LOCUS       NZ_CP011522            11407 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Streptomyces sp. CFMR 7 strain CFMR-7 chromosome, complete genome.
ACCESSION   NZ_CP011522
VERSION     NZ_CP011522.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. CFMR 7
  ORGANISM  Streptomyces sp. CFMR 7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8207742)
  AUTHORS   Sudesh,K.
  TITLE     Complete genome sequence of Streptomyces sp strain CFMR 7, a natural
            rubber degrading Actinomycetes isolated from Penang, Malaysia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8207742)
  AUTHORS   Sudesh,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2015) School of Biological Sciences, University
            Science Malaysia, Minden, Gelugor, Georgetown, Penang 11800,
            Malaysia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Coverage                   :: 147x
            Sequencing Technology             :: PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001278095.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 02:27:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,124
            CDSs (total)                      :: 7,038
            Genes (coding)                    :: 6,918
            CDSs (with protein)               :: 6,918
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 35 of 120
            Pseudo Genes (incomplete)         :: 96 of 120
            Pseudo Genes (internal stop)      :: 7 of 120
            Pseudo Genes (multiple problems)  :: 16 of 120
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:05:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1611797
            Orig. end                         :: 1623204
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011522.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11407
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="12"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11407
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11407
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6407](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6407
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(713..1489)
                     /locus_tag="ABE83_RS06295"
                     /old_locus_tag="ABE83_06310"
     CDS             complement(713..1489)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891630.1"
                     /locus_tag="ABE83_RS06295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_06310"
                     /product="restriction endonuclease"
                     /protein_id="WP_053558773.1"
                     /transl_table=11
                     /translation="MSARRRTPARRSPSRRSPSRRTPSRRRGRQQGRSWAVLAVGVLFA
                     LWVVGRLLDWVAANRWVVLLVLLLAVAGGVLWFRYRADRLRREQEWARTLRYAVEQLDR
                     LHHRAFEHAVRDLMFRDGCTDAVQVGGAGDHGADVKATDPYGRRWVIQCKHRKAGSAGA
                     PVGTPDLHVLNGTGRPVHHGDVVVLVTNGRFSRPALEFARSQRLHLVDRATLSAWAGGG
                     RPLWELLRAVPPPRSPGPSPSPSPNPSSSPSPTPLT"
     gene            complement(1802..1990)
                     /locus_tag="ABE83_RS06300"
                     /old_locus_tag="ABE83_06315"
