LOCUS       NZ_BBZG01000001        12800 bp    DNA     linear   CON 12-AUG-2024
DEFINITION  Nonomuraea pusilla strain NBRC 110462, whole genome shotgun
            sequence.
ACCESSION   NZ_BBZG01000001
VERSION     NZ_BBZG01000001.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nonomuraea pusilla
  ORGANISM  Nonomuraea pusilla
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Hosoyama,A., Fujita,N. and Igarashi,Y.
  TITLE     Draft Genome Sequence of Nonomuraea sp. TP-A0861, a Producer of
            Myxochelin A
  JOURNAL   Genome Announc 3 (6), e01430-15 (2015)
   PUBMED   26659677
  REMARK    DOI:10.1128/genomeA.01430-15 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746196)
  AUTHORS   Hosoyama,A., Kimura,A., Ichikawa,N. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2015) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, Biological
            Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.8
            Genome Coverage                   :: 107x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417755.1-RS_2024_08_12
            Annotation Date                   :: 08/12/2024 01:46:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,307
            CDSs (total)                      :: 8,242
            Genes (coding)                    :: 8,159
            CDSs (with protein)               :: 8,159
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 21 of 83
            Pseudo Genes (incomplete)         :: 72 of 83
            Pseudo Genes (internal stop)      :: 5 of 83
            Pseudo Genes (multiple problems)  :: 15 of 83
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:25:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 62337
            Orig. end                         :: 75137
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBZG01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12800
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="1"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12800
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12800
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7800](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7800
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    73..86
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            627..1010
                     /locus_tag="ASC57_RS00280"
     CDS             627..1010
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895609.1"
                     /locus_tag="ASC57_RS00280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_055500933.1"
                     /transl_table=11
                     /translation="MNSSSGVVAALDGCVVQEPDADRVAATRDRLVTPEEATRLAELFR
                     LLGDPTRARLLYALLEAGELCVCDLADAVEVTDTAVSHALRLLRTAGIVASRRAGRMIY
                     YRLADAHVRMLLDLSREHLKETG"
     gene            1007..1960
                     /locus_tag="ASC57_RS00285"
     CDS             1007..1960
                     /GO_function="GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852194.1"
                     /locus_tag="ASC57_RS00285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_055500934.1"
                     /transl_table=11
                     /translation="MSAGHGHGHGHGHGVSANADRRYLAGALALILAYMAAEVVAGIAA
                     NSLALISDAAHMLTDASAIALALIAMRIAARPARGAYTFGWKRAEILSAQINGLTLLLL
                     VAYFVYESVRRLAEPPEVQGPVVVVTAVAGIVVNAAAAWLLSRADRRSLNVEGAFQHVL
                     NDMYAFIATAVSGVVVWLTGFTRADAIAALVVAALMLKAGWALLRDSARVLLQAAPSDL
                     DPDEIGRVLAADPDVVEVHDLHVWTVTSGYPTLSAHVIVARGSDCHRVRTRLADLLHER
                     FAIGHTTLQVDHGEPDRERHCVDPHGPRHLGRTPGT"
