LOCUS       NZ_CP011522            15503 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Streptomyces sp. CFMR 7 strain CFMR-7 chromosome, complete genome.
ACCESSION   NZ_CP011522
VERSION     NZ_CP011522.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. CFMR 7
  ORGANISM  Streptomyces sp. CFMR 7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8207742)
  AUTHORS   Sudesh,K.
  TITLE     Complete genome sequence of Streptomyces sp strain CFMR 7, a natural
            rubber degrading Actinomycetes isolated from Penang, Malaysia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8207742)
  AUTHORS   Sudesh,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2015) School of Biological Sciences, University
            Science Malaysia, Minden, Gelugor, Georgetown, Penang 11800,
            Malaysia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Coverage                   :: 147x
            Sequencing Technology             :: PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001278095.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 02:27:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,124
            CDSs (total)                      :: 7,038
            Genes (coding)                    :: 6,918
            CDSs (with protein)               :: 6,918
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 35 of 120
            Pseudo Genes (incomplete)         :: 96 of 120
            Pseudo Genes (internal stop)      :: 7 of 120
            Pseudo Genes (multiple problems)  :: 16 of 120
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:05:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4682785
            Orig. end                         :: 4698288
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011522.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..15503
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /candidate_cluster_numbers="3"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /product="ectoine"
                     /region_number="24"
                     /rules="AfsA"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..15503
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /detection_rules="ectoine_synt"
                     /kind="neighbouring"
                     /product="butyrolactone"
                     /product="ectoine"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     cand_cluster    1..10966
                     /candidate_cluster_number="2"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10966
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5966](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5966
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(620..633)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.74"
                     /tool="antismash"
     misc_feature    687..700
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     gene            738..1217
                     /locus_tag="ABE83_RS19675"
                     /old_locus_tag="ABE83_19720"
     CDS             738..1217
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1240: riboflavin biosynthesis protein RibD"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763177.1"
                     /locus_tag="ABE83_RS19675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19720"
                     /product="nucleoside deaminase"
                     /protein_id="WP_019763177.1"
                     /transl_table=11
                     /translation="MGTHDSELMGFAHEAIRLSREHVAQGGIPFSGVVVGAGRILGTGF
                     NRVREDNDPTAHAEVVALREATTRHGIRAVAGATLIASGEPCALCYMAARFAGIGHIVH
                     TADRRTAARYGFDYTSTYSLFAEDPERWPIKVTALQLPEAEAPFQDFLTMSRTRH"
     gene            1383..1703
                     /locus_tag="ABE83_RS19680"
                     /old_locus_tag="ABE83_19725"
     CDS             1383..1703
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967532.1"
                     /locus_tag="ABE83_RS19680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19725"
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_019763176.1"
                     /transl_table=11
                     /translation="MHWLYLAIAVAFEIAVAIAAGKAEGFKNRKWTAITLASGAAATFF
                     LSKALLTFDVGVGYALWTSVAGVGITILGALFFGQRLNWNKAIGIVLVIGGVVGLQLSG
                     SA"
     gene            1785..2201
                     /locus_tag="ABE83_RS19685"
                     /old_locus_tag="ABE83_19730"
     CDS             1785..2201
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763175.1"
                     /locus_tag="ABE83_RS19685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19730"
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_053560537.1"
                     /transl_table=11
                     /translation="MSNAVKNPTNAWLSLLLAGVFEIGYALAVGGSEGFTVLTWSLVAV
                     VFFLLTLYALSLALRTIEVSIGYAVWAGIGAVGAAILGPVFFDETLTLAKGIWLTVIIV
                     GVIWLKLADRTKPEAAAPAPAAQQVTDRPGALQV"
     gene            complement(2339..3142)
                     /locus_tag="ABE83_RS19690"
                     /old_locus_tag="ABE83_19735"
     CDS             complement(2339..3142)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (22-192). E-value: 6.4e-15.
                     Score: 47.5. Matches aSDomain:
                     nrpspksdomains_ABE83_RS19690_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489554.1"
