LOCUS       NZ_LMEQ01000004        18837 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root1310 contig_12, whole genome shotgun sequence.
ACCESSION   NZ_LMEQ01000004
VERSION     NZ_LMEQ01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root1310
  ORGANISM  Streptomyces sp. Root1310
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 241947)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 241947)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140604
            Assembly Name                     :: Root1310
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426425.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:14:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,470
            CDSs (total)                      :: 8,385
            Genes (coding)                    :: 8,123
            CDSs (with protein)               :: 8,123
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 98 of 262
            Pseudo Genes (incomplete)         :: 219 of 262
            Pseudo Genes (internal stop)      :: 20 of 262
            Pseudo Genes (multiple problems)  :: 64 of 262
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:32:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 223110
            Orig. end                         :: 241947
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEQ01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..18837
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..18837
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..18837
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:16698](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..16698
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(157..750)
                     /gene="idi"
                     /locus_tag="ASD48_RS13530"
                     /old_locus_tag="ASD48_35490"
     CDS             complement(157..750)
                     /EC_number="5.3.3.2"
                     /GO_function="GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="idi"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129457.1"
                     /locus_tag="ASD48_RS13530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35490"
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="WP_057601811.1"
                     /transl_table=11
                     /translation="MPITPATATQTSPNGTADAILLELVDENGVTIGTAEKLAAHQPPG
                     QLHRAFSVFLFDERGRLLLQQRALGKYHSPGVWSNTCCGHPYPGEAPFAAAARRTFEEL
                     GVSPSLLAEAGTVRYNHPDPESGLVEQEYNHLFVGLVQSPLRPDAEEVGDTAFVTPAEL
                     AERHAADVFSSWFMTVLDAARPAVRELTGPSAGW"
     misc_feature    complement(895..908)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.43"
                     /tool="antismash"
     gene            complement(938..1879)
                     /locus_tag="ASD48_RS13535"
                     /old_locus_tag="ASD48_35495"
     CDS             complement(938..1879)
                     /GO_function="GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339288.1"
                     /locus_tag="ASD48_RS13535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35495"
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_057601812.1"
                     /transl_table=11
                     /translation="MGAGHDHGHTHTHVPATATAAAAYRGRLRVALSITLAVMVVEIVG
                     GVLADSLALIADAAHMATDALGLGMALLAIQFANRPPSANRTFGLARAEILAALANCLL
                     LLGVGGYVLFEAVQRFFTPATTEGGLMIWFGAFGLVANVISMSLLMRGQAESLNVRGAF
                     LEVAADALGSVAVLISAVVILTTGWQTADPIASLVIGLMIVPRTVKLLRETLDVLLESA
                     PKDVDMAEVRSHILALDGVEDVHDLHAWTITSGMPVLSAHVVVRSDALNAIGHEKMLHE
                     LQGCLGHHFDVEHCTFQLEPSGHAEHEARLCH"
     misc_feature    1973..1987
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.72"
                     /tool="antismash"
     misc_feature    complement(2002..2016)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.35"
                     /tool="antismash"
     misc_feature    2178..2192
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.89"
                     /tool="antismash"
     misc_feature    complement(2312..2326)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.81"
                     /tool="antismash"
     gene            2547..4394
                     /locus_tag="ASD48_RS13540"
                     /old_locus_tag="ASD48_35500"
     CDS             2547..4394
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996269.1"
                     /locus_tag="ASD48_RS13540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35500"
                     /product="DUF5941 domain-containing protein"
                     /protein_id="WP_057601813.1"
                     /transl_table=11
                     /translation="MSTAILTGSPVPGSSIEGDLRSLGFDVRTASDAGDAEALLAAVPG
                     DQRVAVVDARFVGHEHALRLGLTDPRFPLGAIPGAVTAQPAGRQALTRAMARENSANAG
                     TAPGGAPALPKSSVGHVDSVDSLADRVVAALDADGVDVHRPELGSLVAEVPADPQARNE
                     ARQAVAAVDDEAIRLKSAVKARDGFFTTYCISPYSRYVARWCARRGLTPNQVTTASLIT
                     ALIAAACAATGTRGGFVAAGVLLIASFVLDCTDGQLARYSLQYSTLGAWLDATFDRAKE
                     YAYYAGLALGAARGGDDVWALALGAMVLQTCRHVVDFSFNEANHDATANTSPTAALSDK
                     LDSVGWTVWVRRMIVLPIGERWAMIAVLTAATTPRITFYALLVGCAFAATYTTAGRVLR
                     SLTRKARRTDRAAQALADLADSGPLTGLLTRTLPVGIRIPAPLSAAAGVLLLVLAASLD
                     GPGFSVLLAAVLYVLLSADALVRPLKGALDWLVPPLFRAGEYCTVLVLAAKADVDGALP
                     AAFGLVAAVAYHHYDTVYRIRGNAGAPPAWLVRAIGGQDGRTLLVVVLAVLLTASQFTV
                     ALTALAVVVALVVLLESIRFWVAAHRAGAPSVHDEGEPA"
