LOCUS       NZ_BBZG01000005        10870 bp    DNA     linear   CON 12-AUG-2024
DEFINITION  Nonomuraea pusilla strain NBRC 110462, whole genome shotgun
            sequence.
ACCESSION   NZ_BBZG01000005
VERSION     NZ_BBZG01000005.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nonomuraea pusilla
  ORGANISM  Nonomuraea pusilla
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft Genome Sequence of Nonomuraea sp. TP-A0861, a Producer of
            Myxochelin A
  JOURNAL   Genome Announc 3 (6), e01430-15 (2015)
   PUBMED   26659677
  REMARK    DOI:10.1128/genomeA.01430-15 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650263)
  AUTHORS   Hosoyama,A., Kimura,A., Ichikawa,N. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2015) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, Biological
            Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.8
            Genome Coverage                   :: 107x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417755.1-RS_2024_08_12
            Annotation Date                   :: 08/12/2024 01:46:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,307
            CDSs (total)                      :: 8,242
            Genes (coding)                    :: 8,159
            CDSs (with protein)               :: 8,159
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 21 of 83
            Pseudo Genes (incomplete)         :: 72 of 83
            Pseudo Genes (internal stop)      :: 5 of 83
            Pseudo Genes (multiple problems)  :: 15 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:25:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 440820
            Orig. end                         :: 451690
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBZG01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10870
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10870
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10870
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5870](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5870
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    1189..1202
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.19"
                     /tool="antismash"
     misc_feature    complement(1191..1204)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.19"
                     /tool="antismash"
     gene            1204..1728
                     /locus_tag="ASC57_RS34980"
     CDS             1204..1728
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894902.1"
                     /locus_tag="ASC57_RS34980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_055508221.1"
                     /transl_table=11
                     /translation="MTANDDVTAEVRGWFAGRLPEGWFTGAPEIFRDREEIAVLGTLPD
                     PPADLPEPERAALIEGLVRRFREETRERRIEIAGEAERRFRQKVSWGVVCGGEAVMFTT
                     LSVPVMTRLRQAERQVLDTLVAAGVARSRSDALAWCVRLVGKHTDTWLAELRDALQHVD
                     RIRASGPDVNS"
     misc_feature    1741..1754
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.04"
