LOCUS       NZ_BBOK01000019        12988 bp    DNA     linear   CON 17-AUG-2024
DEFINITION  Streptomyces sp. NBRC 110030, whole genome shotgun sequence.
ACCESSION   NZ_BBOK01000019
VERSION     NZ_BBOK01000019.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. NBRC 110030
  ORGANISM  Streptomyces sp. NBRC 110030
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Katano-Makiyama,Y., Hosoyama,A., Hashimoto,M., Tsuchikane,K.,
            Uohara,A., Kitahashi,Y., Ohji,S., Ichikawa,N., Kimura,A.,
            Yamazoe,A., Komaki,H., Igarashi,Y. and Fujita,N.
  TITLE     Whole genome shotgun sequence of Streptomyces sp. NBRC 110030
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 96168)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 97x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417675.1-RS_2024_08_17
            Annotation Date                   :: 08/17/2024 02:44:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,152
            Genes (coding)                    :: 7,019
            CDSs (with protein)               :: 7,019
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 45 of 133
            Pseudo Genes (incomplete)         :: 107 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 23 of 133
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:33:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 45583
            Orig. end                         :: 58571
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBOK01000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12988
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="2"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12988
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12988
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7988](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7988)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(391..2262)
                     /locus_tag="APK71_RS34405"
     CDS             complement(391..2262)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445572.1"
                     /locus_tag="APK71_RS34405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_055469949.1"
                     /transl_table=11
                     /translation="MGTTESRGPAPGRSAVVLALRHYGLELLRLRRLALPALLLPAVGN
                     IGIRYVAPLLIAKLAGQAAGDGLALGPALPYVLGFGATLLLAEAVWRIGQHCLNRVCAL
                     GMEHLYVSGMDELLAKDAAFFHDNFAGSLTKRVLSYGKRFEDFTDTLTYRIVGSLVPLL
                     FGAVVLWSYEPMLVVGLFVMLVLTATAATPLIRRRQRLVNDREAAIARVSGHVADSLAN
                     METIRAFAAEGREGAEHRSRVAESRRLTLRSWDYGNLRVDTLIAPLSVLTNVLGLVVVL
                     ALGGPGQGVEEVVVAFTYYSNATQIMFEFNQIYRRLESSMTEAAQFTELLLDPPTVLDA
                     VDPEPLAPRDTGVRFEAVTFAHAGAEPIFRGLDLDVPAGARFGLVGRSGGGKTTLTRLL
                     LRMSDLDRGRILIGGQDISRLRQSDLRSLIAYVPQEPAMFHRSLRDNIAFARPGATEDE
                     IRAAAAAAHVTEFADQLPEGFATLVGERGVKLSGGQRQRVALARAILRDAPILLLDEAT
                     SALDSESEILVQDALWRLMDGRTALVVAHRLSTVAGMDRLVVLDRGRVVEQGSHDKLLA
                     TDGAYARLWQHQSGGFLGEAAEPALEHPVPGPGPGDPHGPADPVPVV"
     misc_feature    complement(2251..2266)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2361..2375)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.68"
                     /tool="antismash"
     misc_feature    complement(2381..2397)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(2383..2393)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    2383..2395
