LOCUS       NZ_BBOK01000009        11317 bp    DNA     linear   CON 17-AUG-2024
DEFINITION  Streptomyces sp. NBRC 110030, whole genome shotgun sequence.
ACCESSION   NZ_BBOK01000009
VERSION     NZ_BBOK01000009.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. NBRC 110030
  ORGANISM  Streptomyces sp. NBRC 110030
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Katano-Makiyama,Y., Hosoyama,A., Hashimoto,M., Tsuchikane,K.,
            Uohara,A., Kitahashi,Y., Ohji,S., Ichikawa,N., Kimura,A.,
            Yamazoe,A., Komaki,H., Igarashi,Y. and Fujita,N.
  TITLE     Whole genome shotgun sequence of Streptomyces sp. NBRC 110030
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 320172)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 97x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417675.1-RS_2024_08_17
            Annotation Date                   :: 08/17/2024 02:44:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,152
            Genes (coding)                    :: 7,019
            CDSs (with protein)               :: 7,019
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 45 of 133
            Pseudo Genes (incomplete)         :: 107 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 23 of 133
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:33:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 193911
            Orig. end                         :: 205228
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBOK01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11317
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11317
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11317
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6317](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6317
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    574..589
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.67"
                     /tool="antismash"
     misc_feature    575..588
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.3"
                     /tool="antismash"
     gene            1326..2846
                     /locus_tag="APK71_RS22425"
     CDS             1326..2846
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133759.1"
                     /locus_tag="APK71_RS22425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_031085454.1"
                     /sec_met_domain="DAO (E-value: 2e-47, bitscore: 156.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQNVQVNGSYWLQTAPGGAPRPALGEDLDVDVAVIGAGVAGLSA
                     AWELVRAGRSVAVLEAGRVAAGVTGYTSAKLTVQHTLVYDRLRRTRGPEGARLYARSQS
                     EAIERAAGIVAELGVDCEWETRDAYTYVRDPGRVDEVRAEAAAAKEAGLPASFVTETGL
                     PFPVAGAVRVTGQAQFHPLKYLRALAADLEAHGGRIFEDTTVQGLEEGEPCRVRTEAGA
                     TVTARDVVVATHYPVFDRALLFARLSPRRELVVAGSIGADRDVDGMYITPEENTRSVRT
                     APLDDGRRLLVVTGEHFTPGTGDTRERFGTLAAWADEHFPGVERTHAWATQDIDPTDTV
                     PMVGPMHPGAHHTYVATGFGGWGLSGGMMAGRLLTAQITGEECAWSGLYDPRRLRTAVR
                     EAPALLKTQAEVARHFVGDRLRPVPPVDALPPGEGAVVRAGGDRLAVYRDEAGTLHAVS
                     ARCTHLGCLVGFNAAERAWECPCHGSRFDTDGKVIEGPATKPLEQRDI"
     misc_feature    complement(2896..2909)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.25"
