LOCUS       NZ_LMEQ01000019        10993 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root1310 contig_26, whole genome shotgun sequence.
ACCESSION   NZ_LMEQ01000019
VERSION     NZ_LMEQ01000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root1310
  ORGANISM  Streptomyces sp. Root1310
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 16184)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 16184)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140604
            Assembly Name                     :: Root1310
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426425.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:14:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,470
            CDSs (total)                      :: 8,385
            Genes (coding)                    :: 8,123
            CDSs (with protein)               :: 8,123
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 98 of 262
            Pseudo Genes (incomplete)         :: 219 of 262
            Pseudo Genes (internal stop)      :: 20 of 262
            Pseudo Genes (multiple problems)  :: 64 of 262
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:32:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2441
            Orig. end                         :: 13434
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEQ01000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10993
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10993
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10993
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5993](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5993)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(355..369)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.77"
                     /tool="antismash"
     misc_feature    complement(362..375)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.65"
                     /tool="antismash"
     gene            627..956
                     /locus_tag="ASD48_RS24060"
                     /old_locus_tag="ASD48_41055"
     CDS             627..956
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASD48_RS24060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ASD48_41055"
                     /product="hypothetical protein"
                     /protein_id="WP_157880685.1"
                     /transl_table=11
                     /translation="MTEIIEVREMRMEIWESDDPDQAETPWWGMKWVPISGSDGDDHFI
                     DAGEGVWQNHLGDAVHDDQAHFLGWPSLGSWLHEVAEAMKHHDQSSWAGAVTAPKVNSS
                     GDIHW"
     gene            complement(1192..1887)
                     /locus_tag="ASD48_RS24065"
                     /old_locus_tag="ASD48_41060"
     CDS             complement(1192..1887)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640014.1"
                     /locus_tag="ASD48_RS24065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41060"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_057603786.1"
