LOCUS       NZ_LMEQ01000001        10405 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Streptomyces sp. Root1310 contig_1, whole genome shotgun sequence.
ACCESSION   NZ_LMEQ01000001
VERSION     NZ_LMEQ01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. Root1310
  ORGANISM  Streptomyces sp. Root1310
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2227698)
  AUTHORS   Bai,Y., Muller,D.B., Srinivas,G., Garrido-Oter,R., Potthoff,E.,
            Rott,M., Dombrowski,N., Munch,P.C., Spaepen,S., Remus-Emsermann,M.,
            Huttel,B., McHardy,A.C., Vorholt,J.A. and Schulze-Lefert,P.
  TITLE     Functional overlap of the Arabidopsis leaf and root microbiota
  JOURNAL   Nature 528 (7582), 364-369 (2015)
   PUBMED   26633631
REFERENCE   2  (bases 1 to 2227698)
  AUTHORS   Garrido-Oter,R. and Bai,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2015) Plant-Microbe Interactions, MPIPZ,
            Carl-von-Linne-Weg 10, Cologne 50829, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: A5 v. 20140604
            Assembly Name                     :: Root1310
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001426425.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:14:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,470
            CDSs (total)                      :: 8,385
            Genes (coding)                    :: 8,123
            CDSs (with protein)               :: 8,123
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 98 of 262
            Pseudo Genes (incomplete)         :: 219 of 262
            Pseudo Genes (internal stop)      :: 20 of 262
            Pseudo Genes (multiple problems)  :: 64 of 262
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:32:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 81923
            Orig. end                         :: 92328
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LMEQ01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(8..871)
                     /locus_tag="ASD48_RS00395"
                     /old_locus_tag="ASD48_00395"
     CDS             complement(8..871)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130459.1"
                     /locus_tag="ASD48_RS00395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00395"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_057599300.1"
                     /transl_table=11
                     /translation="MNTLPGRRTRVLAATTVTAGLMLVAACTSTDDGGSGSKTAAGGVE
                     LVKAGQLTTCTHLPYPPFQSEIDGKVQGFDVSLIDLAAKNLGVKQEILDTPFENFKTGA
                     FLNSGECDLAAAGMTITDERKKNVDFSDPYFEATQAVLVDKGAGVTSLADVKSKGVKLG
                     AQAQTTGEDYAKKQGFDPVSFESSDAVLNGLRTGQVKAVIIDFPVVQGWLKDKANADAF
                     KVVDNLNTGEQYGFTVKKGNTKLVAAINKALADAKADGTYKKLYEKWIGPYDESAASPS
                     AAASAS"
     misc_feature    complement(935..949)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
     gene            1056..2156
                     /locus_tag="ASD48_RS00400"
                     /old_locus_tag="ASD48_00400"
     CDS             1056..2156
                     /NRPS_PKS="Domain: Aminotran_5 (74-311). E-value: 3.6e-26.
                     Score: 83.9. Matches aSDomain:
                     nrpspksdomains_ASD48_RS00400_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="ASD48_RS00400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00400"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_057599301.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.2e-20, bitscore:
                     69.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLTAAHFASIEDRVARLLDTRQDVVIMQGEALLPLE
                     GAIRGTAGPGTVALNVITGPYGQTFGDWLRDCGATVIDLAVPFHTAVTAEQIREAFAEH
                     PGIDFVSLVHAEAATGNTNPVAAIGEVVREHGALFYLDAVASIGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPKAPRRSYLSLLDWKERWVDGGRKALLHAP
                     AQLEMLALEACVERIEAERPETVMARHALAAAATRAGALALGGGLEPYVRDAAEAAPVA
                     TTLRTPSGVVASELVARALASDPGLPLAAGGGALAGEMIRVNHYGGDATAGVVQGCLAA
                     LGAALAEKGLDVDPAGARRAAEGAWR"
     aSDomain        1278..1988
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS00400_Aminotran_5.1"
                     /evalue="3.60E-26"
                     /label="ASD48_RS00400_Aminotran_5.1"
                     /locus_tag="ASD48_RS00400"
                     /protein_end="311"
                     /protein_start="74"
                     /score="83.9"
                     /tool="antismash"
                     /translation="RDCGATVIDLAVPFHTAVTAEQIREAFAEHPGIDFVSLVHAEAAT
                     GNTNPVAAIGEVVREHGALFYLDAVASIGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWARMAANPKAPRRSYLSLLDWKERWVDGGRKALLHAPAQLEMLALEACVERI
                     EAERPETVMARHALAAAATRAGALALGGGLEPYVRDAAEAAPVATTLRTPSGVVASELV
                     ARALASDPGLPLAAG"
     gene            2306..2833
                     /locus_tag="ASD48_RS00405"
                     /old_locus_tag="ASD48_00405"
     CDS             2306..2833
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002247.1"