                     /locus_tag="ABE83_RS19690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19735"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_053560538.1"
                     /sec_met_domain="adh_short (E-value: 3.3e-38, bitscore:
                     130.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.9e-31, bitscore:
                     108.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPGGGPPSGRATPGDGLLSGKVALITGASSGIGAAAARHFADEG
                     AAVVLVARRAALLEEGAERIRKDGGRAVAVPGDVTRPADMERAVATAVDRFGRLDCAFN
                     NAGHASAGTVLHELPDEDFDRTLDVNLRGVWNCLRAQLPAMLAAGGGAVVNTSSVAGVR
                     ATAASAAYVAAKHAVIGLTRAAAAEYGEHGIRVNALVVGGTDTEMMDALRPPGPAVGAG
                     FGSRGIQRRLADPVEIARAAAWLCSDASSFTTGTALAVDGGRTAK"
     aSDomain        complement(2567..3076)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ABE83_RS19690_PKS_KR.1"
                     /evalue="6.40E-15"
                     /label="ABE83_RS19690_PKS_KR.1"
                     /locus_tag="ABE83_RS19690"
                     /protein_end="192"
                     /protein_start="22"
                     /score="47.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALITGASSGIGAAAARHFADEGAAVVLVARRAALLEEGAERIRK
                     DGGRAVAVPGDVTRPADMERAVATAVDRFGRLDCAFNNAGHASAGTVLHELPDEDFDRT
                     LDVNLRGVWNCLRAQLPAMLAAGGGAVVNTSSVAGVRATAASAAYVAAKHAVIGLTRAA
                     AAEYGEH"
     CDS_motif       complement(2981..3070)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ABE83_RS19690_0001"
                     /evalue="6.20E-07"
                     /label="PKSI-KR_m1"
                     /locus_tag="ABE83_RS19690"
                     /protein_end="54"
                     /protein_start="24"
                     /score="22.1"
                     /tool="antismash"
                     /translation="LITGASSGIGAAAARHFADEGAAVVLVARR"
     gene            complement(3139..3858)
                     /locus_tag="ABE83_RS19695"
                     /old_locus_tag="ABE83_19740"
     CDS             complement(3139..3858)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763173.1"
                     /locus_tag="ABE83_RS19695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19740"
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="WP_053560539.1"
                     /transl_table=11
                     /translation="MLSLRLAALNIDGVLLNDTFSPVIHQFVTSRGGTYSAALERSVFS
                     QPRHIAGRRLAAAVGGGMTGEAALAAYFEERAAHVDRHPVMITPGTRQLLHRLRSAGLR
                     TICYGGLGKEHFDHHLGRYAHLFDGPGYVCTNDFRPGLREITEDVFGLRPAQALFVDDV
                     ARVADAARELGTAFVGRPSAFVHSHQRALMREAGVRHLIDRLDTLDDGLLRRIDQEMNA
                     DTFWSASLTTAGRGGYA"
     misc_feature    complement(3847..3849)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4114..4746)
                     /locus_tag="ABE83_RS19700"
                     /old_locus_tag="ABE83_19745"
     CDS             complement(4114..4746)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452959.1"
                     /locus_tag="ABE83_RS19700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19745"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_029395135.1"
                     /transl_table=11
                     /translation="MAQPKQERAEQTRRALLHAAAEVFDEYGYAGASITRILKRAGVTA
                     GALYFHFGSKEDLAKAVMNSQPETLVPLVAAGGLQRLIDLTLVWSWQLQRDPLLRAGVR
                     LTNEQTSAEGPANADPYEKFRAIMTGYLQEAQEDGELQPGVDPAVVAEFVVAACTGMQM
                     YSNVVSGRRDLPERTQQMWNLILPGIAVPSVITRAEATPARGAAMMP"
     misc_feature    complement(4762..4777)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.84"
                     /tool="antismash"
     misc_feature    complement(4888..4905)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.91"
                     /tool="antismash"
     gene            5001..5966
                     /locus_tag="ABE83_RS19705"
                     /old_locus_tag="ABE83_19750"
     CDS             5001..5966
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489551.1"
                     /locus_tag="ABE83_RS19705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19750"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_053560540.1"
                     /sec_met_domain="AfsA (E-value: 6.1e-23, bitscore: 79.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRRDAQPVRQAPVRQAPVERPTVRRTQVHQWQVHKLRAEEVLLS
                     TWTEPCPGRFVITARWPKGHAFYRPSQGAYDPLLLAETVRQAVPLLSHAVHGVPREHKQ
                     AWEYLSFAVEPLASLVTTAEDEVRLVVTCSNAVRRGTRFAGMTMDIDVHLGKRLMGIAH
                     TRFNDQPAAIYRRLRGSYADVGAALEHCLPAGPPVEPHRVGRDSPADVVLSPALGGERP
                     GRWLLRVDPAHPVLFDHPVDHVPGMLLLEAARQAAHAVDGGDAGAVIGMRTDFVRYVEL
                     DAPAVVVAGTPVPAGTGRRRVPVSIEQHGVRVFTSEVTVA"
     cand_cluster    5129..15503
                     /candidate_cluster_number="3"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    5129..15503
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10128:10503](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(10129..10503)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="2"
     gene            complement(6224..6970)
                     /locus_tag="ABE83_RS19710"
                     /old_locus_tag="ABE83_19755"
     CDS             complement(6224..6970)
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: ACPS (132-191). E-value: 6.1e-09. Score:
                     27.7. Matches aSDomain:
                     nrpspksdomains_ABE83_RS19710_ACPS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763171.1"
                     /locus_tag="ABE83_RS19710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19755"
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="WP_019763171.1"
                     /transl_table=11
                     /translation="MTHDLKWSDAPPGEIRDQVAVWIVRAPAGQRLPPDGSPPDWLDEK
                     ERSRLASIPQHSGKEAYAFAHWALRSVLGSSLSCPPRSVGFLRQPCPGCGGAHGRPAIR
                     DPDRGGEPPEFSMSHSGDHVAIAVAEATIGIDIESHPTPESVAGYLPFLHPREQQWLGG
                     RPDDELVRDFARLWTRKEAMAKATGQGMAAVMNRLDLTGQPLGWRVRQLIAPPGYEASF
                     AVPASVHLEVTMHDELPYPMPELEQV"
     aSDomain        complement(6398..6574)
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ABE83_RS19710_ACPS.1"
                     /evalue="6.10E-09"
                     /label="ABE83_RS19710_ACPS.1"
                     /locus_tag="ABE83_RS19710"
                     /protein_end="191"
                     /protein_start="132"
                     /score="27.7"
                     /tool="antismash"
                     /translation="IGIDIESHPTPESVAGYLPFLHPREQQWLGGRPDDELVRDFARLW
                     TRKEAMAKATGQGM"
     gene            complement(7026..7268)
                     /locus_tag="ABE83_RS19715"
                     /old_locus_tag="ABE83_19760"
     CDS             complement(7026..7268)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489549.1"
                     /locus_tag="ABE83_RS19715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19760"
                     /product="DUF6253 family protein"
                     /protein_id="WP_019763170.1"
                     /transl_table=11
                     /translation="MSLLPAYGYQAAVQETCDTPEYHMPLVCWHDDDNGVRGLVLYRGA
                     LRPAEQVPGFRRYLAAEGLEQYATTGPWPLLAATA"
     gene            complement(7265..8080)
                     /locus_tag="ABE83_RS19720"
                     /old_locus_tag="ABE83_19765"
     CDS             complement(7265..8080)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763169.1"
                     /locus_tag="ABE83_RS19720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19765"
                     /product="maleate cis-trans isomerase"
                     /protein_id="WP_053560541.1"
                     /transl_table=11
                     /translation="MTQFQAREDGWGAVARVGVVVPHADVGPESELQAMAPRDVYIHGS
                     RVHFGAMRPGGEMDPKIPHDPVRAFIDPPFIDDAVELLAASPLDSIALGFTSSAYVLGA
                     DGEQKLFERLAERTRGIPLTGTTHAAAAGFRALGAERIALVNPPWFDDELSALGASYFG
                     ERGFDVVHHAPCGLPSNQKLITPEALVQWIESTVAPQQPQAVLVAGNGIRAVGTIAGLE
                     ERIGLPVLTANQVLFWHALHLAGGTEAAARISDYGALFGVRPEPGSVTG"
     gene            complement(8178..8897)
                     /locus_tag="ABE83_RS19725"
                     /old_locus_tag="ABE83_19770"
     CDS             complement(8178..8897)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489547.1"
                     /locus_tag="ABE83_RS19725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19770"
                     /product="transglutaminase family protein"
                     /protein_id="WP_078949746.1"
                     /transl_table=11
                     /translation="MTDTLTEAVPAPPAAEELDRLTAATDFLDHDHETVRAFVDKALQG
                     VDRATATPVELAVALYYAVRDGIHYEVYGADLSPQGLKASSIITGGKGFCLHKSVLYAA
                     ACRSVGIPARLHYSDVRNHLASDRLRSYIGGDVFFHGLATVYLDGQWLKVTPVFNKLLC
                     RLYGMTPLEFDGRSDSLYHPFDAQGRQSMEFLTDHGDFDDVPYEFVMGNMRRKHPKFLD
                     AEGTGTVSGGSLADEAS"
     gene            complement(8913..10043)
                     /locus_tag="ABE83_RS19730"
                     /old_locus_tag="ABE83_19775"
     CDS             complement(8913..10043)
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489546.1"
                     /locus_tag="ABE83_RS19730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19775"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_029395134.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 3.1e-17, bitscore:
                     61.9, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 4.1e-26, bitscore:
                     89.7, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 8.7e-33, bitscore:
                     112.3, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAEKDTDTYGITVPYGDELNDDVARWDRTGEFPHEKWKPLQRSG
                     LFTLPFAPEYGGAGRTLTETMAALEGLGHTSRDAGLNFSASTQIVSVGIPLQAFGSEEL
                     RSRHLPRVTSGEAITAHAITEPGHGSDVMNIRTTAVREGDEYVIDGGKMFITNAPIADL
                     FLLYVRTGKPGAFGLSTFLAERSTPGLKIGEPLETMGLRTSPISEVTLDNVRLPAANRL
                     GSEGAGFLIMDYVMKREVLFSFAVNLGEMTHRLKRVVEHATTRQQFGTAIGKNQAVSHK
                     IADMRIAVETARKWLYDTGRKVEQGTDASLDVAATKIVVSEANQRTAQHAIQIFGARGY
                     LTDTGIERELRNATSGSIYSGTNEIQRNCIAALMGL"
     gene            complement(10129..10503)
                     /locus_tag="ABE83_RS19735"
                     /old_locus_tag="ABE83_19780"
     CDS             complement(10129..10503)
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452967.1"
                     /locus_tag="ABE83_RS19735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19780"
                     /product="ectoine synthase"
                     /protein_id="WP_053560542.1"
                     /sec_met_domain="ectoine_synt (E-value: 8e-25, bitscore:
                     86.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSDVPAVEWGSGTSHRLLTEADGMGFTVCETLVRQGTTSG
                     LQYLGHLEACYCIGGSGEVVTTDGTRHKIKTGTIYALDQHDAHHLIASDDEDLLLVSVF
                     SPALRGDEKHSLSEDGFSRY"
     misc_feature    10579..10592
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.71"
                     /tool="antismash"
     gene            complement(10669..10929)
                     /locus_tag="ABE83_RS19740"
                     /old_locus_tag="ABE83_19785"
     CDS             complement(10669..10929)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763164.1"
                     /locus_tag="ABE83_RS19740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19785"
                     /product="acyl carrier protein"
                     /protein_id="WP_019763164.1"
                     /sec_met_domain="PP-binding (E-value: 5.9e-05, bitscore:
                     22.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTGANDPIRAYIVNEFLAGEDAEDLTDDFDLIESTVVNSLGLVR
                     LISWISETYHIPVDDIPLEPAAYRTLADIRSFVAQAAPASV"
     gene            complement(10926..12374)
                     /locus_tag="ABE83_RS19745"
                     /old_locus_tag="ABE83_19790"
     CDS             complement(10926..12374)
                     /NRPS_PKS="Domain: AMP-binding (1-382). E-value: 2.4e-58.
                     Score: 189.6. Matches aSDomain:
                     nrpspksdomains_ABE83_RS19745_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489543.1"
                     /locus_tag="ABE83_RS19745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19790"
                     /product="AMP-binding protein"
                     /protein_id="WP_078949747.1"
                     /sec_met_domain="AMP-binding (E-value: 4.3e-58, bitscore:
                     195.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQEQPDSTALVASGERVTYAELERLTRLAAADLDGAGFGFVRALC
                     VPAHKSPETIALLLAAWRQGINTLVPSPSLGAAALATLTAQAHGPLTATAVPGGGVILA
                     REETDPALADGFGVPDPAESLLTLTTSGSTGVPKLVPIRTGAFDRFADWATGKFGLDAA
                     TRALSFSPLNFDLALLDVWTVLEVGGTVVLADAGASGDAGYLRELALAHEVTLVQGVPL
                     LHRLLAADGARYPAVRTVVFTGEAVSEQGLRDAFAACPGARFHNVFGCTETNDSFIEDI
                     DPDAYVHPLPIGTPIDGTDAVLLIDGPGGTEVLQGPGTGELLVHTPFQTGGYLEQARNA
                     HAFMPDPRGGGAMFYRTGDLVHRDADGRYFLQGRTDFQVKVRGVRTNTLEVETVLGAHP
                     DVVEACVVALPCPEAGNRLHAEVVRRDGASLSSLGLRAYAAKHLPRHAVPSSVGLAGTP
                     LPKTPTGKPDRNSIKNNRLNGDAP"
     CDS_motif       complement(11199..11258)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ABE83_RS19745_0002"
                     /evalue="5.80E-06"
                     /label="NRPS-A_a8"
                     /locus_tag="ABE83_RS19745"
                     /protein_end="392"
                     /protein_start="372"
                     /score="18.9"
                     /tool="antismash"
                     /translation="GRTDFQVKVRGVRTNTLEVE"
     aSDomain        complement(11229..12371)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ABE83_RS19745_AMP-binding.1"
                     /evalue="2.40E-58"
                     /label="ABE83_RS19745_AMP-binding.1"
                     /locus_tag="ABE83_RS19745"
                     /protein_end="382"
                     /protein_start="1"
                     /score="189.6"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="QEQPDSTALVASGERVTYAELERLTRLAAADLDGAGFGFVRALCV
                     PAHKSPETIALLLAAWRQGINTLVPSPSLGAAALATLTAQAHGPLTATAVPGGGVILAR
                     EETDPALADGFGVPDPAESLLTLTTSGSTGVPKLVPIRTGAFDRFADWATGKFGLDAAT
                     RALSFSPLNFDLALLDVWTVLEVGGTVVLADAGASGDAGYLRELALAHEVTLVQGVPLL
                     HRLLAADGARYPAVRTVVFTGEAVSEQGLRDAFAACPGARFHNVFGCTETNDSFIEDID
                     PDAYVHPLPIGTPIDGTDAVLLIDGPGGTEVLQGPGTGELLVHTPFQTGGYLEQARNAH
                     AFMPDPRGGGAMFYRTGDLVHRDADGRYFLQGRTDFQVKVR"
     CDS_motif       complement(11949..11993)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ABE83_RS19745_0001"
                     /evalue="4.90E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="ABE83_RS19745"
                     /protein_end="142"
                     /protein_start="127"
                     /score="13.1"
                     /tool="antismash"
                     /translation="LTTSGSTGVPKLVPI"
     misc_feature    12579..12592
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     misc_feature    12610..12625
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            complement(12718..13161)
                     /locus_tag="ABE83_RS19750"
                     /old_locus_tag="ABE83_19795"
     CDS             complement(12718..13161)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489542.1"
                     /locus_tag="ABE83_RS19750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19795"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_018489542.1"
                     /transl_table=11
                     /translation="MTINQQPRAASDQTMWEAVLGLHAFVERQLAHTLQRRYGVGLSEY
                     RALETLHQAENGECRMQELADHIGLGQSSVTRLVGRLDSAGYAYKDSCTDDKRGVFAVI
                     TEEGRKRYQEARATYADVLSSALNTASADAQLARAVQALRSAA"
     misc_feature    complement(13199..13212)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.74"
                     /tool="antismash"
     misc_feature    13255..13268
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.24"
