LOCUS       NZ_JXCW01000001        12151 bp    DNA     linear   CON 25-MAY-2024
DEFINITION  Apilactobacillus kunkeei strain LAce contig001, whole genome shotgun
            sequence.
ACCESSION   NZ_JXCW01000001
VERSION     NZ_JXCW01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Apilactobacillus kunkeei
  ORGANISM  Apilactobacillus kunkeei
            Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
            Apilactobacillus.
REFERENCE   1  (bases 1 to 366741)
  AUTHORS   Tamarit,D., Ellegaard,K.M., Wikander,J., Olofsson,T., Vasquez,A. and
            Andersson,S.G.
  TITLE     Functionally Structured Genomes in Lactobacillus kunkeei Colonizing
            the Honey Crop and Food Products of Honeybees and Stingless Bees
  JOURNAL   Genome Biol Evol 7 (6), 1455-1473 (2015)
   PUBMED   25953738
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366741)
  AUTHORS   Tamarit,D., Ellegaard,K.M., Wikander,J., Olofsson,T., Vasquez,A. and
            Andersson,S.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2015) Institute of Cell and Molecular Biology,
            Husargatan 3, Uppsala 75236, Sweden
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2012
            Assembly Method                   :: Velvet v. 1.1.06
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001421115.1-RS_2024_05_25
            Annotation Date                   :: 05/25/2024 01:02:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,420
            CDSs (total)                      :: 1,365
            Genes (coding)                    :: 1,362
            CDSs (with protein)               :: 1,362
            Genes (RNA)                       :: 55
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3, 1 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:58:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 54171
            Orig. end                         :: 66322
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JXCW01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12151
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12151
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12151
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7151](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7151)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    364..380
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.26"
                     /tool="antismash"
     gene            complement(934..2607)
                     /gene="ade"
                     /locus_tag="RZ55_RS00245"
                     /old_locus_tag="RZ55_05130"
     CDS             complement(934..2607)
                     /EC_number="3.5.4.2"
                     /GO_function="GO:0000034 - adenine deaminase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /gene="ade"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010021817.1"
                     /locus_tag="RZ55_RS00245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RZ55_05130"
                     /product="adenine deaminase"
                     /protein_id="WP_057175420.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 8.8e-38, bitscore:
                     124.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTIDLLVQNGKVLNVFTNRFEINDIFINNGKIIAVGNHPELKAK
                     EIIDAKNQFVVPGFIDSHVHIESSLVTPSELGKVLVPMGVTSIVVDPHEIANVSGSDGI
