LOCUS       NZ_BBOK01000002        10567 bp    DNA     linear   CON 17-AUG-2024
DEFINITION  Streptomyces sp. NBRC 110030, whole genome shotgun sequence.
ACCESSION   NZ_BBOK01000002
VERSION     NZ_BBOK01000002.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. NBRC 110030
  ORGANISM  Streptomyces sp. NBRC 110030
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Katano-Makiyama,Y., Hosoyama,A., Hashimoto,M., Tsuchikane,K.,
            Uohara,A., Kitahashi,Y., Ohji,S., Ichikawa,N., Kimura,A.,
            Yamazoe,A., Komaki,H., Igarashi,Y. and Fujita,N.
  TITLE     Whole genome shotgun sequence of Streptomyces sp. NBRC 110030
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 898909)
  AUTHORS   Hosoyama,A., Kimura,A., Ohji,S., Ichikawa,N., Yamazoe,A. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2014) Contact:Director-General Biological Resource
            Center National Institute of Technology and Evaluation, NITE
            Biological Resource Center; 2-49-10 Nishihara, Shibuya-ku, Tokyo
            151-0066, Japan URL :http://www.bio.nite.go.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 97x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina HiSeq 1000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001417675.1-RS_2024_08_17
            Annotation Date                   :: 08/17/2024 02:44:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,152
            Genes (coding)                    :: 7,019
            CDSs (with protein)               :: 7,019
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 45 of 133
            Pseudo Genes (incomplete)         :: 107 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 23 of 133
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:33:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 343593
            Orig. end                         :: 354160
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBOK01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10567
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10567
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10567
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5567](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5567
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(892..2187)
                     /locus_tag="APK71_RS05930"
     CDS             complement(892..2187)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976094.1"
                     /locus_tag="APK71_RS05930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /protein_id="WP_055467668.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2.1e-30,
                     bitscore: 101.1, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLPPTGSRPRVLHLTQPVDGGVARVVTDLTRAQLAAGFRVAVAC
                     PGGGLADRLRALGADVRDWPATRSPGPSVVAEVRRLVRVVEEVRPDLVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEAVGAAPGLLALGWERWAARWTARTVCVSEAERMRGVRAG
                     LRGPWAVVPNGIDPARFDAAPPADAPADGPEDPDGVRRRHGVAARAPLVVCVGRLCRQK