                     /tool="antismash"
     misc_feature    complement(13255..13270)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.67"
                     /tool="antismash"
     gene            complement(13285..14397)
                     /locus_tag="ABE83_RS19755"
                     /old_locus_tag="ABE83_19800"
     CDS             complement(13285..14397)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452971.1"
                     /locus_tag="ABE83_RS19755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19800"
                     /product="transposase"
                     /protein_id="WP_239009185.1"
                     /transl_table=11
                     /translation="MHETPEMATGRALDDLSRLLFGHLHRSDQQRWGQVYLQGLLRTHG
                     KKTVRRMAATVTGSEAAASSLHQFVNASPWEWTPVRGRLARWAARSLPVRSWTLAPTVL
                     PKRGDHSPGVHQRFLPESGRTVNCQLGLGLFLDAGQAHIPVDWQLFLPPRFTQDPAVRD
                     RAKIPATARPQPLRDHALHLVQEMTSWTEDGRTAPVVADLTALCDITPLAQRLQGSGHA
                     FVLAIPGQTALGGPELPPGERPSARALLLRRGVRHPAGAGRQIISYETPVREVRPSGEH
                     TQGAGRLLAEWDAGTGRPGRIWLTNITDRHPAELLELARSPRGAQSAVRRMGEDFGLLD
                     FEGRSFPGWHRHMTLVSAAYAYGALEGMTP"
     misc_feature    complement(14287..14289)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            14519..15301
                     /locus_tag="ABE83_RS19760"
                     /old_locus_tag="ABE83_19805"
     CDS             14519..15301
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452972.1"
                     /locus_tag="ABE83_RS19760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_19805"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_239009186.1"
                     /transl_table=11
                     /translation="MQERAARTRQTVLEAAADEFAERGYEGASLQRVAWRATTSIGALT
                     FHFRNKTALADEVRTTGRARFGRCLAELAPAADPLRELRTLIATLARLAHEDRFVRAAR
                     RLEADPPEGVPPLAETWLPVLRDLLDRAHGAHRLRPGVAPEDAVAMLAHLAEGAMAAQG
                     TPRDAAWDAVWDSVWDVVLHGLAADRGEGGGGGDHGGRGEGSERGDGGDQGGHVGQGVR
                     GDRSGRGVGDGRGEQGDRGAASGDASADMTGFARRGGG"
     misc_feature    14756..14758
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 gccgccgcgg cggtccgcgc ccgtgacgcc ggcttcgacg gcgtggagat ccacgcggcc
       61 aacggctatc tgctcgacca gttcctcacc ccgtacacca atctgcgcac cgacgggtac
      121 ggcggggacg ccgaggggcg ctcacggatc gtgcgggagg tgacggccga ggtcagaggc
      181 gcggccgggc ccggcttcac ggtcggcgtc cggttctccc aggggaagat caacgacttc
      241 gggtggcggt ggccgggcgg cgacgccgag gcgcgcgtcc tcttcacggc ggccgcctcg
      301 gccggtgccg actatctgca cctcgcgggc agcgggcggg actggtcgcc ggacgggccg
      361 acgctccccg ggctcgcccg gaaggtcacc gggctccccg tcatcgccaa cggcgctctg
      421 cacgaggacg cggtggcgcg gcgcgtcctg gacgaggggc acgccgacgt cctggcgatc
      481 gggacgggcg cgctggccaa ccccgacctg ccgcacaagg tggccgcggg ggcccttccg
      541 gcgccgttcg atccgcgggt gctggaaccg ctggcgacct tggacaacgc ccgtacgcac
      601 gcatccgctt gacaccagtt acatgcacat gcaagattaa gtccatcggg tcgcgtcaac
      661 ggcggagacc tgtggagcca tccgattgca tgtgcaggca aatgaacgat acagggcaac
      721 tcgggagacg aactgtcatg ggtacacacg acagcgaact gatgggcttc gcccacgaag
      781 cgatccggct cagccgcgag cacgtcgccc agggcggcat ccccttctcc ggtgtcgtcg
      841 tgggcgccgg gcggatcctg ggaaccggct tcaaccgggt ccgcgaggac aacgacccga
      901 ccgcgcacgc cgaggtcgtc gccctccgcg aggccaccac ccggcacggc atccgcgccg
      961 tggcgggggc caccctgatc gcctccggcg agccctgcgc cctgtgctac atggcggcgc
     1021 ggttcgccgg catcggccac atcgtgcaca ccgccgaccg ccggaccgcc gcccggtacg
     1081 gcttcgacta caccagcacg tactccctct tcgccgagga ccccgagcgc tggcccatca
     1141 aggtcaccgc gctccaactc cccgaggccg aggccccgtt ccaggacttc ctcaccatga
     1201 gccggacccg gcactgaccc aggcccgggg cccggaccgg gtcccgaacc gggacccggc
     1261 gccggccgga gcccccgggt gccgacccgg gccccggccc cgcgacatcg ccgtacaccg
     1321 ccgcacgcac cacctccccc acgctcgctc ccctcctcac ccaccgcacg gaaggacctc
     1381 ccatgcactg gctctacctc gccatcgccg tcgccttcga gatcgctgtc gccatcgccg
     1441 ccggaaaggc ggagggcttc aagaaccgca agtggaccgc catcaccctg gccagcggtg
     1501 ccgccgcgac cttcttcctc agcaaggcgc tgctgacctt cgacgtcggc gtcggctacg
     1561 ccctgtggac ctccgtcgcg ggcgtcggca tcaccatcct cggcgcgctc ttcttcggcc
     1621 agcgcctgaa ctggaacaag gcgatcggca tcgtcctcgt catcggtggc gtcgtcggcc
     1681 tccagctgag cggttccgcc tgagcccggg tgcaccaccc ccggccgcgc acgcggcgct
     1741 ccccgcacac catctgacgc catttcagag aggatccacc tcccatgtcc aacgcggtca
     1801 agaaccccac caacgcctgg ctctcgctcc tgctcgccgg agtcttcgag atcggatacg
     1861 cgctcgccgt cggcggcagc gaaggcttca ccgtcctgac ctggtccctg gtcgccgtgg
     1921 tgttcttcct gctgacgctc tacgcactga gcctggccct gcgcacgatc gaggtcagca
     1981 tcggctacgc ggtctgggcg ggcatcggcg cggtcggcgc cgccatcctc ggcccggtct
     2041 tcttcgacga gaccctcacc ctcgccaagg gcatctggct gacggtcatc atcgtcggtg
     2101 tcatctggct gaagctggcc gaccgcacga agcccgaggc ggccgccccg gccccggcgg
     2161 cccagcaggt cacggaccgg cccggcgcgc tccaggtctg acgcgcctcg accgtaacca
     2221 ccacggccgt agccatcaca accgtacgca cgacgaccgt acgcccccgg cggctgcctc
     2281 ccatcaggaa tgggcggcag ccgccggggg cgttccgtca tgtcgcttgc cacggcggtc
     2341 acttggcggt gcggccgccg tccaccgcga gggcggtccc ggtggtgaag gaggaggcgt
     2401 ccgagcagag ccaggcggcg gcacgggcga tctcgaccgg gtcggccagc cgtcgctgga
     2461 tgccccggga gccgaagccc gcgcccacgg cggggccggg cgggcggagg gcgtccatca
     2521 tctccgtgtc ggtgccgccc accacgaggg cgttgacccg gatgccgtgc tcgccgtact
