LOCUS       NZ_BBZG01000003        21002 bp    DNA     linear   CON 12-AUG-2024
DEFINITION  Nonomuraea pusilla strain NBRC 110462, whole genome shotgun
            sequence.
ACCESSION   NZ_BBZG01000003
VERSION     NZ_BBZG01000003.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nonomuraea pusilla
  ORGANISM  Nonomuraea pusilla
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft Genome Sequence of Nonomuraea sp. TP-A0861, a Producer of
            Myxochelin A
  JOURNAL   Genome Announc 3 (6), e01430-15 (2015)
   PUBMED   26659677
  REMARK    DOI:10.1128/genomeA.01430-15 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040125)
  AUTHORS   Hosoyama,A., Kimura,A., Ichikawa,N. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2015) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, Biological
            Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.8
            Genome Coverage                   :: 107x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417755.1-RS_2024_08_12
            Annotation Date                   :: 08/12/2024 01:46:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,307
            CDSs (total)                      :: 8,242
            Genes (coding)                    :: 8,159
            CDSs (with protein)               :: 8,159
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 21 of 83
            Pseudo Genes (incomplete)         :: 72 of 83
            Pseudo Genes (internal stop)      :: 5 of 83
            Pseudo Genes (multiple problems)  :: 15 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:25:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 728944
            Orig. end                         :: 749946
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBZG01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21002
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="4"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21002
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21002
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11002](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11002)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    1197..1211
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.85"
                     /tool="antismash"
     gene            1233..2105
                     /locus_tag="ASC57_RS27100"
     CDS             1233..2105
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632716.1"
                     /locus_tag="ASC57_RS27100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_055506028.1"
                     /transl_table=11
                     /translation="MTAAIGHAARQAPGYAPGRTLPLRVEFVRQFKRRRTLVMFGLLLA
                     LPWILVIAFQFGPQSSGQGQSALRISDLATAGGLNFAAFALSVSASFLLVVAVALFCGD
                     TVSSEANWSSLRYLLAAPVPRDRLLRQKLIVALGYSAMAVICLPLMALLAGTLAFGWND
                     VQVPGTGEIIPALEVLPRFGIVIGYALVSQLVVAALAFLVSTMTDSPLGAVGGAVGLWI
                     VCNILQAVEALGSLREFLPTFWNNAWLDALAPEPDMSGMAKGASLSITYAALLIAFAFR
                     RFRHKDVVS"
     gene            complement(2174..2455)
                     /locus_tag="ASC57_RS27105"
     CDS             complement(2174..2455)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC57_RS27105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_055506029.1"
                     /transl_table=11
                     /translation="MARRKDREHFHDRTSDPLQHAAFAVIHRSGWLLGALFGLVQQTAD
                     VANGVPAGCEAWRQGWWEQLAGANGKADIVANAKINDSGLSRSTTRAC"
     gene            complement(3000..3125)
                     /locus_tag="ASC57_RS46080"
     CDS             complement(3000..3125)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC57_RS46080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_269085620.1"
                     /transl_table=11
                     /translation="MHTPTCTVTRTVTCTVTRTTTRTVTGAVTGIATGTAVRFVS"
     gene            complement(3258..4046)
                     /locus_tag="ASC57_RS27110"
     CDS             complement(3258..4046)
                     /NRPS_PKS="Domain: oMT (27-129). E-value: 3.1e-11. Score:
                     33.9. Matches aSDomain: nrpspksdomains_ASC57_RS27110_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546607.1"
                     /locus_tag="ASC57_RS27110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="WP_055506357.1"
                     /transl_table=11
                     /translation="MLDLDAEVLAEHLAFITAWLPLKAEPRHIVDLGCGTGAGTFALLD
                     RFPEAHVTAVDTSTGYLQRLREKACARGVEERVRTVQADLDAADWPDLGTPDLVWASAS
                     MHHMAHPDRTLAGVHDLLAPGGLFAVVELDGFPRFLPADAPENRPGLEERCHRATDGFH
                     AEHVRHRGADWGRMLAAAGFTVEDVRTITVNIEGDGSEAIGRYAQGSLQRVRGAAAPAL
                     SPEDLAALDELLDTESPHSILRRDDLAVRTERTVWAARRA"
     aSDomain        complement(3660..3965)
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC57_RS27110_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="3.10E-11"
                     /label="ASC57_RS27110_oMT.1"
                     /locus_tag="ASC57_RS27110"
                     /protein_end="129"
                     /protein_start="27"
                     /score="33.9"
                     /tool="antismash"
                     /translation="HIVDLGCGTGAGTFALLDRFPEAHVTAVDTSTGYLQRLREKACAR
                     GVEERVRTVQADLDAADWPDLGTPDLVWASASMHHMAHPDRTLAGVHDLLAPGGLFA"
     gene            4011..4472
                     /locus_tag="ASC57_RS44130"
