LOCUS       NZ_BBOK01000007        10216 bp    DNA     linear   CON 17-AUG-2024
DEFINITION  Streptomyces sp. NBRC 110030, whole genome shotgun sequence.
ACCESSION   NZ_BBOK01000007
VERSION     NZ_BBOK01000007.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. NBRC 110030
  ORGANISM  Streptomyces sp. NBRC 110030
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Katano-Makiyama,Y., Hosoyama,A., Hashimoto,M., Tsuchikane,K.,
            Uohara,A., Kitahashi,Y., Ohji,S., Ichikawa,N., Kimura,A.,
            Yamazoe,A., Komaki,H., Igarashi,Y. and Fujita,N.
  TITLE     Whole genome shotgun sequence of Streptomyces sp. NBRC 110030
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 362051)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 97x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417675.1-RS_2024_08_17
            Annotation Date                   :: 08/17/2024 02:44:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,152
            Genes (coding)                    :: 7,019
            CDSs (with protein)               :: 7,019
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 45 of 133
            Pseudo Genes (incomplete)         :: 107 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 23 of 133
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:33:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3880
            Orig. end                         :: 14096
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBOK01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(93..2558)
                     /locus_tag="APK71_RS18265"
     CDS             complement(93..2558)
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003995665.1"
                     /locus_tag="APK71_RS18265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_031085304.1"
                     /transl_table=11
                     /translation="MGDVVDEGTAERGSRVLRAEGALRAVAAGPAPRDRQRRFLEQALV
                     FAGASFAALYMPGDDRELLCLAEAVGVPRTLYGVRDSYPASGGSPVADARRAGEPVWIG
                     PEEFAGSAESRHVPSSGFSLAVLPVRGDGGGCLFALTEHPGGFDAEDRACLEAIAEAMT
                     CPAPAGTPEPGVLRPDAFTLAMDTGRAEVGGAVLRLFGLTPEEFDGKVETLLGLTVPED
                     LPSLMSVTESDHMTIGERELEFRVLQPTGPPKWLRLSGRLLPGGDGRPARLVGTVADAS
                     TLRPDVTDVARVQRLAAALATAGTVREVSQAVVAALRRPLRADRIALAELEHDRLVVTV
                     LDPPDSESWPELWRLEWRTEWPDAPVRAMPTLAAALREGRARIWPAGTGDLEPALADVG
                     PGGLAVLPLPAGGRMAGACLIGWDAPHDFGPDERALLTASAGLAGQALMRAHAFDAEHE
                     LVGMLQRQLLPRRLPKLPGAVAVARYLPSTAGLELGGDWYDVIPLPDHHVALVIGDVQG
                     HSTGAATLMGQMRTALRAYAVEGHPPDVVVSHANRLLTGMGTDLFATCVYVDLDMEEGS
                     AWCVRAGHLPPVLRHPDGSTEIAEAEGGPPLGVVTQADFPMSPLRLRPGTLIALTTDGL
                     VESPDSDIDEGMGRFAHALAVSDPAHLGLVADALLGNARRNDDVALLLVRYDGMALRPL
