LOCUS       NZ_CP012382            10363 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Streptomyces ambofaciens ATCC 23877 chromosome, complete genome.
ACCESSION   NZ_CP012382
VERSION     NZ_CP012382.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces ambofaciens ATCC 23877
  ORGANISM  Streptomyces ambofaciens ATCC 23877
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8303940)
  AUTHORS   Thibessard,A., Haas,D., Gerbaud,C., Aigle,B., Lautru,S.,
            Pernodet,J.-L. and Leblond,P.
  TITLE     Complete genome sequence of Streptomyces ambofaciens ATCC 23877, the
            spiramycin producer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8303940)
  AUTHORS   Thibessard,A., Haas,D., Gerbaud,C., Aigle,B., Lautru,S.,
            Pernodet,J.-L. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Faculte des Sciences et Technologies,
            Universite de Lorraine, Boulevard des Aiguillettes, Vandoeuvre les
            Nancy F-54506, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC workbench v. 7.0.3
            Genome Coverage                   :: 114
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001267885.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 02:57:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,510
            CDSs (total)                      :: 7,418
            Genes (coding)                    :: 7,242
            CDSs (with protein)               :: 7,242
            Genes (RNA)                       :: 92
            rRNAs                             :: 5, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 74 of 176
            Pseudo Genes (incomplete)         :: 131 of 176
            Pseudo Genes (internal stop)      :: 12 of 176
            Pseudo Genes (multiple problems)  :: 39 of 176
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:42:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3468492
            Orig. end                         :: 3478855
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012382.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10363
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="10"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10363
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10363
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5363](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5363)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(56..65)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    316..330
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.14"
                     /tool="antismash"
     misc_feature    complement(614..629)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.39"
                     /tool="antismash"
     gene            923..2224
                     /locus_tag="SAM23877_RS15345"
                     /old_locus_tag="SAM23877_3257"
     CDS             923..2224
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975609.1"
                     /locus_tag="SAM23877_RS15345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_3257"