                     /tool="antismash"
     gene            2940..3827
                     /locus_tag="APK71_RS22430"
     CDS             2940..3827
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445327.1"
                     /locus_tag="APK71_RS22430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_055468894.1"
                     /transl_table=11
                     /translation="MITLVRRATALCVLGAALTGCGTAETPQAPRPAPSAPSAPSSPSA
                     TASKPAPSGPPTLAPGPAGLTPVFEHGPRTDAAKDGKLVALTFDADMTADQGPRAAAGE
                     RFDNPALIATLRKLKVPATVFMTGRWAEEYPDEARSIGRDPQFEIANHSYSHYAYTEDC
                     YGLPTVSKDDMRADLERAYTAFEKAGVADPMPYFRFPGGCYDDAVRKELSATGVTAVQW
                     DVVSGDAFATDADAVTRQVLDGVRPGSVVVMHCTRSAAPVTEQVVRAVVPELRRQGYRF
                     AKVSELIGAADGHG"
     gene            3845..4072
                     /locus_tag="APK71_RS22435"
     CDS             3845..4072
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972872.1"
                     /locus_tag="APK71_RS22435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030404102.1"
                     /transl_table=11
                     /translation="MSSDHDPAPDGSGSGCDPGGYSLIGTAPHPPVADGPPYAECVLCR
                     EPTEYPESYKGITLCPVCEWQEAQRTACTG"
     gene            4139..4897
                     /locus_tag="APK71_RS22440"
     CDS             4139..4897
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030616.1"
                     /locus_tag="APK71_RS22440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_055468895.1"
                     /transl_table=11
                     /translation="MSNDPTAPAPVPVPDGPFRPEPGDRDLAPQFVLPLVVRIERAAPP
                     ARTDALETAARAVLVMLADARSTGDGAWARAMRDWQDARIRKVVRRARGAEWRRAGALP
                     GVTVTGKSAEVRVFPPVPLDGWPKDLARLQVSGTDLDDPEPPAGADPCAPVLWLNPGLD
                     MSAGKAMAQAGHGAQLAWWELSDGERAAWRDAGFPLSVRTADPARWPDLTAGGLPVVRD
                     AGFTEIAPGSCTVVADHPALRTPPGGAPGA"
     gene            5001..6317
                     /locus_tag="APK71_RS22445"
     CDS             5001..6317
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327585.1"
                     /locus_tag="APK71_RS22445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_031085461.1"
                     /sec_met_domain="DUF692 (E-value: 3.4e-100, bitscore:
                     328.6, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRLGTGIGWRPEIADVVERMPGIDWVEAVSENLCPGHLPDSLLR
                     LRERGVTVVPHGVSLGLGGAGRPDPGRLAALAERAEVLASPLVTEHIAFVRAGGAPTAS
                     PVLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALVAWPGEELTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNLGADPAEALAELPLEALAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTDLAARVAPPGVLLERDENFPEGDELRAEVEAIRDAVERGRTLRGPA
                     SGGGAAPRTPGPGSVPAAGDAVRQRLGLAQAALLSALVAGAPVPEGFDPVRLGVQARAL
                     AGKRADVVGKVAPELPVILGPRYRPAFLAYARGRPMTGGYRRDALDFVAHLLRTAPGDD
                     PGVRRALRAWWLERSGPVPRSSRPGVRLARAARRVLLRR"
     misc_feature    complement(6353..6370)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.84"
                     /tool="antismash"