     gene            1978..3171
                     /locus_tag="ASC57_RS00290"
     CDS             1978..3171
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544787.1"
                     /locus_tag="ASC57_RS00290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="WP_055500935.1"
                     /transl_table=11
                     /translation="MATRDPRWVFKDRPSFALITGLVVTGICALVSFSFDVLNGEPVQF
                     FIALVLALAPVPLLLAAVLALDRMEPEPRSNLAFAFAWGAGIAVLVAGVVNSLNLHYII
                     DTAKLSEASARNIAATFGAPVVEETMKGLVLLGLLRFRRAELDGPTDGIIYASMVGLGF
                     AMSENVSYYIAALNSANGSGVRDLAVTVVLRGILSPFAHPLFSSMIGVGVAYAALRNGP
                     DRVFVVLAGWSGAMVLHGLWNGLASYGGLPGLVTAYLTLLVVLVVLIGVVFRDRRRIVA
                     LIQRYLPPYEPTKLVGEADIHMLSTISRRRRARQWAKAHGGKQGLRAMRDYQLAATELG
                     LLHERAARHVVDEQTFHEEQRALLECMSAARAGFPPPVPVRPTRTRRRTPPPGYAPG"
     gene            complement(3156..3878)
                     /locus_tag="ASC57_RS00295"
     CDS             complement(3156..3878)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892479.1"
                     /locus_tag="ASC57_RS00295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="WP_236714527.1"
                     /transl_table=11
                     /translation="MLGDVRPDRRTLRRAYQGLVLLSVLALAPMTWAWLTSSGHRVTAG
                     APGWLERTPPAQAALVLGAGLAAGRPSPMLARRLDIAADLYRAGRVRALLLSGDNSTTA
                     YDEPGAMRDYLRAKGVPDAAMVLDYAGFDTWDSCVRARRVFGAERVTVVTQVFHLPRAV
                     ALCRTAGLEAFGAGDDSSRAFAGPTYVYAARELLATAKAFADARVLHSDPVYPGPREVS
                     LTTILSRPGPPPGAQPGA"
     gene            complement(3941..4912)
                     /locus_tag="ASC57_RS00300"
     CDS             complement(3941..4912)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544789.1"
                     /locus_tag="ASC57_RS00300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="heme A synthase"
                     /protein_id="WP_055500936.1"
                     /transl_table=11
                     /translation="MKVPADHPNPVVRSLLSFWTPTAESMRGWALASVVVNAGIAVTGA
                     GVRVTGSGLGCPTWPRCTPDSFVPVAHPETSMLNMAVEFGNRLLTFLVLAVGVACVVSA
                     MRMAPRRVSLVRLAWLQPAGVLAQALWGGLVVRTALNPFTVGMHFLISIGLVAACWMLY
                     ARSGEGDAPPVRLAHRDIRRLGHVLVGAVSVLLVLGVVVTGTGPHSGDLRASRFDFDME
                     AVARLHADVVYLVVGLTFALLLALHVTDAPRAARRAALALLGVELAQGVIGYTQYFLAV
                     PAALVLLHVFGSTLVWIATLRTVTSLRTRQPVPSPAQVPAPV"
     misc_feature    4947..4962
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 16.97"
                     /tool="antismash"
     gene            5001..6140
                     /locus_tag="ASC57_RS00305"
     CDS             5001..6140
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892477.1"
                     /locus_tag="ASC57_RS00305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_055500937.1"
                     /sec_met_domain="DAO (E-value: 4e-66, bitscore: 221.6,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDVIVVGAGMVGAACAYYAARAGLDVTVVDRGPVAGGTTGAGEG
                     NVLVSDKEPGPELELALLSNRLWHSLAEAGDFEFEPKGGLVVAETEEVQRALVGLAGKQ
                     GVEHAVVAASELRDLEPHLAEGLAGGVLYPQDAQVQPMLAAARLLELGARSYGHGALRL
                     RLGVTVTGFVRSGDRVTGVRTSQGDILGGAVVNASGTWGGEVAALAGVHLPILPRRGFI
                     LVTEPLPPMIRHKVYTAAYVTNVASDSAGLETSAVVESTQAGTVLIGASRERVGFDRTT
                     SVPVLARLAAQAVALFPALRDVRAIRSYHGFRPYCPDHLPVIGPDPRAPGLYHACGHEG
                     AGIGLAPATGHLMAQLLTGAAPDLDPQPFRPDRFPEEPQ"
     gene            6137..6385
                     /locus_tag="ASC57_RS00310"
     CDS             6137..6385