                     /transl_table=11
                     /translation="MVKQDRAIRTRQTILSAAAKVFEEHGYQAATISEILTTAGVTKGA
                     LYFHFPSKEHLAEGVLHEQDQLLPIPDRTCKIQQFVDTVMLHAHRLQTDAMVRAGVRLS
                     LDQQALGLDRSGPFLRWSQISRDLLEKAQAQGELLPHVNTVETADVMVGSFAGVQAMSQ
                     AVCDHQDLPQRVNALLRHVLPSVVMPSVLASVDLTRTRGAAVDTELRTTHHTTTHTSVT
                     QPTQPTPIH"
     misc_feature    complement(1927..1936)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.17"
                     /tool="antismash"
     gene            2166..3107
                     /locus_tag="ASD48_RS24070"
                     /old_locus_tag="ASD48_41065"
     CDS             2166..3107
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387428.1"
                     /locus_tag="ASD48_RS24070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41065"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_079073956.1"
                     /transl_table=11
                     /translation="MILVTGVTGTVGREVAGLLPAGAGVRVMARDPGRVTGVAAGVEVV
                     AGDYGDRGSLVPVLRGVRTAFLVTARVGGGDDAVFVAAARAAGVRRVVKLSAAAVLDGG
                     AGDLITRWQRESEELLRVSGMEWTLLRPRAFMSNALSWADSVRREGVVRALYGEAAHAC
                     VDPADVARVAVRVLTEEGHAGCAYTLTGPQALSAAEQTRQLGALLGVALRFEELSPGRA
                     RAALAGRFAPEVVEALLDSAQRLKAGAKAGVQDTVSRLTGRPARTFRQWAAAHVASFGP
                     VAAAAVPQGSQAVPRARGEVPRGPGGGADAAL"
     assembly_gap    3983..4008
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5001..5993)
                     /locus_tag="ASD48_RS24075"
                     /old_locus_tag="ASD48_41070"
     CDS             complement(5001..5993)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387457.1"
                     /locus_tag="ASD48_RS24075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41070"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_057603788.1"
                     /sec_met_domain="AfsA (E-value: 3.6e-28, bitscore: 87.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSASTFRMNGAISGAVRTESGPIPLAGTGAFLFRSLTTTVPKELV
                     HRASVAEVMLTDWARVDEERFTVSAQWPRGHSFFAAVDGCHDPLIAAETIRQAGSLLAH
                     AEFGVPLDHHFLMWDLAIAVEPAHLRVGDTPASVELEISCSDVKRRRGELAGLHYDAVI
                     RRGGHIAATGGATFTCTSAKVYERLRGERAASIHRPLPLTAPAAPQNVGRVSPMDVVLS
                     PIGQPDRWQLRADTAHPILFDHPVDHVPGMVLLEAARQATTAVLGHSTLPLSITSEFTK
                     YAELDAPCLIEARRLTRTPAQRGPAVLVTAHQHGTPVFRSTVTAAPLTI"
     misc_feature    6067..6080
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.68"
                     /tool="antismash"
     misc_feature    complement(6070..6084)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.3"
                     /tool="antismash"
     misc_feature    6071..6084
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.56"
                     /tool="antismash"
     gene            6326..6514
