LOCUS       NZ_LMEQ01000024        10632 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root1310 contig_30, whole genome shotgun sequence.
ACCESSION   NZ_LMEQ01000024
VERSION     NZ_LMEQ01000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root1310
  ORGANISM  Streptomyces sp. Root1310
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10632)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 10632)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140604
            Assembly Name                     :: Root1310
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426425.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:14:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,470
            CDSs (total)                      :: 8,385
            Genes (coding)                    :: 8,123
            CDSs (with protein)               :: 8,123
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 98 of 262
            Pseudo Genes (incomplete)         :: 219 of 262
            Pseudo Genes (internal stop)      :: 20 of 262
            Pseudo Genes (multiple problems)  :: 64 of 262
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:32:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10632
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEQ01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..186)
                     /locus_tag="ASD48_RS45590"
                     /old_locus_tag="ASD48_41150"
     CDS             complement(<1..186)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASD48_RS45590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ASD48_41150"
                     /product="hypothetical protein"
                     /protein_id="WP_242432746.1"
                     /transl_table=11
                     /translation="MERVTDPQTTGLHLPQPRDHLLHSLRLTGDHHRRRTIDRGNRHTI
                     GETLKSGNDLRLTRLHR"
     source          1..10632
                     /collection_date="2012"
                     /country="Germany: Cologne"
                     /db_xref="taxon:1736452"
                     /host="Arabidopsis thaliana"
                     /isolation_source="root"
                     /lat_lon="50.958 N 6.856 E"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. Root1310"
                     /strain="Root1310"
                     /submitter_seqid="contig_30"
     region          1..10632
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..10632
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10632
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[5563:>10632](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5564..>10632
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            94..3543
                     /locus_tag="ASD48_RS27205"
                     /old_locus_tag="ASD48_41155"
     CDS             94..3543
                     /NRPS_PKS="Domain: PKS_DH (201-368). E-value: 5e-64. Score:
                     207.6. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27205_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (689-868). E-value: 5.8e-63.
                     Score: 204.1. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27205_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (983-1055). E-value: 4.9e-32. Score:
                     102.1. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27205_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (1066-1144). E-value:
                     2.6e-15. Score: 49.0. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27205_PKS_Docking_Cterm.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /locus_tag="ASD48_RS27205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41155"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_242432745.1"
                     /sec_met_domain="PKS_AT (E-value: 6e-42, bitscore: 132.1,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.2e-66, bitscore:
                     212.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 4.5e-08,
                     bitscore: 20.6, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.3e-17, bitscore:
                     52.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSGEAEAVEQVVARLGEVKSRRLRVSHAFHSPLMEPMLADFREV
                     VEQVTFQEPTVPVVSNVSGRFAEPGELTTPDYWVRHVREAVRFGDGVGALAADGVGVLV
                     EVGPDSVLTALARESFDGGDGLRAVPLLRKDRPEPETLLTGVAQAFTHGVEVDWSAMLP
                     GGRPVELPTYAFQRRRYWLEDADSAGGDPAALGLTAADHPLLGAAVLLAGDQGALLTGH
                     LSVRTQPWLADHAVAGTVLLPGTGFVELAIRAGDEVGCGRLEELTLEAPLVIPEGGGVP
                     VQVRVGPEEVPGRRPVTVHSSAAEGEEWVRHAGGVLTADTADTADASEEASGLTSWPPA
                     DAEPVDLEGFYERLSTSGYGYGPAFRSLRAAWRRGDEIFAEVTLPEQQDVSGFGVHPAL
                     LDAALHAVMATGDGHEGIRLPFSWSGVSLFAAGASNVRVRLAPAGPDAVSVEVADAKGQ
                     PVVSVDALVSREVQADQLPRDQRARDKDQGGLFALEWTPAPVVDAPPAHWTPLGADEHQ
                     VVEKQAEFVVLPWTPPTDADDLPGAVREVTGQVLEAVRRWLSDEYQDQHQDQDQDQNQD
                     QNQGQPQDGASAASKLVVVTHGAVSAGPDDPVPDLAHAAVWGLVRSAQSEHPGRFVLVD
                     IDERDDWQSVLGSALRRDEPQMVVRGGTVLTPRLVGVAPEEDPNSAKEWNWDPDGTVVI
                     TGGTGALGRLLARRLVKDRGMRRLVLASRSGPEADSATELREELESEGARVSVVACDVT
                     DPDAVAELIAKVPEEHPLTAVVHTAGVLDDGVIETLTRDRLDVVLRAKTDAAVHLHRAT
                     RDLPLVDLVLFSSVAGVLGAPGQANYAAANTFLDALAQHRRAQGLPAVSLAWGLWAQDG
                     GMAGELAGENLERVARTGLDPLTPEQGLDLFETVVRTNRATGRALAIPLRLNTGVLRAR
                     GDSAGLPVLLRGLVSGPVRRTAHSTGATGGIGTEVSLAHRLEGIPESEQERVVLDLVRE
                     HVASVLGHPSVTGINPEQAFQDLGFDSLTAVELRNRLGAATGLRLPATAVFDHPSPAAL
                     GRYVRARVAPAPADPTTRLLTDLDRFEAELLGTTPDDDDSVRITNRLRAVLSKWTEQRR
                     RVDASDQDQTDRSAVGDHLATATAEEIFDFIDRGLGRKKGDS"
     CDS_motif       322..357
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27205_0001"
                     /evalue="4.40E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="ASD48_RS27205"
                     /protein_end="88"
                     /protein_start="76"
                     /score="16.7"
                     /tool="antismash"
                     /translation="YWVRHVREAVRF"