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623950.1"
                     /locus_tag="ASC57_RS00310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_055500938.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.7e-24, bitscore: 83.3,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEPFTITVDGRQVAVTPGQTIGAALHAAGIRSWRSTRFCGRPRG
                     LFCGIGVCFDCLVSVNGRPAERACLREAAPGDEVTTS"
     gene            6382..7800
                     /locus_tag="ASC57_RS00315"
     CDS             6382..7800
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593033.1"
                     /locus_tag="ASC57_RS00315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="WP_055500939.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 4.2e-22, bitscore:
                     76.4, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 1.3e-06, bitscore:
                     26.3, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGVRHDLVVVGGGPAGVAGALTAALAGLRVALVDSSARLGGQYF
                     RHSRAAQDAPGGAPELDGFLRRGRALAERADLLLGHRVWHVSREDGGLAVHCVTPDEQP
                     LTVRGARLLIATGAHDRVVPFPGWDLPGVLTAGGAQALLKGSGVLAGRRAVVAGTGPFL
                     LPVAAGLAGAGADVLAVYEAARGLGLARHPVLALGKAGEAAGYALALARHRVPYLTRRA
                     VVAAHGDGALEAVTVARIDAGWNVVDTRVVECDTLAVGHGFVPQIELGVELGCGTRADT
                     DGSPVLVVDAAQRTTVPGVWAAGEPTGVGGWRLAEVEGRVAGHAVAAEHRASSRASTGA
                     PAVSGGGHGAPRALLARRRGMRAFAEALQRAYPVAPGWQGWLRDDTLVCRCEEVPVSRV
                     REAQRLGATDARSIKLLARPGMGWCQGRICGYAVSCLAGEEPRPPKRPIAQPITLGALA
                     RSTDEPTTPNTPDE"
     gene            7811..8704
                     /locus_tag="ASC57_RS00320"
     CDS             7811..8704
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892474.1"
                     /locus_tag="ASC57_RS00320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_055503715.1"
                     /transl_table=11
                     /translation="MIERDKPWRGIMVATALPMREDLSVDLDAYGEHCRWLVDNGCDGV
                     VPNGSLGEYQTLTEQERISVMETAVQAVGGQNVMAGAGAYGSGESRRWAERARDAGCGS
                     ILLLPPNAYRADERAVIEHYREVAKAGVPIVAYNNPYDTKVDLTPALLAKLYEEGLVVG
                     VKEFTGDPRRAYEIAELAPGLDVLIGSDDALLELALAGAVGWIAGYPNALPVSNAALYH
                     AALAHDLETALPLYRKLHPLLRWDARTEFVQAIKLSMEIVGRPAGPCRAPRLPLTDEMA
                     AAVRAATEKALAEGLR"
     gene            8701..9702
                     /locus_tag="ASC57_RS00325"
     CDS             8701..9702
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="ASC57_RS00325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_055500940.1"
                     /transl_table=11
                     /translation="MRTRRVFHAVDSHTEGMPTRVITGGIGVIPGATMAERRVHFRDNL
                     DHIRTLLMTEPRGHSAMSGAILQPPTRPDADWGVLFIEVSGLLPMCGHGTIGVATVLVE
                     TGMVEVVEPVTTVRLEVPAGLVEAQVAVQDGMAMSVTLRNVPSFCDRLDASVTVPGFGE
                     IPYDLAYGGNFYAIVDLDRYGLPFDRKAKNEIIAAGLATMEAINAADEPVHPEDDRIRG
                     CHHVYFAAPGSDARHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLGTDFVNES
                     FIGTRFIGRLLEETQVGGRTAVVPSITGRAWITGTAQYFLDPRDPFPAGFEL"
     gene            9699..10682
                     /locus_tag="ASC57_RS00330"
     CDS             9699..10682
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544792.1"
                     /locus_tag="ASC57_RS00330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_055500941.1"
                     /transl_table=11
                     /translation="MITTVDYHTAGEPFRIVTGGVPDVPGATVLERRALLGAGGAAAED
                     VDRVRRLLCHEPRGHADMYGCFLVPPDDPGAHLGVLFWHKDGYSTACGHGTIALGVHAV