                     /locus_tag="ASD48_RS24080"
                     /old_locus_tag="ASD48_41075"
     CDS             6326..6514
                     /GO_function="GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA]"
                     /GO_function="GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569602.1"
                     /locus_tag="ASD48_RS24080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41075"
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="WP_057603790.1"
                     /transl_table=11
                     /translation="MGTEEALIRVERGRPGEAELAAVVAVLLALQAGARRESGRAPDAR
                     MRWWRGPDAYTAPGSWR"
     gene            6643..8229
                     /locus_tag="ASD48_RS24085"
                     /old_locus_tag="ASD48_41080"
     CDS             6643..8229
                     /EC_number="6.-.-.-"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432460.1"
                     /locus_tag="ASD48_RS24085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41080"
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="WP_057603796.1"
                     /transl_table=11
                     /translation="MTTAPAPAEPVPTDIRGRVAELHSIREQALAGPGERATAAQHAKG
                     KLTARERIGLLLDPGSFQEVEQLRRHRATGFGLEVKKPYTDGVITGWGTVEGRTVFVYA
                     HDFRIFGGALGEAHAAKIHKIMDMAIATGAPLVSLNDGAGARIQEGVSALAGYGGIFRR
                     NTRASGVIPQISVILGPCAGGAAYSPALTDFVFMVRETSQMFITGPDVVRAVTGEEITQ
                     NGLGGADVHAETSGVCHFAYDDEETCLAEVRYLLSLLPQNNRETPPAAPCDDPAGRRAD
                     RLLDLVPADGNRPYDMARVIEEIVDDGEYLQVHERWATNIICALARLDGRAVALVANQP
                     RSLAGVLDIEASEKAARFVQMCDAFNIPIITLLDVPGFLPGVGQEHGGIIRHGAKLLYA
                     YCNATVPRISLVLRKAYGGAYIVMDSQSIGADLTLAWPTNEIAVMGAEGAAGVIFRRQI
                     AEAEDPEAMRQKMVKEYRSELMHPYYAAERGLVDDVIDPAETRAVLVRSLAMLRSKHAD
                     LPSRKHGNPPQ"
     gene            8306..8992
                     /locus_tag="ASD48_RS24090"
                     /old_locus_tag="ASD48_41085"
     CDS             8306..8992
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133214.1"
                     /locus_tag="ASD48_RS24090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41085"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_057603791.1"
                     /transl_table=11
                     /translation="MVKQQRAARTRHALIQAAATVFAEEGFVTASLSTISRRAGVSNGA
                     LHFHFASKHLLAEAVRAEAARTVRRITDAAQARQGGSCLQTVVDATHELMGSLAHDSVV
                     RGGFELAGDTARGGETSPRTQWQHWVEDVLRRAEHSGALAQGVSGADGAHVIVAATAGF
                     EVLGGQDPSWLSRQTITGFWEVLLPRLADRRDLDALVCAGSQRPGTLPPREQPARERSA
                     QVSPDR"
     gene            9315..10169
                     /locus_tag="ASD48_RS24095"
                     /old_locus_tag="ASD48_41090"
     CDS             9315..10169
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268975.1"