     CDS             4011..4472
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC57_RS44130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_176573456.1"
                     /transl_table=11
                     /translation="MLGEHLGIQVEDLRLAAIIVRRVVATVAVTMATVTVTMAVATVTV
                     TMAVAAITVTKAVAAITVTKAVATVTVTKAVAAITVTKAVATVTVTKAVATVTVTKAVA
                     TVTVTKAVATVTVTKAVAVFGMTVVVAAFAVRGVLVCLGHGDTVRRSCA"
     gene            complement(4561..5166)
                     /locus_tag="ASC57_RS27115"
     CDS             complement(4561..5166)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019607445.1"
                     /locus_tag="ASC57_RS27115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_176573476.1"
                     /transl_table=11
                     /translation="MTQEDGQLESLVRKRIRALRVAQGWSLEELATRANLSQSSLSRIE
                     NGRRRLALDQLVTLARALDTSLDQLVENAADDVVISPMIDGAHGLMRWPIKGDPGMSVM
                     RQRMTDPPPDNPARMRAHPGREWLVVLSGTAILMLGHRRFRIETNQAAEFPTMMPHAIG
                     AEGGPCEILGIFDRDARRGHQRDVAEAGDRGDAQTPYE"
     gene            5482..7071
                     /locus_tag="ASC57_RS27120"
     CDS             5482..7071
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="ASC57_RS27120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_055506031.1"
                     /transl_table=11
                     /translation="MSTNQPPQAAPASGGGTLDERELRAILIAVSVALMAVIASVSGLN
                     VAQTHLAVEFGAPQNAVLWIINVYTLALAALLLPLGAIGDRLGRKPMLITGLTVFGAAG
                     IVAGLAPSTEVMIAARLAGGVSAAMIMPITLAVITSTFPEEQRSKAIGVWTGVAGGGGI
                     LGMFLSALLVDVADWRWLFVLPVVLAVVALAMTLKSVPDSREHSAHSFDGIGALLSAVA
                     VIGLILVLQQGPESGWAAPATLISLIAGVMAAIGFVAWELRRRDASLLDVRLFRERGLA
                     GGSITLLAVFGVQAGIAVVLFPFFQAVLGWSGLLSTVAMMPMAVMMMMASGLAPKMAAR
                     FGARSTMTVGVALGALGLALMALFVSVEGGYLSILAGMLAMGVGMGLSMTPSTEAITSS
                     LPREKQGVASALNDVTREFGTALGVALLGALLSAGYRGAIDGRLHGIPAGTADTAREGV
                     ANAVEAARSAGSQAQDLVHAAQQSFVDGWQQAMWAGVAVMGVLLVYVAFRGPKDSASTA
                     DETETAETVAAR"
     misc_feature    complement(7361..7374)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.28"
                     /tool="antismash"
     misc_feature    complement(7743..7758)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.82"
                     /tool="antismash"
     gene            7882..9984
                     /locus_tag="ASC57_RS27125"
     CDS             7882..9984
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541908.1"
                     /locus_tag="ASC57_RS27125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AAA family ATPase"
                     /protein_id="WP_055506032.1"
                     /transl_table=11
                     /translation="MATPTGWRREGNLPAELTSFVGRTRLLANLKRHLSESRLVTVTGI
                     GGVGKSRTVLRLAHQVRAQYPDGVWFADLARLQDAGMVKHTIASALGIADQSSRATSES
                     LAEWVGTRDMLLIIDTCEHLVQGCAELINELLEAAPNLKVLATSRQSLHLPYEHVVPVP
                     PLQVPGDDPAENLFANESVQLFAARAGGVVPDFVLDEENIGSVAELCRRLDGIPLAIEL
                     AAVRLRALSVDQILALLTDRFSLLAGASRTALPRHKTLRAAIGWSHELCEPAERLLWAR
                     LSVFAGDFELDAARFVCSDSRLRAEEITDLVSGLVEKSILLIRSNHAGVRYKLIDTLAE
                     YGEEWLDKLGETERLRQRHLEYYLSLALRSEDAWSGPRQIYWFVRMRQEHDNVRMALEH
                     ALRTPGMSALALTLLSSLWFMWVCCGFAREGRVYLERALQANPGYSKERCKALWVLSYV
                     CSAQGDSVGALEAAEKCSTEAVRVGDSRAVILASKMQGTAALLQGDLQKASALLGVAIE
                     FNTDNKELNPGLLPAIVELAIVLTSQGELYEAESLLIDCLQVCRERGELSVSSYAHWAL
                     SSTRLQTGRADAALENAQESLRIKRHFHDTLGTLIALETTARIYTALEEVDVASQLLGA
                     LQENWRTAGLPMLGAPFLQLEHEKCVKELERLMGEDAYREAFTRGTNLDLDEATILALG
                     DNAASP"
     gene            complement(10001..11002)
                     /locus_tag="ASC57_RS27130"
     CDS             complement(10001..11002)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /locus_tag="ASC57_RS27130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_143078595.1"
                     /sec_met_domain="T1TS (E-value: 1.6e-86, bitscore: 287.2,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSATPAHASLPLRIPPLLCPFASEVNPHVAEASEDSFAWLLESGM
                     LRDPEILERYRSARFGWLTARAYPLVDRDMLKLLMDWCIWLFAFDDGFCESEDLSKHTA
                     LIARALPEMFRVLHDIETDQPITNVFAKSLQGLKERIAVHADRDQLDRWSTATKEYIFA
                     QVWEASNRETDIVPTPEDYIFMRRRTGAMYPVYALIDIAGRRRLAPEEWHHPDVRALTE
                     HANDLVVWDNDLFSYAKERKHDQARHNLVNVLVTHRGYSLQDALDEVASMHDEAVAEMV
                     RLRTAVEKWGSPAVLAYIQGLEHWVRGHIEYSLGSARYVHAWPAGTHWEQLS"
     gene            11308..12207
                     /locus_tag="ASC57_RS27135"
     CDS             11308..12207
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893305.1"
                     /locus_tag="ASC57_RS27135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="WP_055506034.1"
                     /transl_table=11
                     /translation="MFDPKDLYELADDVPELTDPVMLYHFDGFVDAGGAGRLALGHLLA
                     ELEHRVVATFDVDRLLDYRSRRPIMTFDTDRWVECESPELALHATKDATGTDFLILSGP
                     EPDREWELFVRAVAALVERLKVSKLVTLHGIPMAVPHTRPLGITAHASKPELVTSQSSV
                     FGRVQVPGSAAALLELRLGAMGHDAVGYAVHVPHYLSQAEYPTAAVTGLEAVTRGTGLV
                     FPMEQLHEAARRTTTEIEEQIQASTELSTAIVGLEQQYDAFAAGAERESLLAETVPMPT
                     GDELAAQFERFLAERDEE"
     gene            12227..13267