     aSDomain        697..1197
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27205_PKS_DH.1"
                     /evalue="5.00E-64"
                     /label="ASD48_RS27205_PKS_DH.1"
                     /locus_tag="ASD48_RS27205"
                     /protein_end="368"
                     /protein_start="201"
                     /score="207.6"
                     /tool="antismash"
                     /translation="HPLLGAAVLLAGDQGALLTGHLSVRTQPWLADHAVAGTVLLPGTG
                     FVELAIRAGDEVGCGRLEELTLEAPLVIPEGGGVPVQVRVGPEEVPGRRPVTVHSSAAE
                     GEEWVRHAGGVLTADTADTADASEEASGLTSWPPADAEPVDLEGFYERLSTSGYGYGPA
                     FRSL"
     aSModule        697..3258
                     /domains="nrpspksdomains_ASD48_RS27205_PKS_DH.1"
                     /domains="nrpspksdomains_ASD48_RS27205_PKS_KR.1"
                     /domains="nrpspksdomains_ASD48_RS27205_ACP.1"
                     /incomplete
                     /locus_tags="ASD48_RS27205"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       781..855
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27205_0002"
                     /evalue="7.70E-11"
                     /label="PKSI-DH_m2"
                     /locus_tag="ASD48_RS27205"
                     /protein_end="254"
                     /protein_start="229"
                     /score="34.4"
                     /tool="antismash"
                     /translation="WLADHAVAGTVLLPGTGFVELAIRA"
     aSDomain        2161..2697
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27205_PKS_KR.1"
                     /evalue="5.80E-63"
                     /label="ASD48_RS27205_PKS_KR.1"
                     /locus_tag="ASD48_RS27205"
                     /protein_end="868"
                     /protein_start="689"
                     /score="204.1"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVVITGGTGALGRLLARRLVKDRGMRRLVLASRSGPEADSATEL
                     REELESEGARVSVVACDVTDPDAVAELIAKVPEEHPLTAVVHTAGVLDDGVIETLTRDR
                     LDVVLRAKTDAAVHLHRATRDLPLVDLVLFSSVAGVLGAPGQANYAAANTFLDALAQHR
                     RAQGLPAVSLAWGLWA"
     CDS_motif       2164..2265
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27205_0003"
                     /evalue="4.80E-10"
                     /label="PKSI-KR_m1"
                     /locus_tag="ASD48_RS27205"
                     /protein_end="724"
                     /protein_start="690"
                     /score="32.0"
                     /tool="antismash"
                     /translation="TVVITGGTGALGRLLARRLVKDRGMRRLVLASRS"
     CDS_motif       2554..2652
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27205_0004"
                     /evalue="4.20E-22"
                     /label="PKSI-KR_m4"
                     /locus_tag="ASD48_RS27205"
                     /protein_end="853"
                     /protein_start="820"
                     /score="70.8"
                     /tool="antismash"
                     /translation="VLFSSVAGVLGAPGQANYAAANTFLDALAQHRR"
     aSDomain        3043..3258
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27205_ACP.1"
                     /evalue="4.90E-32"
                     /label="ASD48_RS27205_ACP.1"
                     /locus_tag="ASD48_RS27205"
                     /protein_end="1055"
                     /protein_start="983"
                     /score="102.1"
                     /tool="antismash"
                     /translation="LDLVREHVASVLGHPSVTGINPEQAFQDLGFDSLTAVELRNRLGA
                     ATGLRLPATAVFDHPSPAALGRYVRAR"
     aSDomain        3292..3525
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27205_PKS_Docking_Cterm.
                     1"
                     /evalue="2.60E-15"
                     /label="ASD48_RS27205_PKS_Docking_Cterm.1"
                     /locus_tag="ASD48_RS27205"
                     /protein_end="1144"
                     /protein_start="1066"
                     /score="49.0"
                     /tool="antismash"
                     /translation="LLTDLDRFEAELLGTTPDDDDSVRITNRLRAVLSKWTEQRRRVDA
                     SDQDQTDRSAVGDHLATATAEEIFDFIDRGLGR"
     gene            3546..>5396
                     /locus_tag="ASD48_RS27210"
                     /old_locus_tag="ASD48_41160"
     CDS             3546..>5396
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (3-30). E-value:
                     7.9e-18. Score: 55.7. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27210_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (35-462). E-value:
                     1.3e-186. Score: 612.6. Matches aSDomain:
                     nrpspksdomains_ASD48_RS27210_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717842.1"
                     /locus_tag="ASD48_RS27210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41160"
                     /product="type I polyketide synthase"
                     /protein_id="WP_199875983.1"
                     /sec_met_domain="mod_KS (E-value: 3.4e-238, bitscore:
                     778.5, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 6.6e-96, bitscore:
                     308.8, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASDEKLVEYLKWVTADLHETRERLQELESGSREPIAIIGMSCRY
                     PGDVRSPEELWELVRTGTDAVSDFPGNRGWDVEGLYDPDPERRGKLYAREGGFLHDADR
                     FDAGFFGISPREATAIDPQQRLLLETSWEAFERAGIAPDSLRGTRTGVFTGVMYDDYGV
                     RMRPMPEAFEGLLGSGSAGSVASGRVAYTFGLEGPAVTVDTACSSSLVAMHLASQALRD
                     GECSLALAGGVALMATPTIFIEFSRQRGLARDGRCKPFSDAADGTGWGEGVGVLLLERL
                     SDARRNGHRILAVLRGSAVNQDGASNGLTAPNGPSQQRVIETALANAQLSTNDVDAVEA
                     HGTGTSLGDPIEAQALLATYGQGRPEERPLWLGSVKSNIAHTQAAAGVAGVIKMVMALR
                     HGVLPKTLHVDAPSAHVDWAAGAVELLTESRDWPRTDGRPRRAGVSSFGISGTNAHVIV
                     EEAPESSADAVAESGVRVPVPVVPWVVSARGADGLAAQAERLAGFVEERAELDPVDVGF
                     SLAASRAVLERRAVVMGQDRDGLRDALRALAEGSPSAGVVSGVARGRARVAFGFSGQGA
                     QRVGMGAELASVYPVFAEALAEVSGALGLDPEVFGDVDRLG"
     aSDomain        3555..3635
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27210_PKS_Docking_Nterm.
