LOCUS       NZ_CFVJ01000010        10300 bp    DNA     linear   CON 04-AUG-2024
DEFINITION  Streptococcus pneumoniae strain 17F/2, whole genome shotgun
            sequence.
ACCESSION   NZ_CFVJ01000010
VERSION     NZ_CFVJ01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001326475.1-RS_2024_08_04
            Annotation Date                   :: 08/04/2024 01:25:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,087
            Genes (coding)                    :: 1,951
            CDSs (with protein)               :: 1,951
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 59 of 136
            Pseudo Genes (incomplete)         :: 74 of 136
            Pseudo Genes (internal stop)      :: 39 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:58:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 37092
            Orig. end                         :: 47392
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CFVJ01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10300
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10300
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10300
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5300](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5300
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            629..898
                     /locus_tag="AP598_RS05295"
                     /old_locus_tag="ERS012477_01044"
     CDS             629..898
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /locus_tag="AP598_RS05295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_01044"
                     /product="YqgQ family protein"
                     /protein_id="WP_000051787.1"
                     /transl_table=11
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIEL
                     MKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            898..1278
                     /locus_tag="AP598_RS05300"
                     /old_locus_tag="ERS012477_01045"
     CDS             898..1278
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /locus_tag="AP598_RS05300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_01045"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_000259256.1"
                     /transl_table=11
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQL
                     IDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLKKQ
                     GFSEIYILSYGLDSWKGKVKTS"
     gene            1367..1594
                     /locus_tag="AP598_RS05305"
     CDS             1367..1594
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /locus_tag="AP598_RS05305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000660078.1"
                     /transl_table=11
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKESQ
                     LDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(1634..2359)
                     /locus_tag="AP598_RS05310"
                     /old_locus_tag="ERS012477_01046"
     CDS             complement(1634..2359)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1154: Pseudouridine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /locus_tag="AP598_RS05310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_01046"
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="WP_001235025.1"
                     /transl_table=11
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGLQ
                     ELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLDRD
                     TTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVCKPA
                     KLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLAESNY
                     RPLNQKELQIIKNYLEMSR"
     gene            2521..4362
                     /gene="typA"
                     /locus_tag="AP598_RS05315"
                     /old_locus_tag="ERS012477_01047"
     CDS             2521..4362
