LOCUS       NZ_LMEQ01000022         5491 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root1310 contig_29, whole genome shotgun sequence.
ACCESSION   NZ_LMEQ01000022
VERSION     NZ_LMEQ01000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root1310
  ORGANISM  Streptomyces sp. Root1310
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140604
            Assembly Name                     :: Root1310
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426425.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:14:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,470
            CDSs (total)                      :: 8,385
            Genes (coding)                    :: 8,123
            CDSs (with protein)               :: 8,123
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 98 of 262
            Pseudo Genes (incomplete)         :: 219 of 262
            Pseudo Genes (internal stop)      :: 20 of 262
            Pseudo Genes (multiple problems)  :: 64 of 262
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:32:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5491
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEQ01000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5491
                     /collection_date="2012"
                     /country="Germany: Cologne"
                     /db_xref="taxon:1736452"
                     /host="Arabidopsis thaliana"
                     /isolation_source="root"
                     /lat_lon="50.958 N 6.856 E"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. Root1310"
                     /strain="Root1310"
                     /submitter_seqid="contig_29"
     region          1..5491
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..5491
                     /SMILES="C=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5491
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[140:>5491](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      141..>5491
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            141..>5491
                     /locus_tag="ASD48_RS24150"
                     /old_locus_tag="ASD48_41145"
     CDS             141..>5491
                     /NRPS_PKS="Domain: PKS_AT (119-404). E-value: 1.6e-100.
                     Score: 328.2. Matches aSDomain:
                     nrpspksdomains_ASD48_RS24150_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (471-635). E-value: 3.7e-68.
                     Score: 221.0. Matches aSDomain:
                     nrpspksdomains_ASD48_RS24150_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (951-1130). E-value: 4.9e-64.
                     Score: 207.6. Matches aSDomain:
                     nrpspksdomains_ASD48_RS24150_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (1225-1310). E-value: 4.7e-32.
                     Score: 102.2. Matches aSDomain:
                     nrpspksdomains_ASD48_RS24150_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (1333-1758). E-value:
                     1.8e-184. Score: 605.5. Matches aSDomain:
                     nrpspksdomains_ASD48_RS24150_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="ASD48_RS24150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41145"
                     /product="type I polyketide synthase"
                     /protein_id="WP_348983227.1"
                     /sec_met_domain="PKS_AT (E-value: 2.7e-102, bitscore:
                     328.2, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 3.5e-68, bitscore:
                     215.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.9e-17, bitscore:
                     47.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 1.1e-233, bitscore:
                     761.7, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.5e-90, bitscore:
                     289.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSFGISGTNAHVIVEQAPESAVGAVAESGVRVPVPVVPWVVSAR
                     SAEGLAAQAERLARFVGERPALDPVDVGFSLVGSRSVWEHRAVVLGRDRDALLGGLREL
                     PVRGVAAEGVRVAFAFSGQGAQRAGMGAELASXYPVFAEALAEVSGALGLDPEVFGDVD
                     RLGRTEVTQAALFAFEVAVVRLLESFGVRPDVLIGHSIGEIAAAYVAGVFSLXDAAXLV
                     GARGRLMQALPAGGVMVAVQAGEAEVVAALEGFXDRVAVAAVNGPSSVVVSGEAEAVEX
                     VVARLGEVKSRRLRVSHAFHSPLMEPMLADFRZVVEQVTFQEPTVPVVSNVSGRFAEPG
                     ELTXPDYWVRHVREAVRFGDGVRALAGEGVGVLVEVGPDSVLTALARESFDGGDGLRAV
                     PLLRKDRPEPETLLTGVAQAFTHGVQVDWPALLPGGRRVELPTYAFQRRRYWLEEHSSW
                     VGDADALGLASTEHPLLGAAVRLADGQGVVLTGRVSLRTHPWLADHAVAGTVLLPGTGF
                     VELAIRAGDEVGCGRLEELTLEAPLVVPDRTGVSVQVRVGPEEASGRRQLTVHSLAAEG
                     EEWVRHASGALTADSADTPAQTFDLTAWPPADAGPVELDGFYEALASSGYTYGPAFQGL
                     RAAWRRGDEIFAEVGLPEQEIDGASAFGIHPALLDAALHATVATDGEDDGGQVRLPFAW
                     TGVSLFATGATTVRVRLAYTGADTASLQLADPEGRPVASVDALVSRPVSKEQLRQSGGG
                     EQDALYGVDWLPAATPAAPPAATATPRYDVLVCTSASQLVNGDVPELVRETTADVLRAL
                     QGALADESPEPKRLIVVTRGAVSTGADDALRDPAHASVWGLVRSAQAEHPGRFVLVDVD
                     VDVDVDVHEHVHEHVHEDEREGEDGGGGEGHGWGALLPAVLATGEPQIAVRQGALLAPR
                     LVRTSAVVPDAGLENRAGDPDGTVLITGGTGSLGRVLARHLVVEQGMRRLVLAGRRGIE
                     APGAAELVAELAQLGASVSAVACDVTDRDALDALLAGIPAEHPLTAVVHLAGVLDDGTI
                     TALTPERLAAVLRPKVDAAVNLHLATRESDLRAFVLFSSVAGVFGGPGQGNYAAANAFL
                     DALAQHRRAAGLPAVSLAWGLWEQDGGMTGRLEEGDRARIARSGLAPLSSAQGMALFDA
                     APGRNRALLAPVRLDMAALRAQAASGTLPTLLRDLVRVPTRRTATTTEAGGPATALLRR
                     LAGLTEPEQERALLDLVRTHAAGALGHPKPDAVGPQRGFLDMGFDSLMAVELRNRLNAA
                     VDLRLPSTLIFDHPTPLALARYLRTEALGGRRTAGETLATTSVAADEPLAIIGMACRLP
                     GDIDSPEALWRLVEEGRDVVADFPLDRGWKIGDLYDPDPDAPGKSYAREGAFLYDVADF
                     DAELFGISPREALAMDPQQRLLLEASWEAFERAGLDVGGLRGSQVGVFAGAMPPDYVSR
                     LNKVPEGVEGYTMTGSTSSVISGRVSYAFGLEGPAVTVDTACSSSLVALHLAGQALRGG
                     ECTLALAGGVTVMSTPQEFVELSRQRGLARDGRCKSFSAAADGVGWGEGVAVLVLERLS
                     DARRNGHEVLAVIRGSAVNQDGASSGLTAPNGPSQQRVIRAALANAGLSPSDVDVVEAH
                     GTGTPLGDPIEAQALLATYGQERPESRPLWLGSLKSNIAHTQAAAGVAGVIKMVQAVRQ
                     GVLPKTLHVDAPSPHIDWASGAVELLTEAREWPEVDRPRRAGVSSFGISGTNAHVIVEQ
                     APESAVGAVAESGVRVPVPVVPWVVSAR"
     CDS_motif       492..542
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS24150_0001"
                     /evalue="1.00E-04"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="134"
                     /protein_start="117"
                     /score="14.9"
                     /tool="antismash"
                     /translation="FAFSGQGAQRAGMGAEL"
     aSDomain        498..1352
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS24150_PKS_AT.1"
                     /evalue="1.60E-100"
                     /label="ASD48_RS24150_PKS_AT.1"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="404"
