LOCUS       NZ_BBZG01000001        21068 bp    DNA     linear   CON 12-AUG-2024
DEFINITION  Nonomuraea pusilla strain NBRC 110462, whole genome shotgun
            sequence.
ACCESSION   NZ_BBZG01000001
VERSION     NZ_BBZG01000001.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nonomuraea pusilla
  ORGANISM  Nonomuraea pusilla
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft Genome Sequence of Nonomuraea sp. TP-A0861, a Producer of
            Myxochelin A
  JOURNAL   Genome Announc 3 (6), e01430-15 (2015)
   PUBMED   26659677
  REMARK    DOI:10.1128/genomeA.01430-15 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746196)
  AUTHORS   Hosoyama,A., Kimura,A., Ichikawa,N. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2015) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, Biological
            Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.8
            Genome Coverage                   :: 107x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417755.1-RS_2024_08_12
            Annotation Date                   :: 08/12/2024 01:46:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,307
            CDSs (total)                      :: 8,242
            Genes (coding)                    :: 8,159
            CDSs (with protein)               :: 8,159
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 21 of 83
            Pseudo Genes (incomplete)         :: 72 of 83
            Pseudo Genes (internal stop)      :: 5 of 83
            Pseudo Genes (multiple problems)  :: 15 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:25:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3049420
            Orig. end                         :: 3070488
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBZG01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21068
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="12"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21068
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21068
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11068](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11068
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            149..841
                     /locus_tag="ASC57_RS13440"
     CDS             149..841
                     /EC_number="5.1.3.9"
                     /GO_function="GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /GO_process="GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604774.1"
                     /locus_tag="ASC57_RS13440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="WP_055503143.1"
                     /transl_table=11
                     /translation="MSVADVLGLLRGGLVVSCQAPAGHPLRDPGVIAALAECAVLGGAA
                     GLRINSPADIRAVRRRTALPVIGLHKVRHGHRDVITPSLELAAGLAEAGADVIAIDLTP
                     ESPAHGPDLVAAVRDELGRPVMADVSTLEEGLAAWGAGADVVGTTLSGYTARQLPPPDD
                     PDIELVAALAARGVRVVAEGRYRTEEQVRRAFDAGAWAVVVGSAITDPVAITARLRAAT
                     PRAVDEPR"
     gene            838..1776
                     /locus_tag="ASC57_RS13445"
     CDS             838..1776
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1108: ROK family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /locus_tag="ASC57_RS13445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ROK family protein"
                     /protein_id="WP_055503144.1"
                     /transl_table=11
                     /translation="MTPLALAVDIGGTKIAAALVDGEGRLAERRRVPTPASEGGEAIMA
                     AVAAVSAPLLAAGGSRVVACGIGTAGVVDGRGRVASATDLLTGWAGTDVKGVAERTLGL
                     PAVVMNDCHAAGAGESRLGAARDAETALVVAVGTGIGGALCRSGAPVTGRTGTAGSIGH
                     VPAPPPPALPHGPAPAPRRCSCGATDHVEAYASGPAVERAYREAAGRALPLAEIGRRAA
                     AGDADAERVIGDAATLLGRVLAGAANLLDPDVIVIAGGVSLLGPPLLAPLASAFRAEAL
                     PGPRDIPVRAAALGGDAVLVGAGTHALARGR"
     gene            1825..3303
                     /locus_tag="ASC57_RS13450"
     CDS             1825..3303
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ASC57_RS13450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_055503145.1"
                     /transl_table=11
                     /translation="MKSRPGPSRLRPSLAVAATALLTAATLASGAAPAAAQDAPSQSVL
                     RHRALHGASIEIDPTFPYYKDRSPDSIADEIARNGYTAVHYFVVDENRVDGELVAALRE
                     RGIAVWALTLAFGSYTVEGYPAEWPSWEQKLLSASQPGFHSFSPWSADYLAFKKKSLGD
                     LIKKYPFDGIELAESFLPDWNGFTSGFYGDLGPLAEAAFKAEYGLDMPDFTDPSSPRYY
                     KTDTARYAKWVDFRVAGVNRFLGDLVRGEGGLRSARPGVLVGSWSVAVDAGPDSVRLER
                     ELQAVDAPAMIAAVRPDIHFIQTNWPDWLKPDLAPDYIKGYQPYVDEIRRSNPALPLGV
                     QNDIGSTTGTARDRAWIGAFSDTASSMGFSTWTGYEFSLAKAMYTEAPEVRAIRCDRAA
                     GTVEVSFQKRIDAASAARPDSFAVRTPAGRSHGVLGVEVDGNIAKLRLDGLPRGRFELA
                     VRNVSDTPSLWVFNKTQQPNTVEPGYTAEGRCTG"
     gene            complement(3357..3548)
                     /locus_tag="ASC57_RS13455"
     CDS             complement(3357..3548)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1265: ferredoxin"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536827.1"
                     /locus_tag="ASC57_RS13455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ferredoxin"
                     /protein_id="WP_055503146.1"
                     /transl_table=11
                     /translation="MRINADTSVCIGAGMCALTVPKVFDQSEEDGTVVLLQKEPPAELD
                     EAVRRAVNLCPSGAISIA"
     gene            complement(3555..4763)
                     /locus_tag="ASC57_RS13460"
     CDS             complement(3555..4763)
                     /EC_number="1.14.-.-"
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541682.1"
                     /locus_tag="ASC57_RS13460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_055503147.1"
                     /sec_met_domain="p450 (E-value: 7e-17, bitscore: 58.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDLTVPALPTVRERPLDPPDEVARLRQQGGVHRMTYADGHVGWL
                     VTGYSAARAVLADSRFSNRPENMHPPIPGRFAAGKELRERGFQTPPGFFLRTDPPEHTR
                     YRKLLTGQFTVRRMKLLEPRIEEITASALDALEAAGPPADLVQDFALPIPSLVICELLG