                     GQDVLLDAWESVLARVPGARLVLVGDGPDRARLAARSPASVLLAGPAPDAAVWYRAADL
                     VVLPSRWEGMALAPLEAMACGRAVVVSDVDGAREGLPPALVPHCLVPPGDPAALAGAVA
                     ALLLDAPLRASLGRRGRAHVRSAHDVRHTAAAVEALYEALLDGVRPTTGAGPGAAARPG
                     PSAVPHPLPGDGPAAAGRSAVVPIECRESTHP"
     gene            2465..2704
                     /gene="chpG"
                     /locus_tag="APK71_RS05935"
     CDS             2465..2704
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="APK71_RS05935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin ChpG"
                     /protein_id="WP_030179666.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVAGTAGVAAADSGAQAAAAHSPGVLSGN
                     VVQVPVHIPVNVCGNTIDIIGLLNPAFGNECEND"
     gene            2990..3739
                     /locus_tag="APK71_RS05940"
     CDS             2990..3739
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976096.1"
                     /locus_tag="APK71_RS05940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_055467669.1"
                     /transl_table=11
                     /translation="MRLPARRIATSVLCAGLLIGLSGPAVMAADGAPARERTHTASRAP
                     LPDAEELQSQVAGLAGLGGVLTPVTDLLDAVLKADNGQLSAADADKLSAAVKDALAKAD
                     AADTDAGDTAATPGTTTPGTSTPAQPPAVTAPESGTDTPVTLPAPVTAPGDDGTAAASD
                     LSASAQAALQKQVDALVKATTSGSADQVSPAVKDVVTSVVNVVAATLVGNGLPAADLAG
                     LPGVPTAPQATAPDATTPEAAAPADPS"
     misc_feature    complement(3807..3824)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.07"
                     /tool="antismash"
     gene            3957..4259
                     /locus_tag="APK71_RS05945"
     CDS             3957..4259
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445910.1"
                     /locus_tag="APK71_RS05945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030400005.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVAAGSAVPAFAYDAADTAALTPTSLNGAVNA
                     LTAEPIDIMPVKHQSDARSNVLDTENKDSVLHTVKGATGKLNSANGLLGGLPLGG"
     gene            4359..4874
                     /locus_tag="APK71_RS05950"
     CDS             4359..4874
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="APK71_RS05950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030400004.1"
                     /transl_table=11
                     /translation="MKPTTRRTLAAVVTGAAAAVGAAVTPAAAAGAIPVPVPLGGAETA
                     LGMELPEVAGELPVPTAGAPEGPRYVEGRLLPERTLPQVPVHGGLPGVDLRQPVPHVLG
                     EDFDHAGLDAPASGLRTLTPGLSLDAPLTAPDPGALGLPSPKLPEAGVLAPALRTAPVA
                     DLGVGPGL"
     gene            5001..5567
                     /locus_tag="APK71_RS05955"
     CDS             5001..5567
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="APK71_RS05955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase cofactor"
                     /protein_id="WP_055467670.1"
