LOCUS       NZ_LMEQ01000032        11443 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root1310 contig_6, whole genome shotgun sequence.
ACCESSION   NZ_LMEQ01000032
VERSION     NZ_LMEQ01000032.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root1310
  ORGANISM  Streptomyces sp. Root1310
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 711102)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 711102)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140604
            Assembly Name                     :: Root1310
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426425.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:14:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,470
            CDSs (total)                      :: 8,385
            Genes (coding)                    :: 8,123
            CDSs (with protein)               :: 8,123
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 98 of 262
            Pseudo Genes (incomplete)         :: 219 of 262
            Pseudo Genes (internal stop)      :: 20 of 262
            Pseudo Genes (multiple problems)  :: 64 of 262
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:32:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 301400
            Orig. end                         :: 312843
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEQ01000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11443
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11443
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11443
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6443](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6443)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(561..575)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.73"
                     /tool="antismash"
     gene            575..1306
                     /gene="hemQ"
                     /locus_tag="ASD48_RS34560"
                     /old_locus_tag="ASD48_24975"
     CDS             575..1306
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="ASD48_RS34560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_24975"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_057605812.1"
                     /transl_table=11
                     /translation="MSDDAPTTESGRVPNKGKLAKDLNEVIRYTLWSVFRLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADVMIWWHAETADQLQEAYNLFRRTK
                     LGRALDPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFYTGRRKDLTELVAGLA"
     gene            complement(1307..2917)
                     /locus_tag="ASD48_RS34565"
                     /old_locus_tag="ASD48_24980"
     CDS             complement(1307..2917)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (109-482). E-value:
                     7.1e-23. Score: 73.4. Matches aSDomain:
                     nrpspksdomains_ASD48_RS34565_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125237.1"
                     /locus_tag="ASD48_RS34565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_24980"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_057605813.1"
                     /sec_met_domain="PF00561 (E-value: 5.8e-27, bitscore: 90.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALHSAAGALLLTALSAVPAGGAPNTGGPSGSPGVALTAARA
                     AAKGVDFGKCSAAQDLPGSMQCGTVSVPLDYAAPNGKQIELTVSRVRATHRDPHNSKHR