                     /protein_start="119"
                     /score="328.2"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="FSGQGAQRAGMGAELASXYPVFAEALAEVSGALGLDPEVFGDVDR
                     LGRTEVTQAALFAFEVAVVRLLESFGVRPDVLIGHSIGEIAAAYVAGVFSLXDAAXLVG
                     ARGRLMQALPAGGVMVAVQAGEAEVVAALEGFXDRVAVAAVNGPSSVVVSGEAEAVEXV
                     VARLGEVKSRRLRVSHAFHSPLMEPMLADFRZVVEQVTFQEPTVPVVSNVSGRFAEPGE
                     LTXPDYWVRHVREAVRFGDGVRALAGEGVGVLVEVGPDSVLTALARESFDGGDGLRAVP
                     LLRK"
     aSModule        498..4070
                     /complete
                     /domains="nrpspksdomains_ASD48_RS24150_PKS_AT.1"
                     /domains="nrpspksdomains_ASD48_RS24150_PKS_DH.1"
                     /domains="nrpspksdomains_ASD48_RS24150_PKS_KR.1"
                     /domains="nrpspksdomains_ASD48_RS24150_PKS_PP.1"
                     /locus_tags="ASD48_RS24150"
                     /monomer_pairings="mal -> ccmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     CDS_motif       708..830
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS24150_0002"
                     /evalue="2.00E-22"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="230"
                     /protein_start="189"
                     /score="71.5"
                     /tool="antismash"
                     /translation="GVRPDVLIGHSIGEIAAAYVAGVFSLXDAAXLVGARGRLMQ"
     CDS_motif       924..962
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS24150_0003"
                     /evalue="4.80E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="274"
                     /protein_start="261"
                     /score="13.4"
                     /tool="antismash"
                     /translation="AAVNGPSSVVVSG"
     CDS_motif       1179..1214
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS24150_0004"
                     /evalue="7.30E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="358"
                     /protein_start="346"
                     /score="16.1"
                     /tool="antismash"
                     /translation="YWVRHVREAVRF"
     aSDomain        1554..2045
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS24150_PKS_DH.1"
                     /evalue="3.70E-68"
                     /label="ASD48_RS24150_PKS_DH.1"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="635"
                     /protein_start="471"
                     /score="221.0"
                     /tool="antismash"
                     /translation="HPLLGAAVRLADGQGVVLTGRVSLRTHPWLADHAVAGTVLLPGTG
                     FVELAIRAGDEVGCGRLEELTLEAPLVVPDRTGVSVQVRVGPEEASGRRQLTVHSLAAE
                     GEEWVRHASGALTADSADTPAQTFDLTAWPPADAGPVELDGFYEALASSGYTYGPAFQG
                     L"
     CDS_motif       1638..1712
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS24150_0005"
                     /evalue="1.30E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="524"
                     /protein_start="499"
                     /score="33.7"
                     /tool="antismash"
                     /translation="WLADHAVAGTVLLPGTGFVELAIRA"
     aSDomain        2994..3530
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS24150_PKS_KR.1"
                     /evalue="4.90E-64"
                     /label="ASD48_RS24150_PKS_KR.1"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="1130"
                     /protein_start="951"
