LOCUS       NZ_CFVJ01000005        10297 bp    DNA     linear   CON 04-AUG-2024
DEFINITION  Streptococcus pneumoniae strain 17F/2, whole genome shotgun
            sequence.
ACCESSION   NZ_CFVJ01000005
VERSION     NZ_CFVJ01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001326475.1-RS_2024_08_04
            Annotation Date                   :: 08/04/2024 01:25:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,087
            Genes (coding)                    :: 1,951
            CDSs (with protein)               :: 1,951
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 59 of 136
            Pseudo Genes (incomplete)         :: 74 of 136
            Pseudo Genes (internal stop)      :: 39 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:58:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 29815
            Orig. end                         :: 40112
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CFVJ01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1009..1143
                     /gene="rpmH"
                     /locus_tag="AP598_RS03050"
                     /old_locus_tag="ERS012477_00593"
     CDS             1009..1143
                     /GO_component="GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /locus_tag="AP598_RS03050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00593"
                     /product="50S ribosomal protein L34"
                     /protein_id="WP_000831905.1"
                     /transl_table=11
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA"
     gene            <1358..2071
                     /locus_tag="AP598_RS03055"
                     /old_locus_tag="ERS012477_00594"
                     /pseudo=""
     CDS             <1358..2071
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_367670414.1"
                     /locus_tag="AP598_RS03055"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00594"
                     /product="DUF1542 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="VAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEYKLE
                     NTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEK
                     AAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAM
                     VTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVA
                     TSGFALLGRKTRRRK"
     gene            2377..3150
                     /locus_tag="AP598_RS03060"
                     /old_locus_tag="ERS012477_00595"
     CDS             2377..3150
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /locus_tag="AP598_RS03060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00595"
                     /product="TatD family hydrolase"
                     /protein_id="WP_000575607.1"
                     /transl_table=11
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIEH
                     ALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTAPK
                     EVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLEWAE
                     KFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENKTAYT
                     RYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            3150..3710
                     /gene="rnmV"
                     /locus_tag="AP598_RS03065"
                     /old_locus_tag="ERS012477_00596"
     CDS             3150..3710
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /locus_tag="AP598_RS03065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00596"
                     /product="ribonuclease M5"
                     /protein_id="WP_000664448.1"
                     /transl_table=11
