ALAELDRLEGVLASLAPAAGGRPELAARLRALAAALGDDGDDATD
                     LDEASDDDLFSFIDRELGD"
     aSDomain        complement(3861..4076)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS04435_ACP.1"
                     /evalue="2.70E-34"
                     /label="ASC82_RS04435_ACP.1"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="1432"
                     /protein_start="1360"
                     /score="109.3"
                     /tool="antismash"
                     /translation="DLVRTHVATVLGHGTPSRVDTERAFRDTGFDSLTAVELRNRLNAA
                     TGLRLPATLVFDHPTPGELAAHLLDEL"
     aSModule        complement(3861..8156)
                     /complete
                     /domains="nrpspksdomains_ASC82_RS04435_PKS_AT.1"
                     /domains="nrpspksdomains_ASC82_RS04435_PKS_DH.1"
                     /domains="nrpspksdomains_ASC82_RS04435_PKS_ER.1"
                     /domains="nrpspksdomains_ASC82_RS04435_PKS_KR.1"
                     /domains="nrpspksdomains_ASC82_RS04435_ACP.1"
                     /locus_tags="ASC82_RS04435"
                     /monomer_pairings="mmal -> Me-redmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(4422..4961)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS04435_PKS_KR.1"
                     /evalue="2.70E-55"
                     /label="ASC82_RS04435_PKS_KR.1"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="1245"
                     /protein_start="1065"
                     /score="179.1"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="GTVLVTGGTGALGGIVARHVVSEWGVRRLLLVSRRGTDAPGAAEL
                     VAGLEALGAEVSVAACDVADRDSLTGLLAGVPAEHPLTAVVHTAGVLSDGTVPSMTTAD
                     VEHVLRPKVDAAFLLDELTSASDLAAFVMFSSAAAVFGGAGQGAYAAANAALDALAFRR
                     RAAGLPALSLGWGLWAE"
     CDS_motif       complement(4470..4577)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0012"
                     /evalue="3.30E-16"
                     /label="PKSI-KR_m4"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="1229"
                     /protein_start="1193"
                     /score="51.8"
                     /tool="antismash"
                     /translation="AAFVMFSSAAAVFGGAGQGAYAAANAALDALAFRRR"
     CDS_motif       complement(4857..4958)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0011"
                     /evalue="3.00E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="1100"
                     /protein_start="1066"
                     /score="19.9"
                     /tool="antismash"
                     /translation="TVLVTGGTGALGGIVARHVVSEWGVRRLLLVSRR"
     CDS_motif       complement(4989..5036)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0010"
                     /evalue="7.00E-06"
                     /label="PKSI-ER_m9"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="1056"
                     /protein_start="1040"
                     /score="18.9"
                     /tool="antismash"
                     /translation="AFRHVSQARHTGKVVL"
     aSDomain        complement(4989..5909)
                     /ASF="ER domain putatively catalyzing 2S-configuration
                     product formation"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS04435_PKS_ER.1"
