LOCUS       NZ_CFVJ01000008        10867 bp    DNA     linear   CON 04-AUG-2024
DEFINITION  Streptococcus pneumoniae strain 17F/2, whole genome shotgun
            sequence.
ACCESSION   NZ_CFVJ01000008
VERSION     NZ_CFVJ01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001326475.1-RS_2024_08_04
            Annotation Date                   :: 08/04/2024 01:25:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,087
            Genes (coding)                    :: 1,951
            CDSs (with protein)               :: 1,951
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 59 of 136
            Pseudo Genes (incomplete)         :: 74 of 136
            Pseudo Genes (internal stop)      :: 39 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:58:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 51999
            Orig. end                         :: 62866
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CFVJ01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10867
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10867
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10867
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5867](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5867
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(657..1532)
                     /locus_tag="AP598_RS04470"
                     /old_locus_tag="ERS012477_00875"
     CDS             complement(657..1532)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /locus_tag="AP598_RS04470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00875"
                     /product="substrate-binding domain-containing protein"
                     /protein_id="WP_000669493.1"
                     /transl_table=11
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISRENG
                     SGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSLTK
                     SVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVVTDN
                     KFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNGSSAG
                     ITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAELADVFS
                     GKLTTWDKIK"
     gene            complement(1699..3030)
                     /gene="pnpS"
                     /locus_tag="AP598_RS04475"
                     /old_locus_tag="ERS012477_00876"
     CDS             complement(1699..3030)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene="pnpS"
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /locus_tag="AP598_RS04475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00876"
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="WP_000833277.1"
                     /transl_table=11
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGGY
                     WLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKDLA
                     ETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLVHQA
                     FDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLNLAFV
                     SNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLLTLSKA
                     QQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQILLNLL
                     SNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNKGRSRQTG
                     GTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(3031..3738)
                     /locus_tag="AP598_RS04480"
                     /old_locus_tag="ERS012477_00877"
     CDS             complement(3031..3738)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /locus_tag="AP598_RS04480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00877"
                     /product="response regulator transcription factor"
                     /protein_id="WP_000166475.1"
                     /transl_table=11
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAETE
                     PFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLTKP
                     FSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLSLTP
                     KEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKAPQFI
                     RTIRGYGYKFKEL"
     gene            complement(3822..4298)
                     /locus_tag="AP598_RS04485"
                     /old_locus_tag="ERS012477_00878"
     CDS             complement(3822..4298)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /locus_tag="AP598_RS04485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00878"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_050301148.1"
                     /transl_table=11
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSIY
                     FLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEVVTLGMDISKGQQLHYCVP
                     KGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERADLLATYPEHKEMIEHLTRY"
     gene            <4305..4851
                     /locus_tag="AP598_RS04490"
                     /old_locus_tag="ERS012477_00879"
                     /pseudo=""
     CDS             <4305..4851
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /locus_tag="AP598_RS04490"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="ERS012477_00879"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="QWVEVTLVQSGFTISRTPLSSEDTVMIDATEVKINRPKRRISELL
                     W"
     gene            5001..5255
                     /gene="blpM"
                     /locus_tag="AP598_RS04495"
                     /old_locus_tag="ERS012477_00880"
     CDS             5001..5255
                     /codon_start=1
                     /gene="blpM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /locus_tag="AP598_RS04495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00880"
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="WP_000379879.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.6e-20,
                     bitscore: 65.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGLG
                     TAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            5271..5474
                     /gene="blpN"
                     /locus_tag="AP598_RS04500"
                     /old_locus_tag="ERS012477_00881"
     CDS             5271..5474
                     /codon_start=1
                     /gene="blpN"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /locus_tag="AP598_RS04500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00881"
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="WP_001099492.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.5e-13,
                     bitscore: 43.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGPW
                     GAAIGGIGGAVVCGYLAYTATS"
     gene            5718..5867
                     /gene="blpO"
                     /locus_tag="AP598_RS04505"
                     /old_locus_tag="ERS012477_00882"
     CDS             5718..5867
                     /codon_start=1
                     /gene="blpO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /locus_tag="AP598_RS04505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00882"
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="WP_001818346.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.2e-11,
                     bitscore: 36.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDGC
                     ATTV"
     gene            5971..6090
                     /locus_tag="AP598_RS04510"
     CDS             5971..6090
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /locus_tag="AP598_RS04510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346296.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     misc_feature    complement(6226..6239)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.08"