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERIQ
                     RLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEHAS
                     YEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSNGKQ
                     LLKRLELFGVTLAEVEEAMKSYE"
     gene            3782..4681
                     /locus_tag="AP598_RS03070"
                     /old_locus_tag="ERS012477_00597"
     CDS             3782..4681
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /locus_tag="AP598_RS03070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00597"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000904834.1"
                     /transl_table=11
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPSL
                     PKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEIYK
                     TYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLLIRL
                     FFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVGVTDC
                     FNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAKLIGDG
                     FLVKKLTEEWQEDVKKYL"
     misc_feature    4752..4761
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     misc_feature    4838..4851
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.05"
                     /tool="antismash"
     gene            5001..5297
                     /locus_tag="AP598_RS03075"
                     /old_locus_tag="ERS012477_00598"
     CDS             5001..5297
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /locus_tag="AP598_RS03075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00598"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000815394.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.5e-21,
                     bitscore: 68.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNGG
                     AYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            5374..7404
                     /locus_tag="AP598_RS03080"
                     /old_locus_tag="ERS012477_00599"
     CDS             5374..7404
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /locus_tag="AP598_RS03080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00599"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000825263.1"
                     /transl_table=11
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMINQ
                     PVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSKES
                     AETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLLSLA
                     IFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSLLGLG
                     ILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGKLPLKR
                     MMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGWSSAFAD
                     EEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTPSGNVIYV
                     SPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYLQNFSSESV
                     EVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQATGARPVAGML
                     WGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRVEFYSLLIGTI
                     LTLSTAILLFDSMNLLYFEQFRRDLMIKRLAGMTIYELHGKYLLAQGGVLLLGLVLSSI
                     LTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            7406..8047
                     /locus_tag="AP598_RS03085"
                     /old_locus_tag="ERS012477_00600"
     CDS             7406..8047
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /locus_tag="AP598_RS03085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00600"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000565531.1"
                     /transl_table=11
