LOCUS       NZ_CP012382            10216 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Streptomyces ambofaciens ATCC 23877 chromosome, complete genome.
ACCESSION   NZ_CP012382
VERSION     NZ_CP012382.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces ambofaciens ATCC 23877
  ORGANISM  Streptomyces ambofaciens ATCC 23877
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8303940)
  AUTHORS   Thibessard,A., Haas,D., Gerbaud,C., Aigle,B., Lautru,S.,
            Pernodet,J.-L. and Leblond,P.
  TITLE     Complete genome sequence of Streptomyces ambofaciens ATCC 23877, the
            spiramycin producer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8303940)
  AUTHORS   Thibessard,A., Haas,D., Gerbaud,C., Aigle,B., Lautru,S.,
            Pernodet,J.-L. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Faculte des Sciences et Technologies,
            Universite de Lorraine, Boulevard des Aiguillettes, Vandoeuvre les
            Nancy F-54506, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC workbench v. 7.0.3
            Genome Coverage                   :: 114
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001267885.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 02:57:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,510
            CDSs (total)                      :: 7,418
            Genes (coding)                    :: 7,242
            CDSs (with protein)               :: 7,242
            Genes (RNA)                       :: 92
            rRNAs                             :: 5, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 74 of 176
            Pseudo Genes (incomplete)         :: 131 of 176
            Pseudo Genes (internal stop)      :: 12 of 176
            Pseudo Genes (multiple problems)  :: 39 of 176
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:42:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7314193
            Orig. end                         :: 7324409
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012382.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="24"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1742..3967)
                     /locus_tag="SAM23877_RS32160"
                     /old_locus_tag="SAM23877_6773"
     CDS             complement(1742..3967)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="SAM23877_RS32160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_6773"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_053140871.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.1e-38, bitscore:
                     130.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAQETRGRRRAAPPKRKVPRSGRAKTVRTPTVLQMEAVECGAA
                     SLAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARGYGFTAKGMQMDLAALAEVT
                     APAILFWEFNHYVVYDGVGRRFGRRGVFINDPGKGRRFVTLEDFDTSFTGVVLTMEPGE
                     DFTRGGRRPGVLGAMPARLRGTAGTLPAAVLASLLLVAVGAAVPALSRTYIDTFLIGGQ
                     TSLLGVLFTSMAACVLLTVVLTWLQQANLLHGRIISSTLSSARFLRHLLRLPVTFFSQR