     CDS             complement(1802..1990)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049036.1"
                     /locus_tag="ABE83_RS06300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_06315"
                     /product="hypothetical protein"
                     /protein_id="WP_029394939.1"
                     /transl_table=11
                     /translation="MDDDASYCTPPAATPPTGTGTAEPFAECVLCREPTEYPESTKGAT
                     LCPVCAWQEAGRSACSG"
     gene            complement(2017..2691)
                     /locus_tag="ABE83_RS06305"
                     /old_locus_tag="ABE83_06320"
     CDS             complement(2017..2691)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="ABE83_RS06305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_06320"
                     /product="AIM24 family protein"
                     /protein_id="WP_019762319.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHLAQPATAPGMTLQNSKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKSQGIGGLLKRAVTGEGLPLMAVRGQGEAWFAHEAANCFIVEMDQGDQLT
                     INGRNVLCFDATLAYEIRTVKGAGMVGGGLFNSVFSGYGKIGLMCEGTPIVIPVTAAQP
                     VYVDTDAVVGWSQHLTTTLHRSQSVGSMVRGGSGEAVQLMLQGEGFVIIRPSEARPERT
                     AN"
     gene            2763..3563
                     /locus_tag="ABE83_RS06310"
                     /old_locus_tag="ABE83_06325"
     CDS             2763..3563
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491671.1"
                     /locus_tag="ABE83_RS06310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_06325"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_053558774.1"
                     /transl_table=11
                     /translation="MSSDDTAQSPSGDISPSPFHEGPAGSADPAGSARPADPDAAPQYV
                     LPLVLHLEKTDPPARTDALRTAARAVLTILADERSTGEGEWAKAMRDWEDARIRKVVRR
                     ARGAEWRKAAALPGITVTGGSAEVRVYPPIPLDGWPKELAKLQVSGTDLDDPEPPAAPD
                     REGPVLWLNPELEMSAGKTMAQVGHGAQLAWWELSDTDRKAWREAGFPLSVATAPPDRW
                     RELITSGLPVVRDAGFTEIAPGPTVVAEGGERFCPLPGVRTARR"
     misc_feature    3763..3777
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     misc_feature    4072..4087
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            4101..4847
                     /locus_tag="ABE83_RS06315"
                     /old_locus_tag="ABE83_06330"
     CDS             4101..4847
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="ABE83_RS06315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_06330"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_018491672.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATVGALAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHADLDRRAR
                     DVAAKLGVELPNQPTEQQQAWLRELTAASGDEYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGFVDFDALAREAAGGSTASPSGPPAPREGQVPL
                     APVPVTPSGDQSFTSRPVPPTMPPMP"
     gene            5001..6407
                     /locus_tag="ABE83_RS06320"
                     /old_locus_tag="ABE83_06335"
     CDS             5001..6407
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491673.1"