                     1"
                     /evalue="7.90E-18"
                     /label="ASD48_RS27210_PKS_Docking_Nterm.1"
                     /locus_tag="ASD48_RS27210"
                     /protein_end="30"
                     /protein_start="3"
                     /score="55.7"
                     /tool="antismash"
                     /translation="DEKLVEYLKWVTADLHETRERLQELES"
     aSDomain        3651..4931
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS27210_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.30E-186"
                     /label="ASD48_RS27210_PKS_KS.1"
                     /locus_tag="ASD48_RS27210"
                     /protein_end="462"
                     /protein_start="35"
                     /score="612.6"
                     /tool="antismash"
                     /translation="IAIIGMSCRYPGDVRSPEELWELVRTGTDAVSDFPGNRGWDVEGL
                     YDPDPERRGKLYAREGGFLHDADRFDAGFFGISPREATAIDPQQRLLLETSWEAFERAG
                     IAPDSLRGTRTGVFTGVMYDDYGVRMRPMPEAFEGLLGSGSAGSVASGRVAYTFGLEGP
                     AVTVDTACSSSLVAMHLASQALRDGECSLALAGGVALMATPTIFIEFSRQRGLARDGRC
                     KPFSDAADGTGWGEGVGVLLLERLSDARRNGHRILAVLRGSAVNQDGASNGLTAPNGPS
                     QQRVIETALANAQLSTNDVDAVEAHGTGTSLGDPIEAQALLATYGQGRPEERPLWLGSV
                     KSNIAHTQAAAGVAGVIKMVMALRHGVLPKTLHVDAPSAHVDWAAGAVELLTESRDWPR
                     TDGRPRRAGVSSFGISGTNAHVIVEEAP"
     CDS_motif       3906..3932
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27210_0001"
                     /evalue="7.70E-03"
                     /label="PKSI-KS_m2"
                     /locus_tag="ASD48_RS27210"
                     /protein_end="129"
                     /protein_start="120"
                     /score="9.1"
                     /tool="antismash"
                     /translation="DPQQRLLLE"
     CDS_motif       4134..4181
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27210_0002"
                     /evalue="2.50E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="ASD48_RS27210"
                     /protein_end="212"
                     /protein_start="196"
                     /score="22.4"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       4557..4601
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27210_0003"
                     /evalue="1.00E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="ASD48_RS27210"
                     /protein_end="352"
                     /protein_start="337"
                     /score="14.2"
                     /tool="antismash"
                     /translation="VEAHGTGTSLGDPIE"
     CDS_motif       4662..4706
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27210_0004"
                     /evalue="2.70E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="ASD48_RS27210"
                     /protein_end="387"
                     /protein_start="372"
                     /score="10.5"
                     /tool="antismash"
                     /translation="GSVKSNIAHTQAAAG"
     CDS_motif       5256..5300
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS27210_0005"
                     /evalue="1.10E-04"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="ASD48_RS27210"
                     /protein_end="585"
                     /protein_start="570"
                     /score="14.9"
                     /tool="antismash"
                     /translation="FSGQGAQRVGMGAEL"
     assembly_gap    5397..5525
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5564..>10632
                     /locus_tag="ASD48_RS44875"
                     /old_locus_tag="ASD48_41165"
     CDS             5564..>10632
                     /NRPS_PKS="Domain: PKS_DH (193-364). E-value: 2.2e-63.
                     Score: 205.5. Matches aSDomain:
                     nrpspksdomains_ASD48_RS44875_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (748-928). E-value: 1.1e-63.
                     Score: 206.4. Matches aSDomain:
                     nrpspksdomains_ASD48_RS44875_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (1024-1110). E-value: 9.7e-33.
                     Score: 104.4. Matches aSDomain:
                     nrpspksdomains_ASD48_RS44875_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (1146-1540). E-value:
                     1.7e-99. Score: 325.6. Matches aSDomain:
                     nrpspksdomains_ASD48_RS44875_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717833.1"
                     /locus_tag="ASD48_RS44875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41165"