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="WP_053142514.1"
                     /transl_table=11
                     /translation="MQPQPQPQPPSRRIRPRRLAAIATVTVVTFAVSTSAGTGRLAPGT
                     TAAGPGGPAALSRSSAHGPCMITGGPEIQMSEGVPTSAGYARSTGTVRALTLMIDFSDA
                     PGEGEALDRYAEFFPQTEEWFRTSSYGRLDYRPETPLRDWLRMPKSFAEYGIERGAPFE
                     PGYREMVQDLVAEADAKVDFQEYDLLNVLVTPNAGPSALDTVLSVTFAGNPEAPVADGV
                     PVSNASFVYSRQDDGSGSYDETGYRVLPHENGHVFGLPDLYTQEGGGAVGHWDIMSEDW
                     GANNDVLGWHKWKLGWLDPAQVHCASAPGTREYTLTPLARKGGDKVVVVPLDGRSGYAV
                     ELRTREGNDETVCRPGVLIYKVDADVDTGMGPVTVFDSRRDSGGCTRSPNVHAELSDAA
                     FVPGETFEDPRRGITIAVSGADPNGDHKVRVTRG"
     gene            2331..3944
                     /locus_tag="SAM23877_RS15350"
                     /old_locus_tag="SAM23877_3258"
     CDS             2331..3944
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007388760.1"
                     /locus_tag="SAM23877_RS15350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_3258"
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_053132656.1"
                     /transl_table=11
                     /translation="MPAHTTNDTPRDEGVAAPAEAVAPLLRGIAVLRRLTEADGGTLSL
                     SALEKATGLARSTVDRVTATLARMGYVRLDGRDVTLAPRLMELGNAYLAALRLPALLSA
                     RADALADALDESVSLAVGDRDGIRFIHQATRRRAMSLSFRIGDLLPAERTAPGPLFATE
                     WTDADWRRWHDRRATDPRDTTFPAVPPREHTTPDEDFEHRTARAAADGWALDDQLIEPG
                     LVAVSVPVRDPGSGRVACVASVVSHTSRHTAGDLRAALLPRLRTAVAAMEDDLRTAPPP
                     EPGAPPAGLAVWTGASKQELGREFVESLARGLTVLTAFGESRPAPTLTEIAKATGLARA
                     TVRRALITFEHAGLVAPAPGRAFTLTPRVLSLGFPPLSRASLPEIAQPHLTALADRVRE
                     PAALAVLADSGEEIQYTARASTSHVLSAHVTVGARRPAYATAPGRVLLAGLPADPLPEV
                     RAQGHALVDEELEAGLRSLAVPVRDRTGRVVAAVNVATHAARRSAVSCVADVLPELRRT
                     ADLVEADLRVAGRFRTVPTT"
     gene            complement(4051..4989)
                     /locus_tag="SAM23877_RS15355"
                     /old_locus_tag="SAM23877_3259"
     CDS             complement(4051..4989)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (39-182). E-value:
                     1.3e-08. Score: 26.2. Matches aSDomain:
                     nrpspksdomains_SAM23877_RS15355_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557970.1"
                     /locus_tag="SAM23877_RS15355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_3259"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_053132659.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.6e-17, bitscore:
                     61.6, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTLVITGAGGFIGGHVVREAERLGVTLRLTAHDARVRADGPRTT
                     VVRADLTDPGTLRGLCDGADAVLHCASLIGGPEERCEAVNARGTAALVDEARRAGVGRI
                     VQLSTAAVYGRGTFRRARPQDLRRAPGSATSRTRAAGEDAVLAAGGTVVRPHMVYGRGD
                     TRVVPALARLLRVLPGTVAGWQAQVSVIAAEDLARALVGVALAPAGHLTAPVYHACHPR
                     PVPWHTVLRTVAAVADVPWPERDLTLDQAYEALRARGGSRHDLDLLISDHWFDADPLWT
