LOCUS       NZ_BBZG01000001        21008 bp    DNA     linear   CON 12-AUG-2024
DEFINITION  Nonomuraea pusilla strain NBRC 110462, whole genome shotgun
            sequence.
ACCESSION   NZ_BBZG01000001
VERSION     NZ_BBZG01000001.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nonomuraea pusilla
  ORGANISM  Nonomuraea pusilla
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft Genome Sequence of Nonomuraea sp. TP-A0861, a Producer of
            Myxochelin A
  JOURNAL   Genome Announc 3 (6), e01430-15 (2015)
   PUBMED   26659677
  REMARK    DOI:10.1128/genomeA.01430-15 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746196)
  AUTHORS   Hosoyama,A., Kimura,A., Ichikawa,N. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2015) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, Biological
            Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.8
            Genome Coverage                   :: 107x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417755.1-RS_2024_08_12
            Annotation Date                   :: 08/12/2024 01:46:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,307
            CDSs (total)                      :: 8,242
            Genes (coding)                    :: 8,159
            CDSs (with protein)               :: 8,159
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 21 of 83
            Pseudo Genes (incomplete)         :: 72 of 83
            Pseudo Genes (internal stop)      :: 5 of 83
            Pseudo Genes (multiple problems)  :: 15 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:25:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3190119
            Orig. end                         :: 3211127
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBZG01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21008
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="13"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21008
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21008
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11008](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11008
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(119..135)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.32"
                     /tool="antismash"
     misc_feature    568..577
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    1962..1976
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     misc_feature    2580..2594
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            2867..4030
                     /locus_tag="ASC57_RS44615"
     CDS             2867..4030
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025160.4"
                     /locus_tag="ASC57_RS44615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HXXEE domain-containing protein"
                     /protein_id="WP_063792010.1"
                     /transl_table=11
                     /translation="MKTTGAKTRQTERAGQTPRTDRRRQDTAGLPTAQHRQAGQPRVAG
                     VAGVAGVAGVAGVAGVAGVAGVAGLMRSARIGVTAGLALTATRGETAAGPPKTAGPAET
                     AGLGREMEGRVSSAVTWGLLAAWAVHDAEELATMAGWARRARPRLERLFPQVPWQRLEV
                     SQRQVTTAIALMGGVMAGASALGARTGGRSPVFQAALAGFGAHGVVHLAQAAVTRGYTP
                     GVVTSPLVVIPFSLWAWRRLRESGVAVGAGGATAAGLAALPAVVAGVHVLAHALSGHTD
                     ARHGSQTPGKNHTDAQDADQPDGASRHGDGWHGDAWQGDAWQGDAWQGDAWQGDAWQGD
                     AWHGGQACGKDRRADVPRGDRPGREGRFGKVGGGVSRVRRFCRERWF"
     gene            complement(3990..4820)
                     /locus_tag="ASC57_RS14165"
     CDS             complement(3990..4820)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1249: carbamoyl phosphate synthase small subunit"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /locus_tag="ASC57_RS14165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="WP_082534883.1"
                     /transl_table=11
                     /translation="MRITVIEHEAEAGLGYFAGWLGLDCEVHRPYLGDEVPARAADGLI
                     VLGGAAAAWEDDRHPWLPATRDLMRRSVEDGTPTLGICLGAQLMTLACGGVVERGAHGL
                     EVGACEVTPLPAAAADPLLSRLDGVATAVQYHQDAMTRLPDGAVPLVTGSPYPNQAYRL
                     GEAAWGVQFHPEATPEIFVSWTRDSGLDAAEELNAAVKAAEDALVATWRPVARAFADAV
                     RTTAGARTEAGVRADEGVRADAGGRADAGVRADAGVRSDTGVRTTARDRTGARD"
     gene            4961..5815
                     /locus_tag="ASC57_RS14170"
     CDS             4961..5815
                     /NRPS_PKS="Domain: Abhydrolase_1 (19-268). E-value:
                     1.2e-17. Score: 56.3. Matches aSDomain:
                     nrpspksdomains_ASC57_RS14170_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541541.1"
                     /locus_tag="ASC57_RS14170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_055503269.1"
                     /sec_met_domain="PF00561 (E-value: 3.7e-13, bitscore: 47.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 7.6e-18, bitscore:
                     63.6, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVDEVGGLRIATFGTGPRVIIAVHGITASLMAWAAVGRRLPEEW
                     SLVAMDLRGRGHSASLPGPYGLSRHAEDVLRVADHVGAGPDAVLTGHSMGAYVAVLAAA
                     EREFERVVLVDGGLPMPLPPGVDPDETMAAMLGPALARLRRTYPSAEAYVDFFKAHPAF
                     AGRGWSAEVEEYVRYDLTGPEGALRSRAVPEAVREDGRWLLVESERIGAALSAITSPLY
                     LLRAPRGLLDQDTGMLPDALTGEWTARLPGLRDEVVPDCNHYTILFDERCVATVVERLT
                     SGK"
     aSDomain        5018..5764
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ASC57_RS14170_Abhydrolase_1.1"
                     /evalue="1.20E-17"
                     /label="ASC57_RS14170_Abhydrolase_1.1"