                     /locus_tag="ASC57_RS27140"
     CDS             12227..13267
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA_C3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546453.1"
                     /locus_tag="ASC57_RS27140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="WP_055506035.1"
                     /sec_met_domain="GFO_IDH_MocA (E-value: 1.1e-26, bitscore:
                     89.7, seeds: 39, tool: rule-based-clusters)"
                     /sec_met_domain="GFO_IDH_MocA_C3 (E-value: 8.1e-30,
                     bitscore: 99.2, seeds: 4226, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEIRVGLIGYGTAGAFFHAPLVAATPGLRLAAVVTRDAGRAAEV
                     AARYGAEAVPGVDELWPRCDLVVVASPNRTHVALATEALGQGLPVVIDKPAARTAEEAR
                     GLVRLAEERGLMLTVFQNRRWDGDFRTVRRLVAEGALGEVRRFESRFERWRPVPKGGWR
                     EAGGPEDVAGLLYDLGSHLVDQALLLLGPVREVYCESDVRRAGVAADDDTFVALTHESG
                     ARSHLWVSSVAALSGPRLRVLGSAGAYVKYGMDVQEERLRAGRSPGEPGFGVEDDGRWG
                     LLGTDAEQRPVPTEPGSYLDFYVGVEAALREGTPPPVDPAGSIDTLAVIEAARRSATER
                     TVVSLP"
     gene            complement(13426..14208)
                     /locus_tag="ASC57_RS27145"
     CDS             complement(13426..14208)
                     /EC_number="3.1.3.48"
                     /GO_function="GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546454.1"
                     /locus_tag="ASC57_RS27145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosine-protein phosphatase"
                     /protein_id="WP_055506036.1"
                     /transl_table=11
                     /translation="MTRWIDLEGAVNARDLGGLPTRDGGVTRHGRILRADNLQALTPAD
                     VALLVGDLKLRHVVDLRSTAEVTLEGPGPLTRVPDVTHHHLTLFAEGGRHTDVEADSID
                     IDKVLPWQERAVDEELRVTGFYFGYLRDRPDSVLAALRALSLDDGAALVHCAAGKDRTG
                     VVCALALEVAGVTREAVVEDYTATGERLELILKRLRSSATYRDDLDSRPADDHLPRARY
                     IEQFLGVLDDRFGGPMDWLGSHGWTDADTAALRARLRD"
     misc_feature    complement(14241..14255)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.94"
                     /tool="antismash"
     gene            14282..15154
                     /locus_tag="ASC57_RS27150"
     CDS             14282..15154
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC57_RS27150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_143078596.1"
                     /transl_table=11
                     /translation="MDPRALPPRLVVDPSLPPEISAELKSSPHILRMARSGRRMDPSYN
                     PAMLFVLPGFLVLMMVLLNSPGLIVAAAGSTLVILIRWLALDGPYRANKRRLRLAQEYA
                     NHYILPEDVDHPCQMLLRRAQNAAEAIISSRVNRDGLIDTIDNQVTLREEVWQIAQRLR
                     RLSAMHAEHGRIVPRELPPGMEDAFKPYGEALDAAWTSLARRVRHLEKYAKQVLKADRV
                     YHAHRRLETLAARTPDYQRLIADTVRDELANVRIKELGDQAAHVRRMFEDSILQAKLAA
                     GELFRTPLP"
     misc_feature    14732..14734
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(15228..17816)
                     /locus_tag="ASC57_RS27155"
     CDS             complement(15228..17816)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /locus_tag="ASC57_RS27155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_152992555.1"
                     /transl_table=11
                     /translation="MTGELPDKPQSRPQPHPQRWRRMPVAAPVSAGLAAAAASILAASL
                     EWDIPPVAKIGVTLAAAALVGVTTWATTEVKPRRRAPADPGSMLPDHWPPGPEHFTGRA
                     EALSELREVFSARRKAGDLAPPLVVSVYGRGGVGKSALMARFGQEVSRWFPDGRLYADL
                     RGGADAPVRPDEVLIGFLRALGVRLTTDPGGLEELRKLWLTWTKGRKLLIGLDNAHDAD
                     QVRPLIPAEPGCAVLVTSRQPLFLNGTHDTRLGVFSEAHGVELLARLAGGDRVVDDLDA
                     AKEIVRICDHLPLAINICGGRLATREQWTLRQMADRLADERRRLDQLQVAATVDKSVRA
                     SVQLSYDDCTGIQRRLLRLLSALPAPDVPGWVAGELLDISVLDGADQLEALIDAQLAEC
                     SGADQTGALRYRLHDLVRVFAAELAGQDEEERRRAAVERVLYGYRRRAEEAAQDRWPQD
                     WSKGGRRSTPEVQVRAGDWLNAERLSLLAMIDLSRRLGLWELTGGLARAFCSLCHSLRA
                     YWADWASVAELGCEAAGRTGDRRALAIALLDSAAARGGMGVRERALKESGQALEIFTEL
                     GESWWAARAMRAVGMTLFAEGNLDRAQDYLIDAIAAFKGEDDVWWMARTQRNLAEMRMA
                     ERRPEEARELLETALEVFKREGNRYSEAQTLRVYGEVLAAQARNAHREGDPRSSANLFT
                     RAGFSLDRAGEMFRQRGELWEEARCLRAAGEVGDPSNGLRELDHVRRAEEILTALGDAW
                     GVARTALSSGRVLARLGRIAEADAELRRAMRAFEELEDRWWMALSLRHLGEAHLEAGDR
                     PAAVPPLRQARDIYRSLGNEAGMRRTLELLRRAESGPPAPGGGGEGPEVR"
     misc_feature    complement(16344..16346)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(17887..19995)
                     /locus_tag="ASC57_RS27160"
     CDS             complement(17887..19995)
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893309.1"
                     /locus_tag="ASC57_RS27160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="WP_055506039.1"
                     /transl_table=11
                     /translation="MNAPKKLLKGLLTVLVLVPASLVATPASAAPPADDPEHPWRRAHW
                     PQTQPWQQDQPGDAQALRGRPGGGIAPIDPQNWENPDHMTWADYTKVPGTAWNDPAVKG
                     SKRTFKGALVLLDYPNQPFVVTQPKGSTIFGNPSAEAHDVPRDQVATFYQDFLNTPNAL
                     NKGHTIHEYWMEDSGGRYGVELTGFGPYTLSGKDHEYAMEFQGGTGCPSGDSCDKNLRT
                     EGNAAWLADIGEEAAKQFDFVFYLSAGQDESSTWQEFGMMKFPTKEDVPDEWGPPDPNL
                     PNWVKTRYVDWTSWQAGSTFWPNARFGIDSVQAESSGMAVYAHELSHIMGIADNYNNPY
                     GVPPRRAYTGIWEMLSRGSFNGPGGPHSRWLIPPTAGASMGAQHMLRNKIKLEMVDEQN