                     /evalue="3.70E-117"
                     /label="ASC82_RS04435_PKS_ER.1"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="1056"
                     /protein_start="749"
                     /score="382.9"
                     /tool="antismash"
                     /translation="LESLAAAPGDADSLAPEPLGPGQVRIAVRATGLNFRDVLIALGMY
                     PDPALMGTEGAGVVTATGPGVTHLAPGDRVMGLLSGAYAPVVVADARTVARMPEGWTFA
                     QAASVPVVFLTAVYALRDLADVKPGERLLVHAAAGGVGMAAVQLARHWGVEVHGTASPG
                     KWEALRRLGLDDAHISSSRDLDFEAVFRTTSGGAGMDVVLNSLAREFVDASLRLLGPGG
                     RFVEMGKTDVRDAEKVAADHPGVGYRAFDLGEAGPERIGEMLAEVLALFESGVLEHLPV
                     AAWDVRRARDAFRHVSQARHTGKVVL"
     CDS_motif       complement(5292..5327)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0009"
                     /evalue="9.90E-04"
                     /label="PKSI-ER_m7"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="955"
                     /protein_start="943"
                     /score="11.8"
                     /tool="antismash"
                     /translation="GGAGMDVVLNSL"
     CDS_motif       complement(5463..5525)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0008"
                     /evalue="1.10E-08"
                     /label="PKSI-ER_m5"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="898"
                     /protein_start="877"
                     /score="27.4"
                     /tool="antismash"
                     /translation="GERLLVHAAAGGVGMAAVQLA"
     CDS_motif       complement(5550..5594)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0007"
                     /evalue="7.10E-05"
                     /label="PKSI-ER_m4"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="869"
                     /protein_start="854"
                     /score="16.1"
                     /tool="antismash"
                     /translation="AASVPVVFLTAVYAL"
     CDS_motif       complement(5781..5816)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0006"
                     /evalue="2.00E-04"
                     /label="PKSI-ER_m1"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="792"
                     /protein_start="780"
                     /score="14.2"
                     /tool="antismash"
                     /translation="GLNFRDVLIALG"
     aSDomain        complement(6915..7412)
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS04435_PKS_DH.1"
                     /evalue="5.90E-68"
                     /label="ASC82_RS04435_PKS_DH.1"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="414"
                     /protein_start="248"
                     /score="220.4"
                     /tool="antismash"
                     /translation="HPLLGAAVPLADSDGCLLTGSLSLRTHPWLADHAVSGTVLLPGTA
                     FVELAFRAGDQAGCDLVEELTLDAPLVLPRRGAVRVQLSVGAGDESGRRTFALYAHPED
                     ASGEAVWTRHATGVLAVRTQDATPAADTEAWPPAGAEPVDVDGLYERFAASGYGYGPLF
                     QGV"
     CDS_motif       complement(7254..7328)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0005"
                     /evalue="2.00E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="301"