                     RESWSVWRLPEAVRHARRFTRRTLRSWGVPGDDVDGVLLVVSELVTNALVHTDGQVRLD
                     LTLIGGRLRVAVADASPRTPARPTGIGWEATGGRGILLVEAMSAAWGTLPVGGGKQVWS
                     EMSVGVHTP"
     gene            complement(3074..4339)
                     /locus_tag="APK71_RS18270"
     CDS             complement(3074..4339)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978109.1"
                     /locus_tag="APK71_RS18270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S1 family peptidase"
                     /protein_id="WP_055468497.1"
                     /transl_table=11
                     /translation="MSHKRIPKRRAAMAAGGVIALGAAALLLPHANAAQDGGSPDEAAA
                     PRTLKAADAPDLAARLAGLLGDTFAGSYYDSGARRLVVNVVPGDDDEAVVRAKRAGAEV
                     REVDNSMGELRSGARTLKSEASIPGTSWAIDPRTNRILVTADSTVTGDRWDRLESTVEG
                     LGSGMATIRRSAGTFTTFVSGGDAVFGGGARCSLGFNVTAGDGSPAFLTAGHCGVAAAQ
                     WSDAQGGRPIATVDRAVFPGQGDFALVRYDDPATDAPSEVNLGDQTLPISDAAEATVGQ
                     EVFRMGSTTGLADGRVLGLDATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTRDGLAIGL
                     TSGGSGDCTTGGETFFQPVTTALNAVGATLGDEGAGAGAGEAADDGEASAGGGGGGGEG
                     AGAGAGHAGAGGVAGDGSGAGE"
     misc_feature    4535..4548
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.1"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="APK71_RS18275"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="APK71_RS18275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030399642.1"
                     /sec_met_domain="strepbact (E-value: 2.1e-38, bitscore:
                     125.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEISDADLDNVSGGLNVNAVGTVTGLVDGVAPVSGL
                     LNTAVGTVEGVTGLNTAPVTNLVAGL"
     gene            5319..6125
                     /locus_tag="APK71_RS18280"
     CDS             5319..6125
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="APK71_RS18280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_030399641.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPDELDLPVRFARPQGWLVLSVTVVAMAAASVW
                     AVSGSVTSTVDAPAVLTHGQGSYLLQSPVSGQVTAVLARPGQRLAADAPVLKVRTSEGE
                     SVVRTVDAGRVSALAATVGQIIRTGANVAAVEKVAHADDPLYATVYVPAENAAAIPAHA
                     SVDLTVQSVPAQRYGVLHGEVKSVDRSAQSAQTIGAFLGDDALGEQFTKDGRPVAVTVR
                     LETSASTKSGYAWSSAEGPPFGLTSMTLASGSIRLADQRPVDWLLP"
     gene            6122..8353
                     /locus_tag="APK71_RS18285"
     CDS             6122..8353
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="APK71_RS18285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_030399640.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.2e-40, bitscore:
                     131.5, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQETRETRGRRRAAPPRRGVPKSRAKTVRTPTVLQMEAVECG