                     /locus_tag="ASD48_RS00405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00405"
                     /product="DinB family protein"
                     /protein_id="WP_057599302.1"
                     /transl_table=11
                     /translation="MTSDTPLILPDGRPVPLLTGDELPMLESWLAFHRATLELKCAGLD
                     DEQLRQPSAAPSELTLLGLVQHLAEVERNWFQRAFAGLDVPPVFEEGTGYALEAGRDLD
                     AALAAWRREIARGRELIAGRPLEATGRIADGPMAGLEVSLRWVLIHMIEEYARHNGHAD
                     ILRERIDGVTGA"
     misc_feature    complement(2973..2988)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
     gene            3116..3622
                     /gene="ectA"
                     /locus_tag="ASD48_RS00410"
                     /old_locus_tag="ASD48_00410"
     CDS             3116..3622
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121394.1"
                     /locus_tag="ASD48_RS00410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00410"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_057599303.1"
                     /transl_table=11
                     /translation="MTAAQADLRIDSPSVEDGAALWRLAKESGTLDLNSSYSYLLWCRD
                     FAATSAVARAENGEPVGFVTGYVRPEDPRTLLVWQVAVDAAHRGRGLAAALLDGLTARV
                     AAERPLTAVETTITPGNTASERLFTSFADRHGATVTREVLFGAGLFPDGPHDPEVLYRI
                     GPLAF"
     gene            3684..4955
                     /gene="ectB"
                     /locus_tag="ASD48_RS00415"
                     /old_locus_tag="ASD48_00415"
     CDS             3684..4955
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 2e-73.
                     Score: 239.3. Matches aSDomain:
                     nrpspksdomains_ASD48_RS00415_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="ASD48_RS00415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00415"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_057599304.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.2e-79, bitscore:
                     262.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRSWPAVFDRAQGSRMYDEDGHRY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLMRDGVTHGLDMSTVAKRTFLQTFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYFEGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALKDLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATAALETYWADGSAMEKQTRTRGEQIEQGL
                     ISITEENLADVKEYRGRGLVWGLEFHEKARAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPEELDEGLSVIARAVRETV"
     aSDomain        3768..4781
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS00415_Aminotran_3.1"
                     /evalue="2.00E-73"
                     /label="ASD48_RS00415_Aminotran_3.1"
                     /locus_tag="ASD48_RS00415"
                     /protein_end="366"
                     /protein_start="28"
                     /score="239.3"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHRYLDFFAGAGSLNYGHNNPVLKRALIDYLM
                     RDGVTHGLDMSTVAKRTFLQTFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYFEGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALKDLCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALETYWADGSAMEKQTRTRGEQIEQGLISITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="ASD48_RS00420"
                     /old_locus_tag="ASD48_00420"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="ASD48_RS00420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00420"
                     /product="ectoine synthase"
                     /protein_id="WP_057599305.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.4e-54, bitscore:
                     178.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVRAASGTWESKRIVLAQERVGFSLHETILY
                     AGTETSMWYANHIEAVVCVEGAAELTDHETGRTHSITPGTMYLLDGHERHTLRVKEDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     misc_feature    complement(5410..5425)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            5411..6298
                     /gene="thpD"
                     /locus_tag="ASD48_RS00425"
                     /old_locus_tag="ASD48_00425"
     CDS             5411..6298
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436210.1"
                     /locus_tag="ASD48_RS00425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00425"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_057599306.1"
                     /sec_met_domain="PhyH (E-value: 3.2e-38, bitscore: 129.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLTDLYPTRGTTEVSVPRKDPVVWGSPDAPGPIPAAGLQEYER
                     DGFLAVDRLISDDEVTVCRRELERLVADPEIRADGRSIVEPKSKEIRSVFEVHKLSPVF
                     AALVRDERVVGRARQILGSDVYVHQSRINVKPGFGAGGFYWHSDFETWHAEDGLPNMRT
                     VSVSIALTENLDTNGGLMIMPGSHRTFLGCAGATPEDNYKKSLQMQDAGTPSDEALTAM
                     AGDYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFAAPVR
                     RPEYIGARDFTPVR"
     gene            complement(6418..7470)
                     /locus_tag="ASD48_RS00430"
                     /old_locus_tag="ASD48_00430"
     CDS             complement(6418..7470)
                     /NRPS_PKS="Domain: Aminotran_5 (17-344). E-value: 2.3e-22.