                     /protein_start="276"
                     /score="33.1"
                     /tool="antismash"
                     /translation="WLADHAVSGTVLLPGTAFVELAFRA"
     aSDomain        complement(7635..8156)
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: False, scaffold matched
                     GHGE: False"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC82_RS04435_PKS_AT.1"
                     /evalue="3.70E-51"
                     /label="ASC82_RS04435_PKS_AT.1"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="174"
                     /protein_start="0"
                     /score="166.2"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: (unknown)"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VVALRSQSIATHLAGKGGMISLALSESAALERLVHFDGLSVAAVN
                     GPTATVVSGDPIQIQELANSCETDGIRARIIPVDYASHSPHVEIIEAELAEVLAGLNPQ
                     TPRVPFFSTLEGAWITGPVLDGAYWYRNLRHRVGFAPAVATLAADEGFTHFVEVSAHPV
                     LTMALPETVTG"
     CDS_motif       complement(7665..7694)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0004"
                     /evalue="7.20E-03"
                     /label="PKSI-AT-mM_m9"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="164"
                     /protein_start="154"
                     /score="9.2"
                     /tool="antismash"
                     /translation="FVEVSAHPVL"
     CDS_motif       complement(7740..7787)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0003"
                     /evalue="1.80E-04"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="139"
                     /protein_start="123"
                     /score="13.9"
                     /tool="antismash"
                     /translation="LDGAYWYRNLRHRVGF"
     CDS_motif       complement(7893..7925)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0002"
                     /evalue="2.50E-04"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="88"
                     /protein_start="77"
                     /score="13.7"
                     /tool="antismash"
                     /translation="VDYASHSPHVE"
     CDS_motif       complement(7995..8033)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC82_RS04435_0001"
                     /evalue="9.70E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="ASC82_RS04435"
                     /protein_end="54"
                     /protein_start="41"
                     /score="12.4"
                     /tool="antismash"
                     /translation="AAVNGPTATVVSG"
ORIGIN
        1 ggcgaggcgg cgggcgtgga gtccggtggt gcgtatggcg atttggtcct cgccggtgct
       61 gccggcgagg acggtcacga ggtggccgag ggtcgacgtg tcgagcttgg tggggaggtc
      121 gataagaccg gcccagcgtt cggggtgttc gagggcgacg acgcggccga ggccccagag
      181 catggcctgg tcggggtcgg cgggcgtgtc gtggcggccg acggaaacgg cgccccgggt
      241 gacgcaccac agaggcgcct cgatcccggc gtcgccgagc gcctgcaccc aggcgacgag
      301 gggcgtgagt ccgtcgagga gggagatcac gccggtgaac ccgtccgtca gccccgcgag
      361 ccgggcggcg agcgcctcac ggtcgtcgtc cgcacccgtc tccaccacct ccacctcggc
      421 tccggcggcc ttcagcgcgc cgagcagccc ggcgtcgggc tcgccaccgg cggcgaccag
      481 ccaccggccg gccaggtccg cctccgcggc ggtcgccagg ggcttccagt cgacgcggta