                     /locus_tag="ASD48_RS24095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_41090"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_057603793.1"
                     /transl_table=11
                     /translation="MDIEILGALAVQENGVSITPTAPKPRQVLALLALHADRVVSVSDL
                     IEELWGTTPPRSARTTLQTYVLQLRELIAAALERDAHGGPQTADGTRTPRTAKDVLVTM
                     PSGYLLAAGGGTSDVRTFERLSGMGYRAMDAGDFPGAARQLREALALWTGTAFADVQTG
                     EQLDMEIKRLEENRLCALDQRIEADLRLGRHRELLAELTVLVNRYRTHESLHGQFMLAL
                     YRSGRRGEALEVYQRLRATLVRDLGLEPSAGLQRMQRSILTASPEPAPAASGTAKERLV
                     PTS"
     misc_feature    10501..10516
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
ORIGIN
        1 cctggactgc ggcttcaagg agatcggtgt gggggtggcg cagcccggca actactggac
       61 gcaggacttc ggcacggtcc gctgacgctc gcggtgccca cgcccgtcat ggcctgccgg
      121 ccatcgcgcc ggccgcggcc acggccggcg tgggcgcgac ctgccggcag gtgccgatga
      181 gcacgcagcc cgaacaagcc ccgccccgcc tgccctgcca gccctggcca cccagctgct
      241 cgcgcagctg cgtgccgacg cctgggtgcc gttggcccgg cggccgcgcc tagggtctcc
      301 cctttggtgg tttgccaggg gccgacgtgg ctgaccgcat gagccccggc ggatgatttt
      361 ctgcatgatg atgcaacctc cccgacgagg cgtagaccct gtcgtgcccc cggtcgcgac
      421 gtcctggtgc cgcatcgacg catggttgtc ccgatacgcg ccgaaggctc tggcaggact
      481 caggcccccg gcttcgtggc agtccattca agaagccgag tcacgcctgg ggttccctct
      541 tccggcagat ctccgtgaat cgcttgcggt gccacgacgg agacacgtcc ctgctgggtg
      601 tccttccctg ccggcggctg tactcggtga cggagatcat cgaggtccgc gagatgcgga
      661 tggagatatg ggaatcggac gacccggacc aggcggaaac tccctggtgg ggaatgaagt
      721 gggtccccat ctcgggaagt gacggagacg accacttcat cgatgccggg gaaggggtgt
      781 ggcagaacca tcttggtgac gccgttcacg atgaccaggc gcattttctg gggtggccca
      841 gcttgggctc atggctccac gaggtggcgg aagccatgaa gcaccacgac caatcctcct
      901 gggccggtgc ggtgaccgcc cccaaggtca acagcagcgg ggacatccac tggtgatgct
      961 gagtcttgcc tcattgatca cctacgcgcc cgttcgtggg cgtcggcgcg tgttgagcct
     1021 ggcgcgcccg cgaggccgcg cgtcccgccg cagctgggga gaggccgcag cgacgtccgg
     1081 ctcctgagta tttcggtgcg ccggtggggg tgggggagtg gcggggtgtg tggggggtgt
     1141 gggtgctggc cggggtgtgc cgggtggtgg ggttagtggg tggtggtggg gttagtggat
     1201 gggggtgggt tgggtgggtt gggtgacgct ggtgtgggtg gtggtgtggt gggttgtgcg
     1261 tagttcggtg tcgacggcgg cgccgcgggt gcgggtgagg tcgacggagg cgaggacgga
     1321 gggcatgacg acgctgggca ggacgtggcg taggagggcg ttgacgcgtt gggggaggtc
     1381 ttggtggtcg catacggctt gggacatggc ntggacgccg gcgaaggagc cgaccatgac
     1441 gtcggcggtc tcgacggtgt tgacgtgggg gaggagttcg ccttgggcct gggctttttc
     1501 gaggaggtcg cgggagatct gggaccagcg caggaagggt ccgctgcggt cgaggccgag
     1561 ggcttgctgg tcgagggaga ggcggacgcc ggcgcggacc atggcgtcgg tctgcaggcg
     1621 gtgggcgtgg agcatgacgg tgtcgacgaa ctgctggatt ttgcaggtgc ggtcggggat
     1681 gggcaggagt tggtcctgtt cgtgcaggac gccttcggcg aggtgttctt tggaggggaa