     gene            4391..5143
                     /locus_tag="ASD48_RS13545"
                     /old_locus_tag="ASD48_35505"
     CDS             4391..5143
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064: glucose-1-phosphate
                     adenylyl/thymidylyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983099.1"
                     /locus_tag="ASD48_RS13545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35505"
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="WP_054241363.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.1e-11, bitscore:
                     39.0, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGLVLAAGAGRRLRPYTDSLPKALVPVGPAGIEGEPTVLDLTLG
                     NFAEIGLTEVAVIVGYRKEAVYERKAALEAKYGLKLTLIDNDKAEEWNNAYSLWCGRDA
                     LKDGVILANGDTVHPVSVEHTLLAARGEGRQIILALDTVKSLADEEMKVVVDPDLGVQR
                     ITKLMDPADATGEYIGVTLIEGDAAPALAEALKTVWESDPQQFYEHGYQELVDGGFRID
                     VAPIGDVAWVEIDNHDDLARGREIACQY"
     gene            5131..6192
                     /locus_tag="ASD48_RS13550"
                     /old_locus_tag="ASD48_35510"
     CDS             5131..6192
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DHQ_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1181: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494151.1"
                     /locus_tag="ASD48_RS13550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35510"
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="WP_057601814.1"
                     /sec_met_domain="Fe-ADH (E-value: 5.6e-23, bitscore: 75.0,
                     seeds: 218, tool: rule-based-clusters)"
                     /sec_met_domain="DHQ_synthase (E-value: 6.3e-10, bitscore:
                     32.3, seeds: 76, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVLTRLIPSPVVVDIRPGALDDLVGVLADERISHSGKLAIAVSG
                     GSGARLRERLTPALPGATWYEVGGGTLDDAVQLATDIKAGHFDAVVGLGGGKIIDCAKF
                     AAARVGLPLVAVPTNLAHDGLCSPVATLDNDAGRGSYGVPNPIAVVIDLDVIREAPVRY
                     VRAGIGDAVSNISAIADWELANRIKGEKIDGLAAAIARQAGEAVLRHPGGIGDTGFLQV
                     LAEALVLSGVAMSVSGDSRPSSGACHEINHAFDLLFPKRAAAHGEQCGLGATFAMFLRG
                     AHEESAYMAQVLRRHGLPVLPQEIGFTVDEFVRAVEFAPETRPGRYTILEHLDLKTEQI
                     KDIYADYVKAIGS"
     gene            6170..6949
                     /locus_tag="ASD48_RS13555"
                     /old_locus_tag="ASD48_35515"
     CDS             6170..6949
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049651.1"
                     /locus_tag="ASD48_RS13555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35515"
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="WP_079073746.1"
                     /transl_table=11
                     /translation="MSRPSVAELRPVVHPPGVKDRRSGEHWMGRLYMREVSLRVDRYLV
                     NTRVTPNQLTYLMTVFGVLAAPALLVPGITGAVLGVVCVQMYLLLDCVDGEIARWKKQY
                     SLGGVYLDRVGAYLTDAAVLVGFGLRAADPWGTGRIDWLWAFLGTLAALGAILIKAETD
                     LVGVARHQGGLPPVKESASEPRSSGMALARRAASALQFHRLILGIEASLLILVLAVVDQ
                     LRGDLFFTRLGVAVLAGIALLQTLLHLVSILASSRLK"
     gene            6946..7830
                     /locus_tag="ASD48_RS13560"
                     /old_locus_tag="ASD48_35520"
     CDS             6946..7830
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983096.1"
                     /locus_tag="ASD48_RS13560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35520"
                     /product="glycosyltransferase"
                     /protein_id="WP_057601815.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 8.2e-15,
                     bitscore: 48.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPGKVGAVVITMGNRPDELRALLDSIAKQDGDRVEVVVVGNGS
                     PVPDVPEGVRTIELPENLGIPGGRNVGIEAFGPSGRDVDVLLFLDDDGLLAHHDTAELC
                     RRAFADDPRLGIVSFRIADPDTGVTQRRHVPRLRAADPMRSSRVTTFLGGANAVRTKVF
                     AEVGGLPDEFFYAHEETDLAWRALDAGWMIDYRSDMVLYHPTTAPSRHAVYHRMVARNR
                     VWLARRNLPAPLVPVYLGVWLLLTLVRRPSGSALKAWFGGFREGWSTPCGPRRPMKWRT
                     VWRLTRLGRPPVI"
     gene            7931..8860
                     /locus_tag="ASD48_RS13565"
                     /old_locus_tag="ASD48_35525"
     CDS             7931..8860
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994380.1"
                     /locus_tag="ASD48_RS13565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35525"
                     /product="ABC transporter permease"
                     /protein_id="WP_057601816.1"
                     /transl_table=11
                     /translation="MSETTHDVGVVVTAPPSPDEGLTAAQLAAKYGLAVSGARPPLREY
                     VRQLWGRRHFIMAFSRAKLASQYSQAKLGQLWQVATPLLNAAVYFMIFGLLLNAGRGMT
                     KEVYIPFLVTGVFVFMFTQSSVMTGVRAISGNLGLVRALHFPRASLPISFALQQLQQLL
                     YSMLVLFIVVIGFGSYPDASWALIVPILALQFCFNSGLALIFARAGAKTPDLAQLMPFV
                     MRTWMYASGVMFSIPVFLADKPQWVANILQWNPAAIYMDLMRFALIEEYSAENLPAHVW
                     AVAGGWAVLVALGGFVYFWQAEERYGRG"
     gene            8853..9641
                     /locus_tag="ASD48_RS13570"
                     /old_locus_tag="ASD48_35530"
     CDS             8853..9641
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129465.1"
                     /locus_tag="ASD48_RS13570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35530"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_057601817.1"
                     /transl_table=11
                     /translation="MVEPLAGQRIPTVIADDVHIVYRVNGAKSGRGSATAALSRIVKRG