                     DLDTSPGPAFEEGFARHEDWYRRSLAPADGA"
     aSDomain        complement(4444..4872)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SAM23877_RS15355_NAD_binding_4.1
                     "
                     /evalue="1.30E-08"
                     /label="SAM23877_RS15355_NAD_binding_4.1"
                     /locus_tag="SAM23877_RS15355"
                     /protein_end="182"
                     /protein_start="39"
                     /score="26.2"
                     /tool="antismash"
                     /translation="DGPRTTVVRADLTDPGTLRGLCDGADAVLHCASLIGGPEERCEAV
                     NARGTAALVDEARRAGVGRIVQLSTAAVYGRGTFRRARPQDLRRAPGSATSRTRAAGED
                     AVLAAGGTVVRPHMVYGRGDTRVVPALARLLRVLPGTVA"
     gene            complement(5001..5363)
                     /locus_tag="SAM23877_RS41950"
                     /old_locus_tag="SAM23877_3260"
     CDS             complement(5001..5363)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /locus_tag="SAM23877_RS41950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_3260"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_053132661.1"
                     /sec_met_domain="AfsA (E-value: 2.5e-16, bitscore: 58.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSFTARPEAVGTAPERVGGQDSRDVVLAAADARSPHRRQPRVDTA
                     HPVCFDHPADHVPGMVLLEAAPQVAHAPTGPRDALPGGLESAFARYAPCGIDTVFTATA
                     TATATAPAAPCPTPFR"
     tRNA            5644..5716
                     /anticodon=(pos:3474169..3474171,aa:Arg,seq:cct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SAM23877_RS15365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SAM23877_tRNA15"
                     /product="tRNA-Arg"
     gene            5644..5716
                     /locus_tag="SAM23877_RS15365"
                     /old_locus_tag="SAM23877_tRNA15"
     misc_feature    5774..5788
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
     gene            complement(5788..>6284)
                     /locus_tag="SAM23877_RS15370"
                     /old_locus_tag="SAM23877_3261"
                     /pseudo=""
     CDS             complement(5788..>6284)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028858.1"
                     /locus_tag="SAM23877_RS15370"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="SAM23877_3261"
                     /product="tyrosine-type recombinase/integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="LDLDNRVLYVRQQTQRRRGVLYDDDPKRVAVAGPSRCPPSASRPC
                     AGTGCGRRLLGSRQGSSGRSPATSSPPVPAVRSSRGTSTAPSPASPSPPASA"
     gene            complement(6602..8344)
                     /locus_tag="SAM23877_RS37415"
                     /old_locus_tag="SAM23877_3262"
     CDS             complement(6602..8344)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008749770.1"
                     /locus_tag="SAM23877_RS37415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_3262"
                     /product="ABC-three component system protein"
                     /protein_id="WP_079030224.1"