     1741 gtggaagtac agggcgcctt tggtgactcc ggcggtggtg aggatctcgc tgatggtcgc
     1801 ggcctggtag ccgtgttctt cgaagacttt ggctgctgcg gagaggatgg tctgccgggt
     1861 ccggatcgcc ctgtcctgtt tcaccatgtt cggtctcctt ctgtttcgat gagcccggag
     1921 acccattaaa aaaaccgccc agtttgtatc ttacaggtgg ttggggttca cgcacccctt
     1981 gtggctgtac gacgttcaat gagttgctgg tggtcgatcg ggccgggaag ggggaggggg
     2041 actgtggtga gcgggtcgtg cggggggcgg ggtgtgcggg ttgtggggtg gtgcgggtgt
     2101 gggtgcgggt gcgggtgtgg gtgtgggtgt gggtgtgggt gtgggtgggt ggggaggggt
     2161 gtgcggtgat tttggtgacg ggggtgacgg ggacggtcgg gcgggaggtg gcggggctgc
     2221 ttccggcggg ggcgggggtg cgggtgatgg cgagggatcc ggggagggtc acgggggtgg
     2281 cggcgggggt ggaggtggtg gccggggatt acggggatcg gggttcgctg gtgccggtgc
     2341 tgcggggggt gcgtacggcg ttcctggtga cggcgcgggt cggcgggggt gatgatgcgg
     2401 tgttcgtggc tgcggcgcgg gcggcggggg tgcggcgggt ggtgaagctt tcggcggcgg
     2461 ctgtcctgga cggcggggcg ggtgatctga tcacccggtg gcagcgggag agtgaggagc
     2521 tgttgcgggt ttcgggtatg gagtggactc tgctgcgtcc gcgggcgttc atgtcgaacg
     2581 cgctgtcctg ggcggattcg gtgcggcgtg agggtgtggt gcgtgctttg tacggggagg
     2641 cggctcatgc ctgtgtggat cctgcggatg tggcgcgggt ggcggtgcgg gtgctgaccg
     2701 aggaggggca tgcggggtgt gcctacacgc tgacggggcc gcaggcgctc agtgccgccg
     2761 agcagacgcg gcagctgggt gcgctgctcg gtgtggcgct gcggttcgag gagctgagtc
     2821 cgggtcgggc gcgggctgct ctggcggggc gtttcgcgcc ggaggtggtc gaggcgctgc
     2881 tggacagtgc gcagcggttg aaggcgggtg cgaaggccgg ggtgcaggac actgtcagca
     2941 ggctgacggg ccgtccggcg cgtacgttcc ggcagtgggc ggcggcgcat gtcgcctcct
     3001 tcggcccggt cgccgccgcg gccgtgccgc agggatcgca ggcggtgccg cgggcgcggg
     3061 gggaggtgcc gcgggggccg gggggcgggg cggacgcggc tctgtaggac cggcgcgccc
     3121 ggcgcgcccg gcgcgtgtcc gttccccggg atgccggggg tcccgtgccg cgtgccgggc
     3181 ggtccggtac gcggcaagga cccgtcgcag cccgcagccc gcagcccgca gcccgcagcc
     3241 ggcagcggca gcggcagcgg cagcgggagc gggtcacggc agggcggggc tgccggtgcc
     3301 tgctgcgcgc gtgtgcgggg gaggcggtgt gcagcgggcc gaggggtggg ggagtccggc
     3361 aagggcccgg ggcccggggc ccggggcccc gggcgctgcg gccggggtct cgtgccgtga
     3421 gcggcgccgc cgtcacggtg gagccgggcg cggccgttcc cttcggggcg gtcaccggca
     3481 gggccggggg cggccccggg ggcggcgggt caccacaggg agtacaggcc accacagggc
     3541 gcccgcccgg ctgcgcccgg cggccggggc cgcgcagggg aggccctggc gtggcgtcag
     3601 gtcgtgccgc gccgcgctga tctgcggccc ctgccctgca gtctgggtcc cgtgctctga
     3661 ggctcatgct cggtggcggt ggcggtggcg gtggcggtgg cggtggcggt ggcggtgact
     3721 gtggcggtgg ctgtggccgg tgcccgtgct ccggggtccg tgcccggcgg gcgggggatg
     3781 tcctggcgtg gtgtcagggc ggtgggcgcg tcgcgccgac ctgcggcccc tgccctgaag
     3841 gctcatccct gtggccgggc gcccgcgggg cgctggggcc cacgccctgc gggcggctgc
     3901 cgcccntgcc tcgcggcccc tgcctcgcgg cccctgcctc gcggcccctg cctcgcggcc
     3961 cctgcctcgc ggcccctgcc tcnnnnnnnn nnnnnnnnnn nnnnnnnntg cctcgcggcc
     4021 cctgcctcgc ggcccctgcc tcgcggcccc tgcctcgcgg cccctgcctc gcggcccctg