                     VPRQGALVFNPGGPGGSGLYFPLIGLLPEWKRIGAAYDLVGYEPRGVGRSAPLSCQDPK
                     RFFKGPAPAPDHPSESYKRERIAQAKAYAQGCAKRSGSALRHYNSLNNARDLDVLRAAL
                     GEERLTFMGASYGTYFGAVYATLFPSHVRRMVFDAAVNPDPAQIWYRNNLDQSAAFESR
                     WTDFREWIARHDDVYGLGATAEAVLSSYERARARLAAEPAGGKVGPGQLQGAFLQAGYY
                     DDYWPARAQALSEYLKGDPKQLIAQAGPVREAAAEAENANAVYTAVECNDASWPTDWRV
                     WDRDNTRLARVAPFETWDNVWANLPCAYWPAPRQQPVDVRTGPGELPPTLILAAERDAA
                     APYAGALEMHRRLTGSVLVTERDAGTHGIAGGPNRCVNGHLEAYLLEGRLPVRRAACAP
                     RREPEPAVAPAERAARPGR"
     aSDomain        complement(1472..2590)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS34565_Abhydrolase_1.1"
                     /evalue="7.10E-23"
                     /label="ASD48_RS34565_Abhydrolase_1.1"
                     /locus_tag="ASD48_RS34565"
                     /protein_end="482"
                     /protein_start="109"
                     /score="73.4"
                     /tool="antismash"
                     /translation="ALVFNPGGPGGSGLYFPLIGLLPEWKRIGAAYDLVGYEPRGVGRS
                     APLSCQDPKRFFKGPAPAPDHPSESYKRERIAQAKAYAQGCAKRSGSALRHYNSLNNAR
                     DLDVLRAALGEERLTFMGASYGTYFGAVYATLFPSHVRRMVFDAAVNPDPAQIWYRNNL
                     DQSAAFESRWTDFREWIARHDDVYGLGATAEAVLSSYERARARLAAEPAGGKVGPGQLQ
                     GAFLQAGYYDDYWPARAQALSEYLKGDPKQLIAQAGPVREAAAEAENANAVYTAVECND
                     ASWPTDWRVWDRDNTRLARVAPFETWDNVWANLPCAYWPAPRQQPVDVRTGPGELPPTL
                     ILAAERDAAAPYAGALEMHRRLTGSVLVTERDA"
     gene            complement(3049..3825)
                     /locus_tag="ASD48_RS34570"
                     /old_locus_tag="ASD48_24985"
     CDS             complement(3049..3825)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125238.1"
                     /locus_tag="ASD48_RS34570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_24985"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_057605814.1"
                     /transl_table=11
                     /translation="MVWVLLLLLAWAVAGAACTRLCLAAVRAAAVDENAGGEHDLTLYE
                     AAFLSGGPGRVADLTMVSMARQRRLVLARTGWATVVDPRGRDEMERSVIGAIGPEGQSR
                     IAPARAAAAAADAVGGLADRLVRAGLAVPDGARTTVAAGVRQVRVAAVAVLALGATALL
                     VPATPDIPRNLVALWFALPLALTLSCLAIARVEVHPYSRWASPAGQRLLGALARHAGGT
                     GDDHTYLTSVAVRGIRAVGEPDLRAAFAHREQYGRD"
     gene            complement(3951..4727)
                     /locus_tag="ASD48_RS34575"
                     /old_locus_tag="ASD48_24990"
     CDS             complement(3951..4727)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125239.1"
                     /locus_tag="ASD48_RS34575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_24990"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_057605815.1"
                     /transl_table=11
                     /translation="MRPRPPVQGRGIFSGTGLIITGLAATLAALVFPIWSYADRSGTGV
                     DVLNAQTVSTQYGPLSALDREFVTKVRLAGLWELPAGQQAQTKGTTEAVRTAGRHLVEG
                     HTFLDERVRTVASRLGLALPNEPNEQQQGWLGELDAAQGEQYDWKFANILRLAHGRVFS
                     VVAQVRASTRNSLVRELADDANTTVLDHIKILEATGFVDFDALARDMAAGSTPPITRSP
                     GPPGPTADPGSAVPVTPSASPTYTLPPAAASPPPGD"