                     /tool="antismash"
     misc_feature    1755..1770
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.47"
                     /tool="antismash"
     misc_feature    complement(1777..1792)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.37"
                     /tool="antismash"
     misc_feature    complement(1822..1835)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.64"
                     /tool="antismash"
     misc_feature    complement(1823..1836)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.54"
                     /tool="antismash"
     misc_feature    complement(1824..1837)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.51"
                     /tool="antismash"
     misc_feature    1843..1861
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.99"
                     /tool="antismash"
     misc_feature    complement(1901..1916)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.63"
                     /tool="antismash"
     gene            1982..3775
                     /locus_tag="ASC57_RS34985"
     CDS             1982..3775
                     /EC_number="4.1.1.32"
                     /GO_function="GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0017076 - purine nucleotide binding
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133322.1"
                     /locus_tag="ASC57_RS34985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="WP_055508222.1"
                     /transl_table=11
                     /translation="MSVSVEVPAPTTNRELASWVNRIAALTQPDRIEWCDGSEEEWTRL
                     TNLLVEQGTLTRLAARPNSFYAKSDPSDVARVEDRTFICSEREEDAGPTNNWIAPDEMR
                     RTFGELFKGCMRGRTMYVVPFCMGPLGGEISQVGVEITDSPYVAVSMRIMTRMGERALR
                     LIEQRGDFVKAVHSVGAPLEPGQKDVPWPCSSTKYISHFPETREIWSYGSGYGGNALLG
                     KKCYALRIASVMARDEGWMAEHMLILKLTPPAGEARYVAAAFPSACGKTNLAMLQPTIP
                     GWKVETIGDDIAWMRYGDDGRLYAINPEAGFFGVAPGTGEVTNANAVKTLWGNSIFTNV
                     ALTDDGDVWWEGLTEEPPAHLTDWKGRSWTPDSEEPAAHPNARFTTPAAQCPTIAPEWQ
                     DPKGVPISAILFGGRRATAVPLVTESLSWEHGVFLGANVASEKTAAAEGKVGELRYDPF
                     AMLPFCGYNMGDYFAHWLEMGRREGAKPPRIYYVNWFRKGADGRFLWPGFGENSRVLKW
                     IVDRLNGEAKAVPTPIGLLPADLDTEGLDLSEEDLRTLLSVDRDVWREEAARIPAHFET
                     FGDHLPKQLWDEYQALLDRLG"
     gene            4043..4999
                     /locus_tag="ASC57_RS34990"
     CDS             4043..4999
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="ASC57_RS34990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_055508225.1"
                     /transl_table=11
                     /translation="MDSVLLLTSGALAALTILNVTAMVRERAAIRAAAAGARPRGLGAC
                     EVAYLAGGPRRVAVTALAASAMSGTLRMSRGGGVQAVAGAPQAIDPVERELLGRVAPGS
                     VHHAGALRREVARGEAVYAIRTHLTGLGLLVPERRTGTWHRRLAWLRVLSTLALIVGAF
                     QLFMTLGDGGWTRLPGLVLAAATFVGGWVAARVGGSWLPGALTREGLEELETMRRLVPP
                     GSAGAAPALAVALHGLSEIRDADLRHALDAGAVAGSRGRRSSCAAGSCGGGGGSWGCGG
                     GHSGGGHSGGGCGSGASCGGGSGCGSGCGGGCGGGGD"
