LOCUS       NZ_BBOK01000013        10399 bp    DNA     linear   CON 17-AUG-2024
DEFINITION  Streptomyces sp. NBRC 110030, whole genome shotgun sequence.
ACCESSION   NZ_BBOK01000013
VERSION     NZ_BBOK01000013.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. NBRC 110030
  ORGANISM  Streptomyces sp. NBRC 110030
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Katano-Makiyama,Y., Hosoyama,A., Hashimoto,M., Tsuchikane,K.,
            Uohara,A., Kitahashi,Y., Ohji,S., Ichikawa,N., Kimura,A.,
            Yamazoe,A., Komaki,H., Igarashi,Y. and Fujita,N.
  TITLE     Whole genome shotgun sequence of Streptomyces sp. NBRC 110030
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 378059)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 97x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417675.1-RS_2024_08_17
            Annotation Date                   :: 08/17/2024 02:44:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,152
            Genes (coding)                    :: 7,019
            CDSs (with protein)               :: 7,019
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 45 of 133
            Pseudo Genes (incomplete)         :: 107 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 23 of 133
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:33:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 299194
            Orig. end                         :: 309593
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBOK01000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(666..680)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     gene            1444..2052
                     /locus_tag="APK71_RS29495"
     CDS             1444..2052
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="APK71_RS29495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_031086688.1"
                     /transl_table=11
                     /translation="MDLDLPELPFSLRAYGPDGHWSHRDGVLTGRAGPRQDRFVPPTGE
                     SLESVSDAPRLLGSPEGDFQLVARVTVGFRGAFDAGVLYVHVGERAWAKLCLEYSPDVA
                     TVCTVVTRGHSDDCNSFTVEGNSVWFRVSRSGRAFAFHASPDGERWTFVRLFTLGDEEE
                     TGAALVGFLAQAPTGEGCTVTYDHLEFRPGWPADLRDGS"
     gene            2140..2853
                     /locus_tag="APK71_RS29500"
     CDS             2140..2853
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329309.1"
                     /locus_tag="APK71_RS29500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_053638634.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALASFPHRDAVEVVHRSFELDP
                     GRAKDDVQPVLTMLTAKYGMSEAQAQAGEDNLGAQAAAEGLDYRTRGRDHGSTFDMHRL
                     LHLARERGRHEALLDAFYRGNFADERSVFNDDERLEELAVGAGLDAGEVRAVLADPDAY
                     ADAVRADEREAAQLGATGVPFFVLDRAYGVSGAQPAEVFAQALTQAWGGRTPLKLIDDG
                     DAEACGPDGCAVPGR"
     gene            2925..4019
                     /locus_tag="APK71_RS29505"
     CDS             2925..4019
                     /NRPS_PKS="Domain: Aminotran_5 (29-358). E-value: 9.9e-24.
                     Score: 75.8. Matches aSDomain:
                     nrpspksdomains_APK71_RS29505_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448151.1"
                     /locus_tag="APK71_RS29505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_107094393.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.3e-18, bitscore:
                     61.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGEIRGPHSGAMESFENLVRAEFAPKTTYLNTASTGLLPARSLV
                     AMRDAAAAAAAGQPLDIFADVEASRAAYARLVGVPATRVATGASVATFSGLVAASLPPG
                     AEVLTAEADFSSLVNPLHARGDLKVRSVPLERLAESVGPATALVAVSSVQSADGRIADL
                     AALRDAARAHGARTLIDVSQANGWLPTAADDFDFVTAVGYKWVLCPRGVAFLVVPEDLG
                     AAAGLTPRFAGWATGERPWESCYGPVEEYAHSARRFDESPALFSYTGARHSLALIEELG
                     VDAVHAHDTALADRFRAGVRALGHEPVPAPGSAVVSVPGLGHRQGGLGEAGVQVSDRAG
                     NLRAAFHVYNTESDVDRLLDVLAG"
     aSDomain        3012..3998
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_APK71_RS29505_Aminotran_5.1"
                     /evalue="9.90E-24"
                     /label="APK71_RS29505_Aminotran_5.1"
                     /locus_tag="APK71_RS29505"
                     /protein_end="358"
                     /protein_start="29"
                     /score="75.8"
                     /tool="antismash"
                     /translation="YLNTASTGLLPARSLVAMRDAAAAAAAGQPLDIFADVEASRAAYA
                     RLVGVPATRVATGASVATFSGLVAASLPPGAEVLTAEADFSSLVNPLHARGDLKVRSVP
                     LERLAESVGPATALVAVSSVQSADGRIADLAALRDAARAHGARTLIDVSQANGWLPTAA
                     DDFDFVTAVGYKWVLCPRGVAFLVVPEDLGAAAGLTPRFAGWATGERPWESCYGPVEEY