     misc_feature    complement(6412..6425)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.47"
                     /tool="antismash"
     gene            6432..7229
                     /locus_tag="APK71_RS22450"
     CDS             6432..7229
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972877.1"
                     /locus_tag="APK71_RS22450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_055468896.1"
                     /transl_table=11
                     /translation="MFWVLFLLAAWAVAGVACLRLCLAAVRAAAVDPHAVVREHTLTLY
                     EAAFLSGGPRRVADLTLVSMARQRRLLLAHTGWATVVDPCGRDDMERSVIGAIGPQGQS
                     RIAPVRTAAATADAVRGLADRLVGAGLALPDGGTGGVAAGVRRVRAAAAAVCALGLVSL
                     ALPLPATEPRLLIALWFALPLALTLGCLAITRMEVHPYARWASPAGQRLVGALVRQADG
                     TADDRTFLTSVAVRGVHAIGEPDLRAAFAHREKYAGDRWERRA"
     gene            7348..8949
                     /locus_tag="APK71_RS22455"
     CDS             7348..8949
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (110-484). E-value:
                     2.1e-18. Score: 58.8. Matches aSDomain:
                     nrpspksdomains_APK71_RS22455_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972878.1"
                     /locus_tag="APK71_RS22455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_055468897.1"
                     /sec_met_domain="PF00561 (E-value: 1.7e-22, bitscore: 74.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYSAAGSLLLTTLTAVPAQAAPAAGPGSAEARGTAVAAGR
                     AAAAGIEFGACPRAEELPDGMRCGTVAVPLDYARPDGERIELTVSRVRATRKDPDDAGR
                     TVPGQGALVYNPGGPGASGLYFPLVGMVPEWKRLAAAYDLVGYAPRGVAPSAPLSCQDP
                     GDFFKGPRPAPARPSEAYKKERAAEAEAYARGCAERGGDALRHYHSLNNARDLEVVRAA
                     LGERRLTFMGGSYGTYLGALYAELFPSHVRRMVFDAVVNPDPEQVWYRNNLDQSEAFED
                     RWEDFKKWVAGHDDVYGLGDRPRKVQDAYEEAGARLAAEPAGGTVGPAQLQGAMLKAGY
                     YDDYWPRRAEALSAYLKGDPQPLIDQAGTRTEEGAVAAENSNAVYTAVECNDAPWPTDF
                     GVWDRDNTRLARRAPFETWDNVWTNLPCAYWRAPRQQPLDVRTAPGELPPTLILAAERD
                     AATPYEGALELRRRLAGSVLVTERDAGTHGVAAGPNACVNAHLEAYLLDGRLPARDASC
                     APRPEPEPRQAAAPRA"
     aSDomain        7678..8799
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_APK71_RS22455_Abhydrolase_1.1"
                     /evalue="2.10E-18"
                     /label="APK71_RS22455_Abhydrolase_1.1"
                     /locus_tag="APK71_RS22455"
                     /protein_end="484"
                     /protein_start="110"
                     /score="58.8"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASGLYFPLVGMVPEWKRLAAAYDLVGYAPRGVAPS
                     APLSCQDPGDFFKGPRPAPARPSEAYKKERAAEAEAYARGCAERGGDALRHYHSLNNAR
                     DLEVVRAALGERRLTFMGGSYGTYLGALYAELFPSHVRRMVFDAVVNPDPEQVWYRNNL
                     DQSEAFEDRWEDFKKWVAGHDDVYGLGDRPRKVQDAYEEAGARLAAEPAGGTVGPAQLQ
                     GAMLKAGYYDDYWPRRAEALSAYLKGDPQPLIDQAGTRTEEGAVAAENSNAVYTAVECN
                     DAPWPTDFGVWDRDNTRLARRAPFETWDNVWTNLPCAYWRAPRQQPLDVRTAPGELPPT
                     LILAAERDAATPYEGALELRRRLAGSVLVTERDA"
     gene            complement(8950..9681)
                     /gene="hemQ"