                     VPYEDRARFQDDSKILVNLETGAEEAIAAIGRVFAYLHDLIGRKREHPADDLISGLIEG
                     GELKDDELAGVSFLLLVAGHETTANMLALGTYTLLTNPGQLAVMRGDPGAVENAVEELL
                     RYLTIVHLGPIRTAIEDVEIDGHLVRAGESVTLHLPAANRDPDRFPGGDTLDLTRPAGG
                     HLSFGHGIHQCLGQQLARVEMRIAYPALLRRLPGLRLAVPPEEVPMRSDMSIYGVHRLP
                     VTW"
     gene            4841..5464
                     /locus_tag="ASC57_RS13465"
     CDS             4841..5464
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892714.1"
                     /locus_tag="ASC57_RS13465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_236714693.1"
                     /transl_table=11
                     /translation="MQYSSAMVGLRERKKQRTRRALVDAALRLFADKGYEETTLAEIAA
                     AADVSTRTFFSYFASKEDVLFYDAEERMERLLALVASRGPHESPADLLLRLIDEIVYRS
                     GEEEDLTLEETRLRMRLIMSVPPLQARALFLLFDTQLRLAEALCEAYPGRLGPLDAAAA
                     VGALTGAVKLAAMAAISRGASLQEISDTARRAAALAASGLRSLG"
     gene            complement(5478..6347)
                     /locus_tag="ASC57_RS13470"
     CDS             complement(5478..6347)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887923.1"
                     /locus_tag="ASC57_RS13470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_055503148.1"
                     /transl_table=11
                     /translation="MILVTGVSGALGGSILRGLRAHGGLEVAGGSRAGDGGAGGVRLVD
                     FDRPRTLPGAFAGVDVLVLVSAGYAEDDVVLARHGAVVRAAAEAGVRHVVYTSLAGSGD
                     PLSIALAHRWTEARLAEAPFEVTVLRNGLYAELLADLALAGTVGDVLAAPLADGAMPVV
                     ARDDLADVAVRVAAETHADLAAGRPRRHAGRTYELEGETALGGADLAALLTEALGRPVG
                     YRPVPLGEARAALGASGLEPYQVGHTLSLFSAAGAGLMRSRGGDLRAFLDVPRPAARVV
                     AARLASAR"
     gene            6436..6855
                     /locus_tag="ASC57_RS13475"
     CDS             6436..6855
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581525.1"
                     /locus_tag="ASC57_RS13475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_055503149.1"
                     /transl_table=11
                     /translation="MSVGHTAITAQLSGQAAAQAAGPCGDDDCGIRDVLDRLGDRWTVL
                     VVVELAKGVRRFRELQRAIPGISQRMLTLTTRRLCRDGLVERTAYPTIPPQVEYRLTPM
                     GHSLAEAVASLADWSRGHKDAIAASRARWDAERTG"
     gene            complement(6886..7650)
                     /locus_tag="ASC57_RS13480"
     CDS             complement(6886..7650)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889495.1"
                     /locus_tag="ASC57_RS13480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="L,D-transpeptidase family protein"
                     /protein_id="WP_236714694.1"
                     /transl_table=11
                     /translation="MTLSGAGALAGPAAAATVTGSATPARSATVQQAEYARFAAAPVKT
                     LKLGARGEAVVWMQNRLAGLGYLPGKADGRYGGTTLAAVWAFQKVNGIRPTSTITRATW
                     AALERPKAPQVLVKNGRPTRAEVNLTKQVMVLYVDGRPKLISHISSGSGVPYCETATWQ
                     GRKQRFCGSASTPTGDFRTTWRRSGWHTSYLGRLYNPIFFNGGIAFHGALSVPLYPASH
                     GCVRLPMNVAEVLPGLLGTGVPVHVRGRFHRA"
     misc_feature    7732..7746
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 13.98"
                     /tool="antismash"
     misc_feature    7782..7800
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 16.87"
                     /tool="antismash"
     gene            7802..8278
                     /locus_tag="ASC57_RS13485"
     CDS             7802..8278
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131598.1"
                     /locus_tag="ASC57_RS13485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YbaK/EbsC family protein"
                     /protein_id="WP_055503151.1"
                     /transl_table=11
                     /translation="MSEKLPGNAVKVDAALRALGATGEIVVLPEKAPTAATAAAQLGCE
                     VGAIANSLIFDADGAPLLVLTSGAHRVDVGLIARTAGVTEVRRATPEFVRAATGQPIGG
                     VAPVGHPAPVRTLVDNWLSKHEVVWAAGGHPLTVFPTTFDELVRITGGTPVDVE"
     misc_feature    8344..8358
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.14"
                     /tool="antismash"
     misc_feature    8411..8427
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.06"
                     /tool="antismash"
     misc_feature    complement(8431..8451)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.68"
                     /tool="antismash"
     gene            8457..8864
                     /locus_tag="ASC57_RS13490"
     CDS             8457..8864
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /locus_tag="ASC57_RS13490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="N-acetyltransferase"
                     /protein_id="WP_236714695.1"
                     /transl_table=11
                     /translation="MRDSPGEGGAPRPPIVVGFAYGFRGAPGQWWHDVVYRALEERAGE
                     EAAHAWLGFAFELAEIHVHPDHQGRGIGRAMIRTLCAGRPERSAVLSTHDRPTAARHLY
                     AAMGFTDLLARFVFPGGYEEYAIMGRPLPLD"
     gene            complement(9014..9931)
                     /gene="metF"
                     /locus_tag="ASC57_RS13495"
     CDS             complement(9014..9931)
                     /EC_number="1.5.1.20"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA]"
                     /GO_process="GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="metF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131596.1"
                     /locus_tag="ASC57_RS13495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="WP_055503153.1"
                     /transl_table=11
                     /translation="MALGRPSVLPDRVPTIRELLASGGRSFSFEFFPPKTDEGERQLWR
                     AIRELESLRPTFVSVTYGAGGSTRDRTVDIVERLAHDTTLTPVAHFTAVNHSVRELRHL
                     VGRFADAGVRNILAVRGDPPGDVTGEWVQHPEGVLYAEELVRLIRQSGDFCVGVAAFPY
                     KHPRSPSIESDTEYFVRKCRAGADFAITQMFFRADDYLRLRDRVEAQGCDTPIIPCIMP
                     VTQMSTIARSEQLSGAPFPAEVAARFEAVADDPAAVRALGIEHGAELCQRLLDEGAPGI
                     HFITFNRSSASREVFQRLDVRQPA"
     gene            10001..11068
                     /locus_tag="ASC57_RS13500"
     CDS             10001..11068