                     /locus_tag="ASC57_RS14170"
                     /protein_end="268"
                     /protein_start="19"
                     /score="56.3"
                     /tool="antismash"
                     /translation="VIIAVHGITASLMAWAAVGRRLPEEWSLVAMDLRGRGHSASLPGP
                     YGLSRHAEDVLRVADHVGAGPDAVLTGHSMGAYVAVLAAAEREFERVVLVDGGLPMPLP
                     PGVDPDETMAAMLGPALARLRRTYPSAEAYVDFFKAHPAFAGRGWSAEVEEYVRYDLTG
                     PEGALRSRAVPEAVREDGRWLLVESERIGAALSAITSPLYLLRAPRGLLDQDTGMLPDA
                     LTGEWTARLPGLRDEVVPDCNHYTILF"
     CDS_motif       5222..5272
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ASC57_RS14170_0001"
                     /evalue="1.00E-03"
                     /label="NRPS-te1"
                     /locus_tag="ASC57_RS14170"
                     /protein_end="104"
                     /protein_start="87"
                     /score="11.7"
                     /tool="antismash"
                     /translation="VLTGHSMGAYVAVLAAA"
     gene            complement(5891..6607)
                     /locus_tag="ASC57_RS14175"
     CDS             complement(5891..6607)
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541540.1"
                     /locus_tag="ASC57_RS14175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PaaI family thioesterase"
                     /protein_id="WP_055503270.1"
                     /transl_table=11
                     /translation="MTTAALQTMLTVPQRFRGPAGVANGGWIAGTMAGALNGGRSAVEV
                     TLHAPTPLETELRLEHVANSVSLHHDETLLVEAIPVAEELDAPRFVPFNDAARAEANFV
                     GWKGGHPFAECFACGQREPGDGLRIFPGRVEGTDLVAAGWRVPFSVADEDGVPPSIVGA
                     VLDCVTGWAHFGPGESALLGRLALSIHRPVHPGGQYSVVARPTGQDGRKMYGESAIYEA
                     DGTLVANARATWIAPR"
     misc_feature    6883..6896
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            7308..7853
                     /locus_tag="ASC57_RS14180"
     CDS             7308..7853
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889399.1"
                     /locus_tag="ASC57_RS14180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AAA family ATPase"
                     /protein_id="WP_236714704.1"
                     /transl_table=11
                     /translation="MILTGLPGAGKTTLLRRLYGLTGAEGSPAVSGPAVVIDSAQSRLR
                     WRGRLDRLPKPLRTAAVFATHVTRIARALAAGHAVVAHNRGCAPYVLRGFAWLARRHGA
                     GFHLLLLDASPEAALAGQQARGRVVAPATFVRHRRRWEALLTRARAGDPSPAASAHVMT
                     RADAARLTAIHFDATESR"
     gene            complement(7893..9254)
                     /locus_tag="ASC57_RS14185"
     CDS             complement(7893..9254)
                     /EC_number="6.3.1.-"
                     /GO_function="GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151975.1"
                     /locus_tag="ASC57_RS14185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glutamine synthetase family protein"
                     /protein_id="WP_055503271.1"
                     /transl_table=11
                     /translation="MDRQQEFVLRTLEERDIRFIRLWFTDVLGFLKSVAIAPAELEGAF
                     AEGIGFDGSAIEGFARVYESDMLAKPDPATFQILPWRSETPGAGRIFCDILMPDGSPSH
                     ADPRWVLKRTLAKCADMGFTFYTHPEIEFFLLKNRPEPGQRPEPIDSGGYFDHTPHSSG
                     HDFRRQAIMMLESMGISVEFSHHEGAPGQQEIDLRYADALTTADNIMTFRLVMKEVALE
                     QGIWASFMPKPFTEHPGSGMHTHMSLFEGDRNAFYEAGADYQLSKTGRSFIAGLLKHAA
                     EITAITNQWVNSYKRLWGGAEAIAGQGGEAPSYICWGHNNRSALVRVPMYKPAKAGSTR
                     IEFRSLDSACNPYLAFALILAAGLKGIEQGYELPPAAEDNVWRLTSAERRALGIQPLPQ
                     SLDEAVSVMERSELVAETLGEHVFDFFLRNKRAEWRDYRRTVTEFELGRYLPVL"
     gene            9436..9981
                     /locus_tag="ASC57_RS14190"
     CDS             9436..9981
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541536.1"
                     /locus_tag="ASC57_RS14190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="EF-hand domain-containing protein"
                     /protein_id="WP_055503272.1"
                     /transl_table=11
                     /translation="MAIDLLNHKLDRAFDHIDANGRGLVAREDLLGLGARILVGFGESP
                     TSVTGKSLVESFDGIWSALTGVLGHDVDASLTRDEFREGMAAAFVTGGHYDPVFRPATV
                     AVAELCDGDGDGRIGRPEFRTMLTAFGTAYDDVDAAFDRLDRTGRGTLGVEDLVVAARE
                     FYGGDDPNASGNWLFGPL"
     misc_feature    complement(9464..9479)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.1"
                     /tool="antismash"
     gene            10001..11008
                     /locus_tag="ASC57_RS14195"
     CDS             10001..11008
                     /GO_function="GO:0010333 - terpene synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541535.1"
                     /locus_tag="ASC57_RS14195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="terpene synthase family protein"
                     /protein_id="WP_055503273.1"
                     /sec_met_domain="T1TS (E-value: 5.7e-48, bitscore: 162.0,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTVMSTAVSTAVSTAMSTARTALRSVLKPLLPLPRRGGPAALAL
                     LPLTERVPALARPCRMHPSVYAIEAAVIDWARRTGLRADPGVGYHRMAGRAFAEFDAAA
                     AALFAQWLTWLFHLDDELDERGGPLEVFQGLLAGAASHPLEAAFADLWRVTSARMSDRW
                     RARFAVNLRRQHEACRAEADNRASGRMPAIEEYPVLRRGTVGPYLFDLVEPCLRVEVPA
                     WVHECESWQELVDACSDVTAWCNDVASRPKERDDAHNLVVLAARQLGLAERDAVAWVLD
                     RIARRCQDMRRAARRLPLHDLPPAAARDVSKVACAYLAAPRAHLDWLLESARYA"
     gene            complement(11049..12011)
                     /locus_tag="ASC57_RS14200"