                     /locus_tag="APK71_RS22460"
     CDS             complement(8950..9681)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="APK71_RS22460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_030404097.1"
                     /transl_table=11
                     /translation="MSDDASTPAAERIPNKGKLAKDLNEVIRYTLWSVFKLKDTLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDLSGLRADADLMIWWHAETADQLQEAYNLFRRTK
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYISVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRGSEARRHVR
                     EEIPFFTGRRKDIGELVAGLA"
     gene            complement(9687..11171)
                     /gene="hemG"
                     /locus_tag="APK71_RS22465"
     CDS             complement(9687..11171)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445289.1"
                     /locus_tag="APK71_RS22465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_055468898.1"
                     /transl_table=11
                     /translation="MSASETPAGDVPPDTGPGHVVVVGAGIAGLAAAHRLLEGGARVTV
                     LEASDRVGGKLLPGEIAGVRVDLGAESMLARRPEAVGLARAAGLADRLQPPSTATASLW
                     TRGALRPMPKGHVMGVPGTAAALSGVLSEEGLARIGRDADLPRTEVGDDVAVGEYVAAR
                     LGREVVDRLVEPLLGGVYAGDAYCISLRSAVPQLFEAARTHTSLTEAVRALQGRTATSP
                     PSGPVFMGIEGGIGTLPPAVAASVRARGGEILTGAPVTELRRTASDGWRIVAGDRVLHA
                     GAVVVAVPAGPAAELLRAEAPAAAAELSAVEYASMALITLAYRRSDAAALPEGSGFLVP
                     PVDGRTIKAATFASRKWGWIADEDPDVVVLRTSVGRYGDTAVLGRDDEGLVAVARHDLG
                     EATGLTAEPVATRVTRWRDGLPQYPVGHHARVARLREHVAKLPGLAVCGAAYDGVGIPA
                     SVASAYAAADQIRGDLRGVEQLTAHPVQSLHGGAGE"
ORIGIN
        1 ccctgggcca cagcggctac ggccccctcg tccccgaccc cgacggtctg ctcgacctgc
       61 ccaagggctt ccgctaccgg gtgctgtccc gtgagggcga cgaactgcgc tccggcgagg
      121 gaaaggtgcc gtccaaccac gacggcatgt cggccttccc cggcagacac ggccgcaccc
      181 acctcgtccg caaccacgag aaccgcgcca acggccgcgt ccccgtgccc accgtcaagg
      241 gcctgacgta cgacccggag ggcaagggcg gctgtacggc gctggagctg gactcgcgca
      301 accacgtcct gtccgagcgg gtcgcgatcg ccggtaccgc cgtgaactgc gcgggcgggc
      361 ccacgccctg gcacacgtgg ctgacctgcg aggagaccga ggaccgggcg ggcaccaacg
      421 gctacaccaa ggaccacggc ttcatcttcg aggtcgaccc ggtcgagccg caccgcaccg
      481 gcgcggtgcc gctgaccgcg atggggcgct tccagcacga ggcgatcgcc gtggacccgc
      541 ggcgcggggt cgtctacgag acggaggacg cgttcgaacg accgttcggt ctcttctacc
      601 ggttcctgcc ggagaagccg ctgggcggcc ggggctcgct gcgggcgggc ggccggctcc
      661 aggcgatgcg ggtgcccggt gtacccgacc tgtcctcgat ccaggagacg ggcgccacct
      721 tcgaccgcat cgagtgggtg gacgtgcccg acccgctggc cgacgggacg ccgatccgct
      781 tccaggactt cggccggggc ggcatcaccc acgcgcagaa gctggagggc tgctactggg
      841 gcgggcagag cgtgtacttc gtgtcgtcct tcgcccacag cgacgagggc tcggccgccg
      901 accactacgg gcagatctgg cgctacgacc ccgagcggcg ccggctcacc ctggtgatcg
      961 tcttcggtcc ggacaccgac gtgcagttgc cgggcgagtc cccggacaac atctgcctgg
     1021 cgcccagcgg cggcctgatg gtctgcgagg acggcaacgg cgcgcagcac gtcttcggcg
     1081 tgacccggcg cggggaggtg tacgccatgg cccgcggcgc ccagaacatc gggacggagg
     1141 aggaacccga gtggggcgag ttcgccggcg tcaccttctc ccccgacggc gacacgatgt