     misc_feature    complement(4826..4837)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.87"
                     /tool="antismash"
     gene            complement(5001..6443)
                     /locus_tag="ASD48_RS34580"
                     /old_locus_tag="ASD48_24995"
     CDS             complement(5001..6443)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125240.1"
                     /locus_tag="ASD48_RS34580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_24995"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_057605816.1"
                     /sec_met_domain="DUF692 (E-value: 4.2e-97, bitscore: 319.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIADAVERMPGIDWVEAVAENLCPGHLPDSLLR
                     LRERGVTVIPHGVSLGLGDAERPDAGRLAALAERAEALGSPLVTEHIAFVRAGGPLTAS
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQEALPVPLAVENIAALIAWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTDLASRVSPPGVLLERDENFPEPAELERELGTIRAAVEAGEREGAPA
                     RASSATGEHAPTPGTGVGTRTATGIGTRTMPRTATAMGIGTRTASGDGGAPPISEPGPA
                     ALDEATRQRLGLAQAALLSALVAGTPVPEGFDRVRLGVQARALAAKRADVVAKVAPELP
                     LILGSGYRNAFIAYAQSHPMHGGYRQDALDFAEQTLLGGRLTEGRARRELRRWWLDRSG
                     PAPRPKSRLGRAGRVGRTWIRR"
     gene            complement(6680..7402)
                     /locus_tag="ASD48_RS34585"
                     /old_locus_tag="ASD48_25000"
     CDS             complement(6680..7402)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125241.1"
                     /locus_tag="ASD48_RS34585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_25000"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_057605817.1"
                     /transl_table=11
                     /translation="MTSDPAVPGDSPFRSERSTRDEAPQFVLPLVVRIERADPPARTDA
                     LETAARAVLTLLADERALGEGEWAADVRDWQDARIRKVVRRARGAEWRRAEALPGITVM
                     GKAAEVRVFPPVPLDGWPKDLAKLQVSGTDLEDPEPPVDIDATAPVIWQNPEVGMSAGK
                     AMAQAGHGAQLAWWELSDAERTAWREAGFPLAVRTADPRRWRELTESGLPLVRDAGFTE
                     IAPGSCTVVADHPALRAR"
     gene            7460..8320
                     /locus_tag="ASD48_RS34590"
                     /old_locus_tag="ASD48_25005"
     CDS             7460..8320
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125242.1"
                     /locus_tag="ASD48_RS34590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_25005"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_057605818.1"
                     /transl_table=11
                     /translation="MPRELRLEAVGRRYGMRGPWVFRDVHLNPASATLTRVDGPNGTGK
                     STLLRLLARIDAPTEGKVTGAPRTAYVPERFPAALPFTALGYLTHLGAVHGLSRAAAER
                     AAGDWLDRFGATAHAHTPMSRLSKGSSQKVAVAQALLAEPELPILDEAWTGLDTDARAE
                     LERAVAERTAAGSAVVFVDHDPRRLAGRADAEYTVRDGTLHRRAEAEPTAAQDGPHTTI
                     EVHGPTAPLPPEALRLATTTEETADRTHRLTVPASHSDVLLRALLTARPPWHVVSVRPD
                     GSRHR"
     gene            8317..8988
                     /locus_tag="ASD48_RS34595"
                     /old_locus_tag="ASD48_25010"
     CDS             8317..8988
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="ASD48_RS34595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_25010"
                     /product="hypothetical protein"
                     /protein_id="WP_057605819.1"