                     /score="207.6"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLITGGTGSLGRVLARHLVVEQGMRRLVLAGRRGIEAPGAAEL
                     VAELAQLGASVSAVACDVTDRDALDALLAGIPAEHPLTAVVHLAGVLDDGTITALTPER
                     LAAVLRPKVDAAVNLHLATRESDLRAFVLFSSVAGVFGGPGQGNYAAANAFLDALAQHR
                     RAAGLPAVSLAWGLWE"
     CDS_motif       2997..3098
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS24150_0006"
                     /evalue="5.30E-10"
                     /label="PKSI-KR_m1"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="986"
                     /protein_start="952"
                     /score="31.9"
                     /tool="antismash"
                     /translation="TVLITGGTGSLGRVLARHLVVEQGMRRLVLAGRR"
     CDS_motif       3375..3485
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS24150_0007"
                     /evalue="1.10E-22"
                     /label="PKSI-KR_m4"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="1115"
                     /protein_start="1078"
                     /score="72.6"
                     /tool="antismash"
                     /translation="LRAFVLFSSVAGVFGGPGQGNYAAANAFLDALAQHRR"
     aSDomain        3816..4070
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS24150_PKS_PP.1"
                     /evalue="4.70E-32"
                     /label="ASD48_RS24150_PKS_PP.1"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="1310"
                     /protein_start="1225"
                     /score="102.2"
                     /tool="antismash"
                     /translation="LAGLTEPEQERALLDLVRTHAAGALGHPKPDAVGPQRGFLDMGFD
                     SLMAVELRNRLNAAVDLRLPSTLIFDHPTPLALARYLRTE"
     aSDomain        4140..5414
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS24150_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.80E-184"
                     /label="ASD48_RS24150_PKS_KS.1"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="1758"
                     /protein_start="1333"
                     /score="605.5"
                     /tool="antismash"
                     /translation="AIIGMACRLPGDIDSPEALWRLVEEGRDVVADFPLDRGWKIGDLY
                     DPDPDAPGKSYAREGAFLYDVADFDAELFGISPREALAMDPQQRLLLEASWEAFERAGL
                     DVGGLRGSQVGVFAGAMPPDYVSRLNKVPEGVEGYTMTGSTSSVISGRVSYAFGLEGPA
                     VTVDTACSSSLVALHLAGQALRGGECTLALAGGVTVMSTPQEFVELSRQRGLARDGRCK
                     SFSAAADGVGWGEGVAVLVLERLSDARRNGHEVLAVIRGSAVNQDGASSGLTAPNGPSQ
                     QRVIRAALANAGLSPSDVDVVEAHGTGTPLGDPIEAQALLATYGQERPESRPLWLGSLK
                     SNIAHTQAAAGVAGVIKMVQAVRQGVLPKTLHVDAPSPHIDWASGAVELLTEAREWPEV
                     DRPRRAGVSSFGISGTNAHVIVEQAP"
     CDS_motif       4620..4667
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS24150_0008"
                     /evalue="8.80E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="1509"
                     /protein_start="1493"
                     /score="20.8"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       5043..5087
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASD48_RS24150_0009"
                     /evalue="4.00E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="ASD48_RS24150"
                     /protein_end="1649"
                     /protein_start="1634"
                     /score="12.6"
                     /tool="antismash"
                     /translation="VEAHGTGTPLGDPIE"
ORIGIN