                     /tool="antismash"
     misc_feature    6309..6322
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.42"
                     /tool="antismash"
     gene            <6600..6793
                     /locus_tag="AP598_RS12695"
                     /pseudo=""
     CDS             <6600..6793
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /locus_tag="AP598_RS12695"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="NDDLVSPRTNQLMFIGLADFMFVICLYRGISETEFYQQLIACIGV
                     FSACLSRFCSCGA"
     gene            6876..7259
                     /locus_tag="AP598_RS04520"
                     /old_locus_tag="ERS012477_00883"
     CDS             6876..7259
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /locus_tag="AP598_RS04520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00883"
                     /product="hypothetical protein"
                     /protein_id="WP_000877381.1"
                     /transl_table=11
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGVF
                     LLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFI
                     VFNALMYLLSSSQVSLKKNKLSS"
     gene            7311..8000
                     /locus_tag="AP598_RS04525"
                     /old_locus_tag="ERS012477_00884"
     CDS             7311..8000
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /locus_tag="AP598_RS04525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00884"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000760527.1"
                     /sec_met_domain="Abi (E-value: 1.1e-11, bitscore: 37.6,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLFW
                     IQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKHVQ
                     STAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDLLLP
                     SILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFPFLLT
                     FLHRVLG"
     gene            8042..8290
                     /gene="blpZ"
                     /locus_tag="AP598_RS04530"
                     /old_locus_tag="ERS012477_00885"
     CDS             8042..8290
                     /codon_start=1
                     /gene="blpZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /locus_tag="AP598_RS04530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00885"
                     /product="immunity protein BlpZ"
                     /protein_id="WP_000276499.1"
                     /transl_table=11
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFNF
                     LNSMLALMAIFIGAGYVVGFWLLKWFVLERLDLKNDV"
     gene            8320..8931
                     /locus_tag="AP598_RS04535"
                     /old_locus_tag="ERS012477_00886"
     CDS             8320..8931
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /locus_tag="AP598_RS04535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00886"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000394045.1"
                     /sec_met_domain="Abi (E-value: 3.2e-21, bitscore: 68.2,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSLL
                     VTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHLAE
                     VSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSNVPS
                     FIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            9092..9886
                     /gene="ccrZ"
                     /locus_tag="AP598_RS04540"
                     /old_locus_tag="ERS012477_00887"
     CDS             9092..9886
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /GO_function="GO:0004747 - ribokinase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_function="GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /GO_process="GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ccrZ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /locus_tag="AP598_RS04540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00887"