                     AHSARRFDESPALFSYTGARHSLALIEELGVDAVHAHDTALADRFRAGVRALGHEPVPA
                     PGSAVVSVPGLGHRQGGLGEAGVQVSDRAGNLRAAFHVYNTESDVDRL"
     gene            complement(4098..4994)
                     /gene="thpD"
                     /locus_tag="APK71_RS29510"
     CDS             complement(4098..4994)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="APK71_RS29510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_030403832.1"
                     /sec_met_domain="PhyH (E-value: 6e-43, bitscore: 142.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTNVTDLYPTRGATEVATPRQDPVVWGSPDTPGPVSASDLQS
                     LDRDGFLAIDQLIAPEEVAEYQRELERLTTDPAIRADERSIVEPQSKEIRSVFEVHKIS
                     EVFAKLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPH
                     MRTISVSIALTENFDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEAL
                     TKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFAA
                     PIRRPEFIGARDFTPVK"
     misc_feature    complement(4985..5005)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.15"
                     /tool="antismash"
     gene            complement(5001..5399)
                     /locus_tag="APK71_RS29515"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="APK71_RS29515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_030184764.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.8e-55, bitscore:
                     180.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEFEGTDRHVKSASGTWESTRIVLAKEKVGFSLHETILY
                     AGTETSMWYANHIEAVVCTKGDAELTDRETGKTYHITPGTMYLLNGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            complement(5514..6785)
                     /gene="ectB"
                     /locus_tag="APK71_RS29520"
     CDS             complement(5514..6785)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 4.9e-72.
                     Score: 234.7. Matches aSDomain:
                     nrpspksdomains_APK71_RS29520_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="APK71_RS29520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_030403833.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.1e-77, bitscore:
                     253.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAVGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNAVLKRALIDYLERDGVTHGLDMSTTAKRRFLETFQNTILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFIWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALADLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATATLETYWTDGSAMEKQTRTRGEQVEQHM
                     IAITEENLADVKEYRGRGLVWGLEFHDKGRAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGMKTLARAVRETV"
     aSDomain        complement(5688..6698)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_APK71_RS29520_Aminotran_3.1"
                     /evalue="4.90E-72"
                     /label="APK71_RS29520_Aminotran_3.1"
                     /locus_tag="APK71_RS29520"
                     /protein_end="366"
                     /protein_start="29"
                     /score="234.7"
                     /tool="antismash"
                     /translation="DRAVGSRMYDEDGHEYLDFFAGAGSLNYGHNNAVLKRALIDYLER
                     DGVTHGLDMSTTAKRRFLETFQNTILRPRDLPYKVMFPGPTGTNAVESALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFIWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALADLCERQDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATATLETYWTDGSAMEKQTRTRGEQVEQHMIAITEENLADVKEYRGRGLVWGLEFH"
     gene            complement(6913..7425)
                     /gene="ectA"
                     /locus_tag="APK71_RS29525"
     CDS             complement(6913..7425)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448158.1"
                     /locus_tag="APK71_RS29525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_030403834.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPRVADGAALWRIARDSKVLDLNSSYSYLLWCRD
                     FAATSAVARDENGEPVGFVTGYVRPDRPDTLLVWQVAVDEAYRGRRLAAALLDGLAERT
                     VAERGLTTLETTISPDNTASQRLFTSFAERRGARVEREVLFDAGVFPDGPHEPEVLYRI
                     GPLSATG"
     misc_feature    complement(7424..7436)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7581..7596)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(7591..7608)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.57"
                     /tool="antismash"
     gene            complement(7755..8882)
                     /locus_tag="APK71_RS29530"
     CDS             complement(7755..8882)
                     /NRPS_PKS="Domain: Aminotran_5 (20-291). E-value: 5.2e-29.