                     KYMIEDAKQTPLEVLVMLPSSVPATSFEHNGATLTAKDLHLLYNEESVIGLAEVMDYPA
                     VHNKDKDMMAKLNDALDHGYQIDGHGAGLSLEQLKEYRENGITTDHESVSLDQMNDRLA
                     ADMNVFLREGTVERDLQNTIQAVTEDNSDRFSFCTDDKFITTIIREGSINFNIKLSIQY
                     GLKPETAYKIASYNAAVAHKLDKLGAIKEDYQADLVIVDHLDQVDVKTIIKNGKVMTED
                     SFNTTPIKFGDNCVHQNLSVDDLKLPLNSNKCHVMGVIANHIETTHSIQEVPIEDGYFK
                     TDLENDILLMVDAERHHQLGTYGLALVHGFKIKNGAIATTVAHDSHNIVAVGTTKEAIY
                     RAIQEVTATKGGIAVVDEEKTLATMPLPVAGLMSDKSWQAASHELEEIELAFKEISDGV
                     DFDPFITLSFLTLPVIPTLKLTDQGLYDFDQQKIISVEAE"
     gene            2846..4405
                     /locus_tag="RZ55_RS00250"
                     /old_locus_tag="RZ55_05140"
     CDS             2846..4405
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011667019.1"
                     /locus_tag="RZ55_RS00250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RZ55_05140"
                     /product="Nramp family divalent metal transporter"
                     /protein_id="WP_057175421.1"
                     /transl_table=11
                     /translation="MSENVSHEKKGFVEYANGPSLEEINGGIKVPKDWGFWKTLFAYSG
                     PGALVAVGYMDPGNWATSITGGQDFGYLLMSVILISSLIAMLLQYMAAKLGIVSQMDLA
                     QAIRSRTSKTLGVVLWIMTELAIMATDIAEVIGGAIALYLLFHIPLILAVFLTVFDVLL
                     LLLLTKVGFRKIEAIVVCLILVILVVFIYEVVLSKPDWLGAFKGLIPVERTFSPNGPVN
                     GLTPLKGALGIIGATVMPHNLYLHSAIAQTRAIDHDDEEDVARTVRFATWDSNIQLTFA
                     FFVNALLLIMGVAVFKTGAVQDSSFFGLFDALNNTKMLSNPILIGVAKSGILSTLFAVA
                     LLASGQNSTITGTLSGQVIMEGFVNLKVPLWARRLITRLISVIPVLICVGLTAGESTVQ
                     QHAAINDLIENSQIFLAFALPFSMLPLLMMTDSKEEMGSRFKNTFWVKILGWISVLVLI
                     FLNGYNMPDAVVGFISDNPTAAQNAHAAVIAYAINAVILALLIWTLVELYLGNKRLRNR
                     EN"
     gene            4447..4884
                     /locus_tag="RZ55_RS00255"
                     /old_locus_tag="RZ55_05150"
     CDS             4447..4884
                     /GO_function="GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270459.1"
                     /locus_tag="RZ55_RS00255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RZ55_05150"
                     /product="universal stress protein"
                     /protein_id="WP_143444043.1"
                     /transl_table=11
                     /translation="MDVEINFKNILVAVDDSDDALLAFKYAISRAKSEGAKLTIVSVLE
                     DEEINVFQSLSGQFVHGKYEDLEKHLEKYKKTAEEAGVADVNVIASTGEAGETIIKDVL
                     PKVNPDLLIVGSESKKGLARHFGSQAAYMAKYSPVSVMVIR"
     gene            complement(5001..7151)
                     /locus_tag="RZ55_RS00260"
                     /old_locus_tag="RZ55_05160"
     CDS             complement(5001..7151)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723786.1"
                     /locus_tag="RZ55_RS00260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RZ55_05160"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_057175422.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.8e-40, bitscore:
                     130.8, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 3.8e-288, bitscore:
                     951.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISRYYVPQMDESDCGVAALATVLRKYNSYCSLSSLRDLSKTGVN