                     /product="cell cycle regulator CcrZ"
                     /protein_id="WP_000363002.1"
                     /sec_met_domain="APH (E-value: 1.8e-19, bitscore: 63.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGLA
                     REQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQLS
                     RLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDVRHS
                     NWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTVLSKL
                     YWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            9883..10518
                     /gene="trmB"
                     /locus_tag="AP598_RS04545"
                     /old_locus_tag="ERS012477_00888"
     CDS             9883..10518
                     /EC_number="2.1.1.33"
                     /GO_function="GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="trmB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /locus_tag="AP598_RS04545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00888"
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="WP_001266078.1"
                     /transl_table=11
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVEV
                     GSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTDYF
                     EDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFEYSL
                     VSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
ORIGIN
        1 gccaagctac cagaataata cgttttggga actgttcgga tagcagattc tgacaaactg
       61 ataatggttg gcaaaatcat tattcctaat agtaacgaag cggttaggac actcatgcca
      121 tttcctaaaa agcttctaat ccaaggcacc aataattgta ggccgaaaaa accataaaca
      181 atagatggaa tggccgccat caagttgata gctgatttta agaagccata gacgggcttt
      241 ggacaataat aaaccataaa caccgatgtc aagatgcctg ttggcacccc aatcacaatc
      301 gctcctaagg taattaataa ggaaccaacg atcattggta aaataccata gcttgccgga
      361 atgttcgttg gcgaccaatc actgcctaat aaaaaacggg caaagccgta gttagccatg
      421 aaaggtaagc cattactaaa aataaagaaa cagattagca aaatagctac aacagctact
      481 gttgcactca tgaaaaaaat tgccctaaaa actgcttctt tgaaggcttg ttttgtcaca
      541 tcttgtcctt tctagtgaag aaagtaaggg agatacgaca cctccctact tgccttcttt
      601 atcttattgt acgatgaaac gtctgcatct ctttagagat ttatggagca aacattttat
      661 ttaatcttgt cccaggtggt taatttgcca ctaaaaacgt ctgcaagttc agccatactg
      721 acttggcttg ccttattgtc attattgacc acaacagcaa taccgtctaa agcaatagca
      781 tcatgggtga gactcttacc ttcttcagga gttaattccc tagaaaccat accaatatca
      841 gcggttttct ccttaacagc ggtaatacct gctgaagacc cattagaggt aatatcaatc
      901 gtaacttctg gattttcttt tttataagct tctgctaatt tttccattaa agaagatact
      961 gaagtggaac ctacaacaga caacttgcct gataagtgtt ggcttgtata ttctgtggtt
     1021 tcagttttag cttcaataaa tttattatct gtgaccactt gttgaccttg tttggagtgg
     1081 ataaagctga taaaatcttg acctagcttg gaaagattag aagaccaaac aatgttgaag
     1141 ggacgttgaa gagggtattc accatctaaa actgtgtctc gactagcctt gacaccatca
     1201 atctctaaag ccttgacaga tttcgttaaa gatcccaagg agatgtagcc gatagcatta
     1261 gcattccctt gaactgctga gagaacacct tctgtactat tttgaatcac agctgttttg
     1321 gcagtgttgt caattttttt atcaccgtct tttttgagaa tccctgtgat ttctgtgaag
     1381 gcaccccgtg ttccagagcc attttctcgt gaaatcacct caatcgttcc tgaagctgac
     1441 tgtttggaag cagctgactg attgccacag gcaacaagcc caaatcctga taagccaatg
     1501 gctgcaagag taagcatttt tttgaatttc ataataatca cctttatctc tatgtatttt
     1561 tcttgtgtag gcttactaca tttatagtct aacaagtctt tgtaaaggtt tatccctgat
     1621 tcatgtaaag attgtgtaaa gaatcaaaaa aagccacttt tgaaaaatgg ctgctcctaa
     1681 aaatagcttt aaaaattatt agtcctgtgc gaaagattgg ttaggaagaa aaatcgtgaa
     1741 gcaactgcct ctgccaagct gactcgtcac cgtgacttgg ccacctaata attgactgag