                     /sec_met_domain="melC (E-value: 5.6e-49, bitscore: 161.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVTTGTRAHDAATGGAERAREHGAEGAGPAAGRPVSGTRRQLMR
                     GLLAPALAVGLAPLIAASRPPRATEAADGAAGLAHPEPRAAEPVDGAAFDETYRGRRIQ
                     GIRSAGGRAVGAGTWHVTVDGRPLHLMRRADGSWLSMVDHYRSYPTPLAAARGAVDELG
                     PGEHLRATSSAGHDEHSGGRHGVHA"
     gene            5551..6417
                     /locus_tag="APK71_RS05960"
     CDS             5551..6417
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="APK71_RS05960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_031184542.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRSEKRRFVNALLEIKRRGEYDEFVRTHIEYYVS
                     DGDNGLRTAHMAPSFLPWHRRFLLDLEEALRRVDSSVTVPYWDWTKDRSAKSTPWTADL
                     LGGTGRRSDHRVTTGPFAHAEGNWTIKVNVTDTKYLTRDLGRAGNPLDLPSKSDLEWAL
                     DDPKYDTSPYDSTVRKGFRNKLEGWGAGRGSASWHNHNRVHRWVGGAMVGGASVNDPVF
                     WLHHAFVDLQWSRWQARHRGARYLPAEPPGRGSAQRGRIVARHEKLPPWDVTPDELEDV
                     GRIYRYA"
     gene            complement(6580..6855)
                     /locus_tag="APK71_RS05965"
     CDS             complement(6580..6855)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449724.1"
                     /locus_tag="APK71_RS05965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin"
                     /protein_id="WP_030400001.1"
                     /transl_table=11
                     /translation="MSRIAKAAGVALGAGAVALSGAGLAMADAGAQGAAVGSPGVASGN
                     VVQVPVNVPVNVCGNTIDVIGLLNPAFGNACVNDGGHEGHGGGYGG"
     gene            7047..7682
                     /locus_tag="APK71_RS05970"
     CDS             7047..7682
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449721.1"
                     /locus_tag="APK71_RS05970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_053639065.1"
                     /transl_table=11
                     /translation="MSNDRSDSSARASEPSEPSPSPALAALPALFAEESRVRAFAAVAL
                     GAGDAARVARVAGLSAKETAGALRRLRDQGVVASGADGRLVVAHERFRELARAGRPAPA
                     AARDLAAEGDDGPAGIVVRTFVRDGRLVRLPARWTRKKVVLRYIAEQTFAPGVEYPERT
                     VDERLRAWCEDGEVDHVTLRRHLVDLGHLRRSEGVYVRPADSARAGTA"
     gene            complement(7692..8687)
                     /locus_tag="APK71_RS05975"
     CDS             complement(7692..8687)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028521.1"
                     /locus_tag="APK71_RS05975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_055467671.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 3.9e-51, bitscore:
                     168.4, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDYQRVATEVADWSPVADAVDVVVHDTHYPDEDTLAA
                     ALADVDIVVTLRERVPFPGSLIARLPRLRLIVASGMRNSVIDYAAAEAHGVTVCGTASS
                     STPPVELTWALLLGLARGIVPEADGLRAGGPWQQTVGADLHGRRLGLLGLGRIGGPVAQ
                     VGLAFGMRVSAWSQNLTRERTDEVGVELAPSKEELLRTADFVSVHLALGERTRGLLGPA
                     ELALLKPTAYLVNTSRAAIVDQEALLTTLHEGRIAGAGVDVFDTEPLPADHPMRTAPRL