                     Score: 93.2. Matches aSDomain:
                     nrpspksdomains_APK71_RS29530_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976957.1"
                     /locus_tag="APK71_RS29530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_031186144.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1e-22, bitscore:
                     75.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAGRFAAIEDRIARLLGTAQDVVVMQGEALLPLE
                     GAIRAAAGPGTVALNVVTGPYGQTFGDWLRDCGATVHDLAVPFHTAVSAERIGQALAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPHAPRRSYLSLLDWKERWIDGGRKALLHAP
                     AQLEMLALEACVERIETEGAGAVMARHASAAAATRAGALALGGLEPFVHEAKDAAPVAT
                     TLRTPSDVDASALVARALESDPALPLAAGGGALAGEMIRVNHYGPHATPGAVQGCLAAL
                     GAALAERGGKADPEAARRAAEEAWRRPAGTSPSEG"
     aSDomain        complement(8010..8822)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_APK71_RS29530_Aminotran_5.1"
                     /evalue="5.20E-29"
                     /label="APK71_RS29530_Aminotran_5.1"
                     /locus_tag="APK71_RS29530"
                     /protein_end="291"
                     /protein_start="20"
                     /score="93.2"
                     /tool="antismash"
                     /translation="DRIARLLGTAQDVVVMQGEALLPLEGAIRAAAGPGTVALNVVTGP
                     YGQTFGDWLRDCGATVHDLAVPFHTAVSAERIGQALAEHPEIDFVSLVHAEAATGNTNP
                     VAEIGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSAVSVSE
                     RAWARMAANPHAPRRSYLSLLDWKERWIDGGRKALLHAPAQLEMLALEACVERIETEGA
                     GAVMARHASAAAATRAGALALGGLEPFVHEAKDAAPVATTLRTPSDVDA"
     gene            9080..9616
                     /locus_tag="APK71_RS29535"
     CDS             9080..9616
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="APK71_RS29535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_051783643.1"
                     /transl_table=11
                     /translation="MLALLLLVLVAVVTAVVLRRGAGAYPRHLAADAVAAAPVPRRPGA
                     PFRAVAGAAGGAAVLLYLWGVLCVALAVMSAEDGGADSSPPRPCRTGVSPQLSGTVVGH
                     SASYLPLGLRCETADGGGYDSADVPGYLNPAVAALALTAVGSGVAAGCATERRVRAAAR
                     GGGPSPASDRPTGSP"
ORIGIN
        1 ccccgccggt cgtcccccgg aagtcgtcgt ccgcgacgac cgaagtcgca ccgttcgcgg
       61 gggagcgctg cccgtcgtcg ccgtacggca gcacaccggc gagccggaag cccccgtcgg
      121 gggtggggcc gctgtgcacc atgccgccga ccagccgggc ccgctcccgc agacccgtca
      181 gcccctggcc gccgctgatc gcgggcggcc cggccggtgc cccggccggc accggcccgt