     misc_feature    4058..4060
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..5870
                     /locus_tag="ASC57_RS34995"
     CDS             5001..5870
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545141.1"
                     /locus_tag="ASC57_RS34995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_055508227.1"
                     /sec_met_domain="DUF692 (E-value: 1.7e-81, bitscore: 268.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGEPAGSRLGVGIGWRPELDLTIERMAGVDFLEVVAENVRPARL
                     PESLRLLRARGLLVVPHGVGLGVGGAEPPGPARLAHLAACAQALGSPLVSEHLAFVRGG
                     GLEAGHLLPVPRTREALDVVTANVRMAQDALPVPLALENVAALFGWPDDELTEAEFLAE
                     LVERTGVLLLVDVANLYANQVNLGLEAVAALDALPLERLAYVHVAGGYERHGVWHDTHT
                     TSVPEAVLDLLTELCARVEPPGVLLEWDDHYPSDAALAEELARVRSAMAGTAADGTGTA
                     GTTLGERR"
     gene            5867..6259
                     /locus_tag="ASC57_RS35000"
     CDS             5867..6259
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC57_RS35000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_055508229.1"
                     /transl_table=11
                     /translation="MTATGSGPPGGPSAVSGDGARQRLARAQARVVAALVAGAAPPEGF
                     DGERLRVQAASLLAKRRSVVARLRPDAAAAAGPRLAEEFAAYAGAREAPPPGYRADADD
                     FAAWLRARGLMPLEPPGGRSWWRRLR"
     misc_feature    6304..6318
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.64"
                     /tool="antismash"
     gene            6404..6877
                     /locus_tag="ASC57_RS43280"
     CDS             6404..6877
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545138.1"
                     /locus_tag="ASC57_RS43280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_236715083.1"
                     /transl_table=11
                     /translation="MTLELRRDGDPYGSVGERCGWLLARLARGVEEARAGTGDARRWPD
                     PDDRFPDEEPDSELFSFRYRARSELVGGGELRCQLRTIPLWVPRRGRRGEWRLTRRAFV
                     EAWSATGVGLRAVLTSAELAAFVRGLVEEAEGCLGGRDLARNPVEGAGRGESR"
     gene            6933..7694
                     /locus_tag="ASC57_RS35010"
     CDS             6933..7694
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545137.1"
                     /locus_tag="ASC57_RS35010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isoprenyl transferase"
                     /protein_id="WP_055508232.1"
                     /transl_table=11
                     /translation="MGLRDLLYRLYEHRLESKLSKDSIPRHVGVIIDGNRRWARAMGMR
                     DVSKGHRKGADKISELLGWCRETGVEVVTVWMLSNDNLNRPSEELEPLLRIIEDVVQEL
                     VAEGWRISPVGALDLLPDRTANVLKTAKEESSDRPGLIVNVAVGYGGRREIADAVRSLL
                     QEHASKGASIEDLVEVLDVEHIAEHLYTRGQPDPDLLIRTSGEQRLSGFMLWQSAHSEF
                     YFHEAYWPDFRRVDFLRALRDYAARHRRYGS"
     gene            7901..9220
                     /locus_tag="ASC57_RS35015"
     CDS             7901..9220
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133320.1"
                     /locus_tag="ASC57_RS35015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PhoH family protein"
