LOCUS       NZ_CFVJ01000001         6208 bp    DNA     linear   CON 04-AUG-2024
DEFINITION  Streptococcus pneumoniae strain 17F/2, whole genome shotgun
            sequence.
ACCESSION   NZ_CFVJ01000001
VERSION     NZ_CFVJ01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001326475.1-RS_2024_08_04
            Annotation Date                   :: 08/04/2024 01:25:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,087
            Genes (coding)                    :: 1,951
            CDSs (with protein)               :: 1,951
            Genes (RNA)                       :: 33
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 59 of 136
            Pseudo Genes (incomplete)         :: 74 of 136
            Pseudo Genes (internal stop)      :: 39 of 136
            Pseudo Genes (multiple problems)  :: 30 of 136
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:58:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6208
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CFVJ01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..6208
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..6208
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6208
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[920:1208](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      921..1208
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(176..>502)
                     /locus_tag="AP598_RS00005"
                     /pseudo=""
     CDS             complement(176..>502)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /locus_tag="AP598_RS00005"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DNA-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYT
                     LLEDAKNKKSYDRLAICYVPIGICTDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     misc_feature    581..597
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.88"
                     /tool="antismash"
     misc_feature    586..599
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.93"
                     /tool="antismash"
     misc_feature    complement(587..600)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.42"
                     /tool="antismash"
     misc_feature    complement(859..875)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.57"
                     /tool="antismash"
     gene            921..1208
                     /locus_tag="AP598_RS00010"
                     /old_locus_tag="ERS012477_00001"
     CDS             921..1208
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /locus_tag="AP598_RS00010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00001"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_001230165.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.3e-31,
                     bitscore: 102.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFGY
                     SDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            1260..3368
                     /locus_tag="AP598_RS00015"
                     /old_locus_tag="ERS012477_00002"
     CDS             1260..3368
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /locus_tag="AP598_RS00015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00002"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000724257.1"
                     /transl_table=11
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDLY
                     LKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLDFT
                     TDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEMDKD
                     RIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEM
                     KRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPMLWLSHF
                     LLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTALLIGVGR
                     SLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFYQDLVEKT
                     QAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTGIKKVILMP
                     ASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFPYSDKRKENL
                     SNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKLSDGNYLKFSS
                     IQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRDIITKKFMGWKL
                     VDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVLGLASRMEKRSLV
                     ELLKGGIL"
     gene            3365..4006
                     /locus_tag="AP598_RS00020"
                     /old_locus_tag="ERS012477_00003"
     CDS             3365..4006
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /locus_tag="AP598_RS00020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00003"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571330.1"
                     /transl_table=11
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLLN
                     LLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLMLAL
                     KYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLILADE
                     PTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     misc_feature    complement(4113..4127)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.38"
                     /tool="antismash"
     misc_feature    4200..4220
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.21"
                     /tool="antismash"
     gene            4221..5027
                     /locus_tag="AP598_RS00025"
                     /old_locus_tag="ERS012477_00004"
     CDS             4221..5027
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /locus_tag="AP598_RS00025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00004"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_001038474.1"
                     /transl_table=11
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVALN
                     PDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKADLA
                     ISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQETM
                     AKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNFEKEQ
                     DDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            5044..>5436
                     /locus_tag="AP598_RS00030"
                     /old_locus_tag="ERS012477_00005"
                     /pseudo=""
     CDS             5044..>5436
                     /GO_function="GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269502.1"
                     /locus_tag="AP598_RS00030"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ERS012477_00005"
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDFI
                     HDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVEIA
                     HQIGATTIAHGCTGKGNDQVEYQIAVA"
     gene            complement(5649..>5975)
                     /locus_tag="AP598_RS00035"
                     /pseudo=""
     CDS             complement(5649..>5975)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /locus_tag="AP598_RS00035"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DNA-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="KILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYT
                     LLEDAKNKKSYDRLAICYVPIGICTDNAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     misc_feature    6054..6070
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.88"
                     /tool="antismash"
     misc_feature    6059..6072
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.93"
                     /tool="antismash"
     misc_feature    complement(6060..6073)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.42"
                     /tool="antismash"
ORIGIN
        1 gtaaaagtca tactttctcg ttaaatcgtt cggcgattca tggtgacatg gacacatagt
       61 agtaaacaca ctatgtggct tggcagagct aaaaacttct ggcttaacaa tagctggtat