      241 tggcgacctc caccacgagg ctgtccggtt cgtagcgcag cgccacggtg atcggggcgc
      301 ccggcgcgtg cttgtgcgcg ttggtcaggc cctcctgggc tatgcggtac gccgcgcggt
      361 cggcggcggg agcgaggggc cgttcggtgc ccgaccggcg cagttccacc gccgtccccg
      421 cggcccgcga cgacgcgacg aggccgtcga cggaggccgc cgcgcggtcc gacgacgcct
      481 ccgtcccctc ccggccgggc acggacacct ccgcgtcgtc ccgcaggatg cccaccgcct
      541 cgcgcagctc acacatcgcc gacctggcgg cctcccgcag gatgcccacc ccttcgcgcc
      601 ggcggccgtc gaggtccggg tccacctcca gcgcgccggt gtgcacggcg atcagggcga
      661 gctggtggcc gaggctgtcg tgcatgtcct gggcgatgcg ctgccgttcc agcagccggg
      721 cctgccgcgc caccatggcc tgctcgcgca ccagttgggc gttgtgccgg cgcagggcgt
      781 cgagcagggc acggcgctgg gcgcggtacc gggcggcgaa ccccggcacg accgccagca
      841 ccacgaacgg gctcgcggcc agcgcggccg tcatcggcag cgacgtgccg agcccgtcgt
      901 cgcccgcggt ggcggcctcg gcgacgaggt ggaggacgaa cccggccgcg aacgcggcca
      961 gggcgcgcgc cgggcgctcg atgcggcccc ccgccgacca ggcggcgcag acgagcagcg
     1021 cgaacgtacc gaggaccgcc ccggccagtg ccgacgagac caccagcacg gtcgccggca
     1081 gggcacggcg ggccagggac aggacagcga cggccagggc cagggcgccg cccagcaccc
     1141 cggacccggt gctcgtcgtg ccgagcagac cggcgaccgc ggagagcagc gaggcgagca
     1201 cggcctcccc ggcgatccgc ccgcggggcc aggaggaggc ggtgaccggc cgggtgagga
     1261 accgcaggcc ggtgcggggt gtctctgcta cgtccacgcg cagacgatag agggccgtcc
     1321 ctgggccccc gcgtcctccc ttcggcgcgg tcccggacga cgaaagtgga ctcccggcgg
     1381 agccgtaccg gccgcaccgc ccccgaagct ccggcgcccg gccccgcctg gaagaatccc
     1441 gccatggacc tggacctccc cgaactgccc ttctccttgc gcgcctacgg ccccgacggc
     1501 cactggtccc accgggacgg cgtcctcacc ggacgggccg gcccccggca ggaccgcttc
     1561 gtgccgccca ccggggagtc gctggagtcc gtctccgacg cgccgcggct gctgggctcg
     1621 cccgaggggg acttccagct cgtcgcccgc gtcaccgtcg ggttccgcgg tgcgttcgac
     1681 gccggcgtgc tctacgtcca cgtgggggag cgggcctggg ccaagctgtg cctggagtac
     1741 tccccggacg tggccacggt ctgcacggtg gtcacccgcg gccactccga cgactgcaac
     1801 tccttcaccg tcgaggggaa ctccgtctgg ttccgggtca gccggagcgg ccgggccttc
     1861 gccttccacg cctccccgga cggcgagcgc tggaccttcg tccgtctctt cacgctcggc
     1921 gacgaggagg agaccggcgc ggccctcgtc ggattcctgg cccaggcgcc gacgggcgag
     1981 ggctgcacgg tgacctacga ccacctggag ttccggccgg ggtggcccgc ggacctgcgg
     2041 gacggaagct gagggcggga atgcgaccgg ctggttgaag gttcacgcaa ggggcggaga
     2101 aggactccgc cgccgtacga gccggagaag agccgagaca tgcgcgtcga aatctggagc
     2161 gacatcgcct gcccctggtg ctacgtgggc aaggcccgct tcgagaaggc gctcgcgtcc
     2221 ttcccgcacc gggacgcggt cgaggtggtc caccgttcgt tcgagctcga tcccgggcgg
