LOCUS       NZ_LMEQ01000025         5682 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root1310 contig_31, whole genome shotgun sequence.
ACCESSION   NZ_LMEQ01000025
VERSION     NZ_LMEQ01000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root1310
  ORGANISM  Streptomyces sp. Root1310
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140604
            Assembly Name                     :: Root1310
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426425.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:14:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,470
            CDSs (total)                      :: 8,385
            Genes (coding)                    :: 8,123
            CDSs (with protein)               :: 8,123
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 98 of 262
            Pseudo Genes (incomplete)         :: 219 of 262
            Pseudo Genes (internal stop)      :: 20 of 262
            Pseudo Genes (multiple problems)  :: 64 of 262
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:32:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5682
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEQ01000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5682
                     /collection_date="2012"
                     /country="Germany: Cologne"
                     /db_xref="taxon:1736452"
                     /host="Arabidopsis thaliana"
                     /isolation_source="root"
                     /lat_lon="50.958 N 6.856 E"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. Root1310"
                     /strain="Root1310"
                     /submitter_seqid="contig_31"
     region          1..5682
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..5682
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5682
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3718:>5682](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3719..>5682
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            14..>688
                     /locus_tag="ASD48_RS45015"
     CDS             14..>688
                     /NRPS_PKS="Domain: PKS_DH (1-140). E-value: 1.6e-55. Score:
                     179.9. Matches aSDomain:
                     nrpspksdomains_ASD48_RS45015_PKS_DH.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /locus_tag="ASD48_RS45015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyketide synthase dehydratase domain-containing
                     protein"
                     /protein_id="WP_348983232.1"
                     /transl_table=11
                     /translation="MSLRTHPWLVDHAVAGTVLLPGTAFVELAIRAGDEAGCGRLDELT
                     LESPLVVPTEGTVSIQVRVGAEDEAGLRRVSVHSLTGEGQDWVRHADGVLAPDDTAEVF
                     DLAVWPPADAEPLPLEGFYEGLTESGYGYGPVFQGLRAAWRRGEELFAEVSLPEDVADG
                     ASGFGLHPALLDAALHVGLTDAEQAGRVRLPFVWSGVTLFAQGASSVRVRLLPTGEDSV
                     SVQ"
     aSDomain        17..433
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS45015_PKS_DH.1"
                     /evalue="1.60E-55"
                     /label="ASD48_RS45015_PKS_DH.1"
                     /locus_tag="ASD48_RS45015"
                     /protein_end="140"
                     /protein_start="1"
                     /score="179.9"
                     /tool="antismash"
                     /translation="SLRTHPWLVDHAVAGTVLLPGTAFVELAIRAGDEAGCGRLDELTL
                     ESPLVVPTEGTVSIQVRVGAEDEAGLRRVSVHSLTGEGQDWVRHADGVLAPDDTAEVFD
                     LAVWPPADAEPLPLEGFYEGLTESGYGYGPVFQGL"
     CDS_motif       35..109
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS45015_0001"
                     /evalue="1.40E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="ASD48_RS45015"
                     /protein_end="32"
                     /protein_start="7"
                     /score="33.6"
                     /tool="antismash"
                     /translation="WLVDHAVAGTVLLPGTAFVELAIRA"
     assembly_gap    689
                     /estimated_length=1
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1062..3722
                     /locus_tag="ASD48_RS27225"
                     /old_locus_tag="ASD48_41175"
     CDS             1062..3722
                     /NRPS_PKS="Domain: PKS_ER (129-433). E-value: 2.4e-121.
                     Score: 396.6. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27225_PKS_ER.1"
                     /NRPS_PKS="Domain: PKS_KR (442-622). E-value: 7.4e-65.