                     /product="type I polyketide synthase"
                     /protein_id="WP_242432744.1"
                     /sec_met_domain="PKS_AT (E-value: 5.2e-38, bitscore: 119.2,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1e-65, bitscore:
                     209.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.3e-17, bitscore:
                     51.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 3.7e-125, bitscore:
                     406.1, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 4.3e-95, bitscore:
                     306.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEEVVARLGKVKSKRLRVSHAFHSPLMEPMLADFRQVAEQITYNE
                     PQLPVVSNVSGRLAEPGELTTPDYWVRHVREAVRFGDGVGALAADGVGVLVEVGPDSVL
                     TALARESFDGGDGMRAVPLLRKDRPEPETLLTGVAQAFTHGVEVDWSALLPGGRRVELP
                     TYAFQQQSYWLEEPSRSAGDPAAFGLGAADHPLLTAAVGLADGQGVVLTGRLSLRTHPW
                     LADHAVAGTVLLPGTAFVELAIRAGDEAGCGRLEELALEAPLLMTEDDALAVQVRVGAP
                     DEAGRRPVGVYSRSGNSGDEQEWARHASGVLTADTPGEALDRTEGALDLTVWPPADTEP
                     IDLSGFYEASATSGYGYGPAFQGLRAAWRRGDEIFAEVSLPEHAEQAEQGERPEQGELP
                     GRSGQTDQDERASGFGMHPALLDAVLHAVIAAESRGARPRDPEPQGDVRLPFSWNGVSL
                     HAAGATTVRARIRLSGTDSVSLQVADGTGRPVMSVDALVSRPVPAEQLRHPASGDASGL
                     FDVEWVPVPAVPRTPEPERTWAVLGRDGLGLDDLAQDADTFPAFVVFPCVTTVTATATD
                     DLTATDDLAAATDGPAATDDSTVIDGLAVAARAATVQALELVRRWLSDERYADSQLVVV
                     TSGAVATSSDAPVADLVHAPVWGLVRSAQAEHPGRFVLIDVDGDVDGDGDGDVDVDVDP
                     ASDSDGREGWEAVLPAALAAGEAQLAVRDGRILSPRLVRTPVTQETSTSPWDTEGTVLI
                     TGGTGSLGREVARHLVVTHGVRHLVLAGRRGMEAPGARESAAELSGLGASVSVVACDVA
                     DRDAVDGLLSQLSAERPLRAVVHTAGVLDDGTVTGLTPERLAAVMRPKVDAAVNLHLAT
                     RELDLDAFVLFSSAAGVFGNPGQANYAAANTFLDALAAHRRTRGLPAVSLAWGLWDENG
                     GMAAGLQDGDRARLTRTGAQALSNEQGLALFDAATAAGQRALLVPIRLEMGVLRAQAGS
                     GTLSPLLRGLVRTPARRTAAARAQSGNRSSDFGRRLTGLAEPEQEGLLLDLVCAHVAAV
                     LGHVSSGGIDAEQAFRSLGFDSLMAVELRNRLNDATGLRLPASLVFDHPTPVVLARHLR
                     AELAGTRDVADGTRTADNRTADNRTADTRANAGSADEPLAIIGMACRYPGGVQTPEDLW
                     QMVLEGRDATTPLPDDRGWDPAAIYDPDPDAVGKNYAVAGGFLQDAAGFDADLFGISPR
                     EALTMDPQQRLLLETSWEVFERAGIAPESLSGSQVGVFAGAMGSDYMATLKVPDELEGY
                     SATGSAGSVISGRIAYTFGLEGPAVTVDTACSSSLVAMHLAGQALRGGECSMALAGGVQ
                     VIATPKGFIEFSRQRALAPDGRSKAFSAAADGTGWGEGVGMLLLERLSDPSQQRVIRAA
                     LANAGLTPNDIDAVEAHGTGTPLGDPIEAQALQATYGRDRTTDDPLWLGSIKSNIAHTQ
                     AAAGVAGVIKMVMALRNGVLPKTLHADEPSPHVDWSAGAVELLTESRDWPRTDGRPRRA
                     GVSSFGISGTNAHVIVEQAPESSVEAVGERVPVGVVPWVVSARSAEGLAGQAESVEAVG
                     ERVPVGVVPWVVSARSAEGLAGQAERLARFVEERAELDLTDVGFSLAGSRSVLEHRAVV
                     LGQDRDGLLDGLRALAEDSPSAGVVSGVGDVRGRVVFVFPGQGSQWVGMAV"
     CDS_motif       5768..5803
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS44875_0001"
                     /evalue="6.80E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="80"
                     /protein_start="68"
                     /score="16.2"
                     /tool="antismash"
                     /translation="YWVRHVREAVRF"
     aSDomain        6143..6655
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS44875_PKS_DH.1"
                     /evalue="2.20E-63"
                     /label="ASD48_RS44875_PKS_DH.1"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="364"
                     /protein_start="193"
                     /score="205.5"
                     /tool="antismash"
                     /translation="HPLLTAAVGLADGQGVVLTGRLSLRTHPWLADHAVAGTVLLPGTA
                     FVELAIRAGDEAGCGRLEELALEAPLLMTEDDALAVQVRVGAPDEAGRRPVGVYSRSGN
                     SGDEQEWARHASGVLTADTPGEALDRTEGALDLTVWPPADTEPIDLSGFYEASATSGYG
                     YGPAFQGL"
     aSModule        6143..8893
                     /domains="nrpspksdomains_ASD48_RS44875_PKS_DH.1"
                     /domains="nrpspksdomains_ASD48_RS44875_PKS_KR.1"
                     /domains="nrpspksdomains_ASD48_RS44875_PKS_PP.1"
                     /incomplete
                     /locus_tags="ASD48_RS44875"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       6227..6301
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS44875_0002"
                     /evalue="1.60E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="246"
                     /protein_start="221"
                     /score="33.4"