     CDS             complement(11049..12011)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541534.1"
                     /locus_tag="ASC57_RS14200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5937 family protein"
                     /protein_id="WP_055503274.1"
                     /transl_table=11
                     /translation="MTVTWRLNAEDVARIRFAFSPMWELVASLRTLQDPSRQSIHLPWI
                     KRVRPRLAALDLDELLALVPPTGYLADFLTPPPDTPLPDFAAELDRVRSTPPEQARAEA
                     AIVRGTDPAVLRRFAADPAAGVARVADALLAYWEECFAEFWPRVYALLERDVLRRSRQL
                     AREGARELFAGLDPAVVWTGEQLVIDRPWCATGGLHGDGLVLVPTAFYWPSVAVMSAPY
                     QSMLVYPVLGVGTLWEEGPPPAPGALAALIGRNRARILLALAEPSTTTALAARMALTPG
                     AVSQHLGVLSAGGLTVGMRVGKQVVYRRTQTGDMLAGRA"
     gene            12071..13291
                     /locus_tag="ASC57_RS14205"
     CDS             12071..13291
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889321.1"
                     /locus_tag="ASC57_RS14205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_082534885.1"
                     /transl_table=11
                     /translation="MSTATSSGPSPRVVKKPAFVRSKLGGLPRPFWALWTGTLVNRLGS
                     VVVPFTGVYLTQERGLSVTAAGLVIGVYGVGSLVSQILAGVLTDRVGRRATLAGGMLAT
                     AAALLALGYSTSMPAILASMAVLGVVTDVYRPASNAMVADLVDPEERPRAYGLLFWGIN
                     IGYALGMTAGGWLAERGFTWLFWIDALSSVLFAVLVWRAVPETRPAGREAAGGGFGEVL
                     RDRLMVAFTLVVLGDALMYMQTVSMLPVAITRVAGLTPGQLGVAMALNGVLIVVVQPLV
                     SDWLGRRDPARTFALGLAVMGLGFAMTAFVTSTLWLAVTIVVWTAGEIVTAGIAGSILA
                     TLAPPHLRGRYSGLFGLAWSAAMVLAPALGGPLLEIGPEALWFTIGGVGLVSAAGMLAL
                     GPAIRRR"
     gene            13431..14876
                     /locus_tag="ASC57_RS14210"
     CDS             13431..14876
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889319.1"
                     /locus_tag="ASC57_RS14210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_055503275.1"
                     /transl_table=11
                     /translation="MLTFTALGPFQAWSDGAPLDLGGQRQRAVLARLLVAGGRAVPVAT
                     LIDELWPGSPPPQALSTIQGYVSRLRRALEPGRAPREEAEVLVSAPPGYALRAATDQVD
                     AWRFEYLVKSDGPPAQVWAAMDEALELWRGPALAEFSDLAWAATEAGRLEELRLIAVER
                     RAAAGLALGRATSLVADLEAHASAYPLREEGWRLLALALYAMGRQGDALAALRRARAVW
                     REELGLDLSAALQRLEGDMLAQRLEEPAAPAATAPEASGTLRVLVADDQALVRAGLRVM
                     LESEAGVALAGEAADGEEAIARARETGADVVLLDIQMPHLDGLAAARRILAQERPPKVI
                     MMTTFGSDDNLYAALRAGVSGFLLKTSPPEQILAAIRAAAAGDALIDPAVTTRIIAAFA
                     GRTDPASPPALAGLSDAQLDVLKLVARGLTNRQIGQTLALPEHEVAAAVSALLEHLGLF
                     DRAQLVMAAYESGLVTPGGMSQK"
     misc_feature    14882..14902
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: medium, score: 27.53"
                     /tool="antismash"
     gene            14929..16701
                     /locus_tag="ASC57_RS14215"
     CDS             14929..16701
                     /EC_number="6.3.5.1"
                     /GO_function="GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853434.1"
                     /locus_tag="ASC57_RS14215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD+ synthase"
                     /protein_id="WP_055503276.1"
                     /transl_table=11
                     /translation="MAQLRIALAQTNPAVGDLTANAERLVAWTRDAADRGAHLAVFTEM
                     FLTGYPVEDLVLRTSFVEASIRTLEETAARLEREGLGHLPVVVGYVDRAGLAPRVGQPK
                     GAPLDAAALLHEGRVVTRTAKHHLPNYGVFDEYRYFVRGDRLPIFRLHGVDVAIAVCED
                     LWQDGGPVSVVGQAGAGLLVVPNASPYEKEKDDVRLELVARRAREAGCALAYANQVGGQ
                     DELVFDGDSIVVDASGALVARAGQFREELLVVDLDLPVSTAATGIFPYDAGDGTTITVD
                     RFVVSSEPVESYAPEPGVIAERLDLPAEVYSALVLSVRDYVAKNGFQSVILGLSGGIDS
                     ALVATIASDAIGPSRVHVALMPSRYSSDHSVSDADELVRRQGIHARVVPIADIVTAYEK
                     EIDLSGLAAENLQARVRGMILMGLSNEHGHLVLTTGNKSELATGYSTLYGDSAGGFAPI
                     KDVPKTMVWELARWRNAHTCPGFLLDAERPIPENSIVKEPSAELRPDQRDTDSLPPYEV
                     LDRLLDDYVEKDMGSRELIAAGHDPALVARVIRLVDLAEYKRRQYPPGPKITPKNFGRD
                     RRLPITNRWRESAG"
     gene            16929..18569
                     /gene="ctaD"
                     /locus_tag="ASC57_RS14220"
     CDS             16929..18569
                     /EC_number="7.1.1.9"
                     /GO_function="GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ctaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546514.1"
                     /locus_tag="ASC57_RS14220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="WP_256256884.1"
                     /transl_table=11
                     /translation="MTSPLAPVHARTGAVLSKWLSSTDHKVIGYMYLIASFVFFLIGGI
                     MALIIRAELAQPGLQLVSNQQYNQLFTMHGTIMLLLFATPLFAGFANVIMPLQIGSPDV
                     AFPRLNAASLWLFVFGGIIAVSGFFTSGGAADFGWFAYAPLNSKTYSHLIGCDLWIMGL
                     VLSGLGTILGSVNFFTTIICMRAPGMTMFRLPIFCWNTLLTSMIVLMAFPVLAAALLVL
                     ELDRRFGTVVFEAPNGGPILWQHLFWFFGHPEVYIIALPFFGIVTEVLPVFSRKPIFGY
                     IGLVAATIAIAGLSMTVWAHHMFGTGQVLLPFFSFMTFLIAVPTGVKFFNWIGTMWRGH
                     LTFEPPMLFSVGFLATFLLGGLTGVILASPPLDFHITDTYFVVAHFHYVVFGTVVFAMF
                     SGFYFWWPKFTGKMLDKTLGTWHFWLLFIGFHTTFLIQHWIGAIGMPRRYADYSPADGF
                     TDLNIISSIGALILGISTLPFLYNVWHTARYAPRVTVDDPWGYGNSLEWATSCPPPRHN
                     FTGIPPIRSERPAFDLHYPHSQWREVESP"
     gene            complement(18640..19296)
                     /locus_tag="ASC57_RS14225"
     CDS             complement(18640..19296)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC57_RS14225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_152992382.1"