                     LATPHLGYVSRANYATYYGQAVEAIRAHLAGAPVRRLPGPAAPGPASAPA"
     misc_feature    complement(8777..8792)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.39"
                     /tool="antismash"
     tRNA            complement(8849..8924)
                     /anticodon=(pos:complement(352482..352484),aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="APK71_RS05980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Arg"
     gene            complement(8849..8924)
                     /locus_tag="APK71_RS05980"
     misc_feature    9196..9211
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.68"
                     /tool="antismash"
     misc_feature    9198..9212
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.51"
                     /tool="antismash"
     misc_feature    complement(9199..9214)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.93"
                     /tool="antismash"
     misc_feature    complement(9201..9214)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.26"
                     /tool="antismash"
     misc_feature    complement(9223..9237)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.23"
                     /tool="antismash"
     misc_feature    9226..9242
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.29"
                     /tool="antismash"
     misc_feature    complement(9235..9253)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.4"
                     /tool="antismash"
ORIGIN
        1 cgtccagcgc ccgcttgccc gcgccgcccg gcggccgggg cgccggttcc agacgccggc
       61 acgggaaggc ggcgatccgg tccccggccg ggtacgcggg ggcgtgcgcg ggcgtttcgg
      121 cgtccagctc ccacaccgcg cagcccgact ccgccagcga ccgcagcagc gccgcctgcc
      181 ggggccgtac ggaggggtcc acggccagga cgtcccggac gccgttctgc acgaccgccc
      241 gccgcacttc ctcgcccgtg gacagcaccg gcaggcccgt ggtgccgccc gggtcgtcgg
      301 cgacgatccc gacgggccgc accccgcagc gcgggtggcg cagcagcgcc gaggccaccc
      361 gctgcgcggt ggcggcgggg ccgaccacga gggcggcgcg gggacggttc agcagcgccg
      421 cccggtgtct gcggtgcacg acggcccggc cggcgcagcc cgtcaccacg tgcagcagga
      481 agcccgccac cagggcacgc gcggacagcg cgtacgacgg gacgtacgcc gcgacgagcg
      541 ccgccagcac cagccacacc accgcgatcc ggccgcacag tgcgggcagt tcgtcgagtg
      601 cgccggacgg ccgccggtcc gggcgcggcc gggggcgcag cagcagcgag gcggccacca
      661 gcagggccgt gagcacacct tggtgcgggg caccggtgag gaccacggag cccggcaggg
      721 ccgccgcggc gtccgcgacg agcagcggca gcggggagcc cggcctcggc gcgggacgcc
      781 cggagggcag gcggggacct ccggcgccgt cgcgcggcgg caggaccgcc acgggggagg
      841 agccgaggcc gtgcgactcg ccggcggggg aggggacggt gctttccgcg gtcacgggtg
      901 ggtggactcc ctgcactcga tgggcacgac ggccgaccgg cccgcggctg cgggcccgtc
      961 gccggggagg ggatgcggta cggcggacgg accggggcgc gccgcggccc ccggccccgc
     1021 gccggtcgtg ggccgtacgc cgtcgaggag ggcctcgtac agggcctcga ccgccgcggc
     1081 cgtgtgccgt acgtcgtgtg cggaccggac gtgcgcacgc ccccggcggc cgagcgaggc