                     /tool="antismash"
                     /translation="WLADHAVAGTVLLPGTAFVELAIRA"
     CDS_motif       6785..6811
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS44875_0003"
                     /evalue="5.40E+01"
                     /label="PKSI-KS_m4"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="416"
                     /protein_start="407"
                     /score="-1.6"
                     /tool="antismash"
                     /translation="DERASGFGM"
     aSDomain        7808..8347
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS44875_PKS_KR.1"
                     /evalue="1.10E-63"
                     /label="ASD48_RS44875_PKS_KR.1"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="928"
                     /protein_start="748"
                     /score="206.4"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLITGGTGSLGREVARHLVVTHGVRHLVLAGRRGMEAPGARES
                     AAELSGLGASVSVVACDVADRDAVDGLLSQLSAERPLRAVVHTAGVLDDGTVTGLTPER
                     LAAVMRPKVDAAVNLHLATRELDLDAFVLFSSAAGVFGNPGQANYAAANTFLDALAAHR
                     RTRGLPAVSLAWGLWDE"
     CDS_motif       7811..7912
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS44875_0004"
                     /evalue="6.90E-11"
                     /label="PKSI-KR_m1"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="783"
                     /protein_start="749"
                     /score="34.7"
                     /tool="antismash"
                     /translation="TVLITGGTGSLGREVARHLVVTHGVRHLVLAGRR"
     CDS_motif       8189..8299
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS44875_0005"
                     /evalue="6.40E-24"
                     /label="PKSI-KR_m4"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="912"
                     /protein_start="875"
                     /score="76.6"
                     /tool="antismash"
                     /translation="LDAFVLFSSAAGVFGNPGQANYAAANTFLDALAAHRR"
     aSDomain        8636..8893
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS44875_PKS_PP.1"
                     /evalue="9.70E-33"
                     /label="ASD48_RS44875_PKS_PP.1"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="1110"
                     /protein_start="1024"
                     /score="104.4"
                     /tool="antismash"
                     /translation="LTGLAEPEQEGLLLDLVCAHVAAVLGHVSSGGIDAEQAFRSLGFD
                     SLMAVELRNRLNDATGLRLPASLVFDHPTPVVLARHLRAEL"
     aSDomain        9002..10183
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS44875_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.70E-99"
                     /label="ASD48_RS44875_PKS_KS.1"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="1540"
                     /protein_start="1146"
                     /score="325.6"
                     /tool="antismash"
                     /translation="AIIGMACRYPGGVQTPEDLWQMVLEGRDATTPLPDDRGWDPAAIY
                     DPDPDAVGKNYAVAGGFLQDAAGFDADLFGISPREALTMDPQQRLLLETSWEVFERAGI
                     APESLSGSQVGVFAGAMGSDYMATLKVPDELEGYSATGSAGSVISGRIAYTFGLEGPAV
                     TVDTACSSSLVAMHLAGQALRGGECSMALAGGVQVIATPKGFIEFSRQRALAPDGRSKA
                     FSAAADGTGWGEGVGMLLLERLSDPSQQRVIRAALANAGLTPNDIDAVEAHGTGTPLGD
                     PIEAQALQATYGRDRTTDDPLWLGSIKSNIAHTQAAAGVAGVIKMVMALRNGVLPKTLH
                     ADEPSPHVDWSAGAVELLTESRDWPRTDGRPRRAGVSSFGISGTNAHVIVEQAP"
     CDS_motif       9479..9526
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS44875_0006"
                     /evalue="8.30E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="1321"
                     /protein_start="1305"
                     /score="20.9"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       9668..9706
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS44875_0007"
                     /evalue="7.90E-02"
                     /label="PKSI-KS_m4"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="1381"
                     /protein_start="1368"
                     /score="6.7"
                     /tool="antismash"
                     /translation="FSAAADGTGWGEG"
     CDS_motif       9809..9853
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS44875_0008"
                     /evalue="4.80E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="1430"
                     /protein_start="1415"
                     /score="12.4"
                     /tool="antismash"
                     /translation="VEAHGTGTPLGDPIE"
     CDS_motif       10583..10627