                     Score: 210.3. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27225_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (732-805). E-value: 2.1e-33. Score:
                     106.5. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27225_ACP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /locus_tag="ASD48_RS27225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41175"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_107104413.1"
                     /sec_met_domain="ADH_N (E-value: 3.3e-11, bitscore: 30.9,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 5.9e-20, bitscore:
                     59.4, seeds: 86, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 2.7e-66, bitscore:
                     210.5, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2e-17, bitscore:
                     51.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTFLQQWLSEERCTDSRLVVVTRGALGATPGDAVPDLAHAPVWG
                     LVRSAQTENPGRFVLVDLEAGTDADAALAEVLPRVLGSGEPQVVVRAGRALAARLTRST
                     TARSSTDGALVPPVGTSWRLDLTTPGTLENLTLVPCTPEPLGPLDVRIAVRAAGLNFRD
                     VLIALGMYPDPGAMGGEAAGVVTEVGSEVTGLEPGDRVMGIVPLSFGPDAVTDSRTVVR
                     IPEGWTFEQAASVPVAFVTAYYGLVDLAGVRAGESVLVHAAAGGVGMAAVQLARHLGAE
                     VFGTASPAKWDTLRAGGLDAGHIASSRTLDFEDTFRTATGGRGVDVVLNSLAGEFVDAS
                     LRLLPRGGRFVEMGKADIRDAEQVAAEHSGVAYRFYEMMEAGPQRTQEILREIVALFER
                     GVLTQLPVTAWDVRRAAEAFRFVSQARHIGKNVLTMPPVWDPDGTVLITGGTGALGALV
                     ARHVVAEHGIRHLLLAGRRGVEAPGARELLAELEAAGADVSVVACDVADRDAVAELVAK
                     VPSEHPLTAVVHTAGVLADGTIAALDAERVDTVMRPKVDAVLNLHEATRDLDLAGFVLF
                     SSASGALGSPGQGNYAAANSFLDAFAHHRRVQGLPALSLAWGLWAEASGMTGEFKGDDV
                     ARISRSGLAPLTNAQGMALLDTSLTLDEALLVPVRLDPAGLRAQAGAGTVPSLLRGLVR
                     TPGRRVATSKAGRSSGQEVSALLQRLVQLSHEDRTRALLDLVRSHAATTLGHAKPDAVE
                     AERGFLDLGFDSLTAVELRNRLNTAVGLRLPATLIFDHPTPAALAHHLRTELVPDEGEA
                     TGDAPRFTSDSGAGDEGRSAEETTLAVDDMDIADLIRMAHGELGTTEQQQADASPQFEA
                     PGHNGGPGRSEGQSS"
     aSDomain        1449..2360
                     /ASF="ER domain putatively catalyzing 2S-configuration
                     product formation"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27225_PKS_ER.1"
                     /evalue="2.40E-121"
                     /label="ASD48_RS27225_PKS_ER.1"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="433"
                     /protein_start="129"
                     /score="396.6"
                     /tool="antismash"
                     /translation="GTLENLTLVPCTPEPLGPLDVRIAVRAAGLNFRDVLIALGMYPDP
                     GAMGGEAAGVVTEVGSEVTGLEPGDRVMGIVPLSFGPDAVTDSRTVVRIPEGWTFEQAA
                     SVPVAFVTAYYGLVDLAGVRAGESVLVHAAAGGVGMAAVQLARHLGAEVFGTASPAKWD
                     TLRAGGLDAGHIASSRTLDFEDTFRTATGGRGVDVVLNSLAGEFVDASLRLLPRGGRFV
                     EMGKADIRDAEQVAAEHSGVAYRFYEMMEAGPQRTQEILREIVALFERGVLTQLPVTAW
                     DVRRAAEAFRFVSQARHIGKNVL"
     aSModule        1449..3476
                     /domains="nrpspksdomains_ASD48_RS27225_PKS_ER.1"
                     /domains="nrpspksdomains_ASD48_RS27225_PKS_KR.1"
                     /domains="nrpspksdomains_ASD48_RS27225_ACP.1"
                     /incomplete
                     /locus_tags="ASD48_RS27225"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1533..1568