                     VLRLSRDALDDSGLVTARITARAVQPGPAGLSGVNISFDSGDKAPACDTRTDPLCDGGG
                     YDNYTVEVVDRMGTDSFTPDSGVLLAKTKNADRAPFEWVIDANPQDIGMTDYVMPDGTK
                     VPITIGDYRQLSDALFHAGTDSGSEYEYVDEANRLHFYVTDVKRDKAGVLSYTVAVRSL
                     DGAGPQRRGVRLLPAAGVPAGRDVSTCRFPLFNTGKAAPAQGQHPEDVSAYLKGDVYRL
                     KAEVQGKGWTAQLPNALAAVDFGEKELVTVNAVRAGGGDRLAKVTLTATSESDPTKTVT
                     ATCHVVGL"
     misc_feature    20017..20030
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.8"
                     /tool="antismash"
     misc_feature    20189..20204
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.09"
                     /tool="antismash"
ORIGIN
        1 ccgccggtgc tgcccgccgg gctcgtggcg ccgttccgcg tgaccgtccc cgacggcgcg
       61 ggcagcgtcg aggccaccgt caccctgccc gcggtcgacc accagttcgc cgcggggcac
      121 cggctgcggc tcgtcgtgac caccaccgac atggggtacg ccacgcccgc ccagcctgcc
      181 tcgtaccggg tggcgctggc ctcgcctgcg ctggccgtcc cgaccgtcgc gacgctggcc
      241 gccgcgccca cggggctcgc gtggtgggtg tgggcgatgc cgctggccgc cgtggtggtc
      301 gccgccgtgc tgctggcgac cggccgcacc acgcgccgcc gccgcgccgc ggcgtccgcc
      361 gcgtccgggc ccggcgtcgc gccacgggag ctccagctcg cggcggccgc ccgggaagcc
      421 ggctccgccg cgtccggcaa cggccacgtc accgcgccgg acgggaagga gacgggcgcc
      481 ggcgttcccg gcgacgggtc tccggcgccg ctggagatcg tcgggctgac caaggcgtac
      541 aggaacggcg agcgcgccgt cgacggcctg tcgttccgtg tggagcaggg gcaggtgctc
      601 ggcctgctcg gccccaacgg cgcgggcaag accaccacga tgcggatgat gatggggctc
      661 atccatcccg acgacggcga gatccgcatc ttcggcgagc cggtcacgcc cggcgcgccc
      721 gtcctgtccc ggctcggctc cttcgtcgag ggccccgggt tcctgccgca cctgtccggc
      781 cgcgacaacc tggagctcta ctgggccgcc accggccgtc ccgccgccga cgcgcggttc
      841 gacgaggcgc tggagatcgc gggtctcggc aaggcgctgg agcgggccgt acgcacctac
      901 tcccagggca tgcgccagcg gctcgccatc gcgcaggcca tgctcggcct ccccgacctg
      961 ctcgtgctcg acgagcccac gaacggtctc gacccgcccc agatcaggga gatgcgcgag
     1021 gtgctcaagc gctacgcccg cgacggccgt accgtgatcg tctccagcca cctgctcgcc
     1081 gaggtggagc aggcgtgcag ccacgtcgtg gtcatgcacc gcggacggct cgtgtccacc
     1141 ggcccggtct cgaccctgct cgcgtcggcc accaccaacg gccacggccc acggctggaa
     1201 gacgtgttcc tcgacctcat cggagagaag tgatgaccgc cgccatcggc catgcggcca
     1261 ggcaggcccc cggctacgcg cccggccgca cgctgcccct gcgggtggag ttcgtcaggc
     1321 agttcaagcg gcggcggacc ctcgtcatgt tcgggctgct gctcgccctg ccgtggatcc
     1381 tcgtgatcgc gttccagttc ggtccgcagt ccagcggcca agggcagtcc gcgctgcgca
     1441 tctccgacct ggccaccgcc ggcgggctga acttcgcggc gttcgccctg tccgtgtcgg
     1501 cgagcttcct gctggtggtg gcggtggcgc tgttctgcgg cgacacggtg tccagcgagg
     1561 ccaactggtc gtcgctgcgt tacctgctcg ccgcgcccgt gccccgcgac cggctgctgc
     1621 gccagaagct gatcgtcgcc ctgggctact ccgccatggc ggtgatctgc ctgccgctga
     1681 tggcgctgct cgccggcacg ctcgcgttcg gctggaacga cgtccaggtg cccggcaccg
     1741 gcgagatcat cccggcgctg gaggtgctgc cgcgtttcgg catcgtcatc ggctacgcgc
     1801 tggtcagcca gctcgtggtc gccgccctgg ccttcctcgt ctcgaccatg accgactctc
     1861 ccctgggggc ggtcggcggc gcggtcggcc tgtggatcgt ctgcaacatc ctgcaggcgg
     1921 tggaggcgct ggggtcgctc agggagttcc tgccgacgtt ctggaacaac gcctggctcg
     1981 acgccctcgc acccgagccc gacatgagcg gcatggcgaa gggcgcgtcc ctctcgatca
     2041 cgtacgcggc cctgttgatc gcgttcgcct tccgccgctt ccgccacaag gacgtggtca
     2101 gctagacggg caggcggtgc gccgctcgcc gaggatttgg ggggaaggta tgggggcccg
     2161 ggtcgttgat ctgtcagcag gcccgggtcg ttgatctcga caggcccgag tcgttgatct
     2221 tggcgttggc gacgatgtcg gccttgccgt tcgctccggc gagctgctcc caccagccct
     2281 gacgccaggc ttcgcagccg gcgggcacgc cgttcgcgac gtcggccgtc tgctgaacca
     2341 gcccgaacag cgctccgagc aaccagccgc tgcggtggat gacggcgaac gcggcgtgtt
     2401 ggagggggtc tgaggtgcgg tcatgaaagt gctccctgtc cttccgccgc gccattgggg
     2461 tgcggcgata gccgctgggg gtgcggcggt agcgccgggc cctgggagcg tgcggcggta
     2521 gtgccgcacc ctgggagcgt gcgacggtag cgccgcgtgc ggcagtgcgc cggctctcct
     2581 ttccgtccgc gcgcgatgtc gatctgcacc ggcgcgttcg tgctggccgc cgccgggcga
     2641 gccggtgccg agccccacga gcgggtggcc acggtgagcc cgcccgcgac gacgctcata
     2701 gccctggccc tggccctggc cctggtggtg acgggcacca cgccgatcgc ggtggacgtc
     2761 gcggtgcagg atgcaggcgg cgtgcgcgtg cctgagggcg tccagcaccc tgatgccgtc
     2821 tgctcgctcg tcgtggccgc cgatgccgcc ggcccggccg atggaaccag cagatggggc
     2881 agaacgcgaa ctggggtgag gtgctgccgc accacgggcg gtgcggcggt agcgccgcgt
     2941 gcggcagtgc gtgggctctc ctttccgccg cgccatggga ggtgcggctg ctctggtgct
     3001 cagctgacga agcgcacggc ggtgcccgtg gcgatgcccg tgacggcgcc cgtgacggta
     3061 cgcgtggtgg tacgcgtgac ggtgcatgtg acggtgcgcg tgacggtgca cgtgggggtg
     3121 tgcatggtgg tgcgcgtggc ggtgcgcgcg gtggtgcgcg tggcggtgcg cgcggtggtg
     3181 cgcgtggcgg tggttgcctt tgcggcgagt aacggggtgc tggggctgcg gggcgggcgg
     3241 ttgggcgcgg gcagggctca ggcgcggcgg gctgcccaga cggtgcgctc ggtgcgtacg
     3301 gcgaggtcgt cgcggcgtag gatgctgtgc gggctttcgg tgtcgaggag ctcgtcgagt
     3361 gcggcgaggt cttcggggct gagcgccggt gcggcggcgc cgcgtacgcg ctgcaggctg
     3421 ccctgggcgt agcggccgat ggcttcgctg ccgtcgcctt cgatgttcac ggtgatggtg
     3481 cgcacgtctt cgacggtgaa accggcggcg gccagcatcc gtccccagtc agcgccccgg
     3541 tgccggacgt gctcggcgtg gaagccgtca gtcgccctgt ggcagcgttc ttcgagaccg
     3601 ggcctgttct cgggggcgtc ggcgggcagg aagcggggga agccgtccag ctcgacgacg
     3661 gcgaacaggc cgcccggggc gagcaggtcg tggacgccgg ccagggtgcg gtcggggtgg
     3721 gccatgtggt gcatcgaggc cgaggcccac accaggtccg gcgtcccgag atcgggccag