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS44875_0009"
                     /evalue="1.10E-07"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="ASD48_RS44875"
                     /protein_end="1688"
                     /protein_start="1673"
                     /score="23.8"
                     /tool="antismash"
                     /translation="VFVFPGQGSQWVGMA"
ORIGIN
        1 gcggtgcagg cgggtgaggc ggaggtcgtt gccgctcttg agggtttcgc cgatcgtgtg
       61 gcggttgccg cggtcaatgg tccgtcgtcg gtggtggtct ccggtgaggc ggaggctgtg
      121 gagcaggtgg tcgcgcggct gggggaggtg aagtcccgtc gtctgcgggt cagtcacgcg
      181 ttccattcgc cgttgatgga gccgatgctg gcggacttcc gggaggtcgt cgagcaggtc
      241 acctttcagg agccaactgt ccccgtcgtc tccaatgtgt ccggtcgatt cgcggagccg
      301 ggtgagctga ccacgcccga ctactgggtt cggcatgtgc gcgaggcggt ccgtttcggt
      361 gacggtgtcg gcgcgctggc cgctgacggt gtgggtgtgc tggtggaggt cggtccggac
      421 agcgttctga ccgcgctggc ccgggagtcc ttcgacggcg gggacggtct gcgggcggtt
      481 ccgctgctgc gcaaggaccg acccgagccg gagaccctgc tgaccggggt cgcgcaggcg
      541 ttcacgcacg gtgtcgaggt cgactggtcg gccatgctgc cgggtgggcg tcccgtcgag
      601 ttgcccacct acgccttcca gcgccggcgc tactggctgg aggacgccga ctccgccggt
      661 ggtgatccgg cagccttggg gctgaccgcc gcggatcatc ctctcctcgg ggcggccgtc
      721 ctcctggcag gggaccaggg agcgctcctg accggacacc tgtcggtgcg tacccagccc
      781 tggctggcgg atcacgcggt ggccgggacg gtgctgctgc cgggcacggg tttcgtggag
      841 ctggcgatcc gggccgggga cgaggtgggc tgcggacgcc tggaggaact caccctggaa
      901 gcaccgctgg tgatacccga gggcggtggc gtcccggtgc aggttcgggt cggtcccgag
      961 gaggtacccg gccgacgccc ggtgacggtt cactcgtcgg cagccgaggg cgaggagtgg
     1021 gtgcggcacg ccggcggtgt gctcacggcc gacacggccg acacggctga cgcgtccgag
     1081 gaggcctccg gcctgacgtc ctggcccccg gccgacgccg aacccgtcga cctcgagggc
     1141 ttctacgagc gtctgagcac gtcgggctac ggatacggcc ccgccttccg gagtctccgg
     1201 gcggcctggc ggcgcggcga cgagatcttc gccgaggtga cactgcccga gcagcaggac
     1261 gtgtccgggt tcggtgttca ccccgcgctg ctcgacgccg ccctgcacgc tgtgatggcc
     1321 accggtgacg gtcacgaggg catccggctg ccgttctcct ggagcggggt gtcactgttc
     1381 gcggcgggcg cctcgaacgt gcgtgtgcgg ttggctcccg cgggacccga cgcggtgtcc
     1441 gtggaggtcg cggacgccaa ggggcagccg gtggtgtccg tggacgccct ggtgtcgcgg
     1501 gaggttcagg cggatcagct cccacgcgac cagcgcgcac gggacaagga ccagggcgga
     1561 ctgttcgcgt tggaatggac gcccgctccc gtcgtggacg ccccgcccgc ccactggacg
     1621 ccgctcggcg ccgacgaaca ccaagtggtc gagaagcagg ccgagttcgt cgtcctgcca
     1681 tggacgcccc cgacggacgc ggatgatctg cccggagcgg tccgcgaggt caccgggcag
     1741 gtgctcgaag cggtacgccg gtggctctcg gacgagtacc aggaccagca ccaggaccag
     1801 gaccaggacc agaatcaaga tcagaatcag ggccaacccc aggatggcgc ctcggccgcg
     1861 tccaagctgg tcgtcgtcac ccacggggcc gtgagcgccg gccccgacga ccccgtaccc
     1921 gacctcgcgc acgcggccgt atgggggctg gtgcgctcgg cgcagtcgga acatccgggc
     1981 cggttcgtac tggtcgacat cgatgaacgg gacgactggc agagcgtgtt gggctcggcg
     2041 ctgcgccggg acgagccgca gatggtcgtg cgcggcggca ccgtgctgac accccgcctg
     2101 gtcggcgtcg cacccgagga ggacccgaac tcggcgaagg agtggaattg ggatcccgac
     2161 ggaaccgtgg tgatcaccgg cggtacgggg gccctcggcc ggctgctcgc tcggaggctg
     2221 gtgaaggatc gcggcatgcg acggctggtg ctcgccagcc ggagcggtcc cgaggccgac
     2281 agcgcgacgg aattgcggga ggaactggag agtgagggtg cccgcgtctc ggtcgtcgcc
     2341 tgcgacgtga ccgacccgga cgccgtggcc gaactgatcg cgaaggtccc ggaagagcat
     2401 ccgctgacgg ccgtcgtgca cacggcgggt gtcctggacg acggtgtgat cgagacgctc
     2461 acgcgcgacc ggctcgacgt cgtactgcga gcgaagaccg acgccgcggt gcacctgcac
     2521 cgggcgacgc gtgatctccc gctcgtcgac ctcgtcctgt tctcgtccgt cgccggcgtc
     2581 ctgggcgccc ccgggcaggc caactacgca gccgcgaaca ccttcctgga cgcgctcgcc
     2641 cagcaccgca gggcgcaggg tctgccggcg gtgtccctgg cctgggggct gtgggcgcag
     2701 gacggcggca tggccgggga gctggcgggc gagaacctgg agcgcgtcgc ccgcaccggc
     2761 ctggatccgc tcacccccga gcaaggcctc gacctcttcg agacggtcgt ccgtacgaac
     2821 cgtgccacgg gccgggcact cgccatcccg ctgcgcctga acacgggcgt gctgcgagcg
     2881 cgcggagact ccgccgggct gccggtcctg ctgcggggcc tggtgagcgg cccggtcagg
     2941 cgtacggcgc actccaccgg cgcgaccggt gggatcggca cggaggtgtc cctcgcacac
     3001 cggctggaag gcatcccgga gagcgagcag gagcgcgtcg tactggatct cgtacgcgag
     3061 cacgtggcct cggtgctggg gcacccctcg gtgaccggca tcaacccgga gcaggcgttc
     3121 caggacctcg gcttcgactc gctcaccgcg gtcgaactgc gcaaccggct cggcgcggcc
     3181 accggcctgc ggctccccgc gaccgcggtg ttcgaccatc cgtcaccggc cgcgctgggc
     3241 cggtacgtgc gggcgcgggt cgcccccgca cccgccgacc ccacgacccg gctcctcacc