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /GO_process="GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="typA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /locus_tag="AP598_RS05315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_01047"
                     /product="translational GTPase TypA"
                     /protein_id="WP_000164111.1"
                     /transl_table=11
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAERA
                     MDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVVDA
                     YEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQLDF
                     PVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLDYNDF
                     VGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAGDLIAV
                     SGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSRKVEERL
                     QAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEVIVKEIDG
                     VKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPARGLIGYST
                     EFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMSIEERGTIFV
                     NPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPRILTLEESLEF
                     LNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            4382..4636
                     /locus_tag="AP598_RS05320"
                     /old_locus_tag="ERS012477_01048"
     CDS             4382..4636
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /locus_tag="AP598_RS05320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_01048"
                     /product="DUF3165 family protein"
                     /protein_id="WP_000262800.1"
                     /transl_table=11
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVLS
                     FLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     misc_feature    4800..4813
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.31"
                     /tool="antismash"
     misc_feature    complement(4803..4819)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.88"
                     /tool="antismash"
     misc_feature    4897..4912
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     gene            5001..5300
                     /locus_tag="AP598_RS05325"
                     /old_locus_tag="ERS012477_01049"
     CDS             5001..5300
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /locus_tag="AP598_RS05325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_01049"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000850392.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 8.7e-29,
                     bitscore: 92.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKKHRLLALVLISSFTLLGAASAAVQYPDGGVWTYGEGSGGGW
                     AFSNYYHGKKYHYSSIVSRWDGHSDKGEAPAGKTSYAWIWTKWGEQVAFYYDYD"
     gene            5371..7392
                     /locus_tag="AP598_RS05330"
                     /old_locus_tag="ERS012477_01050"
     CDS             5371..7392
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /locus_tag="AP598_RS05330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_01050"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_050315408.1"
                     /transl_table=11
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKDT
                     QEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQES
                     RERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILISLA
                     IFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFLGGGV
                     VLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGRLLLKR
                     LMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGLGDRGKD
                     TENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANTLYVSPSY
                     LDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYGKSGEEASA
                     PLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDSFISLSSWSI
                     NAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETATLILGAIVGI
                     ASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFVFGASLFILSSR
                     DLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            7394..8035
                     /locus_tag="AP598_RS05335"
                     /old_locus_tag="ERS012477_01051"
     CDS             7394..8035
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /locus_tag="AP598_RS05335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_01051"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571283.1"
                     /transl_table=11
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLLN
                     MIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLVGK
                     KLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILADEPT
                     ASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            8139..9491
                     /gene="murD"
                     /locus_tag="AP598_RS05340"
                     /old_locus_tag="ERS012477_01052"
     CDS             8139..9491
                     /EC_number="6.3.2.9"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /gene="murD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /locus_tag="AP598_RS05340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_01052"
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="WP_000863046.1"
                     /transl_table=11
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFED
                     NPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKRIPVLTEVE
                     LAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASDKDT
                     LVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAADFLVL
                     NFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVE
                     NALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQKA
                     LSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEA
                     TDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE"
     misc_feature    complement(9883..9897)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 16.66"
                     /tool="antismash"
ORIGIN
        1 ccttgtcttt ctttgccaga gggttggtct tgctgacaaa gatatgcggt tgcgctgtga
       61 agagatggtg ggctagcagg tgattgtcat ccagcatctt ggttaaaaca tcacggttgt
      121 aactgtttaa gaagaggacc ccgacctcac tgcggaagtt cttgacgtcg tcgataatct
      181 cccaagtccg agtttcacga aggaagagtt cgtatttctc catatcgctt ttcttgagca
      241 aagagacaaa ggcattgacc acaaaggcat agtgttgaga cgaaacgcta aagagttcgc
      301 ggtgggcgac aggattttta tagcgttcct ccagaagctg ggtctgctcg acaacctgac
      361 gggcataaga gagaaactcc atcccatcac gggtcaaggt gattcccttg ggattgcgga
      421 taaagatctc aatgcccatt tcattttcca aatctcgcac tgcattggag agacttggct
      481 gagtgataaa gagttgcttg gctgcctcat tcatggagcc agtttcgacg attttgataa
      541 tataatgtaa ttgttgaatt ctcatgtttt tattgtacca tacttgcctt agaatcgcta
      601 aagaagatag gaaaataagg agcattgtgt gagtctcttc ttgaaaaaaa cacaaaattt
      661 tgctagaatg agtcttatga aaacattcta tgatgtgcag caattcctca aacgatttgg
      721 tattattgtt tacatgggaa aacgcttata tgatattgaa ctgatgaagt tggaactatc
      781 tcggatttac gatgcagggt tgatggataa attagactat ctagaggcag aagcagttct
      841 tcgcagagag cacaaggtag aattggatta tattgagaaa aatggagaaa agaactaatg
      901 gttacttgga ttttgtgggc acttatacta gcaatgttgg cgtggatggg ctttaactat
      961 cttcgtattc gccgtgcggc taaaattgtg gacaatgagg agtttgaagc cttgattcgt
     1021 acgggtcaat tgattgattt gcgcgaccca gcagaattcc acagaaaaca tatccttggt
     1081 gcacgcaata ttccttcaag tcagttgaaa actagtcttg cagcccttcg taaagataaa