                     /locus_tag="ABE83_RS06320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_06335"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_053558775.1"
                     /sec_met_domain="DUF692 (E-value: 4.1e-94, bitscore: 313.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGIGIGWRPEIAAEVEALTGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVSLGLGGADRPDPERLAGLAARAELLGTPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALISWPDEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNRGEDPAKALDELPVEAIAYVHVAGGVEKDGVWHDTHA
                     HPVTAPVLDVLAELRSRVDPPGVLLERDDAFPPGAELAAELETIRGTLRSAARAGAVAR
                     AAVPPSAPAAPAAAAPAAAVAPAASSALPTPSASSDPSADAREAGPLGAPRPGARDRTA
                     VAQTALLSALVAGTPAPEGFDHRRLRVQSRALAAKRADVVAKVAPELPEILGDGYRPAF
                     LSYARSRPMNGGYRRDAMEFVERILVSGGLPDPVAQRRLTHWWEDRSGPRPPGRTTRLV
                     RAARAVLVGK"
     gene            6416..7447
                     /locus_tag="ABE83_RS06325"
                     /old_locus_tag="ABE83_06340"
     CDS             6416..7447
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514514.1"
                     /locus_tag="ABE83_RS06325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_06340"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_053558776.1"
                     /transl_table=11
                     /translation="MNTFALVLTLAVVLSTALLIGKAVASRSGRRTPVGAPYPVRDLYE
                     AAFLNGGPARVADTALTTLYGDGRVLIGGPGIVSVRHEQAHDPVERAVFQVLATAPSGA
                     LHAVREAVMRHPAVQEIGDRLAERGLLVPPAASRPVRRWGLIQGVSCIVGFPLAIAATV
                     IQYVNHDSPFDMPFPFVLKVLPAILLGGISGLVVAAAAAGRLTRAGRRAAHEFRTAHAY
                     QVTPPYLVATQGLRALPDPVLQAHLIAAAGVNAPSGRSRHRYGTAGVVGGTAAAGGFAA
                     TTVWCADAGPGHTSCGGSSGGGSGSGCGGGSGCGGSGSSCGGGSSCGGGSSCGGGGGCG
                     GGS"
     gene            7711..9279
                     /locus_tag="ABE83_RS06330"
                     /old_locus_tag="ABE83_06345"
     CDS             7711..9279
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-289). E-value:
                     7.6e-16. Score: 50.4. Matches aSDomain:
                     nrpspksdomains_ABE83_RS06330_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="ABE83_RS06330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_06345"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_053558777.1"
                     /sec_met_domain="PF00561 (E-value: 1e-21, bitscore: 76.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVPLYATIGSLVLSALVGTAPAAASDRTGGGSAEERGTAVAAA
                     RAAAAGISFGACPEEEMFPDSLECGTVRVPLDYARPDGPAIPLAVSRTRATGEPAERQG
                     SFVYNPGGPGASSITFPLLGEMSEWKEVAEAYDLVGYAPRGVGASAPLSCQDPAVFAQS
                     PTDSPAHPTTAYKEKRIAQARAYVKGCEESAGEALRHYTSLNNARDLEVLRAALGDERL
                     TFMGASYGTYIGSLYATLFPARVRRMVFDSAVDPDPEKIWYRSNLDQSLAFESRWEDFR
                     RWVARHDDVYGLGASPEAVQRHYDDVRTALAAEPAGGTVGPGQLHTGFLQAVYYDDYWA
                     MRATALSEYVRGNPEPLVTQLSPRAVTAKDDENERAVYTAVECNDAPWPEEWEVWDRDH
                     TDLARTAPFQTWHNAFTNLPCAFWPAPRQRPLDVSTGWGELPPVLILAAERDAATPYKG
                     ALELQRRLRGAVLVTERGAGTHGVGGFGNACVDDHMRRYLLTGETPAGDAECAPRAEPN
                     PVSLD"
     aSDomain        8026..8577