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            2434..3114
                     /locus_tag="APK71_RS34410"
     CDS             2434..3114
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267155.1"
                     /locus_tag="APK71_RS34410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_031084418.1"
                     /transl_table=11
                     /translation="MPHLKSQAIPVQAPLRDQVAEALRAALIAGDLRPGVVYSAPTLAA
                     ELGVSATPVREAMLDLAREGLVEAVRNKGFRVTEMTERDLDEFTEIRTLIEVPTVGRVA
                     TTATAEQLEALRPSAQEIVTAAREHDVLAYVEADHRFHLGLLSLAGNHHLVQVVADLRT
                     RSRLFGLGSLNETGRLVASAEEHVELLDLMVAGRGAEAEECMRRHLAHVRTLWAEGTAR
                     PLRG"
     gene            complement(3104..4105)
                     /locus_tag="APK71_RS34415"
     CDS             complement(3104..4105)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="APK71_RS34415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_030403485.1"
                     /transl_table=11
                     /translation="MRSTVCYHAVDSHTEGMPTRVITGGVGVLPGATMSEKRQRFVADR
                     DDLRTLLMCEPRGHASMSGAILQPPTRPDADYGVLFIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEVTEPETRVRLDTPAGLVTARVRVRDGHAESVTLENVASYCHALDQVADVPGYGQ
                     VRYDIAYGGNFYAFVRTDDLGIPFGRAHKQRLLDAGLAVMDAVNEQNPVSHPENPEIDV
                     CHHVYLEAPGSTAAHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGLLPAGRDFVNES
                     FIGSRFVGRILGETTVGGRPAVLPSVTGRAWITGTAQYLLDPTDPYPAGFTL"
     gene            complement(4108..5004)
                     /locus_tag="APK71_RS34420"
     CDS             complement(4108..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030799.1"
                     /locus_tag="APK71_RS34420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_030403484.1"
                     /transl_table=11
                     /translation="MSETRKPWHGVIVATGLPFHDDLSVDFGAYGESVAHLAEQGMHGV
                     APNGSLGEYQTLTYEERDRVVETAVAHAPEGFTVMPGVGAYGGREAKRHAQFAKDAGCQ
                     AVMCLPPNAYRADDRAVLEHFELVASAGLPVTAYNNPVDTKVDLRPDLLAKLYAEGFVV
                     GVKEFSGDVRRCYEIAELAPGLDLMIGTDDTVLEVALAGAKGWVAGYPQVFPRACLALY
                     EASVRGDLETALPLYRQLHPVLRWDSKTEFVQAIKLGQELTGRRGGPCRPPRQPLAPET
                     EAVVRAATQALIDAGVN"
     gene            complement(5001..6458)
                     /locus_tag="APK71_RS34425"
     CDS             complement(5001..6458)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059558.1"
                     /locus_tag="APK71_RS34425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_055469950.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.8e-19, bitscore:
                     62.4, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 5.8e-13, bitscore:
                     41.5, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEPVDLVVVGAGPAGMTAAATALAGGLRVVLLDSGTALGGQYWR
                     HPPEHARAAVPTDDLHHGLGRYRALCRALAAHRSAGRLDLRLAHHAWSAVREGDGFAVH
                     VVDRRDAPRETAAVLRAPRLLVATGAYDRQLPFPGWDLPGVLTAGGLQALLKGGGVAAG
                     SRVALGGTGPFLLPVAAGLAARGVEVVAVCEAAHPRAWLRHPGPLLRNPGKWAEGASYA
                     ATLARHRVPVRPRTAIVAAEGGARVTAVRLAALASNGSPRPGTERRVEVDAVGVGWGFA
                     PQLDLLVPLGCALTESADGNAVAAVDAGQRTTVPGLYTAGETCGVGGAALAAHEGRLAA