     3301 gacctcgacc ggttcgaggc ggaactgctc gggacgaccc cggacgacga cgactccgta
     3361 cgcatcacga accgcctgcg cgcggtgctc tcgaagtgga ccgagcagcg ccgacgcgtg
     3421 gacgcgagcg accaggacca gacggaccgg agcgcggtgg gcgaccacct cgcgacggcg
     3481 accgccgaag agatcttcga cttcatcgac cgcgggctcg gccgcaagaa gggcgacagc
     3541 tagagatggc cagtgacgag aagcttgtcg agtacctcaa gtgggtcacg gccgatctgc
     3601 acgagacccg cgaacggctg caggaactcg agtccggcag ccgggaaccc atcgcgatca
     3661 tcggcatgag ctgccgctac cccggtgacg tgcggtcccc cgaggagctg tgggagctcg
     3721 tgcggaccgg taccgacgcg gtgtcggact tcccaggcaa ccgcggctgg gacgtcgagg
     3781 ggctctacga cccggatccc gagcggcgcg gaaagctcta cgcacgcgag ggcggattcc
     3841 tgcacgacgc ggaccgcttc gacgccggct tcttcggcat cagcccgcgc gaggcgaccg
     3901 cgatcgaccc gcagcaacgg ctgctgctgg agacctcctg ggaggccttc gaacgggccg
     3961 ggatcgcacc cgactccctg cgcggcaccc ggaccggcgt tttcacggga gtcatgtacg
     4021 acgactacgg cgtacgcatg cggcccatgc ccgaggcctt cgagggcctc ctcggcagcg
     4081 gcagcgcggg cagcgtggcc tccggtcggg tcgcctacac cttcggcctc gaggggcccg
     4141 cggtgacggt ggacacggcg tgctcgtcgt ccctggtggc gatgcacctc gcgagccagg
     4201 cactgcggga cggtgagtgc tcgctggcgc tcgcgggcgg cgtcgcgctg atggccaccc
     4261 ccacgatctt catcgagttc agccgtcagc ggggactggc ccgcgacggc cgctgcaagc
     4321 ccttctccga cgcggccgac ggcaccggct ggggcgaagg cgtcggtgtc ctcctgctgg
     4381 agcgcctgtc ggacgcccgc cgcaacgggc atcggatcct cgcggtcctg cggggcagcg
     4441 cggtgaacca ggacggcgcg agcaacggcc tcacggcacc caacggcccg tcccagcaac
     4501 gcgtcatcga gactgccctg gccaacgcgc agctgtcgac gaacgacgtc gacgccgtgg
     4561 aggcccatgg cacgggcacc tccctcgggg accccatcga ggcgcaggcg ctgctggcca
     4621 cctatggcca ggggcgtccg gaagagcggc cgttgtggct cggctcggtc aagtcgaaca
     4681 tcgcgcacac gcaggccgcg gcgggtgttg ccggcgtcat caagatggtg atggccctgc
     4741 ggcacggggt gctgccgaag accctgcatg tggacgctcc ctcggcgcac gtggactggg
     4801 ctgcgggcgc ggtggagttg ctgaccgaat cccgggactg gccgcggacc gacgggcgtc
     4861 cgcggcgggc cggtgtgtcg tcgttcggga tcagcgggac gaacgcgcac gtgatcgtgg
     4921 aagaggcgcc cgagtcctct gccgacgcgg ttgccgagtc gggtgtccgt gtcccggttc
     4981 cggtggtgcc gtgggtggtg tcggcgcgcg gggccgacgg gctggccgcc caggccgagc
     5041 ggttggccgg gttcgtggaa gagcgggccg agctggaccc ggtggatgtg gggttctcgc
     5101 tggcagcctc acgagcggta ctggagcgcc gggccgtggt gatgggtcag gaccgggatg
     5161 ggctgcgcga cgccctgcgg gccctggccg agggctcgcc gagtgcgggt gtggtgtccg
     5221 gggtggctcg gggacgggcg cgggtggcgt tcgggttctc ggggcagggg gcgcagcggg
     5281 tcgggatggg tgccgaactt gcctccgtct atccggtgtt cgcggaggct ttggcggagg
     5341 tctcgggtgc cttgggtctg gatcccgagg tgttcgggga tgtggatcgg ttggggnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnngtccg tcgtcggtgg tggtctccgg tgaggcggag gctgtggagg aagtcgtcgc
     5581 gcggctgggg aaggtcaagt ccaagcgtct gcgggtcagt cacgcgttcc actcgccctt
     5641 gatggaaccg atgctggctg acttccggca ggtcgccgag cagatcactt acaacgagcc
     5701 gcagctgccg gtggtctcga acgtgtccgg ccgacttgcc gagccgggtg aactgaccac
     5761 acccgactac tgggtgcggc atgtgcgtga ggcggtccgt ttcggtgacg gtgtcggcgc
     5821 gctggccgct gacggtgtcg gtgtgctggt ggaggtgggt ccggacagtg tcctgaccgc
     5881 gctggcccgg gagtccttcg acggcgggga cggtatgcgg gcggttccgc tgctgcgcaa
     5941 ggaccgtccc gagccggaga ccctgctgac cggggtggcg caggcgttca cgcacggtgt
     6001 cgaggtcgac tggtcggcac tcctccccgg cggccgccgt gtggaactgc ccacctacgc
     6061 cttccagcag caaagttact ggctggagga accgtcacgg tccgcgggcg acccggccgc
     6121 gttcgggctg ggcgccgcgg atcatccgtt gctgaccgcc gcggtggggt tggcggacgg
     6181 acagggcgtg gttctcaccg gccgtctgtc actgcgtacg catccgtggt tggcggacca
     6241 tgcggtggcc gggacggtgc tgctgccggg cacggccttc gtggagctgg cgatccgggc
     6301 cggggacgag gcgggctgcg ggcgtctgga agaactcgca ctcgaagcac cgcttttgat
     6361 gaccgaggac gacgcgctcg ccgtgcaggt ccgggtcggg gcgcccgacg aggccgggcg
     6421 gcgccctgtg ggcgtgtact cccggagcgg gaactcgggc gacgagcagg aatgggcgcg
     6481 gcatgccagt ggtgtgctca ccgccgacac gccgggggag gcgctggaca ggacggaggg
     6541 ggcgctcgac ctgacggtct ggcctccggc cgacaccgaa cccatcgacc tgagcggctt
     6601 ctacgaggcg tcggccacga gcggatacgg gtacggtccc gccttccagg gcctgcgcgc
     6661 ggcttggcga cgcggcgacg agatcttcgc cgaggtgagc ctgcccgagc acgcggagca
     6721 ggcggagcag ggagagcggc cggagcaggg agagctgccg gggcggtccg gccagacgga
     6781 ccaggacgag cgcgcgagcg ggttcgggat gcatccggct ctgctggacg cggttctcca
     6841 cgcggtgatc gccgcagagt cgcggggtgc ccggccgcgg gaccccgagc cgcagggtga
     6901 cgtgcgactg cccttctcct ggaacggagt ctcgctgcac gccgccggcg ccacgaccgt
     6961 gcgggcccgc atccggctct ccggcacgga ttccgtctcc ctccaggtgg cggacggcac