                     AASLAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYGLTAKGMQMDLAALAE
                     VAAPAILFWEFNHYVVYDGMGRRFGRRGVFVNDPGKGRRFVSLEEFDGSFTGVVLTMEP
                     GEDFTRGGRRPGVLGAMPARLRGTAGTLPAALLASLLLVAVGAAVPALSRTYIDMFLIG
                     GQTSLLGVLFASMGACVLLTVVLTWLQQANLLHGRIISSTLSSARFLRHLLRLPVTFFS
                     QRSPADLVQRLQSNDAVAETLARDLAAAGVDAIVVVLYAVLLYTYDPQLTFVGIGVALL
                     NIVAMRVVIRLRATRTAKLRADTARLTNTAYTGLQLIETMKATGGEDGYFRKWAGQHAT
                     TLEEQQRLGVPSAWLGVVAPTLATLNSALILWIGGMRAVEGHISVGLLVAFQALVVRFT
                     APLTRLNGVAGRIQDFAADVARLKDVENFRADPLYDRPGGTGSTRRLHGHVELQNVSFG
                     YNPLDKPLLSGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYAPWDGVIRVDGQRLDD
                     IPRGALASSVSFVDQDVFLFEGSVRDNVALWDPSIPDDAVVEALKDAALYDVVTRRPGG
                     IHSPVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELVVMDNLRRRGC
                     ACVVIAHRLSTVRDSDEIVVLEHGTVVERGRHAELVAHGGAYAALVGER"
     misc_feature    complement(9027..9041)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
ORIGIN
        1 gcctcttgcg cgtcctgcgc ccggtggcgc tcgcccgtcc gggtcccctc gcggggcgcc
       61 gtgaaacccg ggacgcgggg gccgcgaggg gcctacgggg tgtgcacgcc cacggacatc
      121 tcgctccaca cctgcttgcc cccgccgacc gggagcgtcc cccaggccgc cgacatggcc
      181 tcgaccagga ggatgccgcg tccgcccgtg gcctcccagc cgatgccggt cggccgggcc
      241 ggcgtacgcg gggaggcgtc ggcgaccgcg acgcgcagcc ggccgccgat cagggtgagg
      301 tcgagccgca cctgtccgtc ggtgtgcacg agggcgttgg tgaccagctc ggagacgacg
      361 agcaggacgc cgtcgacgtc gtcgccgggg acgccccagg aacgcagcgt ccgccgggtg
      421 aagcggcggg cgtgccgcac ggcctcgggc agccgccaca ccgaccagct ctcccgcagc
      481 gggcgcagcg ccatgccgtc gtagcgcacg agcagcaggg cgacgtcgtc gttgcggcgg
      541 gcgttgccga gcagggcgtc ggcgacgagt ccgaggtggg cggggtcgga gacggccagc
      601 gcgtgcgcga agcggcccat gccctcgtcg atgtcggagt cgggcgactc cacgaggccg
      661 tccgtggtga gggcgatcag cgtgccgggg cgcagccgca gcgggctcat cgggaagtcg
      721 gcctgcgtca ccacgccgag cggcgggccg ccctcggcct ccgcgatctc ggtgctgccg
      781 tcgggatggc gcagcaccgg cggcaggtgc cccgcccgca cgcaccacgc ggagccctcc
      841 tccatgtcga ggtcgacgta gacgcaggtg gcgaagaggt cggtccccat gccggtcagc
      901 agccggttgg cgtgggagac caccacgtcc ggcgggtggc cctcgacggc gtaggcgcgc
      961 agggcggtgc gcatctggcc catgagggtg gccgcgccgg tgctgtggcc ctggacgtcg
     1021 ccgatgacga gggcgacgtg gtggtcgggc agcggaatca cgtcgtacca gtcgccgccc
     1081 agttcgagcc cggccgtgct gggcaggtag cgggcgacgg cgaccgcgcc gggcagcttg
     1141 ggcaggcggc gcggcaggag ctggcgctgg agcatgccga ccagctcgtg ctcggcgtcg
     1201 aaggcgtggg cgcgcatgag ggcctgcccc gcgaggcccg cggacgcggt gagcagggcg
     1261 cgctcgtcgg ggccgaagtc gtgcggggcg tcccagccga tgaggcaggc accggccatg