                     GTTALGLIKAAKHFNLETKAIKADLSLFKEKNLNYPIIVQVLKDNRFYHFYVVFEKKNN
                     YIKIADPDPNVGIIKMNINTFVDEWTGIALLFHPNEKYIPIKHTGKSIWNYFKYIFKYK
                     KLVSEIISSSLCITLIGIIGSYYIEILVDKLIPNKNFQLINIFSLCLIFSYVIQQTMSF
                     IQNYLVSILNRKLSIDIILKYINHIFNLPMSFFSSRKTGEIVSRFNDANKIIDALANTI
                     ISFFLDTFIILILSLFLIIQSYTLFLVTLLLIPAYSLIIYIFFKPFKKLNNQVMESNSK
                     LSSSIIDDLKGIETIKSLNSENDCYKKIKDEFNNYLEKTLKYSRLIFMQESLKTSIKLI
                     MNVAILWLGSELVIRNKLSIGQLMAFSALISYFISPIENIISLQTKIQSAQVSNNRLNE
                     VMTVKSEFKEKRKIDNFKELDGKVTFNNVFYSYGYDKKVLAGVNLSIYKNQKISIVGIS
                     GSGKSTIAHLLNGFYNAEYGNIEINGHNIKEINRKTLRSYINYVPQTPYLLNGTFLDNI
                     LLGCRDGLNYNDIKEACDISEINDLIESTPLGFKTNISENGVVLSGGEKQRITIARALL
                     SPAKIIIFDESTSSLDMITEDRIINKLLSLKNKTIIFIAHRINIAKKTENIVLLDNGKV
                     SDQGTHEELLKRSKLYYNLCKK"
     gene            7305..7457
                     /locus_tag="RZ55_RS07245"
     CDS             7305..7457
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RZ55_RS07245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_171823240.1"
                     /transl_table=11
                     /translation="MRKFDELTEDDLISVKGGFNKTAYKWGRYTRKGVDTIMEVSQVLK
                     FAKYF"
     gene            7513..7671
                     /locus_tag="RZ55_RS07250"
     CDS             7513..7671
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RZ55_RS07250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_171823241.1"
                     /transl_table=11
                     /translation="MGNIIELSESDLSTIYGGFKSYTRRQVYRNCRAEGNGGETAIDFL
                     RGFMDEK"
     gene            7722..8519
                     /locus_tag="RZ55_RS00265"
                     /old_locus_tag="RZ55_05190"
     CDS             7722..8519
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="RZ55_RS00265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RZ55_05190"
                     /product="MFS transporter"
                     /protein_id="WP_057175423.1"
                     /transl_table=11
                     /translation="MFALLSIISVIILVVGIFFTENINEIKKTKLDFVSLLLSVVGFGG
                     LLYAFSEIGNTGNLNLTISIIGLIGIAAIAGFVYRQNHLDEPMINMSIFKNHNFNLTTL
                     LSTLSNIAMVGIELVMPLYLQNSRMVSALVAGLVMMPGTILMGIFNPISGGLYDKLGVR
                     KVSIIDFAFILIGSIPMVVFNASSSLAIIIVSYALRMVGVALVMMNTFTDGINSLPTQY
                     ETDGNAASSNIRQVGGSLGTAISMTVVTLGVNLSSSHASTAGA"
     gene            complement(8648..9445)
                     /locus_tag="RZ55_RS00270"
                     /old_locus_tag="RZ55_05200"
     CDS             complement(8648..9445)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017927.4"
                     /locus_tag="RZ55_RS00270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RZ55_05200"
                     /product="DUF975 family protein"
                     /protein_id="WP_057175424.1"
                     /transl_table=11
                     /translation="MYNRPEQKQEVKGLIRGHQRFFFILFLPFLLTYIAYNVLANQVSF
                     NSALNVDATNVTTNITSNDAVNYVATFIFLILSSIFLLGAIYTAFNVLRGQDDFQNPIR
                     KSLTFIDDQRLFWGTTWTWILRSILLSLWSLLYIPGFLFMLLLPNALGLSLSIAEFIAV
                     TVWVVIKSFAYTQALWVYRDARLANQPVSAYTAIMTSQDMMKGYKVDYLVLQLSFIGWM