     1201 acgtcaactg ctacaccccc ggcacgacct tcgccgtgac ggggccctgg cgcaggtgac
     1261 ggcgggacac cggcggggcg cgccgtccgg ccactgaagc ccccacccct gggtactcgg
     1321 gccgcatgac tcagaacgtg caggtcaacg gctcgtactg gcttcagacg gcaccgggcg
     1381 gcgcgccgcg tcccgcgctc ggcgaggatc tcgacgtcga cgtcgccgtg atcggtgcgg
     1441 gcgtcgcggg gctcagcgcg gcctgggaac tggtgcgggc cgggcggagc gtggcggtcc
     1501 tggaggccgg gcgggtcgcc gccggcgtca ccgggtacac cagcgccaag ctgaccgtcc
     1561 agcacaccct ggtctacgac aggctgcgcc gcacccgcgg tcccgagggc gcccggctgt
     1621 acgcccgctc gcagtccgag gcgatcgagc gcgccgccgg gatcgtcgcc gagctgggcg
     1681 tcgactgcga gtgggagacg cgcgacgcgt acacgtacgt ccgcgatccg ggccgcgtcg
     1741 acgaggtgcg ggccgaggcg gccgccgcga aggaggcggg gctgccggcg tccttcgtga
     1801 cggagaccgg gctgcccttc ccggtggcgg gcgccgtgcg ggtgaccggt caggcccagt
     1861 tccacccgct gaagtacctg cgggcgctcg ccgcggacct ggaggcgcac ggcgggcgga
     1921 tcttcgagga caccaccgtc cagggcctgg aggagggcga gccgtgccgg gtgaggacgg
     1981 aggcgggggc gacggtcacc gcccgggacg tcgtggtcgc cacccactac ccggtcttcg
     2041 accgcgccct gctcttcgcc cgtctctccc cccgccgcga gctggtggtc gccgggagca
     2101 tcggggcgga ccgcgacgtg gacggcatgt acatcacgcc ggaggagaac acccgctcgg
     2161 tgcgcaccgc gcccctggac gacggccgcc gcctgctcgt ggtcaccggg gagcacttca
     2221 cgccgggcac gggcgacacc cgggagcgct tcgggaccct ggccgcctgg gcggacgagc
     2281 acttccccgg cgtggagcgc acccacgcct gggccacgca ggacatcgac cccacggaca
     2341 ccgtgccgat ggtgggcccg atgcacccgg gcgcgcacca cacgtacgtc gccaccggct
     2401 tcggcggctg gggactgagc ggcggcatga tggcgggccg gctgctgacg gcgcagatca
     2461 ccggcgagga gtgcgcgtgg agcgggctgt acgacccgcg ccggctgcgc acggcggtgc
     2521 gcgaggcgcc ggcgctgctc aagacgcagg cggaggtggc ccggcacttc gtcggcgacc
     2581 ggctgcggcc cgtgccgccg gtggacgcgc tgccgccggg cgagggcgcg gtggtccggg
     2641 cgggcggtga ccggctcgcg gtctaccggg acgaggcggg cacgctgcac gcggtctcgg
     2701 cgcgctgcac ccacctgggc tgcctggtcg gcttcaacgc ggccgagcgc gcctgggagt
     2761 gcccctgtca cggctcccgc ttcgacacgg acggcaaggt gatcgagggc ccggcgacga
     2821 agccgctgga gcagcgggac atctgacggc ggccgggttc tggtccgggc gggtgaccgg
     2881 ggcggcaggt cggcccgcac ggtcatacaa accgaacatc ccacttctac ggtcgtacgg
     2941 tgatcactct tgtacgacgc gccaccgcgc tctgtgtcct gggcgccgcc ctcaccggct
     3001 gcggaaccgc cgagaccccg caggcccccc ggccggcgcc gtccgcgccg tccgcaccgt
     3061 cctcgccgtc cgccaccgcc tcgaagccgg ccccctccgg gcctccgaca ctcgcccccg
     3121 gcccggcggg cctgacgccc gtcttcgagc acggcccccg cacggacgcc gcgaaggacg
     3181 gcaagctggt cgccctcacc ttcgacgccg acatgaccgc cgaccagggg ccgcgggcgg
     3241 cggcggggga acggttcgac aatcccgcgc tgatcgcgac gctgcggaag ctgaaggtgc
     3301 ccgccacggt cttcatgacc ggccggtggg ccgaggagta ccccgacgag gcccgctcca
     3361 tcgggcggga cccgcagttc gagatcgcca accactccta cagccactac gcctacaccg
     3421 aggactgcta cggcctgccg acggtctcca aggacgacat gcgcgcggac ctggagcggg
     3481 cgtacaccgc gttcgagaag gccggggtgg cggacccgat gccctacttc cgcttccccg
     3541 gcggctgcta cgacgacgcg gtccgcaagg agctgtcggc caccggcgtc accgccgtgc
     3601 agtgggacgt ggtgagcggg gacgccttcg cgaccgacgc cgacgcggtg acccggcagg
     3661 tcctcgacgg ggtgcggccg ggttcggtcg tcgtcatgca ctgcacccgc agcgccgccc