     1321 cggccgcccg cgggcagcgg cagtacggcc aggccgcccg ggcccacgtc ggcgagggcg
     1381 ggctccaggt cgccggtgcc ggcgggccag atccgggcgc gtccctcgcg cagtgcggcg
     1441 gcgagggtgg gcatggcccg taccggcgcg tcgggccact cggtgcgcca ttccaggcgc
     1501 cacagctcgg gccaggactc ggagtcgggg ggatcgagga cggtgacgac gagccggtcg
     1561 tgctccagct cggccagcgc gatccggtcg gcgcgcagcg gtctgcgcag ggcggcgacg
     1621 acggcctgac tgacctcgcg gacggtcccg gcggtggcca gcgcggcggc gagccgctgg
     1681 acgcgggcga cgtcggtgac gtccggacgc agggtggagg cgtcggcgac ggtgccgacc
     1741 aggcgggccg gccggccgtc cccgccgggc agcaggcgtc cgctgagccg gagccacttc
     1801 ggcggcccgg tgggctgcag cacccggaac tccagctcgc gctccccgat ggtcatgtgg
     1861 tcggactcgg tcaccgacat cagcgacggc aggtcctccg gcacggtgag gccgagcagc
     1921 gtctccacct tgccgtcgaa ctcctccggc gtgaggccga acaggcgcag cacggcgccg
     1981 ccgacctccg cacggccggt gtccatggcc agggtgaagg cgtccggccg gaggacgccg
     2041 ggctcggggg tcccggccgg ggcggggcag gtcatcgcct cggcgatcgc ctccaggcac
     2101 gcgcggtcct ccgcgtcgaa ccctccgggg tgctcggtca gggcgaagag gcagccgccg
     2161 ccgtccccgc gcacgggcag cacggccagc gagaagccgc tcgacggcac gtgccgcgac
     2221 tccgccgatc cggcgaactc ctccggcccg atccacaccg gctcgccggc acggcgggcg
     2281 tccgccaccg gggagccgcc ggacgcgggg tagctgtcgc gtacgccgta cagggtgcgc
     2341 ggcacaccga ccgcctcggc cagacacagc agctcacggt cgtccccggg catgtacagg
     2401 gcggcgaagg acgcgcccgc gaacaccagg gcctgttcga ggaaccggcg ctgccggtcc
     2461 cgcggcgccg gcccggccgc gaccgcccgc agggcgccct cggcacgcag caccctcgat
     2521 ccgcgttccg cagtgccctc gtcgaccacg tcgcccatta cagcgctaac gggacatctg
     2581 cgcagcccct gcgactcggg catcggcggg ccggacacac gccgggggcc cagggacggg
     2641 tgctgcgcgt cggggcggcg ggggcatggc gggtacgggc ggaggcgggc ctgccggtgg
     2701 gcgcgaagga gggcctaggg cacagggcac gcacctctgc gcaagggctg caccccaacc
     2761 gcaccgtggt cgtcggccgg cgacgctccg ggcctggggc gacggaccgg gcccggagca
     2821 cagccgagcg ggtgtgcagg gcgccgcgcc tgccccaccg gccaccgtgc ccgccggtac
     2881 ggagcgcgtc cgccgggcac ctgggacacc gccgggagcc ggcatcccgc cccgccccgc
     2941 cggaggcagg gccggtccca ctggggccgg tccgtcaccg gagggccggt ccaccggagg
     3001 gccgatccca ccgggggccg cccacccggg aggccgggtc ccaccagtgg gcggcggaca
     3061 cggcgtcagc ccgtcattcc ccggcccccg agccgtcccc ggccacccca ccggcacccg
     3121 cgtgccccgc accggcaccc gcgccctcac cgccaccgcc accgccaccg gcactcgcct
     3181 ccccgtcgtc ggccgcctcg cccgcgcccg ctcccgcgcc ctcgtccccg agggtcgcgc
     3241 cgaccgcgtt cagggccgtg gtgaccggct ggaagaaggt ctcgccgccg gtcgtgcagt
     3301 cgccgctgcc gccggaggtc aggccgatcg cgagaccgtc ccgggtgaag agggagccgc
     3361 cgctgtcgcc gggctcggcg cagacatcgg tctgtatgag gccggtgacg gtgccctctg
     3421 ggtagttgac ggtggcgtcc aggccgagca cccggccgtc ggccaggccg gtcgtactgc
     3481 ccatgcggaa gacctcctgg ccgaccgtcg cctcggcggc atcgctgatc ggcagggtct