     2281 gccaaggacg acgtgcagcc ggtgctgacc atgctcaccg ccaagtacgg catgagcgag
     2341 gcccaggcgc aggccggtga ggacaacctc ggtgcgcagg ccgccgccga gggcctggac
     2401 taccgcaccc ggggccgcga ccacggcagc accttcgaca tgcaccgcct gctgcacctc
     2461 gcccgggaac ggggacggca cgaggccctc ctcgacgcct tctaccgggg caacttcgcc
     2521 gacgagcgct ccgtgttcaa cgacgacgag cggctcgagg agctcgccgt cggcgccggt
     2581 ctggacgccg gtgaggtccg tgcggtgctc gccgaccccg acgcctacgc cgacgccgtg
     2641 cgcgccgacg agcgggaggc cgcgcagctc ggggccaccg gggtgccgtt cttcgtgctc
     2701 gaccgggcct acggcgtctc gggagcccag cccgccgagg tcttcgccca ggcgctgacc
     2761 caggcgtggg gcgggcgcac gccgctgaag ctgatcgacg acggggacgc ggaggcctgc
     2821 ggcccggacg gatgcgcggt gcccgggagg tgacgggtgc gccgtcgtcg caggtgggcg
     2881 gcggtacata agcgttgctt ggagacaccg cgtagaaaga ggcgatggac ggggagatcc
     2941 gggggccgca cagtggtgcc atggagagct tcgagaacct cgtccgtgcc gagttcgcgc
     3001 cgaagaccac gtacctgaac accgccagta ccggcctgct ccccgcccgg tccctcgtcg
     3061 cgatgcgcga cgcggcggcc gccgcggcgg cggggcagcc gctcgacatc ttcgccgacg
     3121 tcgaggcgtc ccgggccgcg tacgcccgcc tcgtgggggt gcccgccacc cgggtcgcga
     3181 ccggcgcgtc cgtcgccacg ttcagcgggc tcgtcgccgc gtcgctgccg ccgggggccg
     3241 aagtcctgac cgcggaggcc gacttcagct ccctggtgaa cccgctgcac gcccggggcg
     3301 acctcaaggt gcgcagcgtc ccgctggagc ggctcgccga gtcggtgggc cccgccaccg
     3361 cgctcgtcgc ggtcagttcc gtgcagtccg ccgacgggcg gatcgccgac ctcgccgcgc
     3421 tgcgggacgc cgcccgggcg cacggggcac ggacgctgat cgacgtctcc caggcgaacg
     3481 gctggctgcc gacggccgcg gacgacttcg acttcgtgac cgccgtcggc tacaagtggg
     3541 tgctctgccc gcgcggcgtc gccttcctcg tcgtaccgga ggacctgggc gcggcggccg
     3601 ggctgacacc gcggttcgcc gggtgggcga ccggcgaacg cccctgggag agctgctacg
     3661 gcccggtcga ggagtacgcg cactcggccc ggcggttcga cgagtccccc gccctgttct
     3721 cgtacaccgg cgcccggcac tccctggcac tgatcgagga actgggcgtc gacgccgtgc
     3781 acgcccacga caccgccctc gccgaccggt tccgcgcggg ggtgcgcgcg ctgggccacg
     3841 agccggtgcc cgcgccgggc tccgccgtcg tgtcggtgcc ggggctcggc caccgccagg
     3901 gcgggctggg cgaggccggc gtccaggtct ccgaccgggc gggcaacctg cgggccgcct
     3961 tccacgtgta caacaccgag tccgacgtgg accggctact cgacgtgctg gcgggctgag
     4021 cacagcggct gcggacagca tcgggcaggg gcctcccgtc ccacacgaaa aggcccctgc
     4081 ccgagatcag acgcgggtca cttcaccggc gtgaagtccc gcgcgccgat gaactccggc
     4141 cgccggatcg gggccgcgaa cggctccacg gccgtgttct ccacgctgtt gaacacgatg
     4201 aacacattgc tgcgcgggaa cggcgtgatg ttgtcgcccg acccgtgcat gcagttgcag
     4261 tcgaaccacg tcgccgagcc ggccttgccg gtgaacagct tgatgccgta ctcggaggcc