     1801 ttctttgaca atggcaaggc caagaccagt gccaccagtt tgtctgcttc gacctttatt
     1861 aactcgataa aaacgttcaa aaatacgatc ctgctctaat tgactaatac caatccctgt
     1921 atctgataca gaaatcttaa tgccttcgtt caccttttgg gtcttgacct caatttttcc
     1981 cccttgttca gtgtaacgga tggcattgga taaaagattg agtaagattt gggaaagtaa
     2041 ttgactatct gatacgaggg tgacatcatc tggcacctgc acctttagct gtaaatcctt
     2101 cttcttgagc tgaggttgca agctttgagt caaatcctgt acaaattcag ccaaagaaag
     2161 ggtcgtccat tgtataggca tttgttgagc cttagataag gtaagaagat gctcaacaat
     2221 atgctcaaga cgcaaacttt ctttgtaaat aatgtctaga aagtcatcct tgagcgcttc
     2281 ttcttcagct gacatcccct taatggtttc agcaaagccc ttaattgaag taactggtgt
     2341 cctcaattca tgggaggcat ttgagacaaa ggctaaattt aacttttcat aagttctaat
     2401 cgttgttaaa tcatatagca agacgagcac agcttccaca gattgggtgg ggctaaaaac
     2461 gggaactgct gtcacttcta aaatcaagtc accctcatga aacccactta cttcttgttt
     2521 taaccttgtt ttttgatcaa aggcttggtg aactaaattc cgaatatcca tccgtttgag
     2581 gtcatcaagt gaacttatgt cgccgtccac atcgggaaaa taatgaggca gagagcgact
     2641 ggataataac atctgacctt gagcggaaac taaaaacgtc cccatggtta ggtgcgacag
     2701 aagaacctcc attgtttcgg ctaggtcctt gtattgctga tcctgttggg agactttggt
     2761 ttttaggcca gacacatact gagccaaaga ctttaagtct tcttgccctt tttctaaaaa
     2821 gtattcacta ctggtcaaga gaggttggtg caaggtctca aaagcaactt cccatttcca
     2881 aaggcaaaag agccagtagc caccttgtcc caaagaaagg gctagaagaa agagaccgat
     2941 gcctttactg atccaagtta atgccatccc tgcaatcaga atgaggctaa cacttagatt
     3001 gactagccaa aattgaaggt agcgtttcat ctataactcc ttgaacttat aaccataacc
     3061 ccgaatggtt cgaataaatt gaggggcttt aggattgtct tcaatttttt ccctcaactt
     3121 accaatatga acgtccacca aacgtgtttc ctgcccaaag tcataccccc agatacgttc
     3181 caaaagacgc tctctagtca gtgtcatgtt gggatgtttc ataagataga gcaagagttc
     3241 aaattctttt ggggtcaaac tcagtaactt attcgccttg tagacttcat gacgctcagg
     3301 gtatactttc aaggtcccaa atagccaaga atcgtcagcg atattatctg aatcatctcc
     3361 ttcttgttct cctttagttc gcctgaggac agccttgaca cgcgccagca attctctagg
     3421 gctaaaaggc ttggtcaggt agtcatcagc ccctaattcc aaggccaaaa ccttatcaaa
     3481 ttcatcactt ttcgcagaaa ccatcataat tggagttttg acgcctttgg ctctcagccg
     3541 cttacaaact tccatgccat ctaattgtgg taacatgata tcaagcaaga taaaatcaaa
     3601 gggttctgtt tctgccaaag ctaaggcctt ccgtccattt gttaccaatt gagtagaaaa
     3661 gccttcctta cttaaatggt agtcaagcaa tttcagaatg tgttcttcat catccactaa
     3721 taagacttgt tttgtcatct attatctcct attggtaaca ttataacaca attatcagaa
     3781 atcctaacat tgctaaatta gattaaattt gcttatcaag actagtatct ggtcaaatgc
     3841 tcaatcatct ccttgtgctc tggataggtc gccagtagat ctgccctttc aaataattca
     3901 aaatcctcaa attcaaaacc aggagcaaca agacaagaaa ccagagcata atccttatca
     3961 actgttgatc cccaaatagt gcccttagga acacagtagt gaagttgttg ccctttggat
     4021 atgtccatgc ctaaagtgac tacttcgtag tgaccatctg ctgtaatcat gtgaacagta
     4081 agtggggatc ctgcatgaaa ataccagatt tcatctgctg tcaatcggtg aaaatgtgaa
     4141 ggattcgttt cttctaataa gaaataaata ctggtataaa gcgcccttcc cttaccagca
     4201 aggtttatag tgtctgaagc tttttttgtt tgtctaaaat agccaccttc aacatgggga
     4261 gctaactcta gagttcttat caagtcttct ttatccatcg gggccaatgg gttgaagtaa
     4321 ctcttgttca aagtggtttt acgatttcaa gaactcctct cagttctgag gacacggtaa
     4381 tgattgatgc gacggaagta aaaatcaatc gccctaaaag aagaattagc gaattattat
     4441 ggtaaaaaga aatgccacgc tatgaaggct caagcgattg tcacaagtca agggagaatt
     4501 gtttctttgg atatcactgt gaactattgt catgatatga agttgttcaa aatgagtcgc
     4561 agaaatatcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg gctcatgaag
     4621 atatatcctc aagcacaaac ttcacgtaaa tccagcaaac tcaaaccgct aacagttgaa
     4681 gataaagcct ataaccatgc gctatctaag gagagaagca aggttgagaa catctttgcc
     4741 aaagtaaaaa cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgcttcgga
     4801 ttacgaatga atttgattgc tggtattatc aatcatgaac taggattcta gttttgcagg
     4861 aaatctaatg taatttttgc aattcaagac gtttcgatga ctattcaaga tctggatgaa
     4921 aaattttaaa aaacgatgat atactaaact tgtcaaagtt acaacaagac aaaaattaaa
     4981 aataaaaaag agtatttgtc atggatacaa aaattatgga acaatttcat gaaatggata
     5041 taactatgtt atctagtatt gaaggaggca agaataattg gcaaactaat gtcttagaag
     5101 gtggtggtgc tgcttttggt ggctgggggc tagggacagc tatttgtgct gcgagtggtg
     5161 ttggagcacc atttatggga gcatgtggat acataggagc taaatttggt gtggatcttt
     5221 gggcaggagt aacaggggca acgggtggat tttaataaaa ggagacttgt atgaatactt
     5281 attgtaatat aaatgaaaca atgctgagtg aggtttatgg aggtaattcc ggaggagcag
     5341 ctgtagttgc tgctttaggt tgtgcagcgg gtggagtgaa atatgggaga cttctaggac
     5401 catggggcgc tgcaatagga ggaattggag gagcagtagt ttgtggatat ttagcctata
     5461 ccgctacatc ataaaaatga aagctaaatt ttatgaaggc tatattcttt attattctct
     5521 tcgcttttca aacctatctt atttatctat ccataagtat ctccgataaa aaacaaaaga
     5581 ctattgaatt aacatcattg aattgttttg tcatattatt tctaatctat gataaattaa
     5641 tttttctctt tattgcctat gtttttttga taatttttat attaaacttg ttccgtaact
     5701 gaaaagtgat atactatatg gatacaaaaa tgatgtcaca atttgcagtt atggataatg
     5761 aaatgcttgc ttgcgttgaa ggtggagata ttgattgggg aagaaaaatt agttgtgcag
     5821 caggggttgc atatggcgca attgatgggt gtgcaacaac ggtttgacat ttctattggg
     5881 accatttgct ataggaatag gtgtaactgg tgctgcaggt ggagctatac ttggaggtgt
     5941 agcctatgca gcaatatgtt ggtggtaatt atggatttta aaagttttat tattggttta
     6001 gtagttggta tatttggtcc ttatatggat gacttaatta gaaaaatatt ttcaaaatct
     6061 tccaaaaagg atacggatag tactctctaa aaaataaatt tctcccctgt atttgctagc
     6121 ttaatttaca ggggagtttc tttaatattg tttcgcctgt caaaatgata tgtgatagca
     6181 ggatgaaaag tctacaaaca agttggtatc aatacaatat aaagaggaaa gtatgttttt
     6241 aatgcttact ttttaaattt tttaacaatt caagatgttt cgatgacaat tcaagatttg
     6301 gatgaaaaat tttaaaaaac aatgatatac taaacttgtc aaagttgcaa cgggtgctgc
     6361 tacattatgt taaaaatcgc tcttgattga taagttggat ttgttaggaa aggagtaaat
     6421 aattatggat aagaaaaaga tagtttcgac tataatatgt atagtatttc ttgtagtgtc
     6481 agtagataat ttttttagag acttaacccc gttactgttc attttaaata ttattggcct
     6541 gtcttgcttt tcagtgctaa catacattaa cataaaagag atactcttaa atataagtaa
     6601 atgatgattt ggttagtcct agaaccaatc aacttatgtt tatcggcttg gcagatttta
     6661 tgtttgtcat ttgtttgtat agaggtattt cagaaacaga attctatcaa caactaatcg
     6721 cttgtatagg tgtattctct gcttgtttat cacgcttctg ctcatgtggg gcttgaagta
     6781 ttataaaaag tagctttcta tccttttctg acaggatatt tgagtagaag gctctattaa
     6841 attgttagtg aaataaatga atggaggtat ttaatatgaa atacagatta ttttttgtta
     6901 ttttcttgag tagtgtgttg gatattcttt tagggacatt tttacaaatc tctatcgtat
     6961 ctattgggtg gcttgttctt tacagtggtt tgtttgaagc aggagttttc cttcttgcta
     7021 ataaaggggt ggcggtaaaa atcaaggaag tagatattcg aaatcgcttt aaatttattt
     7081 ttggaaaaac cttatggttt caaattcttt tgctcatctt tttgataatc aaactttatc
     7141 ttggtttgga tgcgaggttg attttattct atggacatat tttcattgtc tttaatgcct
     7201 taatgtatct attatctagt agtcaggtta gccttaaaaa aaacaaactg tcttcttaat
     7261 cttacagcag tagagcctga caaggtgagg tcatagtagg gaggatttct atgaaaaagt
     7321 atcaacttct attcaaaata agtgcagtct tctcttactt atttttcgta tttggtcttt
     7381 ctcagctgac gcttatcgtc caaaactatt ggcaattttc ttctcagata ggcaatttat
     7441 tctggattca aaatatcttg agtttacttt ttattggagt catgattgtg gttcttgtta
     7501 agacaggcca tggttatctc ttccgcattc caagaaaaaa atggctttgg tattcgattt
     7561 tgacagtatt agtggtagtg ttccagatct cttttaacgt tcagacagct aaacatgttc
     7621 agtcaactgc ggaaggttgg gctgtattga ttggttatag tgggactaac tttgcagagc
     7681 taggtattta tatagccctg ttctttctgg ttccactgat ggaagaattg atttatagag
     7741 gattactgca acatgctttc tttaagcatt cgcgatttgg tcttgatttg cttcttcctt
     7801 ctattttatt tgctctccct catttttcaa gcctgcctag tctgttagat atcttcgtct