     3541 ggtcgccgag gttcacctcg ctcggggcgt ccgtcgccgg gtcgtcgtac ctgacgagcg
     3601 cgaagtcgcc ctggccgggg aagaccgcgc ggtcgacggt ggcgatcggc cgtccgccct
     3661 gcgcgtcgga ccactgggcg gccgcgaccc cgcagtgacc ggccgtcagg aaggcgggcg
     3721 agccgtcgcc cgcggtgacg ttgaaaccga gcgagcaccg cgctccgcca ccgaagaccg
     3781 cgtcgccgcc cgacacgaag gtggtgaagg tgccggcgga cctcctgatc gtcgccatgc
     3841 cggagccgag cccctcgacg gtggactcca ggcggtccca ccggtcgccg gtgacggtgc
     3901 tgtcggcggt caccaggatc ctgttggtgc gcgggtcgat cgcccacgag gtgcccggga
     3961 tgctcgcctc ggacttcagg gtccgcgcgc cgctgcgcag ttcgcccatg ctgttgtcca
     4021 cctcgcggac ctccgcgccc gcccgcttcg cccgcacgac ggcttcgtcg tcgtccccgg
     4081 ggacgacgtt gacgacgagc cgccgcgcgc cggagtcgta gtaggagccg gcgaaggtgt
     4141 cgccgagcag gccggcgagg cgtgcggcga gatccggcgc gtcggcggcc ttgagggtcc
     4201 ggggcgccgc cgcctcgtcc ggcgagccgc cgtcctgcgc ggcgttggcg tgcgggagca
     4261 ggagggcggc cgcgccgagc gcgatcacgc cgcccgccgc catcgcggcc ctgcgcttgg
     4321 gaattcgctt gtgactcaac ctgctcgacc tcctgggacc ggggcccgtg cccaccggtc
     4381 cggcaactgt tgggggcgcc tggacggctg ttggtacgca cgggccgggc ggggcgttca
     4441 gccggaccgc cgggcccccg gggcgcccct cctcgcctcc ctcgaaagtc gcaccgcgac
     4501 gtcaggtccc gaagtcgcac cggacctacg tcgcttgaga ccgatcaggc accctccgcg
     4561 tccatccgga gacgcaacgt gacgggcggg gagcggaatg accgcttcag tgaatctgca
     4621 catcgaatgc tcaacccggt cacccccgtt ggagcatctg cacagcccgc gggggcccgt
     4681 acgcctttgg gcccggagtg tcccaatcgc ctgcgcgcgg gcaaggggcc gcacgagccg
     4741 atctcgcgca gcgtgtgaag aagtctgcgc gaaggggtgc gcactcctgc acgcttgggt
     4801 gcccgtccgt cgcaagtgcg ctggtcggag gggtgattga ttggaagccc gaaccggccg
     4861 cgtgctttga tccatcgcac cgcgggggcc gcggagagct cgggaaacgg gcgtccggcg
     4921 cctgcggccg cggccgtcat ctgtccccag agggggccgc tccccccttg tgaaatggct
     4981 atacgaaggg aagttccatc atgaactcca ccccccaggt cgagaccgtc gagatctccg
     5041 acgccgacct cgacaacgtc tccggcggcc tgaacgtgaa cgccgtcggc accgtcaccg
     5101 gcctggtgga cggcgtcgcc ccggtctcgg gcctgctcaa caccgccgtc ggcaccgtcg
     5161 agggtgtcac cggcctgaac acggccccgg tcaccaacct ggtcgccggt ctctgatcgc
     5221 gcccttgtgt cgctgagtcc cggagcccgc caggctccgg gactcttcgg ccgttgtcgt
     5281 tcttcggccg ttctcctcga gcccgggata cgtcagccgt gcagttccgc caacaggccc
     5341 tctccaaact ccagtcgccg gacgagctcg atctcccggt gcgtttcgcc cgtccccagg
     5401 gctggctggt gctgtccgtg accgtggtcg ccatggccgc cgcgtccgtg tgggccgtga
     5461 gcggctcggt gacctccacg gtcgacgccc ctgccgtcct cacccacggg cagggcagct
     5521 acctcctgca gagtcccgtc tcgggccagg tcaccgcggt cctggcgcgg ccgggacagc
     5581 ggctggccgc cgacgccccg gtgctgaagg tccgcacgtc cgagggcgag agcgtggtcc
     5641 gcacggtcga cgcgggccgg gtcagcgcgc tcgcggccac cgtcgggcag atcatccgga
     5701 ccggcgcgaa cgtcgccgcc gtcgagaagg tcgcccacgc cgacgacccc ctctacgcca