     2581 cggcggccgc cgccctggtg aggccgatga ccgcgtgctt cgcggccaca taggcggccg
     2641 acgcggcggt ggcccggacg cccgcgacgc tggaggtgtt gacgaccgcg cccccgcccg
     2701 ccgccagcat ggcggggagc tgggcgcgca ggcagttcca caccccgcgc agattgacgt
     2761 ccagggtgcg gtcgaagtcc tcgtcgggga gttcgtgcag gaccgttccg gcgctggcgt
     2821 gaccggcgtt gttgaacgcg cagtcgaggc ggccgaaccg gtcgaccgcc gtggccacgg
     2881 cccgttccat gtccgcgggg cgggtgacgt cgcccggtac ggccacggcc cggccgccgt
     2941 ccttccggat gcgctcggcg ccctcctcca gcagggcggc ccggcgggcc accagcacga
     3001 cggcggcccc ctcgtcggcg aagtgccggg ccgccgcggc gccgatcccg ctggaggcgc
     3061 cggtgatcag cgccaccttg ccggacagca gcccgtcgcc cggcgtcgct ctgccggacg
     3121 gcggtcctcc gcccggcgtc acgcgtaccc gccccggccc gcggtggtca gggaggcgct
     3181 ccagaacgtg tccgcgttca tctcctggtc gatccggcgc agcagcccgt cgtcgagcgt
     3241 gtccagccgg tcgatcaggt ggcgtacgcc cgcctcccgc atgagcgcgc gctggtggct
     3301 gtggacgaag gcggaggggc ggccgacgaa ggcggtgccc aactcgcggg ccgcgtccgc
     3361 gacccgggcg acgtcgtcga cgaagagggc ctgggcgggg cggagcccga agacgtcctc
     3421 ggtgatctcc cggaggccgg ggcggaagtc gttggtgcag acatagccgg ggccgtcgaa
     3481 gaggtgggcg tagcggccca gatggtggtc gaagtgctcc ttgcccagac cgccgtagca
     3541 gatcgtgcgc aggcccgcgc tccgcagccg gtggaggagc tggcgggtgc ccggcgtgat
     3601 catgaccggg tggcggtcga cgtgggcggc gcgctcctcg aagtaggcgg cgagggccgc
     3661 ctcgcccgtc atgccgccgc ccacggcggc cgcgagccgc cggccggcta tgtgccgcgg
     3721 ctgggagaag acggatcgct ccagggcggc ggagtacgta ccgcccctgc tggtgacgaa
     3781 ctggtggatg accggactga aggtgtcgtt gagcaggaca ccgtcgatgt tcagcgcggc
     3841 caggcgtaag gaaagcatgc ggctccgtag ggatcgtccg gggcgggagg aacggtcgtc
     3901 gggcagagcg gtcggagggc ggtcggcgcg tgcgtggtcg gggcgtgtgt ggtcagggcg
     3961 ccctcggccg gggcgcccgt gatcaggaag ccctcggccg gggcctcggt gatcaggacg
     4021 cccgtggccg ggggccggtt cggccggtgc ggtcagggga gtcggtgcga tcggtcggtg
     4081 cgtgcgatcg gtccggtccg tgggccggtg cggtcagggc atcatcgcgg cgccccgggc
     4141 gggggtcgcc tcggcgcggg tgatcacgga cggcaccgcg atgcccggga ggatcaggtt
     4201 ccacatctgc tgggtgcgct cggggaggtc ccggcggccg ctgacgacgt tggagtacat
     4261 ctgcatcccg gtacaggcgg cgaccacgaa ctccgccacc acggccgggt cgacgcccgg
     4321 ctgcagctcg ccgtcctcct gggcctcctg cagatagccg gtcatgatcg cgcggaactt
     4381 ctcgtacggg tcggcgttgg cggggccctc cgcggaggtc tgctcgttgg tcagccgtac
     4441 gcctgcccgg agcagcggat cgcgctggag ctgccacgac cagacgagcg tgaggtcgat
     4501 gagccgctgc agcccgccgg cggcgaccag cgggaccagg gtctccggct ggctgttcat
     4561 caccgccttg gccaggtcct ccttggagcc gaagtggaag tagagcgccc cggccgtcac
     4621 accggcgcgc ttgaggatgc gggtgatgct ggctcccgcg tagccgtact cgtcgaagac
     4681 ctctgctgct gcatggagga gagcgcgacg ggtctgttcc gcccgctcct gtttcggttg
     4741 cgccatgccc ttcaccttcc cgaaaagaat gctccttatg ttacccggcc cgatcgccgt
     4801 caagttgagt gccttttcgc caagttgagg cagttatgcg ccccacttgc ctcagaggaa
     4861 acccggcacc cttaacccct tgacccagta aaacaaaaga aacattattt ttgggtcggt
     4921 gcatggagtg ccctgagcgc gcctctgtgt agtcgtccac gagcaactaa cgatctgggg
     4981 ggtccacgtt gtctgtttct gtgctgagac gcgatgcgca gccggtccgg caggcgcctg
     5041 tccggcaggc gccggtcgag cggccgacgg tccggcggac gcaggtgcac cagtggcagg
     5101 tgcacaagct ccgggcggag gaagtactgc tcagcacgtg gaccgagccg tgcccgggac
     5161 gcttcgtgat cacggcgcgc tggccgaagg ggcacgcgtt ctaccggccg tcgcagggcg
     5221 cgtacgaccc gctgctgctc gcggagaccg tacgccaggc ggtgccgctg ctctcccacg
     5281 ccgtgcacgg cgtcccccgg gagcacaaac aggcgtggga gtacttgagc ttcgcggtcg
     5341 agccgctggc ctcgctggtc accaccgccg aggacgaggt ccggctcgtc gtcacctgct
     5401 cgaacgcggt ccggcgcggc accaggttcg ccgggatgac catggacatc gatgtccacc
     5461 tggggaagcg gctgatgggc atcgcgcaca cccgcttcaa cgaccagccc gcggccatct
     5521 accgcaggct gcgcgggagt tacgcggacg tcggcgccgc actggagcac tgcctcccgg
     5581 cgggcccgcc ggtggagccg caccgggtgg gccgggactc cccggccgac gtggtcctct
     5641 caccggccct ggggggcgag agacccggcc ggtggctgct gcgggtcgac cccgcgcatc
     5701 cggtgctctt cgaccacccg gtcgaccatg tgcccgggat gctcctgctc gaagccgccc
     5761 ggcaggcggc gcacgccgtc gacggcggtg acgccggggc cgtcatcggg atgcgtacgg
     5821 acttcgtccg gtacgtcgaa ctcgacgccc ccgccgtcgt cgtggccggt accccggtcc
     5881 ccgccggtac ggggcggcgc cgcgtccccg tctccatcga gcagcacggc gtccgggtct
     5941 tcacctccga ggtgaccgtc gcctagggcg tgtgtcgaaa gtcccgcctg cccggcggtg
     6001 cccggcacgc acgctcgccg cgttgtcggg atcaccccga tacatccagt atcggggcga
     6061 ccctccgcct tgcgatcgca cgcaccggac accgccgggc ctgcccttcg ggcagacgac
     6121 gctactttcg acacacgccc tagggcccgc ccccggcgca ccgcacgacg cggcgggcgg
     6181 cccgtacctc ccctccacag aggtacggac cgcccgccac tggtcagacc tgctccaact
     6241 ccggcatcgg atacgggagt tcgtcgtgca tggtgacttc caggtgcacc gaggccggga
     6301 ccgcgaagct ggcctcgtac ccgggtgggg cgatcagctg ccgcacccgc cagccgagcg
     6361 gctggccggt gaggtccagc cggttcatga cggccgccat gccctggccc gtcgccttgg
     6421 ccatggcctc cttgcgggtc cacagccggg cgaagtcccg gaccagttcg tcgtccggac
     6481 ggccgcccag ccactgctgc tccctcgggt ggaggaaggg cagataaccg gcgaccgact
     6541 ccggggtcgg atgggactcg atgtcgatgc cgatggtggc ctcggcgacc gctatggcga
     6601 cgtggtcccc gctgtgcgac atcgagaact ccgggggctc gccgccccgg tccggatccc
     6661 ggatggccgg tcggccgtgc gccccgccgc agccgggaca cggctgccgc aggaagccga
     6721 cgctccgggg cgggcagctg agcgaggagc cgaggacgct ccgcagcgcc cagtgggcga
     6781 aggcgtaggc ctccttgccg gagtgctgcg ggatcgaggc cagccggctc cgttccttct
     6841 cgtcgagcca gtcgggcggg gacccgtcgg gcggcagccg ctgcccggcc ggggcccgga