     1141 gcgcagcggc gcgtcgagca gcagcgcggc gacggcgccg gccagcgccg ccgggtcccc
     1201 gggcggcacc aggcagtgcg gcacgagcgc gggcggcagg ccctcgcggg caccgtcgac
     1261 gtcgctcacc accacggccc ggccgcaggc catcgcctcc agcggggcca gtgccatgcc
     1321 ctcccagcgc gagggcagga cgaccaggtc ggcggcccgg taccagacgg cggcgtcggg
     1381 ggcgggtccc gcgagcagca cggacgccgg ggaccgcgcg gccagccggg cgcggtccgg
     1441 gccgtcgccg acgaggacca gccgggcgcc gggcacccgg gccaggacgg actcccacgc
     1501 gtccagcagg acgtcctgtc ccttctgccg gcacagccgc cccacgcaga ccaccagcgg
     1561 ggcgcgtgcg gcgacgccgt gccgccgccg caccccgtcc gggtcctcgg gaccgtccgc
     1621 gggggcgtcc gcgggcgggg cggcgtcgaa gcgggcgggg tcgatgccgt tcgggaccac
     1681 cgcccagggg ccgcgcagac cggcccgcac accccgcatc cgttccgcct cgctcacgca
     1741 caccgttcgc gccgtccacc gcgccgccca ccgctcccag ccgagcgcga ggagcccagg
     1801 ggcggcgccg accgcctcga acgaccaggc gtgcggctgg aacacggtcg ggatccggcc
     1861 ccgtacggcg agccgtcccg cgagaccggc cttcgcgctg tgcgcgtgca ccaggtcggg
     1921 ccgtacctcc tcgacgacgc ggacgaggcg ccgcacctcc gccaccaccg acggacccgg
     1981 cgagcgcgtc gcgggccagt cgcgcacgtc ggcccccagc gcccgcagcc ggtcggcgag
     2041 acccccgccg ggacaggcga cggcgacgcg gaaaccggcg gcgagctgag cgcgcgtcag
     2101 gtccgtcacc acccgggcga ccccgccgtc caccggttgg gtgaggtgca gaacccgtgg
     2161 ccgggaaccg gtcggtggca ggtgcatgcg cgtttcctcg ctcacggaga tggctcggga
     2221 cgggcgggaa cgcgcccggc cgcggggcgc cggccgcgac gcggcatacg ggtgcgtcga
     2281 atggtgggac tctagctgct atccgtacta atacggtgaa accgggtaac tgtgttggat
     2341 ttgtgaagcc cgcgtttgta cgggatcacc cctttggcgg cacaactcgg ggcccgcgcc
     2401 acgcgttgac actgcgccgg gcagccgccc ggatgtgtgt gacaacccca aggagcactt
     2461 ctccatgtcg cgtatcgcga agggcctggc cctgacctcc gtcgccgccg ccgcggtggc
     2521 gggcaccgcc ggtgtcgccg ccgccgacag cggcgcgcag gccgccgccg cccactcccc
     2581 gggcgttctg tccggcaacg tggtgcaggt cccggtccac atcccggtca acgtctgcgg
     2641 taacaccatc gacatcatcg gtctgctgaa cccggcgttc ggcaacgagt gcgagaacga
     2701 ctgacgtcga ccggcaccac cgcgtaccgg ccgtccacac cgcggttgct ccgcgagcgg
     2761 gcggccggtt cgcgttgccg cgcacacggc gctgtgctgc cccgaatggg gggaccgcgc
     2821 ggcgacacgg cacacgcacc ttgacagggc gtctcagcag gcaggatgcg aggcggcgga
     2881 agagcgcggg acgagcgggc gggccggccg gggcaacgtt cggttgcacg gcgccggggg
     2941 cgcttccacg gtgggcacaa gatccgacgc accaccgaaa ggaaccccca tgcgtctgcc
     3001 cgcacggcga atcgccacct ccgtcctctg cgccggcctg ctgatcggcc tctccggccc
     3061 ggcggtgatg gcggccgacg gcgccccggc ccgggaacgc acccacacgg cgtcccgcgc
     3121 gcccctcccc gacgcggagg aactgcagag ccaggtcgcc ggcctggccg ggctgggcgg
     3181 agtgctcacg ccggtcaccg acctgctcga cgccgtcctc aaggcggaca acggccagct
     3241 ctccgccgcg gacgcggaca agctctccgc cgccgtcaag gacgccctcg ccaaggcgga
     3301 cgccgcggac acggacgccg gggacacggc ggccaccccc ggcaccacca cccccggcac
     3361 cagcaccccc gcccagccgc cggccgtgac cgcgccggag agcgggaccg acaccccggt
     3421 cacgctgccg gcgcccgtca cggcgcccgg cgacgacggc accgcggcgg cctccgacct
     3481 gtccgcgtcc gcgcaggccg cactgcagaa gcaggtcgac gccctggtca aggccaccac