                     Score: 71.3. Matches aSDomain:
                     nrpspksdomains_ASD48_RS00430_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130466.1"
                     /locus_tag="ASD48_RS00430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00430"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_057599307.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.9e-17, bitscore:
                     59.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFTPKTTYLNTASNGLLPARTVAALHEAAVLRAEG
                     RPLTPLHEDVEACRAAYARLAGVPVTRVALGASVAAHTGVIAASLPAGAEVLTAEDDFT
                     SVLNPFHVRGDLKVRAVPLERIAESVRPGTALVAVSAAQSADGRIADLPALREAARTHG
                     ARTYVDFSQSAGWLATDADAHDFSAAVSFKWLLGPHGAAFLVVPEDFGGLSPVLAGWVA
                     GERPWDSCYGPVAELARSARRFDLTHALFTYAGLRRSLELVEELGVAAIGAHDLALADR
                     FRAGLAGLGHRPVAAPGSAIVSVPGLGFRQPELAAAGIQVSDRSGHLRASFHLFNTPAD
                     VDRLLDALAA"
     aSDomain        complement(6439..7419)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASD48_RS00430_Aminotran_5.1"
                     /evalue="2.30E-22"
                     /label="ASD48_RS00430_Aminotran_5.1"
                     /locus_tag="ASD48_RS00430"
                     /protein_end="344"
                     /protein_start="17"
                     /score="71.3"
                     /tool="antismash"
                     /translation="YLNTASNGLLPARTVAALHEAAVLRAEGRPLTPLHEDVEACRAAY
                     ARLAGVPVTRVALGASVAAHTGVIAASLPAGAEVLTAEDDFTSVLNPFHVRGDLKVRAV
                     PLERIAESVRPGTALVAVSAAQSADGRIADLPALREAARTHGARTYVDFSQSAGWLATD
                     ADAHDFSAAVSFKWLLGPHGAAFLVVPEDFGGLSPVLAGWVAGERPWDSCYGPVAELAR
                     SARRFDLTHALFTYAGLRRSLELVEELGVAAIGAHDLALADRFRAGLAGLGHRPVAAPG
                     SAIVSVPGLGFRQPELAAAGIQVSDRSGHLRASFHLFNTPADVDRL"
     misc_feature    complement(7507..7523)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.76"
                     /tool="antismash"
     gene            complement(7567..8283)
                     /locus_tag="ASD48_RS00435"
                     /old_locus_tag="ASD48_00435"
     CDS             complement(7567..8283)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130467.1"
                     /locus_tag="ASD48_RS00435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00435"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_057599308.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDQVEVVHRSFELDP
                     GRAKGDVQPVLAMLTKKYGMSEAQAQSGEENLGAQAAAEGLDYRTRDRDHGNTFDMHRL
                     LHFAKEHGRQDELIQLLYRANFAEERSVFSEGDERLVELAAEAGLDAEAARAVLADPAR
                     YADEVRADEREAAQLGANGVPFFVLDRAYGVSGAQPAEVFSQALTQAWGDRSPLKLIDD
                     GGAQACGPDGCAVPQQ"
     misc_feature    complement(8363..8376)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.0"
                     /tool="antismash"
     gene            complement(8444..8959)