                     REGLVRADPDGETDVVVDVPSGRVTARVRCSGGAVRSVTFVSVPSYVIARGVRAGGVEV
                     DLAWGGAIYASVPAGALGLSVEPRHLTALIARARAIKADLAAHPAARHPSDERLSGVYG
                     VIFHDALPDADGVTRQRNVTVFADGEVDRSPCGSGTAARLALLHDEGLRGPLVHESVVG
                     SVFLARVAGVTEEGIVPEIEGMAYRTGRHVFELDPEDPIGTGFTLR"
     gene            10679..11617
                     /locus_tag="ASC57_RS00335"
     CDS             10679..11617
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544793.1"
                     /locus_tag="ASC57_RS00335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="WP_055500942.1"
                     /transl_table=11
                     /translation="MSALPYLGAAEIERLVPFGRAVDVLEEALRAGLDPEDTPRRPIVD
                     VPAGQLMLMPATAGRHAGVKAVTIAPGNPERGLPRIQGTYLLFDGETLAPLAAMDGIAL
                     TSLRTPAVSALAVRHLAEPDARHLVVFGSGPQAWGHVLALREVRPVERVTVVARDAGRA
                     EELAGRCRDLGLAAAARPAGEAAWEAVASADLVACCTTAREPLFPGKLARDGVTVVAVG
                     SHEPEARELDDDLVARATVVVEARSAALAEAGDVIIPIRHGTITSGHLAGNLADLIAGR
                     VTAGAGPRVFKSTGMAWEDLVVAAAAYEAWT"
     gene            11633..12301
                     /locus_tag="ASC57_RS00340"
     CDS             11633..12301
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544794.1"
                     /locus_tag="ASC57_RS00340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_055500943.1"
                     /transl_table=11
                     /translation="MAAEDRLDLPMVGERQSLREQVAHALRAALIAGEMRPGVVYSAPV
                     LAAQFGVSATPVREAMLDLAKEGLVEAVRNKGFRVTELSDRDLDELTEIRQLIEVPTVA
                     RMADSSRAEEFERLRPVAEEIVAAAERGDLLAYVDADLRFHVELLALSGNAHLVTVVRD
                     LRNRARLYGLSQLAARGALGESSREHLALLDALKRGDAEAVQRIMGEHIGHVRGIWAEH
                     "
     misc_feature    complement(12364..12377)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 17.46"
                     /tool="antismash"
     misc_feature    12503..12521
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.46"
                     /tool="antismash"
ORIGIN
        1 ctgtggctga cgacgaacct gctggccgcg ctgctgtcca ccgccgcgat cctcttctac
       61 gtcctcgtct actcgatgct gctcaagcgg cgtaccccgc agaacatcgt ctggggcggc
      121 atcgcgggct gcatgccggt cctgatcggc tgggcgggca tcaccgaccg gctcgactgg
      181 gcgccgctgg tgctgttcgg cgtggtgttc ttctggacgc cgccgcacac ctggaccctc
      241 gccatgcgtt acaaggagga ctacgcggcg gccgaggtgc cgatgctgcc ggtggtggcc
      301 accgagcgcc gcgtgatcat ggagtcgatc gcctacacct gggcgacggt gctgtgctcg
      361 ctgctgctgt ggccggtcgc gcacaccacg ctgttctacc cggcggtcgc cggggtgctg
      421 ggcgtgctgt gcctgtggga ggtccaccgc ctgctggcca ggctcaacgc cggcaagacg
      481 ggcgtggcgc tgcgcccgat gcgcttcttc cactggtcca acgcctatct ggcgctgctc
      541 ttcctcgccg tcgcgatcga cccgctgctc gcctgacccg tacgggggcg ttgcggcggg
      601 tcctcgcata ctcttatagt gcacatatga atagctcttc aggtgttgta gccgcgctgg
      661 acggctgcgt ggtgcaggaa cccgacgccg acagggtcgc cgccacccgc gaccgcctgg
      721 tgacgcccga ggaggccacc cggctggccg agctgttccg cctgctcggc gaccccaccc
      781 gggcccgcct gctgtacgcc ctgctggagg cgggcgagct gtgcgtgtgc gacctggccg
      841 acgcggtcga ggtcaccgac acggccgtct cccacgccct gcgcctgctg cgcaccgcgg
      901 gcatcgtggc cagccgcagg gcaggccgga tgatctacta ccggctcgcc gacgcccacg
      961 tgcgcatgct gctcgacctc agccgcgaac acctcaagga gaccggatga gcgccggaca
     1021 cgggcacggg cacgggcacg ggcacggggt gagcgcgaac gccgaccgtc gctacctggc
     1081 cggcgcgctc gcgctcatcc tcgcctacat ggcggccgag gtggtcgcgg gcatcgccgc
     1141 caactccctg gcgctgatct ccgacgccgc ccacatgctc accgacgcca gcgcgatcgc
     1201 cctggcgctg atcgccatgc gcatcgccgc caggccggcg cgtggcgcgt acacgttcgg