                     WSVLADSGAAAPAPANRARTAVRSAVARQRAFARAMALAHPVPRAWPAWLTDETVVCRC
                     EEVTAGAVRTARADGAHDHRQVKQLTRAGMGWCQGRMCGPAVHCLTGRGRAYEPAERLV
                     ATPVTLGALAGLDEEPTDNAEPDKECS"
     gene            complement(6451..6705)
                     /locus_tag="APK71_RS34430"
     CDS             complement(6451..6705)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972635.1"
                     /locus_tag="APK71_RS34430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_030403482.1"
                     /sec_met_domain="Fer2_4 (E-value: 3.2e-25, bitscore: 80.5,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEIVVDGDPLPFVEGQTVAAALVAAGRVAWRTTRVGRRPRGVFC
                     GIGVCFDCLVTVDGARGQRACLLPARPGMTVTTREDDDE"
     gene            complement(6702..7988)
                     /locus_tag="APK71_RS36960"
     CDS             complement(6702..7988)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /locus_tag="APK71_RS36960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_079080737.1"
                     /sec_met_domain="DAO (E-value: 1.2e-66, bitscore: 218.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEEPLTLKVVVVGAGVVGAACAFHAASAGLDVTVVDRGPVGAGTT
                     SRGEGNVLLSDKEPGPELALARLSRTLWDEAGRELGADTLELEPKGGLVVAGTDESLAA
                     LREFAARQEAAGVRAEPVDRVRDLEPHLAPGIPGGVHYPQDAQVQPVLAAAALLRAAVR
                     RGARFHTGEVVGALTTRDGDRTPLGPTPHGPTSHGPAPVGPAPVGPVPVGPVPGGRITG
                     VRTAAGEVLPADAVVNAAGTWGGEVGRRLGAPVEVLPRRGFVLVTEPLPPMVRHKVYSA
                     DYVANVASSDAGLETSCVVEGTRGGTILVGASRERVGFDTTLNTAVVARLAAQACRLFP
                     FLRGVHLMRAYRGFRPYCPDHLPVIGPDPRVPGVVHACGHEGAGIGLAPGTGALVTAHL
                     LGRPWRGADPAAHTGLLPDRFLTPGGAPQ"
     misc_feature    8005..8021
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 14.49"
                     /tool="antismash"
     misc_feature    8009..8019
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     gene            complement(8082..10430)
                     /locus_tag="APK71_RS39080"
     CDS             complement(8082..10430)
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030778.1"
                     /locus_tag="APK71_RS39080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SWIM zinc finger family protein"
                     /protein_id="WP_107094435.1"
                     /transl_table=11
                     /translation="MSPRPADEARRALREARERAGGPGGEHGPAAPAATSSEAPAEAAP
                     SAPSAPAHSHERAPDGRPAEPSGPRPGDTARAALRRTRHAGAHDPAGARDAPSHREGPA
                     GGAEPASPARPPGSADPGDSARRAEEGDRSDDATTPTAPTAPTAPDEASRSGAVEGGDS
                     RPADVAREALRAARRKAKADAASEVASAKDASAKGVAHPRRAPRPAGGRRRAGGAGTAD
                     ARARAVRDFVAGAFRLPSEADPAGQISSAGETGKTGESGEILPADERAGRAPRGVRASA
                     SPEPAPSSGVGPDSGTGTPPAASPPAGRTPRSEDPAPDPGRPERRRHPEQASAVRDRAA
                     AHPAPPPAPPPARPRAPRSMAAPDRDGELRRTFPALPPRAVADEGLAGTWWGNAWVTAL
                     EEGALDPARLERGRGYAERGRVDAITVTPGLVLAYVQGSRARPYRVQVRLRTLGDADWD
                     RFLDAAVERPGHIAALLDKELPHSLADLADHGVPLLPGPGDLAPQCSCPDSGHPCKHAA
                     ALCYQTARLLDADPFVLLLLRGRGERELLDALSRRNAARAARTAQDGGPAPLPGVRAGE
                     VLARRVLPPLPAPLPAPPHPEQPPACPTAPGGPDSFALDQLATDAAARAHALLTTGRDP
                     VGGLTLWQDAVRLAAARPGSGLTAGTRALYASLASAAGRDTAELARAVAAWRQGGPEGL