                     SDESARGVRKVHAVRGVSFVAHRGEAIGLIGSNGSGKSTLLRAIAGLLPPESGKVYTDG
                     QPSLLGVNAALMNDLTGERNVTLGGLAMGMSREQIKERYQEIVDFSGINEKGDFITLPM
                     RTYSSGMQARLRFSIAAAKDHDVLMIDEALATGDAKFRRRSEARVRELREHAGTVFLVS
                     HSNQSIRDTCDRVLWLERGELRLDGPTEEVLKEYEKFSGK"
     gene            10001..10903
                     /gene="hpnC"
                     /locus_tag="ASD48_RS13575"
                     /old_locus_tag="ASD48_35535"
     CDS             10001..10903
                     /EC_number="2.5.1.21"
                     /GO_function="GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019985319.1"
                     /locus_tag="ASD48_RS13575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35535"
                     /product="squalene synthase HpnC"
                     /protein_id="WP_057601818.1"
                     /sec_met_domain="phytoene_synt (E-value: 2.7e-40, bitscore:
                     132.0, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 3.3e-42,
                     bitscore: 138.4, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPAAPRIGDPERGTLDKAADENFPVAPFFLPRAWRDDLMAVYG
                     FARLVDDIGDGDLAPGGADARLLGVSATAAEDRLALLDAFEADLRRVFDGTPRHPLLRR
                     LQPTVRRRSLGPEPFLGLIAANRQDQLVTRYETYDDLLAYCELSANPVGRLVLAVTGAA
                     TPERIRRSDAICTALQIVEHLQDVSEDLSRDRVYLPAADMKRFHVKESDLAMNTAGASV
                     RALVAFEAQRARDLLNEGAPLVGSVHGRLRLLLAGFVAGGSAAIKAIAAAGYDVLPGPP
                     KPGRLRLLREVGVTLRGEG"
     gene            10900..11850
                     /gene="hpnD"
                     /locus_tag="ASD48_RS13580"
                     /old_locus_tag="ASD48_35540"
     CDS             10900..11850
                     /EC_number="2.5.1.103"
                     /GO_function="GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129467.1"
                     /locus_tag="ASD48_RS13580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35540"
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="WP_057601819.1"
                     /sec_met_domain="phytoene_synt (E-value: 6.6e-58, bitscore:
                     189.7, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 1.9e-69,
                     bitscore: 227.9, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRAVEPSPHVSAPVLAAYGYCETVTGQQARNFAYGIRLLPTPKR
                     RAMSALYAFSRRVDDIGDGALDGEVKTARLEETRALLTRVRAGEVEEDDTDPVAVALAH
                     TARVFPIPLRGLDELIDGVLMDVRGETYETWDDLKVYCRCVAGAIGRLSLGVFGTERGA
                     RGAERAPEYADTLGLALQLTNILRDVREDAEGGRTYLPADDLAKFGCSTGFDGPKPPEG
                     SDFVGLVHFEVRRARALFAEGYRLLPLLDRRSGACVAAMAGIYRRLLDRIERDPEAVLR
                     GRVSLPGREKAFVAVRGLSGLDARHVSRRTVRRHP"
     gene            11999..13420
                     /gene="hpnE"
                     /locus_tag="ASD48_RS13585"
                     /old_locus_tag="ASD48_35545"
     CDS             11999..13420
                     /EC_number="1.17.8.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129468.1"
                     /locus_tag="ASD48_RS13585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35545"
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="WP_057601820.1"
                     /sec_met_domain="DAO (E-value: 2.1e-10, bitscore: 34.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDEQLADAAEASGPSGRTAVVVGGGLAGVTAALALADAGVRVTL
                     LEGRPRLGGLAFSFTRGELTVDNGQHVYLRCCTAYRWFLDRIEGSALAPLQDRLDVPVV
                     DVAAPEGRRLGRLRRDALPVPLHLGRSLATYPHLSLAERARVGRAALALKGLDLADPRL
                     DAQDFGSWLTAHGQSARAVEALWDLVGVATLNAVAGDSSLGLAAMVFKTGLLSDPGAAD
                     IGWAHVPLGELHDRLARKALDSAGVRTEVRTRVTSLSSDDNGTWSVQVPGETLRADTVV
                     LAVPQREAYDLLPAGALDHAERLLEIGTAPILNVHVVYDRKVLDKPFFAALGTPVQWVF
                     DRTAASGLREGQYLALSQSAAHDEIDEPVAALRERYLPELERLLPRTRGAGVRDFFVTR
                     ERTATFAPTPGVGRLRPGARTRTPGLYLAGAWTATGWPATMESAVRSGVSAADAALSAL
                     GRPRPRRLFEFEEAA"
     gene            13531..14559
                     /locus_tag="ASD48_RS13590"
                     /old_locus_tag="ASD48_35550"
     CDS             13531..14559
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129469.1"
                     /locus_tag="ASD48_RS13590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35550"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_057601821.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.8e-57, bitscore:
                     188.3, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVTALLERGRTLATPVLRAAVDRLAPPMDTVAAYHFGWIDAHGN
                     PTDGDGGKAVRPALAVLSAEVAGAAPEVGVPGAVAVELVHNFSLLHDDLMDGDEQRRHR
                     DTVWKVHGPAQAILVGDALFALAGEILLELGTAEAARATHRLTVATRALIDGQAQDISY
                     EHRDRVSVEECLEMEGNKTGALLACASSIGAVLGGADRHTADALEKYGYHLGLAFQAVD
                     DLLGIWGDPESTGKQTWSDLRQRKKSLPVVAALAAGGSASERLGEILAADAKSSDFETF
                     SEGEFAARAALIEEAGGRDWTAGEARRQHAVALDALNAVDMSEQVRDRFTALADFVVVR
                     KR"
     misc_feature    14643..14656
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.64"
                     /tool="antismash"
     gene            14677..16698
                     /gene="shc"
                     /locus_tag="ASD48_RS13595"
                     /old_locus_tag="ASD48_35555"
     CDS             14677..16698
                     /EC_number="5.4.99.17"
                     /GO_function="GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="shc"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327863.1"