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLLN
                     ILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQ
                     KISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPT
                     AALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            8040..8333
                     /locus_tag="AP598_RS03090"
                     /old_locus_tag="ERS012477_00601"
     CDS             8040..8333
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /locus_tag="AP598_RS03090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00601"
                     /product="hypothetical protein"
                     /protein_id="WP_000333255.1"
                     /transl_table=11
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYLL
                     DFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            8584..9456
                     /gene="rsmA"
                     /locus_tag="AP598_RS03095"
                     /old_locus_tag="ERS012477_00602"
     CDS             8584..9456
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /locus_tag="AP598_RS03095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00602"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_001216863.1"
                     /transl_table=11
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     DQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKV
                     DLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRISAQ
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFF
                     FKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGL
                     ADALKGQGL"
ORIGIN
        1 aatggggatg aggtcttagt gccaatgcca gactatcctc tatggacggc ggctgtcagc
       61 ctagctggag gaaatgccgt tcactatatc tgtgatgaag ctgtggaatg gtacccagat
      121 attgacgata tcaagtcaaa aatcacttcc aataccaagg caatcgtcct tatcaatcca
      181 aataacccaa ctggagccct ttatcctaag gaactcttgc tggagattat tgagattgcc
      241 cgtcaaaacg atttgattat ctttgcggat gaaatctatg accgcatggt aatggacgga
      301 catgtgcata cgcctgtggc gagcttggca ccagatgtct tctgtgtcag catgaatggt
      361 ctgtcaaaat cccaccgcat agcaggtttc cgtgtgggtt ggatggtctt gtctggccct
      421 aagactcatg ttaagggcta tatcgaaggg ctcaatatgc tgtccaatat gcgcctttgc
      481 tctaacgttt tggcccaaca agtcgtacaa acttccttgg ggggtcacca atcagtcgat
      541 gaattgcttc ttcctggtgg acgaatctac gagcaaagaa atttcatcta taatgccatt
      601 caagatattc caggtttgtc tgccgttaaa cccaaggcgg ggctctatat cttcccaaaa
      661 atcgaccgca atatgtaccg tatcgatgat gatgagcagt ttgtccttga tttcttgaag
      721 caggaaaagg ttctcttggt tcatggtcga ggctttaact ggcaggaacc agaccacttc
      781 cgtatcgttt accttcctcg tgttgatgag ttagcccaaa tccaagaaaa gatgactcgt
      841 ttcttgaaac agtatcgtag atagggcttg cattcgaaaa agctggaaac atttgcctag
      901 ggctattgac aaaagtgaaa gaatcagata gaatagtaaa gtatgtttag taactgatca
      961 ctttatagcc ggctaaacga atacaaaatc tatgaggagg tattcatcgt gaaacgtact
     1021 tatcaaccaa gtaaacttcg tcgtgcgcgc aaacacggat tccgtaaccg tatgtcaact
     1081 aaaaatggtc gtcgcgtatt ggcagctcgt cgtcgtaaag gacgcaaagt tttggctgca
     1141 taatccaaac gaattctatc aaaaatcagt aggaactcga gtctactgat ttttattttt
     1201 gtaaaaaagt tcagtagatg caaatggatt cggaagcgat gttatagtag attgaaacta
     1261 gaatagtaca cctctacttc taaaatattg ttagaaatcg atttgactgt cctgatcgat
     1321 ttgtcctgtt attattttat tttactataa agttgaagta gctggagatg gcacagcgac
     1381 aatcacattc ccagatggtt cagtagtgac gattctagga aaagatacag ttcaacaatc
     1441 tgcgaaaggt gaatctgtaa ctcaagaagc tacaccagag tataagctag aaaatacacc
     1501 aggtggagat aagggaggca atactggaag ctcagatgct aatgcgaatg aaggcggtgg
     1561 tagccaggcg ggtggatcag ctcacacagg ttcacaaaac tcagctcaat cacaagcttc