        1 ccgtgagcgc ggagctatcc ccggcgggct ggtgtgtcgt cgttcgggat cagcgggacg
       61 aacgcgcacg tgatcgtgga gcaggcgccc gagtccgctg tcggggcggt tgccgagtcg
      121 ggtgtccccg gcgggctggt gtgtcgtcgt tcgggatcag cgggacgaac gcgcacgtga
      181 tcgtggagca ggcgcccgag tccgctgtcg gggcggttgc cgagtcgggt gtccgtgtcc
      241 cggttccggt ggtgccgtgg gtggtgtcgg cgcgcagtgc ggaagggctg gccgcccagg
      301 ccgagcggct cgcccgattc gtcggcgagc gccccgcgtt ggacccggtg gatgtggggt
      361 tctcgctggt cgggtcgcgn tcggtgtggg agcaccgggc ggtggtgctg gggcgcgatc
      421 gggacgcatt gctnggtggg ttgcgggagt tgccggtgcg gggtgtggcc gctgagggcg
      481 tgcgggtggc gttcgccttc tcggggcagg gggcgcagcg ggccggaatg ggtgccgaac
      541 ttgcctccgn ctanccggtg ttcgcggagg ctttggcgga ggtctcgggt gccttgggtc
      601 tggatcccga ggtgttcggg gatgtggatc ggttgggncg gacggaggtg acgcaggctg
      661 cgctgttngc ntttgaggtg gcggtggtgc ggttgttgga gagtttcggg gtgcggcctg
      721 atgtgttgat cgggcattcg atcggggaga tcgcggcngc gtatgtggcg ggggtgttct
      781 cgctggngga tgcggcnnnn ntggtggggg cgcggggtcg gttgatgcag gcgttgccgg
      841 ctggtggcgt gatggtngcg gtgcaggcgg gtgaggcgga ggtcgttgcc gctcttgagg
      901 gtttcgncga tcgtgtggcg gttgccgcgg tcaatggtcc gtcgtcggtg gtggtctccg
      961 gtgaggcgga ggctgtggag cnggtggtcg cgcggctggg ggaggtgaag tcccgtcgtc
     1021 tgcgggtcag tcacgcgttc cattcgccgt tgatggancc gatgctggcg gacttccggn
     1081 aggtcgtcga gcaggtcacc ttncaggagc caactgtccc cgtcgtctcc aatgtgtccg
     1141 gtcgattcgc ggagccgggt gagctgacca ngcccgacta ctgggttcgg catgtgcgcg
     1201 aggcggtccg tttcggtgac ggtgtccgcg cgctggccgg tgagggcgtg ggtgtgctgg
     1261 tggaggtcgg tccggacagt gtcctgaccg cgctggcccg ggagtccttc gacggcgggg
     1321 acggtctgcg ggcggttccg ctgctgcgca aggaccgtcc cgagccggag accctgctga
     1381 ccggggtggc gcaggcgttc acgcacggcg tccaggtcga ctggcccgca ctcctccccg
     1441 gcggccgccg tgtggaactg cccacctacg ccttccagcg ccggcgctac tggctggagg
     1501 aacacagctc ctgggtgggc gatgcggacg cgctcgggct ggcgtccacc gagcatccac
     1561 tgctgggggc ggcggtgcgg ctggccgacg gacagggtgt ggtgctgacg ggtcgggtgt
     1621 cgctgcgtac acatccgtgg ttggcggacc atgcggtggc cgggacggtg ctgctgccgg
     1681 gcacgggttt cgtggagctg gcgatccggg ccggggacga ggtgggctgc ggacgcctgg
     1741 aggaactcac tctcgaagca ccgctcgtcg ttcccgacag gacgggtgtg tcggtgcagg
     1801 tccgcgtcgg gcccgaagag gcttccgggc gcaggcagtt gacggttcac tcgctggcag
     1861 ccgagggcga ggagtgggtg cggcacgcca gcggtgcgct cacggccgac tcggccgata
     1921 ctccggcgca gacgttcgac ctgacggcct ggccccccgc cgacgccggc cccgtcgaac
     1981 tcgacggctt ctacgaagcg ctcgcctcct cgggctacac ctacggcccc gcgttccagg
     2041 gccttcgtgc cgcctggcgg cgcggcgacg agatcttcgc cgaggtgggc ctgcccgagc
     2101 aggagatcga cggcgcgtcc gccttcggaa tccatccggc tctgctggac gccgctctgc
     2161 acgcgacggt ggcgaccgac ggagaggatg acggcggtca ggttcggctg ccgttcgcct
     2221 ggaccggggt ctcactgttc gccacgggtg ccacgaccgt tcgcgtacgg ctggcgtaca
     2281 ccggcgccga caccgccagc cttcagttgg cggacccgga gggccgtccg gtcgcgtccg
     2341 tcgacgctct ggtctcccgc ccggtgtcca aggaacagct gaggcagtcg ggaggcggcg
     2401 agcaggacgc gctgtacggc gtggactggt tgcccgccgc gacgcccgct gcgccgccgg
     2461 ccgcgacggc cacccccagg tacgacgtgc tggtgtgcac gtcggcgtcg cagctcgtga
     2521 acggggacgt tccggagctc gtgcgggaga ccacggccga cgtactgcgg gccctgcaag
     2581 gggcgttggc cgatgagtcg ccggagccga aacgactgat cgtggtcacc cggggcgcgg
     2641 tgagcaccgg ggcggacgac gccctgcgtg atcctgcgca cgcgtcggtg tgggggctgg
     2701 tgcgttccgc gcaggcggag caccccggcc ggttcgtcct ggtcgatgtc gatgtcgacg