     3781 tcagcggcgt cgaggtcggc ctgcacggtg cgcacgcgct cctccacccc gcgggcgcac
     3841 gccttctcgc gcaggcgctg caggtacccg gtcgaggtgt cgacggcggt gacgtgcgcc
     3901 tcagggaaac gatcgaggag cgcgaaggtg cccgcccccg tgccacagcc gagatccacg
     3961 atgtggcgcg gctcggcctt gagcggcagc caggcggtga tgaacgccag gtgctcggcg
     4021 agcacctcgg catccaggtc gaggatctcc gcctggctgc catcatcgtg cgccgggtgg
     4081 tggccaccgt cgccgtgacc atggccaccg tcaccgtaac catggccgtg gccaccgtca
     4141 ccgtaaccat ggccgtggcc gccatcaccg taaccaaggc cgtggccgcc atcaccgtaa
     4201 ccaaggccgt ggccaccgtc accgtaacca aggccgtggc cgccatcacc gtaaccaagg
     4261 ccgtggccac cgtcaccgta accaaggccg tggccaccgt caccgtaacc aaggccgtgg
     4321 ccaccgtcac cgtaaccaag gccgtggcca ccgtcaccgt aaccaaggcc gtggccgtct
     4381 tcggcatgac ggtggttgtg gccgccttcg ccgtgcgggg tgtgctggtg tgtttgggtc
     4441 atggcgacac cgtaagacgc tcatgcgcct gaagcacagc cacttgcgat atacgcaaga
     4501 agctcgcatc acctgctgcc cctcacgcaa ggtcccgcgt gtgccggtcc tctggccccg
     4561 tcattcgtag ggggtctgag cgtcgccgcg gtcgccggcc tcggcgacgt cgcgttggtg
     4621 gccgcggcgg gcatcgcggt cgaagatgcc caggatctcg cacggcccac cctcagcgcc
     4681 gatcgcgtgc ggcatcatcg tggggaactc ggcggcctgg ttggtctcga tgcggaaccg
     4741 gcggtgcccg agcatgagga tggcggtgcc ggacaggacc acaagccatt cgcggccggg
     4801 gtgggcgcgc atgcgggcag ggttgtcggg cggcgggtcg gtcatccgct gacgcatcac
     4861 gctcatgccg ggatcgccct ttatcggcca gcgcatcaga ccgtgggcgc cgtcgatcat
     4921 cgggctgatg acgacgtcgt cggcggcgtt ctccaccagt tggtccaggc tggtgtccag
     4981 ggcgcgggcg agggtgacga gctggtccag ggcgaggcgg cgccggccgt tctcgatgcg
     5041 gctgagcgag gactggctga ggttggcacg ggtggccagt tcctccaggg accagccctg
     5101 ggcgacccgc agggcgcgga tccgtttgcg tacgaggctt tccaactgcc catcttcttg
     5161 cgtcataagc aacatgctat gcccctaccg caaggctgac ttaacgtcga ctacgggtgg
     5221 ctcggaacag gggccacgca cgacgaaagg gcgcgacatg tctgcgcaca ttcgccgagc
     5281 cctcctggcc gccggggacg acggcgcggc ccccgctgca gcacggagcg aggacgccac
     5341 cgtctgaacc cctccgccgc tgagccgcgc ggccgcaccc ggcggtcctc gcggtgagcg
     5401 agtacgggcc ttcccctcat cgattcggcg accggccctg ctccggcgct gatcccgtct
     5461 gccctcttct gaaaggaaag catgagtacg aaccagcctc cccaggcggc accggcgagc
     5521 ggcgggggca cactggacga gcgcgagctg cgcgcgatcc tgatcgccgt ctccgtcgcg
     5581 ctgatggccg tcatcgcgtc ggtgtccggg ctgaacgtcg cccagaccca cctggctgtg
     5641 gagttcggcg ccccgcagaa cgccgttctg tggatcatca atgtctacac cctggccctg
     5701 gccgcgctgc tgctgccgct cggcgccatc ggtgaccggc tgggccgtaa gcccatgctg
     5761 atcaccgggc tgaccgtctt cggtgccgcc ggcatcgtcg cggggctggc tccgtcgacc
     5821 gaggtgatga tcgccgcgcg cctggccggc ggtgtgagcg ctgcgatgat catgccgatc
     5881 acgctggccg tcatcacgag tacgttccct gaggagcagc ggagcaaggc gatcggcgtg
     5941 tggacgggtg tcgccggggg cggcggcatc ctgggcatgt tcctgtccgc gctgctggtc
     6001 gacgtcgccg actggcgctg gctgttcgtc ctgcccgtgg tcctggccgt cgtggctctg
     6061 gcgatgacgc tgaagtcggt gcccgactcc cgcgagcact ccgctcattc cttcgacggc
     6121 atcggcgcct tgctgtccgc cgtcgcggtg atcggtctga tccttgtcct gcagcaaggc
     6181 cccgagagcg gctgggccgc cccggcgact ctgatcagcc tgatcgccgg tgtcatggcc
     6241 gccatcgggt tcgtggcctg ggagctgcgc cgccgcgacg cctcactgct ggacgtgcgc
     6301 ctgttccgtg agcgtggcct ggccggcggc tcgatcacgc tgctggcggt cttcggggtc
     6361 caggcgggta tcgccgtggt tctcttcccg ttcttccagg ccgtgctggg ctggtcgggg
     6421 ctgctgtcca ccgtggcgat gatgccgatg gccgtcatga tgatgatggc gtccggcctg
     6481 gcccccaaga tggccgcgcg cttcggcgcc cgctccacca tgaccgtggg cgtcgccctg
     6541 ggcgccctcg gcctggcact gatggctctg ttcgtctccg tcgaaggcgg atacctctcc
     6601 atcttggccg gcatgctcgc catgggcgtc ggcatgggcc tgtcgatgac gccctccacc
     6661 gaggccatca ccagctccct gccgcgcgag aaacagggcg tcgcctccgc actcaacgac
     6721 gtcacccgcg agttcggcac cgctctcggt gtcgccctgc tcggcgcgct gctgtcggcc
     6781 ggctaccgcg gcgccatcga cggcaggctg cacggcatcc cggcggggac cgcggacacc
     6841 gcgcgagaag gcgtcgccaa cgccgtcgag gctgcgcgta gcgcgggctc gcaggcacag
     6901 gacctggtcc acgccgctca gcagtccttc gtcgacggct ggcagcaggc catgtgggca
     6961 ggcgtcgccg tcatgggcgt gctgctcgtc tacgtcgctt tccgcggccc gaaggactcg
     7021 gcttccaccg cggacgagac ggagaccgca gagaccgtcg ccgcccgatg accgcgtaac
     7081 acccgggccg gatcatccgt caggcatcgc ggtaccgcga tgcctgacgg cccagccgcc
     7141 ggaagggctg ccggaccgcg gaccgtccct ggccgaagac cggcccggcc tgctcgtcat
     7201 cgcggacaag ggcttcgcct cccccgtgtt cgagacgcct tccgggacgc cgagtcactc
     7261 cgcccgtcct tcaaagcggg agaagcgccg caagggcgag tctccgccga agccggtacg
     7321 gcgcctgatc gagtccgtca gtcacatgcc cgaggccggc tcgatctgga acaacatggt
     7381 ggacggacct tcgagaccgt tgccgtccgc gtcgcctgtc ggggagagcc gagggacggg
     7441 gggctgccgg cccgtacgag tggccgcggc gggggcgacg gcggtcatgc ccggacggcc
     7501 gggagcggcg gcgattcccg ctgcggagag ccggaggaac cgagcggagg gccgctgaac
     7561 gcgggcaccc ggcgggcgag cgctcctgga ggtgcgggcc gcgatgacgg gctggggagg
     7621 gcgtcggccg ggtgggagaa ggtcagctcc agtggggcgg cttgttctcc aggtagaagg
     7681 tgtcggggtt gtcgtcgcgc cagtcgcccc agccgaggtc ggtgtcgtcg gacgtctgct
     7741 ccgggagaaa atcgttcacg cctttcatgc tacctagagg gagaaagcca atcctcaggt
     7801 ctcggtggct gcccagacag gcgggtttcc cggcggtacg atgcgagggc aggtgtccgc
     7861 ctatcccccg tgaggctgcc catggccacc ccgacaggtt ggcggcgaga aggcaatcta
     7921 ccggcggagc tcacaagctt cgtgggacgt acgcgcctac tcgccaatct gaagaggcac
     7981 ctgagcgagt cgcggctcgt gaccgtgacc ggcatcggcg gtgtgggcaa gtcccgcacg
     8041 gtgctgcggc tggcgcacca ggtgcgtgcg cagtaccccg acggcgtctg gttcgccgac