                     SPADLVQRLQSNDAVAETLARDLAAAGVDAIVVVLYAVLLYTYDPQLTFVGIGVALLNI
                     LAMRIVIRLRATRTAKLRADTARLTNTAYTGLQLIETMKATGGEDGYFRKWAGQHATTL
                     EEQQRLGVPSAWLGVVAPTLATLNSALILWIGGMRAVEGHISVGLLVAFQALVVRFTAP
                     LTRLNGVAGRIQDFAADVARLKDVENFRADPLYDRPGGIDSTRRLHGHVELQNISFGYN
                     PLDKPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYTPWDGVIRIDGQRIDDIP
                     RGALASSVSFVDQDVFLFEGSVRDNVALWDPSVPDEAVVEALRDAALYDVVTRRPGGIH
                     SRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETEQVVMDNLRRRGCAC
                     VVIAHRLSTVRDSDEIVVLEHGTVVERGRHEELVARGGAYAALVRER"
     gene            complement(3964..4770)
                     /locus_tag="SAM23877_RS32165"
                     /old_locus_tag="SAM23877_6774"
     CDS             complement(3964..4770)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="SAM23877_RS32165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_6774"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_053140876.1"
                     /transl_table=11
                     /translation="MQFRQQALAKLQSPEELDLPVRFARPQGWAVLSVTLVAMAAACVW
                     AVGGSVTSTVGAPAVLTHGQGSYLLQSPVSGQVTAVLARQGERLPADSPVLKVRTAEGE
                     TVVRTVDAGRVSALAATVGQIIQTGANVASVEKVAHADDPLYATVYVPAEKAAAIPAHA
                     SVDLTVQSVPAQQYGVLHGKVQSVDRSAQSAQTISAFLGDGQLGEQFTKEGRPVAVTVR
                     LTTSKSTRSGYEWSSADGPPFGLTSMTMATGSIRLADQRPLDWLLP"
     gene            complement(5001..5216)
                     /locus_tag="SAM23877_RS32170"
                     /old_locus_tag="SAM23877_6775"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="SAM23877_RS32170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_6775"
                     /product="hypothetical protein"
                     /protein_id="WP_053140878.1"
                     /sec_met_domain="strepbact (E-value: 3.1e-37, bitscore:
                     125.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEISDADLDNVSGGLSVNAVGTVTGLVDGIAPVSGL
                     LNTAIGTVEGVTGLNTAPVTNLVAGL"
     gene            5783..7270
                     /locus_tag="SAM23877_RS32175"
                     /old_locus_tag="SAM23877_6776"
     CDS             5783..7270
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134775.1"
                     /locus_tag="SAM23877_RS32175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_6776"
                     /product="S1 family peptidase"
                     /protein_id="WP_053140881.1"
                     /transl_table=11
                     /translation="MSHKRIPKRRAAMAAGGVVALGAAAILLPNANASQDGGSAGGTAA
                     PKTLKAADASDLASQLAGLLGDAFAGSYYDAGEQQLVVNVVPGDNNQVIVQAKKAGAKV
                     REVDNSLGELRGGARTLKAEATVPGTAWAIDPRTNKILVTADSTVTGAAWDRVESTVEG
                     LGSGMATIRKSPGTFKTFVSGGDAIFGGGARCSLGFNVTAGDGAPAFLTAGHCGVADDQ
                     WSDAQGGQPIATVDQAVFPGEGDFALVRYDDPATEAPSEVNLGDQTVQISQAAEATVGQ
                     QVFRMGSTTGLADGQVLGLDATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTQDGLAIGL
                     TSGGSGDCAVGGETFFQPVTTALEAVGATLGGDASGGAGDQAGGEEAGGEEAGDGQAGA
                     GGDAGAGEDAGAGAGDVGDGTGVGGEEAGAGDQAGGEEAGGGDAVGGGEEAGGEEAGDG
                     QAGAGGDAGAGEDAGAGAGDVGDGTGVGGEDGSGLTG"
     gene            complement(7699..9840)
                     /locus_tag="SAM23877_RS32180"
                     /old_locus_tag="SAM23877_6777"
     CDS             complement(7699..9840)
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994900.1"
                     /locus_tag="SAM23877_RS32180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_6777"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_079030846.1"
                     /transl_table=11
                     /translation="MADDQPSAPAPDTATVSGGHPVLSLALAAMMEDVHAHSGAVYLLG
                     ADPPVLEMAVMAGLPRAFAAPWERVTLHAPVPVADAVRERRTVWVGDEEEMARRYPRVA
                     VVLPYPFALVALPVATESTAYGAVFLTWPGARATELSERELRRLTAACERLAVRLERAA
                     AASVPVHPEPDLLAAPVVGGAPGTLGSLEAVRMVGRLPYGLCSLDLHGRVGFANPAAAE
                     LMGVPVAELIGTHLWTSVPWLNDPVYEDRYRAALFSQHATSFVALRPPGDWLSFRLYPT
                     RTGLSVRISRARGVSRPGSAGAVPDGAAPPRLVTISQVVSLTGALSEAVGVRDVVQLIA
                     DEIAPTVGSQGLVVLGSRAGRLHVLGHRGYPDPRAVERFDGTPLTEAIPGAQVLRTGVP
                     AFFESWEQLERLYPARRGTHDGFAAWAFLPLIASGRPVGTCVLAYSRPHSFTTEERAIL
                     TSLAGLVAQALDRALVYDAKYRLAHGLQDALLPHSLPLLPGVEAAARYLPATRGMDIGG
                     DFYDLVPAGGAPVAVIGDVQGHNVTAAGLMGQVRTAVRAYTAVGQAPEEVVRSTNRLLI
                     DLGTELFVSCLYLRLDAARGRATLARAGHPPPLLRRPDGRVEVLDVAGGPLLGIDPSAA
                     YPCTEIDLAPGSLLALYTDGLVESPGADFEDALADLGHRLADAGDLPLDALADRLVRDE
                     AGAEERPDDIALMLLRAKG"
     misc_feature    9854..9869
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.73"
                     /tool="antismash"
ORIGIN
        1 cttgggcagc ccgcgcaggg tctggaacgc ctggttgttg agcttgttgg tgagctggac
       61 cagccgccgc tgccagcgca cctgccacag cccgaggccg aggaagacgg cggcgacgac
      121 caccagcatg ccgatcgcgg cgagcgccat cggcacgctg taccagagca gcagccccag
      181 gttcatcgcc ccgacggtca cggactgcgc caccgtcgga ccgacccccg ccagcagcct
      241 gcggatggcg ctgatgccca tggcggcgct cgccagctca cccgtggagc gctcggtgaa
      301 gaacttcgtc ggcagccgca gcagccggtc ccacacggcc ggctggaggg tcgcctcgat
      361 gcggccctcg agacgcagga tggtgaggtt ctgcaggagc atgaaggccg ccgcgacgac
      421 gctgctcagc atcaccgcga gacagacctg cacgatcagc ccggtctgcg ccttcggcac
      481 gaactcgccg agcaccttgc cggtggcgat cggcaccagc gcgccgatcg ccaccgtcac
      541 cagcccgctg agcagcaggc cggtcagatc accgcgggtg ccctgcaggc agaagcgcag
      601 cagccgcagc gggctgagcc ggcgttcggg cagcggccgg tagaacatca ccgcgcgggg
      661 ctcgaactcc tcggcgttcg ccttctcgac cggcgtctcc cggccggtcg ccggatggac
      721 ggccacgtag ccgccccgcc gccacagcag cgccaccggc gcgccggaca gcgcccggtg