     1621 taagcaatta gctactgaaa aagaatcagc taaaaatgcc attgaaaaag cagccaagaa
     1681 caagcaggat gaaatcaaag gcgcaccgct ttctgataaa gaaaaagcag aacttttagc
     1741 aagagtggaa gcagaaaaac aagcagctct caaagagatt gaaaatgcga aaactatgga
     1801 agatgtgaag gaagcagaaa cgattggagt gcaagccatt gccatggtta cagttcctaa
     1861 gagaccagtg gctcctaatg ctgctcctaa gacaacaagt gcaccgcaag caactgcagg
     1921 aacaatgcaa gatgttacct accagtcacc tgctggcaaa caattaccta acacaggttc
     1981 agcatcaagt gcagcacttg ctagtcttgg tctagtggtg gcaacaagtg gttttgcttt
     2041 gctaggaaga aagactagac gtagaaaata gaacagctag aaaattctat tctctactta
     2101 aagttagatt ataaggggga ttttgagaag tcatcaatcc tagtgatggg tgagaaaagt
     2161 gagaacccaa gataatcaca tactttagct gaataggaat attctatcaa tgtagccaat
     2221 ctcttctgtc tctaactgtg gaataggaga tgggcaatat cggatagaaa agatagcaga
     2281 atagctctct attgaagaga ggaggggaaa ccgaaaaatt aggtgcccct cctctttttt
     2341 ggtataatag aaggatagaa aacgaggtta gaagagatga tttttgatac acatacacac
     2401 ttgaatgtag aagaatttgc aggtcgtgag gcagaagaaa ttgccttggc tgctgagatg
     2461 ggtgtgacac agatgaatat tgttggtttt gataaaccga cgattgagca tgccttggag
     2521 ttggtagatg agtatgagca gctctatgcg actattggtt ggcatcctac agaagctggt
     2581 acttatacag aggaagttga ggcttacttg ttggataagt taaaacattc caaggttgtg
     2641 gctttaggtg aaattggctt agactaccat tggatgacag cgcccaaaga ggtgcaggag
     2701 caggtttttc gccgtcagat tcagctatct aaggacttgg atttgccttt tgttgtccat
     2761 acccgtgatg cgctggaaga tacctatgag attatcaaga gtgagggcgt tggtcctcgt
     2821 ggtggtatca tgcattcatt ttcagggacg cttgagtggg cagagaagtt tgtggatctt
     2881 ggtatgacca tttccttctc aggagtggtg accttcaaga aggcaactga cctccaagaa
     2941 gcagctaaag agttaccttt ggacaagatg ttggtagaaa cagatgcgcc ttacttagca
     3001 cctgtaccca agcgtggtcg tgaaaataaa acagcctata ctcgctatgt ggtcgacttt
     3061 atcgctgact tgcgtggtat gacgacagaa gagctggcgg tagcaacgac tgcaaatgca
     3121 gaacgcattt ttggattgga cagcaagtaa tgaaagagag aatttcccaa gttatcgtgg
     3181 ttgaaggtcg tgatgatacg gtcaatctca agcgttattt cgatgtggag acctatgaga
     3241 ctcgaggttc tgccatcaat gaccaggaca tagagcggat tcagcgtcta caccaacgtc
     3301 atggagtcat tgtctttaca gacccagatt ttaatgggga gcggattcgg cgcatgatta
     3361 tgacggccat tccaacagtt cagcatgcct ttctcaagcg agatgaagct gttcccaagt
     3421 ccaagaccaa ggggcgttct ctgggaattg agcatgccag ctatgaagac ctgaaaacgg
     3481 ctctagctca ggtgacagaa caatttgaac atgagagtca gtttgacatt agtcgtagcg
     3541 atttgattcg ccttggtttt ctagcagggg cagacagccg taagcgtaga gaatatctcg
     3601 gagagactct ccgaatcggc tattctaacg gcaagcaact cctcaaacgc ctagagttgt
     3661 ttggggttac tttggcagaa gtggaagaag ctatgaaatc ttatgagtag gaaagatgtg
     3721 accgttacaa ttttttaagt ttcacagtat ttttcgaagc aggtagaaga ggaggcgtct
     3781 gatgttaatt ggtcaaaaaa ttaaagagat tcggatagaa aaaggaatta gtcgtccaga
     3841 tttttgtgga gatgagcaag aactgacagt tcgtcaactg tcgcgaattg aaagtggagc
     3901 ttcgcaaccg agtttgccca agttagccta tattgctcgc cggctaggag ttccagttta
     3961 tagccttatg ccggattttt cagctcttcc ttctgcttat ttagaattga aataccagat
     4021 tttacgtgaa ccaatctatg gtaaagaaga ggagtacgat aagaaggaag cgtgtttgga
     4081 agagatttat aaaacatact ttgataatct tcctaaagaa gaacaattag catgtgaagt
     4141 attgcaggcg tgtttggata cttctagaac tagaaggcct gaatatgcag agttaatact
     4201 tgaggaacat atgcctcaga ttatagaaaa agaagcttat tcaataaatg atatgttgtt
     4261 gattcgtttg tttttttatc aaatgctcat tagaaaagat cttgccaaat ttataaatca
     4321 aatcgaaaag ctaatgctct ttcttttgga acagaagaag gtaactcaaa tagagaatta
     4381 ctttataatt agagatactc ttatttcagg aatgtgttgt cttgaaaagg taggagtaac
     4441 tgattgtttt aatgattatc tatcgtgttt acaagaaatt atggataaaa ctcaagatta