     8101 ctggcgcgcc tgcaggacgc cggcatggtg aagcacacga tcgcgtcggc gctcggcatc
     8161 gccgaccagt cctcgcgcgc cacctccgag tcgctcgccg aatgggtggg cacgcgcgac
     8221 atgctgctca tcatcgacac ctgcgagcac ctggtgcagg gctgcgccga gctgatcaac
     8281 gagctgctgg aggccgcgcc caatctgaag gtgctggcca cgagccggca gtcgctgcat
     8341 ctgccgtacg agcacgtggt gccggtgccg ccgctgcagg tgccgggcga cgaccccgcc
     8401 gagaacctgt tcgcgaacga gtcggtgcag ttgttcgcgg ccagggcggg cggggtggtg
     8461 cccgacttcg tcctggacga ggagaacatc ggctcggtgg ccgagctgtg ccgcaggctc
     8521 gacggcatcc cgctggcgat cgagctggcg gcggtgcggc tgcgcgcgct gtcggtcgac
     8581 cagatcctgg ccctgctgac cgaccggttc agcctgctgg ccggggccag ccgcacggcg
     8641 ctgccgcgac acaagacgct cagggcggcg atcggctgga gccacgagct gtgcgagccc
     8701 gcggagcgcc tgctgtgggc gcgcctgtcg gtgttcgcgg gcgacttcga gctggacgcg
     8761 gcacgtttcg tctgctccga ctcgcgcctg cgcgccgagg agatcacaga cctggtgtcc
     8821 ggcctggtcg agaagtccat cctgctcatc aggagcaacc acgcgggcgt ccgctacaag
     8881 ctgatcgaca ccctggccga gtacggcgag gagtggctgg acaagctggg cgagaccgag
     8941 cgtctgcgtc agcggcatct ggagtactac ctgagcctcg cgttgcgcag cgaggacgcc
     9001 tggtcggggc cgcggcagat ctactggttc gtacggatgc ggcaggagca cgacaacgtc
     9061 cgcatggcgc tggagcacgc gctgcggact cccgggatga gcgccctggc gctgaccctg
     9121 ctgtcgtcgc tgtggttcat gtgggtgtgc tgtggtttcg ccagagaggg gcgggtctac
     9181 ctggaaaggg cccttcaggc gaacccgggg tacagcaagg agcgctgcaa ggcgctctgg
     9241 gtgctgtcgt acgtgtgcag cgcgcagggc gacagcgtgg gcgcgctgga ggcggcggag
     9301 aagtgcagca cggaggcggt ccgggtgggc gactccaggg ccgtcatcct cgcctcgaag
     9361 atgcagggca cggcggcact gctccaggga gatctgcaga aggcgagcgc gctgctgggc
     9421 gtggcgatcg agttcaacac cgacaacaag gagctgaacc cgggcctgct gcccgcgatc
     9481 gtcgaactgg ccatcgtgct cacctcccag ggcgagctgt acgaggccga gtcactgctg
     9541 atcgactgcc tgcaggtgtg cagggaacgc ggcgagctgt cggtcagctc gtacgcgcac
     9601 tgggctctgt cctcgacccg cctgcagacc ggccgggcgg acgcggccct ggagaacgcg
     9661 caggagagcc tgcggatcaa gcgccacttc cacgacaccc tgggcacgct catcgcgttg
     9721 gagacgaccg ccaggatcta cacggctctg gaggaggtcg acgtcgcgag ccagttgctg
     9781 ggcgcgctgc aggagaactg gcggacggcc ggtctcccca tgctgggcgc tccgttcctg
     9841 cagctcgaac acgagaagtg cgtgaaggag ctcgagcgcc tcatgggcga ggacgcttac
     9901 cgggaggcct tcacccgggg aacgaacctc gacctggacg aggcgacgat tctcgccctc
     9961 ggcgacaacg ccgcctcacc ctaggccgtc tgcggaggtc tcaggagagc tgctcccagt
    10021 gggtgcccgc cggccaggcg tgcacgtagc gcgcgctgcc gagggagtac tcgatgtggc
    10081 cccgtaccca gtgttcgagg ccctggatgt acgcgagcac cgcgggcgac ccccacttct
    10141 cgaccgcggt gcggagccgt accatctcgg cgacggcctc gtcgtgcatc gaggcgacct
    10201 cgtcgagggc gtcctgcaag gagtagccgc ggtgggtgac caggacgttc accaggttgt
    10261 gccgtgcctg gtcgtgcttg cgctccttgg cgtaggagaa caggtcgttg tcccagacga
    10321 caaggtcgtt ggcgtgctcg gtgagcgccc ggacgtcggg gtggtgccat tcctcgggcg
    10381 ccagccgccg gcgtccggcg atgtcgatga gcgcgtagac ggggtacatg gctccggtcc
    10441 tgcgccgcat gaagatgtag tcctcgggcg tcggcacgat gtcggtctcc cggttggacg
    10501 cctcccacac ctgcgcgaag atgtactcct tcgtggcggt gctccaccgg tcgagctggt
    10561 cgcggtcggc gtggacggcg atgcgctcct tgagcccctg cagtgatttg gcgaagacgt
    10621 tggtgatcgg ctgatccgtc tcgatgtcgt gcaggacgcg gaacatctcg gggagcgccc
    10681 tggcgatgag cgcggtgtgc ttgctgaggt cttctgactc gcagaagccg tcgtcgaacg
    10741 cgaacagcca gatgcaccag tccatgagca gcttgagcat gtcgcggtcg acgagggggt
    10801 aggcgcgcgc ggtgagccag ccgaacctgg cgctgcggta gcgctccagg atttcgggat
    10861 ctcgcagcat gccggattcg aggagccagg cgaagctgtc ctcgctggcc tcggcgacgt
    10921 gcgggttcac ctcgctcgcg aaggggcaca gcagcggcgg gatccggagg gggaggctgg
    10981 cgtgcgctgg ggtagcagac atgccataga tgatcgcaat gatcaaccct attgcctata
    11041 tagcgggatg accgctttta tttacctcgc ttgattggtc tttatgaagc gagccaatct
    11101 cccgggccgg gcattcgcct cgttccggcc cctgacggtg gccggaggcg atgacgtggt
    11161 gcgcggcctc tcagcctggt gacggtccgg cggctctggg gcttgtgggg ttggcggggg
    11221 ttgggggtcg gtagggtgcc gtgcgcggaa tcgttctgac tcgccgtagg ttctggagaa
    11281 gagtacctag ggaaaggctg tccaaccgtg ttcgacccga aggatctcta cgagctcgcc
    11341 gacgacgtgc ccgagctgac cgatccggtg atgttgtacc actttgacgg gtttgtcgac
    11401 gcgggcggcg ccgggcgcct cgcgctgggc cacctgctcg ccgagctgga gcaccgcgtt
    11461 gtggcgacgt tcgatgtgga ccggctgctg gactaccggt cgcgtcggcc gatcatgacg
    11521 ttcgacaccg acaggtgggt ggagtgcgag agccccgagc tcgcgctgca cgcgacgaag
    11581 gacgcgacgg gcaccgactt cctgatcctc agcgggcccg agcccgacag ggagtgggag
    11641 ctgttcgtca gggcggtggc cgcgctggtg gagcggctga aggtgagcaa gctcgtgacc
    11701 ctgcacggca tccccatggc cgtgccgcac acgcgcccgc tcggcatcac cgcccacgcc
    11761 agcaagccgg agctcgtcac ctcgcagtcg agcgtgttcg gccgcgtgca ggtgccgggc
    11821 agcgcggcgg cgctgctgga gctgcggctc ggcgcgatgg gccacgacgc cgtcgggtac
    11881 gccgtgcacg tgccccacta cctgtcgcag gcggagtacc cgacggccgc cgtgaccggg
    11941 cttgaggcgg tcacccgggg caccggcctg gtgttcccga tggagcagct gcacgaggcg
    12001 gcccgccgga cgacgactga gatcgaggag cagatccagg cctcgaccga gctgtccacc
    12061 gcgatcgtcg gcctggagca gcagtacgac gcgttcgccg ccggggcgga gcgcgagagc
    12121 ctgctcgccg agacggtgcc gatgccgacg ggcgacgagc tggccgccca gttcgagcgc
    12181 ttcctggccg agcgggacga ggagtgacac ccgttagcct gtggggatga gtgagatccg
    12241 ggtcgggctg atcggttacg gcacggcagg ggcgttcttc cacgccccgc tcgtcgccgc
    12301 gacacccggg ctgcggctgg ccgccgtggt gacgcgggac gccggccggg ccgccgaggt
    12361 cgcggcgcgt tacggcgccg aggcggtgcc gggcgtggac gagctgtggc cgcgctgcga