                     /transl_table=11
                     /translation="MLRQLSADDPRFKTASFTSGMNLIVAEKRTSSTETDSRNSAGKSS
                     LIELIHFLLGARVDKRSLVSDRHLRSVNFHLKMDWPSNGVLTVGRRASRPNFVQLDPDI
                     RPGRQEEMFALGGPAEVTLDEWNKLIEKDLFGVRADASGVSGRTMLSFLMRRASAHGFN
                     QATESFSKQRKADAATNLAYLLGLDYELADGYRKLSAQKATRTQLRKAVNDPVWGRIVG
                     STADLRGQITLAEAQLRRLEDQIADFQVVPEYERVKQEADALARRIKQLAQDDTIDQSN
                     LEELQQAVTEASDVTVDYMESAYRELGILLGDQVRRRFDDVQTFHQSVVRNRRVFLEAE
                     IESLTSRLTERRDERARLGEQQAQLLRMLNEGGALEALTALQQALAREQANVEALRHRY
                     EAAQALEASARQITAQRLQQQQAVATDLNERREQQAEATLLFSRFARELYGEREAYLRI
                     EAGRENLQITPYIRSDNSRGIGNMVIFCFDLALSVLAHRHGRGPDFLVHDSHLFDGVDE
                     RQLVKAFALAAEVSAEEGMQYIATINSDDLAKAVRLGFNDAPFVREPRLTDDLEDGGLF
                     GFRF"
     misc_feature    complement(8441..8456)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.6"
                     /tool="antismash"
     misc_feature    8455..8469
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.24"
                     /tool="antismash"
     gene            complement(8585..9523)
                     /locus_tag="SAM23877_RS39075"
                     /old_locus_tag="SAM23877_3264"
     CDS             complement(8585..9523)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042705.2"
                     /locus_tag="SAM23877_RS39075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_3264"
                     /product="ABC-three component system protein"
                     /protein_id="WP_218922752.1"
                     /transl_table=11
                     /translation="MSHEQRLFARVRFLERMSELTGSAFEDFFHDLMCARYPDFLDVRT
                     HGNIGDQGADGLTLHDRKLFASYAPHAFSATDTKSKFNSDLKKAKEKRSGQFDVFVFVH
                     NDRRGMHPEIATLLADAKAANPALAFEQIGIRRLWQECMRLDLESAEDILDCEIPVRPV
                     VYGLGMEDLAPLLKHLRELRTKSDPLMPLDNVPPTKMDYNRLDEDAREDLQKGRRQAHL
                     VEAFYDGSLRAIEHDEVAEGFRIYYEQLKAEWDEPEEVLWQLEMYVLGNERQHPKVHRA
                     AWVVLAHFFDRCDIFETPPPGWTAQTLEASA"
     misc_feature    complement(8957..8959)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    9484..9495
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
ORIGIN
        1 gctgggccgc ggcgatcatc agatcgccca cgcccacgtc ggtacggagc tgaccggccg
       61 ccttcgcccg gtccatgacc tcggcgatga gccgctcgac ccgttcccgc gccgcttcca
      121 ggtcggggtg gtgctggtcg aaggtgctgg agaccatggg gcacagggcg ctgatccgct
      181 cgtcggcggc aacgtgcacg aagcgctcca gcgccccgaa ggcgtcgccg ttctccgcga
      241 gcgcgagctc ggccgcccgt gccgtacggt ccatgaccga gcagacgacc tcgcgtacga
      301 gggcgtcgcg gtcggggaag ttgcggtaca ccgtggcgtt gcccacgccg gcccggcggg
      361 cgatgtcgtc gagcggcacg tcggggccgt gctcgacgaa catctcgcgg gcggcggtga
      421 cgatccgctc ccgattgcgc agggcgtcgg cgcggggccg gctcaccttg tgctgctcgg
      481 gggttgcggt ctgcacggcg tactccttgg gcggtgggac tggcgatccg gggagggagt
      541 ccccgtttcg ctcggacaca tggctaaacg gggaaacgat ccccggttat ttccccgctc