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27225_0001"
                     /evalue="1.10E-04"
                     /label="PKSI-ER_m1"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="169"
                     /protein_start="157"
                     /score="15.0"
                     /tool="antismash"
                     /translation="GLNFRDVLIALG"
     CDS_motif       1590..1628
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27225_0002"
                     /evalue="2.30E-03"
                     /label="PKSI-ER_m2"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="189"
                     /protein_start="176"
                     /score="11.0"
                     /tool="antismash"
                     /translation="MGGEAAGVVTEVG"
     CDS_motif       1755..1799
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27225_0003"
                     /evalue="5.90E-06"
                     /label="PKSI-ER_m4"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="246"
                     /protein_start="231"
                     /score="19.4"
                     /tool="antismash"
                     /translation="AASVPVAFVTAYYGL"
     CDS_motif       1824..1886
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27225_0004"
                     /evalue="6.00E-10"
                     /label="PKSI-ER_m5"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="275"
                     /protein_start="254"
                     /score="31.3"
                     /tool="antismash"
                     /translation="GESVLVHAAAGGVGMAAVQLA"
     CDS_motif       2019..2057
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27225_0005"
                     /evalue="3.80E-06"
                     /label="PKSI-ER_m7"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="332"
                     /protein_start="319"
                     /score="19.1"
                     /tool="antismash"
                     /translation="TGGRGVDVVLNSL"
     CDS_motif       2313..2357
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27225_0006"
                     /evalue="3.00E-04"
                     /label="PKSI-ER_m9"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="432"
                     /protein_start="417"
                     /score="13.9"
                     /tool="antismash"
                     /translation="AFRFVSQARHIGKNV"
     aSDomain        2388..2927
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27225_PKS_KR.1"
                     /evalue="7.40E-65"
                     /label="ASD48_RS27225_PKS_KR.1"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="622"
                     /protein_start="442"
                     /score="210.3"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="GTVLITGGTGALGALVARHVVAEHGIRHLLLAGRRGVEAPGAREL
                     LAELEAAGADVSVVACDVADRDAVAELVAKVPSEHPLTAVVHTAGVLADGTIAALDAER
                     VDTVMRPKVDAVLNLHEATRDLDLAGFVLFSSASGALGSPGQGNYAAANSFLDAFAHHR
                     RVQGLPALSLAWGLWAE"
     CDS_motif       2391..2492
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27225_0007"
                     /evalue="4.60E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="477"
                     /protein_start="443"
                     /score="25.7"
                     /tool="antismash"
                     /translation="TVLITGGTGALGALVARHVVAEHGIRHLLLAGRR"
     CDS_motif       2721..2741