     4321 atcttggtca gcgcctcgtc ggacggcgtc cccgcgtcct gcatctgcag ggacttcttg
     4381 tagttgtcct tcggcgtggc ccccgcgcac ccgaggaacg tcttgtgcga ccccggcatg
     4441 atcatcaggc cgccgttggt gtcgaagttc tccgtgagcg cgatcgagac cgagatcgtg
     4501 cgcatgtggg gcaggccgtc ctcggcgtgc caggtctcga agtccgagtg ccagtagaaa
     4561 ccgctggccc cgaagcccgg cttgacgttg atccgcgact ggtggacgta gacgtccgag
     4621 ccgaggatct gccgggcccg cccgaccacg cgctcgtcgc gcaccagctt ggcgaagacc
     4681 tcgctgatct tgtgcacttc gaagaccgac cggatctcct tggactgcgg ctccacgatc
     4741 gagcgctcgt ccgcgcggat ggccgggtcc gtggtgagcc gctccagctc gcgctggtac
     4801 tcggccacct cctccggcgc gatgagctgg tcgatggcga ggaagccgtc gcggtccagg
     4861 gactgcaggt cgctcgccga gaccggaccg ggcgtgtccg gggagcccca gacgaccggg
     4921 tcctgccggg gggttgccac ctcggtggcg ccgcgggtgg gatagagatc agtgacgttc
     4981 gtggtcgtgg tcacggtgac tcacacctcc tcggtgagca gcgggtagac gccgttctcg
     5041 tcgtgatcct cccgtccggt gacgggcggg ttgaagacac agatgcagtg gaagtcctcc
     5101 ttgaccttga gcgtgtgccg ctcatggccg ttgaggaggt acatcgtgcc cggcgtgatg
     5161 tggtacgtct tccccgtctc gcggtcggtc aactcggcgt cgcccttggt gcagacgacg
     5221 gcctcgatgt ggttcgcgta ccacatcgac gtctccgtac ccgcgtacag gatcgtctcg
     5281 tgcagggaga agccgacctt ctccttggcg aggacgatcc gggtgctttc ccaggtgccg
     5341 gacgcgctct tcacgtgccg gtcggtgcct tcgaactcct tgaacgaacg gacgatcacg
     5401 gtgttgcgat gcctcctagg tgaaggatgt gcaagggtct ccggcgcctt cggccgcgcg
     5461 ggcggaaggc gccggaagaa tgggtggtgc cggacggccg ggccgtcgga cggtcagacc
     5521 gtctcgcgca cggcgcgggc gagggtcttc atgccctcgt ccagctcgtc gggcgtgatg
     5581 gtgagcgccg gcagcagctt gacgacctcg ctctcggggc cggaggtctc gatgagcagc
     5641 cccagctcga aggcgcgctt ggcgacccgg cccgcgcggc ccttgtcgtg gaactccagg
     5701 ccccagacca gaccgcggcc gcggtactcc ttgacgtcgg cgaggttctc ctcggtgatg
     5761 gcgatcatgt gctgctcgac ctgctcaccg cgggtgcggg tctgcttctc catggccgag
     5821 ccgtcggtcc agtacgtctc cagcgtggcg gtcgccgtga cgaaggccgg gttgttgccg
     5881 cggaaggtgc cgttgtgctc gcccggctcc cagacgtcca gctcgggctt gaacaggcac
     5941 agcgacatgg gcaggccgta gccgctgatc gacttggaga cggtgacgat gtccggcgtg
     6001 atgcccgcct cctcgaagga gaagaaggcg ccggtacggc cgcagcccat ctggatgtcg
     6061 tcgacgatga gcagcatgtc ctggcgctcg cacaggtcgg ccagggcccg cagccactcg
     6121 gcgcgggcca cgttgatgcc gccctcgccc tgcaccgtct cgacgatcac ggcggccggc
     6181 ttgttcaggc cggagccctg gtcctcgagg agccgctcga accagatgaa gtcctcgacg
     6241 gtgccgtcga agtagttgtc gaacggcatg ggcgtgccgt gcaccagcgg gatgccggcc
     6301 ccggcccgct tgaacgcgtt gccggtcacg gccagcgacc ccagcgacat gccgtggaag