      781 gccgaccagc ggcccgacgt cgtcccgcca ccaccggtcc tccagccgga cggtccgggt
      841 gcgcaggcgc gaggcgacgg cgacccgctc caccgggtcg atccggtcgc tccccgtgtc
      901 gccgcgcggg ggctcggcga gcgggatgcc ggcggcacgg gcgaccagct tgcaggccgc
      961 gtaggtggcg tcggcgtcgg cggccgtggc gcgcggcccc gagggcttgc cgatggaggc
     1021 cagcaacgtc cggtcggcct gggcgcgcac cgcctcaccg gccttgatgc cggccgccgt
     1081 gcgggtctcg tgggtgcgct ccagctgttc gatccagcgg tccagcgtgg tcagcagccg
     1141 gtactgctgg tcgaccatgc tctgccacag cgccgggtcc atcagcaggt cggcggcggc
     1201 ctcgtcgccg tacaccgcgc cgtactggac gctgcccggg gggacctgca tccagaacac
     1261 gtcgtcgtcc gtgggcgccg cggcccgttc gtcggccatc ggcgcctgga agaggacggt
     1321 caggccgcgg cccaccccga gggcgagggc gtactccagc gggctcgtcg tcggtggcac
     1381 gtactggggg ttgccgtacg cgtcgtacga ccacgtctgc gtgttcgccg gctggtacag
     1441 ctcgcgcagc ccgatgcggt gcaccacgca gtcccgcagc ggacgcgcga ccagcgtgtg
     1501 ctggggcccg gagaccggcc cgagcagcac cgagcccgcc tccaggcggc ccaggtggtg
     1561 ccagtgcccc tgctgcccgg cgtccaccgc gaacaggtcc accgcgccgg acgccaccag
     1621 ccacagcacc tgcgggccct ccaggtcgag gcggccgaac ccggcgcagt cgacgcgcgt
     1681 gcccagcgag ccgagcgccc cgaggacgac gtcgccctgt ccttcgtgca ccgctgtcat
     1741 ctcaccgctc cctgaccagc gccgcgtacg cgccaccgcg cgcgaccagc tcctcgtgcc
     1801 gtccgcgctc cacgaccgtg ccgtgctcca ggacgacgat ctcgtcgctg tcccgcacgg
     1861 tgctcagccg gtgcgcgatc accacgcagg cgcagccgcg ccggcgcagg ttgtccatga
     1921 cgacctgctc ggtctccgcg tcgagcgcgc tggtcacctc gtcgaggacg aggatgctgg
     1981 gccggcgcac cagggcccgt gcgatctcca gacgctggcg ctgcccgccg gagaagttcc
     2041 ggccgtcctg ctcgaccctg ctgtggatgc cgcccggacg gcgcgtcacc acgtcgtaca
     2101 gggcggcgtc ccgaagcgcc tccaccacgg cctcgtccgg gaccgacggg tcccacagcg
     2161 ccacgttgtc gcggacggac ccctcgaaga ggaacacgtc ctggtcgacg aaggagacgg
     2221 aggacgccag ggcgccgcgc gggatgtcgt cgatgcgctg cccgtcgatg cggatcacgc
     2281 cgtcccacgg ggtgtacagc cctgagatca gccgggagac cgtcgacttg ccgctgcccg
     2341 agccgccgac cagggccacc tgctgccccg gtccgacggt caggtcgaag cccgtgagca
     2401 ggggtttgtc cagcgggttg taaccgaagg agatgttctg cagctccaca tggccgtgca
     2461 gccggcgggt cgagtcgatc ccgccgggac ggtcgtagag cgggtccgcc cggaagttct
     2521 ccacgtcctt cagccgggcc acgtcggccg cgaagtcctg gatgcggccc gcgacgccgt
     2581 tcagccgggt cagcggggcg gtgaagcgga ccaccagcgc ctggaaggcg accagcagcc
     2641 ccaccgagat gtgcccctcc accgcgcgca tgccgccgat ccacaggatc agcgcgctgt
     2701 tgagcgtcgc gagcgtcgga gccaccacgc ccagccaggc gctcggcacg ccgagccgtt
     2761 gctgttcctc cagcgtggtg gcgtgctgcc cggcccactt gcggaagtag ccgtcctcac
     2821 cgccggtcgc cttcatcgtc tcgatcagct ggagaccggt gtacgcggtg ttggtcagcc
     2881 gcgccgtgtc cgcccgcagc ttcgccgtac gggtcgcgcg cagccggatg acgatccgca
     2941 tggcgaggat gttgagcagg gccacgccga tgcccacgaa ggtgagctgc gggtcgtacg
     3001 tgtacagcag tacggcgtac agcacgacga cgatcgcgtc gacgcccgcg gcggcgaggt
     3061 cgcgggccag cgtctcggcc accgcgtcgt tggactggag gcgctgcacc aggtcggccg