                     ILVAITSGIVGIYVFPYYYITQVKFYEFVKEQFKQKQTVLDVE"
     gene            complement(9538..10878)
                     /locus_tag="RZ55_RS00275"
                     /old_locus_tag="RZ55_05210"
     CDS             complement(9538..10878)
                     /EC_number="1.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1175: pyridine nucleotide-disulfide oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010015744.1"
                     /locus_tag="RZ55_RS00275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RZ55_05210"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_057175425.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1e-51, bitscore:
                     169.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNPNFDYDVIYIGSGHAAFDGAKTLCKQGFKIAFVEAIAVGGTC
                     PNWGCNAKISLDGPVTLAKTQRRMKDIVEGDTKLNWKENIKHKREGILPIKVDREATMK
                     SLGMDVIHGFAKFTDAHTLEINGEHVTSDKIVIATGLTPHKVEIEGTELSHDSRDFMEL
                     EDMPKRMAIVGTGYIAMEFATMANEAGADVTMFMHGDKALRPFYQPYVKILMDEMKQSG
                     IKFVENSGLRSFSQDGDEYTVTYADNQQLTVDYILDASGRVPMVHNMGLEEVGVKYDDK
                     KGVLVNEYLQTSVENIYASGDVAATGQPNLTPTATFESQYLKHLFAGETDQPIDFPAIP
                     SVVFTSPRISQMGVTVDEANASDEYTVEEHDATNTWFRQITKEPVVKNAYIFDKEHRLV
                     GFTEISDQADNAVDMIVPAVNMKMTPLEIERNVHLFPSIGHDTWKNI"
ORIGIN
        1 gtaaattcca tatcttctag tagggcgtta tcccacccaa cgttttttgc catgtcgaga
       61 ctcacaaatt gtccattacc agatgcgatt ccatcaccta aattatcacg agtgatttgt
      121 gctttacggt tcattccgca aaactcgaca tcttgagcaa cttctagagc gttattaata
      181 ttaatcattt tcacggctga ttgaaccata ccaataccag gattttcttg gaagtcaaaa
      241 atagcggttt cgatatcttc agaactcatt cgaccatccg catcgataat taagataata
      301 ttctcagaat actcgttttt gtgttgatca atataagatg ttgcgaaatt taacactgac
      361 cctttacccg tatgggcaat gtcagaagta gtttgcacca cgatttcttt cttcgattga
      421 gcaatcgcaa aagtatcgtc actggattga tcatcgacga ctaaaatatg accggtaata
      481 tttgaatcaa gcttattaat ctcgtttctt aatgaaacaa tggtatcacc gatgacattt
      541 tcttcgttca atgctggaat tacaatccac aaacagtatt gtgagacaat attaattttt
      601 cttttcttgt gtttataatg ttttgaatga ttaaccaata gaaatagtac ttgtaatata
      661 tatagaatat aaaaaatcat cgatattaca aatagaattt tcactttatc tacctccgtt
      721 ttcgggacaa gataacttca ttatattcag aagtttataa atgtcttgtc tatccgaaat
      781 gcataggtag tatatgatga cacgatgtct ctactgggat tgagcgattt taagttttta
      841 gactatatgt catgttcacg tgattaatat ttcttttata taaacttatc taaaaaaagg
      901 aaaattttac ccattttttg acaaactatc caattattca gcttccacac tgatgatttt
      961 ttgttgatcg aaatcgtata acccttggtc ggttagtttc aatgttggaa ttactggtag
     1021 tgttaagaat gatagggtga tgaatgggtc gaaatcgaca ccatcactaa tttctttaaa
     1081 cgctaactcg atttcttcta gttcgtgact ggctgcttgc catgatttat cagacattaa
     1141 tccagcaact ggtagtggca tggtggctaa agtcttttct tcgtcaacta cggcaatgcc
     1201 acccttagta gcggtaactt cttggattgc gcggtagatt gcttccttgg tggttccgac
     1261 agcaacgatg ttatgggaat catgggcgac agtagtggca atagcaccgt ttttaatttt
     1321 aaaaccatga actagtgcca atccataagt acctagttgg tggtgacgtt ctgcgtcgac
     1381 catcaataat atgtcgtttt ctaaatcggt tttgaaataa ccatcttcta taggaacttc
     1441 ttggatggag tgggtagttt caatgtgatt tgcaattaca cccattacat ggcatttatt
     1501 tgagtttaaa ggtagtttta aatcatcaac tgataggttt tggtggacgc agttgtcgcc