      541 gcgccagtcg tcggccgtac tggtgggcgc ggaggcctgg agccagtagc gctcggtctg
      601 gaaggcgtag gtggggaggt cggcgtgggt ggccgtggca gggaggtggg aagtccagtc
      661 gacggggagg ccgttggccc acgcctcggc gagggaggtg acgagccggt cctgtccgcc
      721 ctgctcgcgg cggagggtgc cgagtccggt gacggtctcg gggagggcca tggtgaggac
      781 ggggtgggcg ctgacctcga cgaagtgcgt gaacccctcg tgggtggcca gggtctcgac
      841 ggcgggggcg aagccgacgc ggtggcggag gttgcggtac caatacgcgg cgtcgagggc
      901 aggttccgtg atccagccgc cttcgagggt ggagaagaac ggcacctgcg gcacctgcgg
      961 ggtcagcccg gcgaggacct cggcgagctc cttctcgatg atctcgacct gagcgctgtg
     1021 ggaggcgtag tcgacgggga tgatccgcgc gcggatcccg tcggtctcac aggagttggc
     1081 gagttcctgg atctggatcg ggtcgcccga gannnnnnnn nnnnnnnnnn nnngtccacg
     1141 agtcgttcga gggcggcact ctcgctgagg gcgagggaga tcatgccgcc cttgccggcg
     1201 aggtgggtgg cgatggactg gctgcgcagg gccacgaccc gagcggcgtc gtccagagtc
     1261 agcgccccgg cgacgtacgc ggcggcgatc tcgccctggg agtggccgat gacggcctgc
     1321 ggcgtcacgc cgtagtgctg ccacacgcgg gccagggacg ccatgacggc gaaggtgacg
     1381 ggctggacga cgtcgacccg ctccagtgag ggcgcacccg gcgtctgccg gacgacggcc
     1441 tcaagggacc agtcgacgta cggggcgaac gcggtctcgc actcggccat cgcggcgccg
     1501 aactcctgtg atgagtcgag gaggtcggcg cccatcccgg cccactgcgt gccctggccg
     1561 gggaacancg gcgaaggtga cgggctggac gacgtcgacc cgctccagtg agggcgcacc
     1621 cggcgtctgc cggacgacgg cctcaaggga ccagtcgacg tacggggaga gtgcggtctc
     1681 gcactcggcc atggcttccg cgaactcctt cgatacgtcg aggagttcgg cgcccatccc
     1741 ggcccactgc gtgccctggc cggggaacac gaacgccacg cgaccgaggt cggacgccgt
     1801 gccgcgtacc agcccctccg gcatccgcag ggcgtcccgc aaagcctccc ggccgttgcc
     1861 gaccgcgacc gcgcggtact ccatcgccgt gcgcccgtcg agcagtgcac gggccgccgc
     1921 ggccgggtcc aggtccgtac ggctctccgc gtacgcggag agccgtccga tctgggcgtc
     1981 gagcgcggcg ggggtcttcg cggacacgag ccacggcacg acaccggccg gggactcgac
     2041 ggcctccgtc gcctcggagg cgaccggggc ctcttcgatc accacgtggg cgttcgtgcc
     2101 gctgatgccg aaggaggaga ctgccgcccg gcgcagtccg ccgtcggtct tctccggcca
     2161 gtccacggcc tccgtgagga gctcgaccgt gcccgccgac cagtcgatct ggtccgaggg
     2221 ctcgtccacg tggagcgtct tcggaagcag cccgtggcgc atcgcctgga ccatcttgat
     2281 cacaccggcg acaccggccg cggcctgcgt gtgcccgatg ttcgacttca acgcgcccag
     2341 ccgcagcggc tgttcgctgc cgcgctcccg cccgtacgtg gcgatgagcg cctgtgcctc
     2401 gatcgggtcg ccgaggcgcg tacccgtgcc gtgcgcctcg acgacgtcca cgtcgcccgc
     2461 cgcgagccgg gcgtcggcca gggcgcgccg gatcacgcgc tgctgcgacg ggccgttcgg
     2521 ggccgtgagg ccgttgctcg cgccgtcctg gttgacggcg ctgccgcgga cgaccgcgag
     2581 gacgcggtgg ccgttgcggc gtgcgtccga cacgcgctcc accaggagga cgccggcgcc
     2641 ctccgccggg ccgaagccgt ccgccgacgt ggcgaacgcc ttcgaccggc cgtccgcggc
     2701 caggccgcgc tgccggctga actggacgaa gaggtcggcg ttgggcatga ccgtgacgcc
     2761 gccggcgagg gccatgtcgc actcgccctt gcggagggcc tgcacggcga ggtgcagggc
     2821 gacgagcgac gacgagcacg ccgtgtcgac cgtcagggcc ggtccgtccg taccgaggac
     2881 gtacgcgatg cggccggaca ggaagcccag cgccgcaccg ctgaccaggt ggccctccag
     2941 ctccggcgac tgcagggccg tgctctggcc cgaggtgtag ccggtgtgcc agccgccgac
     3001 gaagacgccg agaccgctgc ccttcagggt cgtcgggtcg atgccggcgt gctcgatcgc