     6361 gcgttggtga aggacacgat cgactcgcgc cccttgacct tgcgcgccag cttcagcgcg
     6421 gactcgacgg cgttggtgcc cgtcgggccc gggaacatga ccttgtacgg caggtcgcgc
     6481 gggcgcagga tcgtgttctg gaacgtctcc aggaagcggc gcttggcggt cgtcgacatg
     6541 tcgagcccgt gcgtgacgcc gtcccgctcc aggtagtcga tcagcgcgcg tttcaggacg
     6601 gcgttgttgt gcccgtagtt gagcgagccc gctccggcga agaagtcgag gtactcgtgg
     6661 ccgtcctcgt cgtacatgcg gctgccgacc gcacggtcga agacggtggg ccagccgcgg
     6721 cagtagctgc gcacctcgga ctcgacggtc tcgaagacgc tgaggtcggg ctgggtgatg
     6781 gtcacgacga atcgctcctc gtggggtgag ggtgggagag gaaggtcgta cggatccggg
     6841 gaccggtgcg gtccgccggg aagtctgcgg ggccggactg gcacggggtg ccgggccgtc
     6901 cggagggccc ggtcagccgg tggcggacag ggggcctatg cggtacagga cctcgggctc
     6961 gtgcggcccg tcggggaaca cgccggcgtc gaacagcacc tcgcgctcca cccgggcgcc
     7021 gcgacgctcg gcgaacgagg tgaacagccg ctgggaggcg gtgttgtcgg gggagatggt
     7081 ggtctccagg gtggtcagcc cacgttccgc gacggtccgc tcggccagcc cgtcgagcag
     7141 ggcggcggcg agccggcgcc cgcggtacgc ctcgtccacc gcgacctgcc acacgagcag
     7201 ggtgtccggg cggtccggac gcacgtatcc ggtgacgaag ccgaccggct caccgttctc
     7261 gtcgcgggcg acggccgacg tggcggcgaa gtcccggcac cagagcagat agctgtagga
     7321 ggagttcagg tcgaggacct tggaatccct cgctatccgc cacagggcgg cgccgtcggc
     7381 cactctcgga cgatcgattt gcaggtctgc ttgcgcggca gtcatgcaaa ttgaatttac
     7441 ccagcgaaaa tcgaaattgc atcgccgcat ggggttacga atgcatgcct cgtgtgttat
     7501 cacgcgggcg cgtgcttccg cgcgaggggg cggcgttttg ggatgatttg tggaggacga
     7561 aagccgcaaa gcgggcagcg tgtggatcgg gtcacaatcc cataaccggc tcgagacgtg
     7621 tccgaattgt gaagatcggc gttcgtgaaa gtgccgtgtt taggtcccgg gaaagcgggc
     7681 agaagaatac gggaagctgc gctgagtgga attccttaat tctacttgtg tgagaatcag
     7741 gaattccctc gaattcatcc ttccgaagga ctcgttccgg ccggccgccg ccacgcctcc
     7801 tcggcggccc gccgggccgc ctccggatcg gccttgccgc cccgctcggc cagcgcggca
     7861 cccagtgccg ccaggcagcc ctgtacggcc ccgggcgtgg cgtggggacc gtagtggttg
     7921 acccggatca tctcgcccgc cagcgcgccc ccgcccgccg ccagcggcag cgccgggtcg
     7981 gactccaggg cgcgggccac cagcgcggag gcgtcgacgt ccgagggcgt ccgcagcgtc
     8041 gtcgcgaccg gcgccgcgtc cttcgcctcg tgcacgaacg gctccaggcc gcccagcgcg
     8101 agcgcgcccg cccgggtcgc cgccgcggcg gacgcgtgcc gcgccatcac cgccccggcg
     8161 ccctccgtct cgatgcgctc gacgcacgcc tccagcgcga gcatctccaa ctgcgccggc
     8221 gcgtgcagca gcgccttgcg cccgccgtcg atccagcgct ccttccagtc caggagggac
     8281 aggtacgagc gccgcggcgc gtgcgggttc gccgccatcc gcgcccaggc ccgctcgctc
     8341 accgacaccg ccgacacccc ggccgggccg cccatcgcct tctgcgcgcc gatcacgcac