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ABE83_RS06330_Abhydrolase_1.1"
                     /evalue="7.60E-16"
                     /label="ABE83_RS06330_Abhydrolase_1.1"
                     /locus_tag="ABE83_RS06330"
                     /protein_end="289"
                     /protein_start="105"
                     /score="50.4"
                     /tool="antismash"
                     /translation="FVYNPGGPGASSITFPLLGEMSEWKEVAEAYDLVGYAPRGVGASA
                     PLSCQDPAVFAQSPTDSPAHPTTAYKEKRIAQARAYVKGCEESAGEALRHYTSLNNARD
                     LEVLRAALGDERLTFMGASYGTYIGSLYATLFPARVRRMVFDSAVDPDPEKIWYRSNLD
                     QSLAFESRWEDFRRWVARHDD"
     gene            complement(9440..10168)
                     /gene="hemQ"
                     /locus_tag="ABE83_RS06335"
                     /old_locus_tag="ABE83_06350"
     CDS             complement(9440..10168)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="ABE83_RS06335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE83_06350"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_029395334.1"
                     /transl_table=11
                     /translation="MSAPETVTPSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRT
                     GYADEVQELFDQLAAKDVTVRGTYDLSGLRADADLMIWWHAETSDQLQDAYNLFRRTKL
                     GRALEPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EIPFYTGRRKDIADLVAGLA"
     misc_feature    11207..11221
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
ORIGIN
        1 tcgagcggcg cccggccacg ggaacaccgg cgggcggctc cgcgtccgac gagaaggtcg
       61 tggcgctctc cttcgacgcg gacatgacgg ccgaccaggg cccccgggcg aaggcgggcg
      121 aacgcttcga caacccggag ctgatcgcgc tcctgcgccg gctgaaggtg ccctccacgg
      181 tcttcatgac cgggcggtgg gcggaggagt acccggcgca ggcgaaggac atcggcgcgg
      241 acgacctgtt cgaggtcgcc aaccactcgt acagccacta cgccttcacc gccgactgct
      301 acggcctgcc ggtggtcgcg aaggagacca tggcgcgcga tgtggagcgg gccttcgccg
      361 ccttccgcga ggccggtgta cggaacgtgg tgccgtactt ccgcttcccc ggcggctgct
      421 acgacgacgc ggccctgcgc gcgctggccc cggcgaacgt gacggcggtg cagtgggacg
      481 tggtgagcgg cgacgccttc gcgacggacg cgggggcggt ggcccggcag gtgctggacg
      541 gggtgcggcc ggggtcgctg gtggtgctgc actgcacgcg cagcgcggca ccggtcaccg
      601 gcgatgcggt gggcaggatc gttccggagc tacggaagcg gggctaccgg ttcgtgaagg
      661 tgtcggagct gatggagggc tgacgggggc cggtcgagcc tccgccaaga cctcaggtca
      721 acggagtcgg actggggctg gaactggggt tgggactcgg gctggggctg gggccggggc
      781 tgcgcggagg cggcacggcc cgcaggagtt cccagagggg ccggccgccc ccggcccagg
      841 cggagagcgt ggcgcggtcg acgagatgca gccgctggga ccgcgcgaac tccagggcgg
      901 gccgggagaa ccgcccgttg gtgaccagca cgacgacgtc accgtggtgc acgggccgcc
      961 cggtgccgtt gaggacatgc aggtccgggg tcccgaccgg agcccccgcc gaaccggcct
     1021 tgcggtgctt gcactggatg acccagcgcc gcccgtacgg gtcggtggcc ttgacgtcgg
     1081 ccccgtggtc cccggccccg ccgacctgga cggcgtcggt acagccgtcc cggaacatca
     1141 ggtcccgtac ggcatgctcg aacgcgcggt ggtgcagccg gtccaactgc tccacggcgt
     1201 accgcagcgt ccgggcccac tcctgctcac ggcggagccg gtcggcccgg tagcggaacc
     1261 agaggacacc gcccgccacg gcgaggagga ggacgagcag cacgacccac cggttcgcgg