      601 cggaaagaat caccgtgacc tgggtcacac cccgtcgact ccccctgccc tcctgcctcc
      661 ccaccccctc ttgcctctgc ttcctcaccc tcttgcccct gtcttcttgc ccctcttacc
      721 tctgtctcct tggcccccct tacctctgcc ttcttgcccc ctgttcggcg ccccgccctt
      781 caccggcccc tcccccggtc ggcggctacc ggcgagaaac ccacgatcgg ctcctccagc
      841 gcgcgcccgt cccgccctcg ccccagggtg agcgaaaggg tgcagccggt gaccgggggc
      901 tgccgtggtc cgggaggtct gcatgcagcc gcagccgcag ccgcagccgc cgagccgtcg
      961 gatacgcccg cgccggctgg ccgcgatcgc caccgtcacc gtcgtgacct tcgcggtcag
     1021 cacctcggcc ggcaccgggc ggctcgcgcc gggcaccacg gcggcgggcc ccggcggccc
     1081 ggccgccctc tcccggtcct ccgcgcacgg cccctgcatg atcaccggcg gtcccgagat
     1141 ccagatgtcc gagggcgtcc ccacctccgc cggctacgcc cgctccaccg gcaccgtccg
     1201 cgccctcacc ctgatgatcg acttctccga cgcacccggc gagggcgagg cgctcgaccg
     1261 ctacgcggag ttcttcccgc agaccgagga gtggttccgc accagctcct acggccgcct
     1321 cgactaccgt cccgagacgc cgctccgcga ctggctgcgg atgccgaagt cgttcgccga
     1381 gtacggcata gagcggggcg cacccttcga gcccggttac cgggagatgg tgcaggacct
     1441 ggtcgcggag gccgacgcga aggtcgactt ccaggagtac gacctcctga acgtgctggt
     1501 caccccgaac gccggcccct ccgccctgga caccgtcctg tccgtcacct tcgccggcaa
     1561 ccccgaggcc ccggtggccg acggcgtccc ggtctccaac gcgtccttcg tctactcccg
     1621 gcaggacgac ggctcgggct cctacgacga gaccggctac cgcgtcctcc cccacgagaa
     1681 tggccacgtc ttcggtctcc cggacctcta cacccaggag ggcgggggag ccgtcggcca
     1741 ctgggacatc atgagcgagg actggggcgc caacaacgac gtcctcggct ggcacaagtg
     1801 gaagctgggc tggctggacc ccgcccaggt gcactgcgcg tcggcgccgg ggacgcggga
     1861 gtacacgctg acgccgctgg cccgcaaggg cggcgacaag gtggtggtcg tcccgctgga
     1921 cggccgcagc gggtacgcgg tcgagctgcg cacccgcgag ggcaacgacg agacggtgtg
     1981 ccggccgggt gtgctgatct acaaggtcga cgcggacgtg gacaccggaa tgggcccggt
     2041 gacggtgttc gactcccgcc gggacagcgg cggctgcacc cgcagcccga acgtccacgc
     2101 cgaactctcg gacgccgcct tcgttccggg cgagaccttc gaggacccgc gccggggcat
     2161 cacgatcgcg gtgtcggggg cggacccgaa cggcgaccac aaggtgcggg tcacccgggg
     2221 gtgaacgacc ggggcgggcg ggcggtacgg acgcggggcc gccgtgcggc acgggcccgg
     2281 gccggccggg attacggtag gcctgccgcg atcgccgtac cggagagccg atgcccgccc
     2341 acaccacgaa cgacaccccg cgcgacgagg gtgtcgccgc cccggccgag gcggtcgccc
     2401 cgctgctgcg cggcatcgcc gtactgcgcc ggctgaccga ggcggacggc ggcaccctca
     2461 gtctcagcgc cctggagaag gcgaccggcc tggcccgctc cacggtcgac cgcgtcacgg
     2521 cgaccctcgc ccgcatggga tacgtccgcc tggacggccg cgacgtgacc ctcgcccccc
     2581 gcctgatgga gctgggcaac gcctacctgg ccgccctgcg cctgcccgcc ctgctctccg
     2641 cccgcgccga cgcactggcc gacgcactgg acgagtcggt ctccctcgcg gtcggcgacc
     2701 gcgacggcat ccgcttcatc caccaggcca cccgccgccg cgcgatgtcc ctcagcttcc
     2761 gcatcggcga cctgctcccc gccgaacgca ccgcgccggg cccgctgttc gccaccgagt
     2821 ggacggacgc cgactggcgc cgctggcacg accgcagggc gacggacccg cgggacacca
     2881 ccttcccggc cgtccccccg cgcgaacaca ccacccccga cgaggacttc gagcaccgta
     2941 cggcgcgggc ggcggccgac ggctgggcgc tggacgacca gttgatcgaa ccgggcctgg
     3001 tcgcggtctc ggtcccggta cgggaccccg gctccggccg ggtggcgtgc gtggcgagcg