                     /protein_id="WP_055508234.1"
                     /transl_table=11
                     /translation="MAVSSSNPSTKPTQRTYVLDTSVLLADPAAMTRFAEHDVVLPIVV
                     ITELEGKRHHPELGYFARKALRYLDDLRIQYGRLDEPMPTGEGTIRVELNHSDPSILPV
                     GFRLGDNDTRILTVARSLAAEGRDVVLVSKDLPMRVKAASIGLAAEEYRAELVVESGWT
                     GMAEMQVTTEDVETLFEHGTAELEEARDLPTHTGLRLLSAKGSALGRVLPDKSVRLVRG
                     DREVFGLHGRSAEQRIALDLLMDPEIGIVSLGGRAGTGKSALALCAGLEAVLERRQHRK
                     VVVFRPLYAVGGQELGYLPGSEGEKMGPWAQAVYDTLGAVTSPEVIDEVLDRGMLEVLP
                     LTHIRGRSLHDAFVIVDEAQSLERGVLLTVLSRVGANSRVVLTHDIAQRDNLRVGRHDG
                     VVAVVEKLKGHPLFAHVTLTRSERSPIAALVTEMLEDITL"
     gene            complement(9222..9740)
                     /locus_tag="ASC57_RS35020"
     CDS             complement(9222..9740)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC57_RS35020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_055508236.1"
                     /transl_table=11
                     /translation="MRPRTLLTAAALCATLAGLSACGISPTDVEDRGAAPTIHVPPPSK
                     TIFLIRNGELTKEPADVGNNTVGSLLGALFAASTEPLTDGRDTALRGFALVRIKDSLNP
                     ITRDEVQLPRTSTLTVYIKGEGTLSKLGKAQIVCTAQQDAAFEEVKIVRENDDRPPTSE
                     GRYICGDLK"
     misc_feature    10135..10149
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     misc_feature    complement(10770..10783)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.12"
                     /tool="antismash"
ORIGIN
        1 ccggcgccgt ccgcgtgcgc acggcgcccc ggcagacgct catggcccag gccaagaccg
       61 cccagccggt cgcgccgctg cgcgggctgc acaagccgaa cctgttcgtc ctcacccgcg
      121 agccgttcac gcaggagctg ttacagaagg tggcgcggct gcgcggagtg cgggcgctcg
      181 aactcgccga cgccgcgtcc gtggtgctcg acggcaaacg cgtccagacc ctcggcgtag
      241 acccttccac cttccgcgcg tacacgccga agacgaccgc ctcctccgac gcgctgtggc
      301 agaacgtcgc cgccggggac gtggccgtgt ccttcgtcct gggcaacgac ggcgggctca
      361 gcctgcacag ccgggtgagc ggcccccggg ggccgctcag gatcggcgcg tacgccacca
      421 ccgggttcgg cgccgtcgac gccgtcgtct ccaaggacac cggacgcgcc ctcgggcttc
      481 cccgcgacaa cgcgctcatc gtcagcgcgc ccaccaccaa ctccgacacg ctgcgcagac
      541 ggctgctgaa ggtgctgccc aagggcaccc aggtggccat ggtcaacccc gtgctgcccg
      601 ccgcgcccac ccgcacccag aactggcccg ccggggcctt catgacgggc gagcagctca
      661 ccgtcgcgct cagggccgcc gtgtccaagc tcggccggcc gtacgtgtgg ggggccgagg
      721 ggcccgacac gttcgactgc tcggggctcg tccagtgggc gtacgggcag gccggggtgc
      781 ggatgccgcg cgtcacccac cagcagtggg tgaccggccc ccaggtgccg ctctcgcagg
      841 cccagccggg cgacctgctc ttctggcgga acgacccgac caaccccgac tacatctccc
      901 acgtggccat ctactggggc gacggcaaga tgctgcacgc cccgagaacc ggggacgtcg
      961 tcaagatcgc gccggtgaac acgcgcaacc tcgcgggcgt cgtccgcgtc agccccgccg
     1021 tggcctcccg cgtccgctga cgggaacccg gcccgaggaa gcgcccagga ccccaggaac
     1081 atccgcgtgg tggggcggcg gcgctcgcag ggccacgcat cgccggcctt ccccggtcat
     1141 cccggagaca caccggccgt gcgccgggct gctttttctg ggcaagcgtg taatgatgat