     1141 cctgtccttc tctacgaaaa ccaacgtgcg caacgagtta caaatgcagc tctttacttg
     1201 aaaaaacaag gtttttctga gatttatatc ctttcttatg gcttggattc ttggaaaggg
     1261 aaagtgaaga ctagctaacc ttatttccaa attagtcgaa tgataggatg aagctattgt
     1321 tttatagtat aattaatttg tataaaattt aaggagttct tagaacataa aagaaaagaa
     1381 gaaaaatcca ctttttgtgg gtatcttgtc gattattctt ggtttgttat ttccaattgt
     1441 aggcctgatt ttgggtatca taggattggt cttggctatt tcataccaaa aagagtcaca
     1501 attagactat aaaatagaaa agattcttaa tatcttagga attgtgattt ctgtagttaa
     1561 ctggattgta gcaatcgcct taatttttcg gtaaataaaa gaaaaaagct ttagttttaa
     1621 agcttttttt gttttatcga ctcatctcta aatagttttt aatgatttgt aactcttttt
     1681 ggttcaaagg gcggtagtta ctttctgcta aatctgggtt caatgtgaag tccccaaatt
     1741 ggattctttt gaggctagtc accttaacac caaccgagag gaacattttc ttgatttgat
     1801 gaaattttcc ttctgaaatg gtgatagagg cttgactgag ggagggactt gcagatagaa
     1861 tctctagttt tgcgggttta cagacagtac catctaaaaa gacaattccc ttttgaaagg
     1921 cttggatatg gtcaggtgtt agaagtccat taacctcgac ttggtaagtc ttatcgacat
     1981 gatattgggg atggaggagc tgaaagccca agggaccgtt atcggtcaag aggaggagtc
     2041 ccgttgtatc tcggtccagt cggccaacgg catagagctt gtcagactgg atgtcagatg
     2101 gaagcaggtc catgacggtc ggaagttcct tgtctttgtt ggctgtaacg acaccagcag
     2161 gtttatgaag cataagatag gtgtgttcat agccttgaat gattcgatcc tgaaaaagga
     2221 gttcttgtag tcctgtatcg atattttgag ctagggagcg ggctgggcaa ccatcgacta
     2281 gaattttccc tctgaggagt gcttgcttca tggcctttcg gctgattttt tcttgggcta
     2341 ataaattatc taaacgcata ctgtgcctat cttatcataa gtcgcttgct ttgaaaaggc
     2401 ttgacgtgaa aagaaaagcg gagtgaaaac atttacactt gcttgagtta tgttatttat
     2461 ttgaaattat ggtataatcg ttcagttaga aaataaattt tgaatattat agaggaaatc
     2521 atgacaaaat taagagaaga tatccgtaac attgcgatta tcgcccacgt tgaccacggt
     2581 aaaacaaccc tggttgacga attattgaaa caatcagaaa cgcttgatgc acgtactgaa
     2641 ttggcagagc gtgctatgga ctcaaacgat atcgaaaaag agcgtggaat caccatcctt
     2701 gctaaaaata ctgccgttgc ttacaacgga actcgtatca acattatgga cacaccagga
     2761 cacgcggact tcggtggaga agttgagcgt atcatgaaaa tggttgacgg tgttgtcttg
     2821 gtcgtagatg cctatgaagg aaccatgcca caaactcgtt tcgtattgaa aaaagccttg
     2881 gaacaagacc ttgtcccaat cgtggttgtt aacaaaatcg ataagccatc agctcgtcca
     2941 gcagaagtag tggatgaagt cttggaactt ttcatcgagc ttggtgcaga tgacgaccag
     3001 cttgatttcc cagtggttta tgcttcagcg atcaacggaa cttcttcatt gtcagatgat
     3061 ccagctgacc aagaagcgac tatggcacca atctttgaca cgattatcga ccatatccca
     3121 gctccagtag ataactcaga tgagcctttg cagttccaag tgtcactttt ggactacaat
     3181 gacttcgttg gacgtatcgg tatcggtcgt gtcttccgtg gtacagttaa ggttggggac
     3241 caagttaccc tttctaaact tgacggtaca actaaaaact tccgtgttac aaaactcttc
     3301 ggtttctttg gtttggaacg tcgtgaaatc caagaagcca aagcgggtga cttgattgcc
     3361 gtttcaggta tggaagacat ctttgtcggt gaaaccatca ctccgacaga tgcagtagaa
     3421 gctcttccaa tcctacacat cgatgagcca actcttcaaa tgactttctt ggtcaacaac
     3481 tcaccatttg ctggtaaaga aggtaaatgg gtaacttctc gtaaggtgga agaacgcttg
     3541 caggcagaat tgcaaacaga cgtttccctt cgtgttgacc caactgattc accagataaa
     3601 tggactgttt caggacgtgg agaattgcac ttgtcaatcc ttatcgaaac aatgcgtcgt
     3661 gagggctatg aacttcaagt atctcgtcca gaagttatcg taaaagaaat cgacggtgtt
     3721 aaatgtgaac catttgaacg tgtacaaatc gacactccag aagaatacca agggtctgtt
     3781 atccaaagcc tttctgaacg taaaggtgaa atgttggata tgatttcaac tggtaatggt
     3841 caaactcgtt tggtcttcct tgttccagcg cgtggtttga ttggatactc aactgagttc
     3901 ttgtcaatga ctcgtggtta cggtatcatg aaccatacct tcgaccaata cttgccattg
     3961 attccagggg aaattggtgg acgtcaccgt ggtgcccttg tttctatcga tgctggtaag
     4021 gctacaactt actcaatcat gtctatcgaa gaacgtggta cgatctttgt caacccaggt
     4081 actgaggttt atgaaggaat gatcatcggt gaaaactctc gtgaaaatga cttgacagtt
     4141 aacatcacga aggcaaaaca aatgaccaac gttcgttcag ctactaagga ccaaacagct