     3121 ggctgcgctg ggagaagaac gtcaccggca gccgcagcag gtggcgcagg aagcgggcgc
     3181 tggagagggt ggaggagatg atgcggccgt gcagcaggtt ggcctgctgg agccaggtca
     3241 gcaccaccgt gaggagcacg caggcggcca tcgacgtgaa gagcacgccc agcagcgagg
     3301 tctgcccgcc gatcaggaac gtgtcgatgt aggtgcggct gagcgcgggc accgcggcgc
     3361 ccaccgccac cagcagcagg ctcgccagca ccgcggcggg cagggtgccc gcggtgccgc
     3421 gcagccgggc cggcatcgcg ccgagcacgc cgggcctgcg gccgccccgg gtgaagtcct
     3481 cgccgggttc catggtcagg acgacgccgg tgaagctcgt gtcgaagtcc tccagggtca
     3541 cgaaacggcg gcccttgccc gggtcgttga tgaacacccc gcggcggccg aaccgccggc
     3601 ccacgccgtc gtagacgacg tagtggttga actcccagaa caggatggcc ggcgccgtca
     3661 cctcggcgag ggcggccagg tccatctgca tgcccttggc ggtgaaaccg taaccgcgcg
     3721 ccgccttcag caggttgctc gctcgcgagc cgtcgcggga gacgccgcag gcgatgcgca
     3781 gctcctccag cggcacgtgc cggccgtagt ggccgagcac catggccagg gaggcggcgc
     3841 cgcactccac cgcctccatc tggagcacgg tcggggtgcg gacggtcttc gcccggccgc
     3901 tcctgggcac cttgcgcttc ggcggggcgg cgcggcgtct gccccgggtc tcctgtgcgg
     3961 tggtcacggc agcagccaat cgaggggacg ctggtcggcc agccggatgg aacccgtggc
     4021 catggtcatg gaggtcaggc cgaagggcgg tccgtccgcg gacgaccact cgtagccgct
     4081 ccgcgtggac ttggaggtgg tcagccggac cgtcaccgcc accggcctgc cctccttcgt
     4141 gaactgctcg ccgagctggc cgtcgccgag gaaggcggag atggtctgcg ccgactgggc
     4201 cgagcggtcc acggactgca ccttcccgtg cagcacgccg tactgctggg cgggcaccga
     4261 ctggacggtt aggtcgacgg aggcgtgcgc ggggatggcg gcggccttct cggcggggac
     4321 gtagacggtg gcgtagagcg gatcgtcggc gtgggcgacc ttctcgaccg aggcgacgtt
     4381 cgcaccggtc tggatgatct gacccacggt ggcggcgagc gcgctgaccc ggccggcgtc
     4441 gacggtgcgg accacggtct cgccctcggc ggtgcggacc ttcagcacgg gggagtcggc
     4501 gggcaaccgc tctccctggc gcgcgaggac cgcggtgacc tggccggaca ccgggctctg
     4561 gaggaggtaa ctgccctgtc cgtgggtgag gacggcgggt gcgccgacgg tggaggtcac
     4621 cgagccgccc accgcccata cgcacgcggc ggccatcgcg acgagggtca cggaaagcac
     4681 cgcccagccc tgcgggcggg cgaaacgcac cgggagatcg agctcctccg gtgactggag
     4741 ttttgcgagg gcctgttggc ggaactgcac gtcggacgta tcccagagtt ttccggcgaa
     4801 gccacggaag cgaccgtgga cgcgaagcca cggacgagac cgcggacgga gccacggaag
     4861 gaaccgcgga cggagccacg gaaggaaccg cggacggagc cacggaggaa accgtgggcg
     4921 gcgccacgga agccgtggag gaacggccga aaagccccgg agcctgggtg gctccgggac
     4981 tcagcggcac agaggcgcga tcagagaccg gcgaccaggt tggtgaccgg ggccgtgttc
     5041 aggccggtga cgccctcgac ggtgccgatg gcggtgttga gcaggcccga gacgggggcg
     5101 atgccgtcca ccaggccggt gacggtgccg acggcgttca cggacagacc gccggagacg
     5161 ttgtcgaggt cggcgtcgga gatctcgacg gtctcaacct ggggggtgga gttcatgggg
     5221 gaacttccct tcgtatagcc atttcacaag gggggagcgg ccccctctgg ggacagatga
     5281 cggccgcgac cgcgggcgcc gggcgccctt ttccggagcc ctccgcggcc cccgcggtgc
     5341 gatggatcaa agcacgcggc cggtccgggc ttccaatcaa tgaacccctc ccaccagggc
     5401 acttgcgacg cccgggcaac caaccgtgca caatcgcgca cgccttcacg gcgacttctt
     5461 cacaaaggcc gcgacatcgg cccgtgcgag cccttgcccg gggcggcgca tccggcaccg