     1201 tacatgactg ccaacgatga cgtcaccgcc gaggtgcgcg gctggttcgc gggccggttg
     1261 cccgaggggt ggttcaccgg ggcgccggag atcttcaggg accgcgagga gatcgcggtg
     1321 ctcggcacgc tgcccgaccc gcccgccgac ctccccgagc ccgagcgcgc cgcgctgatc
     1381 gaggggctgg tcaggaggtt cagggaggag acgcgggagc ggcgcatcga gatcgccggg
     1441 gaggccgagc gcaggttccg ccagaaggtc tcctggggcg tcgtgtgcgg cggcgaggct
     1501 gtgatgttca ccacactttc ggtgcccgtg atgacccggt tgcgacaggc ggagcggcag
     1561 gtcctcgaca cgctggtggc cgccggagtg gccaggagcc gcagcgacgc gctcgcctgg
     1621 tgcgtccgac tcgtcggcaa gcacaccgac acctggctcg ccgagttgcg cgacgcgctc
     1681 cagcacgtcg accggattcg cgcctccggg cccgacgtca attcctgaga gcagccagcg
     1741 gaaacggtcg ttggaatgga ctaaaccact gtggaaattg gtttagacca tggctttcgg
     1801 agtaaacacc ggactcggtg gctttctttt aaaacctgcg cgcctgggta ggctccccca
     1861 atcgtcgtgg ccgcctgcct gcgacgacgg cgtcaggccg acaggtctac gccaattggc
     1921 ttttacgccg tgtcgtcgtt gatgatgtct tggccgcgga gagagtggag gagtccctgc
     1981 cgtgtccgtt tctgtggaag ttcccgcccc gacgaccaat cgtgaattgg cttcgtgggt
     2041 gaaccggata gccgccctga cccagcccga caggatcgag tggtgcgacg gctccgagga
     2101 ggagtggacg cgtctgacca acctcctcgt cgaacagggc acgctcacgc gcctggccgc
     2161 caggccgaac agcttctacg ccaagtcgga cccgtcggac gtcgccaggg tcgaggaccg
     2221 cacgttcatc tgctccgaac gcgaggagga cgcgggcccg accaacaact ggatcgcgcc
     2281 cgacgagatg cgccggacct tcggcgagct gttcaagggc tgcatgcggg gccgcaccat
     2341 gtacgtggtg ccgttctgca tgggcccgct gggcggcgag atctcgcagg tcggtgtgga
     2401 gatcaccgac tccccgtacg tcgcggtctc catgcggatc atgacccgca tgggtgagcg
     2461 ggcgctgcgc ctgatcgagc agcgcggcga cttcgtcaag gccgtccact cggtgggcgc
     2521 cccgctggag cccggccaga aggacgtgcc gtggccgtgc agctccacga agtacatcag
     2581 ccacttcccc gagacgcgcg agatctggtc gtacggctcg ggctacggcg gcaacgccct
     2641 gctgggcaag aagtgctacg ccctgcgcat cgccagcgtc atggcccgtg acgagggctg
     2701 gatggccgag cacatgctca tcctgaagct cacgccgccg gccggcgagg cgcgctacgt
     2761 cgccgccgcg ttcccgagcg cctgcggcaa gacgaacctc gccatgctcc agccgacgat
     2821 ccccggctgg aaggtggaga cgatcggcga cgacatcgcc tggatgcgct acggcgacga
     2881 cggccgcctc tacgcgatca accccgaggc cggcttcttc ggcgtcgcgc ccggcaccgg
     2941 cgaggtcacg aacgccaacg ccgtgaagac cctctggggc aacagcatct tcacgaacgt
     3001 cgccctcacc gacgacggcg acgtgtggtg ggagggcctc accgaggagc cgcccgcgca
     3061 cctcaccgac tggaagggcc gttcctggac gcccgacagc gaagagcccg ccgcccaccc
     3121 gaacgcgcgt ttcaccacgc ctgccgccca gtgcccgacc atcgcgccgg agtggcagga
     3181 cccgaagggc gtgcccatct cggcgatcct gttcggcggc cgccgcgcga cggccgtgcc
     3241 gctggtcacg gagtcgctga gctgggagca cggcgtcttc ctcggagcca acgtcgcctc
     3301 cgagaagacc gccgccgccg agggcaaggt cggcgagctg cggtacgacc cgttcgcgat
     3361 gctgccgttc tgcggctaca acatgggcga ctacttcgcc cactggctgg agatgggccg
     3421 ccgcgagggc gcgaagccgc cgcgcatcta ctacgtcaac tggttccgca agggcgcgga
     3481 cggccggttc ctctggccgg ggttcggcga gaacagccgc gtgctgaagt ggatcgtgga
     3541 ccggctcaac ggcgaggcca aggcggtgcc cacgccgatc ggcctgctgc cggccgacct