                     /transl_table=11
                     /translation="MRPLVAALVATAVVTAACANQSTDASLRHYQPNEGTNYDLKGVLH
                     VRNAFLLSGATTSAAPPAASPSSPPASPPSASGPASPSGQPTGQGGGQDALFAVLINSA
                     AKPDTLERITVEGGGQVQLPGPIELPPNQPVGTGNRPIASVSGVKGGPVAMTFSFRNAG
                     DLRLLLPVMRRTGIYASLTPSPAGPPVPSPAGTAPVATPSQPSPPSSRPASPAPS"
     gene            complement(19487..20089)
                     /locus_tag="ASC57_RS14230"
     CDS             complement(19487..20089)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1224: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547732.1"
                     /locus_tag="ASC57_RS14230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_055503279.1"
                     /transl_table=11
                     /translation="MTIQESGTTRPAGRPRSEKAERAIIEATLDLIGEGMGVSELSIEA
                     IASRAGVGKTTIYRRWSNKEDLVVDALSTLKSPLPPLSGTSVREDLIALLDAMRRESGQ
                     ARSRCVMNIAMSESDRYPRLMERFFKRAIEPRREALRAVIERGIASGELRPDLDVELGT
                     AVLSGTMLWYTKWGPAGDLAPDLAERVVDAALHGLTP"
     misc_feature    complement(20804..20818)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.3"
                     /tool="antismash"
     misc_feature    complement(20828..20842)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.37"
                     /tool="antismash"
ORIGIN
        1 cttgaccagc atgagggcgt ccctgacgcg ggaggcgaag cgccacgccc cggccagcac
       61 ctcctcgtcg cgctcggaca gcagcccctc ggccgccgcc gcgcgcagcg cctccagcgt
      121 gcgcgtggtg cgcagggacg gcaggtccgc cgcgtgcctg agctggagca gctgcgccac
      181 ccactcgacg tccgacagcc cgccggggcc gagcttggtg tgcagcgcgg ggtcggcgcc
      241 cctgggcagc cgctccgcct ccatgcgggc cttcagccgc ctgatctcgc gcaccgcgtc
      301 gtcgccgagc ccgccgcgcg ggtaccgcag ctcgtcgatc atggacgtga agtcggcgcc
      361 cagctcctcg tcccccgcgg agaagcgggc ccgcagcagc gcctgcgcct cccacggcga
      421 cgaccagcgc gcgtagtagg cgcggtacga ggccagcgtg cgcaccagcg gaccctggcg
      481 gccctccgga cgcaggtcgg gatcgaccat cagcggcggg tcgggcgcgg gcagcgcgag
      541 cagcctgcgc agctcgttgg cggcggcgaa ggcggcctcg gcggcctccc gctcggccac
      601 ccccgggcag ggcgagtgga cgaacatgac gtcggcgtcg ctcgcgtacg agcactccag
      661 cccgccgagc cggcccatgg cgacgaccgc gaacctggtc acgcccgcga ccgggcgcag
      721 cgcggcgtcc agcgtcgcct gcagggtgac gtcgttgagc cgcgacagcg cctcgccgac
      781 cgtctcgatg tcggcgaggc cggagacgtc ggccaccgcc gtgcggaaca gctccctgcg
      841 gcgcagcgcc cgcacggcgg cgacggcgct ctcgacgtcg ccgccgtgcc ttgacacggc
      901 cgtccgggcc tcgttcagca gcgacgcggc gggacgcacg gcgaggtcgg cgtcggagcc
      961 gagcatggcc accgcctcgg gggcgtgcat gagcaggccg gtggcgtacc tgctggtgcc
     1021 cagcacgcgc gccatcctcg ccgcgaccgc ggtctcgtcg cgcagcagcc gcagatacca
     1081 gggcgtggcg ccgagcctgt cgctgacctg gcggaagccg agcagcgcgg cgtccgggtc
     1141 gggcgtgtcg gcgaaccagc cgagcatgac cggcagcagc gtgcgctgga tcgccgcgcg
     1201 cctggacacg ccgctggtga gcgccgcgat gtggcgcagc gccccggcgg ggtcgacgta
     1261 gccgagcgcc tccagccggg ccgccgccgc cgacgccgac agccgcgact ccgactcggg
     1321 cagccgcgcc accgcctgca gcagcggccg gtagaacagc ttctcgtgca gccgcctggc
     1381 ctccctggcg tggcgccgcc accgggccgt gaactcgccc accgggtcgc cctgcaggcc
     1441 cagcgcccgg cccagtcggc gcagcgcggc cgggtcctgg ggcaggacat gggtgcggcg
     1501 cagccggtgg agctggagca ggtgctccac gccgcgcagg aacgcgtacg cctccgccat
     1561 ggcgcgggag tcgtcacggc cgacgtaccc gcctcgcgac agggcggcca gcgcgggcag
     1621 cgtgcccctg cggcgcagca gcgggtcgag ccggccgtgg acgagctgca ggagctgcac
     1681 ggcgaactcg atgtcgcgca gcccgccggg gcccagcttg atctgccgct ccccctcggc
     1741 ccggacgtgc tcctcgacgc ggcggcgcat ggcctgcacg tcctcgacga agtgctcgcg
     1801 ggtggccgcc gtccagacga ggtcgtcgac ggcctggacg tacgactggc cgaggtccat
     1861 gtcgcccgcc accgggcggg ccttcagcag cgcctggaac tcccacgtct tggcccagcg
     1921 gcggtagtag gccaggtggc tggccagcgt gcggacgagc gggcccgatc ggccctcggg
     1981 gcgcaggccg gggtcgacct cccacagcgc gccctccggg gtgggcgcgg tgcaggcgcg
     2041 catcatggcc tgggccagcc gggtcgcggc gcgcagggcc ttggcctcgt cggcgccctc
     2101 gcgcggctcg gccacgaaga ccacgtccac gtcgctgatg tagttgagct ccctggcgcc
     2161 gcacttgccc atgccgatga cggccaggcg tacgtcggcg tcgtcgacct cggcgcgggc
     2221 gacggcgagc gcggcctcca gcgcggcccc cgcgaggtcg gacagctccg ccatgacggc
     2281 gaggagctcg gcctcgccgg tcaggtcgcg ggcggccagg tgcatgaggt gtccccggta
     2341 gccgacgcgc agggcggaca gcgcgtcctc gcgggacgcg cgcggcagcg ggtctgcggg
     2401 gtcggcgccg acggcgcgca gcagcgccgt gcgggggtcg tccagctcag gttgggcgag
     2461 cagggcgagc tggcccgggt ggcgtaccag gtggtcgccg agcgcggcgc tgacgccgag
     2521 gacggcgagc accctgccgc gcagggcggc gtcggaccgc agcaggccgg gcacggccgg
     2581 gtcgcgctcg gccaggcggg tcagcgagag cagcgcctgg tcggggtcgg cggtgtcggc
     2641 gagcgcgtcg agcaggtcgt cgagcgcggg cgctgactgc tcgacgagcc cggcggcccg
     2701 cgcggcgtcg gcgaagccga gcctggcgag ccgcgccgcg gtggattcga tcctcggcac
     2761 ccctccatct tgacagcagt cgccgggcgg gccctaaagt gaaacgcaac gttgcgttgc
     2821 gtttcgggcg catcggcatc ggcgtacggc ttcggggagg ggcacgatga agacaaccgg
     2881 ggcgaagacc aggcagaccg aacgagcagg tcagacgccg cgcaccgacc ggagaaggca
     2941 ggacaccgct gggctgccca ccgcccagca caggcaggcc gggcagccca gggtggccgg
     3001 ggtggccggg gtggccgggg tggccggggt ggcgggggtg gccggggtgg ccggggtggc
     3061 cggggtggct gggctgatgc ggtccgcccg gatcggggtg accgccgggc tcgcgttgac
     3121 tgccacgcgt ggagagacgg ccgccgggcc gccgaagacc gccggacctg cggagacggc
     3181 tgggctcggg cgagagatgg aggggcgggt ctcctccgcg gtgacgtggg ggctgctggc
     3241 cgcctgggcg gtgcacgacg cggaggaact ggccacgatg gcggggtggg cgcgccgggc