     5761 ccgtctacgt cccggccgag aacgccgccg ccatccccgc ccacgcctcc gtcgacctga
     5821 ccgtgcagtc ggtgccggcc cagcggtacg gcgtcctgca cggcgaggtg aagtccgtgg
     5881 accgctcggc ccagtcggcg cagacgatcg gcgccttcct cggggacgac gcgctcggcg
     5941 agcagttcac caaggacggc aggccggtcg ccgtcaccgt gcggctggag acctcggcgt
     6001 ccacgaagag cggctacgcg tggtcctccg ccgaggggcc gccgttcggg ctcacctcca
     6061 tgaccctggc ctccggctcg atccggctgg ccgaccagcg tcccgtcgat tggctgctgc
     6121 cgtgagcacc gcacaggaga cccgggagac ccgcggccgg cgccgtgccg ccccgccgag
     6181 gcgcggcgtg cccaagagcc gcgcgaagac cgtccgtacg cccaccgtgc tccagatgga
     6241 ggccgtggag tgcggcgcgg cctccctcgc gatggtgctc gggcactacg gccgccatgt
     6301 cccgctggag gagctgcgga tcgcctgcgg ggtctcccgc gacggttcgc gcgccagcaa
     6361 cctgctgaag gcggcccgga gttacgggct caccgccaag ggcatgcaga tggacctggc
     6421 cgcgctcgcc gaggtggcgg caccggcgat cctgttctgg gagttcaacc actacgtcgt
     6481 ctacgacggc atgggccgcc ggttcggacg gcgcggggtg ttcgtcaacg acccgggcaa
     6541 gggacgccgt ttcgtgtccc tggaggagtt cgacggcagc ttcaccggtg tcgtcctgac
     6601 catggagccc ggcgaggact tcacccgcgg cggtcgccgg cccggtgtgc tcggcgcgat
     6661 gccggcccgg ctgcgcggca ccgccggcac gctgcccgcc gccctgctgg cgagcctgct
     6721 cctggtggcg gtcggggcgg cggtgcccgc gctgagccgt acctacatcg acatgttcct
     6781 gatcgggggg cagacctcgc tgctcggcgt gctgttcgcg tcgatgggtg cctgtgtgct
     6841 gctcaccgtg gtgctgacct ggctccagca ggccaacctg ctgcacggcc gcatcatctc
     6901 ctcgaccctc tccagcgccc ggttcctgcg gcacctgctg cggctgccgg tgaccttctt
     6961 ctcccagcgc agccccgccg acctcgtgca gcggctgcag tccaacgacg ccgtcgccga
     7021 gacgctggcc cgcgacctcg ccgcggcggg cgtggacgcg atcgtggtcg tcctgtacgc
     7081 cgtgctgctg tacacctacg acccccagct caccttcgtc ggcatcgggg tggcgctgct
     7141 caacatcgtc gcgatgcggg tcgtgatccg gctgcgcgcg acccgtacgg cgaagctgcg
     7201 ggcggacacg gcgcggctga ccaacaccgc gtacaccggt ctgcagctga tcgagacgat
     7261 gaaggcgacc ggtggcgagg acggctactt ccgcaagtgg gccgggcagc acgcgaccac
     7321 cctggaggag cagcagcggc tcggggtgcc gagcgcctgg ctgggcgtgg tcgcgcccac
     7381 gctggcgacg ctgaacagcg cgctgatcct gtggatcggc ggcatgcgcg cggtcgaggg
     7441 ccacatctcg gtcggcctgc tggtcgcctt ccaggcgctg gtggtccgct tcacggcccc
     7501 gctgacccgg ctgaacggcg tcgcgggccg catccaggac ttcgcggccg acgtggcgcg
     7561 gctgaaggac gtggagaact tccgcgccga cccgctctac gaccgtcccg gcggcaccgg
     7621 ttcgacgcgc cggctgcacg ggcacgtcga gctgcagaac gtctccttcg gctacaaccc
     7681 gctcgacaag ccgctgctga gcggtttcga cctgaccgtc ggtcccgggc agcaggtggc
     7741 cctggtgggc ggttcgggca gcggaaagtc gacggtgtcg cggctgatct cgggcctgta
     7801 cgcgccgtgg gacggggtga tccgcgtgga cgggcagcgg ctcgacgaca tcccgcgcgg
     7861 ggcgctggcg tcctccgtgt ccttcgtcga ccaggacgtg ttcctgttcg agggctcggt
     7921 gcgcgacaac gtggcgctgt gggacccctc gatccccgac gacgccgtgg tggaggcgct