     3721 cggtgaccga gcaggtggtg cgggcggtgg tgcccgaact gcgccgccag ggctaccgct
     3781 tcgccaaggt ctccgagctg atcggcgccg ccgacgggca cggctgacgt cctacgctgg
     3841 aggcatgagc agcgaccacg accccgcccc ggacggctcc ggctccggct gcgaccccgg
     3901 cggatattcc ctgatcggca cggcgcccca cccgcccgtg gccgacggcc cgccgtacgc
     3961 ggagtgcgtc ctgtgccggg agccgacgga gtaccccgag tcgtacaagg gcatcacgct
     4021 ctgcccggtc tgcgagtggc aggaggcgca gcgcacggcc tgcacgggct gaccggtcgc
     4081 ctcgcgcgcg gtacggggcc gtgcgcccgc gagcggcggg cttggcagac tggagggcgt
     4141 gagcaacgac ccgaccgcac ccgcccccgt tcccgtcccg gacggcccct tccggcccga
     4201 gcccggcgac cgcgacctcg cgccgcagtt cgtgctgccg ctcgtcgtgc gcatcgagcg
     4261 ggcggctccc ccggcccgta ccgacgccct ggagaccgcg gcccgggcgg tcctggtgat
     4321 gctcgccgac gcgcggtcga ccggtgacgg cgcgtgggcg cgggcgatgc gggactggca
     4381 ggacgcgcgc atccgcaagg tcgtcaggcg ggctcggggc gcggagtggc gccgggccgg
     4441 ggcgctgccc ggcgtcacgg tcaccggcaa gagcgccgag gtgcgggtct tcccgccggt
     4501 cccgctggac ggctggccca aggacctggc ccgtctccag gtctccggca ccgacctcga
     4561 cgaccccgag ccgccggccg gcgccgaccc ctgcgcgccg gtgctctggc tgaaccccgg
     4621 gctggacatg tcggcgggca aggcgatggc gcaggccggc cacggcgcgc agctcgcctg
     4681 gtgggagctg tcggacggcg agcgggcggc gtggcgcgac gcgggcttcc cgctgtcggt
     4741 gcggaccgcg gacccggccc gctggccgga cctcaccgcc ggcggtctgc cggtggtgcg
     4801 cgacgccggg ttcacggaga tcgcgccggg ctcgtgcacg gtggtggcgg accacccggc
     4861 cctgcggacc ccgccgggcg gggcgccggg ggcctgaacc tcaagatttg ctctgaaggc
     4921 gtggcccgcg cggttcgcgg ccggtgtccg gggcgataac ccgtcggagc gacggggagg
     4981 ggcggacgag catggagcca atcacgcggc tgggcaccgg catcggatgg cggccggaga
     5041 tcgcggacgt cgtcgagcgg atgcccggca tcgactgggt cgaggccgtg tcggagaacc
     5101 tgtgtcccgg gcatctgccc gactcgctgc tgcggctgcg cgagcgcggg gtgaccgtcg
     5161 tgccgcacgg cgtctccctc gggctcggcg gcgccggccg gccggatccg gggcgcctgg
     5221 cggcgctcgc cgagcgggcg gaggtcctgg catcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtgcgggc gggcggcgcg ccgaccgcct cgccggtgct ggaggcgggg cacctgctgc
     5341 cggtgccgcg cacccgggac gccctcgacg tgctgtgcga gaacgtgcgc atcgcgcagg
     5401 acgcgctgcc cgtgccgctg gccgtggaga acatcgccgc gctcgtcgcc tggccgggcg
     5461 aggagctgac ggaggggcag ttcctgtacg aactggcgga ccggaccggc gtacggctgc
     5521 tgatcgacgt ggccaacctg cacaccaacc acgtcaacct cggcgcggac ccggccgagg
     5581 cgctcgccga actgcccctg gaggccctcg cctacgtcca tgtcgcgggc ggcttcgaac
     5641 gggacggcgt ctggcacgac agtcacgccc acccggttcc gcggccggtc ctcgacatcc
     5701 tgacggacct cgccgcacgg gtcgcgccgc cgggcgtcct gctggagcgg gacgagaact
     5761 tccccgaggg cgacgagttg cgcgccgagg tggaggcgat ccgcgacgcc gtggagcggg
     5821 gccggaccct gcgcgggccg gcttccgggg gcggggcggc gccgcgcacg ccgggcccgg
     5881 gttccgtccc ggccgccggc gacgccgtac ggcagcggct cgggctcgcg caggccgcgt
     5941 tgttgtccgc gctggtcgcg ggggcgccgg tgcccgaggg gttcgacccg gtgcggctcg
     6001 gggtgcaggc gcgtgcgctc gccggcaagc gggccgacgt ggtcggcaag gtcgcgcccg
     6061 aactgcccgt gatcctgggc ccgcggtacc ggccggcctt cctcgcctac gcgcgggggc
     6121 gcccgatgac cggcggctac cgtcgcgacg ccctcgactt cgtcgcgcac ctgctgcgca
     6181 cggcaccggg tgacgatccc ggcgtgcggc gggcgttgcg cgcgtggtgg ctggagcggt