                     DVLEEPWDPPAGRFDRARPLLLAADLPAFRPWRNRLTHPRGHVQLRLGRTGLWYAYESE
                     PGREDWWPRGTPDLDPVGALTGLGTRVDL"
     misc_feature    10507..10521
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     misc_feature    11613..11628
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: medium, score: 24.97"
                     /tool="antismash"
     misc_feature    complement(11820..11833)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.47"
                     /tool="antismash"
ORIGIN
        1 gcgccgccgg tgagccgacg ctcgcgccct cgatcacggg gaccagctgc gagcgcgcga
       61 acgtggcggc cgagacgtcg ccgtaggtgt agccgagtcc ggtcctgccg ccggcgtgca
      121 cccggacgag gacgagggtg gtggcgtccc attccagggt gccgtcctgc tccttgccgt
      181 cggggccgtc ggtgggcacc tcgaaggcgt gcacgtcgac cgcgtcgacc gtgcacgcgt
      241 cgaggggatc ggcgtcctgg ggcatggggc tctctcctgg gcgggcgggg aaggacactc
      301 tcggagtgcc ttccccggcc gggggcaaac cggacgacgg tcactcgttc ggtcgtcccg
      361 cgctccgggc cgcccgcccc ggagcggccg tcacaccacc ggtacggggt cggccgggcc
      421 gtgcgggtcg ccggggccgg gccccgggac ggggtgttcg agggccggtt cggcggcctc
      481 gccgaggaag ccgcccgact ggtgctgcca cagccgggcg taggcgccgt ccgtcgcgag
      541 gagcttgtcg tggctgccct gctcgacgac gcgtccccgg tcgaggacga cgaggcggtc
      601 catgccggcg acggtgctca ggcggtgggc gaccacgagg gccgtgcgtc cgtccatgag
      661 ccgccacagg gcgtcctgga cgaggatctc gctctccgag tccagggcgc tggtcgcctc
      721 gtcgagcagc aggatcgggg cgtcgcgcag gatggcccgg gccagggcga cgcgctggcg
      781 ctggccgccc gagagcttca cgccccgctc ccccaccagc gtggcgaagc cctcggggag
      841 ctggtcggcg aactccgtga cgtgggcggc cgcggcggcc gcgcggatct cgtcctcggt
      901 ggcgccgggc cgggcgaagg cgatgttgtc ccgcaggctg cggtggaaca tcgcgggttc
      961 ctgcgggacg taggcgatca gtgagcgcag gtcgctctgg cgcagccggc tgatgtcctg
     1021 gccgccgatc aggatgcgtc cccggtcgag gtccgacatc cgcagcagca gccgggtgag
     1081 ggtggtcttg cccccgccgg acctgccgac caggccgaac cgggcgcccg ccgggacgtc
     1141 caggtcgagc ccgcggaaga tcggctcggc gcccgcgtgg gcgaaggtca ccgcctcgaa
     1201 gcggacgccg gtgtcccggg gcgcgagcgg ttcggggtcg acggcgtcca gcacggtggg
     1261 cgggtcgagg agcagctcgg tgaactgcgc ggcctcggtc atggagcttt ccagccgccg
     1321 gtagatctgg ttgaactcga acatgatctg ggtggcgttg gagtagtagg tgaaggcgac
     1381 gacgacctcc tccaccccct ggcccgggcc gccgagggcg aggacgacca ccaggcccag
     1441 cacgttggtc aggacggaca ggggggcgat cagggtgtcg acgcgcaggt tgccgtagtc
     1501 ccaggacctc agcgtcaggc gccgggactc cgcgacccgg ctgcggtgtt cggcgccctc
     1561 gcgcccttcg gccgcgaacg cccggatggt ctccatgttc gcgagactgt cggcgacgtg
     1621 gccggagacc cgggcgatcg ccgcctcccg gtcgttgacg agccgctgcc tgcgccggat
     1681 cagcggggtc gcggccgtcg cggtcagcac gagcatcacg aacaggccga cgaccagcat
     1741 cggttcgtag ctccacagca ccacggcccc gaagagcagg gggacgaggc tgcccacgat
     1801 gcggtacgtc aacgtgtcgg tgaagtcctc gaagcgcttg ccgtagctga gcacccgttt
     1861 ggtcagggag ccggcgaagt tgtcgtggaa gaaggcggcg tccttggcga ggagttcgtc
     1921 catgccgctc acatagaggt gttccatgcc gagggcgcac acccggttca ggcagtgctg
     1981 cccgatccgc cagacggcct cggcgagcag cagcgtcgca ccgaagccca gcacgtacgg
     2041 cagtgccggg ccgagggcga gaccgtcgcc ggccgcctgt ccggccaact tggcgatcag
     2101 cagcggggcg acgtagcgga tgccgatgtt gcccacggcc ggcagcagca gcgcgggcag
     2161 cgccaggcgg cgcagtcgca gcagttccag gccgtagtgg cgcagcgcca ggacgaccgc
     2221 gctcctgccg ggcgccggtc ctcgcgattc tgtggtcccc atcacactcc cggtggttcg