     1561 gaatttaatt ggggtggtat taaaactatc ttcggtcatg accttaccat ttttgatgat
     1621 ggtcttaacg tcgacttggt ctaggtgatc aacgattact aaatctgctt ggtagtcttc
     1681 cttgatggca cctaacttat ctagtttgtg ggcaacggca gcgttgtagc tagcaatctt
     1741 atatgcagtt tctggtttta gaccatattg aattgataac ttaatattga agttaatgct
     1801 accttctcga atgatggtag tgatgaactt atcatcggta cagaatgaaa aacggtctga
     1861 attatcttct gtaactgctt gaatggtgtt ttgtaaatct ctttcaacgg ttccttctct
     1921 taagaagaca ttcatatcgg cagcaagtct gtcgttcatt tggtctaggc tgaccgattc
     1981 gtggtcggta gtaatgccat tttcacgata ttccttaagt tgttccaagg ataatccggc
     2041 gccgtgacca tcaatttggt atccgtggtc taaggcatca tttagttttg ccatcatgtc
     2101 tttatcctta ttatgtacgg ctgggtagtc cattacttcg gctaaaccga ttacggactc
     2161 ttcattatat agtaggtgca agtctttcgc ggtcaaagta gcaccgttat gttcgaaact
     2221 ggtagcggga acggatgaag gtaacatcac taatacttcc agtggagttt gtttggcatc
     2281 ttcaatcatg tatttaatcc cgtcggaacc tgaaacgttt gcgatttcgt gtggatcgac
     2341 gacgatagaa gtaacaccca tgggcactaa aaccttaccc aattcagatg gagtgactag
     2401 tgaactttcg atgtgaacgt gtgaatcaat aaaaccagga acaacaaatt gattcttagc
     2461 gtcgataatt tctttagctt ttaactctgg atgattgccg accgcaataa ttttgccatt
     2521 attaatgaaa atatcattaa tttcgaatct gtttgtgaat acatttaaaa cttttccatt
     2581 ttgaacaagt aaatcgattg tttgcatttt cccaccccaa cgttaattta attttgatta
     2641 aaattatcaa gtattatgaa acattaatga aagaaagtca aatctatttt aatgttcggt
     2701 aaatctgcgt agtattaagc ctttaataga tatattttct atacttgata gagtaatttt
     2761 tcagtagtac ctaaaaaaat gtttaaaaag ggggaaatgt agtgtacaat tgtattgttc
     2821 ttaaaaatag aaagggtgaa gaactttgag tgaaaacgtt tcacatgaaa aaaagggctt
     2881 cgttgaatat gccaacggtc cttcactaga agaaattaac ggtggaatta aagttccgaa
     2941 ggactgggga ttctggaaga ctttattcgc ttactccgga ccaggtgcat tagttgcggt
     3001 tggttacatg gatccaggta actgggctac atcaattaca gggggtcaag attttggtta
     3061 cctattgatg tctgtcatct taatttcaag tttgattgcg atgttacttc aatacatggc
     3121 ggctaaactt ggaatcgtga gtcagatgga tttggcacaa gccattcgtt caagaacctc
     3181 aaagacactt ggtgttgttc tatggattat gacagaatta gcaatcatgg ctactgatat
     3241 cgccgaagtt atcggtgggg ccattgcctt atatctttta ttccacattc ctctaatctt
     3301 agcggtattc cttaccgtat ttgatgtttt attattgcta ttattaacta aagtgggatt
     3361 cagaaagatt gaagcaatcg ttgtttgttt aattcttgtt attttagtag tcttcattta
     3421 tgaagttgta ctatcaaaac ctgattggtt aggtgctttc aaaggtttaa ttcctgttga
     3481 aagaacattt tctccaaatg gacctgttaa tggattaact ccattaaagg gtgctttagg
     3541 tattattggt gctactgtta tgccacataa cctataccta cattcagcta ttgctcaaac
     3601 tagagctatt gatcacgacg atgaagaaga cgtagcgaga actgttcgtt ttgctacttg
     3661 ggactcaaac atccaattaa cattcgcatt cttcgttaac gctttactat taatcatggg
     3721 tgtggccgta ttcaagactg gtgccgttca agattcatca ttcttcggtc tattcgatgc
     3781 tttaaacaat actaagatgc tatcaaaccc aattctaatt ggtgttgcta agtctggtat
     3841 tttatcaaca ttatttgccg ttgcattgct tgcttcaggt caaaactcaa ctattactgg
     3901 tactttatca ggacaagtta tcatggaagg tttcgttaac ttaaaggttc ctctatgggc
     3961 tcgtcgttta attactcgtc taatctcagt tatcccagta cttatctgtg tcggtttaac
     4021 tgcaggtgaa tcgaccgttc aacaacatgc tgcaatcaac gatttgattg aaaactcaca
     4081 aattttccta gccttcgcat taccattttc aatgcttcca ttgttaatga tgactgacag
     4141 taaagaagaa atgggtagcc gatttaaaaa tactttctgg gtaaagatcc ttggatggat