     5521 cggcccggaa ggcgtacggg gtgccgtgcg gttgtgcaga tcctccagtg ggggtgaccg
     5581 ggttgggcat tcgatgtgca gattcgctga agccaggatt ccgctccctg atcggtacgg
     5641 accgttgtcg atcgatcgct gagcgtgtcg atccggtgcg tttgaacggc ccgtcgggcc
     5701 cgtgcgtacc aacagccatc caggcgcccc ccgacagctg ccgtaccggc ggcacggacc
     5761 ccgtcccagg aggtcgagaa ggttgagtca caagcgaatt ccgaagcgca gggccgcgat
     5821 ggcggcaggc ggcgtggtgg cgctcggcgc ggccgccatc ctgctgccga acgccaacgc
     5881 ctcgcaggac ggcgggtcgg ccggcggcac ggcggcgccg aagaccctca aggcggcgga
     5941 cgcgtcggat ctcgcctcgc agctcgcggg cctgctcggc gacgccttcg cgggctccta
     6001 ctacgacgcc ggcgaacagc agctcgtcgt caacgtcgta cccggcgaca acaaccaggt
     6061 gatcgtgcag gcgaagaagg cgggcgcgaa ggtgcgcgag gtcgacaaca gcctgggcga
     6121 actgcgcggc ggcgcgcgga ccctgaaggc ggaggcgacc gtcccgggca ccgcgtgggc
     6181 gatcgacccg cgcaccaaca agatcctggt caccgcggac agcaccgtca ccggcgccgc
     6241 gtgggaccgg gtggagtcca ccgtcgaagg cctcggctcc ggcatggcga ccatccggaa
     6301 gtcccccggc accttcaaga ccttcgtctc gggcggggac gccatcttcg gcggcggcgc
     6361 tcgctgctcc ctcggtttca acgtcaccgc gggcgacggc gcgcccgcct tcctgacggc
     6421 cggtcactgc ggcgtggcgg acgaccagtg gtccgacgcg cagggcgggc agccgatcgc
     6481 caccgtcgac caggcggtct tccccggcga gggcgacttc gcgctcgtgc ggtacgacga
     6541 cccggcgacc gaggcgccga gcgaggtgaa cctcggcgac cagacggtcc agatcagcca
     6601 ggcggcggag gcgacggtcg gtcagcaggt cttccgcatg ggcagtacga ccggactggc
     6661 cgacggtcag gtcctcggcc tggacgccac cgtcaactac ccggaaggca cggtcaccgg
     6721 cctcatacag accgacgtgt gcgccgaacc gggcgacagc ggcggctccc tgttcaccca
     6781 ggacggcctc gcgatcgggc tgacctccgg cggcagcggc gactgcgcgg tgggcggcga
     6841 gaccttcttc cagccggtca ccaccgccct ggaggcggtc ggcgccaccc tcggcggcga
     6901 cgcgtccggt ggcgcgggcg accaggcggg cggcgaggag gccggcggcg aggaggccgg
     6961 tgacggccag gccggtgccg gtggcgacgc cggtgcgggt gaggacgccg gtgccggtgc
     7021 gggcgatgtc ggtgacggca cgggcgtcgg cggcgaggag gccggcgcgg gcgaccaggc
     7081 cggtggggaa gaggccgggg gcggtgacgc cgtcggcggt ggcgaggagg ccggtggcga
     7141 ggaggccggt gacggccagg ccggtgccgg tggcgacgcc ggtgcgggtg aggacgccgg
     7201 tgccggtgcg ggcgatgtcg gtgacggcac gggcgtcggc ggcgaggacg gctccgggct
     7261 gacgggctga cgggctgacg cggtcgcccc gcgcccgcgg cgcgcctcac gaaccccggc
     7321 cggctcccga ggggccggac ggcggtgggc ggtccgctcc tccggcggga ggggcggacc
     7381 gccgcacggc ggagccgcag ccgagcggcc gggcgccgca ggcgtggggg agcggggcga
     7441 cgggtctgta ccggctctgc ggccaccgaa ggagaacccc cgcgtcccga ccggagcacc
     7501 cgcgtcccga ccggagcgcc cgcgtcccgg cccgagcacc cgcgtcccga ccggagcacc
     7561 cgcgtcccgg cccgagcccc cgcctcccga ccgcagcacc cgcgtcccga ccggagcacc
     7621 cgcgtcccgg cccgagcgcc cgcctcccgg cccgagcacg catctcggcc gaggttccgg
     7681 cgccccgcgg gccccgcctc agcccttcgc tcggagcagc atcagggcga tgtcgtcggg
     7741 gcgttcctcc gcaccggcct cgtcgcgcac caggcggtcg gcgagcgcgt ccagcggcag
     7801 gtcgccggcg tcggccagcc ggtgccccag gtcggccagg gcgtcctcga agtcggcgcc