     3061 ctcccacgcg gtggtgaggg acagtcgctg ctgcgggtcc atggcgaggg cctcgcgggg
     3121 cgagatcccg aagaagtcgg cgtcgaactc acccgcgtcg tgcaggaatc cgccggaccg
     3181 gcaatacgtc cgtccggagg cgtccgggtc cgggtcgtac agcccctcga cgtcccagcc
     3241 gcggtcctca gggaactccg agatcgcgtc cccgtccccg gccaccagct gccacaggtc
     3301 ctccggcgag gcgacaccgc ccggcaggcg gcacgccatg cccacgatcg cgatcggctc
     3361 gtgcgtgcgt ccctcgatct cgcgcagacg cttggtgttc tgctgcagct cggcggtgac
     3421 gcgcttgaga tagtcgcgga gcttgtcttc gttgttcgcc atggctcccg ttctcgcctg
     3481 tccgttccgt gtctgagggg ctggtggtgc ctgtggtgcc tgtgggctgg tgtgccgggg
     3541 gggtgtcggt caggtcagga gtcggagtcg cccagctccc tgtcgatgaa ggagaagagg
     3601 tcgtcgtcgg acgcctcgtc caggtcggtg gcgtcgtcgc cgtcgtcccc cagggccgcg
     3661 gccagcgccc tgagccgggc ggcgagctcc ggacggccgc cggcggcggg cgcgagggag
     3721 gcgaggaccc cctccagccg gtcgagttcg gcgagggccg ccgcggtctg ccggtccgtg
     3781 gcggagccct cgatgccgga tccggatccg gatccggacg cggaccccga tccgtccgcc
     3841 caggccgcgc ccgcgcccgc ggcgagttcg tcgagcaggt gcgcggcgag ctctccgggt
     3901 gtcgggtggt cgaagacgag cgtggccggg agccgcagtc cggtggccgc gttgaggcgg
     3961 ttgcggagtt cgacggcggt cagggagtcg aagccggtgt cgcggaaggc gcgctcggtg
     4021 tcgacccggc tcggggtgcc gtgtccgagg acggtcgcca cgtgcgtacg gacgaggtcc
     4081 cgcaggtgtg cggcccggtc gtccgccgtc atcgcggcga gccgcccggc gaggtccgtg
     4141 tccccctcgg cggggccgcc ggcggcggtc ctgcgctggt tgaccggcgc gccgccggcc
     4201 cgcgccccgc gggtgagccc gctgagcagc ggcgccagca tgccgtcgcg ctcctgggcg
     4261 cggagcgcgg tgacgtccag ggcgatcggg acgagcgccg ggtcgtcgcg gcgcatcgcc
     4321 gcgtccagga gggacagggt cagcgcgctg tccatgggcg tcgccccgga acgggcgagc
     4381 cgcgagcggt cggtgtcgct caggccgccg gtcatgccgc tgttctcggc ccagaggccc
     4441 cagccgaggg agagggccgg gagcccggcc gcccggcggc ggaaggcgag ggcgtcgagg
     4501 gcggcgttgg ccgccgcgta cgctccctgg cccgctccgc cgaagacggc ggcggcggag
     4561 gagaacatga cgaacgccgc caggtcggag gcggaggtca gctcgtcgag gaggaacgcc
     4621 gcgtcgacct tgggacgcag gacgtgctcc acgtccgccg tcgtcatcga cgggaccgtt
     4681 ccgtccgaca ggacgcccgc cgtgtggacg accgcggtca gcgggtgctc ggccggaacc
     4741 ccggccagca gcccggtgag cgagtcccgg tccgccacgt cacacgcggc cacggacacc
     4801 tccgcaccga gagcctcaag acctgccacc agctccgccg cgcccggcgc gtccgtaccg
     4861 cgacggctca cgagcagcag gcgccgcacg ccccactcgc tcaccacgtg ccgggccacg
     4921 atgccgccca gcgcgccggt gccgccggtc accaggaccg tcccctccgg gtcgagaccc
     4981 gacggcatgg tcaggacgac cttgcccgtg tgacgcgcct ggctgacgtg ccggaaggcg
     5041 tcgcgggcgc ggcgcacgtc ccacgcggcc acggggaggt gctccagcac gccgctctcg
     5101 aagagggcga gtacctcggc gagcatctcg ccgatccgct ccggcccggc ctcgccgagg
     5161 tcgaacgcgc ggtagccgac gccggggtgg tcggcggcga ccttctccgc gtcgcgcacg
     5221 tcggtcttgc ccatctccac gaaacggccg ccgggaccga gcaggcgcag cgacgcgtcg
     5281 acgaactcgc gggcgagcga gttcaggacg acgtccatgc ccgccccgcc cgaggtcgtg
     5341 cggaacacgg cctcgaagtc gaggtcgcgg gaggagctga tgtgcgcgtc gtccagaccg
     5401 aggcggcgca gggcctccca cttgcccggg ctcgccgtgc cgtggacctc cacgccccag
     5461 tggcgggcga gctgcacggc ggccatgccc acgccgccgg cggcggcgtg gaccaggagg
     5521 cgctcgccgg gcttgacgtc cgccaggtcg cgcagggcgt agacggccgt caggaacacc
     5581 accggcacgg aggcggcctg ggcgaacgtc cacccctcgg gcatccgcgc cacggtccgc