     7021 gggccggccc gtgatgtcgg tcgacgcgct ggtgtcccgc cccgtcccgg ccgaacaact
     7081 gcggcatccg gccagtggtg acgcgagcgg cctgttcgac gtcgagtggg tccccgtacc
     7141 cgccgtaccc cggacaccgg agcccgagcg gacgtgggcg gtgctcggac gcgacggcct
     7201 cgggctggac gatctcgcgc aggacgcgga caccttcccg gccttcgtcg tgttcccctg
     7261 cgtcacgacc gtcaccgcca ccgctaccga cgacctgacc gccaccgatg acctcgccgc
     7321 cgccaccgac ggcccggccg ccaccgacga ctccaccgtc atcgacggcc tggccgtggc
     7381 cgcacgtgcg gccacggtgc aagcgctgga gctcgtccgt cgctggctgt ccgatgagcg
     7441 gtacgcggac tcgcagctgg tcgtcgtgac cagcggagcc gtcgccacgt cgagtgacgc
     7501 acccgtggca gacctggtgc acgcgccggt ctgggggctg gtgcggtccg cgcaggccga
     7561 gcaccccggc cggttcgtcc tgatcgacgt cgatggcgac gtcgatggcg atggcgatgg
     7621 cgatgtcgat gtcgatgtcg atccggcttc ggattcggac ggtcgtgaag gctgggaagc
     7681 ggtcctgccc gccgctctgg cggccggcga agcccagctg gcggtgcgcg acggtcggat
     7741 cctgagcccc cgcctggtca ggactcccgt cacacaggag acgtccacga gcccgtggga
     7801 taccgagggc acggtgttga tcaccggtgg cacgggctcg ctggggcggg aggtggcgcg
     7861 gcatctggtg gtcacgcacg gggtgcggca tctggtgctc gccggtcgtc gcggcatgga
     7921 ggccccgggg gcccgggagt cggcggcgga gctgtcgggt ctgggcgcgt cggtgtcggt
     7981 ggtggcctgt gacgtggccg accgtgacgc ggtggacggg ctgctgtccc agctctcggc
     8041 ggagcgcccg ttgcgggcgg tggtccacac ggcgggcgtg ctggacgacg gtacggtgac
     8101 cgggctgacc cccgagcggc tcgcagcggt catgcggccg aaggtggacg ccgcggtcaa
     8161 cctccacctc gcgacgaggg agttggacct cgacgccttc gtcctgttct cctcggccgc
     8221 cggtgtgttc ggcaaccccg ggcaggccaa ctacgcggct gccaacacct tcctggacgc
     8281 gcttgccgcg caccgcagga cgcgtggtct gcccgccgtg tccctggcct ggggtctgtg
     8341 ggacgagaac ggcggaatgg ccgcgggcct ccaggacggc gatcgggcac ggctgacccg
     8401 tacgggcgcg caggccctgt cgaacgagca ggggctggcg ttgttcgacg ccgccaccgc
     8461 tgccgggcag cgggcgctgc tggtgccgat ccgtctggag atgggtgtcc tgcgtgccca
     8521 ggccggttcg gggacgctgt cgcccctgct gcgcggcttg gtgaggaccc cggcgcgccg
     8581 gacagccgcc gcccgggccc aatcgggcaa ccggtcgtcg gacttcggcc gacgactgac
     8641 cggcctcgcc gaaccggaac aggaaggcct gctgctggac ctcgtgtgtg cgcacgtggc
     8701 cgccgtcctc ggtcacgtct cctccggggg catcgacgcg gagcaggcgt tccggagcct
     8761 gggcttcgat tccctgatgg cggtcgagct gcgcaaccgg ctcaacgacg ccacagggct
     8821 acggctgccc gcctccctcg tcttcgacca ccccaccccc gtggtgctgg cacgccacct
     8881 ccgagcggaa ctcgcgggca cgcgggacgt ggcggacggc acccgcacgg cagacaaccg
     8941 cacggcagac aaccgcacgg cagacacccg cgcgaacgcc ggcagcgccg acgaaccgct
     9001 ggcgatcatc ggtatggcct gccgctaccc gggcggtgtg cagactccgg aagacctctg
     9061 gcagatggtg ctggagggac gcgacgcgac caccccgctc cccgacgacc gcggctggga
     9121 cccggcagcg atctatgacc cggaccccga cgccgtcggc aagaactacg cggtcgcggg
     9181 aggcttcctc caggacgccg cgggcttcga cgccgacctg ttcgggatct cgccgcgcga
     9241 ggcactgacc atggatccgc agcagcgtct gctgctggag acgtcctggg aggtgttcga
     9301 gcgggccggc atcgcacccg agtcgctgag cggcagccag gtcggtgtgt tcgcgggcgc
     9361 gatgggctcg gactacatgg ccacgctcaa ggtgccggac gagctggagg gctactcggc
     9421 caccggcagc gcgggcagcg tgatctcggg ccggatcgcc tacaccttcg gcctcgaagg
     9481 gccggcggtg accgtcgaca cggcctgctc gtcgtccctg gtggcgatgc atctcgccgg
     9541 gcaggccctg cgcggcggcg agtgctcgat ggcgctcgcg ggcggcgtcc aggtcatcgc
     9601 cactcccaag ggcttcatcg agttcagccg ccagcgtgcg ctggcgcccg acggccggtc
     9661 gaaggcgttc tcggcggcgg ccgacggtac gggctggggt gagggtgtgg ggatgctgct
     9721 gttggagcgg ctgtcggatc cgtcccagca gcgtgtcatc cgggccgcgt tggccaacgc
     9781 cggcctgacg ccgaacgaca tcgacgccgt cgaggcgcac ggcaccggca ccccgctcgg
     9841 cgaccccatc gaggcgcagg cgctgcaagc cacgtacggc cgcgaccgca ccaccgacga
     9901 tccgctgtgg ctgggatcca tcaagtccaa catcgcgcac acgcaggccg cggccggtgt
     9961 cgccggtgtg atcaagatgg tgatggcgct gcgcaacggg gtgctgccga agacactgca
    10021 cgcggacgag ccgtccccgc acgtcgactg gtccgcgggt gcggtggagt tgctgaccga
    10081 atcccgggac tggccgcgga ccgacgggcg tccgcggcgg gccggcgtgt cgtccttcgg
    10141 gatcagcggg acgaacgcgc acgtgatcgt ggagcaggcg cccgagtcgt ccgtcgaggc
    10201 ggtgggtgag cgggttccgg tcggggtggt gccgtgggtg gtgtcggcgc ggagcgctga
    10261 gggactggcc ggccaggcgg agtccgtcga ggcggtgggt gagcgggttc cggtcggggt
    10321 ggtgccgtgg gtggtgtcgg cgcggagcgc tgagggactg gccggccagg cggagcggct
    10381 cgctcggttc gtggaggagc gggccgagct ggacctgacc gatgtcgggt tctcactggc
    10441 cgggtcgcgg tcggtgctgg agcaccgggc ggtggtcctg ggacaggacc gcgacggcct
    10501 gctcgacggg ctgcgcgccc tggccgagga ctcgccgagt gcgggtgtgg tgtcgggtgt
    10561 gggtgatgtg cggggtcggg tggtgtttgt tttccctggt caggggtcgc agtgggtggg
    10621 tatggcggtg ga
//