                     /locus_tag="ASD48_RS13595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35555"
                     /product="squalene--hopene cyclase"
                     /protein_id="WP_057601822.1"
                     /sec_met_domain="T2TS (E-value: 1.6e-152, bitscore: 502.5,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATTDGSTGALPPRAAAASETDIIIPVAAGVQEAATRAMQRATD
                     FLLAGQDAEGWWKGDLETNVTMDAEDLLLRQFLGIRDEATTRAAALFVRGEQREDGTWA
                     TFYGGPPDLSATVEAYVALRLAGDAPEAPHMRRASGWIREQGGIASARVFTRIWLALFG
                     WWKWEDLPELPPELLFFPKWFPLSIYNFGCWARQTIVPLTVVSAKRPVRPAPFPLDELH
                     TDPARPNPARPLAPVNSWDGLFQRLDKVVRGYRSVAVRRLRGAALNAAARWIIERQEND
                     GAWGGIQPPAVYSVIALHLLGYDLRHPVMRAGLESLDRYAVWREDGARMIEACQSPVWD
                     TCLATIALADAGLPADHPQLVKAADWMLGEQVVRPGDWSVSRPQLPPGGWAFEFHNDNY
                     PDIDDTAEVVLALRRVKHHDPERMDRAIARGVRWNLGMQSKNGGWGAFDVDNTSPFPNR
                     MPFCDFGEVIDPPSADVTAHVVEMLAVEGLSHDPRTRRGIEWLLAEQEPDGSWFGRWGV
                     NYVYGTGSVVPALTAAGLPAQHPAIRRAVSWLERVQNDDGGWGEDLRSYQDATRWSGRG
                     ASTASQTAWALMALLAAGERDSKAVERGVAWLAQTQREDGSWDEPYFTGTGFPWDFSIN
                     YHLYRQVFPLTALGRYVHGEPFSKQPATHAASAEVKGS"
     gene            16698..17339
                     /locus_tag="ASD48_RS13600"
                     /old_locus_tag="ASD48_35560"
     CDS             16698..17339
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431357.1"
                     /locus_tag="ASD48_RS13600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35560"
                     /product="1-hydroxy-2-methyl-2-butenyl 4-diphosphate
                     reductase"
                     /protein_id="WP_057601823.1"
                     /transl_table=11
                     /translation="MSTQSVPSPLLIACALGIEHLALRMGERGGADGPVTVLRTGMGPK
                     AAERSVTRLLADPLFAGAAVLATGFCAGLAPGMHPGDLVVAEETRDPRGSVPCVGTELL
                     VKELARTLPGRTVHTGPLTGSDHVVRGHERADLLATGAIALDMESAATLLSAVRAEPRP
                     VAAVRVVVDAPEHELVRIGTVRGGISAFRVLRSVLPAFFEWHRNVLLPRR"
     gene            17345..18367
                     /gene="hpnH"
                     /locus_tag="ASD48_RS13605"
                     /old_locus_tag="ASD48_35565"
     CDS             17345..18367
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386169.1"
                     /locus_tag="ASD48_RS13605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35565"
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="WP_057601824.1"
                     /sec_met_domain="PF04055 (E-value: 2.4e-18, bitscore: 60.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 5.7e-10, bitscore: 32.7,
                     seeds: 32, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAMPLRQSIKVATYLAEQKLRKREKFPLIVELEPLFACNLKCEGC
                     GKIQHPAGVLKQRMPVAQAVGAVLESGAPMVSIAGGEPLMHPQIDEIVRQLVAKRKYVF
                     LCTNAMLLRKKLDKFTPSPYFAFAVHIDGLRERHDESVAKEGVFDEAVAAIKEAKQRGF
                     RVTTNSTFFNTDTPQTIIEVLNYLNDDLKVDEMMISPAYAYEKAPDQEHFLGVEQTREL
                     FKKAFSGGNRRRWRLNHSPLFLDFLEGKADFPCTAWAIPNYSLFGWQRPCYLMSDGYVP
                     TYRELIEETDWDKYGRGKDPRCANCMAHCGYEPTAVLATMGSLKESLRALRETVNGNRE
                     "
     gene            18372..>18837
                     /locus_tag="ASD48_RS13610"
                     /old_locus_tag="ASD48_35570"
     CDS             18372..>18837
                     /EC_number="1.17.7.3"
                     /GO_function="GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA]"
                     /GO_process="GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011031167.1"
                     /locus_tag="ASD48_RS13610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_35570"
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="WP_199875782.1"
                     /transl_table=11
                     /translation="MTAVSLGVPGMPVRPVASRRESRRIQVGPVAVGGGAPVSVQSMTT
                     TRTSDIGATLQQIAELTASGCQIVRVACPTQDDADALATIARKSQIPVIADIHFQPKYV
                     FAAIEAGCAAVRVNPGNIKQFDDKVREIAKAAKEHGTPIRIGVNAGSLDRR"
ORIGIN
        1 gaggccgacc acggtcgcac gggcggccgg ggactgctgc tggtgcggga gatcaccctg
       61 gaggcgggcg gggtctgcga cgtcgagcac acgtcgggcg gcggcaaggt gatctgggcc
      121 gccctgccgc tgaagcccgt gcgggtgccc tgagggtcac cagccggccg acggccccgt
      181 cagctctctg accgccgggc gggcggcgtc caggacggtc atgaaccagg acgagaacac
      241 gtccgcggca tgccgctccg ccagctcggc gggcgtcacg aaggccgtgt cgccgacctc
      301 ctccgcgtcc ggccgcagcg gggactgcac cagccccacg aacaggtggt tgtactcctg
      361 ctcgaccaga cccgactccg ggtccgggtg gttgtagcgg accgtgcccg cctcggcgag
      421 cagcgacggg gagaccccga gctcctcgaa ggtgcgccgc gcggccgccg cgaacggtgc
      481 ctcaccgggg taggggtgac cgcagcaggt gttggaccac accccggggg agtggtactt
      541 gccgagcgcc cgctgctgga gcagcagccg gccccgctcg tcgaagagga acacggagaa
      601 ggcgcggtgc agctgccccg gcggctgatg ggcggccagc ttctccgcgg tgccgatcgt
      661 gacgccgttc tcgtcgacca gctccagcaa gatcgcgtcc gcggtgccgt tcggcgacgt
      721 ctgtgtcgcg gtggcaggtg tgatcggcat acccatcctt agcatcggtc ctcgcgtccc
      781 cagtctgccg tacgcgtccg gcactcccgg cacttcgcgc gaaagcgcgc atgtcccgca
      841 cggcaagggg cccggtaggt gatcttgccc ggcgccggga tcggagaacc gtccggaacg
      901 tgtgtgcgga tcatgcggcg cgggcgtcgg cgcgcgctca gtggcagagc cgggcctcgt
      961 gttccgcgtg cccgctcggc tccagctgga aggtgcagtg ctccacgtcg aagtggtggc
     1021 ccaggcagcc ctggagctcg tgcagcatct tctcgtggcc gatcgcgttc agcgcgtccg
     1081 agcgcaccac cacgtgcgcc gacagcaccg gcatcccgga ggtgatggtc cacgcgtgca
     1141 gatcgtggac gtcctccacc ccgtccaggg cgagtatgtg ggagcgcacc tccgccatgt