     2281 gaagccggaa gtctcccgtc caacgaggcg cacagtccag gcatttaacc cggaccgtcg
     2341 agaatcggac accccacgcc ggggccggac ggcctgcgca acgtgacatt gtaaagtggc
     2401 agcccccttg gccggccgga cgcaggagca cggatgccgc atctcaagtc ccaggcgatc
     2461 cccgtccagg cccctctgcg cgaccaggtc gccgaagcgc tccgtgccgc cctcatcgcc
     2521 ggtgacctgc gtccgggcgt cgtctactcc gcccccaccc tggccgccga actcggcgtc
     2581 tccgcgaccc cggtgcgcga ggccatgctg gacctggccc gggagggcct ggtcgaagcg
     2641 gtgcgcaaca agggcttcag ggtcaccgag atgaccgagc gggacctcga cgagttcacc
     2701 gagatccgca ccctgatcga ggtgcccacc gtgggacggg tcgcgacgac ggcgaccgcg
     2761 gagcagctgg aggccctgcg gccgtcggcg caggagatcg tcaccgcggc ccgggagcac
     2821 gacgtcctcg cgtacgtcga ggcggatcac cggttccacc tcgggctgct gtccctggcg
     2881 ggcaaccacc atctcgtgca ggtggtcgcc gacctgcgca cacgctcacg tctgttcggg
     2941 ctgggcagcc tgaacgagac gggccggctg gtcgcttccg ccgaggaaca cgtcgaactg
     3001 ctggatctga tggtggccgg gcgcggcgcc gaggccgagg agtgcatgcg ccgccatctc
     3061 gcgcacgtcc gcaccctctg ggcggagggc accgcccggc ccctcagagg gtgaatccgg
     3121 ccgggtaggg gtccgtgggg tcgagcaggt actgcgcggt gccggtgatc caggcccggc
     3181 cggtgacgga cggcaggacc gccggacggc ccccgaccgt cgtctcgccg aggatccggc
     3241 cgacgaaccg ggagccgatg aacgactcgt tgacgaagtc ccggccggcc ggcagcaggc
     3301 cgcgggcgtg caactgcgcc atccgggcgc tggtgcccgt gccgcagggc gaccggtcga
     3361 accagcccgg gtggatggcc atggcgtgcc gcgagtgtgc ggcggtcgag ccgggggctt
     3421 cgagatagac gtggtggcac acgtcgatct cgggattctc cgggtgggac accgggttct
     3481 gctcgttgac ggcgtccatg acggccagtc cggcgtcgag cagccgctgc ttgtgcgccc
     3541 gtccgaaggg gatgccgagg tcgtcggtgc ggacgaaggc gtagaagttg ccgccgtagg
     3601 cgatgtcgta gcgcacctgc ccgtatccgg ggacgtcggc cacctggtcg agcgcgtggc
     3661 agtacgacgc cacgttctcc agggtcaccg actcggcgtg gccgtcgcgc acccgtaccc
     3721 gggcggtgac cagtccggcc ggggtgtcga gccggacccg ggtctccggc tcggtcacct
     3781 cgaccatccc ggtctcgacg aggaccgtgg cgacaccgat ggtcccgtga ccgcacatcg
     3841 gcaggcagcc ggagacctcg atgaagagca ccccgtagtc ggcgtccgga cgggtcggcg
     3901 gctgcaggat cgccccggac atcgaggcgt gcccgcgtgg ttcgcacatc aggagggtgc
     3961 gcaggtcgtc gcggtccgcg acgaaccgct gccgtttctc ggacatcgtc gcgccgggga
     4021 ggacgcccac gccgcccgtg atcacgcggg tgggcatgcc ctcggtgtgc gagtcgacgg
     4081 cgtggtagca gaccgtcgag cgcatcgtca gttcaccccg gcgtcgatca gggcctgggt
     4141 ggcggcgcgc acgacggcct ccgtctccgg ggccagcggc tgccgcggcg gccggcacgg
     4201 cccgccccgg cggccggtca gctcctggcc gagcttgatg gcctggacga actccgtctt
     4261 gctgtcccac cgcaggaccg ggtgcagttg gcggtagagc ggcagcgcgg tctccaggtc
     4321 gccccggacg gacgcctcgt acagggcgag gcaggcccgg gggaagacct gcgggtatcc
     4381 ggcgacccag cccttggcgc cggccagggc gacctccagc acggtgtcgt cggtgccgat
     4441 catgaggtcg aggccggggg cgagttcggc gatctcgtag cagcgccgga cgtcgccgga
     4501 gaactccttg accccgacca cgaacccttc ggcgtacagc ttggccagca ggtcggggcg
     4561 caggtccacc ttggtgtcga cggggttgtt gtacgcggtg acgggcaggc ccgccgaggc
     4621 caccagttcg aagtgttcga gcaccgcgcg gtcgtcggcg cggtaggcgt tgggcggcag
     4681 gcacatgacc gcctggcagc ccgcgtcctt ggcgaactgc gcgtgccgct tggcctcccg
     4741 gccgccgtag gcgccgacgc ccggcatgac ggtgaacccc tcgggcgcgt gggcgacggc
     4801 ggtctcgacg acgcggtcgc gctcctcgta cgtgagggtc tggtactcgc ccagcgaccc
     4861 gttgggcgcg acgccgtgca tcccctgctc ggcgaggtgg gcgacgctct cgccgtaggc
     4921 gccgaagtcg accgagaggt cgtcgtggaa cggcaggccg gtcgcgacga tgacgccgtg