     1261 gtggaagcgc gccgagatcc tctccgcgca gatcaacggc ctgacgctgc tcctgctggt
     1321 cgcgtacttc gtctacgaga gcgtgcgcag gctcgccgag ccgcccgagg tgcaggggcc
     1381 cgtggtcgtg gtcacggcgg tggcgggcat cgtggtcaac gcggccgccg cctggctgct
     1441 gtcgcgggcc gaccggcgca gcctcaacgt cgagggcgcc ttccagcacg tgctcaacga
     1501 catgtacgcc ttcatcgcca ccgccgtctc cggcgtggtg gtctggctga ccggcttcac
     1561 ccgggccgac gcgatcgccg cgctcgtcgt ggccgccctc atgctgaagg cgggctgggc
     1621 gctgctgcgc gactcggccc gggtgctgct gcaggccgcg cccagcgacc tcgaccccga
     1681 cgagatcggc cgggtcctgg ccgcggaccc ggacgtcgtg gaggtgcacg acctgcacgt
     1741 ctggacggtg acgagcggct accccaccct gtcggcgcac gtcatcgtgg cgcgcggctc
     1801 ggactgccac cgggtgcgca cccggctggc cgacctgctg cacgagcggt tcgccatcgg
     1861 ccacacgacg ctccaggtcg accacggcga gcccgaccgc gagcggcact gcgtcgatcc
     1921 gcacggcccc cgccacctgg gaaggacacc gggcacctga tccgttaaag tcaccgtatg
     1981 gcgacccgtg atccacgctg ggtgttcaag gaccgcccgt cgttcgcgtt gatcaccggg
     2041 ctggtcgtca ccggcatctg cgctctcgtc tcgttctcgt tcgacgtgct caacggcgag
     2101 ccggtgcagt tcttcatcgc gctggtgctg gcgctcgcgc cggtgccgct gctcctcgcg
     2161 gccgtgctcg cgctcgaccg catggagccc gagccgcgca gcaacctcgc cttcgccttc
     2221 gcctggggcg ccggcatcgc cgtgctggtc gcgggcgtcg tcaactcgct caacctgcac
     2281 tacatcatcg acacggccaa gctctccgag gccagcgccc gcaacatcgc cgccacgttc
     2341 ggcgcgcccg tcgtcgagga gacgatgaag gggctggtgc tgctcggcct gctgcgcttc
     2401 cgccgcgcgg agctggacgg ccccaccgac ggcatcatct acgccagcat ggtggggctg
     2461 ggcttcgcca tgagcgagaa cgtcagctac tacatcgccg cgctcaactc cgccaacggc
     2521 tccggcgtca gggacctcgc ggtgaccgtg gtgctgcgcg gcatcctgtc gccgttcgcg
     2581 cacccgctgt tcagctcgat gatcggggtg ggcgtggcgt acgcggccct gcgcaacggg
     2641 cccgaccggg tgttcgtcgt gctggccggg tggtcgggcg ccatggtgct gcacggcctg
     2701 tggaacggtc tcgcgtcgta cggcgggctg cccggcctgg tcaccgcgta cctgacgctg
     2761 ctggtggtgc tggtcgtgct catcggcgtg gtgttcaggg accggcggcg catcgtggcg
     2821 ctcatccagc gctaccttcc gccgtacgag ccgaccaagc tggtgggcga ggccgacatc
     2881 cacatgctct cgacgatctc ccgcaggcgg cgggccaggc agtgggccaa ggcccacggc
     2941 ggcaagcagg ggctgcgcgc catgcgcgac taccagctcg ccgccaccga gctgggcctg
     3001 ctgcacgagc gcgcggcccg ccacgtggtg gacgagcaga ccttccacga ggagcagcgg
     3061 gcgctgctgg agtgcatgag cgcggcccgc gccggcttcc cgccccccgt gcccgtgcgg
     3121 ccgaccagga cgaggaggag gacgccgccg cccggctacg cgcccggttg agcgccggga
     3181 ggcggcccgg gccgcgagag gatcgtcgtc agcgacacct cgcggggccc cgggtagacg
     3241 gggtcggagt gcagcacgcg cgcgtcggcg aacgccttgg cggtggcgag cagctcgcgc
     3301 gcggcgtaca cgtacgtcgg gccggcgaac gcccggctgg agtcgtcgcc cgcgccgaac
     3361 gcctcgagcc ccgccgtgcg gcacagcgcg acggccctcg gcaggtggaa gacctgcgtg
     3421 acgacggtga cgcgctcggc gccgaacacc ctgcgcgccc tgacgcagga gtcccaggtg
     3481 tcgaagccgg cgtagtcgag caccatggcg gcgtcgggca cgcccttggc ccgcaggtag
     3541 tcgcgcatcg cgccgggctc gtcgtacgcc gtggtgctgt tgtcgcccga cagcagcagc
     3601 gccctgaccc ggccggcccg gtagaggtcg gcggcgatgt cgagcctgcg cgccagcatg
     3661 gggctgggcc tgccggccgc gaggccggcg ccgagcacga gcgcggcctg cgccgggggc
     3721 gtgcgctcca gccagccggg ggcgccggcg gtgacgcggt ggccggagct ggtgagccag
     3781 gcccaggtca tgggcgccag ggcgagcacg ctgaggagca cgagcccctg gtacgcccgc
     3841 cgcagcgtcc tgcgatcagg gcggacatct cccagcaaat cgcacccctc tcgcggtggg
     3901 ggagtggcgg acttatcacc gcatagcctg gcataaaggc ctagacgggg gcgggcacct
     3961 gggcggggga ggggacgggc tgccgggtgc gcagcgaggt gacggtccgc agcgtggcga
     4021 tccagaccag ggtggagccg aagacgtgca ggagcacgag cgccgcgggg acggcgagga
     4081 agtactgggt gtagccgatg acgccctggg ccagctcgac cccgagcagc gccagggcgg