    12421 cctggtcgtg gtggcctccc cgaaccggac gcacgtcgcg ctggcgacgg aggcgctcgg
    12481 ccaggggctg cccgtggtca tcgacaagcc ggcggcccgc accgccgagg aggctcgggg
    12541 tctggtgcgg ctggccgagg agcgcgggct catgctgacg gtgttccaga accggcggtg
    12601 ggacggcgac ttccgcacgg tccggaggct ggtcgcggag ggggcgctcg gcgaggtgcg
    12661 gcggttcgag tcgcgcttcg agcggtggcg tcccgtgccg aagggcggct ggcgggaggc
    12721 cggcggcccc gaggacgtcg ccgggctgct ctacgacctc ggcagccacc tggtggacca
    12781 ggcgctgctg ctgctcggac cggtccgcga ggtctactgc gagagcgacg tcaggcgtgc
    12841 cggggtggcc gccgacgacg acacgttcgt cgcgctgacg cacgagagcg gcgcccgctc
    12901 acacctgtgg gtcagctcgg tggcggcgct gtccgggcca cggctgcgcg tgctgggctc
    12961 ggcgggcgcg tacgtcaagt acgggatgga cgtgcaggag gagcgcctgc gggcggggcg
    13021 ctcgcccggc gagcccgggt tcggcgtgga ggacgacggc cggtggggcc tgctgggcac
    13081 ggacgcggag cagcgccccg tcccgacgga gccggggtcg tacctcgact tctacgtcgg
    13141 ggtggaggcg gcgctgcggg aggggacgcc gccgcccgtc gacccggcgg gctcgatcga
    13201 cacgctggcg gtgatcgagg cggcccgccg gtcggcgacg gagcggacgg tcgtcagcct
    13261 gccctgaccc cctcggagtc cgtcgtgcgg tgtccgtcgt gcggtgtccg tcgtggcggg
    13321 cccaggtgcg tggtgcggtc ggccctcgca ggggcggtcg gggcgatggt cagttgtcgc
    13381 agggggcggg cgggggcggt ggcttggcgc gggaagcggg cactgtcagt cgcggaggcg
    13441 ggcgcgcagg gcggcggtgt ccgcgtccgt ccagccgtgg ctgccgagcc agtccatcgg
    13501 cccgccgaag cggtcgtcca gcacgccgag gaactgctcg atgtagcggg cgcggggcag
    13561 gtggtcgtcg gcgggccgcg agtcgaggtc gtcgcggtac gtggcgctgg agcgcagccg
    13621 cttgagtatc agctccagcc gctcgccggt ggcggtgtag tcctcgacga cggcctccct
    13681 ggtcaccccg gccacttcga gcgcgagcgc gcacacgacg ccggtgcggt ccttgccggc
    13741 ggcgcagtgg acgagggcgg ccccgtcgtc gagcgacagc gcgcgcagcg cggcgagcac
    13801 cgagtcgggc ctgtcgcgca ggtagccgaa gtagaagccg gtgacgcgca gttcctcgtc
    13861 cacggcgcgt tcctgccagg gcagcacctt gtcgatgtcg atgctgtcgg cctcgacgtc
    13921 ggtgtgcctg ccgccttcgg cgaacagggt caggtggtgg tgggtcacgt cgggcacgcg
    13981 ggtgaggggt cctggccctt cgagggtcac ctcggcggtg gagcgcaggt ccacgacgtg
    14041 ccgcagtttc aggtcgccca cgaggagtgc cacgtcggcg ggagtgagcg cctggaggtt
    14101 gtcggcgcgc aggatccggc cgtggcgggt gacgccgccg tcgcgggtgg ggagtccgcc
    14161 caggtcgcgc gcgttcaccg cgccttccag atcaatccat ctcgtcatct ctttcaccgt
    14221 aacgacctta tatgtccgcc aaatctcagc gaattcatcg ttcataacgc agcatgaacg
    14281 tatggatcca cgagcgctgc ctccacggct ggtggtcgat ccatcgctgc ccccggagat
    14341 ctccgccgag ctgaagtcca gcccgcacat cctgcggatg gcgagatccg gccggcgcat
    14401 ggacccctcc tacaaccccg ccatgctgtt cgtgctcccc ggcttcctgg tgctgatgat
    14461 ggtgctgctc aactcccccg gcctgatcgt cgccgccgcg ggcagcacgc tggtcatcct
    14521 gatcaggtgg ctggcgcttg acggcccgta ccgggcgaac aagcgcaggc tgcggctggc
    14581 gcaggagtac gcgaaccact acatcctccc cgaggacgtc gatcacccct gccagatgct
    14641 gctgcgccgg gcgcagaacg cggccgaggc gatcatctcc tcccgggtga acagggacgg
    14701 cctgatcgac accatcgaca accaggtgac tttacgggag gaggtgtggc agatcgccca
    14761 gcgattgcgc cgcctgtcgg ccatgcacgc cgagcacggc aggatcgtcc cccgcgagct
    14821 gcccccgggc atggaggacg cgttcaagcc gtacggcgaa gcgctggacg ccgcgtggac
    14881 gtcgctggcc aggcgggtgc ggcacctgga gaagtacgcc aagcaggtgc tcaaggccga
    14941 ccgggtctac cacgcgcacc ggcggctgga gacgctggcg gcgcgcacgc ccgactacca
    15001 gcggctgatc gccgacaccg tgcgggacga gctggcgaac gtccgcatca aggagctggg
    15061 cgaccaggcg gcgcacgtcc gcaggatgtt cgaggacagc atcctgcagg ccaagctggc
    15121 ggcgggcgag ctgttcagaa ccccgctccc ctgacctccg tccagaaagc cgttcacctg
    15181 acctccgtcc ggaaagccgt tcacctgacc tccgtccgga aagccgttca cctgacctcc
    15241 ggcccctctc cgcccccgcc cggcgccggc ggtccggact ccgcgcgccg cagcagctcc
    15301 agggtcctgc gcatgccggc ctcgttgccg aggctgcggt agatgtccct ggcctgccgc
    15361 agcggcggga cggcggccgg ccggtcgccc gcctccaggt gcgcctcgcc gaggtggcgc
    15421 aggctcagcg ccatccacca gcggtcctcc agctcctcga acgcgcgcat ggcccggcgc
    15481 agctccgcgt cggcctcggc gatgcggccg agcctggcca gcacgcgccc ggacgacagc
    15541 gccgtgcgcg ccacccccca ggcgtcgccc agcgcggtca ggatctcctc cgccctgcgt
    15601 acgtggtcga gctcgcgcag cccgttggac gggtcgccga cctcgcccgc ggcgcgcagg
    15661 cagcgggcct cctcccacag ctcgccgcgc tgcctgaaca tctcgccggc ccggtcgagg
    15721 ctgaagccgg cccgggtgaa caggttggcg gaggagcggg ggtcgccctc gcggtgggcg
    15781 ttgcgggcct gggcggcgag cacctcgccg tacacgcgca gcgtctgcgc ctcggagtag
    15841 cggttgccct cgcgcttgaa cacctccagc gcggtctcca gcagttccct ggcctcctcg
    15901 gggcggcgtt cggccatgcg catctcggcc aggttgcgct gggtgcgggc catccaccag
    15961 acgtcgtcct cgcccttgaa ggcggcgatg gcgtcgatga ggtagtcctg ggcgcggtcg
    16021 aggttgccct cggcgaacag cgtcatgccg accgcgcgca tggccctcgc ggcccaccag
    16081 ctctcgccca gctcggtgaa gatctccagc gcctgccccg actccttcag cgcccgctcg
    16141 cgcacgccca tgccgccccg cgcggcggcg ctgtcgagca gcgcgatggc gagggcgcgg
    16201 cggtcgccgg tccgcccggc ggcctcgcag cccagctcgg cgacgctcgc ccagtccgcc
    16261 cagtacgccc gcagcgagtg gcacagcgag cagaacgccc tggccaggcc cccggtcagc
    16321 tcccacagcc cgagccggcg ggataagtcg atcatggcga gcagggagag gcgttcggcg
    16381 ttgagccagt cgccggcccg gacctgcacc tcgggggtgg agcggcggcc gcccttgctc
    16441 cagtcctgcg gccagcggtc ctgcgcggcc tcctcggcgc ggcggcggta gccgtacagg
    16501 acgcgctcca cggcggccct gcgccgctcc tcctcgtcct ggccggcgag ctcggcggcg
    16561 aacaccctga ccaggtcgtg cagccggtag cgcagggcgc cggtctggtc ggcgcccgag
    16621 cactcggcga gctgggcgtc gatcagcgcc tccagctggt cggcgccgtc gaggacggag