                     /transl_table=11
                     /translation="MTALLRYQTALLLRSQRWLPPFLLYAVFLGVGVQGGQPLLDSLGY
                     AAAFLLPVAAWLVRICVTNEPPAARTVVAAAVGPGRAHLACVLVALTTAVVFGTAATTT
                     VTLISDPVGNDHDKRVPVLEAAGAGLLAVLTCALLGAAVGALTAWPVLRSPGRAVPALL
                     LAALLALVVTGSPAQAAVSGLVSGSRSATVHVPLLPLAAAALLSAVATAVACALTSRRA
                     P"
     gene            complement(9440..9628)
                     /locus_tag="ASD48_RS34600"
                     /old_locus_tag="ASD48_25015"
     CDS             complement(9440..9628)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125244.1"
                     /locus_tag="ASD48_RS34600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_25015"
                     /product="hypothetical protein"
                     /protein_id="WP_057605820.1"
                     /transl_table=11
                     /translation="MSDDSDREYCLIDATTPPRAEGPPYAECVLCRKPTEYPESYKGIT
                     LCPVCEWQEAQRTACSG"
     gene            complement(9739..10593)
                     /locus_tag="ASD48_RS34605"
                     /old_locus_tag="ASD48_25020"
     CDS             complement(9739..10593)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657146.1"
                     /locus_tag="ASD48_RS34605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_25020"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_057606101.1"
                     /transl_table=11
                     /translation="MILPVRRLAALCALGAALAPLSACGSPHAPRALRPAAPAHTSLAP
                     ARPPTLAPGPAGLTPVFSNAPRTADKRVALTFDADMTADQGARAAAGEHFDNPGLIAAL
                     RALKVPATVFMTGRWAEEYPDQARSLGHDPGFEVANHSYSHYAFTDDCYGLPTVPEDRM
                     RADVERAFTAFRRAGVPDAMPYFRFPGGCYDRRALRAVSGMGVTAVQWDVVSGDAFATD
                     ADAVARQVLEGVRPGSVVVMHCTRSAAPTTEQAVRTIVPELRRKGYRFVKVSELIGAAS
                     GRR"
     misc_feature    complement(10582..10597)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.41"
                     /tool="antismash"
     misc_feature    complement(11271..11285)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 cactgcgcac ggtggaatac gcctccatgg ccctggtcac cctcgcctac cggcgcgccg
       61 acaccgccga cgccctgccc gacggcagcg gtttcctcgt cccgccggtc gacggacgca
      121 ccatcaaggc gtccaccttc tcctcccgga aatggggctg gatcgccgac gaggacccgg
      181 acacggtcgt gctgcgcacc tccgtggggc gctacggaga gacggagatc ctgggccgcg
      241 aggacgccgg cctcgtcgac gtctcccggc acgacctgcg cgcggccacc ggcctggacg
      301 ccacgcccct cgaaacccgc gtcacccgct ggaccgacgg actgccgcag tacccggtcg
      361 gccaccacgc gcgcgtggcc cgcatccgcg agcacgtcgc cgaactcccc ggcctcgcgg
      421 tctgcggcgc cccctacgac ggcgtgggca tcccggcctg catcgcgagc gcgtacgcgg
      481 cggtcgacca gctcggcgga gacctgagcg ccgcacggga gctcgcggcc aacccggtgc
      541 agagtctgca cggcggagcg ggagaatgag agccatgagt gacgacgccc ccaccaccga
      601 gtccggcagg gtcccgaaca agggcaagct ggccaaggac ctcaacgagg tcatccgcta
      661 cacgctgtgg tccgtcttca ggctgaagga cgtgctgccc gaggaccgcg ccggttacgc
      721 cgacgaggtc caggagctgt tcgaccagct cgccgccaag gacgtgacga tccgcggcac
      781 ctatgacgtg tccggcctgc gcgccgacgc cgacgtcatg atctggtggc acgccgagac
      841 cgccgaccag ctccaggagg cgtacaacct cttccgccgt acgaagctgg gccgcgctct
      901 ggacccggtc tggtcgaaca tggcgctgca ccgccccgcc gagttcaacc gctcgcacat