     1201 cgacgtcctt gggggccgac tccagcagca cgtcgagcgt ctcgcgcagc agcttcacgg
     1261 tgcgcggcac gatcatcagg ccgatgacga gcgaggcgat cgggtcggcg gtctgccagc
     1321 cggtggtcag gatcaccacc gccgagatca gcaccgccac cgaccccagg gcgtccgccg
     1381 ccacctccag gaacgccccg cgcacgttca ggctctcggc ctggccgcgc atcagcagcg
     1441 acatcgagat cacgttcgcg accagaccga acgcgccgaa ccagatcatc agcccgccct
     1501 cggtggtggc gggggtgaag aaccgctgga cggcctcgaa caggacgtag ccgcccaccc
     1561 cgagcagcag cagacagttg gcgagggcgg ccaggatctc ggcgcgggcc agtccgaagg
     1621 tgcggttggc gctcggcggc cggttggcga actggatggc cagcagcgcc atgcccagcc
     1681 ccagcgcgtc cgtcgccatg tgcgcggcgt ccgcgatcag cgcgagggag tccgcgagca
     1741 ccccgccgac gatctcgacg accatgacgg cgagcgtgat cgacagggcg acgcgcagtc
     1801 tgccccggta cgccgccgcg gccgtcgcgg tggccggcac gtgggtgtgc gtatggccgt
     1861 ggtcgtgccc agcccccatg gaagcagccc ttctgtggtc cgtcgtgggc cgtcgcgttc
     1921 tgccccgcga tcacagtgaa ctacgggtgg ggggtatccg gcaacgcggc actgaacacc
     1981 gttgtcatgt gccctgacct gcgaaaacga atcgcaggtc agcgcgtccc cgcctgctgc
     2041 tcgccgtgga tgcggccgtc ggccccggca cggcgggagg ccgcccgggg ccgcgaagcg
     2101 ctccgcggcc ttgtcgcgac cccggtcgcg gcacccgtgg gggacgcgtt ccgtacccct
     2161 ggtgcccgga agtcaccggg atatccgttc cggcggtgat gaggcatgtc atgccggaag
     2221 ttcccgtccc gaccggtacg ggcacaccgg gccggtggac aatgtcctgg atgtcggctc
     2281 tcgggggcac ggtttgtcgc ccgggccgcc gatcaccgat gatgatctcg gcaaaggccg
     2341 gttcaagccg agttcaagcc gtgccgccgg accacggcga tcgtgcctga acgggcggtg
     2401 aacacggcgt tcccggcatc cgatagcctc tgccgacatg ccgcccgcct ggtgcggcca
     2461 ccggtgaagc cgccggtgcg gcccaggggc gcctccatcg tgtacatgac cggcgtcaac
     2521 gcgccggacc cagggagtga gttcggttgt cgaccgccat cctcaccggc tcgccggtcc
     2581 ccggatcgtc gatcgagggc gatctgcggt ccctcggctt cgacgtccgg accgcctccg
     2641 acgccggtga cgccgaggcc ctcctcgccg ccgtccccgg cgaccagcgc gtcgccgtcg
     2701 tggacgcccg cttcgtgggt cacgagcacg cgctgcgtct cggcctcacc gacccccgtt
     2761 tcccgctcgg cgcgatcccg ggggcggtga ccgcgcagcc ggccggccgg caggccctga
     2821 cccgcgcgat ggccagggag aactcggcga acgccggcac cgcccccggg ggtgctcccg
     2881 cgctgccgaa gtcgagcgtg ggacacgtcg acagcgtcga cagcctcgcc gaccgggtcg
     2941 tcgccgccct cgacgccgac ggcgtcgacg tgcaccgccc cgaactgggc agcctggtcg
     3001 cggaggtccc ggccgacccg caggcccgca acgaggcccg gcaggccgtc gccgccgtcg
     3061 acgacgaggc gatccgcctg aagtcggccg tcaaggcgcg cgacggcttc ttcaccacct
     3121 actgcatcag cccgtactcg cgctatgtcg cccgctggtg cgcccgccgc ggcctcaccc
     3181 ccaaccaggt caccaccgcc tcgctgatca ccgcgctgat cgcggcggcc tgcgcggcca
     3241 ccggcacccg gggcgggttc gtcgcggccg gcgtgctgct gatcgcgtcc ttcgtgctgg
     3301 actgcaccga cggccagctc gcccgctact ccctgcagta ctccacgctc ggcgcctggc
     3361 tcgacgccac cttcgaccgc gccaaggagt acgcctacta cgcgggtctc gcgctcggcg
     3421 cggcccgcgg cggcgacgac gtgtgggccc tggcgctggg cgcgatggtc ctgcagacct
     3481 gccggcacgt cgtggacttc tccttcaacg aggccaacca cgacgccacc gcgaacacca
     3541 gccccaccgc cgccctctcc gacaagctcg acagcgtcgg ctggacggtc tgggtgcggc
     3601 ggatgatagt cctgcccatc ggtgaacgat gggccatgat cgccgttctc accgctgcca
     3661 ccaccccccg catcaccttc tacgcgctgc tcgtcggctg cgcgttcgcg gccacctaca
     3721 ccacggcggg ccgggtcctg cgctcgctga cccgcaaggc ccggcggacc gaccgggcgg
     3781 cgcaggcact ggcggacctc gccgacagcg gcccgctcac cggactgctg acccgcacgc
     3841 tccccgtggg catccgcatc ccggccccgc tcagcgcggc cgccggcgtc ctcctgctgg
     3901 tcctcgccgc ctcgctcgac gggccgggct tctcggtcct cctggccgcc gtcctgtacg
     3961 tcctgctctc cgccgacgcc ctcgtgcgcc ccctcaaggg cgccctcgac tggctggtcc
     4021 cgccgctctt ccgggccggg gagtactgca ccgtcctcgt cctcgcggcc aaggccgacg
     4081 tggacggagc gcttcccgcg gctttcgggc tggtcgccgc cgtcgcctac catcactacg
     4141 acacggtgta ccgcatccgc ggcaacgccg gagcgccgcc ggcctggctg gtgcgcgcca
     4201 tcggggggca ggacgggcgc acgctgctcg tcgtcgtgct ggccgtgctg ctcacggcct
     4261 cgcagttcac ggtcgcgctc acggccctcg ccgtggtcgt cgccctcgtg gtgctcctcg
     4321 agagcatccg cttctgggtc gccgcccacc gggcaggcgc cccgtccgta cacgatgaag
     4381 gagaacccgc atgatcggcc tcgtgctggc ggccggcgcc ggacggcgtc tgcgccccta
     4441 caccgacagc ctgcccaagg cgctggtgcc ggtggggccc gcgggcatag agggcgagcc
     4501 cacggtcctg gacctgacgc tcggcaactt cgccgagatc ggtctgaccg aggtcgcggt
     4561 catcgtcggc taccgcaagg aggccgtgta cgagcgcaag gcggcgctgg aggcgaagta
     4621 cggcctcaag ctcaccctca tcgacaacga caaggccgag gagtggaaca acgcctactc
     4681 cctgtggtgc ggccgtgacg ccctcaagga cggcgtcatc ctcgccaacg gcgacaccgt
     4741 ccacccggtg tccgtcgagc acacgctgct cgccgcccgc ggcgagggca ggcagatcat
     4801 cctcgcgctc gacacggtga agtccctcgc cgacgaggag atgaaggtcg tcgtcgaccc
     4861 cgacctgggc gttcagcgga tcaccaagct gatggacccg gccgacgcca ccggcgagta
     4921 catcggcgtc accctcatcg agggcgacgc ggcccccgcc ctggccgagg cgctcaagac
     4981 cgtgtgggag agcgacccgc agcagttcta cgagcacggc taccaggagc tcgtcgacgg
     5041 cggcttccgc atcgacgtgg ccccgatcgg cgatgtcgcg tgggtcgaga tcgacaacca
     5101 cgacgatctc gcccgtggac gggagatcgc gtgccagtac tgacccggct gatcccctca
     5161 ccggtcgtcg tggacatccg cccgggtgcc ctcgacgacc tggtcggggt tctcgccgac
     5221 gagcgcatct cgcactcggg caagctcgcg atcgccgtca gcggcggctc gggcgcccgg
     5281 ctgcgcgagc gcctcacccc cgccctgccc ggcgccacct ggtacgaggt cggcggcgga
     5341 accctcgacg acgccgtcca gctggccacc gacataaagg ccggccactt cgacgcggtc
     5401 gtcggcctgg gcggcggcaa gatcatcgac tgtgccaagt tcgccgccgc ccgcgtgggc
     5461 ctcccgctgg tcgccgtgcc gacgaacctc gcgcacgacg gtctgtgctc gccggtcgcc
     5521 accctcgaca acgacgcggg gcgcggctcc tacggtgtgc cgaacccgat cgccgtcgtg