    16681 atgtcgagca gctcccccgc cacccagccg ggcacgtcgg gcgcgggcag cgcgctgagc
    16741 aggcgcagca ggcggcgctg gatgccggtg cagtcgtcgt agctgagctg gacggaggcg
    16801 cgcacgctct tgtcgacggt ggcggcgacc tggagctggt cgaggcggcg gcgctcgtcg
    16861 gccagccggt cggccatctg gcgcagcgtc cactgctcgc gggtggccag gcgtccgccg
    16921 cagatgttga tcgccagcgg caggtggtcg cagatcctga cgatctcctt ggcggcgtcg
    16981 aggtcgtcga cgacgcggtc gccgcccgcg agcctggcga gcagctccac gccgtgggcc
    17041 tcgctgaaga cgccgagcct ggtgtcgtgg gtgccgttga ggaacagcgg ctggcgggac
    17101 gtgaccagca ccgcgcagcc cggctcggcg gggatgaggg gacgcacctg gtcggcgtcg
    17161 tgggcgttgt cgagcccgat gagcagcttg cggcccttgg tccaggtgag ccagagcttg
    17221 cgcagctcct ccagcccgcc cgggtcggtg gtgaggcgga cgccgagcgc ccgcaggaac
    17281 ccgatcagca cctcgtcggg ccgcaccggg gcgtcggcgc cgccgcgcag gtcggcgtac
    17341 aggcggccgt cggggaacca gcgggagacc tcctggccga acctggccat gagcgccgac
    17401 ttgccgacgc cgccccggcc gtacaccgag acgacgaggg gtggggcgag gtcgccggcc
    17461 ttcctgcggg ccgagaacac ctctcgcagc tcgctgagcg cctcggcgcg gccggtgaag
    17521 tgctcggggc cgggcggcca gtggtcgggc agcatcgagc cggggtcggc cggggcccgg
    17581 cgccggggct tgacctcggt ggtggcccag gtggtgacgc cgacgagcgc ggcggcagcg
    17641 agggtcacgc cgatcttggc gaccggtggg atgtcccatt cgagcgaggc cgccaggatg
    17701 ctcgcggcgg cggcggcgag gcccgcggag acgggcgcgg ccacgggcat cctgcgccac
    17761 cgctgcggat gcggctgggg ccgcgactgc ggcttgtcag gcagctcccc tgtcaaagaa
    17821 aacccccttc gggggtgatt caagccgatg gggtacggcc tgcgccagcc gtaccccatc
    17881 gttcatctaa aggccgacga cgtgacaggt cgccgtcacc gtcttggtcg gatcgctctc
    17941 cgaggtggcg gtgagcgtca ccttggcgag cctgtcgccg ccgcccgcgc ggacggcgtt
    18001 gaccgtgacg agctccttct cgccgaagtc cacggcggcc agggcgttgg gcagctgggc
    18061 ggtccagccc ttgccctgca cctcggcctt gagccggtag acgtcgccct tgaggtacgc
    18121 gctgacgtcc tcggggtgct gcccctgcgc gggggcggcc ttcccggtgt tgaacagcgg
    18181 gaacctgcag gtggacacgt cgcgcccggc cgggacgccg gcggcgggca gcagccgcac
    18241 gccgcgccgc tgcgggccgg cgccgtcgag cgagcggacg gccaccgtgt acgacaggac
    18301 gcccgccttg tcgcgcttga cgtcggtgac gtagaagtgc aggcggttgg cctcgtccac
    18361 gtactcgtac tcgctgccgg agtcggtgcc ggcgtggaag agcgcgtcgg acagctggcg
    18421 gtagtcgccg atcgtgatcg gcaccttggt gccgtcgggc atcacgtagt cggtcatgcc
    18481 gatgtcctgc gggttggcgt cgatcaccca ctcgaacggg gcccggtcgg cgttcttggt
    18541 cttggccagc aggacgccgg agtcgggggt gaaggagtcg gtgcccatgc ggtcgacgac
    18601 ctcgacggtg tagttgtcgt agccgccgcc gtcgcacagc gggtcggtcc tggtgtcgca
    18661 ggcgggggcc ttgtcgccgc tgtcgaagga gatgttcacg ccggacagcc cggccgggcc
    18721 cggctggacg gcgcgggcgg tgatcctcgc ggtgaccagt ccggagtcgt cgagcgcgtc
    18781 acgcgagagc cgcaggacgt tctgctcgtc caccatctcc agcttgatct tgttgcggag
    18841 catgtgctgg gcgcccatgg acgcgcccgc ggtgggcggg atgagccagc gcgagtgcgg
    18901 gccgccgggg ccgttgaagc tgccgcggct gagcatctcc cagatgccgg tgtaggcccg
    18961 gcgcggcggc accccgtacg ggttgttgta gttgtcggcg atgcccatga tgtggctcag
    19021 ctcgtgggcg tagacggcca tgccggagct ttcggcctgc accgagtcga tgccgaagcg
    19081 ggcgttgggc cagaaggtgg acccggcctg ccacgacgtc cagtccacgt agcgggtctt
    19141 cacccagttg ggaaggttgg ggtcgggcgg gccccactcg tccggcacgt cctccttggt
    19201 ggggaacttc atcatgccga actcctgcca ggtggacgac tcgtcctggc cggcgctgag
    19261 gtagaagacg aagtcgaact gcttggcggc ctcctcgccg atgtcggcga gccaggcggc
    19321 gttgccctcg gtgcgcaggt tcttgtcgca gctgtcgccg gacgggcagc cggtgccgcc
    19381 ctggaactcc atggcgtact cgtggtcctt gccggacagc gtgtacgggc cgaagccggt
    19441 cagctcgacg ccgtaccggc cgccggagtc ctccatccag tactcgtgga tggtgtgccc
    19501 cttgttgagg gcgttcgggg tgttgaggaa gtcctggtag aacgtggcga cctggtcgcg
    19561 gggcacgtcg tgcgcctcgg cgctggggtt gccgaagatc gtcgagccct tgggctgggt
    19621 cacgacgaag ggctggttcg ggtagtcgag cagcaccagc gcgcccttga aggtgcgctt
    19681 ggagcccttg acggcggggt cgttccaggc ggtgccgggg accttggtgt agtccgccca
    19741 cgtcatgtgg tcggggttct cccagttctg ggggtcgatc ggcgcgatgc cgccgccggg
    19801 gcggccgcgc agtgcctgcg cgtcaccggg ctggtcctgc tgccacggct gggtctgcgg
    19861 ccagtgggcg cgccgccagg ggtgctcggg gtcgtcggcg gggggtgctg cggaagcggg
    19921 ggtggccacg agactcgccg gaaccaggac gagtaccgtc agcaacccct tgagcagctt
    19981 cttcggggca ttcacgccct gacggtatag acgttcttga cggagatcat cgatcagttg
    20041 tagggaagac ggataccgac tcttccgaca gatgttgaaa tgcgcatgta gacgggtgtg
    20101 actgtggcgg acctccagcg gaccgtggac gacctcgccg cgcggctggg ccgcccgctg
    20161 ctgctggagg accgggtgca gcgtgtcgtc gcctacagcg agcagagcgg cccgatggac
    20221 gacatcaggc gcgagtcgat cctgcgcagg cggaccacgc ccgaggtgcg ggagtggctg
    20281 cgagccgccg gcatccagga ctcccccggc cccctgcgca cccccggcgc gccgcacctg
    20341 cgcctgctgc cgcgcgtgtg cgtcccgctg cggcacgccg acgggctgct cggctacctg
    20401 tggttcatcg acgccgggcc gcccatgacc gacgcgcacg tcgccgaggc cgccgccgcc
    20461 gcgcccggcc tcgcgctcgc cctcttccac gagagcctgg ccaccgggct cgcctcccac
    20521 agggagctgg aggcggtgac cgagctgctg cgcggcgagc cgggcgccgc cgccaagctc
    20581 atcgaggccg ggaccgtgcc gcaggcgctg ccggtgaccg tccaggtggc caggccgctc
    20641 gcggccgaac cgggcgaggg gctgcggctg gcgctcgaac aggggctgct cacgctgcgc
    20701 cgccgcctcg gcggccacca cccgctccac ctcgtccggt acgaccacgc ggtgctcctg
    20761 acgtccgggc cgccggtctc gcccgacgag ctgcacgccg ccatgccggt gccggtggtc
    20821 gtcggcatcg gacggcccag gccggagctg tcggaggcgg cgggctcgta cgaggaggcc
    20881 aggcacgcgg cggaggtggc cgcccgggtg ccggggctgg gccggtcggc cgagtgggcg
    20941 cggctcggcg tctaccggat gctcaccggc ctgccggaac gggagctgca ccccgggctc
    21001 ga
//