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27225_0008"
                     /evalue="1.10E+01"
                     /label="PKSI-ER_m7"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="560"
                     /protein_start="553"
                     /score="-0.3"
                     /tool="antismash"
                     /translation="KVDAVLN"
     CDS_motif       2778..2879
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27225_0009"
                     /evalue="9.60E-22"
                     /label="PKSI-KR_m4"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="606"
                     /protein_start="572"
                     /score="69.6"
                     /tool="antismash"
                     /translation="FVLFSSASGALGSPGQGNYAAANSFLDAFAHHRR"
     aSDomain        3258..3476
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27225_ACP.1"
                     /evalue="2.10E-33"
                     /label="ASD48_RS27225_ACP.1"
                     /locus_tag="ASD48_RS27225"
                     /protein_end="805"
                     /protein_start="732"
                     /score="106.5"
                     /tool="antismash"
                     /translation="LDLVRSHAATTLGHAKPDAVEAERGFLDLGFDSLTAVELRNRLNT
                     AVGLRLPATLIFDHPTPAALAHHLRTEL"
     gene            3719..>5682
                     /locus_tag="ASD48_RS27230"
                     /old_locus_tag="ASD48_41180"
     CDS             3719..>5682
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (37-463). E-value:
                     1.1e-185. Score: 609.6. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27230_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009719941.1"
                     /locus_tag="ASD48_RS27230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41180"
                     /product="type I polyketide synthase"
                     /protein_id="WP_157880705.1"
                     /sec_met_domain="mod_KS (E-value: 9.7e-236, bitscore:
                     770.0, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 7.5e-96, bitscore:
                     308.2, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 9.7e-32, bitscore: 98.2,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDASTDKVVGALRASLKETERLRLQNRKLEDAAREPIAIVGMAC
                     RYAGGVRSPEDLWRLVDEGRDAVSGVPEDRGWDMDTLYDPDPDAPGRTYVREGGFLSDA
                     GDFDADFFAVPPREALAMDPQQRLLLETSWEAIERAGITPRSLRGKHVGVYYGTGWSDY
                     VTRLHQPPAEVEGYFVTGSTHSVIAGRISYVFGLEGPAVTVDTACSSSLVSLHLAGQAL
                     RAGECSLALAGGVTVMASPKVFTEFSRLRGLAEDGRCKAFSASADGFGPAEGVGVLLLE
                     RLSDARRNGHEVLAVLRGSAVNQDGASSGLTAPNGPSQQRVIRAALANARLNPEDVDVV
                     EAHGTGTPLGDPIEAQALLATYGQGRPQGRPLWLGSIKSNIAHTQAAAGVAGVIKMVQA
                     MRHGVLPKTLHVDEASPHVDWSSGAVELLAEAREWPEVDRPRRAGVSSFGISGTNAHVI
                     VEQAPESAVGAVAESGVRVPVPVVPWGLAGQAERLAAFIEDRPETDLVDVGFSLVGSRS
                     VWEHRAVVLGRDRDALLGGLRELPVRGVAGEGMRVAFAFSGQGAQRNGMGAELASAYPL
                     FGEVLGEVGNALGLDPGVFGDGERLGRTEVTQAALFAFEVAVVRLLESFGVRPDVLIGH
                     SIGEIAAAYVAGVFSLGDAA"
     aSDomain        3830..5107
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27230_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.10E-185"
                     /label="ASD48_RS27230_PKS_KS.1"
                     /locus_tag="ASD48_RS27230"
                     /protein_end="463"
                     /protein_start="37"