     5581 atcgacctgg acgtcatccg cgaggccccg gtccgctatg tgcgggccgg catcggtgac
     5641 gccgtctcca acatctccgc catcgcggac tgggagctcg ccaaccggat caagggcgag
     5701 aagatcgacg gcctggccgc cgcgatcgcc cgccaggcgg gcgaggcggt gctgcgccac
     5761 ccgggcggca tcggggacac cggcttcctc caggtactgg ccgaggcgct ggtgctcagc
     5821 ggcgtcgcga tgtcggtgtc gggcgactcc cgcccgtcct ccggcgcctg ccacgagatc
     5881 aaccacgcct tcgacctgct cttccccaag cgggcggccg cccacggcga gcagtgcgga
     5941 ctcggcgcga ccttcgcgat gttcctgcgc ggagcgcacg aggagtcggc ctacatggcc
     6001 caggtgctgc gccgccacgg ccttccggtc ctgccgcagg agatcggctt cacggtggac
     6061 gagttcgtcc gtgccgtgga gttcgctccc gagacccggc cgggccgcta cacgatcctc
     6121 gaacacctcg acctcaagac cgaacagatc aaggacatct acgccgacta tgtcaaggcc
     6181 atcggtagct gaactcagac cggtcgtcca ccctccgggg gtgaaggacc ggcgcagcgg
     6241 tgagcactgg atggggcgcc tctacatgcg tgaggtgtcc ctgcgggtcg accgctacct
     6301 ggtcaacacc agggtcacgc ccaaccagct cacgtacctg atgaccgtct tcggggtgct
     6361 cgccgccccg gccctgctgg tgccggggat cacgggcgcc gtgctcggcg tggtgtgcgt
     6421 ccagatgtat ctgctgctgg actgcgtgga cggcgagatc gcccgctgga agaagcagta
     6481 ctcgctgggc ggggtctatc tggaccgggt cggcgcgtat ctcaccgacg ccgccgtcct
     6541 ggtcggcttc ggcctgcgcg ccgccgaccc gtggggcacc ggacgcatcg actggctctg
     6601 ggccttcctc ggcacgctgg ccgcgctcgg cgcgatcctg atcaaggcgg agaccgacct
     6661 cgtcggcgtc gcccggcacc agggcggtct gcctccggtg aaggagtcgg cgtccgaacc
     6721 gcgctcctcc gggatggcgc tggcccgccg ggccgcctcg gcgctccagt tccaccggct
     6781 gatcctcggt atcgaggcgt ccctgctgat cctggtgctg gcggtcgtcg accagctgcg
     6841 cggcgacctg ttcttcaccc ggctcggcgt ggccgtgctg gccggcatcg cgctgctcca
     6901 gaccctgctg cacctggtgt ccatcctcgc gtcgagcagg ctgaagtgag cgcgcccggg
     6961 aaggtcggcg cggtcgtcat caccatgggc aaccgccccg acgagctgag agcgctgctg
     7021 gactcgatcg ccaagcagga cggcgaccgg gtcgaggtgg tcgtggtcgg caacggctcg
     7081 cccgtcccgg acgtccccga gggcgtcagg acgatcgagc tgcccgagaa cctcggtatc
     7141 cccggcggcc gcaacgtcgg catcgaggcc ttcgggccca gcggccggga cgtcgacgtc
     7201 ctgctgttcc tggacgacga cggtctcctg gcccaccacg acacggccga gctgtgccgc
     7261 cgggccttcg ccgacgatcc gaggctgggc atcgtcagct tccgcatcgc cgacccggac
     7321 accggtgtca cccagcgccg ccacgtaccc cggctgcgcg ccgcggaccc gatgcgctcc
     7381 tcccgggtca ccaccttcct cgggggcgcc aacgccgtgc gcacgaaggt cttcgcggag
     7441 gtgggcggac tcccggacga gttcttctac gcccacgagg agaccgacct ggcatggcgg
     7501 gccctcgacg cgggctggat gatcgactac cggtccgaca tggtgctgta ccacccgacg
     7561 accgcgccct cgcggcacgc ggtgtaccac cgcatggtcg cccgcaaccg ggtctggctg
     7621 gcccggcgca accttcccgc cccgctggtc cccgtctatc tcggggtgtg gctgctgctc
     7681 accctggtgc gccgtccctc ggggtccgcg ctgaaggcct ggttcggcgg tttccgggaa
     7741 ggctggagca ctccgtgcgg acctcgtaga cccatgaagt ggcgtacggt gtggcggctg
     7801 acccgactgg gccggccccc ggtgatctga caagctcgtc cctgagggca ccccgggccg
     7861 taccgcgggc cccccggctc atgccggcct cgtcaaggct gcgcatcccg aagacgaaag
     7921 tttcctacct gtgagtgaga caacgcatga cgtcggagtc gtggtgactg cgcctccgtc
     7981 gcccgacgag ggcctcacgg cggcacagct ggccgccaag tacgggctgg ccgtgagcgg
     8041 cgcccggcct ccgctgcggg agtacgtccg gcagctctgg gggcgccggc acttcatcat
     8101 ggcgttctcc cgggcgaaac tggcctccca gtacagccag gccaagctcg gccagctgtg
     8161 gcaggtggcc acaccgctgc tgaacgcggc cgtgtacttc atgatcttcg gcctgctcct
     8221 caacgccggc cgcggcatga cgaaggaggt gtacatcccc ttcctcgtca cgggcgtctt
     8281 cgtgttcatg ttcacgcaga gctcggtgat gaccggcgtc cgggcgatct cgggcaatct
     8341 cggcctggtc cgcgccctgc atttcccgcg tgcctcgctg cccatctcgt tcgccctcca
     8401 gcaactccag cagctgctgt actcgatgct cgtgctgttc atcgtggtga tcggcttcgg
     8461 cagctacccg gacgcctcct gggcgctgat cgtcccgatc ctcgccctcc agttctgctt
     8521 caactccggt ctggcgctga tcttcgcccg ggcgggcgcg aagaccccgg acctggcgca
     8581 gctgatgccg ttcgtgatgc ggacgtggat gtacgcgtcc ggcgtgatgt tctccatccc
     8641 cgtcttcctc gcggacaagc cgcagtgggt ggccaacatc ctgcagtgga acccggccgc
     8701 catctacatg gacctgatgc gcttcgcgct catcgaggag tacagcgccg agaacctccc
     8761 cgcgcacgtc tgggcggtcg cgggcggctg ggccgtcctc gtcgccctcg gcggcttcgt
     8821 gtacttctgg caggcggagg agaggtacgg ccgtggttga gccgctcgcg ggacagcgga
     8881 tcccgaccgt catcgccgac gacgtccaca tcgtctaccg cgtcaacggc gccaagtccg
     8941 gccggggcag cgccaccgcg gcgctcagcc gcatcgtcaa gcgcggttcc gacgagtcgg
     9001 cacgcggggt gcgcaaggtg cacgccgtgc gcggcgtctc cttcgtcgcc caccgcggcg
     9061 aggccatcgg cctgatcggc tccaacggct ccggcaagtc cacgctgctg cgcgccatcg
     9121 ccggtctgct gccgccggag agcggcaagg tctacaccga cggccagccc tcgctgctcg
     9181 gcgtcaacgc ggccctgatg aacgacctca ccggcgagcg caacgtcacc ctcggcgggc
     9241 tcgccatggg catgtcccgc gagcagatca aggagcgcta ccaggagatc gtcgacttct
     9301 ccggcatcaa cgagaagggc gacttcatca ccctgccgat gcgcacctac tcctccggca
     9361 tgcaggcccg actgcgcttc tccatcgcgg ccgccaagga ccacgacgtc ctgatgatcg
     9421 acgaggccct ggccaccggc gacgccaagt tccgcaggcg ctccgaggcc cgggtgcggg
     9481 agctgcgcga gcacgccggc acggtgttcc tggtcagcca cagcaaccag tccatccgcg
     9541 acacctgtga ccgggtcctg tggctggaac gcggcgaact gcggctggac ggcccgaccg
     9601 aagaggttct caaggagtac gagaagttct cgggcaagta gggcgattcg gccagggccc
     9661 cgccggaact gtccggcggg gccctgcgtc tgcaaaggaa acgtcaactc cgcgtcagcc
     9721 aaggaatctt gacgccaatc ggtgtgttgt tgtgatgtcc cggacacccc cgcgaaccgt
     9781 gtcgcgttgt acaacgtaag ctggaccggc ccccgaatcg cgccaagtgg ggcgaaaacg
     9841 tgcagtgcca ccgtcgggga cgctgcgcgg gcggctcggc ggcgtgtccg aaatagtgtg
     9901 tattagatcg gcagtgtaga acgggagatg tgacggcgat ggctacggaa actccccagg
     9961 tcgacgcgag ccgtgccgtc cccgctccgg acggccgacg atgacggcgc ccgccgcgcc