     3061 tggtgagcca tacgagccgc cacaccgccg gtgacctgcg ggcggccctg ctcccccgcc
     3121 tgcgcacggc cgtcgcggcg atggaggacg acctgcgcac cgccccgccg ccggagccgg
     3181 gagcaccgcc cgcgggtctc gccgtctgga cgggagcgtc gaagcaggag ctgggccggg
     3241 agttcgtcga gtcactggcc cgcgggctga ccgtactgac cgccttcggc gagagccggc
     3301 cggcgccgac cctcacggag atcgcgaagg cgacgggcct ggcccgggcg accgtacgac
     3361 gggcgctgat caccttcgag cacgcggggc tggtcgcacc ggcccccggc cgcgccttca
     3421 cgctcacccc ccgggtgctg tccctcgggt tcccgccgct ctcccgcgcc tccctgccgg
     3481 agatcgccca gccccacctg accgcgctcg ccgaccgcgt gcgcgagccg gccgccctgg
     3541 ccgtcctggc ggactcgggc gaggagatcc agtacacggc ccgggcgtcc acgagccacg
     3601 tactgagcgc ccacgtcacg gtcggcgccc ggcgcccggc gtacgcgacg gcaccgggcc
     3661 gcgtactgct cgccggcctg cccgccgacc ccctccccga ggtccgggcc cagggccacg
     3721 ccctggtcga cgaggagctg gaggcgggtc tgcgctccct cgcggtcccg gtgcgcgacc
     3781 gtacgggccg ggtggtggcg gccgtgaacg tggcgacgca cgcggcccgc aggagcgcag
     3841 tgtcctgcgt ggcggacgtc ctgccggaac tgcggcgcac ggccgacctc gtcgaggcgg
     3901 acctgcgggt ggcggggcgg ttcaggacgg tgccgacgac ctgacccgga cgccggccct
     3961 ggcccgtccg cccggtcccg acctcgcggc ccgtcccgtg gctcaacgag ggctgcgcgt
     4021 gccgtacggc gggccaggct cccggcgggg ctaggccccg tccgccgggg ccagtgaccg
     4081 ccggtaccag tcctcgtgcc gggcgaagcc ctcctcgaac gccggacccg gcgaggtgtc
     4141 gaggtccgtc cacagcgggt cggcgtcgaa ccagtggtcg ctgatcagca ggtcgaggtc
     4201 gtgccgggag cccccgcggg cccgcagtgc ctcgtacgcc tggtcgaggg tcagatcgcg
     4261 ctcgggccac gggacgtccg cgacggcggc caccgtacgc agcaccgtgt gccagggcac
     4321 gggccgcggg tggcaggcgt ggtagacggg cgcggtgagg tgcccggcgg gtgcgagagc
     4381 caccccgacc agggcacggg cgaggtcctc ggcggcgatg accgacacct gcgcctgcca
     4441 gcccgccacc gtgccgggca ggacccgcaa cagccgggcc agcgcgggca ccaccctcgt
     4501 gtccccgcgg ccgtacacca tgtgggggcg gacgaccgtg ccgccggcgg ccagtacggc
     4561 gtcctcgccg gccgcccggg tccgcgaggt ggccgacccc ggggcccgtc gcaggtcctg
     4621 cgggcgagcg cgccggaagg tgccccgccc gtacaccgcg gcggtgctga gctggacgat
     4681 ccgcccgaca cccgctcggc gggcctcgtc gacgagcgcg gccgtgccac gggcgttgac
     4741 cgcctcgcac cgttcctccg ggccgccgat cagggaggcg cagtgcagca cggcgtcggc
     4801 gccgtcgcac aggccgcgca gcgtgccggg gtcggtgagg tccgcgcgga cgaccgtcgt
     4861 acggggcccg tccgcgcgca cccgggcgtc gtgggccgtc agccgcaggg tgacgccgag
     4921 gcgttcggcc tcccggacga cgtgcccgcc gatgaagccg ccggcaccgg tgatcacgag
     4981 ggtggacacg ggccgcctcc tcagcggaac ggggtcgggc acggggccgc gggcgcggtt
     5041 gcggttgcgg ttgcggttgc ggtgaacacc gtgtcgatcc cacagggcgc gtagcgcgcg
     5101 aaggcgctct cgaggccgcc gggcagggcg tcgcgcgggc ccgtcggggc gtgcgcgacc
     5161 tgcggggccg cctccaggag caccatgccg ggcacgtggt cggccggatg gtcgaagcag
     5221 accgggtgcg cggtgtccac ccgcggctgc cggcggtgcg gggagcgcgc gtcggccgcg
     5281 gccaggacga cgtcgcggga gtcctgtccg ccgacgcgtt ccggcgctgt gccgaccgct
     5341 tccggccggg cggtgaacga catgggtcgg atcatatccc tggcgatccg tcagatcctc
     5401 aacaacggtg acgaggaggc cgcccgatcc ccgactcccc gacctcgccg gcctgacccg