     6241 cggggccggt gccccgctcg tcgcgtcccg gggtccgact ggcccgtgcg gccaggcgcg
     6301 tgctgctgcg ccgctgaacg gagcggtccc gggggacacc gccgaacgga acgtcacata
     6361 cccgcaacac cgcgtcccgg aatgtgaaca gggcatggcg ccggcgaaac cttttggcat
     6421 agaagtacgg catgttctgg gtcctcttcc tcctggcggc gtgggccgtc gccggtgtcg
     6481 cgtgcctgcg gctgtgcctg gccgcggtgc gcgcggcggc cgtcgacccg cacgccgtcg
     6541 tccgtgagca cacgctgacg ctgtacgagg cggcgttcct gtccggcggt ccgcggcggg
     6601 tggccgacct gacgctcgtc tccatggcgc gccagcgacg gctgctgctc gcgcacaccg
     6661 gctgggcgac cgtcgtggac ccctgcggcc gggacgacat ggagcggtcg gtcatagggg
     6721 ccatagggcc gcaagggcag tcccggatag cgccggtgcg gaccgcggcc gccacggccg
     6781 acgcggtacg gggcctggcc gaccggctgg tcggagcggg cctggccctg cccgacggcg
     6841 gtaccggcgg cgtcgcggcc ggggtgcgcc gggtgcgggc cgccgcggcg gccgtgtgcg
     6901 cgctcggcct ggtctccctg gccctgcccc tgcccgccac cgagccgcgc ctgctcatcg
     6961 ccctctggtt cgcgctgccg ctcgccctga ccctcggctg cctggccatc acgcgcatgg
     7021 aggtccaccc gtacgcgcgc tgggcctccc cggccggaca gcgactggtc ggcgccctcg
     7081 tccggcaggc cgacggcacg gccgacgacc ggaccttcct cacctcggtc gccgtgcggg
     7141 gcgtccacgc gatcggcgag ccggacctgc gtgcggcctt cgcccaccgg gagaagtacg
     7201 ccggggaccg ctgggagcgc cgggcctgac gcgccggggg cgtacggggg cactcggcgc
     7261 cgacatgcgc cggcggtgct tgccccgccc ggccggtgaa ccaaccatcc cttgtgtcac
     7321 tcccccgtgc caccgaaggg atacgcgatg cgagctgccg ccctctactc ggccgccggg
     7381 tccttgctgc tgaccaccct caccgccgtc ccggcgcagg cggcgcccgc ggccgggccc
     7441 gggtccgccg aggcgcgcgg caccgccgtg gccgccgggc gcgcggcggc ggccgggatc
     7501 gagttcgggg cgtgcccgag ggcggaggag ctgccggacg gcatgcgctg cggcacggtc
     7561 gccgtgcccc tggactacgc ccgtcccgac ggcgagcgga tcgagctgac cgtcagccgc
     7621 gtgcgggcca cccgcaagga cccggacgac gccgggcgca cggtccccgg acagggcgcc
     7681 ctcgtctaca acccgggcgg ccccggcgcc tccgggctgt acttcccgct ggtcggcatg
     7741 gtgcccgagt ggaagcggct cgcggccgcc tacgacctcg tcggctacgc cccgcgcggg
     7801 gtcgcgccct ccgcgccgct gtcctgccag gaccccggcg acttcttcaa gggaccgcgg
     7861 cccgcaccgg cgcggccctc ggaggcgtac aagaaggagc gcgccgccga ggcggaggcg
     7921 tacgcccgcg gctgcgcgga gcggggcggg gacgcgctgc ggcactacca ctcgctcaac
     7981 aacgcccgcg acctggaggt cgtccgcgcc gcgctgggcg agcggcggct caccttcatg
     8041 ggcgggtcgt acggcaccta cctcggcgcg ctgtacgccg agctgttccc ctcgcacgta
     8101 cggcggatgg tgttcgacgc ggtggtgaac ccggacccgg agcaggtctg gtaccgcaac
     8161 aacctcgacc agtccgaggc gttcgaggac cgctgggagg acttcaagaa gtgggtcgcc
     8221 gggcacgacg acgtgtacgg gctcggggac cggccgcgga aggtgcagga cgcctacgag
     8281 gaggcgggcg cgcgactggc ggccgaaccg gcgggcggga cggtggggcc cgcccagttg
     8341 cagggcgcga tgctgaaggc cgggtactac gacgactact ggccccgccg cgccgaggcg
     8401 ctgtcggcgt acctgaaggg cgatccgcag ccgctgatcg accaggcggg gacgcggacc
     8461 gaggagggcg ccgtcgcggc ggagaactcc aacgccgtgt acacggccgt cgagtgcaac
     8521 gacgcgccct ggcccaccga cttcggggtc tgggaccggg acaacacgcg gctggcccgc
     8581 cgggcgccgt tcgagacgtg ggacaacgtg tggaccaacc tgccgtgcgc ctactggcgg
     8641 gcgccccggc agcagccgct cgacgtgcgc accgcaccgg gtgagctgcc gccgacgctg
     8701 atcctggccg ccgagcggga cgcggcgacg ccgtacgagg gcgcgctgga gctgcggcgg
     8761 cggctggcgg gctcggtgct ggtgaccgag cgggacgcgg ggacgcacgg cgtcgccgcc