                     /score="609.6"
                     /tool="antismash"
                     /translation="IAIVGMACRYAGGVRSPEDLWRLVDEGRDAVSGVPEDRGWDMDTL
                     YDPDPDAPGRTYVREGGFLSDAGDFDADFFAVPPREALAMDPQQRLLLETSWEAIERAG
                     ITPRSLRGKHVGVYYGTGWSDYVTRLHQPPAEVEGYFVTGSTHSVIAGRISYVFGLEGP
                     AVTVDTACSSSLVSLHLAGQALRAGECSLALAGGVTVMASPKVFTEFSRLRGLAEDGRC
                     KAFSASADGFGPAEGVGVLLLERLSDARRNGHEVLAVLRGSAVNQDGASSGLTAPNGPS
                     QQRVIRAALANARLNPEDVDVVEAHGTGTPLGDPIEAQALLATYGQGRPQGRPLWLGSI
                     KSNIAHTQAAAGVAGVIKMVQAMRHGVLPKTLHVDEASPHVDWSSGAVELLAEAREWPE
                     VDRPRRAGVSSFGISGTNAHVIVEQAP"
     CDS_motif       4085..4111
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27230_0001"
                     /evalue="8.30E-03"
                     /label="PKSI-KS_m2"
                     /locus_tag="ASD48_RS27230"
                     /protein_end="131"
                     /protein_start="122"
                     /score="9.1"
                     /tool="antismash"
                     /translation="DPQQRLLLE"
     CDS_motif       4313..4360
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27230_0002"
                     /evalue="1.10E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="ASD48_RS27230"
                     /protein_end="214"
                     /protein_start="198"
                     /score="20.5"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVS"
     CDS_motif       4736..4780
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27230_0003"
                     /evalue="1.10E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="ASD48_RS27230"
                     /protein_end="354"
                     /protein_start="339"
                     /score="14.1"
                     /tool="antismash"
                     /translation="VEAHGTGTPLGDPIE"
     CDS_motif       4841..4885
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27230_0004"
                     /evalue="4.50E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="ASD48_RS27230"
                     /protein_end="389"
                     /protein_start="374"
                     /score="9.8"
                     /tool="antismash"
                     /translation="GSIKSNIAHTQAAAG"
     CDS_motif       5378..5428
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27230_0005"
                     /evalue="5.30E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="ASD48_RS27230"
                     /protein_end="570"
                     /protein_start="553"
                     /score="15.8"
                     /tool="antismash"
                     /translation="FAFSGQGAQRNGMGAEL"
     CDS_motif       5594..5680
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27230_0006"
                     /evalue="9.40E-16"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="ASD48_RS27230"
                     /protein_end="654"
                     /protein_start="625"
                     /score="49.9"
                     /tool="antismash"
                     /translation="GVRPDVLIGHSIGEIAAAYVAGVFSLGDA"
ORIGIN
        1 gctgacgggt cgggtgtcgt tgcggacgca tccgtggctg gtggatcacg cggtggcggg
       61 gacggtgctg ctgccgggta cggcgttcgt ggagctggcg atccgggccg gtgacgaggc
      121 cggctgcgga cgcctcgacg aactcaccct cgaatcgccc ctggtggtgc ccacggaggg
      181 aacggtctcc atccaggtgc gggtcggggc cgaggacgag gcggggctcc ggcgggtcag