     3301 ccggccccgc ctggagcggc tgttccccca ggtgccgtgg cagcgcctgg aggtgtccca
     3361 gcggcaggtg accacggcca tcgcgctgat gggcggcgtg atggcgggcg cgtcggccct
     3421 cggggccagg accggcggcc gcagcccggt cttccaggcg gcgctggccg gcttcggcgc
     3481 gcacggcgtc gtccacctgg cgcaggccgc tgtgacgcgc ggctacacgc cgggtgtcgt
     3541 gacgtcgccg ctggtggtga tccccttctc gttgtgggcg tggcggcggc tgcgcgagag
     3601 cggcgtcgcg gtgggggcgg gcggcgcgac ggcggccggc ctggccgcgc tgcccgcggt
     3661 ggtggcgggc gtccacgtac tagcccacgc cctgtccgga cacacggacg cccggcacgg
     3721 cagccagacg cccggaaaga accacacgga cgcacaggac gccgaccagc cggacggagc
     3781 aagccgacac ggggacggat ggcacgggga cgcgtggcag ggagacgcgt ggcagggaga
     3841 cgcgtggcag ggagacgcgt ggcagggaga cgcgtggcag ggagacgcgt ggcacggcgg
     3901 ccaggcgtgc gggaaggacc ggcgcgctga cgtgccgcgc ggcgaccggc cgggtcggga
     3961 ggggcggttc gggaaggtgg ggggcggggt cagtcgcgtg cgccggttct gtcgcgagcg
     4021 gtggttctga cgccggtgtc gcttctgacg cccgcgtcgg ctctgacacc cgcgtccgcc
     4081 ctgccaccgg cgtccgccct gacgccctcg tcggccctga cgcctgcttc ggttctggcg
     4141 cccgcggtgg ttcggacggc gtccgcgaag gctcgtgcca ccggtcgcca ggtcgcgacg
     4201 agggcgtcct cggcggcctt gacggccgcg ttcagttcct cggccgcgtc caggccggag
     4261 tcccgcgtcc aggagacgaa gatctccggg gtggcctccg ggtggaactg gacgccccag
     4321 gcggcctccc ccagccggta ggcctggttc gggtacggcg agccggtcac gaggggcacc
     4381 gcgccgtcgg gcaaccgggt catggcgtcc tggtggtact gcacggcggt cgcgaccccg
     4441 tccaggcgcg acagcagcgg gtcggcggcg gcggcgggca gcggggtgac ctcgcacgca
     4501 ccgacctcca gcccgtgggc gccgcgctcc acgacgccgc cgcaggccag ggtcatgagc
     4561 tgcgccccga ggcagatgcc gagggtgggc gtgccgtcct cgaccgagcg gcgcatcagg
     4621 tcgcgggtgg cgggcagcca ggggtggcgg tcgtcctccc aggcggcggc cgcgccgccg
     4681 agcacgatca gcccgtccgc ggcccgcgcg ggcacctcgt cgcccaggta cggccggtgc
     4741 acctcgcagt ccagccccag ccagcccgcg aagtacccga gcccggcctc ggcctcatgc
     4801 tcgatcaccg tgatgcgcat aaggcgtaat ttatcgggat gagagggcga aaggggggaa
     4861 gaggggaagg tcttggtcgc cagggcggcg gcccatcacc cgcgcgccca tcccccaccg
     4921 gcccctctcc ctcgggggca tctgtcccta agatccggct atgagagttg atgaggtcgg
     4981 cgggctgcgc atcgccacat tcgggacggg tccgcgggtc atcatcgccg tacacgggat
     5041 caccgcgtcg ctcatggcgt gggccgccgt ggggcggcgg ctgcccgagg agtggtcact
     5101 ggtcgccatg gacctgcgcg ggcgcggcca cagcgcctcg ctgcccggcc cgtacgggct
     5161 gtcccgccac gccgaggacg tcctgcgcgt ggccgaccat gtcggcgccg ggcccgacgc
     5221 cgtgctcacc gggcactcca tgggcgcgta cgtggcggtg ctggccgcgg cggagcggga
     5281 gttcgagcgc gtcgtcctcg tggacggcgg cctgcccatg ccgctgcctc ccggcgtgga
     5341 cccggacgag acgatggcgg ccatgctggg ccccgcgctg gcgcggctgc gccggacgta
     5401 cccgtcggcg gaggcgtacg tcgacttctt caaggcgcac cccgccttcg cgggccgggg
     5461 ctggagcgcg gaggtggagg agtacgtccg ctacgacctg accgggcccg agggcgcgct
     5521 gcgctcgcgc gccgtgccgg aggcggtgcg cgaggacggc cgctggctgc tggtggagtc
     5581 ggagcgcatc ggggcggcgc tgtcggccat cacgtcgccg ctgtacctgc tgcgggcgcc
     5641 gcgcggcctg ctcgaccagg acaccggcat gctgcccgac gccctcaccg gcgagtggac
     5701 ggcgcgcctg cccgggctca gggacgaggt cgtgcccgac tgcaaccact acacgatcct
     5761 gttcgacgaa cgctgcgtcg ccacggtcgt ggagcggctc acgtcgggga agtgacgacc
     5821 ggccgcggac gaacgacagg ccgcggcggg gacctgcccg ccgcggccct cgaagaagtg
     5881 acggagcgcg tcagcgagga gcgatccagg tggcgcgggc gttggccacc agcgtgccgt
     5941 cggcctcgta gatggcgctc tcgccgtaca tcttgcggcc gtcctggccc gtggggcggg
     6001 ccaccacgga gtactggccg ccggggtgca cggggcggtg gatgctcagc gcgaggcggc
     6061 cgagcagcgc cgactcgccg gggccgaagt gcgcccagcc ggtgacgcag tcgagcacgg
     6121 cgccgacgat cgacggcggc acgccgtcct cgtcggccac ggagaacggc acccgccagc
     6181 cggcggccac gaggtcggtg ccctcgacgc gaccggggaa gatgcgcagg ccgtcgccgg
     6241 gctcacgctg accgcaggcg aagcactcgg cgaaggggtg gccgcccttc cagccgacga
     6301 agttggcctc ggcgcgggcg gcgtcgttga acggcacgaa gcgcggcgcg tccagctcct
     6361 cggccacggg gatggcctcg accagcagcg tctcgtcgtg gtgcagcgac accgagttgg
     6421 cgacgtgctc caggcgcagc tcggtctcca ggggcgtggg cgcgtgcagc gtgacctcga
     6481 ccgccgatct gccgccgttc agcgcgcccg ccatggtgcc cgcgatccag ccgccgttgg
     6541 cgactcctgc cgggcccctg aagcgctggg gaacggtcaa catggtctgc agggcagccg
     6601 tcgtcatgcc acaccctcca atatcgccgc acaggacgag aagtccggaa acatcattga
     6661 gagcagccga tattactgga ctccgggttt accaagatgc cgggggtcag gaaggccgct
     6721 ctgtacgtac gtacataaca ccccggaagg ggcatttact tcccacccct gggtcatccg
     6781 gcaccacggc tttcaccggg aggctaggca ccgcgcatca cggggatgtg actcgggggt
     6841 cagaagcaac gaaaatcggc cccacaccgg ggtgcacccg aggtgaggcc gatcaagcca
     6901 ccagccgtac tttatcccga gattccccta ccccgcgccc tttccgcagt ctcgcgcggg
     6961 aaagacgatc acgacttagg ctggcgccac acggagagga cggcgcattg cggggcgaga
     7021 cgatcccagc cgggcagcca ccggcggcga aggtgatgcc ggcgacgggc tggaccctga
     7081 cgacggaggg gccccatcca gacaggacgc ggacggacaa gacccggaca gacataaccc
     7141 gggcggccaa gactggggcg gacgggccgc gagcggaggg gacacccgca gacgggacgc
     7201 gggcagacgg gccgcaagcg cacaggactg acagggcgga cgggacgcgg gcggaggagc
     7261 gcccggcgcc ggggacgccg ctgctgcgct acccgccgcg gtcgctggtg atcctcacgg
     7321 gcctgccggg cgcgggcaag accacgctgc tgcgccgcct gtacggcctc accggcgccg
     7381 aagggtcccc cgccgtctcg ggcccggccg tcgtgatcga ctccgcgcag tcgcgcctgc
     7441 gctggcgggg ccgcctcgac cggttgccga aaccgctgcg caccgccgcc gtgttcgcca
     7501 cccacgtcac ccgcatcgcc cgcgccctgg ccgcgggcca cgcggtggtc gcgcacaacc
     7561 gcggctgcgc gccgtacgtg ctgcgcggct tcgcctggct ggcccgccgt cacggcgccg
     7621 gcttccacct gctcctcctg gacgcctcgc cggaggcggc gctcgccggc cagcaggccc
     7681 gcggccgggt cgtcgccccg gcgacgttcg tccgtcaccg ccgccgctgg gaggcgctgc