     4081 cctcgcggcc cctgcctcgt ggtccgggcc ccgcgctctg aggctcgtgc gctgtggctg
     4141 tgtctggtgc ccgtgctccg gggtccgcgc ccggcgggcg ggggatgtcc tggcgtggcg
     4201 tcaggaccgc gtgccgctcg gacccgcggc ccctgccctg aaggctcatg cctgtggccg
     4261 ggagcccgcc gggccctgcg ccctgcgggc ggaggcggtg ggtggaggcg gtgggtggag
     4321 acggccctgg cactgcgtca gggccgcctc cgcgccggcc tgtggctcct gccttgcggt
     4381 ccgggccccg tgctctgagg ctcatgcgct gtggccggtg cccgtgctcc ggggccccgc
     4441 gtccggcggg cgggggaggc ccgggcgcgg cgtcaggatc gtgccgcgcg gtgtcgcccg
     4501 ccggcccatg ccctgaaagc cccccccgcg ctccgggccc cgcgttccgg gtcccgtgct
     4561 ccgggtcccg cgttccgggc gggcggggga ggtcctggta cggcgtcagg gcccgcgccg
     4621 tgccgcctgg cgcgggcccg tgccctgtgg ccggggcccg cgctctgcgg cccgtgcctg
     4681 gcggcctacg tcccgcgttc ctgccgccgg ggtcccgcgg gccgtgccct gcggcccggc
     4741 ccggggccct gcggccctgt gccccgcggc cggggcaccg tgtcgctgcc gccagggccc
     4801 tgcggcccgt gcccgcggct ggggccccgc gtccctgccg ccggggccct gcggccctgt
     4861 gccctgcggc cggggccccg gttcccgcgg cccggttccc gcggcccgtg cctgcggccc
     4921 tgtgccccgc gcccccgtgc cccgcgcccg cgtgtcctgc ggccggccgg cctggcccgg
     4981 cccgggccgg ggccgggggt tcatatcgtg agtggtgctg ctgtcacggt ggagcggaag
     5041 acgggggtgc cgtgctggtg ggcggtgacg aggacggcgg gtccgcgctg tgcgggggtg
     5101 cgggtcaggc ggcgggcctc gatgaggcag ggggcgtcca gttccgcgta cttggtgaat
     5161 tcgctggtga tgctgagcgg gagggtgctg tggcccagga cggcggtggt ggcctggcgg
     5221 gcggcttcca gcagtaccat tccgggcacg tggtcgacgg ggtggtcgaa gaggatgggg
     5281 tgggcggtgt cggcccgcag ctgccagcgg tccggttgtc cgatggggga cagcacgacg
     5341 tccatggggg agacgcggcc gacgttttgg ggtgcggccg gggcggtcag cggcagcggg
     5401 cggtggatgc tggcggcgcg ttcgccgcgc aggcgttcgt agaccttggc ggaggtgcag
     5461 gtgaaggtgg cgcctccggt ggccgcgatg tggccgccgc ggcggatcac cgcgtcgtag
     5521 tgcaggccgg ccagctcgcc gcggcggcgc ttgacgtcgc tgcaggagat ctccagttcg
     5581 accgaggcgg gtgtgtcgcc gacgcgcagg tgcgccggct cgacggcgat cgcgaggtcc
     5641 cacatgagga agtggtggtc gagggggacg ccgaactccg cgtgggccag gagcgatccg
     5701 gcctggcgga tcgtctcggc cgcgatcagc gggtcgtggc agccgtccac ggcggcgaag
     5761 aagctgtgcc cgcgcggcca ctgggcggag acggtgaaac gctcctcgtc cacgcgcgcc
     5821 cagtcggtca gcatgacctc ggcgacgctc gcgcggtgga cgagttcctt cgggacggtc
     5881 gtggtcaggg accggaagag gaaggcgccg gttcctgcca ggggtattgg gccgctctcg
     5941 gtgcgtaccg cgccggatat cgcgccgttc atacggaacg tgctcgcaga catgggtccc
     6001 cctgagtgcc ttgacttgaa tgtgagtggc agatccctca ggacagcgaa gatacatacc
     6061 acccggttta atttctagaa ggaacgcggc actccagtcc ggtgataaat ggagcgatgt
     6121 attcccagat gtagggacga tcatgcggcg gccgcggtga ccgcctgtga ctcctgggtg
     6181 gtgcgaggtc accgcggcgg gcgggccgcc ccgcgccccg cggcccgcga ggcgccctgt
     6241 gtcccgtatc tcaagcgatc tcaaagccgt tcttgagtgg cctggcccac ggtgtctgca
     6301 ggcccgagaa agggggaccg ctcggatggg gacggaagaa gccctgatca gggtggagcg