     8821 ggcccgaacg cctgcgtcaa cgcccacctg gaggcgtacc tgctggacgg ccgcctcccg
     8881 gcgcgggacg cgtcctgcgc cccgcgcccg gaaccggagc cgaggcaggc cgcggcgccg
     8941 agggcctgac taggccagcc cggcgaccag ctcgccgatg tccttgcggc gcccggtgaa
     9001 gaacgggatc tcctcgcgga cgtggcggcg ggcctcggag ccccgcaggt ggcgcatgag
     9061 gtcgacgatg cggtgcagtt cgtcggcctc gaaggcgagg atccactcgt agtcgcccag
     9121 cgagaaggag gcgaccgtgt tggcgcgcac gtccgggtag ccgcgggcca tcttgccgtg
     9181 gtcggcgagc atgcggcggc ggtcctcgtc gggcagcagg taccagtcgt acgagcgcac
     9241 gaacgggtag acgctgatgt agttccgcgg cgtctcgtcg gccaggaagg ccgggatgtg
     9301 cgagcggttg aactcggcgg ggcggtgcag cgccatgttc gaccagaccg gctccagcgc
     9361 acggccgagc ttcgtgcggc ggaagaggtt gtacgcctcc tggagctggt cggcggtctc
     9421 ggcgtgccac cagatcatca gatcggcgtc ggcccgcaga ccggacaggt cgtaggtgcc
     9481 gcggatcgtc acgtccttcg cggcgagctg gtcgaacagc tcctggacct cgtcggcgta
     9541 cccggcgcgg tcctcgggga gggtgtcctt gagcttgaag acggaccaga gggtgtagcg
     9601 gatgacctcg ttgaggtcct tggccagctt gcccttgttg gggatccgct cggcggcggg
     9661 ggtggaggcg tcgtcactca tggtccctat tctcccgctc cgccgtgcag actctgcacc
     9721 gggtgggccg tgagctgctc cacaccgcgc aggtcgcccc ggatctggtc ggcggccgcg
     9781 tacgcgctcg cgacgctcgc cgggatgccg acgccgtcgt acgccgctcc gcacaccgcg
     9841 agcccgggga gtttcgcgac gtgctcgcgc aggcgggcca cgcgcgcgtg gtggccgacg
     9901 gggtactgcg gcaggccgtc ccgccagcgg gtgacgcggg tggcgacggg ctcggcggtc
     9961 aggccggtgg cctcgcccag gtcgtgccgg gcgacggcga ccaggccctc gtcgtcccgg
    10021 ccgaggaccg ccgtgtcgcc gtagcggccc accgaggtgc gcaggacgac cacgtccggg
    10081 tcctcgtcgg cgatccagcc ccacttgcgg gaggcgaagg tggccgcctt gatggtgcgc
    10141 ccgtcgaccg ggggcacgag gaagccgctg ccctcgggga gcgccgcggc gtcggagcgg
    10201 cggtaggcga gggtgatcag ggccatcgag gcgtactcga cggcggacag ctcggccgcg
    10261 gcggcgggcg cctcggcgcg cagcagctcc gccgcgggcc cggccgggac ggcgacgacg
    10321 accgcgccgg cgtgcaggac ccggtcgccg gcgacgatcc gccagccgtc ggacgcggtg
    10381 cggcgcaact cggtgacggg cgccccggtg aggatctcgc cgccgcgcgc gcggaccgac
    10441 gcggcgaccg cgggcggaag ggtgccgatg ccgccctcga tgcccatgaa cacggggccg
    10501 ctcgggggtg aggtggccgt gcggccctgg agggcgcgga ccgcctcggt gagggaggtg
    10561 tgggtgcggg cggcctcgaa gagctggggc acggccgaac gcagggagat gcagtaggcg
    10621 tcgcccgcgt agacaccgcc caggagcggc tcgacgaggc ggtcgacgac ctcgcggccc
    10681 aggcgcgcgg cgacgtactc ccccacggcc acgtcgtcgc cgacctcggt gcggggcagg
    10741 tcggcgtcgc gcccgatgcg ggcgaggccc tcctcggaga gcacgccgga cagggcggcg
    10801 gcggtgccgg ggacgcccat gacgtggccc ttgggcatgg ggcgcagggc tccgcgggtc
    10861 cacagggagg ccgtcgcggt ggacggcggc tggaggcggt cggcgagccc ggcggcgcgg
    10921 gccaggccga cggcctcggg gcggcgggcc agcatcgact cggcaccgag gtcgacgcgt
    10981 acgccggcga tctcgccggg cagcagcttg ccgccgacac ggtcggacgc ctccaggacg
    11041 gtcacccgcg caccgccctc caggaggcgg tgggcggcgg ccagtccggc gatgccggct
    11101 ccgacgacga cgacgtgtcc gggtcccgtg tccgggggga cgtcccccgc cggagtctcg
    11161 cttgcgctca tacccccagc gtctcagacc ccacctacac cgggcccggg ggccgtcggc
    11221 tgcgacaccg ggcgcgggtg gcgccgcccc ccgacacggg ctcacgggtg ccgcgccggc
    11281 ccccgcgcgg cacccgtgag gcgcaccggc acgcgca
//