     7741 tgacgagggc cagggcgggc gacccgtccc cggcggcgtc cgcgcacgtc atgacccgcg
     7801 ccgacgccgc ccgcctcacc gccatccact tcgacgccac cgaatcccgc tgaggccgcg
     7861 cgaggtcctc gggtctcagc ggagagaggc ggtcacagca ccggcaggta gcggcccagc
     7921 tcgaactcgg tgacggtgcg gcggtagtcg cgccactcgg cgcgcttgtt gcgcaggaag
     7981 aagtcgaaga cgtgctcgcc cagcgtctcg gcgaccagtt cgctgcgctc catgacgctg
     8041 acggcctcgt cgagcgactg cggcagcggc tggatgccga gggcgcggcg ttcggcgctg
     8101 gtcagcctcc agacgttgtc ctcggcggcc ggcgggagct cgtacccctg ctcgatgccc
     8161 ttcaggccgg cggcgaggat cagggcgaag gcgaggtacg ggttgcaggc ggagtcgagc
     8221 gagcggaact cgatgcgggt ggagccggcc ttggccggct tgtacatcgg cacccggacg
     8281 agggcggagc ggttgttgtg gccccagcag atgtacgagg gcgcctcgcc gccctgcccg
     8341 gcgatggcct cggcgccgcc ccagagccgc ttgtacgagt tcacccactg gttggtgatg
     8401 gcggtgatct cggcggcgtg cttgagcagg cccgcgatga acgaccggcc ggtcttggag
     8461 agctggtagt cggcgccggc ctcgtagaag gcgttcctgt cgccctcgaa cagcgacatg
     8521 tgggtgtgca tgccggagcc ggggtgctcg gtgaacggct tgggcatgaa cgaggcccag
     8581 atgccctgct ccagcgcgac ctccttcatg accaggcgga aggtcatgat gttgtcggcg
     8641 gtggtgagcg cgtcggcgta gcgcaggtcg atctcctgct ggccgggggc gccctcgtgg
     8701 tggctgaact cgacggagat gcccatcgac tccagcatca tgatcgcctg gcggcggaag
     8761 tcgtggcccg agctgtgggg cgtgtggtcg aagtagccgc cggagtcgat cggctcgggc
     8821 cgctggcccg gctccggacg gttcttcagc aggaagaact cgatctcggg gtgggtgtag
     8881 aaggtgaagc ccatgtcggc gcacttggcg agcgtgcgct tgagcaccca gcgggggtcg
     8941 gcgtgggagg gggagccgtc gggcatcagg atgtcgcaga agatgcgtcc agcgccgggg
     9001 gtctcgctgc gccagggcag gatctggaag gtggccggat cgggcttggc gagcatgtcg
     9061 gactcgtaca cgcgggcgaa gccctcgatg gccgagccgt cgaagccgat gccctcggcg
     9121 aaggcgccct ccagctcggc gggggcgatg gcgacggact tgaggaagcc gagaacgtcg
     9181 gtgaaccaga gccggatgaa ccggatgtcg cgttcctcaa gcgtgcggag cacgaactcc
     9241 tgctggcggt ccaaggcatc ccctcgtgaa agacagtgcc ggtgacgctg cgccggtacg
     9301 tagagcgcgg gttacatacc agcatgccgc gcccgtgttt cgcacaggtt accgggggcg
     9361 gcggtgcgag cctcactgac aaagcgcccc tcccgactta gtgtgatcac gcagtcagtc
     9421 tcgggggagg cattggtggc catcgacctg ctcaatcaca aactggatcg ggcattcgac
     9481 cacatcgacg cgaacggcag gggcctcgtc gcccgcgagg acctgctggg gctgggcgcg
     9541 cgcatcctgg tcggcttcgg ggagtccccc acctcggtga ccggcaagag cctggtcgag
     9601 agcttcgacg gcatctggtc ggccctgacc ggcgttctgg ggcacgacgt ggacgcctcc
     9661 ctgacgcggg acgagttccg cgaggggatg gcggcggcct tcgtgacggg cggccactac
     9721 gacccggtgt tccgcccggc cacggtcgcg gtggccgagc tgtgcgacgg cgacggcgac
     9781 ggacggatcg gcaggcccga gttccgcacc atgctgacgg cgttcggcac cgcgtacgac
     9841 gacgtggacg cggcgttcga ccggctggac cgcaccggca ggggcacgct cggcgtcgag
     9901 gacctcgtcg tggccgcgcg ggagttctac ggcggcgacg acccgaacgc gtcgggcaac
     9961 tggctgttcg gcccgctgtg agaacggcag ggagcaccag atgtccacgg tgatgtccac
    10021 ggcggtgtcg acggccgtct ccacggcgat gtccacggcg cgcaccgctc tgcgctcggt
    10081 gctcaagccg ctgctgcccc tgccgcgcag gggcggcccg gccgcgctgg cgctgctgcc
    10141 cctgacggag cgggtgcccg ccctggcccg gccgtgccgg atgcaccctt cggtgtacgc
    10201 gatcgaggcc gccgtcatcg actgggccag gaggacgggg ctgcgcgccg atcccggggt
    10261 ggggtaccac cggatggccg gccgggcgtt cgccgagttc gacgccgccg cggcggcgct
    10321 gttcgcgcag tggctgacct ggctgttcca cctcgacgac gagctggacg agcggggcgg
    10381 cccgctggag gtgttccagg ggctgctggc gggcgcggcg tcgcacccgc tggaggcggc
    10441 cttcgcggac ctgtggcggg tgaccagcgc gcgcatgagc gaccgctggc gggcgaggtt
    10501 cgcggtcaac ctgcgccgtc agcacgaggc gtgccgggcc gaggccgaca accgcgcgtc
    10561 ggggcggatg ccggcgatcg aggagtaccc ggtgctgcgc aggggcacgg tggggccgta
    10621 cctgttcgac ctggtcgagc cgtgcctgcg ggtggaggtg ccggcctggg tgcacgagtg
    10681 cgagtcctgg caggagctgg tggacgcctg cagcgacgtg acggcctggt gcaacgacgt
    10741 ggcgtcgcgc ccgaaggagc gcgacgacgc gcacaacctg gtggtgctgg cggcgcgcca
    10801 gctcgggctg gccgagcgcg acgccgtcgc gtgggtgctc gaccgcatcg cccgccgctg
    10861 ccaggacatg cgcagggcgg ccaggcggct gccgctgcac gacctgccgc ccgcggcggc
    10921 gagggacgtc agcaaggtgg cgtgcgcgta cctggcggcg ccgcgcgccc acctcgactg
    10981 gctgctggag tcggcccgct acgcctgacc accgcgtccg gactacgcct gaccgccgcg
    11041 gccggccgct acgcccggcc ggcgagcatg tcgcccgtct gggtgcgccg gtagacgacc
    11101 tgcttgccga cccgcatgcc cacggtcagc ccgccggcgc tgagcacgcc gaggtgctgg
    11161 ctgaccgcgc cgggcgtgag cgccatgcgg gccgcgagcg ccgtggtggt gctgggctcc
    11221 gccagcgcga gcaggatccg cgcccggttc ctgccgatga gggcggcgag cgcgcccggc
    11281 gcgggcggcg gcccctcctc ccacagcgtc ccgacgccca gcacgggata gaccagcatc
    11341 gactggtacg gcgccgacat gacggccacg gacggccagt agaacgcggt cggcacgagc
    11401 acgagcccgt cgccgtgcag cccgcccgtg gcgcaccagg gccggtcgat gacgagctgc
    11461 tcaccggtcc agacgacggc cgggtcgagc ccggcgaaca gctcgcgggc gccttcgcgg
    11521 gcgagctggc gggacctgcg cagcacgtcg cgttcgagca gggcgtacac gcggggccag
    11581 aactcggcga agcactcctc ccagtaggcg agcagggcgt cggcgaccct ggcgacgccc
    11641 gccgcggggt cggcggcgaa gcggcgcagc acggcggggt cggtgccccg cacgatcgcg
    11701 gcctcggcgc gggcctgctc gggcggggtg ctcctgacgc ggtcgagctc ggcggcgaag
    11761 tcgggcagcg gggtgtcggg cggcggggtg aggaagtcgg ccaggtaccc ggtcggcggg
    11821 acgagcgcga gcagctcgtc caggtcgagc gcggccagac ggggccggac ccgtttgatc
    11881 cacggcaggt ggatcgactg cctggagggg tcctggagcg tgcgcaggct ggcgaccagc
    11941 tcccacatgg gcgagaaggc gaacctgatg cgggcgacgt cctcggcgtt gagccgccag
    12001 gtcacagtca tgcttttagt gtgcgctaaa acgttcgcgg cgcgggaacg cgggtcgcac
    12061 gctggttccc gtgtctaccg cgacctcctc cggcccctcc ccacgcgtcg tcaagaagcc
    12121 cgccttcgtg cgttccaagc tcggcgggct gccccgcccg ttctgggccc tgtggaccgg