      241 cgtgcactcc ctcacggggg aggggcagga ctgggtccgc catgccgacg gtgtgctcgc
      301 cccggacgac acggcggagg tgttcgacct cgcggtgtgg cccccggccg acgccgagcc
      361 gcttccgctg gagggcttct acgagggcct gaccgagtcc ggctacggct acgggccggt
      421 gttccagggc ctgcgggcgg cctggcgacg cggcgaggaa ctcttcgccg aggtgagcct
      481 ccccgaggac gtcgcggacg gtgcgtccgg cttcggcctc cacccggcgc tgctggacgc
      541 ggctctccat gtcgggctca cggacgcgga acaggccggc cgagtgcggt tgccgttcgt
      601 gtggagcggg gtgacgctgt tcgcccaggg cgcctccagc gtgcgcgtgc ggctgctgcc
      661 cacgggcgag gactccgtct ccgtacagnc agggcgcctc cagcgtgcgc gtgcggctgc
      721 tgcccacggg cgaggactcc gtctccgtac aggtcgccga cgtcgaaggc actccggtcg
      781 cgtccgccga cgccgtcgtc acccgccttg cggtcccggg ccagttcagc cacccgtcca
      841 gtggggaaca ggactcgctc ttcaggatcg actggacacc gctgaagccg ggcgacgagg
      901 tgtcaccgga ccggtggacg gtgctggacg agggcgccgc cggaccggac gccgagagcc
      961 tcccggaatt cgtggtcctt ccctgtggga cgggagcctc cgagcccgcc tccggcgcga
     1021 gcggcggtct cacgcacgcg gtacgtcaag ccaccgccgg aatgctgacg ttccttcagc
     1081 agtggctctc cgaggaacgc tgcaccgact cacgcctggt cgtggtgacc cggggagcgc
     1141 tgggcgcgac ccccggcgat gccgtaccgg atctggcgca tgccccggtg tggggtctgg
     1201 tgcggtcggc gcagaccgag aatccgggcc ggttcgtgct cgtggacctg gaagccggta
     1261 ctgacgcgga cgccgccctg gccgaggtgc tgcccagggt gctgggctcc ggtgaaccgc
     1321 aggtggtggt gcgtgccgga cgcgcgctgg cggcgcgtct gacgcggtcg acgaccgcgc
     1381 ggtcgtcgac ggacggtgcg ctggtgccac cggtcggcac atcctggcga ctggacctga
     1441 cgaccccggg cacactggag aacctgacgc tggtgccctg cacgccggag ccgttgggtc
     1501 cgctcgatgt gcggatcgcc gtacgcgctg ccggcctcaa cttccgtgac gtgctgatcg
     1561 ctctcggcat gtacccggac cccggcgcga tgggcggcga ggcggccggt gtcgtcacgg
     1621 aggtcggttc cgaggtcacc ggcctggaac cgggggaccg cgtgatgggc atcgtcccgc
     1681 tgtccttcgg accggacgcg gtcaccgact cccggaccgt ggtgcgcatc cccgagggct
     1741 ggaccttcga gcaggccgcg tcggtcccgg tcgccttcgt caccgcctac tacgggctgg
     1801 tggaccttgc cggggtgcgg gcgggcgaat cggtgctggt gcacgcggcg gcgggcggtg
     1861 tggggatggc cgcggtgcag ctcgcccggc acctgggcgc ggaggtcttc ggtacggcga
     1921 gtccggcgaa gtgggacacg ctgcgagccg gcggtctcga cgccggacac atcgcgtcct
     1981 cccggacgct cgacttcgag gacacgttcc gcacggccac gggcgggcgc ggcgtggacg
     2041 tcgtgttgaa cagcctggcc ggtgagttcg tcgacgcgtc gctgcggctg ctgccgcgcg
     2101 gcggacgctt cgtcgagatg ggcaaggccg acatacggga cgcggagcag gtcgctgccg
     2161 agcactccgg cgtcgcctac cgcttctacg agatgatgga agcgggcccg cagcgcactc
     2221 aggagatcct ccgcgagatc gtggcactct tcgagcgtgg ggtgctgacg cagctgccgg
     2281 tgacggcgtg ggacgtgcgg cgggcggcgg aggccttccg gttcgtcagc caggcgcggc
     2341 atatcggcaa gaacgtgctg acgatgccgc ccgtgtggga cccggacggc accgtgttga
     2401 tcacgggcgg cacgggcgcc ctgggcgcgt tggtggcccg gcacgtggtg gccgaacacg
     2461 ggatacgcca cctgctgctg gccggccgga ggggcgtgga ggcgcccgga gcacgggagc
     2521 tgctggccga actggaagcg gccggtgcgg atgtgtcggt cgtcgcgtgt gacgtggccg
     2581 accgggacgc ggtggccgag ctggtcgcca aggtgccgtc cgaacacccc ctgaccgcgg
     2641 tcgtccacac cgcgggcgtc ctggcggacg gcacgatcgc cgcactggac gcggagcgcg
     2701 tcgacacggt gatgcggccg aaggtcgatg ccgtgctcaa cctgcacgag gcgaccaggg
     2761 atctggacct cgccggattc gtcctgttct cctcggcctc cggtgccctc ggcagtccgg
     2821 gccagggcaa ctacgcggca gccaactcct tcctggacgc cttcgcccac caccggaggg
     2881 tccagggcct gcccgccctc tcgctcgcct ggggcctgtg ggccgaagcc agcggcatga