    10021 gcgcatcggc gacccggagc gcggcacgct cgacaaggcc gcggacgaga acttcccggt
    10081 ggcccccttc ttcctgccca gggcctggcg cgacgacctg atggccgtgt acggcttcgc
    10141 ccggctcgtc gacgacatcg gcgacggcga cctcgccccc ggcggcgccg acgcccggct
    10201 gctcggcgtg tcggccaccg ccgccgagga ccgcctcgcc ctgctggacg ccttcgaggc
    10261 cgatctgcgc cgggtcttcg acggcacccc gcggcacccc ctgctgcgtc gcctccagcc
    10321 cactgtccgc cgtcgctcgc tcggccccga gccctttctc ggcctgatcg ccgccaaccg
    10381 gcaggaccag ctcgtcacgc gctacgagac ctacgacgac ctcctcgcgt actgcgagct
    10441 gtccgccaat cccgtgggcc ggctggtcct cgccgtcacc ggcgccgcca cccccgagcg
    10501 gatccgccgc tccgacgcga tctgcaccgc gctgcagatc gtcgagcatc tccaggacgt
    10561 gtccgaggac ctgagccgcg accgtgtcta tctgcccgcc gcggacatga agcgctttca
    10621 cgtcaaggaa tcggatctgg ccatgaacac cgcgggcgcg tcggtgcgcg cgctggtcgc
    10681 gttcgaagcg caacgcgctc gcgatctgct gaatgaaggc gcccccctcg tgggtagcgt
    10741 tcacggcagg ctgaggctgt tgctcgcggg tttcgtggcg gggggaagcg cggcgatcaa
    10801 ggcgatcgcc gccgccggat acgacgtgct tcccggcccg cccaagcccg gcaggctccg
    10861 gctgctgcgc gaggtgggcg tgaccctgcg aggagagggg tgatccgggc cgtggagccg
    10921 tcaccacacg tgtccgcgcc ggtactcgcc gcctacggct actgcgagac cgtcaccgga
    10981 cagcaggccc gtaacttcgc gtacggcatc cggctgctgc ccacgcccaa gcgccgcgcg
    11041 atgtcggcgc tgtacgcctt ctcgcgccgc gtcgacgaca tcggcgacgg cgcactggac
    11101 ggggaggtca agaccgccag gctggaggag acccgggcgc tgctcacccg ggtccgcgcg
    11161 ggcgaggtcg aggaggacga caccgacccc gtcgcggtcg ccctcgcgca cacggcccgc
    11221 gtcttcccca tcccgctgcg cggcctggac gagctgatcg acggcgtcct gatggacgtg
    11281 cgcggcgaga cctatgagac ctgggacgac ctgaaggtgt actgccgctg cgtggcgggc
    11341 gccatcgggc ggctctcgct cggggtgttc ggcaccgaac ggggggcgcg cggcgccgag
    11401 cgcgcgccgg agtacgccga cacgctcggg ctcgcgctcc agctcacgaa catcctcaga
    11461 gacgtccgcg aggacgccga gggcggccgc acctatctgc ccgccgacga cctcgcgaag
    11521 ttcggctgtt cgaccgggtt cgacgggccg aagccgccgg agggatccga cttcgtgggg
    11581 ctcgtgcact tcgaggtgcg gcgggcccgc gccctcttcg ccgaggggta ccggctgctt
    11641 cccctgctcg accggcgcag cggcgcctgt gtcgccgcca tggcgggcat ctaccgccgt
    11701 ctcctcgacc gcatcgagcg cgaccccgag gccgtgctgc gcggcagggt ctcgctgccc
    11761 ggacgggaga aggccttcgt ggccgtacgc gggctgtcgg gcctggacgc ccggcacgtg
    11821 tcgcggcgga ccgtcaggag gcacccgtga cggacgagac cggtgacaag gtgggccagg
    11881 gtgattccac cggcgaaaag cggcacgcaa ccctccggtg cggcgcggcg tccctgactg
    11941 cgacggccag cggtgcagag ttcaaacacc atggccggtc cgcacgggag ggtgcaggat
    12001 gaacgacgag caactcgcgg acgccgcgga agcgtccggc ccttcggggc gtaccgccgt
    12061 cgtcgtcggg ggagggctcg ccggggtgac cgccgcgctc gcgctcgccg acgcgggcgt
    12121 acgcgtcacg ctgctcgaag gccggccccg gctcggcggc ctggccttct ccttcacgcg
    12181 cggcgaactg accgtggaca acggacagca cgtgtacctg cgctgctgca ccgcctaccg
    12241 ctggttcctc gaccgcatcg agggcagcgc gctggcgccc ttgcaggatc gtctcgacgt
    12301 gcccgtcgtc gacgtggccg cgcccgaggg acgacggctc ggcaggctgc ggcgcgacgc
    12361 gctgcccgtg cccctgcacc tggggcgcag cctcgccacg tatccgcatc tctcgctcgc
    12421 cgaacgggcc agggtgggcc gtgccgcgct cgcgctcaag gggctcgacc tcgccgatcc
    12481 gcgactggac gcacaggact tcggcagctg gctgaccgcg cacggtcagt cggcgcgcgc
    12541 cgtcgaggca ctctgggacc tggtcggggt cgccacgctc aacgcggtcg ccggagactc
    12601 ctcgctgggg ctcgccgcga tggtgttcaa gaccggtctg ctgtccgacc cgggcgccgc
    12661 cgacatcggc tgggcgcatg tcccgctggg cgaactgcac gaccggctcg cccgcaaggc
    12721 gctcgactcc gccggcgtgc gtaccgaggt ccgtacacgc gtcacctccc tctcttccga
    12781 cgacaacggg acgtggagcg ttcaggttcc cggcgagacg ctccgcgcgg acacggtcgt
    12841 cctcgccgta ccgcagcgcg aggcctacga cctgctcccc gccggggcgc tcgaccacgc
    12901 cgagcggctg ctggagatcg gcaccgcgcc catcctcaac gtgcacgtcg tctacgaccg
    12961 caaggtgctc gacaagccct tcttcgcggc gctcggcacg cccgtgcagt gggtcttcga
    13021 ccgcaccgcc gcctccgggc tgcgcgaggg ccagtacctc gccctgtccc agtccgcggc
    13081 gcacgacgag atcgacgaac ccgtggccgc gctgcgcgag cgctatctgc ccgagctgga
    13141 acggctcctg ccgcgcacgc gcggcgccgg ggtgcgggac ttcttcgtga cccgggaacg
    13201 cacggcgacg ttcgccccca cccccggcgt cgggcgcctt cggcccggcg cccgcaccag
    13261 aacccccggc ctgtacctgg ccggagcgtg gaccgccaca gggtggcccg cgaccatgga
    13321 gagtgcggtc cgcagcggtg tcagcgcggc ggacgccgcg ctgagcgccc tcggccggcc
    13381 ccgcccccgc cgcctcttcg agttcgagga ggcggcctga tgctcgatca gcacggctcg
    13441 gcacaccccc gcaccctctc cagcaccgca acaagaggag aaaacgtgcc cactgtgccc
    13501 ccggccacgc cggccgctcg gaggaccgcg gtggacgtga ccgcgctcct ggagcgcggc
    13561 cgcaccctgg ccacaccggt gctgcgggcg gccgtcgacc gtctggcacc gcccatggac
    13621 acggtcgccg cctaccactt cggctggatc gacgcccacg gcaacccgac cgacggtgac
    13681 ggcggcaagg ccgtgcgtcc cgccctcgcc gtgctctccg ccgaggtcgc cggggcggcc
    13741 cccgaggtcg gagtccccgg cgcggtcgcc gtcgagctgg tccacaactt ctcgctcctg
    13801 cacgacgacc tgatggacgg cgacgagcag cgcaggcacc gcgacaccgt ctggaaggtg
    13861 cacggccccg cccaggccat cctggtcggc gacgccctgt tcgccctcgc gggcgagatc
    13921 ctcctcgaac tcggcacggc cgaggcggcc cgggcgaccc accgcctgac cgtcgccacc
    13981 cgtgccctca tcgacggcca ggcccaggac atctcctacg agcaccgcga ccgggtcagc
    14041 gtcgaggagt gcctggagat ggagggcaac aagaccggtg ccctgctggc ctgcgccagt
    14101 tccatcggcg ccgtgctcgg cggcgcggac cggcacaccg ccgacgccct ggagaagtac
    14161 ggctaccacc tcggccttgc cttccaggcc gtggacgacc tgctcggcat ctggggcgac
    14221 ccggagtcca ccggcaagca gacgtggagc gatctgcgtc agcgcaagaa gtccctgccg
    14281 gtggtcgccg cgctcgcggc cggcggttcc gcctccgagc ggctcgggga gatcctcgcc
    14341 gccgacgcca agagcagcga cttcgagacc ttctccgagg gggagttcgc ggcccgcgcc