    12181 cacgctcgtc aaccgcctgg gcagcgtcgt ggtgccgttc accggcgtct acctgacgca
    12241 ggagcgcggg ctgtccgtca ccgcggccgg cctcgtcatc ggcgtgtacg gcgtgggctc
    12301 gctggtgtcg cagatcctcg cgggcgtgct caccgaccgc gtcgggaggc gggccacgct
    12361 cgcgggcggc atgctggcca cggcggcggc gctgctcgcg ctcggctaca gcacgtccat
    12421 gccggcgatc ctggcctcca tggccgtgct cggcgtggtc accgacgtct accggccggc
    12481 gtccaacgcg atggtggccg acctggtcga cccggaggaa cggccacgcg cgtacggcct
    12541 gctgttctgg gggatcaaca tcggttacgc cctgggcatg acggcgggcg ggtggctggc
    12601 ggagcgcggg ttcacctggt tgttctggat cgacgccctc tccagcgtgc tcttcgccgt
    12661 gctggtgtgg cgggcggtgc ccgagaccag gccggccggg cgcgaggcgg cgggcggcgg
    12721 gttcggcgag gtgctgcgtg accggctgat ggtggcgttc acgctggtcg tgctgggtga
    12781 cgcgctcatg tacatgcaga ccgtctcgat gctgcccgtc gcgatcacga gggtggcggg
    12841 gctgacgccg gggcagctcg gcgtggcgat ggcgctgaac ggcgtgctca tcgtggtggt
    12901 gcagccgctg gtctccgact ggctggggcg cagggacccg gcgcgcacgt tcgccctggg
    12961 cctggccgtg atggggctcg gcttcgcgat gacggcgttc gtcacctcga cgctctggct
    13021 ggccgtgacg atcgtggtgt ggacggcggg cgagatcgtc acggcgggca tcgccggctc
    13081 gatcctggcc acgctggccc cgcctcacct acgggggcgc tactcggggc tgttcgggct
    13141 cgcctggtcg gcggcgatgg tgctggcgcc ggcgctcggc ggtccgctgc tggagatcgg
    13201 gcctgaggcg ctgtggttca cgatcggcgg ggtcggcctg gtctcggcgg cggggatgct
    13261 cgcgctgggc cccgccatcc gcagacggtg accgccccca ggcttttgag cgtcggcacg
    13321 gattgagcgg atgtcgatcg aacggcgaca tccccctcct accctccgag ggatgctgac
    13381 ctcacgaaaa atcgcggtat ggccgggcga cgcttacgct tacgcctgac atgctgacct
    13441 tcacggcgct cgggccgttt caggcctggt ccgacggcgc cccactcgac ctcggcggcc
    13501 agcgccagcg cgcggtgctc gcccgcctgc tcgtggcagg tggccgcgcc gtgcccgtcg
    13561 ccaccctgat cgacgagctg tggccgggca gcccgccgcc acaggcgctg tccacgatcc
    13621 aggggtacgt ctcacggctg cgccgcgcgc tcgaacctgg ccgcgcgccg cgcgaggagg
    13681 ccgaggtgct ggtctccgcg ccgcccggct acgccctgcg ggccgcgacc gaccaggtgg
    13741 acgcctggcg cttcgagtac ctggtgaagt ccgacggccc gcccgcgcag gtgtgggcgg
    13801 ccatggacga ggcgctggag ctgtggcgcg gtcccgcgct ggccgagttc tccgacctgg
    13861 cgtgggcggc caccgaggcg ggcaggctgg aggagctgcg gctcatcgcc gtggagcggc
    13921 gggccgccgc gggcctcgcg ctcggccggg ccacctcgct ggtggccgac ctggaggcgc
    13981 acgcctcggc gtacccgctg cgggaggagg gctggcggct gctcgccctc gccctgtacg
    14041 cgatggggcg ccagggcgac gcgctcgccg cgctgcgcag ggcccgcgcc gtctggcgcg
    14101 aggagctggg gctcgacctg tcggcggcgc tccagcggct ggagggcgac atgctggcgc
    14161 agcggctgga ggagcccgcc gcccccgcgg cgacggcgcc ggaggcgtcc ggcacgctgc
    14221 gcgtgctcgt ggccgacgac caggcgctgg tgcgcgccgg gctgcgggtg atgctggaga
    14281 gcgaggccgg cgtggcgctc gccggcgagg cggccgacgg cgaggaggcg atcgcgcggg
    14341 cccgcgagac gggcgccgac gtggtgctgc tcgacatcca gatgccgcac ctcgacggcc
    14401 tggccgcagc ccgcaggatc ctcgcgcagg agcgcccgcc gaaggtgatc atgatgacca
    14461 cgttcggcag cgacgacaac ctgtacgcgg cgctgcgggc cggtgtgagc gggttcctgc
    14521 tgaagacgtc gccgcccgag cagatcctcg ccgcgatcag ggccgccgcg gcgggcgacg
    14581 cgctcatcga cccggccgtc acgacgcgga tcatcgcggc gttcgccggg aggaccgatc
    14641 cggcctctcc cccggcgctg gccgggctgt ccgacgcgca gctcgacgtg ctgaagctgg
    14701 tggcgcgcgg cctgacgaac cggcagatcg gccagaccct cgccctgccc gagcacgagg
    14761 tggcggccgc ggtgtcggcg ctgctggagc atctcgggct gttcgaccgc gcgcagctcg
    14821 tcatggcggc gtacgagtcg ggtctcgtca ccccgggcgg catgtcacag aaatgaccga
    14881 cttttcgtcg cttggacgat aaaaaggttg gagtactagg ctggtcgcgt ggcccaactg
    14941 cgcatcgccc tggcacagac gaaccctgcc gtcggtgacc tgaccgccaa cgcggagcgg
    15001 ctggtcgcct ggacccgcga cgccgccgac cgcggcgcgc acctggccgt cttcaccgag
    15061 atgttcctca ccggctaccc cgtcgaggac ctcgtgctgc gcacctcctt cgtggaggcc
    15121 agtatcagaa cgctggagga gaccgcggcc aggctggagc gcgagggcct cggccacctg
    15181 cccgtcgtcg tcggctacgt cgaccgcgcc gggctcgccc cgcgcgtcgg ccagccgaag
    15241 ggcgcgccgc tcgacgccgc cgcgctgctc cacgaggggc gggtcgtcac ccgcacggcc
    15301 aagcaccacc tgcccaacta cggcgtcttc gacgagtacc gctacttcgt gcgcggcgac
    15361 agactgccga tcttccgcct gcacggcgtg gacgtcgcca tcgccgtctg cgaggacctg
    15421 tggcaggacg gcgggcccgt ctccgtcgtc ggccaggccg gcgccgggct gctcgtcgtg
    15481 cccaacgcct cgccgtacga gaaggagaag gacgacgtcc gcctggagct ggtcgcgcgc
    15541 agggcgcgcg aggccgggtg cgcgctcgcc tacgccaacc aggtcggcgg ccaggacgag
    15601 ctggtcttcg acggcgactc gatcgtggtc gacgcctccg gcgcgctcgt ggccagggcc
    15661 gggcagttcc gcgaggagct cctcgtcgtg gacctcgacc tgcccgtctc caccgccgcg
    15721 accggcatct tcccgtacga cgccggcgac ggcaccacca tcaccgtgga ccggttcgtg
    15781 gtctcgtccg agccggtgga gtcgtacgcg cccgagcccg gcgtcatcgc cgaacggctc
    15841 gacctgcccg ccgaggtcta ctcggcgctc gtgctgtccg tacgcgacta cgtggccaag
    15901 aacggcttcc agtcggtgat actcggcctg tcgggcggca tcgactcggc gctggtggcg
    15961 accatcgcct ccgacgcgat cggcccgtcg cgggtgcacg tcgcgctcat gccctcgcgc
    16021 tactcctccg accactccgt ctccgacgcc gacgagctgg tacgccgcca gggcatccac
    16081 gccagggtcg tgccgatcgc cgacatcgtc accgcgtacg agaaggagat cgacctgtct
    16141 ggcctggccg cggagaacct gcaggcgcgg gtgcgcggca tgatcctcat gggcctgtcg
    16201 aacgagcacg gccacctggt gctcaccacc ggcaacaaga gcgagctggc caccggctac
    16261 tccaccctgt acggcgactc ggcgggcggc ttcgccccca tcaaggacgt gccgaagacg
    16321 atggtgtggg agctggccag gtggcgcaac gcccacacct gccccgggtt cctgctcgac
    16381 gccgagcgcc cgatccccga gaactccatc gtcaaggagc ccagcgccga gctgcggccc
    16441 gaccagcgcg acaccgactc cctgccgccg tacgaggtgc tggaccgcct gctcgacgac
    16501 tacgtggaga aggacatggg ctcgcgggag ctcatcgcgg ccgggcacga cccggccctg