      121 taagaactaa cgaacaaata atatgtaaaa ggtcgaggag ctccctcgac tttttttata
      181 atttctttgg ttgataatgg gtctctactt cttttttgag atgagacagc atagaagttt
      241 cctcggtcag ctccagaagt gagaaacctt tttggataag tttagcatta tctgtacaaa
      301 tcccaatagg gacatagcag atagcaagcc tatcgtagct tttcttattc ttggcgtcct
      361 ccagtaaagt gtagcagatt tgcttacaca tgtttttatc ttgattgtat agatagagtg
      421 tggaaaggtt tagcaggatt gctattcgca agtcatataa atgttgatag tttttataga
      481 cttctaagcg ttgcaagatt ttctcggatt attgacaaat aaacggacat tctgatagac
      541 gattgcccta gcggcttagt gtaaacttta caaatttggc ttgtaatttt tttataaaat
      601 tcactttagt tacttgacag ttattatatt ttcgctcata atatagataa aaggatggga
      661 taggagttta aaaatttgaa agcgctatcc atattgttta ttggactgat tcttgagtaa
      721 tagttagagt gtgcttaata ctactctggt tgttcaagga cctgatacag aagcgcacca
      781 ttctcccggt taagagtaag ttcgttgtta aaaaatgaaa tgagatagaa ggaaggtgag
      841 tccaatggac tacgatactt gttgataaat tactatagct gtttttacgg aacagctctc
      901 gataatagga gaaataaatc atgagaaaat tagttaaaat tggtgttgca tctttaatgg
      961 tatgtggtat acttgctact acaaatgctt tagcagtatg ggttgagggt ggtcaatgga
     1021 actatggagt aggttggaca ggaacttttg gatattctga ttacttacat tctactcgat
     1081 atcatacagc aactgttaga catgggggta gaacctctaa ggattatgca aaacctgagg
     1141 catgggctag agcttccctc accaagattc cgccaacagg aatggaatat ttctatggat
     1201 ttgagtaaaa ttcttccctc tcctatttgg agagggaaaa ctgtagtgga ggcactttta
     1261 tgaaaaaaat cagtaatttc tgtatgttac tcctgcttct gtgtaccact ttttttgttt
     1321 ttaatgtaaa ctatacacga gaagtggttc ggattcaaga aatgggaaag actgtagatt
     1381 ctttggattt gtatttgaaa gatattaacg aacctgcagc gtctgttctt cgattttttg
     1441 aggatgtatc aaaggagtat aaagtctcca tcatcaaaac agacagtggt gatgaggtgg
     1501 tcaagtctgg tgtttttgat aaagatacct tcccctacca agagtttggg atttcttctc
     1561 ttgattttac cacagatggt gaaggagtct atagtaataa agaaatttcc aataaacttg
     1621 gtacgattcc gacctttcta aaagccaaac ctattcagct tatgactttt caaacctata
     1681 tcaaggatac atctcgtagt ttaaatggtc gctatacgat aacttctaca caagagatgg
     1741 acaaggatag gattgtacag aaatggagcg attttttcaa gatagaccag gctaccttgc
     1801 tagagccgac ctacaaaagt gcagtggaag tcataaatcg agatttgctt ttatctgcca
     1861 ttgtttttgt cttggctatt ttgcttcttg tgttagtgac agtgtatcaa ccgatgatgg
     1921 agatgaaaag agttggggta caaaaattac ttggttttca agatagggct gttttagctg
     1981 atgttgtaaa aggcaacctt tacctcctcc taggtggggc tcttgtgatc aatctaggcg
     2041 tgtttttctt gcttgattat aagccaaaag atttgtttcc tatgctgtgg ttgtctcatt
     2101 ttttgctgtt gcagctttat ctctttatca gttggttgac ttacctctta atccaaaaaa
     2161 tgacaatcag ctctctgctg aaaggttttt catctttcaa atttggtctt atcttcaatt
     2221 atgtgatgaa aatagggaca actattttac tgacggcctt actgattggg gtgggcagaa
     2281 gtttagaaca agaaaacaaa gaacttgctt atcagcaaca gtgggtaagt caaggtaatt
     2341 acctgacctt agaaaccttc aaactcaatg ataatctgtg gcaagaagag ctagcagggt
     2401 cagggaaatc tacagattat ttctatcgat tttatcagga tttggtagaa aaaacgcagg
     2461 cgggctatgt gcagagtagc agtcttcctg taaaaaattt tgtccaatca gaacagattc
     2521 agcaatatca gttaacagat acggtggatg tttactatgc caatcgcaat tttctaaaga
     2581 gcaagggatt caagctacca gataccggta ttaaaaaagt tattttgatg ccagcaagta
     2641 cgaaaggtga agaagataaa aatcagctct tggggaagtt aattgccttt cattcgatga
     2701 agtatgaaga gcagcaaaaa cgaacgatag aggagatgga tgtcgagatt gcctattatg
     2761 aaggagattg gtcatttttc ccatatagtg ataagcgaaa ggaaaatctc tccaatccaa
     2821 ttattagctt ggtcaatgat tctgatatga tgtgggatga gaaagcctcc ctgtcaacaa
     2881 ctggcttaaa taatccgatt aaaattgaaa atacggttca acatcaaaaa gagattacag
     2941 agttagttga gaaattgtca gatggaaatt atttaaaatt ttcatctatt caagccattc
     3001 aacaagagaa agtggattct tatagagatg ctgttcggaa ttttaaccta ctctttgctt
     3061 tgtttggtct ccttagcatg atgatttcct acttcttact agtaacaact ttcttattga
     3121 agcgcaggga tatcattacc aagaagttta tggggtggaa actggtcgat cgctaccgtc