    14401 gcgctgatcg aggaggcggg gggccgtgac tggaccgccg gggaggcgcg ccgccagcac
    14461 gccgtggccc tggacgccct gaacgccgtg gacatgtccg agcaggtgcg cgaccggttc
    14521 acggcgctcg ccgacttcgt cgtcgtacga aagagatgat ccctatcggt cgaatagccc
    14581 tcgcgtagtc gccggccggt gtgtgaggag agagcaccgg ccgacggcgg acccacagca
    14641 gacgcaaagc catgcacact gcacgaaggg gaagccatga cagcgacgac cgacggaagc
    14701 accggggctc tgccgccccg cgctgccgcg gccagcgaaa ccgacatcat catccccgtg
    14761 gcggccgggg tacaagaagc cgccacccgc gcgatgcaac gcgccaccga cttcctgctc
    14821 gccgggcagg acgccgaggg ctggtggaag ggcgacctcg agacgaacgt cacgatggac
    14881 gccgaggacc tgctgctccg tcagttcctg ggcatccgcg acgaggccac gacccgggcc
    14941 gccgcgctct tcgtccgggg cgagcagcgc gaggacggca cctgggccac cttctacggc
    15001 ggtccgcccg acctctccgc caccgtcgag gcgtacgtcg ccctgcggct ggcgggcgac
    15061 gcccccgagg cgccccacat gcgccgggcc tccgggtgga tccgcgagca ggggggcatc
    15121 gcctccgccc gggtcttcac ccggatctgg ctggccctgt tcggctggtg gaagtgggag
    15181 gacctgcccg aactcccgcc ggagctcctg ttcttcccca agtggttccc gctgagcatc
    15241 tacaacttcg gctgctgggc gcgccagacg atcgtgccgc tcaccgtggt gtcggcgaag
    15301 cgcccggtgc gtccggcgcc cttcccgctc gacgagctgc acaccgatcc cgcccgtccc
    15361 aacccggcca ggcccctcgc cccggtgaac agctgggacg ggctgttcca gcggctcgac
    15421 aaggtcgtac gcggctaccg gtcggtcgcg gtgcgcaggc tgcgcggcgc ggccctgaac
    15481 gccgccgccc gctggatcat cgaacggcag gagaacgacg gcgcctgggg cggcatccag
    15541 ccgccggccg tgtactcggt gatcgcgctg cacctgctcg gctacgacct ccggcacccg
    15601 gtgatgcggg ctggcctcga gtcgctggac cggtacgccg tgtggcgcga ggacggcgcg
    15661 cggatgatcg aggcctgtca gtcgccggtg tgggacacct gtctggcgac catcgcgctg
    15721 gccgacgcgg gacttcccgc cgaccatccc caactggtca aggccgccga ctggatgctc
    15781 ggcgaacagg tcgtccggcc gggcgactgg tcggtgagca ggcctcaact gccgccgggc
    15841 ggctgggcgt tcgagttcca caacgacaac tatcccgaca tcgacgacac cgccgaggtc
    15901 gtcctcgcgc tgcgccgggt caagcaccac gacccggagc ggatggaccg ggccatcgcg
    15961 cgcggcgtgc gctggaacct cgggatgcag tcgaagaacg gcggctgggg cgccttcgac
    16021 gtcgacaaca ccagcccgtt ccccaaccgg atgcccttct gcgacttcgg agaggtcatc
    16081 gacccgccgt ccgccgacgt caccgcgcac gtggtcgaga tgctggcggt cgaggggctc
    16141 tcgcacgacc cgcgtacccg gcggggcatc gagtggctgc tggccgaaca ggagccggac
    16201 ggctcgtggt tcgggcgctg gggcgtcaac tacgtctacg gcacagggtc ggtggtgccg
    16261 gccctcaccg cggcgggcct gcccgcgcag caccccgcga tccgacgggc cgtctcctgg
    16321 ctggagcggg tgcagaacga cgacggcggc tggggcgagg acctgcgctc ctaccaggac
    16381 gccacccgct ggagcggccg gggcgcctcc acggcctccc agaccgcctg ggcactgatg
    16441 gcgctgctgg cggccgggga gagggactcc aaggccgtcg agcggggcgt cgcctggctg
    16501 gcgcagaccc agcgggagga cggctcctgg gacgagccct acttcaccgg caccggcttc
    16561 ccctgggact tctcgatcaa ctaccacctc taccggcagg tcttcccgct gaccgcgctc
    16621 ggccggtacg tccacggaga gccgttcagc aagcagcccg ccacccacgc ggcttccgcc
    16681 gaggtcaagg ggagctgatg agcacacagt ccgtcccgtc gccgctgctg atcgcctgcg
    16741 ccctcggcat cgagcacctc gccctgcgca tgggcgagcg cggcggagcc gacgggccgg
    16801 tcaccgtcct gcggacgggc atggggccga aggccgcgga gcgctccgtc acccgcctcc
    16861 tggccgaccc gctgttcgcg ggcgcggccg tgctggccac gggtttctgt gccggcctcg
    16921 ccccgggcat gcaccccgga gacctggtcg tcgccgagga gacccgcgac ccgcgcggca
    16981 gtgtcccctg tgtcggcacc gagctgctcg tcaaggaact cgcccgcacc ctgcccgggc
    17041 ggaccgtcca caccggtccc ctcaccggct ccgaccacgt cgttcgcggt catgagcggg
    17101 ccgacctgct cgcgaccggc gcgatcgccc tcgacatgga atcggcggcc acgctgctga
    17161 gcgcagtgcg cgccgagccg cgcccggttg cggccgtccg ggtggtcgtg gacgctcctg
    17221 aacatgaact cgtccggatc ggcacggtac gcggtggaat atctgccttc cgcgtccttc
    17281 gctcggtcct gcctgctttt ttcgaatggc accgtaatgt tctgctcccc cggaggtgag
    17341 ccacatggcc atgccactgc gccagtccat caaggtcgct acatacctgg ccgaacagaa
    17401 gctccgcaag cgggagaagt ttccgctcat cgtcgagctg gaaccgctct tcgcctgcaa
    17461 cctcaagtgc gagggctgcg gcaagatcca gcacccggcg ggagtgctca agcagcgcat
    17521 gccggtcgcc caggccgtgg gcgcggtgct ggagtccggt gcgccgatgg tgtccatcgc
    17581 gggcggcgag cccctgatgc accctcagat cgacgagatc gtgcggcagt tggtggccaa
    17641 gaggaagtac gtcttcctct gcaccaacgc catgctgctg cgcaagaagc tggacaagtt
    17701 cacgccctct ccctacttcg ccttcgccgt gcacatcgac gggctgcgtg agcggcacga
    17761 cgagtcggtg gccaaggaag gcgtgttcga cgaggccgtg gcggccatca aggaggccaa
    17821 gcagcgcggc ttccgggtga ccaccaactc gaccttcttc aacaccgaca ccccgcagac
    17881 catcatcgag gtgctcaact acctcaacga cgacctcaag gtcgacgaga tgatgatctc
    17941 gcccgcctac gcctacgaga aggctcccga ccaggagcac ttcctcggcg tggagcagac
    18001 ccgcgagctg ttcaagaagg ccttctcggg tggcaaccgg cgccgctggc ggctcaacca
    18061 ctcaccgctg ttcctcgact tcctcgaggg caaggccgac ttcccgtgca cggcgtgggc
    18121 gatccccaac tactcgctgt tcggctggca gcggccctgc tacctgatga gcgacgggta
    18181 cgtgccgacg taccgcgagc tgatcgagga gacggactgg gacaagtacg gccgcggcaa
    18241 ggacccgcgc tgcgccaact gcatggcgca ctgcggctac gagcccaccg ccgtcctggc
    18301 caccatgggc tcgctgaagg agtccctgcg cgccctgcgc gagacagtca acggaaaccg
    18361 ggagtgacga catgaccgcg gtctccctgg gcgtccccgg gatgccggtc cggccggtgg
    18421 cgtcgcggcg tgagtcgcgg cggatccagg tcgggccggt ggcggtcggg ggcggggccc
    18481 cggtgtcggt gcagtcgatg acgacgacgc gtacgtcgga catcggcgcc accttgcagc
    18541 agatcgcgga actcaccgcc tccggctgcc agatcgtccg cgtcgcctgc cccacgcagg
    18601 acgacgcgga cgccctcgcg accatcgccc gcaagtccca gatcccggtg atcgcggaca
    18661 tccacttcca gccgaagtac gtgttcgccg cgatcgaggc gggctgcgcc gcggtccggg
    18721 tgaaccccgg caacatcaag cagttcgacg acaaggtgag ggagatcgcc aaggcggcga
    18781 aggagcacgg caccccgatc cgcatcggcg tcaacgccgg ctcgctggac cgccgcc
//