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889490.1"
                     /locus_tag="ASC57_RS13500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_055503154.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.3e-45, bitscore:
                     153.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNDAASMGTLRAHVEQALREFVERQLPASGDPDLEPLRTAAHEL
                     LAGGKRLRPAFCYWGWRGAGGSDVPEIFTAAASLELLQASALVHDDVMDKSDLRRGMPS
                     AHRRFQTLHEKAGWHGSAEQFGEGAAILLGNLLLIWSGEMWRTCGLPARSLAAAQPVHD
                     HMRTELMCGQYLDLLEQAHGENTFDSALRVALYKSGKYSVEQPLRLGLALAAGELLPWM
                     DELCAGYGRRVGIAFQLRDDILGVFGDPAQTGKPAGDDLREGKRTMLIARTLAAASPAA
                     AATVRRLLGDPGLDADGVAALREIIDGTGALRACEDLIGEYLGEGLAALEGAPITQEAR
                     TALTELAIAATSRRT"
     gene            complement(11203..11850)
                     /gene="thiE"
                     /locus_tag="ASC57_RS13505"
     CDS             complement(11203..11850)
                     /EC_number="2.5.1.3"
                     /GO_function="GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1280: thiamine-phosphate pyrophosphorylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591126.1"
                     /locus_tag="ASC57_RS13505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thiamine phosphate synthase"
                     /protein_id="WP_055503155.1"
                     /transl_table=11
                     /translation="MPKPSLSEALAGARLYLCTDGRRERGDLAEFLDVVLAAGVDIVQL
                     REKGLEAREELALLEVFRDACDRHGRLLAVNDRADIAYAARPDVLHLGQDDLPVTVARE
                     ILGDDLLIGRSTHSMAEASAAAVEEGVDYFCCGPIWPTPTKPGRHAPGPALLRHAASLG
                     ADRPWFGIGGIDLTTLDEVMAYGVTRVVVVRAITEADDPGAAAAEFAKRLAR"
     gene            complement(11828..12202)
                     /locus_tag="ASC57_RS13510"
     CDS             complement(11828..12202)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541673.1"
                     /locus_tag="ASC57_RS13510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="WP_055503156.1"
                     /transl_table=11
                     /translation="MTLSVAQIDRETEQLVDEWLSLPEVAARLELPLGRVKQLLKDHKL
                     LGVRRGSGGPHVPAVFLDGKDVRKGLPGTLTVLQDAGYDDVEALRWLFTPDDSLPGTPM
                     DAIKAGRHTEVKRRAQALAF"
     gene            complement(12233..13402)
                     /locus_tag="ASC57_RS13515"
     CDS             complement(12233..13402)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1175: pyridine nucleotide-disulfide oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541672.1"
                     /locus_tag="ASC57_RS13515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_055504048.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2.3e-50, bitscore:
                     169.2, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVKHVVVVGAGLAGVRSVEALRARGFAGRITLIGQERHRPYDRP
                     PLSKAVLLGDADSSFVDTDLSALEVDFWAGVSAKALRDGAVETTEGDLGYDGLVIATGA
                     EPITLPGDGPQHVLRTLDDAHELRALLVPGARVAVIGAGWIGAEVATAARRAGCAVTVI
                     EAGEAPLAHALGPELGARTTGWYEGVDLRLGTMVHEVREGGVELVGGEFVDAHVVVTGI
                     GVRPAVEWLASAEIDLHNGVVTDEHLRTSLPNVVAAGDCAAWWSRRFQSRLRVEHWDTA
                     LHAPEVAAATLLGEDAVYDPVPYFWSEQFGHMLQYAGHHPAGERLVYRGEPEGKWSACW
                     LTAGDTLAAVLAVDRPRDLVQGRRIIEAGHRLDAERLVDPDVALRDCVV"
     regulatory      13456..13573
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    complement(13530..13543)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     gene            13563..14759
                     /gene="thiO"
                     /locus_tag="ASC57_RS13520"
     CDS             13563..14759
                     /EC_number="1.4.3.19"
                     /GO_function="GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene="thiO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541671.1"
                     /locus_tag="ASC57_RS13520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycine oxidase ThiO"
                     /protein_id="WP_063792005.1"
                     /sec_met_domain="DAO (E-value: 1.6e-70, bitscore: 236.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVTDVMDVVVGGGVVGLAVAWRLAREGRPVTVVDPAPASGASHA
                     AAGMLAPVSEVTYTEAPLLRLGAASLRRWPSFAAELAADAGLGDPARALDLRADGTLDV
                     AFGADDLAALDELAAFMEKLELPVERLTGRECRRLEPMLAPSVRGGLLAPGDAWVDPRR
                     VTAALLAALDRLGVPLVRARVAGLLRDGDRVTGVRLADGPGGTGLQESGPREIRAANVV
                     LATGAWSGELLDLPVRPVKGQIMRLRSPQPLLGRCVRGMVHGAPVYLVPRGDGELVLGA
                     TQEELGFDTRVTAGGLYELLRDARELVPGVTELEVADMVAGLRPGTPDNLPLIGPAGVP
                     GLVLATGHHRGGVLLTPLTTDLVLDALTAAGGGARETETGETGELAELCSPRRFQETS"
     gene            14756..14956
                     /gene="thiS"
                     /locus_tag="ASC57_RS13525"
     CDS             14756..14956
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541670.1"
                     /locus_tag="ASC57_RS13525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sulfur carrier protein ThiS"
                     /protein_id="WP_055503158.1"
                     /transl_table=11
                     /translation="MIVMINGTAHEVPDGTTVAQAVRTLTTATAGVAVAVNDEVVTRGS
                     WETTALSDSDRVEVLTAVQGG"
     gene            14949..15704
                     /locus_tag="ASC57_RS13530"
     CDS             14949..15704
                     /EC_number="2.8.1.10"
                     /GO_function="GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541669.1"
                     /locus_tag="ASC57_RS13530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thiazole synthase"
                     /protein_id="WP_055503159.1"
                     /transl_table=11
                     /translation="MDELTIAGESFSSRLIMGTGGAPSLDVLDQALEASGTELTTVAMR
                     RLDPAARGSVLDVLRKRDIKVLPNTAGCFTAGEALLTARLAREALGTNWVKLEVISDER
                     TLLPDPIETFDAAERLVAEGFVVLPYIGDDPALARRLEQAGCAAVMPLGAPIGSGLGIR
                     NPHNIELIVEAASVPVILDAGIGTASDAALAMELGCDAVLLATAVTRAQRPDTMAAAMR
                     AAVEAGRLARLAGRIPRRRYAEASSPMTP"