     4141 cccggcgggc ggcgcgcggc gcgtcggtga cgtgcagggc gagcagcagc gcgaacgtca
     4201 gccccaccac caggtagacg acgtcggcgt gcagccgcgc caccgcctcc atgtcgaagt
     4261 cgaagcgcga ggcccgcagg tcgccggagt gcgggccggt gccggtgacg acgacgccca
     4321 ggacgaggag caccgagacg gcgccgacca ggacgtggcc gagccgcctg atgtcgcggt
     4381 gggcgagccg gacgggcggg gcgtcgccct cgccgctgcg cgcgtacagc atccagcagg
     4441 ccgcgaccag gccgatggag atcaggaagt gcatgcccac ggtgaagggg ttgagcgccg
     4501 tccgcaccac gagcccgccc cacagggcct gcgccaggac gccggcgggc tggagccagg
     4561 ccaggcgcac gagcgagacg cggcgcggcg ccatgcgcat ggccgagacc acgcacgcga
     4621 ccccgacggc gagcacgagg aacgtcagca gccggttgcc gaactccacc gccatgttga
     4681 gcatcgacgt ctcggggtgg gcgacgggga cgaagctgtc gggggtgcac cgcggccagg
     4741 tggggcagcc gaggccggag ccggtgacgc gcacgcccgc gccggtgacg gcgatgccgg
     4801 cgttcaccac cacggaggcc agggcccagc cccgcatgga ctcggcggtg ggcgtccaga
     4861 acgacagcag gctccggacg acggggttgg ggtggtccgc gggtaccttc acgtcatcga
     4921 tggtacgggc gggaccggcc ggtccttcca cgggcttctc acctcgccgg atatgtgaca
     4981 tattactggg ggaggtgaga atgtccgacg tcatcgtggt cggcgccggc atggtgggcg
     5041 ccgcgtgcgc ctactacgcg gcccgtgccg ggctcgacgt gaccgtcgtg gaccgcggcc
     5101 ccgtggccgg cggcacgacc ggggccggcg agggcaacgt gctggtgtcc gacaaggagc
     5161 ccggccccga gctggagctc gcgctgctgt ccaaccggct ctggcactcg ctggccgagg
     5221 ccggcgactt cgagttcgag ccgaagggcg gcctggtcgt cgccgagacc gaggaggtcc
     5281 agcgggcact cgtcgggctg gccggcaaac agggcgtgga gcacgccgtg gtggcggcgt
     5341 ccgagctgcg cgacctcgaa ccgcacctgg ccgaggggct cgccggcggc gtgctctacc
     5401 cgcaggacgc ccaggtgcag cccatgctgg ccgccgccag gctgctggag ctcggcgcgc
     5461 gcagctacgg ccacggcgcg ctgcgcctgc gcctcggcgt caccgtcacc gggttcgtca
     5521 ggtcgggcga ccgggtgacc ggcgtgcgca cctcgcaggg cgacatcctc ggcggcgccg
     5581 tcgtcaacgc ctccggcacc tggggcggcg aggtggccgc cctcgcgggc gtgcacctgc
     5641 cgatcctgcc caggcgcggg ttcatcctcg tcaccgagcc gctgccgccc atgatcaggc
     5701 acaaggtcta cacggccgcc tacgtcacca acgtggccag cgactcggcc gggctggaga
     5761 cgtccgccgt ggtcgagagc acccaggcgg gcaccgtcct catcggcgcc agcagggagc
     5821 gggtcggctt cgaccgcacc acctcggtgc cggtgctcgc gcgcctggcc gcccaggccg
     5881 tcgcgctctt ccccgcgctg cgcgacgtgc gggccatcag gtcgtaccac ggcttccgcc
     5941 cgtactgccc cgaccacctg ccggtgatcg gccccgaccc gcgcgcgccc ggcctctacc
     6001 acgcctgcgg ccacgagggc gcgggcatcg gcctcgcccc cgccaccggg cacctgatgg
     6061 cccagctcct caccggcgcc gcccccgacc tcgacccgca accgttccgc ccggaccgct
     6121 tcccggagga gccgcagtga ccgaaccctt caccatcacc gtggacggcc gccaggtggc
     6181 cgtcacgccg gggcagacga tcggcgcggc cctgcacgcg gccgggatcc gctcctggcg
     6241 ctccacccgc ttctgcgggc gcccgcgcgg cctgttctgc ggcatcggcg tgtgcttcga
     6301 ctgcctggtc agcgtcaacg ggcggccggc agagcgggcc tgcctgcgcg aggcagcccc
     6361 cggggacgag gtgaccacct catgagcggc gtccgccacg acctcgtcgt cgtcggaggg
     6421 ggacccgccg gcgtcgccgg cgcgctcacc gcggccctgg ccgggctgcg cgtggcgctg
     6481 gtcgactcct cggcccggct cggcggccag tacttccgcc actcgcgggc ggcgcaggac
     6541 gcgcccggcg gcgcgcccga gctggacggc ttcctgcggc gcggccgcgc cctcgccgag
     6601 cgcgccgacc tgctcctcgg ccaccgcgtc tggcacgtct cccgcgagga cggcggcctc
     6661 gccgtgcact gcgtgacccc ggacgagcag ccgctgaccg tgcgcggcgc ccgcctgctc
     6721 atcgccaccg gcgcccacga ccgcgtcgtg cccttccccg gctgggacct gcccggcgtg
     6781 ctcacagcgg gcggcgccca ggccctgctc aagggcagcg gcgtcctcgc cggccggcgc
     6841 gccgtcgtgg ccggcaccgg gccgttcctg ctgcccgtcg ccgccgggct cgccggggcg
     6901 ggcgccgacg tcctcgccgt gtacgaggcc gcccgcggtc tcggcctggc caggcatccc
     6961 gtgctcgccc tcggcaaggc cggcgaggcc gccgggtacg ccctggccct ggccaggcac