      961 cccggcgttc ctcgccgacg agacgccccg cgactacgtg agcgtctacc ccttcgtgcg
     1021 ctcctacgac tggtacctgc tgcccgacga ggaccgccgc cgcatgctcg ccgaccacgg
     1081 caagatggcc cgcggctacc cggacgtccg cgccaacacc gtcgcctcgt tctcgctggg
     1141 cgactacgag tggatcctgg ccttcgaggc cgacgagctg taccgcatcg tcgacctcat
     1201 gcgccacctg cgcgcctcgg aggcccgcat gcacgtccgc gaggaggtcc cgttctacac
     1261 gggccgtcgc aaggacctca cggagctggt cgcgggcctc gcctgatcag cgccccggcc
     1321 gggcggcgcg ttccgcggga gcgaccgccg gctctggttc ccggcgcggt gcgcaggccg
     1381 cgcgccgcac cgggagcctg ccctccagca ggtacgcctc caggtgaccg ttgacgcacc
     1441 ggttcgggcc gcccgcgatg ccgtgcgtgc ccgcgtcccg ctcggtcacc agcaccgagc
     1501 ccgtcagccg ccggtgcatc tccagcgccc ccgcgtaggg cgccgctgcg tcccgctcgg
     1561 cggccaggat cagcgtcggc ggcagctcgc ccggaccggt ccgcacgtcg accggctgct
     1621 gccggggcgc cggccagtac gcgcacggca ggttcgccca cacgttgtcc cacgtctcga
     1681 acggcgcgac gcgcgccagc cgcgtgttgt cgcggtccca gaccctccag tccgtcggcc
     1741 aggacgcgtc gttgcactcc acggccgtgt acacggcatt ggcgttctcc gcctccgcgg
     1801 cggcctcccg caccggcccg gcctgcgcga tcagctgctt cggatcaccc ttcagatact
     1861 ccgacagcgc ctgcgcccgg gcgggccagt agtcgtcgta gtacccggcc tggaggaacg
     1921 ctccctggag ctgtcccggc cccaccttcc cgccggccgg ctccgcggcc agccgggcgc
     1981 gcgcccgctc gtagctggac agcaccgcct ccgccgtcgc gcccaggccg tacacgtcgt
     2041 cgtgccgcgc gatccactcc cggaagtccg tccagcggct ctcgaacgcc gccgactggt
     2101 cgaggttgtt gcggtaccag atctgcgccg ggtccgggtt cacggccgcg tcgaacacca
     2161 tccgccgtac gtgggagggg aacagcgtcg cgtagaccgc accgaagtag gtgccgtacg
     2221 acgcgcccat gaacgtcagc cgctcctcgc cgagcgcggc gcgcagcacg tcgaggtcgc
     2281 gggcgttgtt gagcgagttg tagtgccgca gggcgctgcc cgaccgcttc gcacagccct
     2341 gtgcgtacgc cttggcctgc gcgatgcgtt cccgcttgta cgactccgag gggtggtccg
     2401 gggccggggc ggggcccttg aagaagcgct tgggatcctg acaggacagg ggcgccgaac
     2461 ggcccacccc gcgcggctcg tagccgacga ggtcgtaggc ggccccgatg cgcttccact
     2521 ccggcagcag cccgatcagc gggaagtaca ggcccgagcc gccgggaccg cccgggttga
     2581 agaccagcgc gccctgccgg ggcacccggt gcttgctgtt gtgcgggtcc cggtgggtgg
     2641 cccgcacccg gctgacggtg agctcgatct gcttgccgtt cggggccgcg tagtcgagcg
     2701 ggacggagac cgtgccgcac tgcatgctgc cgggcaggtc ctgcgccgcg gagcacttcc
     2761 cgaagtcgac gcccttcgcc gcggcccgcg cggcggtcag cgcgacaccg ggcgagccgg
     2821 acggaccgcc ggtgttcggg gcgccgcccg ccgggacggc ggagagggcg gtcagcagga
     2881 gcgctcctgc ggccgagtgg agggcggcgg ctctcatcgg gtgtcccttc ggtgcacggc
     2941 ggcgacaaaa gggatggttg gttcgaccgt gggggaaggc aagcaccatc gacgggtgtc
     3001 ggcgccaatg ccccttgtgc gccccccggt ggtcgagcgc gccccgcctc agtcccgccc
     3061 gtactgctca cgatgggcga aggccgcccg caggtccggc tcgcccaccg cgcggatccc
     3121 gcgtacggcg acggaggtca gataggtatg gtcgtccccc gtgccgccgg cgtgccgcgc
     3181 cagcgcgccc agcagccgct gcccggccgg agaggcccag cgcgagtagg gatgcacctc
     3241 cacccgcgct atggcgaggc agctcagcgt gagcgccagg ggcagcgcga accagagcgc
     3301 gacgaggttg cggggtatgt ccggggtggc ggggaccagc agggcggtgg cgcccagcgc
     3361 gaggacggcc acggcggcga cccgtacctg ccgcaccccg gccgcgaccg tcgtgcgggc
     3421 cccgtcgggc accgcgagac cggcgcggac caaccggtcg gcgaggccgc ccaccgcgtc
     3481 cgcggcggcc gcagccgccc gcgccggggc gatacgggac tgcccctcgg gcccgatggc
     3541 ccctatgacc gaccgctcca tctcgtcccg cccgcgcggg tccaccacgg tcgcccagcc