     6901 cgatccagac ggccacctgg tcgcggatct cgcccggagg cgcgtcggac cacttgaggt
     6961 cgtgtgtcat gtcgatgcgg ctcctgatgt ggcggggacc cggccggtcc ccgccacggg
     7021 tcggatcagg cggttgctgc gagcagcggc cacgggccgg tcgtggcgta ctgctccaga
     7081 ccctcggcgg cgagatagcg ccggaacccc ggtacctgct ccgccgggcg cagcgccccg
     7141 cggtagagca ccaggccgcg cacgccgttg tcgtcgtcgt gccagcacac caggggcatg
     7201 tggtactccg gggtgtcaca ggtctcctgc acggcggcct ggtagccgta cgcgggcagc
     7261 aggctcatcc ggtcacgctc ccgggttcgg ggcggacgcc gaacagggcg ccgtagtcgc
     7321 tgatccgcgc ggccgcctcg gtgccgcccg ccaggtgcag cgcgtgccag aagagcacct
     7381 ggttggcggt gaggacgggg aggccgatcc gctcctccag gcccgcgatg gtgcccacgg
     7441 cgcggatgcc gttcccggcg accaggaccg cctgcggctg ctgcggggcg acggtggact
     7501 cgatccactg caccagggcc tccggggtga tgagcttctg gttgctgggg aggccgcagg
     7561 gggcgtggtg gacgacgtcg aacccccgct caccgaagta cgaggcgccc agggccgaca
     7621 gctcgtcgtc gaaccacggc gggttgacca gcgcgatccg ctcggcgccc agcgcgcgga
     7681 acccggccgc ggccgcgtgg gtggtgccgg tgagcgggat gccacgggtc cgctccgcga
     7741 gccgctcgaa gagcttctgc tcgccgtcgg cgcccaggac gtaggcggag ctggtgaacc
     7801 ccagggcgat ggagtccagc ggggaggccg cgagcagctc gaccgcgtcg tcgatgaagg
     7861 gcgggtcgat gaaggcgcgc accgggtcgt gcgggatctt ggggtccatc tcgccgccgg
     7921 gccgcatcgc cccgaagtgg acgcgtgagc cgtggatgta gacatcgcgc ggggccatcg
     7981 cctggagctc ggactccggc ccgacgtccg cgtgcggaac gacgacaccg acgcgggcga
     8041 cggcgcccca gccgtcctct cgggcctgga actgcgtcat ggggtgcttc tcctcttctt
     8101 ctgccgtgct tctgctgaca tgacgtgacg tgcctgggtg cggggacgtg ccgttgtgcg
     8161 gagatacggc tcggggctca gctcgcctcg tcggccagcg agccgccgct caccgtgccg
     8221 gtgccctccg cgtcgaggaa cttcgggtgc ttgcggcgca tgttgcccat gacgaactcg
     8281 tacgggacgt cgtcgaagtc gccgtggtcg gtgaggaact ccatgctctg ccgcccctgc
     8341 gcgtcgaagg ggtggtacag gctgtcggag cggccgtcga actccagcgg ggtcatgccg
     8401 tacagccggc acagcagctt gttgaagacc ggggtcacct tcagccactg gccgtcgagg
     8461 tagacggtgg ccagaccgtg gaagaagacg tcgccgccga tgtaggagcg cagccggtcg
     8521 gaggcgaggt ggttgcggac gtcgctgtag tgcagccgcg ccgggatgcc gacggagcgg
     8581 caggcggccg cgtagagcac ggacttgtgc aggcagaagc ccttgccgcc ggtgatgatg
     8641 ctggacgcct tcagcccctg cggcgacagg tcggcgccgt acacctcgta gtggatgccg
     8701 tcgcgcaccg cgtagtagag ggcgacggcg agttcgaccg gggtggcggt ggcgcggtcg
     8761 accccctgga gggccttgtc gacgaaggcg cgtacggtct cgtggtcgtg gtcgaggaag
     8821 tcggtggccg cggtgaggcg gtcgagctcc tcggcggccg ggggcgcggg gacggcttcg
     8881 gtcagggtgt cggtcatggc ggatgcctct cgtcacaggc ccatcagggc ggcgatgcag
     8941 ttgcgctgga tctcgttggt gccggagtag atggagccgg aggtggcgtt gcgcagctcg
     9001 cgctcgatgc cggtgtcggt cagatagccg cgggcgccga agatctggat ggcgtgctgg
     9061 gcggtgcgct ggttggcctc gctgaccacg atcttggtgg cggccacgtc cagcgaggcg
     9121 tccgtgccct gctcgacctt gcgcccggtg tcgtacagcc acttgcgggc cgtctccacc
     9181 gcgatcctca tgtccgcgat cttgtgggag accgcctggt tcttgccgat ggcggtgccg
     9241 aactgctgcc gggtggtggc gtgttcgacg acgcgcttga gacggtgggt catctctccg
     9301 aggttgaccg cgaaggagaa gaggacctcc cgcttcatca cgtagtccat gatcaggaag
     9361 ccggcgccct cgctgccgag ccggttggcg gcgggcagcc ggacgttgtc gagcgtgacc
     9421 tcgctgatcg gggaggtgcg cagccccatc gtctccagcg gctcaccgat cttcaggccc
     9481 ggcgtggagc gctcggcgag gaaggtgctc agcccgaagg cgcccggctt gccggtgcgg
     9541 acgtagagca ggaagaggtc ggcgatgggg gcgttggtga tgaacatctt gccgccgtcg
     9601 atcacgtact cgtcgccctc gcggacggcc gtggtccgga tgttcatcac gtccgagccg
     9661 tggcccggct cggtgatcgc gtgcgcggtg atggcctcgc cggaggtgac ccgcggcaga
     9721 tggcgcgagc gcagctcctc ggagccgaac gcctggagcg ggatgccgac gctgacgatc
     9781 tgcgtggacg ccgagaagtt gaggcccgcg tcccggctgg tgtggccgag gccctccagc
     9841 gcggccatgg tctcggtgag cgtccggccg gcgccgccgt actccggggc gaaggggagg
     9901 gtgaagaggc cggagcgctg gagcggcttc cacttctcgt gcgggaactc gccggtccgg
     9961 tcccagcggg cgacgtcgtc gttcagctcg tcgccgtacg gcactgtgat gccgtaggtg
    10021 tctgtgtcct tctctgctgc catgagggct ctgccttgcc tttcgccggt cgcggggtgg
    10081 gccgggtgcg gggccgcgac ggcgcggccc cgcacccggc ggcggtgatc agtagcggga
    10141 gaagccgtcc tcggagagcg agtgcttctc gtcgccccgc agcgccgggc tgaagacgga
    10201 gacgaggagg aggtcctcgt cgtcggaggc gatcaggtgg tgggcgtcgt gctggtccag
    10261 ggcgtagatg gtcccggtct tgatcttgtg acgggtgccg tcggtggtga cgacctcgcc
    10321 cgagccgcct atgcagtagc aggcctccag gtggccgagg tactgcagcc cgctcgtggt
    10381 gccctggcgg accagcgtct cgcagacggt gaagcccata ccgtccgcct cggtgagcag
    10441 gcggtggctg gtgccgctgc cccactcgac cgcggggacg tcggagagag aacggacgat
    10501 catgggagta ctcctgtcat cgctgttgtc tgggctgttg tccggacgtg tgctggatct
    10561 gtgctggtgc gcctcgtgcg tatgcgtatg cgtatgtgcc tgtgcgtgcg tgtctctgtg
    10621 cgcgtggggg aggggcggta cggggatggg gcggtggggc tcgtggggtc agacggaggc
    10681 cggggcggcc tgcgcgacga agctgcggat gtccgcgagc gtgcggtagg cggccggctc
    10741 cagggggatg tcgtcgacgg ggatgtggta cgtctccgat atccaggaga tgaggcggac
    10801 caggccgagg ctgttgacga cggtgctctc gatgaggtcg aagtcgtcgg tcaggtcctc
    10861 ggcgtcctcg ccggcgagga actcgttgac gatgtaggcg cggatcgggt cgttggcacc
    10921 ggtgctcatg gggcgtctcc gttcagtcgg ttgttcttga tcgagttacg gtcgggcttg
    10981 ccggtcgggg tcttcggcag tggggtgccg gcgagcccga ccgagctggg cacggcgtgg
    11041 cgcggcaggt gcttcgccgc gtacgcccgc agtcccagcg agctgaggga ggccccgtcc
    11101 cggcgcacca cttcggcgtg cagccggttg ccggcctcgg ggcagggcag ggcgacgaca
    11161 caggcctcga cgacgtccgg gtgggcgccg aggacggtct cgacctccag cgtgttggtg