     gene            15846..17819
                     /gene="pknB"
                     /locus_tag="ASC57_RS13535"
     CDS             15846..17819
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="pknB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541668.1"
                     /locus_tag="ASC57_RS13535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="WP_055503160.1"
                     /sec_met_domain="Pkinase (E-value: 6.6e-51, bitscore:
                     170.8, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTTTADPLVGRLLDGRYRIESRLARGGMATVYLALDVRLDRTVA
                     LKVMHRSLADDPAFVRRFIGEAKSVASLSHPNVVHVFDQGTDGEIVYLSMEYVPGRTLR
                     DILRERGRMPAREALEIMIPVLAALGAAHSAGMVHRDVKPENVLMTDDGRVKVVDFGLA
                     KAIEATNQTRTGVLIGTIGYMAPEQVMTGAADVRSDVYAAGIMLFELVTGQQPYDGETP
                     MSVAYRHVHDTVPLPSSIVPDVPPPIDALVAHATAREPHDRPADASAMLVEAVETHRAL
                     PRLTTPPASHSQPHARPASGAHSVPQPTQALPQAAHSQPHAAHSAHSAPSAPMAAPSHT
                     MIQPRVEAPERRRGFRPSWFLVALAAVMAVAVGVTAWFFSRPTLVIVPDLVGKDMALAE
                     QSAARLGFKVRKAEGRYDEKVPAGLVLSMEPVAKTEVEKGTQITLVPSLGAKRIVVPNV
                     AGMSQNDARLAIAAAGLTVGAVKRLPDEQVERDKVIRTSPQVGEKVKEGAKVGIYVSAG
                     LVMPDVSNMPKDEAAAYLGGRGFQVQVNEVDDDAEPCTVIAQSPKARSEVDKGAVVMVT
                     VARCQTDFWQWFNGDNEARDDQDVRLVPAVIGKNVKDAKADLEALGFHVRVNRLSNGGV
                     VQFQRPLPNSERPPGTTVYLWQ"
     gene            complement(17851..18810)
                     /gene="alc"
                     /locus_tag="ASC57_RS13540"
     CDS             complement(17851..18810)
                     /EC_number="3.5.3.4"
                     /GO_function="GO:0004037 - allantoicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="alc"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633155.1"
                     /locus_tag="ASC57_RS13540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="allantoicase"
                     /protein_id="WP_055503161.1"
                     /transl_table=11
                     /translation="MPDFTSLPDLALRTFGGAVVAASDESFAEKENLIRPGRPGFTPET
                     FGGKGQVYDGWETRRRRSPGHDWALVRLGLPGVVRGVAIDTAWFTGNHPPHAWVEATEA
                     DGHPSPSELAAARWTTLVPRSPLTGDAEHLFPVADEARYTHVRLAIDPDGGVARLRVHG
                     EPRADLSVYDGLGLDLAALANGGLVTACSDAFYSAPHNVIAPGLARHQAEGWETARRRE
                     PGNEWLVIRLAAPGRVRLAEIDTANLVHNAPAEVALTGLPPGPGGDDREIELLPRTPLR
                     PDTLHRFTLRHTEPVASVRVDIHPDGGLARVRLFGHPA"
     gene            complement(18811..20121)
                     /gene="allB"
                     /locus_tag="ASC57_RS13545"
     CDS             complement(18811..20121)
                     /EC_number="3.5.2.5"
                     /GO_function="GO:0004038 - allantoinase activity [Evidence
                     IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0050897 - cobalt ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="allB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541666.1"
                     /locus_tag="ASC57_RS13545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="allantoinase AllB"
                     /protein_id="WP_055503162.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.8e-25, bitscore:
                     87.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAYELVVRSRRVVTPEGERAAAVAVRGGRVAAVEPYGAPLAAPE
                     DVDLRDLALLPGLVDTHVHVNDPGRAHWEGFETATRAAAAGGVTTIVDMPLNALPPTVS
                     PDALDAKLRAAEGRCHVDVAFWGGAVPGNVEELRPLHDRGVRGFKCFMSPSGVDEFPPL
                     SPAELRATLTEAAGFGGLVVVHAEDPALLREPGGPSYRQFLESRPDAAERGAVESLAEE
                     AARTGARVHVLHVSSAACLDVLEAARARGVRITAETCPHYLTLDAEQVPEGETAYKCCP
                     PIRSRANRDLLWDGLARGVLDCVVSDHSPSTPDLKVPDFAAAWGGVSSLQLGLPAVWTE
                     ASRRGFGLPDVVRWMAERPAELAGLPGKGAIAPGRDADLVAFDPDAAVRVDAARLHHRN
                     PVTPYHGRTLRGAVLTTWLRGRPVDGLPHGRFLLGAE"
ORIGIN
        1 cgatcggtct gtacgccttc ttcggcgagc acgacatcgc ctggggcccg ctgatggccg
       61 cctgcgtcat gttcacgatg cccgcgctgc tcatcttcat cttcggccag cggtacttcg
      121 tgggcggcat ctcggcgggg gcgttcaagt gagcgtcgcg gacgtgctcg gcctgctgcg
      181 cggcgggctc gtcgtctcct gccaggcgcc cgcggggcac ccgctgcggg accccggcgt
      241 catcgccgcg ctggccgagt gcgcggtgct gggcggggcc gcgggcctgc ggatcaactc
      301 ccccgccgac atccgcgcgg tacggcggcg caccgcgctc ccggtgatcg gcctgcacaa
      361 ggtcaggcac ggccaccgcg acgtcatcac cccgtcgctg gagctggccg ccggcctggc
      421 cgaggcgggc gccgacgtca tcgcgatcga cctgacgccc gagtccccgg cgcacggccc
      481 tgacctggtc gcggccgtgc gggacgagct gggccgcccg gtgatggcgg acgtctccac
      541 cctggaggag ggcctggcgg cctggggcgc gggcgccgac gtcgtgggca ccacgctgtc
      601 cgggtacacc gcccgccagc tgccgccccc cgacgatccc gacatcgagc tcgtcgcggc
      661 gctggccgcc cggggcgtca gggtcgtcgc cgagggccgc taccgcaccg aggagcaggt
      721 acggcgggcg ttcgacgcgg gcgcctgggc ggtcgtggtc ggcagcgcca tcaccgaccc
      781 cgtcgccatc acggcgaggc tccgcgccgc gacccctcgg gccgtggacg agccgcgatg
      841 acgccgctcg cgctggcggt cgacatcggc ggcaccaaga tcgccgccgc gctcgtggac
      901 ggcgaggggc gcctggccga gcggcgcagg gtgcccacgc ccgcctccga gggcggtgag
      961 gcgatcatgg cggcggtggc ggccgtgtcg gccccgctgc tcgccgccgg cggctcccgg
     1021 gtggtggcct gcgggatcgg cacggcgggc gtcgtggacg gccgcggccg cgtcgccagc
     1081 gccaccgacc tgctgacggg ctgggcgggc accgacgtca agggcgtcgc agagcggacc
     1141 ctcgggctgc cggccgtcgt gatgaacgac tgccacgcgg cgggcgccgg cgagtcgcgg
     1201 ctgggcgccg cccgcgacgc cgagacggcc ctggtcgtcg ccgtcggcac cggcatcggc
     1261 ggcgcgctgt gccgctccgg cgcgccggtc accgggcgta ccggcacggc gggcagcatc
     1321 ggccacgtcc cggctccgcc gccgcccgcc ctcccccacg gccccgcgcc ggcgccgcgc
     1381 aggtgctcct gcggcgcgac ggaccacgtc gaggcgtacg cgtcgggccc ggccgtcgag
     1441 cgcgcctacc gcgaggcggc cgggcgcgcg ctccccctcg ccgagatcgg gcggcgcgcg
     1501 gccgccggcg acgccgacgc ggagcgcgtg atcggggacg cggcgaccct gctgggacgc
     1561 gtcctggccg gagccgcgaa cctgctcgac cccgacgtga tcgtgatcgc cggcggggtc
     1621 tccctgctcg gcccgcccct gctggcgccg ctcgcctccg ccttccgcgc cgaggcgctc
     1681 cccggccccc gcgacatccc cgtgcgcgcg gcggcgctgg gcggcgacgc cgtcctcgtc