     3541 ctccggctcc gccgaccagg tgagcccggc cgtcaaggac gtcgtgacca gtgtcgtcaa
     3601 cgtggtcgcc gccacgctgg tcggcaacgg gctgcccgcc gcggacctgg ccggcctgcc
     3661 cggcgtaccg accgcgccgc aggccacggc ccccgacgcc accactcctg aggcggccgc
     3721 gccggcggac ccctcctgac gcccagtcac cctcccgggg cgtcccgggc cgtcgcggct
     3781 cccgttcacc ggagcggcgg cggccctttc ggctttccgg ggtacggccg tccggggcct
     3841 cccgtccgga gatctcatat gtcttccgga cggcggagtt tccggttccg gagcaggtcg
     3901 ttggagaggg cgtcagcagt ccaccggtga cccgggtcgc cgaggaaagg aacacgatga
     3961 agtccctcaa ggccgccgcc gtcgtcgccg gttccctggt cgccgcaggc tccgccgtgc
     4021 ccgcgttcgc ctacgacgcc gccgacaccg ccgccctcac gcccaccagc ctcaacggcg
     4081 ccgtgaacgc gctgaccgcg gagcccatcg acatcatgcc ggtcaagcac cagtcggacg
     4141 cgcggagcaa cgtcctggac accgagaaca aggactccgt gctgcacacc gtgaagggcg
     4201 cgaccggcaa gctgaactcc gcgaacggcc tgctgggcgg cctcccgctg ggcggctgag
     4261 ccacccccgg ctccccgccg cgccccatgc gtgccgccct cccgcccccg ccttcccgaa
     4321 gccgtacccc cgccttctcg aaagggaccg tgcctgtcat gaagccgacc acccgacgaa
     4381 ccctggccgc cgtcgtcacc ggtgcggccg ccgccgtggg tgcggcggtg acccccgccg
     4441 ccgcggccgg cgcgattccc gtaccggtgc cgctgggcgg tgcggagacg gctctcggca
     4501 tggaactgcc ggaggtggcc ggtgaactgc ccgtgccgac ggcgggcgcc cccgagggcc
     4561 cgcggtacgt cgagggccgg ctgctgcccg agcggacgct gccccaggtg ccggtgcacg
     4621 gcgggctgcc cggcgtggac ctgcgccagc ccgtgccgca cgtcctcggc gaggacttcg
     4681 accacgccgg cctcgacgcg cccgcctccg gcctgcggac cctgacgccg gggctgtccc
     4741 tggacgcccc gctcaccgcg ccggaccccg gggccctggg cctgccgtcg cccaagctcc
     4801 cggaggccgg cgtgctcgcc ccggcgctgc gcacggctcc ggtggccgac ctgggagtgg
     4861 gcccgggcct gtaggcgcgc ggacccggcg acccccgccc ggcccagcgg ccgtgctccg
     4921 agtggccccg tgtgcggtga ccacggggcc cgccgtgcgg ttagcggggc gaacccacgc
     4981 aaccggacga ggagcggacc atggtcgtca ccacgggcac gagagcgcac gacgcggcaa
     5041 cgggcggcgc ggaacgggcg cgggagcacg gagcggaggg ggcgggcccg gctgcgggcc
     5101 ggccggtgtc cggcacccgg cggcagctca tgcgggggct gctcgccccg gccctcgccg
     5161 tcgggctggc gcccctgatc gccgcctccc ggcctccccg ggccacggag gccgccgacg
     5221 gcgcggcggg actcgcgcac cccgaacctc gggccgccga gcccgtcgac ggcgccgcct
     5281 tcgacgagac ctaccgcggg cggcgcatcc agggcatccg ctccgccggg ggccgggccg
     5341 tgggcgccgg cacctggcac gtcaccgtgg acggccgccc gctgcacctg atgcgccgcg
     5401 ccgacggcag ttggctgagc atggtcgacc actaccggtc ctaccccacc ccgctcgccg
     5461 cggcccgcgg cgccgtcgac gaactcggcc ccggcgagca cctgcgcgcc acctcgtccg
     5521 ccggccacga cgagcactcc ggggggcggc atggcgtaca cgcgtaagga cgtcagcacg
     5581 ctgacccgca gcgagaagcg gcggttcgtc aacgcgctgc tggagatcaa acgacgcggc
     5641 gagtacgacg agttcgtgcg cacccacatc gagtactacg tctccgacgg cgacaacgga
     5701 ctgcgtacgg cccacatggc gccgtccttc ctgccgtggc accggcggtt cctgctggac
     5761 ctggaggagg cgctgcgccg ggtcgactcg tcggtcaccg tgccgtactg ggactggacg
     5821 aaggaccgca gcgccaagtc gacgccctgg accgccgatc tgctgggcgg caccgggcgg