     1321 cgacccagtc gagcagcctg ccgaccaccc agagcgcgaa gaggacgccg acggcgagga
     1381 cggcccagga ccgcccctgc tgcctgccgc gacggcggga gggcgtgcgc cgggagggag
     1441 agcgccggga gggggagcgt cgggccgggg tacggcgccg ggcgctcatc ggccgaagcc
     1501 cgcgaggcga acggctcccg ctcggcgcac cactccgttg ctggtcagca tggccgacac
     1561 ctctcccccg gtcccccgaa caccgacgtc cgtttctaac gcgcacggga ggacggcgcg
     1621 ccgggcttac cgggagttgg cgacaatgcg gcgctgagct ggccggatat cgcggcctcg
     1681 ggccggttgc ggaccgctga cggcgcgcga gccgaccgga gaaccgattc cgcaccgcct
     1741 gctccggccg cctggtcgcc tgctccgccg cctggccgcc tgctccggcc gccccctctc
     1801 ctcagccgga gcaggccgag cgtcccgcct cctgccaggc gcagaccggg cagagggtgg
     1861 cgcccttggt cgactcgggg tactcggtcg gctcacggca gagcacgcac tccgcgaacg
     1921 gctcggccgt gccggtgccc gtcggcgggg tggcggcggg tggtgtgcag tacgaggcgt
     1981 cgtcgtccat gtccacgagc gtacgtaccc ccgctgtcag ttggccgtcc gctccggcct
     2041 ggcctcgctc gggcggatga tgacgaagcc ctcgccctgg agcatcagct ggacggcctc
     2101 gcccgatccg ccgcggacca tcgagccgac gctctgggag cggtgcaggg tggtggtgag
     2161 gtgctggctc cagccgacga ccgcgtccgt gtcgacgtac accggctggg cggccgtcac
     2221 cgggatcacg atgggggtgc cctcgcacat caggccgatc ttgccgtaac cggagaagac
     2281 gctgttgaag aggccgccgc cgaccatccc ggcgcccttc accgtcctga tctcgtacgc
     2341 gagggtggcg tcgaaacaga ggacgttgcg gccgttgatg gtgagctggt cgccctggtc
     2401 catctccacg atgaaacagt tggcggcctc gtgcgcgaac cacgcctcgc cctgaccgcg
     2461 tacggccatg agcggcaggc cctcgccggt gaccgcgcgc ttgagcaggc cgccgatccc
     2521 ctggctcttg cgctcgaact ggaggttgcc gcggaaggcg atcatcgagc cctggcgggc
     2581 gtgcatctcc ccgttgacgg cgtacttgat cgacttggag ttctggaggg tcatgccggg
     2641 ggcggtggcc ggctgggcca ggtgttcgct ggaaaacaga tcgctcttca tgaccggcat
     2701 cctcacccgg accggctgat taagccaagg aggtgacggg ggccgcctgg cagactggga
     2761 cggtgagcag cgacgacacc gcccagagcc cctccggcga catctccccg agccccttcc
     2821 acgagggccc tgcgggttct gccgatcccg ccggttccgc ccgtcccgcc gacccggacg
     2881 cggccccgca gtacgtgctc ccgctcgtgc tgcacctgga gaagacggac ccgcccgccc
     2941 ggacggacgc cctgcgcacc gccgcccgcg cggtgctcac gatcctggcc gacgagcggt
     3001 ccacgggcga gggcgagtgg gcgaaggcca tgcgggactg ggaggacgcc cggatccgca
     3061 aggtggtgcg ccgggcgcgc ggcgcggagt ggcgcaaggc ggccgcgctg cccgggatca
     3121 cggtcacggg cgggagcgcg gaggtacggg tgtacccgcc gatcccgctc gacggctggc
     3181 ccaaggagct ggccaagctc caggtctcgg gcaccgacct ggacgacccc gagccacccg
     3241 ccgcccccga ccgggagggc ccggtgctct ggctcaaccc ggagctggag atgtccgcgg
     3301 gcaagaccat ggcccaggtc ggccatggcg cccagctcgc ctggtgggag ctgtccgaca
     3361 ccgaccgcaa ggcctggcgc gaggcgggct tcccgctctc cgtggccacc gcgcccccgg
     3421 accgctggcg cgagctgatc accagcggcc tgccggtggt ccgcgacgcg gggttcaccg
     3481 agatcgcacc ggggccgacg gtcgtggcgg agggcgggga gcgtttctgc ccgctgccgg
     3541 gggtacggac cgcccggcgg tgaacgcgcg gccgccgagc cgcttaccga ccgctttccg
     3601 gccgctttcc cggccgaccg ggtcaggcgg acctgtctgg cccgcctgac ctgcccggtc
     3661 cgcctgacct gcccggtccg cctgacctgc ccggtccgct tggccgcccg gtccacctgg
     3721 ccgccgagcc gcacaccgcc ttgccaggcc tgccaggcga ccaggccgac ctgccctgcc
     3781 tggcccgtct ggcccgtctg gccgcccagt ccgcccgggc tgcctgggct gcctgggccg