     4201 ttcagttctt gtattaattt tcttaaacgg atacaacatg cccgatgctg ttgttggatt
     4261 cattagtgat aatccaaccg ctgctcaaaa tgcacatgct gctgtaattg cttatgcaat
     4321 taatgctgta attctagcat tgttaatctg gacattggtt gaactatacc taggtaacaa
     4381 acgcttaaga aacagagaaa actaaaataa aaaattcaag aatagaaggc gaaaattatg
     4441 caaaacatgg acgtcgaaat taatttcaag aatattttgg ttgctgtcga tgattcagat
     4501 gacgctttat tagctttcaa gtacgcgatt tcacgtgcta aatcagaagg cgctaagcta
     4561 accatcgttt cagtactaga agatgaagaa atcaatgttt tccaatcatt atcaggacaa
     4621 tttgttcatg gtaagtacga agatttagaa aaacatctag aaaaatacaa gaagaccgct
     4681 gaagaagccg gcgtagccga tgttaatgta atcgcatcaa ccggtgaagc tggggaaacc
     4741 atcatcaagg atgtgcttcc taaggttaac cctgatttat tgattgttgg atcagaatca
     4801 aagaagggct tagctcgtca ctttggtagc caagctgcct acatggcaaa atattcacca
     4861 gtatcagtta tggtgattag ataaaactat tgattaatta ctcactccga aaaaaagact
     4921 caagatagta tcttgggtct ttttttgttt gcatataata tttaacgatt atttaacata
     4981 acgttccagt tatcgtcgtt ctatttttta catagattat agtagagttt acttcttttt
     5041 aataattctt catgagttcc ttgatctgaa acctttccat tatctaacag tactatgttt
     5101 tctgtttttt tagcgatatt tattctatga gctataaaaa taattgtttt atttttcaaa
     5161 gatagaagct tatttattat tctatcttct gtaatcatat ctaacgaact agtagattca
     5221 tcgaaaataa ttattttagc aggacttaat aatgctctag ctattgttat tctttgcttt
     5281 tctccaccag acaaaacaac accattttca gagatgtttg ttttaaaccc taaaggtgta
     5341 gactctatta ggtcatttat ttcagatata tcacaggctt cctttatatc attataattt
     5401 aatccgtctc tacaccctaa aagtatatta tctagaaagg taccgtttaa caagtagggc
     5461 gtttgaggca cataattgat atatgatctt aaagtctttc tatttatttc ttttatatta
     5521 tgaccattta tttctatatt tccatattct gcattataaa aaccatttaa aagatgtgct
     5581 attgtagatt tacctgatcc actgattccg acaatcgata ttttttgatt tttatatata
     5641 cttaaattta ctcctgctaa aacctttttg tcataaccat agctatagaa aacattatta
     5701 aaagtaactt taccatctag ttctttaaag ttatctattt ttcttttttc tttgaattca
     5761 gatttaacgg tcattacttc atttagacga ttattagata cttgagcaga ctgtatcttt
     5821 gtttgcaatg aaattatgtt ctctattggg gatataaagt aagatataag agcactgaat
     5881 gccataagct gacctatact taatttattt cttataacta gttcagagcc taaccaaaga
     5941 atagcaacgt tcattattaa tttaatcgat gtctttaaag attcttgcat gaatattaac
     6001 ctactatatt taagagtctt ctctaaataa ttgttaaatt cgtcttttat ttttttatag
     6061 caatcgtttt cagaatttaa agacttaatg gtctctattc cttttaagtc atcaataata
     6121 gatgaactta attttgaatt gctttccatc acttgattgt ttaatttttt gaatggcttg
     6181 aagaaaatat atattataag ggaatatgca gggataagta ataatgtaac caaaaaaagt
     6241 gtataacttt gtattattag gaataatgat aatatcagta tgataaaagt gtctaaaaaa
     6301 aacgaaataa ttgtattagc taaggcatca attatcttat ttgcatcatt aaacctagaa
     6361 acaatctctc cagtttttct actagagaag aatgacattg gtaaattaaa gatgtgatta
     6421 atatatttta atataatatc aatagacaat tttctgttaa gaatgcttac taaatagttt
     6481 tgtataaacg acatagtttg ttgtattacg taagaaaaaa ttaaacataa ggaaaaaata
     6541 ttaattaatt ggaagttttt gttaggtatg agtttatcaa caagtatttc aatataatat
     6601 gatccgatta ttccaattaa agttatacat aatgaggatg aaataatttc agagacaagt
     6661 tttttatact taaagatata tttaaagtag ttccatatac ttttaccagt gtgctttata
     6721 gggatatatt tctcgtttgg atggaataat aatgctattc ctgtccactc atcaacaaat
     6781 gtatttatat tcatttttat tattccaaca ttaggatcag gatctgcaat ctttatataa