     6361 aggccggccc ggcgaggcgg aactggccgc cgtggtcgcg gtgctgctcg ctctgcaggc
     6421 aggtgcgcgc cgcgagagcg gccgggcgcc ggacgcccgg atgcgttggt ggcgcggccc
     6481 ggacgcctat acggcgcccg gcagctggcg ctgaccgggg gaagggatcc ctccggattc
     6541 cctccccacc aatggaaacc gctcaggcgg tttgttacga tgctcgacgg acccggggcg
     6601 ggacctcctc ggccctgccc caggaaggca cctgacatgg cgatgacgac tgccccggca
     6661 ccggccgaac cggtgcccac cgacatccgc gggcgcgtgg cggaactgca cagcatccgt
     6721 gagcaggcgc tggccgggcc cggcgagcgg gcgacggcgg cgcagcacgc caagggcaag
     6781 ctgaccgccc gcgagcggat cgggctgctc ctggaccccg gttccttcca ggaggtcgag
     6841 cagctgcgcc ggcaccgggc gacgggtttc gggctggagg tgaagaagcc gtacacggac
     6901 ggtgtgatca ccggctgggg cacggtggag ggccgtaccg tcttcgtgta cgcgcacgac
     6961 ttccgcatct tcggcggcgc gctgggcgag gcccacgccg cgaagatcca caagatcatg
     7021 gacatggcca tcgctaccgg agccccgctg gtctcgctca acgacggcgc cggcgcccgc
     7081 atccaggagg gcgtctccgc gctcgccggc tacggcggca tcttccggcg caacacccgg
     7141 gcgagcggcg tcatcccgca gatctccgtc atcctgggcc cctgcgcggg cggcgccgcc
     7201 tacagccccg ccctgaccga cttcgtcttc atggtccgcg agacctcgca gatgttcatc
     7261 accggccccg acgtcgtccg ggccgtcacc ggcgaggaga tcacccagaa cggcctgggc
     7321 ggcgccgacg tgcacgccga gacctccggc gtgtgccact tcgcctacga cgacgaggag
     7381 acctgcctcg ccgaggtccg ctacctgctg tccctgctcc cgcagaacaa ccgggagacc
     7441 ccgcccgcgg cgccctgcga cgacccggcc ggccggcgcg cggaccgtct gctcgacctg
     7501 gtccccgccg acggcaaccg gccctacgac atggcccggg tcatcgagga gatcgtcgac
     7561 gacggcgagt acctccaggt ccacgagcgc tgggcgacca acatcatctg cgccctggcc
     7621 cgcctggacg gccgggccgt cgccctggtc gccaaccagc cccggtccct ggccggcgtc
     7681 ctggacatcg aggcgtcgga gaaggccgcg cgcttcgtcc agatgtgcga cgccttcaac
     7741 atcccgatca tcaccctgct cgacgtcccc ggcttcctgc cgggcgtcgg ccaggagcac
     7801 ggcggcatca tccgccacgg cgcgaaactg ctctacgcct actgcaacgc caccgtgccg
     7861 cggatctcgc tggtcctgcg caaggcctac ggcggcgcct acatcgtcat ggactcccag
     7921 tccatcggcg ccgacctgac cctggcctgg ccgaccaacg agatcgccgt gatgggcgcc
     7981 gaaggcgccg caggcgtcat cttccgccgc cagatcgccg aggccgagga ccccgaggcc
     8041 atgcggcaga agatggtcaa ggagtaccgc tccgaactca tgcaccccta ctacgccgcc
     8101 gaacgcggcc tggtcgacga cgtcatcgac cccgccgaga cccgcgcggt cctcgtccgc
     8161 tccctggcca tgctgcgcag caaacacgcc gacctgccct cacgcaaaca cggcaacccc
     8221 ccgcaatagc acgacgtcgg tgaaagccgc cggcgccggc cgcggtgccg gcccctgcgg
     8281 cctcccgttc cccaggagag cgcacatggt caaacagcag cgagcggccc gcacccggca
     8341 tgcgctgatc caggccgccg ccacggtgtt cgccgaggag ggattcgtca ccgcgtcact
     8401 gagcaccatc agccggcgcg ccggggtcag caacggcgcg ctgcacttcc acttcgccag
     8461 caagcacctg ctcgccgaag cggtccgggc cgaggccgcc cggaccgtgc gccgcatcac
     8521 cgacgcggca caggcccgcc agggcggctc ctgcctgcag acggtggtgg acgccaccca
     8581 cgagctgatg ggcagcctcg cccacgacag cgtggtgcgc ggcggcttcg aactcgccgg
     8641 cgacaccgca cgcggcgggg agacctcccc gcgcacccag tggcagcact gggtggagga