     7021 cgggtgccgt acctgaccag gcgggcggtc gtcgccgccc acggcgacgg cgcgctggag
     7081 gcggtgaccg tcgccaggat cgacgccggc tggaacgtcg tggacacccg cgtcgtcgag
     7141 tgcgacaccc tggccgtcgg ccacggcttc gtcccgcaga tcgagctggg cgtggagctg
     7201 ggctgcggca cccgcgccga cacggacggc agccccgtcc tcgtggtgga cgccgcccag
     7261 cgcaccaccg tccccggcgt gtgggccgcg ggcgagccca ccggcgtcgg cggctggcgg
     7321 ctggccgagg tggagggccg cgtcgccggc cacgccgtcg ccgccgagca ccgtgcctcg
     7381 tcccgtgcct cgaccggagc gcccgcggtc tccgggggcg ggcacggcgc cccgcgcgcc
     7441 ctgctcgccc ggcggcgcgg gatgcgcgcc ttcgccgagg cgctccagcg cgcctacccc
     7501 gtcgcccccg gctggcaggg ctggctgcgc gacgacacgc tcgtgtgccg gtgcgaggag
     7561 gtgccggtgt cacgcgtacg cgaggcgcag cggctcggcg ccaccgacgc gcgctcgatc
     7621 aagctgctgg ccaggcccgg catgggctgg tgccaggggc ggatctgcgg ctacgccgtc
     7681 tcctgcctgg ccggggagga gccccggccg cccaagcggc ccatcgcgca gccgatcacg
     7741 ctcggcgcgc tggcccgctc caccgacgaa ccgacgaccc ccaacacccc ggatgaatga
     7801 ggagcacgtg atgatcgaac gcgacaagcc ctggcgcggc atcatggtgg ccaccgcgct
     7861 gcccatgcgc gaggacctgt cggttgacct ggacgcctac ggcgagcact gccgctggct
     7921 ggtggacaac gggtgcgacg gcgtggtgcc gaacggctcg ctcggcgagt accagacgct
     7981 gaccgagcag gagcggatct ccgtcatgga gaccgcggtg caggccgtcg gcggccagaa
     8041 cgtgatggcc ggcgccggcg cgtacggctc gggcgagtcg cgccgctggg ccgagcgggc
     8101 ccgcgacgcg ggctgcggct cgatcctcct gctgccgccg aacgcctacc gggccgacga
     8161 gcgggccgtg atcgagcact accgggaggt ggccaaggcc ggcgtgccga tcgtcgccta
     8221 caacaacccg tacgacacca aggtggacct caccccggcg ctgctcgcca agctgtacga
     8281 ggagggcctg gtcgtcggcg tcaaggagtt caccggcgac ccgcggcggg cgtacgagat
     8341 cgccgagctg gcccccggcc tcgacgtgct gatcggctcc gacgacgcgc tgctggagct
     8401 ggcactggcc ggcgccgtcg gctggatcgc cggctacccg aacgcgcttc ccgtctccaa
     8461 cgccgcgctc taccacgccg cgctggccca cgacctggag accgcgctgc cgctgtacag
     8521 gaagctgcac ccgctgctgc gctgggacgc caggaccgag ttcgtccagg cgatcaagct
     8581 gtcgatggag atcgtcggcc gtcccgccgg cccctgccgc gcgcctaggc tgccgctgac
     8641 cgacgagatg gcggccgcgg tgcgcgccgc cacggagaag gcgctcgcgg agggcctgcg
     8701 atgaggaccc ggcgggtctt ccacgccgtc gactcgcaca cggagggcat gccgacccgc
     8761 gtcatcacgg gcgggatcgg cgtgatcccc ggcgcgacga tggccgagcg gcgcgtccac
     8821 ttccgcgaca acctcgacca catccgcacg ctgctgatga cggagccgcg cggccactcc
     8881 gccatgagcg gcgccatcct ccagcccccg acccggcccg acgccgactg gggcgtgctg
     8941 ttcatcgagg tgtcggggct cctgccgatg tgcggccacg ggacgatcgg cgtggccacc
     9001 gtcctcgtcg agaccggcat ggtcgaggtg gtcgagccgg tcaccaccgt gcggctggag
     9061 gtgcccgccg ggctggtcga ggcgcaggtc gccgtccagg acggcatggc catgtcggtg
     9121 acgctgcgca acgtgccgtc gttctgcgac aggctcgacg cgagcgtcac cgtgcccggc
     9181 ttcggggaga tcccgtacga cctggcctac ggcggcaact tctacgccat cgtcgacctc
     9241 gacaggtacg gcctgccgtt cgaccgcaag gccaagaacg agatcatcgc ggcgggcctg
     9301 gcgacgatgg aggcgatcaa cgccgcggac gagcccgtgc accccgagga cgaccgcatc
     9361 cgcggctgcc accacgtcta cttcgccgcg ccgggctccg acgcccgcca ctcgcggcac
     9421 gccatggcga tccacccggg ctggttcgac cggtcgccct gcggcaccgg cacgagcgcc
     9481 cgcatggcgc agctccacgc ccgcggcgag ctgccgctcg gcacggactt cgtcaacgag
     9541 tcgttcatcg gcaccaggtt catcggcagg ctcctggagg agacacaggt cggaggcagg
     9601 acggcggtcg tgccgtcgat caccgggcgg gcctggatca ccggtacggc gcagtacttc
     9661 ctcgacccgc gcgacccgtt ccccgcgggg ttcgagctgt gatcaccacc gtcgactacc
     9721 acacggccgg cgagccgttc aggatcgtga ccgggggcgt ccccgacgtc cccggcgcga
     9781 ccgtgctgga gcggcgcgcc ctcctcggcg ccggcggcgc cgccgccgag gacgtggacc
     9841 gggtgcgccg cctgctctgc cacgagccgc gcggccacgc cgacatgtac ggctgcttcc