     1741 ggcgcgggca cccacgccct cgcccgcggg cgatgacgca tcggaccctc ccctcccccc
     1801 gaccccccga ccgattggac gaccatgaag tcacgccccg gcccctcccg cctgcgcccc
     1861 tccctcgccg tcgccgcgac ggcgctgctc accgccgcga cgctggcgtc gggagccgcg
     1921 cccgccgcgg cgcaggacgc gccgtcccag agcgtgctgc gccaccgggc gctgcacggc
     1981 gcgtcgatcg agatcgaccc gaccttcccc tactacaagg accgctcccc ggacagcatc
     2041 gccgacgaga tcgcccgcaa cgggtacacg gccgtgcact acttcgtcgt cgacgagaac
     2101 cgggtcgacg gcgaactggt cgcggcgctg cgcgaacgcg gcatcgcggt ctgggcgctg
     2161 acgctggcct tcggctcgta cacggtcgag ggctacccgg cggagtggcc gtcgtgggag
     2221 cagaagctgc tcagcgcctc gcagcccggc ttccactcgt tctcgccctg gagcgccgac
     2281 tacctggcct tcaagaagaa gtcgctgggc gacctgatca agaagtaccc gttcgacggc
     2341 atcgagctgg ccgagtcgtt cctccccgac tggaacggct tcacctccgg cttctacggc
     2401 gacctggggc cgctcgccga ggcggcgttc aaggccgagt acggcctgga catgccggac
     2461 ttcaccgacc cctcctcgcc gaggtactac aagaccgaca ccgcccgcta cgccaagtgg
     2521 gtcgacttcc gcgtggccgg ggtcaaccgc ttcctcggcg acctggtcag gggcgagggc
     2581 gggctgcgct cggcccgtcc cggcgtgctg gtggggagct ggtcggtggc cgtggacgcc
     2641 gggcccgact cggtgcggct ggaacgcgaa ctgcaggccg tggacgcccc tgccatgatc
     2701 gccgccgtcc gcccggacat ccacttcatc cagacgaact ggcccgactg gctcaagccc
     2761 gacctggccc ccgactacat caagggctac cagccgtacg tggacgagat ccggcggagc
     2821 aacccggcgc tgccgctggg cgtgcagaac gacatcggct ccaccaccgg gaccgcgagg
     2881 gaccgcgcgt ggatcggcgc gttctccgac acggcctcct cgatggggtt ctccacctgg
     2941 acggggtacg agttctcgct ggccaaggcc atgtacaccg aggcgcccga ggtgcgcgcc
     3001 atccgctgcg accgggcggc gggcaccgtg gaggtgagct tccagaagcg gatcgacgcc
     3061 gcgtcggccg cccgcccgga cagcttcgcc gtacggacgc ccgccggacg gtcgcacggc
     3121 gtgctgggcg tcgaggtgga cggcaacatc gcgaagctgc ggctggacgg gctgccgcgc
     3181 gggcggttcg agctcgcggt gcggaacgtc agcgacaccc cgtcgctgtg ggtgttcaac
     3241 aagacgcagc agcccaacac ggtcgagccc ggctacacgg ccgagggccg ctgcacggga
     3301 tgaccgcggc gggcgcgggc cgcggggtcg ccgcggcccg ctccccccgc acggggtcag
     3361 gcgatcgaga tggctccgga cgggcagagg ttcacggcgc ggcgcacggc ctcatcgagc
     3421 tctgccggcg gctccttctg caggagcacc accgtgccgt cctcctcgct ctggtcgaac
     3481 accttgggca ccgtcagggc gcacatgccc gctccgatgc ataccgacgt gtcagcgttg
     3541 atgcgcatgg tgacctacca agtgacaggg aggcgatgga ctccgtagat gctcatgtcg
     3601 gagcgcatcg gcacctcctc gggtggtacc gccaagcgta ggccaggcag gcggcgcagc
     3661 agcgcggggt aggcgatgcg catctccacg cgggcgagct gctgcccgag gcactggtgg
     3721 atgccgtgcc cgaacgacag gtgccctccc gcgggcctgg tcaggtcgag cgtgtcgccg
     3781 ccggggaagc ggtcggggtc gcggttggcg gcgggcaggt ggagggtgac ggactccccc
     3841 gccctgacga ggtggccgtc gatctcgacg tcctcgatcg cggtcctgat gggcccgagg
     3901 tggacgatgg tgaggtagcg cagcagctcc tccaccgcgt tctcgacggc ccccgggtcg
     3961 ccgcgcatga cggcgagctg gccggggttg gtgagcagcg tgtacgtgcc cagcgcgagc
     4021 atgttcgcgg tggtctcgtg cccggccacg agcagcagga acgacacgcc ggcgagctcg
     4081 tcgtccttga gctcgccgcc ttcgatgagg ccgctgatca ggtcgtcggc cgggtgctcg
     4141 cgcttgcgcc cgatgaggtc gtgcaggtag gcgaagacgc ggccgatggc ggcgatggcc
     4201 tcctcggcgc cggtctccag gttgaccagg atcttggagt cgtcctggaa gcgggcgcgg
     4261 tcctcgtacg ggactccgag cagttcgcag atgaccagcg acgggatggg cagcgcgaag
     4321 tcctgcacca ggtcggcggg cggtccggcg gcctccagcg cgtcgagcgc ggaggcggtg
     4381 atctcctcga tcctgggttc gagcagcttc atcctgcgga cggtgaactg cccggtgagc
     4441 agcttgcggt agcgcgtgtg ctcgggcggg tcggtccgca ggaagaaccc cggcggcgtc
     4501 tggaaccccc gctcgcggag ctccttgccg gccgcgaacc gtcccgggat gggcgggtgc
     4561 atgttctcgg gccggttgct gaagcgcgag tcggccagca cggcgcgggc ggcggagtag
     4621 ccggtgacga gccagccgac gtggccgtcg gcgtacgtca tccggtgcac gccgccctgt
     4681 tgtcgtagcc gggcgacctc gtcgggagga tcgagcggcc gttcgcggac agtgggcagg
     4741 gcgggcacgg tcaggtcgga catgctgggc tcctttcctg gctcacctcc cagaaaacct
     4801 cacggaatgc aaactttcaa tggatgaaaa acatggcgcc atgcagtaca gttccgccat
     4861 ggtgggtctg cgggagcgca agaagcagcg cacgcgccgg gcgctggtcg acgccgcgct
     4921 ccggctgttc gccgacaagg gctacgagga gaccacgctc gccgagatcg ccgcggcggc
     4981 ggacgtgtcc acccgcacgt tcttcagcta cttcgccagc aaggaggacg tcctcttcta
     5041 cgacgccgag gagcggatgg agcgcctcct cgccctggtc gcctccagag ggccgcacga
     5101 gtcccccgcc gacctgctgc tgcgcctcat cgacgagatc gtctaccgct ccggcgagga
     5161 ggaggacctc acgctggagg agaccaggct ccgcatgcgg ctcatcatga gcgtgccgcc
     5221 gctgcaggcg cgggcgctgt tcctgctgtt cgacacccag ctgcggctgg ccgaggcgct
     5281 gtgcgaggcg tacccggggc ggctcggccc cctcgacgcg gccgccgcgg tgggggcgct
     5341 caccggcgcg gtgaagctgg ccgcgatggc cgccatcagc cggggcgcct cactgcagga
     5401 gatctcggac accgcgcgca gggcggccgc gctcgccgcg tccgggctgc gctccctcgg
     5461 ctgagccccg ccggttccta ccgggcggag gccagccgcg ccgcgacgac ccgcgcggcg
     5521 gggcgcggca cgtcgaggaa cgcccgcagg tcgccgccgc gcgaccgcat caggccggcc
     5581 cccgccgccg agaacagcga gagcgtgtgc cccacctggt acggctccag cccgctcgcc
     5641 cccagcgccg ccctggcctc gccgagcggc acgggccggt agccgacggg ccgcccgagc
     5701 gcctcggtga gcagcgcggc gaggtccgcg ccgcccagcg ccgtctcgcc ctccagctcg
     5761 tacgtacgcc ccgcgtgcct gcgcggcctg ccggccgcga ggtcggcgtg ggtctcggcg
     5821 gcgacgcgca cggcgacgtc cgcgaggtcg tccctggcca ccaccggcat ggcgccgtcg
     5881 gcgagcggcg ccgcgagcac gtctcccacc gtgccggcga gcgccaggtc ggccagcagc
     5941 tcggcgtaca gcccgttgcg caggaccgtg acctcgaacg gcgcctcggc gaggcgggcc
     6001 tcggtccagc ggtgcgcgag cgcgatggac agggggtcgc ccgagccggc caggctggtg
     6061 tacaccacgt gccgaacgcc cgcctcggcg gcggcccgca cgacggcgcc gtggcgggcc
     6121 agcacgacgt cgtcctcggc gtacccggcc gagacgagga ccagcacgtc caccccggcg
     6181 aacgcgccgg gcagcgtccg cggcctgtcg aagtcgacca ggcgcacccc gcccgccccg
     6241 ccgtcccccg cgcgggagcc gcccgccacc tccaggccgc cgtgcgcgcg cagaccgcgc
     6301 aggatcgagc cgccgagcgc gccggaaacc ccggtcacca agatcatgat tggacacccc
     6361 tactgtggac gccgtggatg ggtcggtcgc cccaggatgg gggcggcacg ccactcggcg
     6421 taaggaggca ctctcatgtc agtcgggcac accgcgataa ccgcccagct cagcgggcag
     6481 gccgcggcgc aggcggccgg gccgtgcggt gacgacgact gcgggatccg cgacgtcctc