     7981 caaggacgcc gcgctgtacg acgtggtgac gcgcaggccg ggcggcatcc acagcccggt
     8041 cgagcaggac ggccgcaact tctccggcgg gcagcgccag cgtctggaga tcgcgcgggc
     8101 gctggtgcgc cggcccagca tcctggtcct ggacgaggtg accagcgccc tggacgcgga
     8161 gaccgagctg gtggtcatgg acaacctgcg ccggcgcggc tgcgcctgcg tggtgatcgc
     8221 gcaccggctg agcaccgtgc gggacagcga cgagatcgtc gtcctggagc acggcacggt
     8281 cgtggagcgc gggcggcacg cggagctggt ggcacacggt ggcgcgtacg ccgcgctggt
     8341 cggggagcgc tgaggtgacg accgtgcggg aagaacacgg cgacctggtc ctcggggcgc
     8401 tgggcgcgct gggcacgcgc gtcgactgcg ccgggtccag ccggctcgac ctggaggggc
     8461 cgcaggtgct gtggctcgtg gtctccggcg cggtggacct gttcgcggtc gacgccgcgg
     8521 agcagggcca ctggcaccac ctggggcgtc tggaggcggg ctcggcgctg ctgggtccgg
     8581 tcgcgggacc gcggcacacg ctggtggcgc gtccgctgcg ggactgcgtc gtgcaccgca
     8641 tcgggctgcg cgagctgtac cagcaggcgc ccacccagac gtggtcgtac gacgcgtacg
     8701 gcaatccgca gtacgtgccg ccggcgacga gcccgctgga gtacgccctg gccctcggcg
     8761 tgggccgggg tctgaccgtc ctcttccagg cgccgatggc cggcgagcgg gccggcgccc
     8821 ccaccgacga cgacgtgttc tggatccagg tgccgccggg cagcgtgcag tacggggcgg
     8881 tgtacggcga ggaggcgtcc gccgacctgc tgatggaccc ggcgctgtgg cagggcgtgg
     8941 tcgaccagca gtaccggctg ctgaccaccc tggaccgctg gatcgagcag ctcgaacgga
     9001 cccatgagac gcggaccgcg gccggcatcg aggccggtga ggcggtgcgc gcgcaggcgg
     9061 accggacgct gctggcctcc ctcggcaggc ggaaggcgcg gcgcaccacg gccgccgacg
     9121 cggacgcgac gtacgcggcc tgccgcctgg tcgcccgcgc ggccgggatc ccgctcgcgg
     9181 agccggcccg cgaccgtacg gagagcgacc ggctcgaccc ggtggagcgg atcgcgctgg
     9241 cctcgcgcgt gcggaccagg tcggtgcggc tggaggagcg ctggtggcgg gacgatgtgg
     9301 ggccgctggt cgggcaccgc accctgtcgg gggcgccggt ggcgttgctg tggcggcgcg
     9361 gcggctatgt ggccgtgcat ccggcgaccg gtcgtgagac tccggtggag aaggcgaacg
     9421 ccggggagtt cgagccgcgg gcggtgatgt tctaccggcc gctgcccgaa cgccggctcg
     9481 gtccgctgcg gctgctgcgg ttctgtctgg cgggcacgcg ccgggacctg gccggtctgc
     9541 tgctcgccgg tctggtgacg gtgggtctcg gcgcgctggt gccggtcgcg acgggaaagg
     9601 tgctcggcga gttcgtgccg aaggcgcaga cggggctgat cgtgcaggtg tgtctcgcgg
     9661 tgatgctgag cggcgtcgtc gcggcggcct tcatgctgct gcagaacctc accatcctgc
     9721 gtctggaggg ccggatcgag gcgacgctgc agcccgccgt gtgggaccgg ctgctgcggc
     9781 tgccgacgag gttcttcacc gaacgctcca ccggcgaact ggccagcgcc gccatgggcg
     9841 tcagcgcgat ccgccgtctc ctcgcggggg tcggcccgac ggtggcccag tcggtgaccg
     9901 tgggggcggt gaacctggcg ctgctgctct ggttcagcgt gccgatggcg ctggcggcga
     9961 tcggcatgct ggtggtcgtc gccggggtgt tcctggcgct cgggctgtgg caggtgcgct
    10021 ggcagcggcg gctggtcaag ctcaccaaca agctgaacaa ccaggcgttc cagaccttgc
    10081 gcgggctgcc caagctgcgg gtggcggcgg ccgagaacta cgcctacgcc gcgtgggcgg
    10141 agcggttcgc gcacagccgt gagctgcagc ggcgggcggg gcggatcaag aacctgaacg
    10201 cggtgctggg cgcggt
//