     9901 tcgtgccgcc cgacgaccct ggcgcgcacc tgggcgtgct gttctggcac aaggacggct
     9961 actccacggc ctgcggccac ggcacgatcg cgctcggcgt ccacgccgtg cgcgagggcc
    10021 tggtgcgggc cgaccccgac ggcgagacgg acgtggtcgt ggacgtgccg tccggccggg
    10081 tcaccgcccg cgtgcgctgc tcgggcggcg cggtcaggtc ggtgacgttc gtcagcgtgc
    10141 cgtcgtacgt gatcgcgcgc ggcgtccggg cgggcggcgt cgaggtggac ctggcctggg
    10201 gcggggcgat ctacgccagc gtcccggccg gcgcgctcgg gctgtcggtc gagccgcgcc
    10261 acctgaccgc gctcatcgcc cgcgcccgcg cgatcaaggc ggacctggcc gcccacccgg
    10321 ccgcccgcca cccgtccgac gagcggctgt ccggcgtgta cggcgtgatc ttccacgacg
    10381 cgctgccgga cgcggacggc gtcacccgcc agcgcaacgt caccgtgttc gccgacggcg
    10441 aggtggaccg ctcgccgtgc ggctcgggca cggcggcacg gctggccctg ctgcacgacg
    10501 aggggctgcg cggcccgctc gtccacgaga gcgtcgtcgg cagcgtcttc ctggcgcgcg
    10561 tggccggcgt gaccgaggag gggatcgtgc ctgagatcga gggcatggcg taccgcaccg
    10621 gcaggcacgt cttcgagctc gaccctgagg acccgatcgg gacggggttc acgctgcgat
    10681 gagcgccctt ccctacctcg gcgccgccga gatcgagcgc ctggtgccgt tcggccgggc
    10741 cgtggacgtg ctggaggagg cgctgcgggc cgggctcgac cccgaggaca ccccgcggcg
    10801 gcccatcgtg gacgtcccgg ccgggcagct catgctgatg ccggccacgg cgggccgcca
    10861 cgcgggcgtc aaggcggtca cgatcgcgcc cggcaacccg gagcgggggc tgccccgcat
    10921 ccaggggacg tacctgctgt tcgacggcga gacgctggcg ccgctcgccg ccatggacgg
    10981 catcgcgctc acctcgctgc gcacgcccgc cgtgtcggcg ctcgccgtgc ggcacctggc
    11041 cgagccggac gcccgccacc tggtggtctt cggcagcggc ccgcaggcgt ggggccacgt
    11101 gctggccttg cgcgaggtgc ggcccgtcga gcgcgtcacc gtggtcgcgc gcgacgccgg
    11161 gcgggcggag gagctcgccg ggcgctgccg ggacctgggt ctcgcggcgg cggcgaggcc
    11221 cgcgggcgag gcggcctggg aggcggtggc gtcggccgat ctggtcgcct gctgcacgac
    11281 cgcccgcgag ccgctgttcc ccggtaagct cgcccgcgac ggcgtcacgg tcgtcgcggt
    11341 cggctcccac gagccggagg cccgcgagct cgacgacgac ctcgtcgccc gcgccacggt
    11401 ggtcgtcgag gccaggtccg cggcgctggc cgaggccggc gatgtgatca tcccgatccg
    11461 ccacggtacg atcacctctg gtcatctcgc cgggaacctc gcggacctga tagccggccg
    11521 ggtgaccgcc ggcgccgggc cgcgcgtgtt caagagcacg ggcatggcct gggaggacct
    11581 ggtggtcgct gccgccgcct acgaggcgtg gacctagcga agggatgggc cgatggctgc
    11641 cgaggaccgg ctcgacctgc cgatggtggg cgagcggcag agcctgcgcg agcaggtggc
    11701 gcacgcgctg cgcgccgcgc tgatcgcggg cgagatgcga ccgggcgtgg tctactccgc
    11761 gcccgtcctc gccgcgcagt tcggcgtctc ggccacgccc gtccgcgagg ccatgctcga
    11821 cctcgccaag gagggcctgg tcgaggcggt gcgcaacaag ggcttcaggg tgaccgagct
    11881 gtccgaccgc gacctcgacg agctcacgga gatccgccag ctcatcgagg tgcccacggt
    11941 cgcgcgcatg gccgacagct cgcgggcgga ggagttcgag cggctgaggc cggtggccga
    12001 ggagatcgtg gccgccgccg agcggggcga cctgctcgcg tacgtcgacg ccgacctgcg
    12061 cttccacgtc gagctgctgg cgctgtcggg caacgcccac ctggtcacgg tggtgcgcga
    12121 cctgcgcaac agggccaggc tgtacggcct gtcgcagctc gcggcgcggg gcgcgctggg
    12181 ggagtcgtcc agggagcatc tcgcgctgct cgacgcgctc aagcgcggcg acgcggaggc
    12241 ggtccagcgc atcatgggcg agcacatcgg gcacgtcagg ggcatctggg ccgagcactg
    12301 aacctgtccg ttatgcggag ttttgtctga tttgaggtac cctggcggta agtcaggcct
    12361 ggtcaagcgg acgttcgcgc tccgctgacc ttcgcgggct cggcgccccg cccaccgaac
    12421 agccgagccc tccgccgcgc gcccgaaggc cccatccccc gggcgcgcgg cgggccccag
    12481 ggggcgccgc cggagggcgg gctcaggtcc ggctcgccgt cctcgtcgag cccaggctgg
    12541 ccgccacgat gcaggcgatg gccacccact gcacgacatg cagcacctcg cccagcagca
    12601 gcacgccgat cagcgccgcc acgcccggct cgacgctcat gaggatgccg aagacgtgct
    12661 tgggcatccg ccgcagcgcc tgcagctcca gcgagtacgg gatcaccgac gacagcagcc
    12721 ccacgccgag gccgatgagc agcaggtgcg gctggagcag gtcggcgccg ccggtgacga
    12781 cgcccacggg cgcgatcgcc
//