     4981 ccagggcttg cgggtctcgc tcatgaacac tccttgtcgg gttcagcgtt gtccgtcggt
     5041 tcttcgtcga ggccggcgag cgcaccgagg gtgacgggcg tcgcgacgag ccgttcggcg
     5101 ggctcgtagg cccgtccccg tcccgtgagg cagtgcaccg cgggaccgca catccgcccc
     5161 tggcaccagc ccatgccggc ccgggtcagt tgcttgacct gccggtggtc gtgggcgccg
     5221 tcggcgcggg cggtccgcac cgccccggcg gtcacctcct cgcagcggca cacgacggtc
     5281 tcgtcggtga gccacgccgg ccaggcgcgc ggcaccgggt gggccagcgc catcgcccgg
     5341 gcgaaggcac gctgccgggc caccgccgaa cgcaccgcgg tccgggccct gttcgccggt
     5401 gcgggggcgg cggcaccgga gtcggcgagg accgaccagg ccgccagccg gccctcgtgc
     5461 gcggcgagtg ccgccccgcc cacgccgcac gtctcacccg cggtgtagag accggggacg
     5521 gtggtgcgct gccccgcgtc gacggcggcc accgcgttgc cgtccgccga ctcggtgagg
     5581 gcgcagccga gggggacgag caggtcgagc tggggggcga agccccagcc gacgcccacg
     5641 gcgtcgacct cgacccggcg ttccgtaccc gggcgcgggc tgccgttgga cgccagcgcg
     5701 gcgaggcgca ccgccgtcac acgtgcgccg ccctccgcgg cgacgatcgc cgtgcgcggg
     5761 cggaccggga cgcggtggcg ggcgagcgtg gcggcgtagg aggccccctc ggcccacttg
     5821 ccggggttgc gcagcagcgg gccggggtgg cgcagccagg cccgggggtg ggccgcctcg
     5881 cacaccgcga cgacctcgac gccgcgcgcg gcgagaccgg cggccaccgg cagcaggaac
     5941 ggcccggtgc cgcccagcgc cacgcggctc ccggccgcca cgccgccgcc cttgagcagc
     6001 gcctgcaggc cgcccgcggt caggactccg ggcaggtccc agcccgggaa gggcagttgc
     6061 cggtcgtagg cgccggtcgc cacgaggagc ctcggcgccc gcaggacggc cgcggtctcg
     6121 cgcggcgcgt cgcggcggtc gaccacgtgg accgcgaatc cgtcgccctc gcggacggcc
     6181 gaccacgcgt ggtgcgccag gcgcaggtcg agccggcccg ccgagcggtg tgccgccaac
     6241 gcccggcaca gggcacggta ccggcccagg ccgtggtgca ggtcgtcggt cggtacggcc
     6301 gctcgcgcgt gctccggcgg gtgccgccag tactggccgc cgagggccgt tccggagtcc
     6361 agcaggacga cgcgcagccc accggcgagc gccgtggccg cggcggtcat gcccgccggg
     6421 cccgctccca cgacgacgag gtccacgggc tcactcatcg tcgtcctccc gcgtcgtgac
     6481 ggtcatgccc ggccgggccg ggagcaggca ggcgcgctgc ccgcgggccc cgtcgaccgt
     6541 gacgaggcag tcgaagcaga cgccgatgcc gcagaagacg ccccgggggc gccgtccgac
     6601 gcgggtggtg cgccaggcga cccggcccgc ggcgacgagg gccgcggcga cggtctgtcc
     6661 ctcgacgaac ggcagggggt cgccgtcgac gacgatctcg ctcactgggg cgcacctccg
     6721 ggggtgagga agcggtcggg caggagaccg gtgtgcgcgg ccgggtcggc gccgcgccag
     6781 ggccggccga gcaggtgggc cgtcaccagg gcgccggtcc cgggggcgag gccgatgccc
     6841 gccccctcgt ggccgcaggc gtggacgacg cccggcacgc gcgggtcggg gccgatgacc
     6901 ggcaggtggt cgggacagta cggcctgaag ccccggtagg cgcgcatcag gtggacgccg
     6961 cgcaggaagg ggaagagccg gcacgcctgt gcggcgagcc gggcgaccac ggcggtgttc
     7021 aacgtcgtgt cgaagccgac gcgctcccgg ctggcgccga cgaggatcgt gccgccgcgc
     7081 gttccctcca cgacgcagga ggtctccagg ccggcgtcgg aggaggcgac gttggcgacg
     7141 tagtcggcgg agtagacctt gtgccggacc atcggcggca gcggttcggt caccaggacg
     7201 aagccgcggc gcggcaacac ctcgacgggc gcaccgagcc gccggccgac ctcgccgccc
     7261 caggtcccgg cggcgttgac gacggcgtcg gcgggcagga cctcaccggc ggccgtccgg
     7321 acgccggtga tccggccgcc ggggaccggg cctacgggga ccgggcctac gggggccggg
     7381 cctacggggg ccggaccgtg cgaggtcggg ccgtgcgggg tcggacccag cggggtgcgg
     7441 tcgccgtcgc gggtggtcag ggcgccgacg acctcccccg tgtggaagcg cgctccgcgc
     7501 cgtacggcgg cgcgcagcag ggcggccgcc gccagcaccg gctggacctg cgcgtcctgc
     7561 gggtagtgca cgccaccggg gatcccgggg gcgaggtggg gttcgaggtc ccgcacccgg