     3841 cctgggccgc ctgggccgcc tggccgcccg gccgcccggc cgcccggccg cccggccgcc
     3901 cggccgcaaa atagtccgcc cgcagttgag cattcccgtg cccgactacg tatctcccga
     3961 taccgcccca gcggcgctac cgccaagtag cctgcacggc acgccggttg gaggtcagcg
     4021 gtcgatcggt gatcggccgg tggccggtgg cctgtcggcg tgatcggcgc tgtcacgttc
     4081 tcgtcccggg agacaccttg ttgcgacgca tcaacggaac ggccctcatc atcgcggccc
     4141 tggtcgccac ggtcggcgcg ctcgccttcc ccgtctggtc ctacgcggac cgctcgggca
     4201 ccggcgaggc gaatctcaac gcctccagcg tcgccaccca gtggggcccg ctctccgcga
     4261 ccgaccggga cttcctggtc aaggtgcggc tcgccgggct gtgggagctg cccgccgggc
     4321 agcaggcgat cgagcgggcg cccagcgagg cgacgaaggc ggccggggac catctggttg
     4381 tcgggcacgc ggacctggac cggcgggccc gggacgtggc cgccaagctc ggggtggagc
     4441 tgcccaacca gccgaccgag cagcagcagg cgtggctccg ggagctgacg gcggccagcg
     4501 gggacgagta cgagcggaag ttcgccaaca tcctgcgggc cgcccacggc aaggtcttcg
     4561 ggctcatagg ccaggtccgg cacaccaccc gcaacaccct gatccggcag ctcgccagcg
     4621 acgccaacca gaccgtgctg gaccacatca ccatgctgga ggccaccggc ttcgtcgact
     4681 tcgacgccct ggcccgcgag gccgccggcg ggtccacggc cagcccctcg ggcccgcccg
     4741 caccccggga ggggcaggtg ccgctcgcgc cggtgcccgt gacgccctcc ggtgaccagt
     4801 cgttcacgtc ccgcccggtg ccgccgacca tgccgccgat gccctgacgt cgatgccgtg
     4861 acgtcaatgc agtgacgtcg atgccgtgac gtgacctgcg ggccggaacc tcaaatcctc
     4921 ctctgaacgt ccccctcttc gggttcaccg ccgttccgcg gggccatcac ccctggacag
     4981 agcggaggag ggggagacgg atgaggctgg gcatcgggat cggctggcgg ccggagatcg
     5041 ccgccgaggt ggaggcgctc acggggatcg actgggtcga ggcggtggcg gagaacctct
     5101 gcgccgacca cctccccgac tccctggtcc gcctgcggga gcggggcgtc accgtcgtac
     5161 cgcacggcgt ctcgctcggc ctgggcggtg ccgaccgccc ggaccccgag cggctcgcgg
     5221 ggctcgcggc ccgcgccgag ctgctcggta cgcccctggt gaccgagcac atcgcgttcg
     5281 tacgggccgg ggggccgctg accgcctccc cgcggctgga ggcgggccac ctgctgccgg
     5341 tgccgcgcac ccgggacgcg ctggacgtgc tctgcgagaa cgtgcgcatc gcccaggacg
     5401 cgctgcccgt gccgctggcc ctggagaaca tcgcggccct gatctcctgg cccgacgagg
     5461 agctgaccga ggggcagttc ctggcggagc tggtcgagcg gaccggggtg cggctgctga
     5521 tcgacgtggc caacctgcac accaaccacg tcaaccgggg cgaggacccg gcgaaggccc
     5581 tggacgagct gccggtggag gccatcgcgt acgtccacgt ggcgggcggg gtcgagaagg
     5641 acggcgtctg gcacgacacc cacgcccacc cggtgaccgc cccggtcctg gacgtcctgg
     5701 ccgagctgcg ctcccgcgtc gacccgccgg gcgtcctgct ggagcgcgac gacgccttcc
     5761 cgccgggcgc ggagctggcg gccgagctgg agacgatccg ggggacgctg cggtcggcgg
     5821 cccgcgccgg agcggtggcc cgggcggcag tgcccccgtc agcacccgca gccccggcag
     5881 cggctgcccc ggcggcagcg gtggccccgg cggcctcttc ggccctgccg accccttcgg
     5941 cctcttcgga cccttcggcc gatgcacgtg aggccggacc gctcggcgcc ccccgccccg
     6001 gagcccgcga ccgtaccgcc gtcgcccaga ccgcactgct ctccgcgctc gtcgccggga
     6061 ccccggcccc cgaggggttc gaccaccggc ggctgcgggt gcagagccgg gcgctggccg
     6121 ccaagcgggc cgatgtcgtc gccaaggtgg ctcccgagct gccggagatc ctcggtgacg
     6181 gctaccgtcc cgcgttcctc tcctacgcca ggtcccgccc gatgaacggc ggttaccggc
     6241 gcgacgccat ggagttcgtc gagcggatcc tggtctccgg cgggctcccc gaccccgtcg
     6301 cacagcgtcg gctgacgcac tggtgggagg accgctcggg gccccggccg ccgggccgga