    16561 gtggcccgcg tgatccggct ggtggacctg gccgagtaca agcgcaggca gtacccgccc
    16621 ggcccgaaga tcacgccgaa gaacttcggc cgcgacaggc ggctgcccat caccaaccgg
    16681 tggcgcgaga gcgccggctg atcagcggtc agcggttcgc catggagccg caagggtttg
    16741 accccggcgc ggtaggcgcg cggttcggtg cggcgcgccc gggtaggcac ggcagtgctg
    16801 gtctggcacc agtgtggcca tcgctgcccg tcgtctcccc gccctgtggc ggaaacgcgg
    16861 ggccacgccg acctcgcatc tgaccaccgg agcgcgatgt gacgatcaaa gagcccggca
    16921 gcgagccgct gacctcgcca ctcgcccccg tgcatgcccg cacgggggcg gtcttgtcga
    16981 agtggctgtc gtcgaccgac cacaaggtca tcggctacat gtacctgatc gcgtcgttcg
    17041 tcttcttcct catcggcggg atcatggcgc tgatcatccg cgccgagctg gcccagcccg
    17101 gcctgcagct cgtcagcaac cagcagtaca accagctgtt cacgatgcac ggcacgatca
    17161 tgctgctgct gttcgcgacg ccgctgttcg ccgggttcgc caacgtcatc atgccgctgc
    17221 agatcggctc gcccgacgtc gcgttccccc ggctcaacgc cgccagcctc tggctgttcg
    17281 tcttcggcgg catcatcgcc gtcagcgggt tcttcacctc gggcggcgcc gccgacttcg
    17341 gctggttcgc gtacgcgccg ctgaacagca agacctacag ccacctcatc gggtgcgacc
    17401 tgtggatcat ggggctggtg ctgtcggggc tgggcaccat cctcggctcg gtgaacttct
    17461 tcacgacgat catctgcatg cgcgcgcccg gcatgacgat gttccgcctg cccatcttct
    17521 gctggaacac gctgctgacc agcatgatcg tgctgatggc gttccccgtg ctcgccgccg
    17581 cgctgctcgt gctggagctg gaccgcaggt tcggcaccgt ggtcttcgag gcgcccaacg
    17641 gcgggcccat cctgtggcag cacctgttct ggttcttcgg ccaccccgag gtctacatca
    17701 tcgcgctgcc gttcttcggc atcgtcaccg aggtgctgcc cgtcttcagc cgcaagccga
    17761 tcttcggcta catcgggctg gtggccgcga ccatcgccat cgccggcctg tcgatgacgg
    17821 tgtgggccca ccacatgttc ggcaccggcc aggtgctgct gccgttcttc tcgttcatga
    17881 cgttcctcat cgccgtcccg accggtgtga agttcttcaa ctggatcggc accatgtggc
    17941 gcgggcacct caccttcgag ccgccgatgc tgttctccgt cggcttcctc gcgacgttcc
    18001 tgctcggcgg gctcaccggg gtgatcctgg cctcgccgcc gctcgacttc cacatcaccg
    18061 acacctactt cgtggtggcc cacttccact acgtggtctt cggcaccgtc gtgttcgcca
    18121 tgttctccgg cttctacttc tggtggccca agttcaccgg caagatgctg gacaagaccc
    18181 tcggcacctg gcacttctgg ctgctgttca tcggcttcca cacgacgttc ctcatccagc
    18241 actggatcgg cgcgatcggc atgccgcgcc gctacgccga ctacagcccc gccgacggct
    18301 tcaccgacct caacatcatc tcgtcgatcg gcgcgctcat cctcgggatc tcgacgctgc
    18361 cgttcctcta caacgtctgg cacaccgcgc ggtacgcgcc gcgcgtcacc gtggacgacc
    18421 cgtgggggta cggcaactcg ctggagtggg cgacgtcgtg cccgccgccc cgtcacaact
    18481 tcacgggcat cccgccgatc cgttccgagc ggcccgcgtt cgacctgcac tacccccact
    18541 cgcagtggcg ggaggtcgag agcccctgac ggcgacgccg agcggggccc ggccgggtgg
    18601 cggccgggcc ccgctctcgc tctgcacgcc gcctgcctgc tacgacgggg ccggggacgc
    18661 gggccgggag gacggcgggg acggctggga gggcgtggcc accggggccg tcccggccgg
    18721 cgacgggaca ggcgggcccg ccggcgacgg cgtgaggctc gcgtagatgc ccgtcctccg
    18781 catgaccggc agcagcaggc ggaggtcacc cgcgttcctg aacgagaacg tcatcgcgac
    18841 cgggccaccc ttgacgccgc tgaccgaggc gatcggccgg ttgcccgtgc ccaccggctg
    18901 gttcggcggg agctcgatcg ggccgggcag ctgcacctgg ccgccgccct ccacggtgat
    18961 gcgttccagg gtgtccggct tcgccgccga gttgatcaac accgcgaaca gcgcgtcctg
    19021 gccgccgccc tggcccgtcg gctgcccgga gggcgacgcc gggccggagg cggaaggcgg
    19081 ggaggcgggc gggctggagg gtgaggccgc gggcggggcg gcggacgtgg tcgcgccgct
    19141 cagcaggaag gcgttgcgca cgtgcagcac gcccttgagg tcgtagttcg tgccctcgtt
    19201 cggctggtag tgccgaaggc tggcgtcggt ggactggttc gcgcaggcgg ccgtcacgac
    19261 agccgtggcg acgagcgcgg cgaccaaggg acgcatctac gcagcctcct cccgttacgg
    19321 atcgtcggct ccccgctacc cgggaacacc gccccgaacc gtcacgccac ccccaccgcg
    19381 cacccccggg ccctggcgac cggtgcggcg gttacggcag gcggcacagg aggcgcgagc
    19441 aggccatcgg ccccggcggc cggtgtggcc gtacgagggg cggcgcctag ggggtgaggc
    19501 cgtgcagggc cgcgtcgacg acccgctcgg ccaggtcggg cgcgaggtcg cccgccgggc
    19561 cccacttcgt gtaccagagc atggtccccg acaggaccgc cgtgcccaac tcgacgtcca
    19621 ggtccgggcg cagctccccc gaggcgatgc cgcgctcgat caccgcgcgc agcgcctccc
    19681 tgcgcggttc gatggcgcgc ttgaagaagc gctccatcag ccgcgggtag cgatcggact
    19741 cgctcatggc gatgttcatg acacagcgcg aacgggcctg ccccgactcc ctgcgcatcg
    19801 cgtcgagcag ggcgatcaag tcctcgcgta ccgacgtccc ggacaagggc ggcagcggcg
    19861 acttgagcgt ggacagcgcg tcgacgacga ggtcctcctt gttggaccag cgccggtaga
    19921 tcgtcgtctt gcccacgccc gccctcgacg cgatcgcctc gatggacagc tcggagacgc
    19981 ccatgccctc gcctatcagg tcgagggtcg cctcgatgat cgccctctcg gccttctcgc
    20041 tgcgcgggcg gcccgcagga cgggtcgtcc ctgattcctg gatcgtcatc gcggcctcac
    20101 actaccgctg gttccttctg ttccgcctgc tggacaggct tcgccttgcc gggcatccac
    20161 ctggccacga ccgccacgcc caccagcgcg atgaccgcgg agaccagggc cgtgacgtgc
    20221 atgccgtcga cgaaggcgga gaaggcgggc ttgatcagcg cctggccgcg ctcgccgagc
    20281 gcctgcgcca ggcccaccgt cgccatgatc gactcaccgc ccttgtgctg gacgtccgcc
    20341 ggcaggccgg cgagcgcggg cgcgatctca ccccggtagc tggacgacag caccgagccg
    20401 agcactgcca cgccgagcgc gcccgccacc tggcggacgg tgttgctcat ggccgagcct
    20461 acccccgcct tctccctggg cagggactcc atgatggcgg tggtcgcggg cggcatgatg
    20521 ttggccatcg cggcgccctg gacgaagaag acgacctcga tgagcaccac cggggtgtcg
    20581 gcgtccagga aggcgtagct ggcgagggcc gccgtcacca cgagcatgct gaccgtcgcc
    20641 gacagcttcg cgccgaaccg ctggttcacc cgctggctca gcggggcgaa gatgagctgc
    20701 gccgccgcga acgggatcat cagcgcgccc gcctccaccg gcgtgaaccc gagcacgatc
    20761 tgcaggtaga aggtcaggaa gaacatcccg cccatcatcg cgaagaagac gatgcccatc
    20821 atgccgatgg ccgagctgaa cctgatgttg gtgaagttgc ggacgtcgaa gaccgggttg
    20881 tcgatgcggc gctcccacca cacgaacgcg cccagcaccg ccaggccgat cagcgtcggc
    20941 accagcacct gcgcgttggc cacggtcgcc agctcgccgc cgcggatgat gccgtacacg
    21001 agggccac
//