     5881 cggtccgacc accgggtcac caccggtccc ttcgcccacg ccgagggcaa ctggaccatc
     5941 aaggtgaacg tcaccgacac caagtacctc acgcgggacc tgggacgcgc cggcaatccc
     6001 ctcgacctgc cctccaagag cgacctggag tgggcgctgg acgacccgaa gtacgacacc
     6061 tcgccctacg actccacggt ccgcaagggc ttccgcaaca agctggaggg ctggggggcc
     6121 ggccgcggca gcgcctcctg gcacaaccac aaccgggtgc accgctgggt cggcggggcc
     6181 atggtcggcg gcgcctccgt caacgacccg gtgttctggc tgcaccacgc cttcgtcgac
     6241 ctccagtggt cgcgctggca ggcccgtcac cgcggcgccc gctacctgcc cgccgagccg
     6301 cccggccggg gcagcgcgca gcgcgggcgg atcgtcgcac ggcacgagaa gctgccgccg
     6361 tgggacgtga cgccggacga gctggaggac gtgggccgga tctaccggta cgcctgagtg
     6421 gggccgggtc cggggccgtc tccggggccg tcccggtccc tcccgggcct gcgtccgggc
     6481 ttgcgccggg cttgcgccgg gcctgcgtcc gggcccgtct ccggacaagg caggagcccc
     6541 ggccgggaag gccggggctc ctggggtgcc tgggggctct cagccgccgt agccgccgcc
     6601 gtggccctcg tggccgccgt cgttcacgca ggcgttgccg aaggccgggt tcagcagccc
     6661 gatcacgtcg atggtgttgc cgcagacgtt gaccggcacg ttcaccggga cctggacgac
     6721 gttgccggaa gcgacaccgg gcgagccgac ggccgcgccc tgcgcgcccg cgtccgccat
     6781 ggccaggccg gcaccgctga gcgccacggc accggcaccg agggcgactc cggcggcctt
     6841 cgcgatgcga gacatcacgt tctccttctg actcggggag cgcggcggca ggacgcgccg
     6901 ccgcactgcc ggttcaacgc ccccagccgc ggccggtcac ggggggaatg cgcggatcac
     6961 cctttttgag cgcgcggtgc cgggggtgac ggcggcggcc ggaaaaccgt tcccgctcgc
     7021 gccggtgtgt ccactagcct cccggcatgt cgaacgaccg atccgactcg tccgccaggg
     7081 cttccgagcc gtccgaaccg tccccgtcgc ccgcactggc cgcgctgccc gccctcttcg
     7141 ccgaggagag ccgggtgcgg gccttcgccg ccgtggccct gggcgcgggc gatgccgcac
     7201 gcgtggcccg ggtggccggc ctctccgcga aggagacggc cggtgcgctg cgccgactgc
     7261 gggaccaggg cgtggtggcg tccggcgccg acggccggct cgtcgtcgcc cacgagcgtt
     7321 tccgcgagct ggcccgcgcg ggccgtccgg cgccggcggc ggcccgggac ctcgccgccg
     7381 agggggacga cggcccggcc ggcatcgtcg tgcggacctt cgtcagggac ggccggctgg
     7441 tccggctccc ggcacgctgg acgcgcaaga aggtggtgct gcggtacatc gccgagcaga
     7501 ccttcgcacc cggcgtggag taccccgagc gcaccgtcga cgagaggctg cgcgcctggt
     7561 gcgaggacgg ggaggtcgac catgtgacgc tccggcgcca tctggtggac ctcggacacc
     7621 tgcgccggag cgagggcgtc tacgtccgcc ccgcggactc cgcccgggcg ggcaccgcct
     7681 gagcctcgtc cctacgcggg ggcggaagcc gggcccgggg cggcgggccc gggcaggcgc
     7741 cgtacggggg cgcccgcgag gtgggcccgg atcgcctcga cggcctggcc gtagtaggtg
     7801 gcgtagttgg cgcgtgacac gtagcccagg tgcggcgtcg cgagcaggcg cggcgcggtg
     7861 cgcatcgggt ggtccgcggg cagcggctcg gtgtcgaaga cgtccacgcc cgccccggcg
     7921 atgcggccct cgtgcagcgt ggtgagcagt gcctcctggt cgacgatcgc cgcccgcgag
     7981 gtgttgacca ggtaggcggt cggcttgagc agggccagtt cggcggggcc gagcaggccg
     8041 cgggtgcgct cgcccagcgc caggtggacg gagacgaagt cggcggtccg caggagctcc
     8101 tccttcgagg gggccagctc gacgcccacc tcgtcggtgc gctcccgggt gaggttctgg
     8161 ctccaggcgc tcacccgcat gccgaaggcc aggccgacct gggcgaccgg gccgccgatc