                     /locus_tag="ASD48_RS00440"
                     /old_locus_tag="ASD48_00440"
     CDS             complement(8444..8959)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130468.1"
                     /locus_tag="ASD48_RS00440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00440"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_199875602.1"
                     /transl_table=11
                     /translation="MIEERVRGNRVIRTAVPAEAERVAALHARARATYYPDGVPQDGTD
                     WLAAWRAAIERPDGHVLCVVEQGRLVGLASFRTPEGAPAHTVKLFQFHVDPDHWRSGIG
                     TDLHTACVEQWRADGRRTAVLDVHVDNRRAQAFYARQGWSPDPENPPGEGEHHLWLRYA
                     VTRERPGE"
     gene            complement(9051..9659)
                     /locus_tag="ASD48_RS00445"
                     /old_locus_tag="ASD48_00445"
     CDS             complement(9051..9659)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="ASD48_RS00445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ASD48_00445"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_057599309.1"
                     /transl_table=11
                     /translation="MDVSLPELPFPLRTYGPDGHWSHENGVLTGWAGARQDRFVPPTGD
                     GVESAADAPRLLGAPEGDFQLIARVTVGFNWAFDAGVLYVHVGERAWAKLCLEYSPDVA
                     TVCTVVTRGHSDDANSFTVEGSSVWLRVSRTGRAFAFHASRDGKRWTFVRLFTLGEEKE
                     TGAALVGFMTQSPMGEGCVVTYDHLEFRTEWPDDLRDGS"
ORIGIN
        1 catggggtca ggaggccgag gcggcggcgg acggggacgc ggccgactcg tcgtacggcc
       61 cgatccactt ctcgtacagc ttcttgtagg tgccgtcggc cttcgcgtcc gccagcgcct
      121 tgttgatcgc ggcgaccagc ttggtgttgc ccttcttcac cgtgaagccg tactgctcac
      181 cggtgttgag gttgtcgacg accttgaagg cgtcggcgtt ggccttgtcc ttcagccagc
      241 cctggacgac cgggaagtcg atgatgacgg ccttgacctg gccggtgcgc agaccgttga
      301 ggacggcgtc ggaggactcg aaggagaccg ggtcgaagcc ctgcttcttg gcgtagtcct
      361 cgccggtggt ctgcgcctgg gcgccgagct tgacgccctt ggacttcacg tcggcgagcg
      421 aggtgacacc cgcgcccttg tcgaccagga cggcctgggt ggcctcgaag tacgggtcgg
      481 agaagtcgac gttcttcttg cgctcgtcgg tgatggtcat gccggccgcg gccagatcgc
      541 actcgccgga gttcaggaag gcgccggtct tgaagttctc gaacggcgtg tcgaggatct
      601 cctgcttcac gcccaggttc ttggcggcca ggtcgatcag ggacacgtcg aagccctgca
      661 ccttgccgtc gatctccgac tggaagggcg ggtagggcag gtgggtgcag gtggtgagct
      721 ggcccgcctt gacgagctcg accccgccgg cggcggtctt ggagccgctg ccgccgtcat
      781 cggtggaggt gcaggcggcg acgagcatca gcccggccgt cacggtggtg gcggccagga
      841 cgcgggtccg gcggccgggg agggtgttca cggcgggcct tcctgtaagg gaactgtggg
      901 ttccgattat aaggagatgt ttgaggctct caaatcaaac cgatggtcac cgggccgcgc
      961 gacctgcgaa gtgaccggtg gggagggcgg gaggggtggg ggggcgtgcc ggttagtctc
     1021 gtcacctcta cccagtgagt gaagagagca ccgccgtgac acacccgttc ctggatcttg
     1081 ccccgctgac cgccgcacat ttcgcctcga tcgaggaccg cgtggcgcgg ctgctggaca
     1141 cccggcagga cgtcgtgatc atgcagggcg aggcgctgct gccgctggag ggcgcgatcc