     6361 ccacccgtct cgtccgggcg gcccgtgccg tcctcgtagg gaagtgacga ccgccatgaa
     6421 cacgttcgcc ctggtgctga ccctcgcggt cgtcctctcc accgcgctcc tcatcgggaa
     6481 ggccgtcgcc tcccggtccg gccgccgcac ccccgtcggc gccccctacc ccgtccgcga
     6541 cctctacgag gcggcgttcc tcaacggcgg tccggccagg gtcgcggaca ccgcgctcac
     6601 caccctgtac ggggacgggc gcgtgctcat cggcgggccc gggatcgtct ccgtacggca
     6661 cgagcaggcg cacgacccgg tggaacgcgc cgtgttccag gtgctggcga ccgcgcccag
     6721 cggtgccctg cacgcggtac gggaagccgt catgcgccac ccggcggtac aggagatcgg
     6781 ggaccggctg gcggagcgcg ggctgctggt gccgcccgcc gcgagccgtc ccgtacggcg
     6841 gtgggggctg atccaggggg tgagctgcat cgtcggcttc ccgctcgcca tcgccgccac
     6901 ggtcatccag tacgtgaacc acgactcgcc gttcgacatg ccgttcccgt tcgtcctgaa
     6961 ggtgctgccc gcgatcctgc tcggcgggat cagcgggctc gtcgtggcgg cggccgccgc
     7021 gggccggctc acccgggcgg gccgccgggc cgcccacgag ttccggaccg cccacgccta
     7081 ccaggtcacc ccgccgtacc tcgtcgccac ccaggggctg cgcgccctgc cggacccggt
     7141 gctccaggcc catctgatcg cggcggccgg ggtgaacgcc ccgtccgggc ggtcgcgtca
     7201 ccggtacggg acggcggggg tcgtgggcgg tacggcagcg gccgggggct tcgcggccac
     7261 cacggtctgg tgcgccgacg cgggcccggg gcacacgagt tgcggcgggt cgagcggcgg
     7321 cggctcgggc tccggatgcg gcggagggtc cgggtgcggc ggctccggat cgagctgtgg
     7381 tggcgggtcc agctgcggcg ggggctccag ttgcggaggc gggggcggct gcggcggcgg
     7441 ttcctgagcc gtcagcgctc gggggggggc ggttactgaa ccgtcagccc tcagggggtg
     7501 gttcctgagc cgtcggcctt cacgtagccg cgcctcccga accgtccgcc tcccggaccg
     7561 tccagccccc gcaccgtcag ccctcagaca gcaccctcac ccttcaggtg gcgcgaacgg
     7621 gtcgatccgc gcaacacgcc cgcccggtgc tggacttcac ccggccccac ccccaccatc
     7681 ccctctccgc atccgcagag aggccccgcc gtgagagcag tacccctgta cgcaacgatc
     7741 ggctcgctgg tcctgtcggc cctggtcggc accgcaccgg ccgccgcctc ggaccggacc
     7801 ggcggcggct ccgccgagga gcgcggtacg gcggtggcgg ccgcccgcgc ggcggcggcc
     7861 gggatctcct tcggggcctg cccggaggag gagatgttcc cggactccct ggagtgcggc
     7921 accgtacggg tccccctgga ctacgcccgc cccgacggcc cggccatccc gctcgccgtc
     7981 agccgcaccc gcgccaccgg tgaacccgcc gagcggcagg gctcgttcgt ctacaacccg
     8041 ggcgggcccg gcgcctccag catcaccttc ccgctgctgg gcgagatgtc cgagtggaag
     8101 gaggtggccg aggcctacga cctggtcggc tacgccccgc gcggcgtcgg cgcctccgcc
     8161 ccgctctcct gccaggaccc cgccgtcttc gcgcagagcc ccaccgactc ccccgcccac
     8221 cccaccaccg cgtacaagga gaagcggatc gcccaggcgc gggcgtacgt gaaagggtgc
     8281 gaggagagcg cgggcgaggc gctgcgccac tacacctcgc tcaacaacgc ccgtgacctg
     8341 gaggtgctgc gggccgccct cggggacgag cggctgacct tcatgggcgc ctcgtacggc
     8401 acgtacatcg gctccctgta cgcgaccctc ttccccgcac gcgtacgccg tatggtcttc
     8461 gattcggcgg tcgacccgga cccggagaag atctggtacc gctccaacct cgaccagtcg
     8521 ctcgccttcg agtcccgctg ggaggacttc cggcgctggg tcgcccggca cgacgacgtc
     8581 tacgggctcg gcgcctcccc cgaggccgta cagcgccatt acgacgacgt acgcaccgcg
     8641 ctcgcggccg aaccggcggg cgggaccgtg gggccgggac agctccacac ggggttcctc
     8701 caggccgtgt actacgacga ctactgggcg atgcgggcga cggccctctc cgagtacgtc
     8761 cgggggaacc cggagccgct ggtcacgcag ttgtcgccgc gcgccgtcac ggcgaaggac
     8821 gacgagaacg agcgggccgt ctacaccgcc gtcgagtgca acgacgcgcc gtggcccgag