     3601 cgtgcgggcg agcaccagcc gccgctgccg cgccatcgag accatggtca ggtcggcgac
     3661 cctgcccgga ccaccggaca ggaaggccgc ctcgtagagc gtcagatcgt gttccccgcc
     3721 ggcgttctcg tcgacggccg ccgcccgtac ggcggccagg cacagccgtg tgcacgccgc
     3781 acccgcgacg gcccatgcca gcagcaggag aagcacccag accatgccgc gtttctatgc
     3841 catgcgcacc gagaaacacc atgccttgtt caccatacgg accgagcgtt gtgggtatgt
     3901 gacgttccgc ttgccccgtc cggccctagg aaacggtctc gcaaccggtg tcagtcgccg
     3961 ggcggcgggc tggccgcggc cggcgggagc gtgtaggtgg gggacgccga cggggtcacc
     4021 ggcacggccg acccggggtc ggccgtcggg cccggcgggc cgggggagcg ggtgatcggc
     4081 ggcgtgctgc cggcggccat gtcccgggcg agggcgtcga agtcgacgaa cccggtggcc
     4141 tccaggatct tgatgtggtc cagcacggtg gtgttggcgt cgtcggcgag ctcacgcacc
     4201 agcgagttgc gggtactggc ccgcacctgg gcgacgacgg agaaaacccg tccgtgcgcg
     4261 agacgcagga tgttggcgaa cttccagtcg tactgctcgc cctgcgccgc gtcgagctcg
     4321 cccagccagc cctgctgctg ctcgttgggc tcgttcggca gcgccaggcc cagccgggag
     4381 gcgacggtac gcacgcgctc gtccaggaag gtgtgcccct cgaccaggtg ccggcccgcc
     4441 gtccgtacgg cctccgtcgt gcccttcgtc tgcgcctgct gaccggcggg cagctcccaa
     4501 agacccgcca gccggacctt cgtgacgaac tcccggtcca gcgcggacag cggtccgtac
     4561 tgcgtcgaca ccgtctgcgc gttcagtacg tccactccgg tgcccgaacg gtcggcgtac
     4621 gaccagatcg ggaacaccag cgccgccagg gtcgccgcca ggcccgtgat gatgagcccg
     4681 gttccgctga agatgcctct gccctggacg ggcggtcgcg gccgcatggg gtgcctcctg
     4741 ctcgcggcac tgcacgctag tgcgcttcgg tttgcgggac tggctggcat attactgcgc
     4801 ctgcgccgcc aactgccgta cgggggtaaa cggttgcaat gggagtatct cgggcgaaac
     4861 ggaaggggtg aaccagtcct tgaaacggga gaaatgcagt gaaagcagtg cgggaacgtt
     4921 gtgggagcga ttgcggtgcg cgtcgcgggc cgtgcggtgt taaccccggc acactcgggt
     4981 ggcccacggc ccaggcttcc tcaccgtcga atccacgtcc ggcccacccg tcccgcccgt
     5041 cccaaccgtg acttcggcct cggcgcgggg cccgagcggt ccagccacca gcgccgcagt
     5101 tcgcggcggg cccggccctc ggtcagccgt ccccccagga gcgtctgctc ggcgaagtcg
     5161 agggcgtcct gccggtagcc gccgtgcatc gggtggcttt gggcgtaggc gatgaacgcg
     5221 ttgcggtaac cgcttccgag aatgagcggc agctccgggg ccaccttcgc gaccacgtcc
     5281 gcccgcttcg ccgccagcgc ccgggcctgt acgccgagcc gcacccggtc gaaaccctcg
     5341 ggaaccggcg tcccggcgac cagtgcggac agcagcgccg cctgcgcgag cccgaggcgc
     5401 tgacgagtgg cctcgtcgag ggcggccggg cccggctccg agatcggcgg ggcgcctccg
     5461 tcccccgatg ctgtcctggt cccgataccc atcgcggtcg cggtcctggg catggtcctg
     5521 gtgccgatcc cggtcgcggt cctggtcccg acgcccgttc ccggcgtcgg ggcgtgctcc
     5581 cccgtggccg aagaagctcg cgcgggggca ccttcccgct cgcccgcctc gacggccgcc
     5641 ctgatcgtgc ccaactcccg ctccagctca gccggttcgg ggaagttctc gtcccgctcc
     5701 agcagcaccc ccggcggcga cacccgggac gcgaggtcgg tgaggatgtc gaggaccggc
     5761 cggggcaccg ggtgggcgtg actgtcgtgc cagacgccgt cccgctcgaa gccgcccgcc
     5821 acatggacgt aggcgatggc ctccaggggc agttcggcga gcgccttggc agggtcctcg
     5881 ccccggttga cgtggttggt gtgcaggttg gccacgtcga tgagcagccg tacgcccgtg
     5941 cggtcggcca gctcgtacag gaactgtccc tcggtcatct cctcgccggg ccacgcgatg
     6001 agcgcggcga tgttctcgac ggcgagcggc acgggcagcg cctcctgcgc gatgcgcacg
     6061 ttctcgcaca gcacgtcgag ggcgtcgcgg gtgcgcggca ccggcagcag gtgccccgcc
     6121 tcgagccgcg gggacgccgt cagggggccg cccgcgcgga cgaacgcgat gtgctcggtg