     5461 ttcacaccgc acacacaccc ggcgcccacc cgaggaggca gcacccgccc gagccccgat
     5521 ccggtaccct gtggatcgag cgccccggcg ggcgctgcga cacctgcccg ggcccacccc
     5581 ggaccccgtc gcagccgccg gccccgggac actcagggct tgacggactt ccgctcaagc
     5641 cccgccttcg tagctcaggg gatagagcac cgctctccta aagcgggtgt cgcaggttcg
     5701 aatcctgccg ggggcacaag ggagaaggcc acctaggaac gttcctaggt ggcctttgac
     5761 atccacttct gacatcaacg cgggcgatca cccacgatca gggcgccgct tcctgagcag
     5821 ccggtccatg tgactcatgg cctcgcgctg cgtgtcctgc acaacgtgtg tgtagacgtc
     5881 catggtgatg ctgatctgag agtgcccgag gatctccatc acgacgcggg gtgcgactcc
     5941 ggccgccgtg aggagtgtgg cggtgccgtg ccgggcgtca tgcagccgga tcacgcggag
     6001 gccggcggac tcggcgacgc gggtgaagga gcggtagacg ttccgcggct cgacctgacg
     6061 gccggtacgg gtggtgaaga cgtagccgga ctcctgccac tgctcccctg ccttgacccg
     6121 agcagccgcc tgccgcatcc ggtgccagcg caggggcgcg atgcagaggg cgggcagcgg
     6181 gacggcccgg cgacggcgac tctcttgggg tcatcgtcgt agaggacacc ccgccggcgc
     6241 tgcgtctgct gacgaacgta gagaacacgg ttgtcgaggt caaggcccgc gcacggcgcg
     6301 ggcgcgcgcc gcctctgcgg cgcggcctgc ctcctgtctg cgccccggct ggccgcgccc
     6361 gacggctgcg ggcatgaagt gggagaacac cctctgtggc tggggcggtc ggctccacgg
     6421 ctttaggcag ccactttggg gcgagcctct aagatcttgg ccccaacgtc gtgctttaac
     6481 gggcaggtcc acagactgcc cgactcgcca tcagcttacg gggccaagat cactcgcccc
     6541 aaagcagctc cagctcaaag ccgctccacc gaccttgcaa gatgagctcc gccgtatgcg
     6601 cttagaagcg gaatccgaaa agacctccgt cttccaagtc gtccgtcaga cgcggttcgc
     6661 gcacaaacgg agcatcattg aaccctaggc ggactgcctt agctaggtcg tcagagttaa
     6721 tcgtggcgat gtactgcatt ccctcttctg cacttacttc ggcggccagt gcgaaggcct
     6781 taaccagttg ccgctcatca acgccgtcga agagatggct atcgtgtaca aggaagtctg
     6841 ggccacgccc gtgacgatgc gctagaacag aaagggcaag atcgaaacaa aagatcacca
     6901 tgtttccgat gccgcgactg ttgtcgctgc gaatgtacgg agtgatttgg agattttcac
     6961 gacctgcctc gatgcgcaga taggcctctc gttcgccgta caactcccgg gcgaatcgtg
     7021 agaacagcag cgttgcttcg gcttgctgct cacgtcgctc attaagatca gttgcgacag
     7081 cttgctgttg ttgcaaacgt tgggccgtga tttgccgcgc gctcgcttcg agtgcttgag
     7141 cggcttcata cctgtgacgc aatgcctcga cattcgcctg ttcgcgagcc aaagcctgtt
     7201 gaagagccgt cagagcttcg agagctccgc cttcattcag catgcgcaaa agctgcgctt
     7261 gttgctcgcc gagcctagcg cgctcatccc gccgctcagt caatcggctg gttaggctct
     7321 cgatttcagc ttcaaggaaa actcgacggt tccgcaccac agattgatga aaagtttgca
     7381 catcgtcaaa ccgtcgccgg acttgatccc ccagcaaaat acctagttcg cggtaggccg
     7441 actccatgta atccacagtg acgtcgctgg cctccgtgac agcttgctgc agctcttcaa
     7501 ggttgctctg gtcaatggtg tcatcctgcg caagctgctt gattcgacga gctagtgcgt
     7561 ccgcttcctg cttgacccgt tcgtattcgg gaaccacttg gaaatcggca atctgatctt
     7621 caaggcgacg caattgtgcc tcagcgaggg tgatctgacc gcgcaggtct gcggttgaac
     7681 cgacaatccg accccatacc ggatcattga ctgcctttcg caactgcgtg cgagtggctt
     7741 tttgtgcgga gagtttacga tatccatcag caagttcgta atccaggccc aaaagataag
     7801 ctagattagt tgcggcatcg gctttccgct gcttggaaaa actctccgtt gcttgattaa
     7861 acccgtgtgc ggacgcccgt cgcattagga acgacagcat ggtgcgaccg ctcacgccag