     8221 ctgccgaggc ccagcagccc gagccgccgg ccgtgcaggt ccgcgccgac cgtctgctgc
     8281 caggggccgc ccgcgcgcag tccgtcggcc tccgggacga tgccgcgggc gaggcccagc
     8341 agcagggccc aggtcagctc cacgggcggg gtcgaggagg aggccgtacc gcagacggtg
     8401 acgccgtggg cctcggccgc cgcgtagtcg atcacgctgt tgcgcatgcc ggaggcgacg
     8461 atcagtcgca gccggggcag ccgggcgatc aacgagcccg ggaacggcac gcgttcgcgc
     8521 agcgtgacca cgatgtcgac gtcggcgagc gccgcggcga gggtgtcctc gtccgggtag
     8581 tgggtgtcgt gcacgacgac gtccacggcg tcggccaccg gcgaccagtc ggcgacctcg
     8641 gtcgccaccc gctggtagtc gtccagtacg gcacagcgca gtcgcacggc ggcggtccct
     8701 ctcgccgaag gggcagtggc ggccgccgag tccggcgacg cctccggtgg cagggttcgg
     8761 tgccgggacc ggcgtggagg atgatcttct tgcggccccg cgccggctcc ggcagcgtga
     8821 caccggcagc gtggcaccgg cagcactgtg gcgcccccgg caggactcga acctgcggcc
     8881 aagcgcttag aaggcgcctg ctctatccac tgagctacgg gggcctgatg tgtggcctcg
     8941 gtcgtggtgc ccggaactgt ccggtccgtg acgtcgccgg ggacaaggat agggctcccg
     9001 gctccatgtc cctgatgctt cccctccgtg gctctatgtg gaggttcggt gaagcggtcc
     9061 tgataatcgc aggcagttac gaatcgtgca tcgcttttgg cgcctcgcgc atcgggtgtt
     9121 gtccactcgt tatgcctgga ctgtcctcgt gtcccggtag tcccttcagt ccggagtgtc
     9181 cggtcggcgc gcagacatgc tcatcatgct catatgcttc agaattccct caaaattggg
     9241 cattcttcgc atgtggtgac cttggacgta cggcctcagc ttctcgacgc actttccgcc
     9301 ctgcgcgacc gtgtcgcggc cgcacgcttc ccgctgccgc tggccggagc gccacgcgcg
     9361 cgtgccaacc gcgacgaact gctcgcccag ctcgacgact acctcatgcc gcggctgaag
     9421 gaacccgagg cgccgctgct ggccgtggtg ggcgggtcca ccggcgccgg caagtccacc
     9481 ctggtgaact ccctcgtggg gcgccgggtc agcgaggcgg gcgtactgcg gcccacgacc
     9541 cgcacaccgg tcctggtgtg ccacccggag gaccaccact ggttcagcgg catgcgggtg
     9601 ctgcccgacc tcacgcgcgt gtgggtgccc caccggggcc cggacgacga cctgctgctg
     9661 cccggcgaga accccgcgcg cgtcctgcgg gtcgagaccg ccgacagcct gccgcccggc
     9721 atcgccctcc tcgacgcgcc cgacgtcgac tccctcctcg ccgacaaccg cgtcctggcg
     9781 gccgaactga tctgcgccgc cgacatctgg gtgatggtca ccacggccgc ccggtacgcc
     9841 gacgcggtgc cctggcacct gctgcgcacc gccaaggagt acgacgccac cctggtcacc
     9901 gtgctcgacc gggtgcccca ccaggtggtg gccgaggtgt cgcggcagta cggggcgctg
     9961 ctcaccaagg ccgggctcgg cgacgtcccc cgcttcaccg ttcccgaact gcccgagtcg
    10021 gcctggggcg gcgggctgct gccggccagc gccgtggcgc cgctgcgctc ctggctcgtt
    10081 caccgggccc aggaactcga cgcccggcac cacgccatgg cccgtacggc acacggcatc
    10141 ctggactcgc tcaagtcccg gatgcccgag ctggccgggg ccgccgccgc ccagtacgcc
    10201 gccgcgctgc ggctcaccgc cgccgtcgac agcgcctacg acgacgagca cgcgcgcgtg
    10261 cggggccgct tgaaggcggg cgccgtcctc gccggggacg cgctgaagcg ctggcgggcc
    10321 ttccccctcg actgctcccc ggaagagctt ctcgactcgc tggtggagag cctgagcgcc
    10381 ctgctgctgt gcgccgtcac ggccgccgac gaacgcgtcg acgcggcctg gcgcggcgaa
    10441 ccggcggcgg ccgcccccga actcgcggac ggtgccccgg ccccggagag cgccgagcac
    10501 cgcatcggga tggccgtgcg gcggtggcgg cgcgagctgg aggagtacgc cgaggaagag
    10561 gtccgcg
//