     3181 ctctcctcgt tctgctcttg ctgggctata gtttccctct tctagtcttg attttctttg
     3241 cccatgcgtt cttaccactt ctactgtttg caggttttac atgtctggat atactatttg
     3301 tgctaggctt agcttctagg atggagaaaa gaagtctagt agagttattg aaagggggca
     3361 tcttatgatt gagttgaaaa atattaccaa aaccattggg ggaaaagtga ttttggataa
     3421 cttatctctc aggattgatc agggggattt ggtagctatt gttggtaaga gtggtagtgg
     3481 gaagtcgacc ttgttaaatt tattgggttt gatagatggt gattatagcg gacggtatga
     3541 gatttttggt cagacaaatc tagcggttaa ttctgctaag tcgcaaacaa taatccgtga
     3601 acatatctct tatctgtttc aaaattttgc cctgattgat gatgaaacgg tcgagtacaa
     3661 tctcatgctg gcgctgaaat atgtgaaatt gcctaagaaa gacaagctca aaaaggtgga
     3721 agagatttta gagagagtag gtttgtcagc tactttgcat caaagggtct ccgagttgtc
     3781 tgggggcgaa caacaacgaa ttgcagttgc tagagccatc ttaaaaccca gccagctgat
     3841 tttagccgat gaacctacag gttcgctgga tcctgaaaat agagatttgg tcttgaagtt
     3901 tctcttagag atgaatcgag aagggaaaac agtcattatt gtgacccacg atgcttatgt
     3961 agcccaacaa tgtcatcgtg tcattgaatt gggcgaggga aaatgagttc attcagctcc
     4021 ttttgactgg ctgaatactc atgttttcca gagaaaaata gcataaatac gcctaggaat
     4081 gacattttat gtagcatttc taggtttttt ttgtttcaaa ttgaaaattt tttcaattta
     4141 ggcttgacaa aggatgagta tatgagtata ggagtattat ttatacaata aaaaagaata
     4201 aacataaaga aggctttgtt atgaataaga tgaagaaggt gttgatgacg atgtttggtt
     4261 tagtgatgct ccccctacta tttgcttgta gtaacaatca atcggctgga attgaagcca
     4321 tcaagtccaa aggaaaattg gttgtagccc tcaatccaga ttttgctcca tttgaatatc
     4381 aaaaagtggt tgatgggaaa aatcagattg tgggttcaga tatcgaatta gccaaggcta
     4441 tcgcaacaga actaggtgtc gaattggaac tatctcctat gagttttgac aatgtactgg
     4501 ctagcgttca atcaggaaaa gctgaccttg ccatatcagg tgtttctaag acagatgaac
     4561 ggagcaaggt gtttgatttt tcaactccct actatactgc aaaaaataaa ctcattgtca
     4621 aaaaatctga tttagccact tatcagtctg tcaacgattt ggcgcagaaa aaggtcggag
     4681 cgcagaaagg ttcgattcaa gagacgatgg cgaaagattt gctacaaaat tcttccctcg
     4741 tatctctgcc taaaaatggg aatttaatca cagatttaaa atcagggcaa gtggatgccg
     4801 ttatttttga agaacccgtt gccaagggat ttgtggaaaa taatcctgat ttagcaatcg
     4861 cagacctcaa ttttgaaaaa gagcaagatg attcctatgc ggtagccatg aaaaaagata
     4921 gcaaggaatt gaaagaggca gtcgataaaa ccattcaaaa gttgaaggag tctggggaat
     4981 tagacaaact cattgaggat gcctttaaag cgtccattga aaaatagaaa gaaggaaaag
     5041 aacatgagta aagaaaaagt aattttggcc tattctggcg gtttagacac atcagttgct
     5101 attacctggc tgaagaaaga ctatgatgtt gttgcagttt gtatggatgt gggtgaaggg
     5161 aaagacttag atttcatcca tgataaggct cttaaggttg gggcagtcga atcttatgtc
     5221 attgatgtta aggacgaatt tgctacagat tatgtgctag tggctcatca gtcacatgcc
     5281 tattatgaac aaaagtatcc cttggtatct gccttgagcc gccctctgat ttctaaaaaa
     5341 ttggttgaaa tagcgcatca gataggagcg actacaattg ctcatggttg taccggtaag
     5401 gggaatgacc aagtcgaata ccagattgca gtcgctaaga aagcgaatga agctaaaaag
     5461 tgatggagtg atgggttcag aataagaggt tggtagatta tcagcctctt tcttgtcgtt
     5521 gaaaaagtga gatatattga cttttgaaaa aaatgtcata attgagttgg gataaacgta
     5581 aaagtcatac tttctcgtta aatcgttcgg cgattctatg aaaaggtcga ggagctccct
     5641 cggctttttt atctttcctt cgcttggtaa tagatctcta cttctttttt gagatgagac
     5701 agcatagaag tttcctcggt cagctccaga agtgagaaac ctttttggat aagtttagca
     5761 ttatctgtac aaatcccaat agggacatag cagatagcaa gcctatcgta gcttttctta
     5821 ttcttggcgt cctccagtaa agtgtagcag atttgcttac acatgttttt atcttgattg
     5881 tatagataga gtgtggaaag gtttagcagg attgctattc gcaagtcata taaatgttga
     5941 tagtttttat agacttctaa gcgttgcaag attttctcgg attattgaca aataaacgga
     6001 cattctgata gacgattgcc ctagcggctt agtgtaaact ttacaaattt ggcttgtaat
     6061 ttttttataa aattcacttt agttacttga cagttattat attttcgctc ataatataga
     6121 taaaaggatg ggataggagt ttaaaaattt gaaagcgcta tccataatca taacgcataa
     6181 ttttctcctt ttcgtgaaaa aagttaac
//