     8881 gagtgggagg tctgggaccg cgaccacacg gacctcgccc gtaccgcgcc cttccagacc
     8941 tggcacaacg ccttcaccaa cctgccgtgc gccttctggc ccgcgccccg gcagcggccg
     9001 ctggacgtct ccaccgggtg gggcgagctg ccgccggtgc tgatcctggc ggcggagcgc
     9061 gacgcggcga ccccgtacaa gggcgcgctg gagcttcagc ggcggctgcg cggcgcggtg
     9121 ctcgtgacgg agcggggggc ggggacgcat ggtgtcggcg gattcgggaa cgcctgcgtg
     9181 gacgaccaca tgcggcggta tctgctgacg ggcgagacgc ccgccgggga cgcggagtgc
     9241 gccccgcgcg cggagccgaa ccccgtctcg ctggactagg gcctgtccgc ggtgtggaaa
     9301 cgggccctgt gggcgcactc tgggtgcgcc cacagggccc gaagccgaac ccggtgtcgc
     9361 tggaacggcg ggcggcacgg tcggtgccgg atgcccgtct tctgatcttc cgggttgggg
     9421 tggtgcggga actcgctggc tatgcgagcc cggccaccag gtctgcgatg tccttccggc
     9481 ggcccgtgta gaacgggatc tcctcgcgca cgtggagccg tgcctcggag gcgcgcaggt
     9541 ggcgcatcag gtcgacgatg cggtacagct cgtccgcctc gaaggcgagg atccactcgt
     9601 agtcgcccag ggagaaggag gcgacggtgt tggcgcggac gtccgggtag ccccgggcca
     9661 tcttgccgtg gtccgcgagc atgcggcggc ggtcctcgtc gggcagcagg taccagtcgt
     9721 aggaccggac gaaggggtag acgctgatgt agtcgcgcgg ggtctcgtcg gcgaggaagg
     9781 ccgggatgtg cgacttgttg aactcggcgg gccggtgcag ggccatgttc gaccagaccg
     9841 gttcgagagc gcgcccgagc ttggtgcggc ggaagaggtt gtaggcgtcc tggagctggt
     9901 ccgaggtctc ggcgtgccac cagatcatca ggtcggcgtc ggcgcggaga ccggagaggt
     9961 cgtaggtgcc gcgcacggtg acgtccttgg ccgcgagctg gtcgaacagc tcctggacct
    10021 cgtccgcgta tcccgtccgg tccagcggca gcacgtcgcg gagtttgaag accgaccaga
    10081 gggtgtagcg gatgacctcg ttgaggtcct tggccttctt tccggcgttc ggactcttgc
    10141 tgggtgtcac agtctcagga gcactcatac ggctattgtc ccgcctcgct cccggtgccc
    10201 cgaaccaggg tcggcgtggc gatgatctcg ctcgtgatct cccccgccga gtcgcccgtg
    10261 atccgccccg tcaggtcccg ggcgatctcg tcggcggccc gctgcgcgct cgcgatgcag
    10321 gcggggatgc cgaccccgtc gtaggccgcg ccgcagacgc ggagcgcggg cagtttcgcg
    10381 acctcgtcgc ggatgcgggc gacgcgggtg tggtggccga ccgggtactg cgggaggcca
    10441 ccgatccagc gggtgacctc ggtgtcgacg gggcgggcgg cgagcccggt ggcctcgcgg
    10501 aggtcccgca gggagacggc gacgagttcg ccgtcctcgc ggtgcaggtg gtcctcctcg
    10561 ccgtagcggc cgacggaggt gcggagcacg aagaggtcgg gggcggcgtc cgcgacccac
    10621 tgccacttgt tgctggagaa ggtggaggcc ttgatggtgc ggccgtcgac cggcggtacg
    10681 agaaagccgg agcggccgcg cagggccgtg agcccctcga tgtcggcgcg ccggaaggcc
    10741 atggtgacca gggccatcga ggcgtactcg acgccggtca gctcggtgga ggcggccggg
    10801 gactcggcgg ccagcagggc ggaggcggac caggcggggg tggcgaggac gacgccgtcg
    10861 gcggtgatca ccccggtgtc ggtgcggacc gtccagcccg tggccgtacg ggtcaggccg
    10921 aggaccgggg tctcggtcag gatctcgccg cccccggccc gtacggcatc gcccacggcc
    10981 ccgggcaggg tgccgatgcc gcccttcagg ccctggaaga cggggccggt ctgctgccgg
    11041 gccgcggcgc gctcctggat gcggcggacg gcgtccagca gcgggccacc ctccttcacc
    11101 tcttcgaaga gctgcggtac ggcggcgcgc agcgagatcc ggtaggcgtc gcccgcgtag
    11161 accccgccga gaaggggctc caccagccgg tcgacgacct cgcggccgag ccggtcggcc
    11221 acgtacgcgc cgacggccac gtcgtccccg acgggcgtcg gggtcaggtc gcgctcctcg
    11281 gcgatccggg ccagcccctc gggggagagc accccctcca gggcggccgg gtcgccgggg
    11341 acgcccatga cgtggccctt gggcatgggg cgcagcgcgt cgcgggtcca gagggaggcg
    11401 gtggcgg
//