     6841 ttattttttt tttcgaaaac aacataaaaa tgataaaacc tattgtcttt taaaacttgt
     6901 acgattattg ggtaattaag atttttttct ttaaatagtg ataaatctgc ttttattgct
     6961 ttagtttcta aattaaaatg ttttgcagct tttatcaatc ctaaagctgt tgttccatta
     7021 actcccgttt tagataaatc tcttaaacta gaaagtgaac agtaagagtt atattttctt
     7081 aaaactgtag ccaatgctgc tacgccacaa tctgattcat ccatctgggg aacataatat
     7141 cttgatatca ttaaaattca ccaaccatta tataaactat attatctata attataatgt
     7201 atatcatttt tttggcaacc ctaaaaaata tatttactta taactaaaag tgggatatat
     7261 tatagatgca aatatttgta atcagaatta tggaggtgaa tattatgaga aaatttgatg
     7321 aattaactga ggatgattta attagtgtta aaggtggatt taacaaaaca gcatataaat
     7381 ggggtagata caccagaaaa ggtgttgata caattatgga agtaagtcaa gttttaaagt
     7441 ttgcaaagta tttttagtaa ttaagactaa aaattaaaaa attagtagaa ataatagttt
     7501 ggaggtaatc tcatgggaaa tattattgaa ttaagtgaat ctgatttgtc tacaatttat
     7561 ggaggattta aatcatatac tcgtagacaa gtttatagaa attgtagagc agaaggtaat
     7621 ggtggagaaa ccgcaattga ttttcttcgt ggattcatgg atgaaaaata gatattagat
     7681 atatttataa aaaagactca atatattatc ttgagtcttt tttgtttgcc ctactaagta
     7741 tcatctcagt gattatccta gtagtcggta tcttcttcac tgaaaacatc aatgaaatca
     7801 agaagacaaa gttagacttt gtttcactat tactatcagt agttgggttt ggtggcttgc
     7861 tatacgcatt ctctgaaatt ggtaacactg gtaacttaaa ccttactatt tcaatcattg
     7921 gattgattgg gattgcggcg attgcaggat tcgtatacag acaaaatcat ttggatgaac
     7981 cgatgattaa catgtctatt tttaaaaacc acaactttaa cctaacaaca ttgctaagta
     8041 ctctaagtaa catcgcaatg gttggcatcg aattagtaat gccattgtac ctacaaaact
     8101 cacgtatggt atcagcgctg gttgctggtt tagtcatgat gccaggtacc atcttgatgg
     8161 gaatctttaa cccaatatct ggtggattgt acgacaaact aggagttaga aaagtttcta
     8221 ttattgattt tgcattcatc ttaatcggat caattccaat ggttgttttt aatgcttcat
     8281 ctagtttagc catcatcatt gtgagctatg cacttagaat ggtcggtgta gcgctagtaa
     8341 tgatgaacac atttacggat ggaattaact cattaccaac ccaatacgaa acagacggta
     8401 acgctgcttc ttcaaacatc agacaagttg gtggatcact tggaacagcc atctcaatga
     8461 ccgttgtgac attaggtgtg aacctatcaa gtagccatgc ttccacagct ggggcttaaa
     8521 ttaccggtta ccaatttggt ttctggttaa tgctagggat tgcgttagtt ggtttcttac
     8581 tatcatttag attacctaag aaataaaccc aaacaaaaaa tccataggat aacctatgga
     8641 ttttttgtta ttcaacatct aaaacggttt gtttttgttt gaattgttct tttacgaatt
     8701 catagaactt tacttgtgtg atgtagtagt aagggaatac gtaaataccg acgattccgg
     8761 aagtgattgc aactagaatc atccaaccga tgaatgatag ttgtaataca agataatcga
     8821 ccttgtagcc cttcatcatg tcttgactgg tcataatggc agtgtaggcg ctaaccggtt
     8881 ggttggctaa tctagcatct ctgtataccc aaagtgcttg ggtgtatgcg aaactcttga
     8941 ttactaccca tacggttacg gcaatgaatt ctgcgataga aagggataaa cctaatgcgt
     9001 ttggtagtaa aagcatgaat agaaaaccag ggatatatag tagtgaccat agtgataata
     9061 ggattgatct taaaatccaa gtccatgtgg taccccagaa aagacgttgg tcgtcgataa
     9121 atgttagtga ttttctaatt ggattttgga aatcatcttg tcctctcaat acgttgaagg
     9181 cggtgtagat agcaccaagt aaaaagattg aagataagat taagaaaata aatgttgcaa
     9241 catagttaac ggcgtcatta cttgtaatgt tagtagtaac atttgtggca tctacattaa
     9301 gagctgagtt aaagcttact tggttagcta aaacattgta agcaatgtaa gttagcaaga
     9361 atggtagaaa taggatgaag aagaagcgtt ggtgaccacg gattaggcct ttaacttctt
     9421 gtttttgttc tggacgattg tacatgataa gcctcctgtt ttatttaatg tcttaattat
     9481 accaaaaacc ccggtcgaag aaatctgacc ggggtttaat ttttgattaa gaatgattta