     6541 gaccgtctcg gcgaccgctg gacggtgctc gtggtcgtgg agctcgccaa gggcgtgcgg
     6601 cggttccgcg agctgcagcg ggcgatcccc gggatctcgc agcgcatgct gacgctcacc
     6661 acccgccgcc tgtgcaggga cggcctggtg gagcgcacgg cgtacccgac gatcccgccc
     6721 caggtggagt accggctgac ccccatgggt cacagcctgg cggaggccgt ggcgagcctg
     6781 gccgactggt cgcgcggcca caaggacgcc atcgccgcgt cgcgggcgcg ctgggacgcc
     6841 gagcggacgg gctgagcccg cccccgcgcg gggcgggcgg gcgcgtcagg cgcggtggaa
     6901 gcggccccgg acgtgcaccg gcacgccggt gccgagcagg ccgggcagca cctcggcgac
     6961 gttcatcggc aggcggacgc agccgtgcga ggccgggtac agcggcacgg acagcgcgcc
     7021 gtggaaggcg atgccgccgt tgaagaagat cgggttgtac agccgcccga ggtacgaggt
     7081 gtgccagccg ctcctgcgcc aggtcgtgcg gaagtcgccg gtgggcgtgc tggccgagcc
     7141 gcagaagcgc tgcttcctgc cctgccaggt ggcggtctcg cagtacggca cgccgctgcc
     7201 ggacgagatg tggctgatca gcttgggccg gccgtcgacg tacaggacca tgacctgctt
     7261 ggtcaggttg acctcggcgc gggtgggcct gccgttcttc acgagcacct gcggcgcctt
     7321 cggccgctcc agcgccgccc aggtggccct ggtgatcgtg ctggtcggcc tgatgccgtt
     7381 gaccttctgg aacgcccaga cggcggcgag ggtggtgccg ccgtacctgc cgtcggcctt
     7441 gccggggagg tagcccaggc cggcgaggcg gttctgcatc cagaccacgg cctcgcccct
     7501 ggctccgagc ttcagcgtct tgacgggcgc ggcggcgaag cgggcgtact ccgcctgctg
     7561 gacggtcgcg gagcgagcgg gggtcgcgga gccggtgacg gtcgccgcgg cggccggacc
     7621 ggcgagcgcg ccggccccgg agagagtcac ggagaaagtc acggcgccgg ccgtcaggag
     7681 agcggccatc gcggggagcg gtcgcactcg aaccacctct catctgcccg gaatgttcaa
     7741 aagaccctac accagcgtca cgatcaggta atcgggccgt caacctgttt agagtgtccc
     7801 catgtctgag aagttgccgg ggaacgcggt gaaagtcgac gcggcgctgc gcgccctggg
     7861 cgccacgggc gagatcgtgg tgctgccgga gaaggccccg acggcggcca ccgcggccgc
     7921 ccagctcggc tgcgaggtgg gagccatcgc caacagcctg atcttcgacg ccgacggcgc
     7981 gccgctgctg gtgctgacca gcggcgcgca ccgcgtggac gtggggctga tcgcgcgcac
     8041 ggccggggtg accgaggtgc ggcgggccac gcccgagttc gtccgggccg ccacgggcca
     8101 gccgatcggc ggtgtcgccc cggtcggcca ccccgccccc gtgcgcacgc tggtggacaa
     8161 ctggttgagc aagcacgagg tggtctgggc ggcgggcggg cacccgctga cggtgttccc
     8221 gacgacgttc gacgagctgg tgcggatcac cgggggaacc ccggtcgacg tcgagtaatc
     8281 tgcgacgggt gatcgagttt cgcggggcgg gtccggccgg tttcaccgaa cggctggaca
     8341 ccgtgatcga catttacacc gcagccatgg atccgcctcg ggagcagatc gccggccgta
     8401 agaccatcat gcgaaatcac agcacatatc cgtatttcca gtgctatttc gcagaattgc
     8461 gggattctcc gggtgagggc ggcgcgcccc ggccgccgat cgtcgtcggc ttcgcgtacg
     8521 gcttccgcgg cgccccaggg cagtggtggc acgacgtggt gtaccgcgcc ctggaggagc
     8581 gcgccggcga ggaggccgcc cacgcctggc tcgggttcgc cttcgaactc gccgagatcc
     8641 acgtccaccc cgaccaccag ggcaggggca tcgggcgtgc catgatcagg acgctgtgcg
     8701 cgggccgccc cgagcgctcg gccgtgctct cgacgcacga ccgccccacc gccgcccgcc
     8761 acctgtacgc cgccatgggc ttcaccgacc tgctggccag gttcgtcttc cccgggggct
     8821 acgaggagta cgcgatcatg ggcaggccct tgccgctcga ttgatcttta ccttgcccgc
     8881 cgtttgcccg ggacggcctg cgggcacgaa tcatgacgga aagtaaccgt ctcatgaccc
     8941 ttcggggtcg ccccccgccc gtcacccgcc cggtccccct gcggacgggt ggcgggctcc
     9001 cccatcacct gggtcaggcg ggctggcgca cgtccagccg ctggaacacc tcgcgcgagg
     9061 cgctcgacct gttgaacgtg atgaagtgga tgcccggcgc gccctcgtcg agcagccgct
     9121 ggcacagctc cgcgccgtgc tcgatgccga gcgcccgcac cgccgccggg tcgtcggcga
     9181 ccgcctcgaa gcgggccgcg acctcggccg ggaacggcgc ccccgacagc tgctcggacc
     9241 tggcgatcgt gctcatctgc gtgacgggca tgatgcacgg gatgatcggc gtgtcgcagc
     9301 cctgcgcctc gacccggtcg cgcagccgca ggtagtcgtc ggccctgaag aacatctgcg
     9361 tgatcgcgaa gtcggccccg gcccggcact tgcgcacgaa gtactcggtg tcggactcga
     9421 tggacggcga gcggggatgc ttgtacggga acgccgccac gccgacgcag aagtcgccgg
     9481 actggcggat gaggcggacc agctcctcgg cgtagagcac gccctccggg tgctggaccc
     9541 actcgcccgt gacgtcgccg ggcgggtcgc cgcgcaccgc gaggatgttg cgcacccccg
     9601 cgtcggcgaa gcggcccacg aggtggcgca gctcgcgcac cgagtggttg accgcggtga
     9661 agtgcgccac cggcgtgagc gtggtgtcgt gggcgaggcg ctcgacgatg tcgaccgtgc
     9721 ggtcgcgggt ggagccgccc gcgccgtacg tgaccgacac gaaggtggga cgcagggact
     9781 cgagctccct gatggcccgc cacagctgac gctcgccctc gtcggtcttc ggcgggaaga
     9841 actcgaacga gaacgaccgg ccgccggagg cgagcagttc gcggatcgtg gggacacgat
     9901 cggggaggac cgaaggtcga ccaagcgcca tgacccaagg ttacgccaca agcccggacg
     9961 ggagatccga gactcgcggc ctgaccatta cgctcaatgc atgaccaatg acgcagccag
    10021 catgggcacc ttgcgcgcgc acgtcgagca ggcactcagg gagttcgtcg aacggcagct
    10081 ccccgcatca ggcgaccccg atctggagcc gctgcgcacc gccgcccacg agctgctggc
    10141 gggcggcaag cggctgcgtc ccgcgttctg ctactggggc tggcgcggcg ccgggggctc
    10201 cgacgtgccc gagatcttca ccgccgccgc gtcgctggaa ctgctgcagg ccagcgcgct
    10261 ggtgcacgac gacgtgatgg acaagagcga cctgcgcagg ggcatgccgt cggcgcaccg
    10321 ccgcttccag acgctgcacg agaaggcggg ctggcacggc tcggccgagc agttcggcga
    10381 gggcgcggcg atcctgctgg gcaacctgct gctcatctgg tcgggcgaga tgtggcgcac
    10441 ctgcggcctg ccggcgcgct cgctggccgc cgcccagccc gtgcacgacc acatgcggac
    10501 cgagctgatg tgcgggcagt acctcgacct gctcgaacag gcgcacggcg agaacacctt
    10561 cgactccgcg ctgcgcgtcg cgctgtacaa gagcggcaag tactcggtgg agcagccgct
    10621 gcggctcggc ctcgcgctgg cggcgggcga gctgctgccg tggatggacg agctgtgcgc
    10681 cggctacggg cggcgcgtcg gcatcgcctt ccagctccgc gacgacatcc tcggcgtgtt
    10741 cggcgacccg gcgcagaccg gcaagcccgc cggcgacgac ctgcgtgagg gcaagcggac
    10801 gatgctcatc gcccgcaccc tggccgccgc ctcccccgcc gcggcggcga cggtgcgccg
    10861 cctgctcggc gaccccggcc tggacgcgga cggcgtcgcg gcgctgcggg agatcatcga
    10921 cggcaccggc gcgctgcgcg cgtgcgagga cctgatcggc gagtacctcg gggagggcct
    10981 ggccgcgctg gagggggcgc ccatcacgca ggaggcgcgt acggcgctga ccgagctcgc
    11041 catcgccgcc acctcccgca ggacgtgacg cccgcccgcg aagaaggccc gcggagaagg
    11101 agaaggccgg ccccggtggg ggccggcctg aggacgggga gcacgcggcg cgggccggcc
    11161 gcgtgaacgg gggtcagcgg cccggccgcc gcgtggtcgg gctcagcggg ccagccgctt
    11221 ggcgaactcg gccgcggccg cgccggggtc gtcggcctcg gtgatcgccc gcacgacgac
    11281 cacccgggtc acgccgtacg ccatgacctc gtcgagggtg gtcaggtcga tgccgccgat
    11341 gccgaaccac gggcggtcgg cgcccagcga cgcggcgtgc ctgagcagcg ccgggccggg