     2941 ccggggagtt caagggcgac gacgtcgcac ggatctcccg ctccgggctc gcccccctca
     3001 ccaacgcgca gggcatggca ctcctcgaca cctccctcac cctggacgag gcactgctcg
     3061 ttcccgtccg tctcgacccc gccggactgc gcgcgcaagc cggcgcgggc accgtgccgt
     3121 ccctgctccg gggtctcgtg cgcacacccg gtcgccgcgt cgccaccagc aaggccggcc
     3181 gatcctccgg ccaggaggta tcggccctgc tccagcgcct cgtacagctc tcccacgagg
     3241 accggacgcg cgccctgctg gacctcgtac gcagccacgc ggcaaccacg ctcggccacg
     3301 ccaagcccga cgccgtggaa gccgaacggg gcttcctcga cctgggcttc gactccctga
     3361 ccgcggtgga actgcgcaac cgcctcaaca cggccgtcgg tctgcgcctc cccgccaccc
     3421 tcatcttcga ccatccgacc cccgccgccc tcgcccacca cctgcgcacg gaactcgttc
     3481 ccgacgaggg cgaggcaacc ggcgacgccc ctcgcttcac ctcggacagc ggagcgggcg
     3541 acgaaggccg gagtgccgag gagacgaccc tcgccgtcga cgacatggac atcgccgacc
     3601 tgatccgcat ggcacacggc gaactcggca ccaccgaaca gcaacaggcc gatgcatcac
     3661 cacagttcga agcgcccggc cacaacggcg ggccgggacg atccgaaggg cagagctcgt
     3721 gactgacgcg tcgaccgaca aggttgttgg agccctccgg gcgtcgttga aggagaccga
     3781 acggctccgg ctgcagaacc ggaagctgga ggacgcggcc cgggagccca tcgccatcgt
     3841 cggcatggcc tgccgctacg cgggcggcgt ccgatccccc gaggacctgt ggcgactcgt
     3901 cgacgaggga cgggacgcgg tctccggcgt ccccgaggac cgcggctggg acatggacac
     3961 cctctatgac cccgacccgg acgcgcccgg caggacctac gtccgcgaag gcggattcct
     4021 gagcgacgcc ggagacttcg acgcggactt cttcgccgtc cccccgcgcg aggcactcgc
     4081 catggacccc cagcagcgcc tgctgctgga gacctcctgg gaagcgatcg agcgtgccgg
     4141 catcaccccc aggtcactgc gcggcaaaca cgtcggcgtc tactacggca ccggctggtc
     4201 cgactacgtc acccgactgc accagccccc cgcggaggtc gaaggatact tcgtcaccgg
     4261 cagcacccac agcgtcatcg cgggccgcat ctcctacgtc ttcggactgg agggcccggc
     4321 tgtcaccgtg gacacggcct gctcgtcgtc cctggtctcc ctccacctcg ccggccaggc
     4381 gctgcgcgcg ggcgaatgct cgctcgccct cgcgggcggt gtgaccgtga tggccagccc
     4441 caaggtcttc accgagttca gccgtctgcg cggactcgcc gaggacggcc gctgcaaggc
     4501 cttctcggca tccgccgacg gtttcggccc ggccgagggg gtcggcgtcc tcctgctgga
     4561 gcgcctgtcg gacgcccgcc gcaacgggca cgaggtgctg gccgtactgc ggggcagcgc
     4621 cgtcaaccag gacggcgcca gcagcggact caccgccccc aacggccctt cccaacagag
     4681 ggtgatccgc gccgcgttgg ccaacgcgcg gctgaacccg gaagacgtcg acgtcgtcga
     4741 ggcccacggc accggcacac cgctgggcga cccgatcgag gcgcaggcac tgctggccac
     4801 ctatggccaa ggacgcccgc aaggacgccc gttgtggctc gggtccatca agtcgaacat
     4861 cgcgcacacg caggcggcgg cgggtgtcgc cggggtcatc aagatggtcc aggcgatgcg
     4921 gcacggagta ctgccgaaga cactgcacgt cgatgaggcg tcgccgcatg tggactggtc
     4981 gtcgggcgcg gtggagttgc tggccgaggc gcgggagtgg ccggaggtcg atcgtccgcg
     5041 gcgggctggt gtgtcgtcgt tcgggatcag tgggacgaac gcgcacgtga tcgtggagca
     5101 ggcgcccgag tccgctgtcg gggcggttgc cgagtcgggt gtccgtgtcc cggttccggt
     5161 ggtgccgtgg gggctggccg ggcaggctga gcggttggcc gcgttcatcg aggatcgtcc
     5221 cgagacggac ttggtggatg tggggttctc gctggtcggg tcgcgttcgg tgtgggagca
     5281 ccgggcggtg gtgctggggc gcgatcggga cgcattgctg ggtgggttgc gggagttgcc
     5341 ggtgcggggt gtggccggtg agggcatgcg ggtggcgttc gccttctcgg gccagggggc
     5401 gcagcgaaac ggtatgggtg ccgaactggc gtctgcgtat cccttgttcg gcgaggtgct
     5461 gggtgaggtg ggtaacgcgc tgggtctgga tcccggcgtg ttcggggacg gtgagcggtt
     5521 gggccggacg gaggtgacgc aggctgcgct gttcgctttt gaggtggcgg tggtgcggtt
     5581 gttggagagt ttcggggtgc ggcctgatgt gttgatcggg cattcgatcg gggagatcgc
     5641 ggctgcgtat gtggcggggg tgttctcgct gggggatgcg gc
//