     3601 cgacacggag ggcctcgacc tgtcggagga ggacctgcgc acgctgctgt ccgtcgaccg
     3661 cgacgtgtgg cgcgaggagg cggcgcgcat cccggcccac ttcgagacct tcggcgacca
     3721 cctgccgaag cagctctggg acgagtacca ggcgctgctc gaccggctgg gctgacccgc
     3781 cgcccgcgtg gcgccgcctg gcgcgcccgt ccccgtggcg ggtggcgccc ggcgccccgc
     3841 gagcggtccc gtcccggggg aggcttgagc ggagcctcgc acacggcccc ggcggccacc
     3901 cggccgccgg ggccgtcctc gtgccgggcg gcgcccccgg cgggcccgcc ggtcgatcct
     3961 gccgcgaact ggcgcaactt ccgccccagg cctagcgtct tctcggtacg gacctctgcg
     4021 gccgtaccac ggggaggcgt acatggactc ggtcctctta ctgacgtcgg gggcgctggc
     4081 ggccctgacg atcctgaacg tcacggcgat ggtgagggaa cgcgccgcga tccgggcggc
     4141 ggcggccggc gcgcgtcccc gggggctggg cgcctgtgag gtggcgtacc tcgcgggagg
     4201 gccgcgcagg gtggcggtca cggcgctcgc ggcgtcggcg atgtcgggga cgctgcggat
     4261 gtcgcgcggg ggcggcgtgc aggccgtcgc gggcgcgccg caggcgatcg acccggtcga
     4321 gcgggagctg ctgggccgtg tcgcgcccgg ttcggtccac cacgcgggcg ccctgcgcag
     4381 ggaggtcgcg cggggggagg cggtgtacgc gatcaggacg cacctgacgg ggctcgggct
     4441 gctcgtcccc gagcgccgta ccgggacgtg gcaccgcagg ctggcgtggc tgcgcgtgct
     4501 gtcgacgctg gcgctgatcg tgggcgcgtt ccagctgttc atgacgctgg gcgacggcgg
     4561 gtggacgcgg ctgcccggac tcgtcctggc ggcggcgacg ttcgtggggg gctgggtcgc
     4621 ggcgcgtgtg ggcggctcct ggctgcccgg ggcgctcacc cgcgagggct tggaggagct
     4681 ggagacgatg cgcaggctgg tcccgcccgg ctcggcgggc gccgcgcccg cgctggcggt
     4741 cgccctgcac gggctgagcg agatccgcga cgcggacctg cggcacgcgc tcgacgccgg
     4801 ggccgtggcg ggatcgcggg gacgccggtc gagctgcgcc gcgggcagct gcggcggggg
     4861 cggcgggagc tggggctgcg gcggcggcca ttccggcgga ggccactcgg gtggcggctg
     4921 cggcagcggg gcgagctgcg gcggcggctc gggctgcggg agcggctgcg gcggcggctg
     4981 cggcggcgga ggggactgac atggcggggg agcccgcggg gagcaggctc ggtgtcggca
     5041 tcgggtggcg gcccgagctg gacctcacga tcgagcggat ggcgggcgtc gacttcctgg
     5101 aggtggtcgc cgagaacgtc aggccggcgc gcctgcccga gtcgctgcgg ctgctgcggg
     5161 ccaggggcct gctggtggtg ccgcacggcg tcggcctggg cgtcggcggc gcggaaccgc
     5221 ccggcccggc ccggctcgcc cacctggcgg cctgcgcgca ggcgctgggc tcgccgctgg
     5281 tgagcgagca cctcgcgttc gtgcggggcg gcgggctgga ggcgggccac ctgctgccgg
     5341 tgccgcgcac ccgcgaggcg ctggacgtcg tcacggcgaa cgtgcgcatg gcccaggacg
     5401 cgctgccggt gccgctggcg ctggagaacg tggccgccct gttcggctgg cccgacgacg
     5461 agctgaccga ggccgagttc ctggcggagc tggtggagcg tacgggggtg ctgctgctcg
     5521 tggacgtggc gaacctctac gccaaccagg tcaacctggg gctggaggcg gtggccgcgc
     5581 tggacgcgct gccgttggag cggctggcgt acgtgcacgt cgcgggcggg tacgagcggc
     5641 acggggtgtg gcacgacacg cacacgacgt ccgtgccgga ggcggtgctg gacctgctga
     5701 cggagctgtg cgcgcgggtg gagccgccgg gcgtgctcct ggagtgggac gaccactacc
     5761 cgagcgacgc cgcgctggcg gaggagctgg ccagggtcag gtccgccatg gccggcaccg
     5821 cggccgacgg cacgggcacc gcgggcacca cgctggggga gaggcgatga ccgccaccgg
     5881 gagcgggccg cccggcggcc cttcggccgt gtccggcgac ggggcgcggc agcggttggc
     5941 gcgggcgcag gcgcgggtgg tggcggcgct cgtcgcgggc gccgcgccgc cggaggggtt