     1201 gcggcacggc cggtccgggc acggtcgcac tgaacgtgat caccggtccg tacgggcaga
     1261 ccttcgggga ctggctgcgg gactgcggcg ccacggtgat cgatctggcc gtgcccttcc
     1321 acacggcggt gacggccgag cagatccggg aggcgttcgc ggagcacccc gggatcgact
     1381 tcgtgtcgct ggtgcacgcg gaggcggcga ccggcaacac caacccggtc gcggcgatcg
     1441 gcgaggtggt gcgggagcac ggcgcgctgt tctacctgga cgcggtcgcc tcgatcggcg
     1501 ccgagccggt gctgccggac gcgtggggcg tggacctgtg cgtgatcggc gcgcagaagg
     1561 cgatgggcgg cccggcgggc gtgtcggcgg tgtcggtgag cgagcgggcg tgggcgcgga
     1621 tggccgcgaa cccgaaggcg ccgcgccggt cgtacctgtc cctgctggac tggaaggagc
     1681 gctgggtcga cggcggtcgc aaggcgctgc tgcacgcgcc ggcgcagctg gagatgctgg
     1741 cgctggaggc gtgtgtcgag cggatcgagg cggagcggcc ggagacggtg atggcccggc
     1801 acgcgttggc cgccgccgcg acgcgcgcgg gcgcgctcgc gctgggcggc gggctggagc
     1861 cgtacgtgcg cgacgcggcg gaggcggcgc ccgtcgccac gacgctgcgg acgccgtcgg
     1921 gggtggtggc gtcggagctg gtggcgcggg cgctggcgag cgaccccggg ctgccgctgg
     1981 ccgcgggcgg gggcgcgctg gccggggaga tgatccgggt caaccactat gggggcgacg
     2041 cgaccgccgg cgtggtgcag ggatgcctgg cggcgctggg tgccgcgctg gcggagaagg
     2101 ggctggacgt cgatccggca ggggcccggc gggccgcgga gggcgcctgg cggtaggcgc
     2161 cgccgggccg gatcaccggc cgtcggatca ccgtcggccg gaccggcgtc ggccggaccg
     2221 gcgtcggccg gaccggcgtc ggccggaccg gcgtcggccg gaccggcgac atcccgctgt
     2281 cggtgccgcc tgccatgctg cccccatgac ctccgacact cccctcatcc tccccgacgg
     2341 ccggcccgtc cccctgctga ccggtgacga actccccatg ctggagagct ggttggcatt
     2401 ccaccgggcc acgctcgagc tgaagtgcgc ggggctggac gacgagcagc tgcggcaacc
     2461 ctcggcagcg ccctccgagt tgaccctgct cggtctcgtc cagcatctcg cggaggtgga
     2521 acgcaactgg ttccagcggg cgttcgccgg gctggacgtg ccgcccgtct tcgaggaggg
     2581 gaccgggtac gccctcgagg ccgggcggga cctcgacgcg gcgctggccg cctggcggcg
     2641 ggagatcgcg cgcggacgtg agctgatcgc cgggcggccg ctcgaggcca ccgggcggat
     2701 cgccgacggg ccgatggccg ggctcgaggt cagcctgcgc tgggtgctca tccacatgat
     2761 cgaggagtac gcccggcaca acggccacgc ggacattctc cgggagcgta tcgacggggt
     2821 taccggagcc tgaattcaag gtaattcacg aattcccgtc ggcccctttc ccgtcccctt
     2881 ctcgctcccc tttctcgacg ataggtttcc tgagttcttc tgccccgatt ccccgcacct
     2941 aaacgcgggg aatttcaggc aggtttcgag tgcgtgacgc attgcacaag cgaggcgata
     3001 tgcccttttc gcgcggtgca aaagcggacg aaacgtccat ctgtcccggc ggcatcacgg
     3061 aatcaccacg gcctctccat gcgttattga atttccctgg gtaagttcaa tccgcatgac
     3121 tgccgcacaa gcagacctac gtatcgacag cccgtcggtg gaagacggcg cagcgctctg
     3181 gcgtctcgcg aaggaatccg gaaccctcga cctgaactcg tcctacagct atctgctgtg
     3241 gtgtcgcgac ttcgccgcca cctcggcggt ggcccgcgcg gagaacggcg agccggtcgg
     3301 gttcgtcacc gggtacgtcc ggccggagga cccgcggacc ctgctggtct ggcaggtggc
     3361 cgtcgacgcc gcgcaccgtg gtcgcggcct cgccgccgcc ctgctggacg ggctgaccgc
     3421 acgggtcgcc gccgaacgcc cgctcacggc cgtggagacc acgatcacgc cgggcaacac