     7621 tcgaccggct cggcacggac accggccgcc tcctgccgcg cggcgaactc acgcagcgcg
     7681 gcgaggctct cgtcggtgcc cgcgacgacc aggccgccct tgggctccag ttcgagggtg
     7741 tcggctccga gttcgcggcc cgcctcgtcc cacagggtgc ggctcaaccg ggccagggcg
     7801 agttcgggac cgggttcctt gtcggagagg aggacgttgc cctctccccg gctcgtcgtc
     7861 ccggcgccca cgggtccgcg gtcgacgacc gtgacgtcca ggcccgccga ggcggcgtgg
     7921 aaggcgcagg cggctccgac gactcccgcc cccacgacga cgaccttgag ggtcaacggc
     7981 tcctccatcc atgcgtcaca ccgcgctgtg caatgtcaca tgccatgggt gaggctatcc
     8041 atccggtcgg ccgaagacaa cgcctccttc ggacccggac gtcagaggtc gacgcgggtg
     8101 cccagcccgg tcagggcgcc gaccgggtcc agatcggggg taccgcgcgg ccaccagtcc
     8161 tcgcggccgg gctccgactc gtacgcgtac cagagaccgg tccggccgag tctcagctgg
     8221 acatggcccc gggggtgggt gaggcggttg cgccaggggc ggaaggcggg gaggtcggcg
     8281 gcgaggagca gggggcgggc gcggtcgaaa cggccggccg gcgggtccca gggctcctcc
     8341 aggacgtcga ggccctccgg cccgccctgc cgccaggcgg cgacggcgcg cgccagctcc
     8401 gccgtgtcgc gtccggcggc cgaggcgagg gacgcgtaga gggcgcgggt gcccgcggtg
     8461 agcccggagc cggggcgcgc cgcggcgagg cggacggcgt cctgccacag cgtcagcccg
     8521 ccgaccgggt cgcgcccggt ggtgagcagg gcgtgggcgc gggcggcggc gtcggtggcg
     8581 agctggtcca gcgcgaagga gtcggggccg cccggcgcgg tcggacaggc cggtggctgt
     8641 tccgggtgcg ggggcgcggg caacggcgcg gggaggggcg gcaggacgcg ccgggccagg
     8701 acctccccgg cccgcacgcc gggcaggggg gccggtccgc cgtcctgggc ggtacgggcg
     8761 gcgcgggccg cgttgcgccg ggacagggcg tcgagcagct cgcgctcgcc ccggccgcgc
     8821 agcaggagca gtacgaaggg gtcggcgtcg agcagacgtg ccgtctggta gcagagggcg
     8881 gcggcgtgct tgcaggggtg gccggagtcg gggcaactgc actgcggggc gaggtcgccg
     8941 gggccgggga gcagcgggac gccgtggtcc gccaagtcgg ccagcgagtg cgggagttcc
     9001 ttgtcgagga gggcggcgat gtgtccgggc cgttcgacgg cggcgtccag gaagcggtcc
     9061 cagtcggcgt cgccgagcgt ccgcagccgc acctgcacgc ggtacggccg ggcgcggctg
     9121 ccctgcacgt aggcgaggac cagcccgggc gtcacggtga tggcgtccac gcgcccacgc
     9181 tcggcgtacc cccgcccgcg ctccagcctc gcggggtcca gggcgccctc ctccagggcg
     9241 gtcacccacg cgttccccca ccaggtgccc gcgaggccct cgtccgccac cgcacggggc
     9301 ggaagcgcgg gaaaggtccg ccgcagctcg ccgtcgcggt ccggggccgc catcgagcgg
     9361 ggggcgcggg gtcgtgcggg cggcggtgcg ggtggcggtg cggggtgggc ggcggcccgg
     9421 tcgcgcaccg ccgacgcctg ctccgggtgc ctccgccgtt ccggccgccc gggatcgggt
     9481 gccgggtctt cggagcgggg cgtgcgcccg gcgggaggag aggcggcggg tggggtgccg
     9541 gtcccggagt cggggcccac gccggacgac ggggccggtt ccggggacgc cgacgcccgt
     9601 actccacggg gagcccggcc ggcccgctcg tcggcgggca ggatctcccc gctctccccg
     9661 gtcttgccgg tctccccggc cgacgagatc tgcccggccg ggtccgcctc cgagggcagc
     9721 cggaaggcgc ccgcgacgaa gtcccgtacg gcacgtgcgc gggcgtcggc ggtgcccgcg
     9781 ccgcccgccc gccgccgacc gcctgccgga cggggcgccc gccgtgggtg cgcgacgccc
     9841 ttggccgacg cgtccttggc cgacgcgacc tcggacgccg cgtccgcctt cgccttccga
     9901 cgcgccgctc gcaacgcctc ccgcgccacg tccgccggac ggctgtcgcc cccttccacg
     9961 gcaccggagc gggacgcttc gtcgggggcg gtcggggcgg tcggggcggt cggggtggtc
    10021 gcgtcgtcgg accggtcccc ttcctctgcc cgccgggccg agtcccccgg gtccgccgaa
    10081 cccggcggac gtgccggact cgccggctcc gccccacctg ccggcccctc ccggtgtgac
    10141 ggtgcgtcac gggcgccggc ggggtcgtgc gcccccgcgt gccgtgtcct gcgcagggcg
    10201 gcccgggcgg tgtccccggg gcgtggcccg gagggctcgg cgggcctgcc gtccggggcg
    10261 cgctcgtggc tgtgcgccgg cgccgagggc gcggacggtg ccgcctccgc cggcgcctcc