    11401 cgcgtggcgg cccggcttgg tcggcgtcgg ccagatcggc ccgcagcaga agtagtcgac
    11461 gccctcctcc acggccgcgg cggacgcctc ggccatggag tgggtggacc gtccgatgag
    11521 gaggtcgtcg ccgagtatct cccgggcgac ggtcacggga aggtcgtcct gccccaggtg
    11581 gagcacgtcg ggccgggcgg cgtacgcgat gtccgcccgg tcgttgaccg cgaggagcct
    11641 gccgtgccgg tcgcaggcgt cgcggaagac ctcgaggagg gcgagctcct cgcgcgcctc
    11701 caggcccttc tcccgcagct ggacgatgtc cacgcccgcc gccagcacca cgtcgaggaa
    11761 ctccgccagg tcgccgcgct cgcggcggcc gtcggtgcag aggtagaggc gggccccggc
    11821 gagcgcctca gaaagcgagg gcttgggcac gccgcttgac ctccgtgtgg cgacccgcct
    11881 tgatcgcgtc catcggcgtg ccgggcaggg agtcgtctgg agtgaagagc cagcgcaggg
    11941 cctcgacgtc gtcgtagccg gcgtcctgga gcacggtcag cgttccgggc agccccttgc
    12001 gcacatcctt tccgtcgagg aagaccgcgg gcacgtgcgg cccgccgctg ccgcggcgca
    12061 cgccgagcag cttgtggtcc ttgagaagtt gcttgacccg gcccagcgga agctcgaggc
    12121 gcgcggccac ttcggggagg ctgagccact cgtcgacgag ctgctcggtc tcccggtcga
    12181 tctgtgcaac agaaagcgtc acggaaccac aactaccaca tgccgtggtg gctcaaacaa
    12241 cacagtctcg cagggcgaca tctggatcta ccaggcgttc cgcgtcgagg cggtgccccg
    12301 cctcgatgat gcgccggccc tggacgagat cgcgcggacg gtcgaccgcg agaaccgcgg
    12361 cgagggtgtc gccggccgtg agccagcagg ccgaccactt gccctcgggc tcgccacggt
    12421 acaccagccg ctcgcccgcc gggtggtgcc cggcgtactg cagcatgtgc ccgaactgct
    12481 cggaccagaa gtacggcacc gggtcgtaga cggcgtcctc acccagcagc gtcgccgcgg
    12541 cgacctcggg ggcgtgcagc gcggtgtccc agtgctccac ccgcagccgc gactggaagc
    12601 ggcgcgacca ccaggcggcg cagtcgcccg ccgccacgac gttcggcagg gaggtgcgca
    12661 ggtgctcgtc ggtgacgacg ccgttgtgca ggtcgatctc ggcgctcgcc agccactcca
    12721 cggccgggcg caccccgatg ccggtgacga cgacgtgcgc gtcgacgaac tcgccgccga
    12781 ccagctcgac gccgccctcc cgcacctcgt ggaccatcgt gccgaggcgc aggtcgacgc
    12841 cctcgtacca gccggtggtg cgggcgccga gctcggggcc gagcgcgtgg gcgagcggcg
    12901 cctcgcccgc ctcgatcacc gtcaccgcgc agccggcgcg ccgggcggcg gtggcgacct
    12961 ccgcgccgat ccagcccgcg ccgatgaccg ccacccgcgc gccgggcacc agcagggcgc
    13021 gcagctcgtg ggcgtcgtcg agggtgcgca ggacgtgctg ggggccgtcg cccggcagcg
    13081 tgatcggctc ggcgccggtg gcgatgacca ggccgtcgta gcccagatcg ccctcggtgg
    13141 tctccacggc gccgtcgcgc agcgccttgg cggagacgcc cgcccagaag tcgacctcga
    13201 gcgccgacag gtcggtgtcg acgaagctgg agtcggcgtc gccgagcagc accgccttcg
    13261 acagcggcgg ccggtcgtac ggacggtgcc gttcctgccc gatgagcgtg atccggcccg
    13321 cgaacccgcg cgcccgcagc gcctcgacac tgcggacgcc cgcgagcccc gctcccacca
    13381 ccacgacatg cttcacgccc atgaccataa cggggcgggt gcgggcgcgt cgcggggagg
    13441 ccgtaggctg ggggccgtaa gacgcgcggg agtccggccg taaccgggct gagaggaagg
    13501 cttcaccagg gccttcgacc gcaagacacc tgatccggat catgccggcg aagggagcgc
    13561 gcatggacgt cacggatgtc atggatgtcg tcgtgggcgg cggggtcgtc gggctggcgg
    13621 tcgcctggcg gctggcccgc gaggggcggc cggtgacggt ggtcgatccc gccccggcct
    13681 cgggggcctc gcacgcggcg gccggcatgc tcgcgccggt cagcgaggtg acctacacgg
    13741 aggcgccgct gctgcggctc ggggcggcct cgctgcgccg ctggccgtcc ttcgccgcgg
    13801 agctggcggc cgacgcgggg ctcggcgacc cggcgcgggc gctcgacctg cgcgccgacg
    13861 gcaccctcga cgtggcgttc ggcgccgacg acctggcggc gctcgacgag ctggccgcgt
    13921 tcatggagaa gctggagctg ccggtcgagc ggctgaccgg ccgcgagtgc cgccggctgg
    13981 agcccatgct cgccccctcg gtgcgcggcg ggctgctcgc gcccggcgac gcctgggtcg
    14041 atccgcgccg cgtcaccgcg gcgctgctgg ccgccctcga ccggctcggc gtgccactgg
    14101 tgcgggcgcg ggtggccggc ctgctgcgcg acggcgaccg ggtcaccggg gtgcggctgg
    14161 ccgacggccc cggcgggacc ggtctccagg agagcggccc tcgggagatc agggcggcga
    14221 acgtcgtgct cgcgacgggc gcgtggagcg gcgagctgct cgacctgccg gtgcggccgg
    14281 tgaagggcca gatcatgcgc ctgcggtcgc cgcagccgct gctcggccgg tgcgtgcgcg
    14341 ggatggtcca cggcgcgccg gtctacctgg tgccgcgggg cgacggcgag ctggtgctcg
    14401 gggccacgca ggaggagctg ggcttcgaca cgcgggtgac ggcgggcggc ctgtacgagc
    14461 tgctgcgcga cgccagggag ctggtgcccg gcgtgacgga gctggaggtg gcggacatgg
    14521 tggcggggct gcgccccggc acgccggaca accttccgct gatcgggccc gccggcgtgc
    14581 cggggctggt cctcgccacc ggccaccacc ggggcggcgt cctgctcacc ccgctgacga
    14641 cggacctggt gctggacgcc ctgacggccg cgggcggcgg ggcgcgtgag acggagacgg
    14701 gcgagacggg tgagctggcg gagctgtgct cgcccaggag gtttcaggaa acttcatgat
    14761 tgtgatgatc aacgggaccg cgcacgaggt ccccgacggc acgaccgtgg cccaggccgt
    14821 acggaccctc acgacggcca ccgccggcgt ggcggtggcc gtgaacgacg aggtggtcac
    14881 gcgcggctcg tgggagacga cggcgctgag cgacagcgac cgcgtcgagg tgctgacggc
    14941 ggtgcagggt ggatgagctg accatcgccg gggagtcgtt ctcgtcccgg ctcatcatgg
    15001 gcaccggcgg cgcgccgtcg ctggacgtgc tcgaccaggc gctggaggcg tcaggcacgg
    15061 agctgaccac ggtggcgatg cggcggctcg acccggcggc gcgcggctcc gtgctggacg
    15121 tgctgcgcaa gcgcgacatc aaggtcctgc cgaacacggc cggctgcttc accgcgggcg
    15181 aggcgctgct gacggcccgg ctggccaggg aggccctcgg cacgaactgg gtcaagctgg
    15241 aggtcatctc cgacgagcgg accctgctgc ccgacccgat cgagacgttc gacgcggcgg
    15301 agcggctggt ggccgagggg ttcgtggtgc tgccgtacat cggcgacgac ccggcgctgg
    15361 cgcgccgcct ggagcaggcg ggctgcgcgg cggtgatgcc gctcggcgcg ccgatcggct
    15421 cggggctcgg catccgcaac ccgcacaaca tcgagctgat cgtggaggcc gcgtcggtcc
    15481 ccgtgatcct cgacgcgggc atcggcaccg cctccgacgc cgcgctggcc atggagctcg
    15541 gctgcgacgc ggtgctgctg gcgacggcgg tcacgcgggc gcagcggccc gacacgatgg
    15601 cggcggccat gcgggcggcc gtggaggcgg gcaggctcgc ccggctcgcg ggccgcatcc
    15661 cccgccgccg ctacgccgag gcctcctccc ccatgacccc ctgacgaccc ccgcgccggg
    15721 gggcggcggc tcccccgccg ccggcccccc gaaaccccgg aacaccttgt gcgtcgggca
    15781 cgtatcggca tcggtttcgg gtggcgaggc ggtgccagtg ccgaaacgcc cgtaaactca
    15841 gcggggtgga tacgacgaca gcggaccccc tggtcgggcg gcttctcgat gggcgctacc
    15901 gcatcgagag ccggcttgcc cggggcggga tggcgaccgt ctacctggcg ctcgacgtcc
    15961 ggctcgaccg gaccgtcgcg ctgaaggtca tgcatcgctc cctcgccgac gaccccgcgt
    16021 tcgtgcgcag gttcatcggc gaggccaagt cggtcgcgag cctctcgcac cccaacgtcg
    16081 tgcacgtctt cgaccagggc accgacggcg agatcgtcta cctgtcgatg gagtacgtgc
    16141 cgggcaggac gctccgcgac atcctgcgcg agcgcggccg catgccggcc cgcgaggccc
    16201 tggagatcat gatcccggtg ctcgccgccc tgggcgcggc gcactcggcg ggcatggtcc