    11221 cgcacgccgc gcaccttgac ctggaagtcg gtacggccct ggaggaagta gcggccgtcg
    11281 gcgtcgcggt ggacgaggtc gccggtgcgg tagaacatgg cgccgccgcc gcgcgggtcg
    11341 ggcatgaacg cgtgggcgtt gcgggcctgt tcgaggtagc cgccggtctg gaagggggtg
    11401 tgcaccagca gttcgccggt gccgggcccc tggaggacct cggtgccccc gggcccgtcg
    11461 atcagcagca ccgcgtcggt gccgtcgatc ggggtgccga tgggcagcgg atggacgtac
    11521 gcgtccgggt cgatgtcctc gatgaagctg tcgttggtct cggtgcagcc gaagacgttg
    11581 tggaagcggg cgcccgggca ggcggcgaac gcgtcccgca gcccctgctc ggagacggcc
    11641 tccccggtga agacgaccgt acggaccgcc gggtagcggg ccccgtccgc ggccagcagc
    11701 cggtggagga ggggcacgcc ctggaccagg gtcacctcgt gggcgagggc cagctcccgc
    11761 agatagccgg cgtccccgct ggccccggcg tcggccagca cgaccgtgcc gccgacctcc
    11821 aggaccgtcc agacgtccag gagcgccagg tcgaagttga gtggcgagaa gctcagcgcc
    11881 cgggtggcgg cgtcgagccc gaactttccg gtggcccagt cggcgaaccg gtcgaaggcc
    11941 ccggtccgga tcgggacgag cttggggacg ccggtggacc cggaggtggt gagggtcagc
    12001 agcgactcgg cggggtcggg gacgccgaag ccgtccgcca gggcggggtc ggtctcctcc
    12061 cgcgccagga tcacgccgcc gccggggacc gcggtggccg tcagcgggcc gtgggcctgg
    12121 gcggtgagcg tggccagggc ggcggcgccc agactcgggg aggggaccag ggtgttgatc
    12181 ccctggcgcc aggccgccag cagcagggcg atggtctccg gcgacttgtg ggccggtacg
    12241 cacagggcgc ggacgaatcc gaagccggcg ccgtccaggt ccgcggcggc gaggcgggtc
    12301 agccgctcca gctccgcgta ggtgacccgt tcgccggagg cgacgagcgc ggtgctgtcg
    12361 ggctgttcct gcacatgtct gctgaatgcc tgtaacaggc gaggcgatgg caacggtccc
    12421 ccctcggtgg ttgggcgatc tggcggcagg atccggctgt gcggtccggc cgcgcgatcg
    12481 agctgcgcga gccgggtccg cgatcgggcc tgagccggac gagccgggcc gtgaggccgc
    12541 gagatctggc tacgagagag aatctacggg tctctatttg catggtcaag caaatagata
    12601 gagcgttcgt gtgatccagg ccacaccctc gcctacgtcg gaagaagccc gaacatgcaa
    12661 aagacggcgg gggcgccggg tcgccccggc cgcctcccgc cgtccgtcgt ggtcgtgtca
    12721 ggcggcgctg cgcagcgcct gcaccgcccg ggcgagctgg gcgtcggcgc tcgcggtgtt
    12781 gagggccgag ctgaggacgt cggcgtacgt ggcacgggct tcctggtagc gcttgcggcc
    12841 ttcttcggtg atgacggcga agacgccgcg cttgtcgtcg gtgcagctgt ccttgtaggc
    12901 gtatccggcg ctgtcgagcc gccccaccag gcgggtcacc gaactctggc ccaggccgat
    12961 gtggtccgcc agctcctgca tccggcactc gccgttctcg gcctggtgga gggtctccag
    13021 ggcccggtac tcggacaggc cgacgccgta gcggcgctgg agggtgtgcg cgagctggcg
    13081 ttccacgaag gcgtgcaggc cgaggacggc ctcccacatg gtctgatcgg aggcggcgcg
    13141 tggctgctgg ttgatcgtca tggaacagct ccccttctcg actgcgataa caacaagata
    13201 catgcccatg caatcatctg gggagtgccg aagcctgcaa ggcgggctct ggccgatatt
    13261 tatttgtatg cgcaagtagt tgcgtcaggg ggtcatgccc tccagggcgc cgtacgcgta
    13321 ggcggccgag acgagcgtca tgtgccggtg ccagccgggg aaggagcggc cctcgaagtc
    13381 gaggagcccg aagtcctcgc ccatccgccg gacggccgac tgcgcccccc ggggcgagcg
    13441 ggccagctcc agcagttcgg ccggatggcg gtcggtgatg ttcgtcagcc agatccggcc
    13501 cggacgcccg gtgcccgcgt cccactcggc cagcagccgg cccgccccct gggtgtgctc
    13561 accgctcggg cgcacctcgc gcacgggggt ctcgtacgag atgatctgcc ggcccgcgcc
    13621 cgccgggtgc cgcaccccgc ggcgcagcag cagcgcacgt gccgacggcc gttcgcccgg
    13681 cggtagttcg ggtccgccga gggcggtctg ccccgggatg gcgagtacga aggcgtgccc
    13741 gctgccctgg agccgctgtg ccagcggcgt gatgtcgcac agggccgtca ggtcggccac
    13801 caccggtgcg gtccgcccgt cctccgtcca ggacgtcatc tcctggacca ggtgcagggc
    13861 gtggtcccgc agcggctggg gccgggccgt cgcggggatc ttcgcccggt cccgtacggc
    13921 ggggtcctgg gtgaaccggg gaggcaggaa gagctgccag tcgaccggga tgtgcgcctg
    13981 tcccgcgtcc aggaaaaggc ccagccccag ttggcagttg acggtgcgtc cggactccgg
    14041 caggaagcgc tggtgcaccc cgggcgagtg gtcgccccgc ttgggcagca ccgtcggcgc
    14101 cagggtccag ctccgcaccg gcagcgaccg ggcggcccag cgggccagcc ggccgcggac
    14161 cggggtccac tcccaggggc tggcgttcac gaactggtgc agcgacgacg ccgccgcctc
    14221 ggagccggtc acggtggccg ccatgcggcg cacggtcttc ttgccgtgcg tgcgcagcag
    14281 cccctgtaag tacacctgcc cccaacgctg ctggtcggag cggtgcaaat ggccgaagag
    14341 gaggcgggac aggtcgtcca gcgctcggcc cgtcgccatc tcgggcgttt cgtgcatcgt
    14401 cgtcactgca ggtccctccc aggtgagcgt cgctgagcag actaacaaac atagagaatg
    14461 ttcgttatat ttctgaggga gaagcacgac gacttaccga ggttcaagga gaaggcgcat
    14521 gcaggaacgg gcggccagga cgcgccagac cgtgctggag gcggcggccg acgagttcgc
    14581 cgagcgcggt tacgagggcg cctccctcca gcgggtggcc tggcgggcca cgacctcgat
    14641 cggcgctctg accttccact tccgcaacaa gaccgccctg gccgacgagg tgcgcaccac
    14701 cgggcgcgcc cgcttcggcc gctgcctggc ggaactcgcc cccgccgccg acccgttacg
    14761 ggaactgcgc accctgatcg ccacgctggc ccggctggcg cacgaggacc gcttcgtccg
    14821 cgccgcacgc cgcctggagg ccgatccgcc ggagggcgta ccgccgttgg ccgagacctg
    14881 gctccccgtc ctccgcgacc tgctcgaccg ggcccacggc gcccaccgcc tccgccccgg
    14941 cgtcgccccc gaggatgcgg tggcgatgct ggcccacctc gcggagggcg cgatggccgc
    15001 ccagggcacc ccgcgcgacg cggcgtggga cgcggtctgg gactccgtct gggacgtggt
    15061 cctgcacggc cttgcggcgg accgggggga ggggggtggc gggggcgacc acggtgggcg
    15121 gggcgagggg agcgaacggg gcgatggagg cgatcagggc gggcatgtcg gccagggggt
    15181 ccggggtgac cggagtggcc ggggcgtcgg ggatggccgg ggggagcagg gcgatcgagg
    15241 ggcggcctcg ggggatgcct cggcggacat gaccgggttc gcgaggcggg gcgggggctg
    15301 atcggggcgg tcgtgcgccg ggcggggcct gaccgcatca gcggctcggg gccggtcccg
    15361 gcccgtccga cagccccggc ccaccccgcc ccgtccggcc ctcaccccgc ccgcagcgcc
    15421 cggtccaccg cgcccgccac ctcgtccagg agacgggcgc ccgggcccgc cgtcaggcgg
    15481 ccgcggatcc aggaggcgcc ggt
//