     6001 cgacggggag cggctgcggg tgcaggcggc gagcctgctc gccaagcggc gttcggtggt
     6061 ggcgaggctg cgcccggacg cggccgccgc ggcgggcccg cgcctggctg aggagttcgc
     6121 cgcgtacgcc ggggcgcgcg aggcccctcc gcccggctac cgggccgacg ccgacgactt
     6181 cgccgcctgg ctgcgcgccc gcgggctcat gccgctggag ccgccgggtg ggcggagctg
     6241 gtggcgccgc ctccgctgat cccgacaaaa gggacatttc tccacgttta gcgaacttta
     6301 aggtggaaac cgtgtctata tggggggact acgctgtgtg gacggatctt acctaaccgt
     6361 cacacccgtc gcatgtacgg acagcgtcgg cgcaccgtac gaggtgaccc tggagctgcg
     6421 ccgggacggc gacccgtacg gcagcgtggg cgagcggtgc ggctggctgc tcgcgcggct
     6481 ggcgcgcggc gtcgaggagg cccgcgcggg cacgggcgac gccaggcgct ggcccgatcc
     6541 cgacgaccgg tttcccgacg aggagcccga cagcgagctg ttctccttcc gctaccgtgc
     6601 caggtcggag ctggtgggcg gcggcgagct gcgctgccag ctcaggacga tcccgctctg
     6661 ggtcccgagg cggggcaggc gcggcgagtg gcggctgacc cgcagggcgt tcgtggaggc
     6721 gtggagcgcc accggggtgg gcctgcgggc ggtgctgacg tcggcggaac tggcggcgtt
     6781 cgtccgcggc ctggtcgagg aggcggaggg ctgcctgggc ggccgtgatc tcgcgaggaa
     6841 tcccgttgag ggggcaggca ggggtgaatc gcggtgataa ttgcctgtta ggtcattcga
     6901 ccatgggcac agcaccgctg gggaggaact ccgtgggcct gcgcgatctg ctctaccggc
     6961 tgtacgagca ccggctggag agcaagctgt cgaaggacag catcccccgg cacgtcgggg
     7021 tcatcatcga cggcaaccgg cgctgggcgc gcgccatggg catgcgggac gtgtcgaagg
     7081 gccaccgcaa gggcgcggac aagatctcgg agctgctcgg ctggtgccgt gagaccggcg
     7141 tcgaggtggt caccgtctgg atgctgtcga acgacaacct gaaccggccg agcgaggagc
     7201 tggaaccgct gctgcgcatc atcgaggacg tggtgcagga gctggtggcg gagggctggc
     7261 ggatcagccc ggtcggggcg ctcgacctgc tgccggaccg tacggccaat gtcctgaaaa
     7321 cggcaaaaga agagtcatcg gaccgtccgg gcctgattgt gaacgttgca gttgggtatg
     7381 gaggaagacg tgagattgcc gatgcggtgc gctcactcct ccaggagcac gcgagcaagg
     7441 gggcgagcat cgaggacctc gtcgaggtac tcgatgtgga gcacatcgcg gagcatctct
     7501 acacccgcgg ccaacccgac ccggacctcc tcatccgtac gtcgggcgag cagcgcctgt
     7561 ccggtttcat gctctggcag agcgctcatt ccgagttcta cttccacgag gcctactggc
     7621 cggacttccg cagggtcgac ttcctgcggg cgctgcgcga ctacgctgcg agacaccgac
     7681 gttacggttc ataggccgac aatcgggcat ctaggacgta acgtggtaag tgtccggccg
     7741 caggccggac actccggaga gggcccgcct ttgcgccaac acttgccgag ggggccggag
     7801 tccggcgccg ccggtcgtgt ctggccgggg acgcgggtcc ggtccgattc ccgcctcgtc
     7861 tgcagggtgc ccgcacctat gggcgcccgg gggagaacgt gtggctgtgt cctcgagcaa
     7921 cccttcgacc aagccgaccc agcgtacgta cgtgctggac accagcgtcc tgctggccga
     7981 tcccgcggcc atgacccgct tcgcggagca cgacgtggtc cttccgatcg tggtcatcac
     8041 ggagctggaa gggaagcggc accacccaga gcttggctac ttcgcgagga aggccctgcg
     8101 ctacctcgac gacctgcgga tccagtacgg caggctggac gagccgatgc ccacgggcga
     8161 gggcacgatc agggtcgagc tcaaccacag cgacccctcg atcctgccgg tcgggttccg
     8221 tctcggtgac aacgacacgc gcatcctgac cgtggcccgc tcgctggccg ccgagggccg
     8281 cgacgtcgtc ctcgtctcga aggacctccc gatgcgggtc aaggccgcct cgatcggcct
     8341 ggccgccgag gagtaccgcg ccgagctcgt ggtggagtcg ggctggaccg gcatggccga
     8401 gatgcaggtc acgaccgagg acgtcgagac gctgttcgag cacggcacgg ccgagctgga