     4501 tcaaaagaaa cctcttgtat ttatgttttt gtggaagcaa gcattaagag tagaaagaga
     4561 ttttagttta gctgaatcat tttatcagtc ttctaaaaca tttgcgaagc taattggaga
     4621 tggatttcta gtaaagaaat tgacagagga atggcaagag gatgtcaaaa aatatttata
     4681 aacatagtga atcagtgaca aaaatgtcct tgtcctcgta tcaaaacagt tctaaagttc
     4741 gtctttagaa ctgttttttt agatataagc taaaaataat atgaaatagt tagattttaa
     4801 ggacattgat gtccttgata gtgtgataaa gatggagtat aatgactata aaagttcatt
     4861 tcagggtgct attttgacaa tttagagaaa aacaaataat tttagaatat catttggtaa
     4921 tataatagta tcaacgttga taatggaaag aggagcaagt aactcaacaa tcttgtaatc
     4981 aaaaatagag gagtatcaat atgaaacaaa cagttaaaaa attagctctt gtagcgagca
     5041 ttgcagcaac attaggtgga agtgtagcag ttgcctctgc agccgttcaa tacccagaag
     5101 gtggtgtttg gacttatggt tcaggtaacg gtggtgctta ctcaaactac tatcaccctt
     5161 caaaatacca tagttcaaca gttgttagca gaaaaactgg ttcatctgac aagggatatg
     5221 ctggtgctgg agggacttct cgtgcatgga ttcgtacttc ttggggagag aaagttgcat
     5281 tctactataa tgtttagaat ggtatagccc tattgtttag ctttacttgg tcaccttctc
     5341 actaagtgac caagtaactt tttggaggaa atgatgaaac gtttatttat tttgatttca
     5401 atggtattgg tatcgcttta tatggtgata acttccgttg accatcgaga agagatttta
     5461 tttggtaact atccttctgt tgatgtgaca ggaatgatga taaatcaacc agtagctagt
     5521 cgggaagagg tgacagaggc tttgagtcac ttggcggtag agcacaatag tctcattgct
     5581 cgtcgaatcg ttgagccaaa tgaagctgga gaaacacgct ttacctatgc cacttatggt
     5641 gagggaaagc ttccagaagg tctgaccatt tcctccaagg agagtgcaga aacgagtgat
     5701 ttattagggt cttacttgat tgtgtcagga agtttggatg gagtgagctt acagaccacc
     5761 ttgaaagagc ttggttatca aggctttgtt tcgaatggag aagatccatt ttcgatagtc
     5821 ttactattga cggccacccc tatggtgcta ctgagtttag ctatttttct gctgaccttt
     5881 atgagtctga ccctgattta tcggatcaaa tcccttcgtc aggcagggat tcgcttaata
     5941 gctggtgaga gcttgtttgg agttgctctc agaccagtgt tagaagatgt gagacagctt
     6001 atctgctcag tgctggtatc cagtcttttg ggattgggga ttctctggta tcaaggtgcc
     6061 ttgtttatgg caacggtgca actggtcatc attgctcttc tactttatgg attgaccttg
     6121 gcagggattt ctaccttact aagtgtcgtc tatctacttg gtttacagga aaatagtctg
     6181 gtggatctat tgaaagggaa actccctctc aaacgtatga tgacattgat gatggtgggg
     6241 caactcttag ctgtattggt ggtcggatcg agtgcgacag ctctcctacc ccactaccgt
     6301 gaaatgcagg aaatggagag agctagcaat aaatggagcc agtcctcaga ccgttaccgt
     6361 ctatcctttg gttggtctag tgcatttgcc gatgaagaag gaacgcgtaa ggataatcgt
     6421 gagtggcaga catttactga agaacggtta gccaatacag actcttttta tattatgagc
     6481 aatgttgaca atttctcaga tggagcagaa gtggacctag atggcaatcg tctcagtgac
     6541 tacacaccgt cagggaatgt tatctatgtc tcaccacgct atctgataga agaaaagatt
     6601 accgtttctt cagagtttat ggacaagatg caaaacttgt ctgagggaga gtttgggctg
     6661 atcttgcctg agagcttgcg agagcagtct gtctactacc aaggattgtt tacagattac
     6721 ctgcaaaact tttcatctga aagtgtagaa gtgacgagtc agaaacacta cctcccacag
     6781 gtaaggctag cttttacaga aacaggacag gaacgtttcc tctataatga tgggtacaag
     6841 acaacacgcc agtacctaaa agatccgatt attgtagttc taacgccgca agcgactgga
     6901 gcaagacctg ttgcagggat gttgtgggga actacggcta atagtgcctt gaaactagat
     6961 cgatatggag acagcatcac agctctaaaa gagcaaggtc tgtatcacaa ggtttcttac
     7021 ttggtaaaaa gccagctatt ttttgccaag gtactaaatg acaaacgggt ggagttttac
     7081 tctctcctta ttgggacgat tttgaccctg tctacggcta tcttgttatt tgattccatg
     7141 aatcttctct attttgagca gttcagacgg gatcttatga ttaaacgtct tgctggtatg
     7201 acaatctatg agcttcatgg caagtattta ctggcgcaag gaggagttct cttgcttggc
     7261 ctagtcctat ctagtatttt gacaagagat ggtttgatta gcgctctagt tgtagctttg
     7321 tttacgctta acgccctctt gattttagta aggcaggaca aaaaagaaga agctggtagc