     9541 gatgtttttc caagtgtcgt gaccgattga agggaatagg tgaacatttc tttcaatttc
     9601 aagtggggtc atcttcatgt taacggctgg aacaatcatg tccactgcgt tatcagcttg
     9661 atctgagatt tcagtaaaac caactaagcg gtgttccttg tcaaaaatgt aggcattctt
     9721 aacaactggt tccttagtga tttgtctaaa ccatgtgtta gtagcatcgt gttcttcaac
     9781 agtgtattcg tcactagcgt ttgcttcatc aacggtaaca cccatttgac tgatacgagg
     9841 tgaagtaaat actactgatg gaattgctgg gaagtcgatt ggttgatctg tttcaccggc
     9901 aaataagtgc tttaggtatt gtgattcgaa tgttgctgtt ggagttaggt ttggttgacc
     9961 ggtagcagca acgtcacctg aagcgtagat gttttcaact gatgtttgta ggtattcgtt
    10021 aactaagaca cccttcttat catcgtattt aacaccaact tcctctagac ccatgttgtg
    10081 aaccattgga acacgaccag aagcgtctag gatgtaatca acagttagtt gttggttgtc
    10141 agcgtaggta acagtgtatt cgtcaccgtc ttggctgaat gaacgtaagc cagagttttc
    10201 aacaaacttg atacctgatt gcttcatttc atccattagg atttttacgt atggttgata
    10261 gaaaggacgt aatgccttgt ccccgtgcat gaacatggta acatcagcac ctgcttcgtt
    10321 tgccatagta gcaaattcca tggcgatgta accagtacca acaattgcca tacgttttgg
    10381 catgtcttct aattccatga agtctcttga atcatgtgat aattcagtac cttcaatttc
    10441 aactttgtgt ggtgttaaac cagtggcaat aacaatcttg tctgaagtta cgtgttcacc
    10501 attgatttct agtgtgtgag catcagtgaa cttagcgaaa ccatggataa catccattcc
    10561 taagctcttc atagttgctt cacggtctac cttgattggt agaattcctt cacgtttgtg
    10621 tttgatgttt tctttccagt ttagcttagt gtcaccttca acgatgtctt tcatacgtct
    10681 ttgggtctta gctagagtaa ctggtccatc aagtgagatt ttggcgttac aaccccagtt
    10741 agggcaagta ccaccaacgg cgattgcttc tacaaatgca attttaaaac cttgtttgca
    10801 aagtgtcttg gcaccgtcga atgcggcgtg tcctgatccg atgtagataa catcgtaatc
    10861 gaagttagga tttttcatgt taaagcctcc gtatttatta cgatttttat attttcttat
    10921 attaattaat gtacaatatt gtagtgttgc aaacaaatat attgactcga ttttaatgga
    10981 attttaaaca ataaaaaaac cacccaaatg ggtggtttta cttaatattt tatagatgtt
    11041 gccaagcgtc actaccgata gtagggaaca agtgaacaat cttgttaatt tgagctttgt
    11101 ctaattttag agcgacggca ggtaggattg aatcaattac gctatccgct tcgtttgata
    11161 cttcagtggc accaactaag tggtgttcct tgtcgaagat gtatgcacgt ttggcaattt
    11221 cttcgttagt gattgaacgg taccaatcag cagtcatatc gtgttcttca acagtgtagt
    11281 cgtcactgtt cttagcttct tcaactgata caccgacttg ggcgatacga ggtgaagtga
    11341 acactactga agggatggca gggaaattga tggcatcctt agtttcgcct gagaataggc
    11401 tagttaggta ttgtgattcg aagattgcag ttggagtcaa tcttggttga ccagtagcag
    11461 caacgtcacc tgaagcataa atgctatcaa ctgatgtttg caagtgatcg tttacttcaa
    11521 taccggtctt cttgtcgtat ttaacaccga cttcgtctaa accaatgttt tcgacgtttg
    11581 gaatacgacc agtcgcatct aagatgtagt cagtgaagac ttgacggtta ttagataatt
    11641 caatggtagt cttgcacttt tcttcgtgga attcgtttac ttcggcgttt tcaacgaagg
    11701 taacaccttt ttcctttagg tcatttacga cagcgtcaac gtattcaccg ttgaagtcac
    11761 gaagtgcgcg gtcaccatgc atgatgacag taacttgtga tccagcagcg ttggcgattg
    11821 atgcaaattc catggcgatg taaccggcac cgatgatggc gatgtgaact ggtagttcat
    11881 tgatatttaa gaagtccttt gaatcgtggg ccatttcttt accaggtaca tctagtctgt
    11941 gtggacgtaa accagtagcg acaacaatct tgtctgcggt gtatgacttg ccgtcaacat
    12001 caacagtgtg gccgtcggtt aacttaccgt agcccttaat aacgtcgata ccaaatgatt
    12061 ctagaccacc ttggatggcg tttggaaggc tatcgatgac cttatgcttg tttagcatgt
    12121 tttgggtcca atcgatgctt aggtttcctt t
//