     7861 ttgcaacagt tggaatcatc tttgctggtt tgacccgcta taccaagagc atttatccat
     7921 cctatgcggt gcatgtgatc aataatattg tagcgacctt cccgtttttg ctcacttttc
     7981 tacatagggt cttggggtaa aaaaacaaaa ggcttgcttt tcagccatag aggaggtcat
     8041 catgtataaa cacttatttt tcctagattc caaaacttta gatcggttga caccctatat
     8101 tctagtcttg gcttctgaca ccattgcttt taatgttttt gtgctaacct ttgtatctgc
     8161 ggtggttttt aatttcctaa attccatgct agctttaatg gctatattca taggggctgg
     8221 ctatgtggtc ggattttggt tgctaaaatg gtttgttttg gaaaggctag atctaaagaa
     8281 tgacgtgtag gaagtctgtt tgttgaggag gataatttta tggagttttt tgataaattt
     8341 catgccttgt gttttggatt tttagtacta ataattgtca ttacagttcc ttatacgatt
     8401 aaccatgggg gtttttttca aaatgaatct gcattgattc ttgtaagtct tcttgtaacc
     8461 tcgctgagtg ttgcttatgc tagaaagttt gaaatgattt cttttgggat gttaagcaag
     8521 aaacaacttt tgcttttcat tgcaatcttt cttctaagtg tacttgagac gctggtttat
     8581 attcatttct tcgctgtttc ttctggctca ggggtccaac acttggcgga agtcagcaga
     8641 ggaatttccc tgtctttgat tttaactacc tcagtttttg gccccatcca ggaggaactc
     8701 attttcagag gacttcttca aggtgcggtt tttgacaatt cttggttagg gcttgtgcta
     8761 acttcctctc tcttttcttt catgcatgga ccttctaatg tcccttcgtt tattttttat
     8821 ctacttgggg gtttgttgct gggctttgct tataaaaaga gtcaaaacct atgggtttct
     8881 actctagtcc atatgcttta caacagttgg ccactcttat attatttata aaaattatga
     8941 aaaggtaagc agaagacggt gcttaccttt ttcaagctat aatgataccc aagccaatcc
     9001 agaggctttt ctttgagaat cgggttagag tggttgacga ataggccaaa aactagtaga
     9061 atagtaagga aactttatac ggaggaaaga aatggatttg ggtgataatg agctaacact
     9121 gactcccata cctgggaaaa gtggcaaggc ttatatgggt agctatcctg atgggaagcg
     9181 catctttgta aaaatgaaca cctctccaat cctacctggt ctagctagag aacaaattgc
     9241 tccacaatta ttatggagtc gtcgtttggc agatgggcgt gatatgtgtg ctcaagaatg
     9301 gttgacaggc aagatattga ccccctatga tatgaatcgt aagcaaatcg tcaatatttt
     9361 aacccgtctt catcgctcac gtccgttgat gacacaattg agtcgtttgg gctatgccat
     9421 ggaaacacct gtagatttac tacagtcttg gcaggaaacg gctccagatg ctttgcgtaa
     9481 aaatcatttt atcagtgaag tgatggctga tttacgtcag actattccag gatttagaga
     9541 ggaccatgcg accattgtcc atggagatgt acgacatagt aattggattg agacagatag
     9601 tggcttgatt tatttagtag attgggattc ggttcgcttg accgatcgca tgtttgatgt
     9661 ggcccatatg ctctgccatt atatttcaga acatcagtgg aaggaatggt tgacctacta
     9721 cggttacaag tacaatcaaa cggtattaag taaattgtat tggtatggtc aattgtctta
     9781 tttgagtcag atttccaagt attatatgaa ccaagattta gaaaatgtca atcgggagat
     9841 tcatggtttg cgtcatttcc gagacaagta tggaaagaga agatgagagt tagaaatcgt
     9901 aaaggggcaa cagaattact agaggcaaat ccccagtatg tggtcctcaa tcccttggaa
     9961 gccaaggcaa aatggcggga cttgtttggc aatgataatc ccattcatgt ggaagttgga
    10021 agtggaaagg gtgcctttgt ttcaggtatg gccaagcaaa accctgacat caactatatc
    10081 gggattgata ttcaaaagtc tgttttgagc tacgctttgg acaaggtgct tgaagctgga
    10141 gtgtctaaca tcaagctctt gtgggtagat ggttctgact tgactgacta ctttgaagac
    10201 ggtgagattg atcgcttgta tctgaacttt tcagatccat ggccgaaaaa acgccatgaa
    10261 aagcgtcgtt tgacctacaa gacctttttg gataccttca aacgtatctt gcctgaaaat
    10321 ggagaaattc atttcaagac ggataaccgt ggcttgtttg agtacagttt agtgagcttt
    10381 tctcaatatg gcatgaaact caatggtgtc tggctagatt tgcatgccag tgattttgaa
    10441 ggcaatgtca tgacagaata cgagcaaaaa ttctcaaaca aggggcaagt tatctaccga
    10501 gttgaggcag aattttaaga gatagcctaa aattaggctg tacaagtgct tttgctttac
    10561 ataagttggc aaacgtgcta tactgatagt aagaatatga aaagtgaggc ggggaaatat
    10621 cttcgcctct tgcttatgag gaggtggacg caatcgcaac aatcgtagaa ttagtcagag
    10681 aagttgtaga acctgtcata gaagctcctt ttgaactcgt ggatatcgag tatggaaaga
    10741 ttggcagtga catgattctc agtatttttg tagataaacc cgaaggaatt accttgaacg
    10801 acacggcaga cttgacagaa atgatcagtc ctgtcctaga caccatcaag ccagatccct
    10861 tcccaga
//