     5641 gcgtcggcga cgacgaccgg ggcgtacgcg ccggagagca ggcccatgac gcggtcgccg
     5701 ggggcgaggt gcgtgacgcc ggggccggtg gcggtgacga cgccggcgcc ctcggtgccc
     5761 atcagcgccg gatcggggta catgccgagg gcgatcagga cgtcacggaa gttgagaccc
     5821 gtggcccgta cggcgatgcg gacctggccg ggaccgagcg gttccggtgc gagggagtcg
     5881 gcgtcgccgg gcgcggccgc gaggctctcc aggctgccgt ccgtaccggg ctccaggcgc
     5941 cagagggccg gagcggcggg aagcggcaga gcggcgaggc cgccggccgc gccggacgcg
     6001 gtggcccgcg ccagccgggg gacgaggagc gcgccgtcgc gcacggcgag ctgcggctcg
     6061 tccgaaccga gggcggccgc cagggtgtcg ccgagcgccc caccggagag gtcgagcagg
     6121 acgatccggc ccgggctctc cgtctgcgcg gaccggccca gaccccacac ggcggcctga
     6181 ccggtcgaca gcagcgcgtc gcccggacgg gcggcgaccg cgtcacgcgt cacgagcacc
     6241 agacgcgcgt cggcgaaccg ctcgtcggcg agccaggcct gcatcagccc gagtgccccg
     6301 tgcagggctt cgcggacgtg ctccggccca ccggggccgg gagcggggca ggccgccagg
     6361 acggtcgtcg gggccggggt cccgcccgcg ccgacggcct gcgccagggc ggcgaggtcg
     6421 gggaaggcga ggacgtcggt gccgccggcg cgcagcgccg cggcgaagcc gtcggcgtcg
     6481 gcgcccagca cgaccgtgcc gggcagcgcc gcctgcgcgg caccgtccca ggcggtccac
     6541 tccaggacgt gcagcgagtc cggcgtcggg tcggcgagcg ccgccagttg cgcggggtcg
     6601 acgggaagca ccgtgaggga gtccgcggcg agtaccggct gcccggtggc gtcggcggcg
     6661 gtcagggaca cggtgtccgg gccgaccggg gccagccgca cccgcagggc ggtggcgccg
     6721 accgcgtaca gcgagagccc gctccaggcg aacggcagac gcgtgccctc gccgaacgcc
     6781 ccgcccgcgc ccgccgcctg cacggcggcg tcgagcagcg ccgggtggag accgaaccgc
     6841 gcgccctcgg caccggcgac ctcggtcggc agggccacgt cggcgaacac ctcgtcgccg
     6901 cgccgccaga caccacggac gccctggaag agggggccgt agccgtagcc gctcgccgcg
     6961 aaacgctcgt acagaccgtc cacgtccacc ggctcggcgc ccgccggggg ccaggcctcc
     7021 gtgtcggcgg cgggcgtggc gtcctgcgtg cggacggcca gcacaccggt ggcgtggcgc
     7081 gtccacaccg cctcgcccga cgcgtcctcg gggtgagcgt agagggcgaa ggtacggcga
     7141 ccggactcgt cgcccgcacc gacggacagc tgcacgcgga ccgcgccgcg gcggggcagg
     7201 acgagcggcg cgtcgagggt gagctcctcg accagatcgc agccggcctg gtccccggcc
     7261 cggaacgcca gctccacgaa ggccgtcccc ggcagcagta cggtgccgga caccgcgtgg
     7321 tccgccagcc aggggtgcgt acggagggac aggctgcccg tgagcaggca gccgtcggag
     7381 tccgcgagcg gaacggccgc accgagcagc gggtgctcgg ccgccccgag accggcggag
     7441 gtgatgtcac cggtggcgga gaggtcgggc tgcggccagt agcgctcggt ctggaaggcg
     7501 tacgtgggga ggtcggcgtg ggtggtcgcg acgggcagga gcggcgtcca gtccacgggg
     7561 aggccgttgg tccacgcctc ggcgagggag gtgacgaggc gggtctgtcc gccctgctcg
     7621 cggcggaggg tgccgaggcc ggtgacggtc tcggggagtg ccatggtgag gacggggtgg
     7681 gcgctgacct cgacgaagtg cgtgaagcct tcgtcggcgg cgagcgtcgc gaccgccggc
     7741 gcgaagccga cgcggtggcg gaggttgcgg taccagtagg cgccgtccag gacggggccg
     7801 gtgatccatg cgccttcgag ggtggagaag aatggcacgc ggggggtctg cgggttcagc
     7861 ccggcgagga cttcggcgag ctcggcctcg atgatctcga cgtgggggct gtgggaggcg
     7921 tagtcgacgg ggatgatccg cgcgcggatc ccgtcggtct cacaggagtt ggcgagttcc
     7981 tggatctgga tcgggtcgcc cgagaccacg gtcgcggtcg gcccgttgac cgccgccacg
     8041 gacaacccgt cgaagtgcac gagtcgttcg agggcggcac tctcgctgag ggcgagggag
     8101 atcatgccgc ccttgccggc gaggtgggtg gcgatggact ggctgcgcag ggccacgac
//