     8401 aggtccacgc cccacgcgtc cggcagcacc ggctcggcgc ccacggaggc caccgcgtcc
     8461 aggtagaaca gcgcgccgtg cgcccgcacc acctcgccga tctccgcgac cgggttggtg
     8521 ttgccggtcg ccgcctcggc gtgcaccagg gacacgaagt cgatctccgg gtgctcggcg
     8581 agggcctggc cgatccgctc ggcggagacc gccgtgtgga acggcacggc gaggtcgtgc
     8641 acggtcgcgc cgcagtcccg cagccagtcg ccgaaggtct gcccgtacgg ccccgtgacg
     8701 acgttcagcg cgacggtgcc gggacccgcg gcggcgcgga tcgcgccctc cagcggcagc
     8761 agcgcctcgc cctgcatcac caccacgtcc tgcgcggtgc ccagcaggcg cgcgatccgg
     8821 tcctcgatcg cggcgaaacg gccggcgctc aacggggcga gatccaggaa gggatgggtc
     8881 acggcggtgc tctcttcggt aacggcaggt gtacggcgcg ggtgcgcacg ggtacgacga
     8941 ggtacgtcgc gcacggagtc gagagtaacc gcggcacccg ccggcactcg cggcggaggg
     9001 cttccgttgc gacaccccca cgcccggaac catggcttcc gtcactgcgg agtgatcgtc
     9061 gggctgatgg actggccccg tgctcgctct tctgctgctg gtcctcgtcg cggtggtgac
     9121 ggccgtcgtc ctgcgcaggg gcgccggcgc ctacccccgc cacctcgccg cggacgcggt
     9181 ggccgcggct cccgtgccgc gccggccggg cgcgcccttc agggccgtcg ccggtgccgc
     9241 cgggggcgcc gccgtactgc tgtacctttg gggtgtgctc tgcgtcgccc tcgcggtcat
     9301 gtcggccgag gacggcggcg cggactcctc gcccccgcgc ccctgccgca ccggcgtgtc
     9361 tccgcagctg tccgggacgg tcgtcggtca ctccgcctcc tacctgcccc tcggcctgcg
     9421 ctgcgagacc gccgacggcg gcggctacga ctccgccgac gtccccggct acctcaaccc
     9481 cgccgtcgcc gccctggccc tgaccgccgt cgggagcggc gtggcggccg ggtgcgcgac
     9541 ggagcgacgg gtccgcgcgg cagcgcgggg cggcggcccg tccccggcct cggaccggcc
     9601 gacgggcagc ccttagggtg cggggcatga gcgatcgcgc ggtgctgcac gtgaagggtc
     9661 gggtgctcgt cggaccggag gaggtccggg acgaggtgtg ggtgatcgac ggccggatct
     9721 cctacgaccg gcccgccggc gcggccgacg tccgcaccgt cgaggggtgg gcgctgcccg
     9781 gcctggtcga cgcgcactgc cacgtcggcc tcgaccagca cggggccgtc cccgcggacg
     9841 tcgccgagaa gcaggcgctg accgaccgcg aggcgggcac gctgctcgtc cgggacgcgg
     9901 gctcgccctc cgacacccgc tggatcgacg accgcgagga cctgccgaag atcatccggg
     9961 ccggccggca catcgcccgc acccgccgct acatccgcaa ctacgcctgg gaggtcgagc
    10021 ccgaggacct cgtcgcgtac gtcgcccagg aggcccggcg cggcgacggc tgggtgaagc
    10081 tggtcggcga ctggatcgac cgcgacctcg gcgacctgtc ggcctgctgg ccgcgcggcg
    10141 ccgtcgaggc ggccatcgcg gaggcccacc gcctgggcgc ccgcgtcacc gcgcactgct
    10201 tcgccgagga ctccctgcgc gacctggtcg aggccggcat cgactgcgtc gaacacgcca
    10261 cggggctgac cgacgacacg atccccctgt tcgccgagcg cggggtcgcc atcgtgccca
    10321 ccctggtcaa catcgccacc ttccccgcgc tggccgacgg cggcgaggcc cgttatccgc
    10381 gctggtcggc ccacatgcg
//