    16261 acagggacgt caagcccgag aacgtcctga tgaccgacga cggccgggtc aaggtcgtgg
    16321 acttcggcct ggccaaggcc atcgaggcca ccaaccagac ccgtacgggc gtgctgatcg
    16381 gcacgatcgg ctacatggcg cccgagcagg tcatgacggg cgccgccgac gtccgcagcg
    16441 acgtctacgc cgcgggcatc atgctgttcg agctggtcac cggccagcag ccgtacgacg
    16501 gcgagacgcc gatgtcggtc gcctaccggc acgtccacga caccgtgccg ctcccgtcgt
    16561 cgatcgtgcc cgacgtgccg ccgccgatcg acgccctggt cgcccacgcc accgcacgcg
    16621 agccccacga ccgcccggcc gacgcgtcgg cgatgctggt cgaggccgtc gagacgcacc
    16681 gggcgctgcc gcggctgacc acccctcccg cgtcccactc gcagccgcac gcgaggccgg
    16741 cctccggcgc gcactccgtg ccgcagccga cccaggccct cccgcaggcg gcgcactcgc
    16801 agccgcacgc ggcccattcg gcccactcgg ccccctcggc gccgatggcg gcgccgagcc
    16861 acaccatgat ccagccgcgt gtcgaggcgc ccgagcgccg gcgcggcttc cggccgagct
    16921 ggttcctcgt ggcgctggcc gccgtcatgg cggtggcggt cggcgtcacc gcctggttct
    16981 tctctcgccc caccctcgtc atcgtccccg acctggtcgg caaggacatg gcgctggccg
    17041 agcagagcgc cgccaggctg gggttcaagg tgcgcaaggc cgagggccgc tacgacgaga
    17101 aggtgcccgc cggcctggtg ctgagcatgg agccggtggc gaagaccgag gtggagaagg
    17161 gcacccagat cacgctggtg ccctcgctcg gcgccaagcg catcgtggtc ccgaacgtgg
    17221 ccggcatgag ccagaacgac gcccgcctgg ccatcgccgc ggccggcctc accgtgggcg
    17281 cggtcaagcg cctgcccgac gagcaggtcg agcgcgacaa ggtgatccgc acctcgccgc
    17341 aggtgggcga gaaggtcaag gaaggcgcga aggtcggcat ctacgtcagc gcgggcctgg
    17401 tgatgcccga cgtgagcaac atgccgaagg acgaggcggc cgcctacctc ggcggccggg
    17461 gcttccaggt gcaggtcaac gaggtcgacg acgacgccga gccgtgcacc gtcatcgcgc
    17521 agtcgccgaa ggccaggtcc gaggtcgaca agggcgccgt ggtgatggtc accgtggccc
    17581 gttgccagac cgacttctgg cagtggttca acggcgacaa cgaggcccgc gacgaccagg
    17641 acgtccgcct ggtgcccgcg gtgatcggca agaacgtcaa ggacgccaag gccgacctgg
    17701 aggcgctcgg cttccacgta cgggtgaacc ggctgagcaa cggcggcgtg gtgcagttcc
    17761 agcggccgct gccgaacagc gagcggccgc ccggcaccac ggtctacctc tggcagtgac
    17821 cccgtgatcc cgcccgcgcg gcgggcggga tcaggcggga tgcccgaaca ggcgcaccct
    17881 ggccagcccg ccgtcggggt ggatgtccac gcgcaccgac gccacgggtt cggtgtggcg
    17941 cagcgtgaaa cggtggagcg tgtcgggccg cagcggggtc cgcggcagca gctcgatctc
    18001 gcggtcgtcg ccgccggggc ccggcgggag gccggtcagc gccacctcgg cgggggcgtt
    18061 gtgcacgagg ttggcggtgt cgatctcggc gagcctgacg cggccgggcg cggccagccg
    18121 gatgaccagc cactcgttgc ccggctcgcg gcggcgcgcg gtctcccagc cctccgcctg
    18181 gtggcgggcc aggccagggg cgatgacgtt gtgcggcgcc gagtagaagg cgtcggagca
    18241 ggcggtgacg agcccgccgt tggccagcgc cgccaggtcg aggccgagcc cgtcgtagac
    18301 ggacaggtcg gcgcggggct cgccgtgcac gcgcagcctg gccacgccgc cgtcggggtc
    18361 gatcgcgagc cgtacgtggg tgtacctggc ctcgtcggcg acggggaaga ggtgctcggc
    18421 gtcgccggtg agcgggctcc tcggcacgag ggtcgtccag cgggcggcgg ccagctcgga
    18481 cggcgacggg tggccgtccg cctcggtggc ctcgacccag gcgtggggcg ggtggttgcc
    18541 cgtgaaccag gcggtgtcga tggcgacgcc gcggacgacg ccgggcaggc cgagccgtac
    18601 gagcgcccag tcgtggccgg gtgagcggcg gcggcgcgtc tcccagccgt cgtagacctg
    18661 ccccttgccg ccgaacgtct ccggggtgaa gccggggcgg cccggcctga tgaggttctc
    18721 cttctcggcg aacgactcgt cgctcgcggc gacgaccgcg cccccgaacg tccgcagggc
    18781 caggtccggc aggctggtga agtccggcat ctactctgct cccaggagga atcgcccgtg
    18841 tggtaggccg tccaccggcc tgccgcgcag ccaggtggtc aggacggcgc cccgcagggt
    18901 ccggccgtgg tacggggtga cggggttcct gtggtgcagt cgggcggcgt cgacccggac
    18961 ggccgcgtcc gggtcgaagg ccacgaggtc ggcgtcgcgg cccggcgcga tcgcgccctt
    19021 ccccggcagc ccggcgagct ccgcaggccg ctccgccatc cagcggacga cgtccggcag
    19081 cccgaagccg cggcgcgacg cctccgtcca gacggcgggc aggccgagct gcagtgagga
    19141 gacgccgccc caggcggcgg cgaagtcggg caccttgagg tcgggcgtcg agggcgagtg
    19201 gtcggagacg acgcagtcga gcacgcccct cgccaggccg tcccagagca ggtcccggtt
    19261 ggcccgcgag cggatcggcg ggcagcactt gtacgcggtc tccccctcgg gcacctgctc
    19321 ggcgtcgagg gtcaggtagt gggggcaggt ctcggcggtg atgcgcacgc cgcgcgccct
    19381 ggccgcctcc aggacgtcca ggcacgcggc gctggagacg tgcaggacgt gcacccgcgc
    19441 cccggtccgc gccgcctcct cggccagcga ctcgaccgcg ccgcgctcgg ccgcgtccgg
    19501 ccgcgactcc aggaactggc ggtaggaggg gccgcccggc tcgcgcagca gcgccgggtc
    19561 ctcggcgtgc accacgacca gcccgccgaa cccggccgcc tccgtcagcg tcgcgcgcag
    19621 ctccgccggg ctcagcggcg ggaactcgtc cacccccgac ggcgacatga agcacttgaa
    19681 gccgcgcacg cccctgtcgt gcagcggcct cagctcctcg acgttgcccg gcacggcgcc
    19741 gccccagaac gcgacgtcaa cgtggcagcg gccctcggcg gcccgcagct tcgcgtccag
    19801 ggcgtcgggc gagaccgtgg gcggcagcgc gttgagcggc atgtccacga tcgtggtgac
    19861 gccgcccgcc gcggcggccc tggtggccgt ctcgaagccc tcccagtggg cgcggcccgg
    19921 gtcgttgacg tggacgtgcg tgtcgaccag gccgggcagg agcgccagat cgcgcagatc
    19981 gacgtcctcg ggggcggcga gcggcgcgcc gtacggctcg accgcggcca cgcgcccgcc
    20041 ccggacggcg acggccgcgg cccgctcccc ctcgggcgtg accacccggc gggagcggac
    20101 cacgagctcg tacgcggtca ccgcaccgcc cccaggtgtt cggcggcgac gcgcgccgcg
    20161 aggcgttcga gcagctcccc cgcctctgcc atgcagcggg ccgggtcggg ctccaggtcg
    20221 gtgagggcgt aggcggcctc gatgccgacc gccttcagct cctcgtcggt gagcgtgcgc
    20281 cgcccgcaga ccgcgaccac gggcaccccc gccttcgcgg cggcctgggc gacgccgacc
    20341 ggggccttgc cgcgcagcga ctgctcgtcg agcgagccct cgccggtgac gaccaggcgg
    20401 gcgtcgcgga ccagctcgcc gaagccgagc aggtcgagga ggtaggcgat gccaggacgg
    20461 aggtcggcgc gcaggaaggc cagcgcggcg aagccgatgc cgcccgccgc gcccgcgccc
    20521 ggcgcgcccg ccacgcccat cggccggggg atgtcgtcgt gctcgaccgc gcccaccagc
    20581 ccgtgcgtac gggtggcgac ggcggcgaag cgggacagcg ccgactccag gaccttgacg
    20641 tcgtcggggg tggcgccctt ctgggggccg tacacggcgg cggcgccgaa cgggccgagc
    20701 agcgggttgt ccacgtcgct cgccaccacg aactccacgc cggagacgtc caggaagccg
    20761 gtgacgtcga tccggtcgag cgccttcagg gcggcgccgc cgggcggcag gtcgtgccct
    20821 tcggcgtcga gcaggcgcgc gccgagcgcc tgcaccatgc ccgcgccgcc gtcggtggtg
    20881 gcgctgccgc ccagcccgag caccacgcgg cgggcgcccc gccgcaccgc gtcggcgacc
    20941 agttcgcccg cgccgtggct ggtggcggtc agcggctcag gggcgccggg cagcctgcgc
    21001 agccccgacg actcggccac ctcgatcacg gccgtgggga cgccgccgcc ctccgccgac
    21061 ggcgcgcc
//