     7861 gggggactcc accaggccgt ccgtgtacag ggccagcagg gagccgggag ccaggtcgat
     7921 ctccgtgcac ggatacgcgg ccgaggggtc gatgcccagc aggggcccgc cggcgacgtc
     7981 cagcacctcc acccgcccgt ccgggcggcg cagcagcggc ggcggatgtc ccgcgcgggc
     8041 cagtgtcgcc cgcccgcggg ccgcgtcgag ccgcaggtac agacagctga cgaacagctc
     8101 ggtgcccagg tcgatcagca gccggttggt gctgcgcacg acctcctcgg gcgcctggcc
     8161 gacggccgtg tacgcgcgga ccgcggtacg cacctgcccc atcagcccgg ccgccgtcac
     8221 gttgtgcccc tggacgtccc cgatcaccgc caccggcgcg cccccggccg gcaccaggtc
     8281 gtagaagtcg cccccgatgt ccatgccccg ggtggccggc agataccggg ccgcggcctc
     8341 gacgccgggt aggaggggca gcgagtgcgg cagcagggcg tcctgcaacc cgtgcgccag
     8401 ccggtacttg gcgtcgtaga cgagggcgcg gtccagggcc tgcgcgacca gcccggccag
     8461 gctggtcagg atcgcccgct cctccgtggt gaaggagtgc ggtcgtgagt aggcgaggac
     8521 gcaggtgccc accggccgcc cggaggcgat cagcggcagg aacgcccagg ccgcgaaccc
     8581 gtcgtgggtg ccccgtcgcg ccgggtacag ccgctccagc tgctcccagg actcgaagaa
     8641 ggcgggcacc cccgtccgca ggacctgcgc gcccgggatc gcctcggtga gcggagtccc
     8701 gtcgaaccgc tccacggcgc gcgggtccgg atagccgcgg tgtcccagga cgtgcagccg
     8761 cccggcccgt gagccgagga cgaccaggcc ctgactgccc acggtcggcg cgatctcgtc
     8821 ggcgatcagc tgcaccacgt cccgcacacc caccgcctcg gacagcgcgc cggtgaggct
     8881 gaccacctgg gagatggtca cgagccgggg cggggcggcc ccgtccggca ccgccccggc
     8941 cgagcccggc cgcgacaccc cccgggcccg gctgatgcgc acgctgagcc cggtgcgggt
     9001 cgggtacagc cggaacgaga gccagtcgcc gggcggacgc agcgccacga aggaggtggc
     9061 gtgctggctg aacagcgcgg cccggtaccg gtcctcgtac accgggtcgt tgagccacgg
     9121 caccgacgtc cacaggtggg tgccgatcag ctccgcgacc ggcacgccca tgagctcggc
     9181 cgcggccggg ttggcgaacc ccacccggcc gtgcaggtcc agggagcaca gcccgtacgg
     9241 cagccgcccc accatccgca cggcctccag cgacccgagg gtgccgggcg cgcccccgac
     9301 caccggggcg gccagcagat cgggctccgg gtgtacggga acactcgccg cggcggcccg
     9361 ctccagccgc accgcgagcc gctcgcaggc cgccgtcagc cgccggagct cccgttcgga
     9421 cagctccgtg gcacgcgcac cgggccaggt caggaacacc gcgccgtacg ccgtggactc
     9481 ggtcgcgacg ggcagcgcga ccagggcgaa cgggtagggc aggaccacgg cgacccgggg
     9541 atagcgccgc gccatctcct cctcgtcgcc gacccagacc gtccgccgct cacgcaccgc
     9601 gtccgccacc ggcaccggcg cgtgcagcgt cacccgctcc cagggcgccg cgaaggccct
     9661 cggcaggccg gccatcaccg ccatctccag caccggcgga tcggcgccga ggaggtagac
     9721 cgccccggag tgcgcgtgga cgtcctccat catcgcggcc agcgccaggg agagcaccgg
     9781 atggccaccc gacacggttg cggtgtcggg tgccggtgcg gagggctgat cgtcggccac
     9841 gccgaccacc tcctcgcacg cctgtgcacg ggaacccccg ggttcaaatc tcctccccga
     9901 aggcccgccg cgcacggcga gcaccgcccc ggcaggtccg cgagcgggca ggggagtcac
     9961 ctcgcgcgtc caggcacggg cagaggaccg ctccgcgcat ccgggcacgc ggagccgccc
    10021 tggcacgcgc ccgactgcgg gcacggtgtc gccccggcag gttcgggcgc gggcagggag
    10081 ctgccccgac acctccgccc cccatgcgcg gcgccgcccc gacacctccc ctcacgcgcg
    10141 gcgccgcccc gtcacctccc ctcacgtgcg gcgccgcccc gtcacctccc ctcacgtgcg
    10201 gcgccgcccc gtaacc
//