     6181 accagcggcg aacccagtgc ctcggcccgc tcggcgagcg cggccagccg tcccgcgtcg
     6241 ggtcgctccg catctcccag cccgagcgag acgccgtgcg ggatcacggt gaccccgcgc
     6301 tcgcgcagcc ggagcaggga gtcgggcaga tgcccggggc agaggttctc ggcgacggcc
     6361 tcgacccagt cgatgcccgg catgcgctcg acggcgtccg cgatctccgg ccgccagccg
     6421 atccccgtcc ccagtcgcct catggtcccc gcctccttag tgccccctgc gtggtgacgg
     6481 tggtatggcc ccgccatcga cgcccgaacc gcccgccacc ctccttcaga gcaacatttg
     6541 aggttcgggc gggaggaccg gggagccacg ggggcgccga ctggcggggc ggggcggggc
     6601 gggggatggc ctgcggtgag ctccgggact accgactacc gaatgcgggc gccgggctcc
     6661 ggagccaggg agccatggat cagcgggccc gtagggccgg gtggtcggcg accaccgtgc
     6721 aactgcccgg ggcgatctcg gtgaacccgg cgtcccgcac cagcggcagt ccgctctccg
     6781 tcagctcacg ccaccgtcgc ggatccgcgg tccgcacggc gagcggaaaa cccgcctcgc
     6841 gccaggcggt ccgctccgcg tccgacagct cccaccaggc gagctgcgcg ccgtgcccgg
     6901 cctgcgccat cgccttgccc gcggacatgc cgacctcggg gttctgccag atcacgggag
     6961 ccgtcgcgtc gatgtcgacc ggcggctcag gatcctcgag atcggttccg gaaacctgca
     7021 gcttcgccag gtccttgggc cagccgtcca gcggaacagg cgggaaaacc cttacttccg
     7081 ccgccttgcc catgaccgtg atgccgggca gcgcctccgc ccgccgccac tcggcgcccc
     7141 gcgcccgccg caccaccttg cggatccggg cgtcctgcca gtcacgcacg tccgccgccc
     7201 actcgccctc gcccagcgcc cgctcgtcgg cgaggagggt cagtacggct cgcgctgccg
     7261 tctccagcgc gtccgtgcgg gccggcgggt cggcacgctc gatacggacc accaaaggca
     7321 ggacgaactg cggagcctcg tcccgggtcg aacgttcgga acggaagggg ctgtcgccgg
     7381 gaacggccgg atcactggtc acgtcctcca gtctgccagg tggaagatcc aggcaaccgc
     7441 gagggaaagg acggtccgca tgccacgtga gctacggctg gaggccgtgg ggcgccgcta
     7501 cggcatgcgc ggcccctggg tgttccgcga cgtgcacctg aacccggcgt ccgccaccct
     7561 cacccgcgtc gacggcccca acggcaccgg gaagtccacc ctgctccgcc tcctcgcccg
     7621 catcgacgcc ccgaccgagg gcaaggtcac cggcgccccc cgaaccgcct acgttcccga
     7681 acgtttcccc gccgccctcc cgttcaccgc cctcggctac ctcacccacc tcggcgcggt
     7741 ccacggcctg tcccgtgcgg ccgccgagcg ggccgcgggc gactggctgg accgcttcgg
     7801 cgccaccgcc cacgcccaca ccccgatgag ccgcctctcc aagggcagca gccagaaggt
     7861 cgccgtggca caagcactcc tcgccgaacc ggagttgccg atcctggacg aggcctggac
     7921 cggactcgac accgacgcgc gcgccgaact cgaacgtgcc gtcgccgagc ggacggccgc
     7981 cggatcggcc gtcgtcttcg tggaccacga cccccgccgc ctcgcgggcc gggccgacgc
     8041 cgagtacacg gtccgcgacg gcactctcca ccgccgtgcc gaagcggagc cgaccgccgc
     8101 ccaagacggc ccgcacacga ccatcgaggt gcacggcccg accgccccgc tcccaccgga
     8161 agcactccgg ctggccacca ccaccgagga gactgccgac cgcacccacc ggctcaccgt
     8221 ccccgcctcc cactccgacg tcctgctccg cgccctgctg accgcccgcc caccgtggca
     8281 cgtggtgagc gtccgtccgg acgggagccg ccaccgatga ccgccctcct gcgctatcag
     8341 accgccctgc tcctgcgctc gcagcgctgg ctgcccccgt tcctcctgta cgccgtcttc
     8401 ctgggcgtcg gcgtgcaggg aggccagccg ctgctggact cgctcggcta tgcggcagcg
     8461 ttcctgctgc ccgtcgccgc ctggctggtc cggatctgtg tcaccaacga gcccccggcc
     8521 gcccgcaccg tcgtcgcggc tgcggtcggg cccgggcgcg cgcacctggc ctgtgtcctg
     8581 gtcgcgctga ccacggccgt cgtgttcggt acggcggcga cgacgacggt cacgctcatc
     8641 agcgacccgg tcggcaacga ccacgacaag cgcgttccgg tcctggaggc cgccggggcc
     8701 ggactgctcg ccgtcctgac ctgcgccctg ctcggcgcgg ccgtgggcgc gctcaccgcc
     8761 tggcccgtgc tgcgctcgcc cggccgcgcg gtccccgccc tgctgctggc ggccctgctg