     3481 cgcctccgag cggctgttca cctcgttcgc cgaccggcac ggggcgaccg tcacccgtga
     3541 ggtgctgttc ggcgccggtc tgttccccga cggcccgcac gaccccgagg tcctgtaccg
     3601 gatcggcccc ctcgccttct gacccgcgcg ccccgcgtcc gcccgcctct ccttccgacc
     3661 cccactccga ggagcgtttc gtcgtgacca tcacccagcc cgacctcagc gtgttcgaga
     3721 ccctcgagtc cgaggtgcgc agctactgcc gcagctggcc cgccgtcttc gaccgcgcgc
     3781 agggcagccg gatgtacgac gaggacggtc accgctacct ggacttcttc gccggtgccg
     3841 gatccctcaa ctacggccac aacaaccctg tgctgaaacg ggcgttgatc gactacctga
     3901 tgcgggacgg cgtcacgcac gggctcgaca tgtcgaccgt ggccaagcgg accttcctgc
     3961 agaccttcca ggatctggtg ctgcggccgc gtgacctgcc ctacaaggtc atgttcccgg
     4021 gcccgaccgg caccaacgcc gtggagtcgg ccctgaagct ggcgcggaag gtgaagggac
     4081 gcgaggccat cgtctcgttc accaacgcct tccacgggat gtccctcggg tcgctcgccg
     4141 tgaccggcaa cgccttcaag cgggccggtg ccggggtacc gctggtgcac ggtacgccca
     4201 tgccgttcga caactacttc gagggccagg tacccgactt cctctggttc gagcggctgc
     4261 tcgaggacca gggatccggg ctcaacaagc cggccgccgt gatcgtcgag accgtgcagg
     4321 gcgagggcgg catcaacgtc gcgcgtccgg agtggctgcg ggcgctcaag gacctgtgcg
     4381 aacgccagga catgctgctg atcgtcgacg acatccagat gggatgcggg cgcaccgggg
     4441 ccttcttctc cttcgaggag gccgggatcg tccccgacat cgtgaccgtc tccaagtcga
     4501 tcagcggcta cggactgccc atgtcgctct gcctgttcaa gcccgagctg gacgtgtggg
     4561 agccgggtga gcacaacggc accttccgcg gcaacaaccc ggccttcgtc accgccaccg
     4621 ccgccctgga gacgtactgg gccgacgggt ccgcgatgga gaagcagacc cggacgcggg
     4681 gcgaacagat cgagcagggg ctcatctcga tcaccgagga gaacctcgcc gacgtcaagg
     4741 agtaccgggg ccgcggcctg gtgtggggcc tggagttcca cgagaaggcg cgggccgggc
     4801 gggtcgcgaa gcgggccttc gagctcgggc tgctgatcga gacgtccggt ccggagagcg
     4861 aggtcgtcaa actgctgccg gccctgacca tcacccccga ggaactggac gagggcctca
     4921 gtgtcatcgc ccgcgccgta cgggagaccg tctgacgtcc tcggaccccg tatcccctac
     4981 ctaggaggca tcgcaccacc gtgatcgtcc gttcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcatgtgcg ggcggcgtcc ggcacctggg agagcaagcg catcgtgctc gctcaggaac
     5101 gggtcgggtt ctcgctgcac gaaacgattc tgtacgcggg taccgagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcgtctgcg tcgagggcgc ggccgaactg accgaccacg
     5221 agaccgggcg gacgcacagc atcacgcccg gcaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct gcgcgtcaag gaggacttcc gctgcatctg cgtgttcaac ccgcccgtca
     5341 cggggcggga ggaccacgac gagaacggcg tgtaccccct gctgaccgag cccgaggagg
     5401 tgtaagcatc gtgaccaccc tcaccgatct ctaccccacc cgtggcacca ccgaggtgtc
     5461 cgtaccccgc aaggaccccg tcgtctgggg ctcccccgac gcgccgggcc ccatcccggc
     5521 ggccggcctc caggagtacg agcgcgacgg cttcctcgcc gtcgaccggt tgatcagcga
     5581 cgacgaggtc accgtctgcc gacgggagct ggagcggctg gtcgccgacc cggagatccg
     5641 ggccgacggg cgctcgatcg tcgagccgaa gtccaaggag atccgctccg tcttcgaggt
     5701 gcacaagctc agtcccgtgt tcgccgcgct cgtccgcgac gagcgggtcg tggggcgcgc