     8701 cgtcctgcgc cgggccgagc acagcggcgc cctggcgcag ggggtctccg gggcggacgg
     8761 cgcccacgtc atcgtcgccg ccaccgccgg cttcgaggtc ctcggcggcc aggacccctc
     8821 ctggctgtca cggcagacca tcaccggctt ctgggaggtg ctgctgcccc ggctcgccga
     8881 ccgccgggac ctggacgccc tggtgtgcgc cggctcgcag cggccgggca ccctcccccc
     8941 gcgcgagcag cccgcgcgcg agcggtccgc gcaggtcagc cccgaccgat aacagaccgt
     9001 gcggtctgtc aactggccct gcgcgggaac gcggatcccc ttttcctccc ctccgggtgc
     9061 tcccgcgcgg cccggaacgg gcgccccgga gccgtgagtt ccgccccggg tgggggattc
     9121 ggctcgatcc ggtgcggtat gcgaccatgg ccggggagag aaaatacagg gtgtgcggtt
     9181 tgatattgac agaccgtgcg tgctgttctt agccttggcg gggcgccgga ccggcatctg
     9241 tcgacggcca cacaggcatc tgttgacggc cacccaatga ccggagcact cgggcacaca
     9301 acaggggaga tcgagtggac atcgagatac tcggcgcatt ggccgtacag gagaacgggg
     9361 tctccatcac gccgacggcc cccaagcccc gccaggtcct ggccctgctg gcactgcacg
     9421 ccgaccgggt ggtgtccgtc tccgacctga tcgaggagct ctggggcacc accccgccgc
     9481 gcagcgcccg caccaccttg cagacctacg tcctgcagct gcgcgaactc atcgccgcgg
     9541 ccctggagcg ggacgcgcac ggcgggccgc agacggccga cggcacccgc acgccgcgca
     9601 ccgccaagga cgtactggtc accatgccca gcggctacct gctggccgcc ggcggcggca
     9661 cgagcgacgt ccgcaccttc gagcgcctgt cgggcatggg ctaccgggcc atggacgccg
     9721 gcgacttccc cggcgccgca cggcagctgc gcgaggcact cgccctgtgg accggcaccg
     9781 ccttcgcgga cgtccagacc ggcgagcagc tggacatgga gatcaagcgc ctggaggaga
     9841 accggctgtg cgcactcgac cagcgcatcg aggccgacct gcggctggga cgccaccgcg
     9901 aactgctggc cgaactgacc gtcctggtca accgctaccg cacacacgag agcctgcacg
     9961 gccagttcat gctcgccctg taccgctcgg gacggcgcgg cgaggccctg gaggtctacc
    10021 agcggctgcg cgccacgctc gtacgcgacc tcggcctgga gccgtccgcg gggctgcagc
    10081 gcatgcagcg ctccatcctc acggccagcc ccgagcccgc accggcggca tccggcaccg
    10141 ccaaggagcg cctggtcccc accagctgag ccggcccacc gcttcccggg gtacccgccc
    10201 ggccgggtga cgcccggccc gccggcaccc cgggaagcgg caggccccag gaacggcagc
    10261 cggcggcccc cacgccgccc ccgcccccgc ccccggcggt gtcccggcgg tgtcccgcgc
    10321 ggtggacgga cgaaccgctc gcagcagcag cagcagcagc ggacggcggc cggtccggga
    10381 cggccgggga acgcggggac cgcgcccgcg cccgcggccg ggaccggatg ccggcagccc
    10441 cgggggcggg gcaccgcccg cccggcgacc cggcggccag cgccccgacc gccgcgggcc
    10501 ggcacgacgc cgtcacgggc cacggcccac cccggcccgc ccaccccggc ccgcgccccg
    10561 cccccccggt ggctgtgcag acccgcgcgg tgcaccctcc tgtgcaggct cgcgcggtgc
    10621 gcaggtcctg tccgcgtacc ggcgccggga cgcgcaacac cggccgctcg aggccgtgtc
    10681 caatgcactg ccccccggag aggtgtccgc gtccgacgtg cggggacacc cccggaaccc
    10741 ggcctgcccc ggccgccccc gccgccccgg acgcccgtgc ggctctgccc cgtgcgcctt
    10801 accccccttc cttttccttt ctccccggca cccctgctct ttccgccagg acgcggacgg
    10861 cgggcccgcg cggagcgccc ccgccgccag gcgtgtcccg cccccctttt gccgccctgc
    10921 cggggcgcac ccccggcggc gcaacagcgg cttcacggcc tcccgcagcc cgcccgcagc
    10981 accccgcttc ccg
//