     8461 ggaggccagg gacctgccca cgcacacggg cctgcggctg ctgtcggcga agggctcggc
     8521 cctcggccgg gtgctccccg acaagtcggt gcggctggtg cgcggcgacc gcgaggtgtt
     8581 cgggctgcac ggccgctccg ccgagcagcg catcgcgctc gacctcctca tggaccccga
     8641 gatcggcatc gtgtcgctgg gcggcagagc cggcaccggc aagtccgcgc tggccctctg
     8701 cgcgggcctg gaggccgtgc tggagcggcg ccagcaccgc aaggtggtcg tcttccgccc
     8761 cctgtacgcc gtgggcggcc aggagctcgg ctacctgccg ggcagcgagg gcgagaagat
     8821 gggcccctgg gcgcaggcgg tctacgacac gctgggcgcg gtcacctcgc cggaggtcat
     8881 cgacgaggtg ctcgaccgcg gcatgctgga ggtgctgccg ctcacccaca tccgcggccg
     8941 ttcgctgcac gacgcgttcg tgatcgtgga cgaggcgcag tcgctggagc gcggggtgct
     9001 gctgaccgtg ctgagccggg tgggcgccaa ctcccgcgtg gtgctcacgc acgacatcgc
     9061 ccagcgcgac aacctgcggg tcggcaggca cgacggcgtg gtcgcggtgg tggagaagct
     9121 gaagggccac ccgctgttcg cgcacgtcac gctcaccagg tcggagcgtt cgccgatcgc
     9181 cgcgctggtg acggagatgc tggaggacat caccctctag ctcacttgag gtcgccgcag
     9241 atgtaccgcc cctccgacgt gggggggcgg tcgtcgttct cccggacgat cttgacttcc
     9301 tcgaaggcgg cgtcctgctg ggcggtgcag acgatctgcg ccttgccgag cttgctcagc
     9361 gtgccctcgc ccttgatgta gaccgtcagg gtggaggtgc gggggagctg gacctcgtcg
     9421 cgcgtgatcg ggttgagcga gtccttgatc cgcaccaggg cgaacccgcg gagcgcggtg
     9481 tcgcgcccgt cggtcagcgg ctcggtcgag gcggcgaaca gggcgccgag caggctgccg
     9541 accgtgttgt ttccgacgtc ggcgggttcc ttcgtcagct cgccgttcct gatgaggaag
     9601 atcgtcttgg acggcggcgg aacgtggatg gtgggggcgg cgcccctgtc ctccacgtcg
     9661 gtgggcgaga tgccgcaggc cgacagcccg gcgagcgtgg cgcagagcgc ggcggcggtg
     9721 aggagcgtgc gcggtctcat gtgtgcggca gccagaccgt gaacagggtg ccggggttct
     9781 gcctgcgcac cgagaccgtg ccgccgtgga gctggacgtt cgccctggcg atggacagcc
     9841 cgagcccgct gccctggctg cgggcccggc cggcgcccgc cttgaagaac cggtcgaaga
     9901 cgtgggccag cgcctcctgc gggatgccct ggccgtggtc gcgcaccttc acctccagcc
     9961 cgttgacggt ggccctggcg ttgacgagca ccggcggccc gccgtgcttc agcgcgttgc
    10021 cgacgaggtt ggccacgatg acgtcgaacc tcctcgggtc caggttggcg accaggctct
    10081 cgggggcgtt gacccgcacc tggtcgctcc agccgcgcac ctccaggcag tcctggacgg
    10141 cgtcggccac gttcaccggc tccaggttga gcgtcgcggt gccggcgtcg aagcggctga
    10201 tctcgatcag gtgctcgacc agctcgcgca gccgctcgat ctcgtgcagc accagccgca
    10261 ccgcctcggc gggctcctcc ggcagcctgg acgcctcgtc gtggagcatg tcggcgacgg
    10321 ccgtcatcgc ggtgagcggg gtgcgcagct cgtgcgagac gtccgcgacg aacctgcgcg
    10381 acatggcttc gagcgagcgc agctccgaca cgctgagttc gagcgccgcc gccgcgtcgt
    10441 tgaacctgga ggtgagctcg gccagctcgt cctgaccgtg cacgggcagg cgggtgtcca
    10501 gtctgccctg gccgagcgcc tgggcggcgg cgctgaggcg tctgaccggc agcagcacct
    10561 gccgggccga cagcagcgcg acgatcagcg cgatcagcac gatggccacg ccggcctggg
    10621 cgagcgtgct cctcagcgtc gcgagctgct gctcgtcgtc gcgcatcggg aagaacacga
    10681 acgtccgcag cccggtggcc tggggcgcgc gttcggtgtc gcggtagacc tcggcgccga
    10741 cgatgagcca cagctcgttc ttgaccacct tgcgctgcgt gacgaggctc tgcttcgccc
    10801 tgccgtacag ctcgttgggg acgtcgctca tcgtcatggg cccgtcggcg gcgatgaggg
    10861 tgccgtattc
//