     5761 ccggcagatc ctgggctcgg acgtgtacgt gcaccagtcg cggatcaacg tcaagccggg
     5821 cttcggggcc gggggcttct actggcactc cgacttcgag acctggcacg ccgaggacgg
     5881 tctgccgaac atgcggacgg tgtccgtgtc gatcgcgctg accgagaacc tcgacaccaa
     5941 cggcgggctc atgatcatgc cgggctcgca ccggacgttc ctcggctgcg ccggggcgac
     6001 gcccgaggac aactacaaga agtcgctcca gatgcaggac gcgggcaccc cctcggacga
     6061 ggcgctgacc gcgatggccg gcgactacgg catcaagttg ttcacgggca aggccggttc
     6121 ggcgacctgg ttcgactgca actgcatgca cgggtccggc gacaacatca cgccgttccc
     6181 gcgcagcaac gtgttcatcg tgttcaacag cgtggagaac acggccgtgg agccgttcgc
     6241 ggcaccggtc cgccggccgg agtacatcgg cgcgcgggac ttcacgccgg tgcgctgaac
     6301 cggttcccgc aggctgcgcc ggcaggcccg gacgccccca cccggatcgg gtggcggcgt
     6361 ccgggcctgc cgtgcgtcgg ggccgccgtg cttccgggcc tgccgcgcgt cggatcctca
     6421 ggccgcgagg gcgtcgagca gccggtcgac gtccgcgggc gtgttgaaca ggtggaagga
     6481 agcgcgcaga tggcccgagc gatcggagac ctggatcccg gcggcggcca gctcgggctg
     6541 acggaagccg agtccgggca ccgagacgat cgcggagccg ggcgccgcga cgggccggtg
     6601 gcccagcccg gcgagtcccg cgcggaagcg gtcggccagg gcgaggtcgt gggcgccgat
     6661 cgccgccacg ccgagttcct ccaccagttc gagggaacgg cgcagtccgg cgtaggtgaa
     6721 cagggcgtgc gtcaggtcga accgccgggc cgagcgggcg agttcggcca ccggtccgta
     6781 gcagctgtcc cagggccgct cgccggccac ccatccggcg aggaccggtg acagcccgcc
     6841 gaagtcctcc ggtaccacca ggaaggccgc accgtgcggg ccgagcagcc acttgaagga
     6901 gacggcggcg gagaagtcat gggcgtcggc gtccgtcgca agccagccgg ccgactgcga
     6961 gaagtcgacg taggtacggg cgccgtgcgt cctggccgcc tcgcgcaggg cggggaggtc
     7021 ggcgatccgg ccgtcggcgg actgggcggc gctcaccgcg accagtgccg tgccggggcg
     7081 gacggactcg gcgatccgct ccagcgggac ggcgcggacc ttgaggtccc cgcggacgtg
     7141 gaacgggttg agtacggagg tgaagtcgtc ctcggcggtg agcacttccg cgcccgcggg
     7201 gagcgaggcg gcgatcacgc cggtgtgcgc ggcgaccgag gcgcccagcg ccacccgggt
     7261 gaccgggacg ccggccagcc gggcgtacgc ggcccggcag gcctccacgt cctcgtgcag
     7321 cggggtgagc ggcctgccct cggcgcgcag caccgccgcc tcgtgcaggg cggcgacggt
     7381 acgggccggc agcaggccgt tgctcgccgt gttcaggtac gtggtcttcg gggtgaactc
     7441 ggcgcggacg aggctctcga aggtctccat gcgtccactg tgggaccccg ggaccccctc
     7501 gtccattgcc gatttttacg tgattccact aaggagagct tatagatcgg ctctcacctg
     7561 cgggtttcac tgctgcggga ccgcgcaccc gtccgggccg caggcctgtg cgccgccgtc
     7621 gtcgatcagc ttcagcgggg aacggtcgcc ccaggcctgc gtgagggcct ggctgaagac
     7681 ctctgcgggc tgggccccgg agacaccgta ggcgcggtcg aggacgaaga agggcacgcc
     7741 gttcgccccg agctgagcgg cctcgcgctc gtcggcgcgg acctcgtcgg cgtagcgggc
     7801 cgggtcggcg aggaccgcgc gggcggcctc ggcgtccaga ccggcctcgg cggccagttc
     7861 cacgagccgc tcgtcgccct cggagaagac ggaccgctcc tcggcgaagt tcgcccggta
     7921 gaggagctgg atcagctcgt cctgccggcc gtgttccttg gcgaagtgca gcaggcggtg
     7981 catgtcgaag gtgttgccgt ggtcgcggtc ccgggtgcgg tagtcgaggc cctcggcggc
     8041 ggcctgggcg cccaggttct cctcgccgga ctgcgcctgc gcctcgctca tcccgtactt