     7381 atggcagtat tgaaaggaaa ataagatgat tgatattcaa ggattggaaa agaaatttaa
     7441 tgaccgcgcg attttctctg gtttgaatct caagctggag aagggcaagg tttatgcctt
     7501 aatcggaaag agtggaagtg gaaagacgac gctgctgaat atcttgggaa agctagaaaa
     7561 gatagatggt ggaagggttc tctatcaggg gaaagattta aaaaccattc ccactcgtga
     7621 gtattttcga gaccagatgg gctatctctt tcaaaatttc ggcctcttag aaaaccaatc
     7681 aatcaaagaa aatttggatt tgggttttgt tggtcagaaa atctcaaaag tagaacgttt
     7741 ggaaaggcaa gtgggggctt tagaaaaagt taatctaggg tatttggatt tagaacaaaa
     7801 aatctatact ttatctgggg gagaggccca acgagttgcc cttgctaaga ctattttgaa
     7861 aaatccaccc ttgattttgg cagatgaacc aacagcagct cttgatcctg aaaattcaga
     7921 ggaggttatg aatctcttgg tggatttgaa agatgaaaat cgaattatca tcattgcgac
     7981 ccataatccc ctagtctgga ataaggctga tgaaatcatt gatatgagga aacttgctca
     8041 tgtgtgaaaa aatccgtatt cgcagggtat ctgattatcc tagtgccaga ggtggtttag
     8101 aagatatcct catcatggaa aatatgacca atcatctcct tttggttcaa atccgagtgc
     8161 atggctattt gcttgatttt gctagtattg aagggcaaag gcaaaagcat tatcgtttga
     8221 aaaatttacc tcagacggtt gaactgacag tggatgatgt ggaggaggat gtggatttga
     8281 ccctacccga aaatcgaagt tatcaagaag ctgatttttt tgatactcaa tgaaaatcaa
     8341 agagcaaact aggaagctag ccgcaggctg tacttgagta cggcaaggtg aagctgacgt
     8401 ggtttgaaga gattttcgaa gagtatgaac gtatgtttcg agagaagcgc taagtccctc
     8461 tttaaagatt tccaagacta tctttcttca tgaggaaaga tagttttttg gtatgatttt
     8521 cattcccaaa atacaagggg aatgtgttac aatagtagta acagataata gaaaagagaa
     8581 tagatgagaa ttgcagatta tagcgtgacc aaggcagtgt tggagcgtca cggttttacc
     8641 tttaaaaagt cctttgggca aaatttcttg acggatacca atatccttca aaaaattgtg
     8701 gatacggctg aaattgatga tcaggtcaat gtcatcgaaa tcgggccagg tattggtgcc
     8761 ttgacagaat ttttggctga gcgtgcagcc caagtcatgg cttttgagat tgaccaccgt
     8821 ttggtgccaa ttttggcaga tactctgcgt gattttgata atgtgaccgt agttaacgaa
     8881 gatattctca aggttgattt ggcgcaacat atccaaaatt ttaaaaatcc taacctgcca
     8941 atcaaggtag tggctaattt gccttactac atcacgacgc ctattctcat gcacttgatt
     9001 gagagtggca ttcctttttg tgagtttgtg gtcatgatgc agaaagaagt agcggaccgc
     9061 atttcagccc agcctaacac caaggcttac ggtagcttgt ctatcgccgt gcagtattac
     9121 atgacagcca aggttgcctt tatcgtgcct cgtacggtct ttgtgccagc gccaaatgtg
     9181 gattcagcca tcttgaaaat ggtgcgtcgt ccagagccag ccgtagcagt agaagatgag
     9241 aactttttct ttaaggtttc caaggctagt tttacccatc gccgcaagac cttgtggaat
     9301 aacttgacag gttactttgg taagactgaa gaggtcaagg acaagctgac caaggctttg
     9361 gaccaggcag gcttgtcacc aagtgtgcgt ggggaagctc tcagcttggc agaatttgcc
     9421 ggtctagcag acgcacttaa agggcaagga ctctaagatg cagggacaaa tcattaaagc
     9481 cttggcaggt ttctactatg tggagagtga tggccaggtt tatcaaacac gcgcgcgtgg
     9541 gaatttccgt aaaaaaggcc atacccctta tgttggggac tgggtagatt tctctgccga
     9601 ggaaaattca gaaggctata tcctcaaaat tcacgaacgg aaaaacagtc tggttcgtcc
     9661 gcctattgtc aatatcgatc aagctgtagt aatcatgtcc gtcaaggaac ctgattttaa
     9721 cagcaatttg ctggatcgtt tcttggttct tttggagcac aagggcatcc atcccattgt
     9781 ctatatttcc aaaatggatt tgttggaaga taggggagaa ctggattttt accagcagac
     9841 ctatggtgac atcggctatg actttgtgac cagtaaagag gaactcctgt ctttgttaac
     9901 aggcaaggtt acggtcttta tggggcagac aggtgttggg aagtcaactc ttctcaataa
     9961 aatcgcacca gacctcaatc ttgaaacggg agaaatttca gacagtctag gtcgcggtcg
    10021 ccataccact cgagctgtta gtttttacaa tctcaacggg ggtaaaatcg cagatacacc
    10081 aggattttca tccttggact atgaagtatc aagggctgaa gacctcaatc aggctttccc
    10141 agagattgct actgttagcc gagattgtaa gttccgtact tgtacccata cccatgagcc
    10201 gtcttgtgcc gtcaaaccag ctgttgaaga gggtgttatt gcaaccttcc gttttgacaa
    10261 ttacctgcaa ttccttagtg aaattgaaaa tcgtaga
//