    10321 gacgaggtcg ccgccggtgc cgccggcccg tgctccccgc ccggtccccc cgcgcgctca
    10381 cgtgcctccc gcagggcgcg gcgggcctcg tcggcgggcc gggggctcat gacggcctcc
    10441 gcagggagac gaggtcggac agttcacggg ccgtcagctc agtgagggcc gcctctccgg
    10501 agccgaggac cgcgtcggcc agggcccgct tggactggag catctcggcg atgcggtcct
    10561 cgaccgtgcc ctcggtgacg agccggtgga cctggaccgg ctgggtctgg ccgatgcggt
    10621 aggcgcggtc ggtggcctgc tcctccaccg ccgggttcca ccagcggtcg acgtgcacga
    10681 cgtgtcccgc gcgggtgagg ttcaggccgg tgcccgcggc cttcagggac aggacgagga
    10741 ccggggtcgc cccgctctgg aagcggtcca ccatccgctc gcgctccggg accggcgtac
    10801 cgccgtgcag caggtcgacc gggaccgcgc gggcggtgag gtgggcggtg atgaggcggg
    10861 ccatgccgac gtactgcgtg aagaccagca ccgagccgtc ctccgcgagc accgtgtcca
    10921 acagctcgtc cagcagggcg agtttgccgg agcgggcggt catccggtcg gccccgcccg
    10981 gcgggtgctc ctccttcagg aacagggcgg ggtggtcgca gatctgcttc agcgaggtca
    11041 gcagcttgag gacgagaccg cggcgcccca tgccctcggc ggcctcgatg gcgagcatcg
    11101 actcgcgcac caccgcctcg tacagcgcgg cctgttcgcg ggtgaggggg acggggtggt
    11161 ccgtctcggt cttcggcggc agctcgggga cgatgccggg gtcggacttc ttccggcgca
    11221 gcaggaaggg gcggatcagc cgggccaggc gctccacggc ctggtcgtcc tcgccgttct
    11281 ccaccgcgcg cgcgtgccgg gcgcggaagg acttcagggg gccgagcagc ccgggggtgg
    11341 tccagtcgag gagggcccac agctcggaga ggttgttctc cacgggtgtg ccggtcagcg
    11401 ccacccgggc cggggacggg atcgtgcgca gggccttcgc cgtcgccgag taggggttct
    11461 tcacgtgctg ggcctcgtcc gcgacgacca tactccagga ccggccggcc agggccgcgg
    11521 cggcggaccg catcgtgccg taggtggtga ggacgaagcc gccggtcagg tcgtcgaggg
    11581 tgcggtcggg gccgtggaag cgacggaccg ggacgccggg ggcgaaccgc tcgatctcgc
    11641 gctgccagtt gcccaggaga gaggccgggc agacgaccag cgtcggttcg gtgcgggccc
    11701 gcttcaggtg cagggcgatg acggtgacgg tcttgccgag gcccatgtcg tcggcgaggc
    11761 agccgccgag gccgagcgag gtcatgaggt cgagccaggc caggccgcgc agttggtagt
    11821 cgcgcagggt ggcgtgcagg ccgggcggcg cttcggcggg gcgcacaccc gcggtcagcc
    11881 ggtcgcgcag cgcggccagc gcgccgacgg gcaccacgtc gaccgtctcg ccgtcggcct
    11941 cggcactgcc ggtgagggcg acggcgaggg cgtcgaccgg gtcgagcaga ccgagatcgc
    12001 gcttgcgggc ccggcggacg agcgccgggt cgaccagcac ccaccggtcg cggagccgga
    12061 cgacggggcg gtgggcctcc gccagggtgt ccatctcggc ctcggtgagc gggtcgccgc
    12121 cgatcgcgag ctgccagcgg aactggagca ggtcctcgct ctcgaagaag cccgtgccgt
    12181 cggtcgccga acccggcgcg gtcctgacca ccgcggtcgc ggtgaggtcc tgggccaggt
    12241 cgcggggcca gtgcacggcg acgcccgcgg cggcgagccg gccggcggcc acgcccagca
    12301 ggtcggtgac ctcctcctcg gacaggccga gcacgtcggg cacgtcctgc tcggtgagcc
    12361 ggtccagcgg cggccagacc cgcgccgcgc gccgcaccgc gagggcggcg tccacgcgcg
    12421 cgcgggggcc gaaggaggcg tcggccgtgc cggaccagag gtccgcggcg tcggcgacga
    12481 gggtggggtc ggcgaggctg tgcacctgga cgaccgccgc gcccgcgttg cgcacgccgg
    12541 tgggtccgcc gccctccgtg cccgcggctc cgtccgcgtc ccggtcgaac aggtcgtacg
    12601 ccgacaggtc caggcggagc gagatccgca cgcccgcgtc catgcccgcg gccacctccg
    12661 cggcccagtc gtgggcgtcg ggcaggcgct gggccccgcg ggcggcgaac ggccgtccgc
    12721 aggcgtgcgg tgcggccgga gtgcggggca gggtgtcggc gaccgcgtcg aggaaggccc
    12781 ggaccagtgc ttccggctcc ggcagccgga tcggtcccgg cccgtcgagc gggacggcgt
    12841 ggccctcggg gggcagggcg gcggccacgg cccgcaggtg cgcgacgtcg tcggggccga
    12901 gcgggccggc ccgccaggcg tcgtgtccgg tcgcggtcag gccgggcagc agccggccgc
    12961 gcgcggtgag ccgcagggcg tgcagggc
//