     4201 gttatcaaga cgcctcgtat cttgacactt gaagagtctc ttgagttctt gaacgacgat
     4261 gagtacatgg aagtaacgcc tgagtctatc cgtttgcgta aacaaatcct taacaaagca
     4321 gagcgtgaga aagctaacaa gaagaaaaaa tcagctgaat aagagctaga aagagagaaa
     4381 gatggtctat ttagtcctag gaattttact gctcctactc tatgtatttg cgacaccaga
     4441 aagcattaaa gggacagtca atatcgtcgc tatggtatgt attttagtgg cactcttgat
     4501 tttattggtt ctatcttttc tgaaaatttt tcaattacca acagaaatat tcctagcaat
     4561 agccatgttg atcctagctt actttagtgt tagagacatc acactcatgc cagtcaaaaa
     4621 aagtaaaaga agataaaaaa gagagttttg gctctctttt ttgctaggaa ttttacaaga
     4681 ttgcaattta tttttgagtt atcaccatct cttttgtttg gataatatga atgggaaaaa
     4741 cttgaataaa attctttaat ggcatctatt ttatatttgg ttagtactgg ttgcatatta
     4801 ttttgaaata aaactactat ataatattat tcatagaggt aatatagtaa aatatctaaa
     4861 aaattcgatt ttttccttga caacaaataa taaaaatgtt attcttatca cataagaaag
     4921 cggtttcaac aaaagagaga ggagggttta ttttgttgca ttgttgctca aggtgtgctt
     4981 tttttgaaag aaaaaataaa atgaaaacga aaaaacatag attacttgct ctagttctta
     5041 tttcaagttt tacattattg ggagctgcat cagctgctgt acaatatcca gatggaggag
     5101 tatggacata tggagaaggt tcaggaggtg gttgggcttt ttcaaattac tatcatggta
     5161 aaaaatatca ttattcatcc atcgtgagca gatgggatgg tcattctgat aaaggagaag
     5221 ctcctgctgg aaaaacctcc tacgcatgga tttggactaa atggggagaa caagtagcat
     5281 tttactatga ctatgactaa tatttagttt tcttagataa ttccccttgt aagaaattac
     5341 agggggaatt ttgtttacaa ggagtctact atgaagaaac tattcatatt attatcaact
     5401 ttttttctca gcttcttcct tgcttggatt attgtcttac gtgcgccaca atatttatat
     5461 gcaagctatg attccgtttc tttacttcgt gtcaaaaaag atactcagga accgacgcgt
     5521 gaggtatttg aacaggaatt ggagaatttt gcaaactcag aacagagttt aatagctaga
     5581 agaattgtag agccgagtaa ggatgggacg actcacttta cttatgcaac ttatggtcag
     5641 ggaactttac caaaagaatt ccaagaagct agccaagaaa gtcgtgaacg tagtgatccg
     5701 ctaaatagtt atctcctttt gtcaggctcc ttgacgaaag aaaagcttgc cgataaatta
     5761 ggagatttgg gttataaagc aagtgctgac cgaaagatac cgccctattt tcttgctttt
     5821 cgaatattac taaatcccct tattttaatt agtttagcaa tatttggctt atctttcttt
     5881 gctttagtga ttatcactcg gattaaggaa atgagagcag caggtataaa actcttttct
     5941 ggtcagactc tcttatccat catggggcat tctttatcta ctgatatcaa atggctcctt
     6001 ctatcagccc tcctttcctt cctaggtggg ggagtcgttc tttttagtca aggtttgttt
     6061 tatcctatct tgttagccac ctatggtttt gggattagtt tctatctgtt gtttttattg
     6121 gcgatttcaa ttttactaat gcttctttat ctaatgagtt tgaattacaa agcattagtt
     6181 cccgttatta aggggagatt actccttaaa cgcctgatga ttttaaccct attgtgtcag
     6241 ttagtagctg tttttacagt aggatacgct gttaagacag gtttgacgtc ttaccaacga
     6301 ttgaaagaac ttgaaatttc aaaacaagca tggcaggata gagcagacta ttatcaaatt
     6361 tcttttggct taggtgatag aggaaaagat acagaaaatc agagcaagtg gtatgccttt
     6421 gccaaggaag caatcgaaga agaacaagct ctttatgtaa aggataatct gctccatttt
     6481 gccaatccac aaggaaaaaa tgaacaggga gagacactgg atacctatag tccagatgct
     6541 aatacgctct atgttagtcc cagttatttg gacaaggaaa aggtcgtggt agatgctgag
     6601 accaaacaga agttagccca tctccaaaaa ggtgagttta tcctcttgct cccagaacat
     6661 ttgcgctctc gagaagcaga acttaagaaa gtttttgaag aaagattgag ttattatgga
     6721 aaatctggtg aggaggcaag tgctcctttg gattatgaga tgaaagcgca cgttagttat
     6781 ctttcaatgg gagaaaagcg gtttgtttat aataacggtg agaatcccgt atctactcag
     6841 tatttgactg atccgatttt agttgtattc acgccgactt ctacaggtga tagttttata
     6901 tccttatcta gttggtctat caatgctgga aaacaactct ttatcaaagg atatgagagt
     6961 gggctagaac tcttgaagaa agctggaatt tatgagcaag tatcctatct taaagaagga
     7021 agaagtgttt atctaactcg ttataatgaa gttcaaactg aaacagcaac tttaatctta
     7081 ggagctattg tggggatagc tagttccttg ttactctttt attctgtcaa tcttctatat
     7141 ttcgagcaat tccgccgaga tatcttgatt aaacgaattt caggtttacg attttttgaa
     7201 acacatgctc agtatatggt tagtcaattt gccagttttg tatttggtgc tagtctcttt