     2761 tcgatgttga cgtccacgag cacgtccacg agcacgtcca cgaggacgag cgcgaaggcg
     2821 aggacggagg cggaggcgaa ggccacgggt ggggtgcact cctgcccgcc gtgttggcga
     2881 ccggcgaacc ccagatcgcg gtgcgccagg gagccctgct cgcaccgcgg ctggtgcgta
     2941 cgtcggccgt cgtccccgat gccggtctcg agaaccgtgc cggggatccc gacggcacgg
     3001 ttctgatcac cggcgggacg ggttcgctgg gtcgagtgct cgcgcggcac ctggtggtcg
     3061 agcaggggat gcgccggctc gtgctggccg gtcggcgtgg tatcgaggcg cccggcgcgg
     3121 ccgaactggt ggcagaactg gcacagttgg gggcgtcggt atcggccgtc gcctgcgacg
     3181 tgaccgatcg ggacgctctg gacgcactgc tggcagggat accggcggag catccgctga
     3241 cggccgtcgt ccacctggcg ggagtcctgg acgacgggac gatcacggcg ttgaccccgg
     3301 agcggctggc cgccgtactg cggccgaagg tcgacgcggc tgtcaacctg cacctggcca
     3361 cacgggagtc ggaccttcgt gccttcgtcc tcttctcctc cgtggcgggc gtcttcggcg
     3421 gaccggggca aggcaactac gcggccgcca acgcgttcct cgacgcgctc gcccagcacc
     3481 ggcgggcggc cggtctgcct gccgtgtccc tggcctgggg cctgtgggag caggacggcg
     3541 ggatgacggg gcgcctggag gagggcgacc gggcacgcat cgcgcggtcc ggcctcgcac
     3601 ctctgtcctc ggcacagggg atggccctgt tcgacgcggc gcccggccgg aacagggcac
     3661 tgctggcgcc cgtccgcctg gacatggccg cgctccgggc gcaggcggcc tcgggaaccc
     3721 tgccgacact gcttcgcgac ctggtacgcg tccccacccg acgcaccgcg accaccaccg
     3781 aggccggcgg gccggccacc gctctgctgc ggaggctggc cggcctcacc gaacccgagc
     3841 aggaacgcgc gctcctggac ctcgtacgca cccacgccgc cggagcgctc ggccacccga
     3901 agccggacgc ggtcggaccg cagcgcggtt ttctcgacat gggcttcgac tccctgatgg
     3961 cggtggaact ccgcaaccgg ctcaacgccg ccgtcgacct ccgcctgccc tccaccctga
     4021 tcttcgatca cccgaccccg ctcgcgctcg cccgttacct gcggaccgag gcgctcggag
     4081 gccggcggac ggcgggggag acccttgcga ccacgtccgt cgcggccgac gagcccctgg
     4141 cgatcatcgg catggcctgc cgcctgcccg gtgacatcga ctcgcccgag gcgctgtggc
     4201 ggctggtcga agaggggcgg gacgtcgtcg cggacttccc gctggaccgc ggctggaaga
     4261 tcggggacct gtacgacccc gaccccgacg cacccggcaa gagctacgcg cgggaaggcg
     4321 cgttcctcta cgacgtcgcc gacttcgacg ccgaactgtt cggcatctcg ccccgcgagg
     4381 cgctggccat ggatccgcag cagcgcctgc ttctggaggc gtcctgggag gccttcgagc
     4441 gagccggcct cgatgtgggc gggctgcgcg gaagccaggt cggcgtgttc gccggagcga
     4501 tgccaccgga ctacgtctcg cggctcaaca aggtgcccga aggcgtcgag ggctacacca
     4561 tgaccggcag cacctcgagc gtcatctccg gccgggtctc gtacgcgttc ggtctcgagg
     4621 ggcccgcggt gacggtggac acggcgtgct cgtcctcgct ggtggcactg cacctggccg
     4681 gtcaggccct gcgcggcggc gaatgcacgc tcgccctcgc gggcggcgtt acggtgatgt
     4741 ccactcccca ggagttcgtc gaactgagcc gccagcgcgg actggcccgc gacggccgct
     4801 gcaagtcctt ctccgccgcc gccgacggtg tgggctgggg cgaaggcgtc gccgtcctcg
     4861 tcctggagcg gctgtcggac gcgcggcgca acgggcacga ggtactggcg gtgatccggg
     4921 gcagcgccgt gaaccaggac ggcgcgagca gcggcctgac ggcgccgaac ggtccctccc
     4981 aacagcgcgt gatccgtgcc gcgttggcca acgccggtct gtccccgtcg gacgtcgacg
     5041 tcgtcgaggc ccatggcacc gggacgccgc tcggggatcc gatcgaggcg caggcgttgc
     5101 tggcgacata cgggcaggag cggccggaaa gccgacccct gtggctgggg tcgttgaagt
     5161 cgaacatcgc gcacacgcag gcggcggcgg gtgtcgccgg agtgatcaag atggtccagg
     5221 ccgtgcgaca gggggtgttg ccgaagacgc tgcacgtgga tgccccctcg ccacacatcg
     5281 actgggcctc cggtgccgtc gagctgttga cggaggcgcg ggagtggccg gaggtcgatc
     5341 gtccccggcg ggctggtgtg tcgtcgttcg ggatcagcgg gacgaacgcg cacgtgatcg
     5401 tggagcaggc gcccgagtcc gctgtcgggg cggttgccga gtcgggtgtc cgtgtcccgg
     5461 ttccggtggt gccgtgggtg gtgtcggcgc g
//