     8101 cttggtgagc atggccagta cgggctggac gtcgcccttc gcgcggcccg ggtccagctc
     8161 gaaggagcgg tgcaccacct cgacctggtc acggtgcggg aaggccgcga gcgccttctc
     8221 gaagcgggcc ttgcccacgt agcaccaggg gcaggcgatg tcgctccaga tctcgacgcg
     8281 catgtctcgg ctctctccag gtcgtgcggc tgggtacgga gctcccccgc gggagatccg
     8341 cacggaggtt ccctccggcg agtgcatgaa cgttcaagca ggacgtttca ttccccggcc
     8401 gctccccggg ctgctcgcgg tcactccccc cgccgctccc cggtcactcc cccggccgct
     8461 ccctggtcac ggcgtacctc agccagaggt ggtgctcgcc ctcgccgggc gggttctccg
     8521 ggtccgggct ccagccctgc cgggcgtaga acgcctgggc ccgccggttg tcgacgtgca
     8581 cgtcgagcac ggccgtgcgc cgcccgtccg cgcgccactg ctcgacgcag gccgtgtgca
     8641 ggtccgtacc gatccccgaa cgccagtggt cggggtcgac gtggaactgg aacagcttga
     8701 ccgtgtgcgc gggcgcgccc tccggcgtgc ggaaggaggc cagaccgacg agccgcccct
     8761 gctcgacgac gcacagcaca tggccgtcgg gccgctcgat ggccgcgcgc caggcggcga
     8821 gccagtcggt gccgtcctgc gggaccccgt cggggtagta cgtggcccgg gcgcgcgcgt
     8881 gcagcgcggc gaccctctcc gcctcggcgg gaacggcggt gcggatcacc cggtttcccc
     8941 tgacacgttc ttcgatcatg caggtgagga cgaaagggct ctcgcgccgg ttccggccgg
     9001 ctagtgctgc gaccggaaag gttcaccggc tcgcgacgcc cggcacggca ctagcttccg
     9061 tcccgcaggt cgtccggcca ctcggtcctg aactcgagat ggtcgtaggt cacgacgcac
     9121 ccctcgccca tcggtgactg cgtcatgaag ccgaccaggg ccgcgccggt ctccttctcc
     9181 tcgcccagtg tgaacaggcg gacgaaggtc cacctcttgc cgtcccggga ggcgtggaag
     9241 gcgaaggcac ggccggtcct gctcacccgg agccagaccg aactgccctc cacggtgaag
     9301 gagttggcgt cgtcggagtg tcctcgggtg accaccgtgc agacggtggc cacgtccggg
     9361 gagtactcca ggcacagctt ggcccaggcc cgctcaccga cgtggacgta caacacgccc
     9421 gcgtcgaagg cccagttgaa tccgacggtc acgcgggcga tgagctggaa gtcaccctcc
     9481 ggcgccccca gcagccgggg cgcgtcggcg gcggattcca ccccgtcgcc ggtgggcggc
     9541 acgaaccggt cctgccgggc cccggcccac ccggtgagca ccccgttctc gtgggaccag
     9601 tgcccgtcgg gcccgtaagt acgcagggga aagggcagtt cgggaagcga gacgtccatg
     9661 acctcatcat ctcagggagc ggagaaaccg agaggcgcgg gggacgggcg cgacaagcca
     9721 ccggaaggct gcgcccgatg atccaccgcg cacggtccac cgcgccactc gacgccgccc
     9781 gcgcggagcg ctaccgctcc agacgcccgt tgaaccggcg aggcagcccc agctcgttct
     9841 cgtcccgcag ctccacgggc agcagcgccg cgggagcgtt ctggtaggcg accggccgca
     9901 gccaccgctc gatggcggtc ccgccgaccg aggtggacgt ggaggtggtg gccgggtacg
     9961 ggccgccgtg atgctgggcc ggcgcgacgg cgacgcccgt gggccagccg ttcaccagca
    10021 cgcgcccggc cagcggggtc agctccgcga ggatctcgcc gcccctgccc tcgcccgcgg
    10081 cctctccctc ggacagctgg acggtcgccg tgaggtttcc gggcaggcgc gacagcaccg
    10141 ccttgacctc gccctcgtcg tcgtagcgga ccacgacggt gaccggcccg aagcactctt
    10201 cgagcagcag gtcgtgcgcg ccctcggcgg ccagccggga ggccggcacg gtcaggaagc
    10261 ccgcgctgac ggtgtgctcc ccgccggcgc cgggcgtcac cggcgactcc acgtcgggca
    10321 gctcggcccg ctcggcgacc ccggcgatga agttgtcgcg catccggtgg tccagcagca
    10381 cgcccgcgtc ggtgtcgctc accgc
//