     7261 attttaagca gtcgagactt ggtgattggc ttgctcactt tattagtctt tctagctagt
     7321 gcagttttga cgctttaccg tcaagcgcag aaagaatctc gtgtttctat gacaattatg
     7381 aaaggaaaat aggatgattg aactaaagaa tatatctaaa aaatttggaa gccgtcagct
     7441 attttcagat acgaatcttc attttgaagg tgggaaaatt tatgccttaa tcggtacaag
     7501 tggctgtggt aagacaacac tcttgaatat gattggacga ttggagccat atgacaaagg
     7561 gcaaatcatc tatgatggca cttctcttaa ggacatcaag ccttctgttt tctttagaga
     7621 ttacttagga tacttatttc aagattttgg cttaattgaa agccaaaccg tcaaagagaa
     7681 tctcaatctg ggtttagttg gtaaaaagtt gaaggaaaaa gagaaaatct ctttgatgaa
     7741 acaagctcta aaccgtgtaa acctctctta tttggatttg aagcaaccta tatttgagtt
     7801 atcaggagga gaagcacaac gtgttgcact agcgaagata attttaaagg atccgccttt
     7861 gattcttgca gatgaaccaa ccgcttcctt agaccccaaa aattctgagg aattactttc
     7921 catcctagaa tctttaaaaa atccgaatcg gaccattatt attgcgaccc acaatcctct
     7981 gatttgggag caagtggatc aggtcattcg agttaccgat ttatcacata gatgatatgg
     8041 taagattcag ttagaagaaa gagtcacaaa cacactttgt ggctttttta tttccataaa
     8101 aatggtaaaa tagtaggagt agaaatggag ttcgagacat gaaagtaata gatcaattta
     8161 aaaataagaa agttcttgtt ttaggtttgg ctaagtctgg tgaatctgca gctcgtttgt
     8221 tggacaagct aggtgccatt gtgacagtaa atgatgggaa acctttcgag gacaatccag
     8281 ctgcccaaag tttgctggaa gaagggatca aggtcattac aggtggccat cctttggaac
     8341 tcttggatga agagtttgcc cttatggtga aaaatccagg tatcccctac aacaatccca
     8401 tgattgaaaa ggctttggcc aagagaattc cagtcttgac tgaggtggaa ttggcttatt
     8461 tgatttcaga agcaccgatt attggtatca caggatcgaa cggtaagaca accacaacga
     8521 ctatgattgg ggaagttttg actgctgctg ggcaacatgg tcttttatca gggaatatcg
     8581 gctatcctgc cagtcaggtt gctcaaatag catcagataa ggacacgctt gttatggaac
     8641 tttcttcttt ccaactcatg ggtgttcaag aattccatcc agagattgcg gttattacca
     8701 acctcatgcc aactcatatc gactaccatg ggtcattttc tgaatatgta gcagccaagt
     8761 ggaatatcca gaacaagatg acagcagctg atttccttgt cttgaacttt aatcaagact
     8821 tggcaaaaga cttgacttcc aagacagaag ccactgttgt accattttca acacttgaaa
     8881 aggttgatgg agcttatctg gaagatggtc aactctactt ccgtggtgaa gtagtcatgg
     8941 cagcgaatga aatcggtgtt ccaggtagcc acaatgtgga aaatgccctt gcgactattg
     9001 ctgtagccaa gcttcgtgat gtggacaatc aaaccatcaa ggaaactctt tcagccttcg
     9061 gtggtgtcaa acaccgtctc cagtttgtgg atgacatcaa gggtgttaaa ttctataacg
     9121 atagtaaatc aactaatatc ttggctactc aaaaagcctt atcaggattt gacaacagca
     9181 aggtcgtctt gattgcaggt ggtttggacc gtggcaatga gtttgacgaa ttggtgccag
     9241 acattactgg actcaagaag atggtcatcc tgggtcaatc tgcagaacgt gtcaaacggg
     9301 cagcagacaa ggctggtgtc gcttatgtgg aggcgacaga tattgcagat gcgacccgca
     9361 aggcctatga gcttgcgact caaggagatg tggttcttct tagtcctgcc aatgccagct
     9421 gggatatgta tgctaacttt gaagtacgtg gcgacctctt tatcgacaca gtagcggagt
     9481 taaaagaata aaatatgaaa aaaattgtct ttacaggtgg ggggacggtt ggacacgtta
     9541 ccctcaatct tttgttaatg cccaagttca tcgaagatgg ttgggaagtc cactatatcg
     9601 gggacaagcg tggtatcgaa caccaagaaa tccttaagtc aggtttggat gtcactttcc
     9661 actccattgc gactgggaaa ttgcgtcgct atttctcttg gcaaaatatg ctggacgtct
     9721 tcaaagttgg ctggggaatc gtccaatcgc tctttatcat gttgcgactt cgtccacaga
     9781 cccttttttc aaaggggggc tttgtctcag taccgcctgt tatcgcagcg cgtgtgtcag
     9841 gagtgcctgt ctttattcac gaatctgacc tgtctatggg cttggccaat aaaatcgcct
     9901 ataaatttgc gactaagatg tattcaacct ttgagcaagc ttcgagtttg tctaaggttg
     9961 agcatgtggg agcagtgacc aaggtttcag atcaaaaaaa tccagaacca gatgaattgg
    10021 tggatattca aacccacttt aatcataaat tgccgactgt attgtttgtt ggcggttctg
    10081 caggtgctcg tgtctttaac caattggtga cagaccataa gaaagaacta acagagcgct
    10141 acaatattat caatctaact ggagattcta gtctgaacga gttgagccaa aatctttttc
    10201 gtgttgacta tgtgaccgat ctctatcaac ccttgatgga attggctgat attgttgtga
    10261 cacgaggtgg tgccaatacg atttttgagc tcttggcgat
//