     8821 gcgctggtgg tgaccggttc cccggcgcag gcggcggtga gcggcctggt cagcggctcc
     8881 cggtcggcga ccgtccatgt gccgctcctg cccctggccg cggcagccct gctgagcgcg
     8941 gtggcgacgg ctgtggcgtg cgcgctcacg tcacgtcgag caccgtgagg cggggaggtc
     9001 cgggggaagc ggttacgcgg cttgcgcggc aacaaacgaa cgggcgtcgg tgatgtgtgc
     9061 atcggtaacc gattacgggg gtgggtcagc gcacgggcgc cggtaaccga ttgcaggggt
     9121 tggcgaacga acgggcgcca gtaaccgatt gcgcgtcgat gagacgatca ggcgccggga
     9181 agcggttacg cgccgaacag acactcacgc gacggtaacc ggttacgcgt gcgcgaggcg
     9241 atcgcacgtc ggtaagcgat ctcgaatcag cgaccgggcg cggcgctcgt cggcggccca
     9301 cccgccggcg agcggccgcc ttccggagca cgcgacgccc ctgacccggg cgggcaaccg
     9361 gttgctcgcc cggaaagcaa ccggttgtgc ggagaaaacg ggtctcgcga gcgagcacac
     9421 gggcactccg agcagccggt caccccgagc acgccgtccg ctgggcctcc tgccactcgc
     9481 acaccgggca cagcgtgatc cccttgtacg actccggata ctccgtcggc ttccggcaca
     9541 gcacgcactc cgcgtacggc ggcccctcgg ccctgggcgg cgtggtggcg tcgatcaggc
     9601 agtactcgcg gtcactgtcg tcgctcatac ctccagcgta cgtaagcgac gacggcgtac
     9661 gcctcgcagg cggaccgacg tgcgaggcgg gcgaggtcca ggtgaccggc ggggtcgggc
     9721 cggtgaggcg tacggggttc accgccgtcc gctcgccgcc ccgatcagct cggacacctt
     9781 cacgaaccgg taccccttac ggcgcagttc cgggacgatc gtccgcaccg cctgctcggt
     9841 cgtcggggcg gcgctgcgcg tgcagtgcat gacgacgacg gagccgggcc ggaccccctc
     9901 cagcacctgc cgggccaccg cgtccgcgtc ggtcgcgaac gcgtccccgc tcaccacgtc
     9961 ccactgcacg gccgtcacgc ccatgccgct caccgcgcgc agcgcccgcc ggtcgtagca
    10021 accgccggga aatcggaagt agggcatcgc gtccggcacc ccggccctgc ggaaggccgt
    10081 gaacgcccgc tccacgtcgg cgcgcatcct gtcctcgggg acggtcggca ggccgtagca
    10141 gtcgtcggtg aaggcgtagt ggctgtagga gtggttggcg acctcgaagc cggggtcgtg
    10201 gccgagggag cgggcctggt ccgggtactc ctcggcccag cggccggtca tgaacaccgt
    10261 ggccggcacc ttcagcgcgc gcagtgccgc gatcagcccc gggttgtcga agtgctcgcc
    10321 ggccgccgcg cgggccccct ggtcggccgt catgtcggcg tcgaaggtga gggcgaccct
    10381 cttgtcggcg gtgcggggcg cgttgctgaa cacgggggtg agaccggccg gaccgggggc
    10441 cagcgtgggg ggccgggcgg gggcgaggga agtgtgcgcg ggggccgccg ggcgcagggc
    10501 acgtggagcg tgggggctgc cgcaggcgct gaggggggcg agggcggccc cgagagcgca
    10561 gagggcggcg aggcggcgta cgggaaggat catcatatga agttatgagg gttgtgccgg
    10621 catttgttgc gatgggtgac cgcgcctccg gtcgttcacc cgcccgggct ctcgatcctt
    10681 cgggaagcgc cccggctctc ggtccccggg acgcccccgg gcgggcggag ccgcgaagcc
    10741 ttcgcctccc cggcgcttcc ccgtgacgtc gcggcgcggt cgagcgggcc gggagctgcc
    10801 ccgccggggg ccccggacgc cgcacgcgcc ggacgccccg gaagcggtac gcgcgccgga
    10861 aacggcacgc gccggacgcg ccggggggcc ccgccgtgtc ggggccccgc ctcgcggatc
    10921 cggactagcg ccgccagggc cccgtgacgg cgaaggtcgt ccctggggtg tagcagttga
    10981 cgtacatcgt gtcgccgtcg ggggagaagg tgacgccggc gaactcgccc cactcggggg
    11041 cctgcggcgt tccgatgttc tggcggcccc gcgccatcgc gtacacctcg ccgcccctgg
    11101 tcacgccgaa gacgtgctgg gcgccgttgc cgtcctcgca gaccatcagg ccgccgctgg
    11161 gcgcgaggca gatgttgtcc ggggactcgc ccgggagctg gatgtccgtg tccgggccga
    11221 aggcgatgac cagggtgagg cggcggagct tggggtcgta ccgccagatc tggccgaagt
    11281 ggtcggccgc cgagccgtcc gagccgcgcg cgaacgacga cacgaagtac acgcagtgcc
    11341 cgccccagta acagccctcg agcttctggg cgtgggtgat gcccttgggg ccgaagtcct
    11401 gcagacggac cggggtttcg acggcgaggg ggtccggtac gtc
//