     7921 aggcgtctgc gcgaacacca aataggtcct tctcgatcag cttgttccac tcatcaagcg
     7981 tgacctctgc cggccccccg agcgcgaaca tctcctcttg cctgccaggg cggatgtctg
     8041 gatcaagttg cacaaaatta ggccgactag cccggcgccc cacagtcaga acgccattac
     8101 tcggccaatc catcttcagg tgaaagttca cactccgaag atgcctgtca ctgactagtg
     8161 aacgcttgtc tacgcgtgcg cctagaagga agtggatgag ctcaatgaga cttgattttc
     8221 cagcactatt acgactgtca gtctcggtgg aggaagtcct cttctcagcc acaatcaggt
     8281 tcattccgct ggtgaatgat gcggttttga accgcgggtc atcagcgctg agctgacgca
     8341 gcatggatcc tcctcgctac aagcaagtcc tggcgaagct ctacggcgcc caagctgtac
     8401 agcatatcta gtgctaggac gaaccattcg aacgacaccg gagaaaagtg ttcattctca
     8461 atccgccatg cctttagccg cgcccacgcc tgactgacag tacggggctc ttcaagctgc
     8521 atgaggatct gggcgccaac ggccaacagg catcggtcgg gggaaattcc cttcgtcgga
     8581 gtaatcatgc agatgcctca agggtttgcg ccgtccatcc cggcggtggg gtttcaaaga
     8641 tgtcgcaccg gtcgaagaag tgagctagta ctacccacgc tgcgcgatgc accttgggat
     8701 gctgtcgctc atttccgaga acgtacattt ccagttgcca caggacttcc tcaggctcgt
     8761 cccactcggc cttcagttgc tcataataga ttcggaaacc ttctgcaact tcgtcgtgct
     8821 cgatagcgcg caacgacccg tcgtaaaagg cctcgacgag gtgagcctgt cgtctcccct
     8881 tctgtaggtc ttctcgggcg tcttcatcca gacggttata atccatcttg gtaggcggca
     8941 cattgtccag aggcattaag ggatcagact tcgtcctaag ctccctcaag tgcttcaaca
     9001 atggggcaag gtcttccatt ccgaggccat acaccactgg gcgtacgggt atttcgcagt
     9061 caaggatatc ttccgcagac tcgaggtcca gccgcataca ctcttgccaa aggcgccgga
     9121 tccctatctg ttcgaacgca agagcgggat ttgcggcctt ggcgtcagcc agaagtgtcg
     9181 ctatctcggg atgcattcct ctgcggtcgt tatgaacaaa aacaaagacg tcgaattgac
     9241 ctgaccgttt ttcttttgcc ttcttcaaat ccgaattgaa ctttgacttg gtgtcggtcg
     9301 cactgaaagc gtgaggtgcg tagctggcga acaatttcct gtcgtggagt gtcagcccgt
     9361 ccgccccttg gtctccgata tttccgtgcg tgcgcacgtc gagaaaatcg ggatagcgcg
     9421 cgcacatcaa atcgtggaaa aagtcttcga aagccgaacc ggtcaactcg ctcatgcgtt
     9481 caagaaagcg aacgcgcgcg aacagtcgtt gctcgtggct catcctcata cagccccctc
     9541 acccctgcag atcacggtag ctcttcgtga tacccgctgg aaaccgggtg ggcacaactt
     9601 gtcacgagct gagttcatcg ggtgatcgga gggggacggt gccgacaagc gatggaggcc
     9661 aagtgaagag gcgtacaggc cgaggcggag ccctcgcagc gacggtggaa ccttccctca
     9721 gggtgttggt gcaacgcccc cgacgcatgg agcagccaag gattggccgt catcgctgcc
     9781 gaacgcccgt ctggcagcac ccccgacacg accgacggcc cggcgcctgg cgtcgcccgc
     9841 agcgtgcacg acctcagcag tggcggcacc agcgcgttcg ttgaggaccg ctgcgaactc
     9901 gcgatcaaga ctccagcgag cctcagtagc ggctactgcg acgctgctgg ggcctgcgac
     9961 actcaagact gcttgagctc ggatgagctc tgacaaccca gcctgcgctg cctccaatcg
    10021 atcttgatgc gcctcttcgg ccatgccggg tgatctggtc cgctggacga gcgcagcgca
    10081 gcggatggcc gtctctatga actggataca tgcgtccgtc tgcgccgtcc ggctctcctc
    10141 tgcccgccgc tcggcgtgat cctgcgctgc gacttctcgt tgcacctctg cctggcgaag
    10201 tacgccccga agtgccaacc atgaggtcat acctgaggcc acgagggcag tgactgccgc
    10261 aatgactgcg gtgagaagtg aatcgctcac aaggggattg catcagagag acgcactgcg
    10321 gcgcaggcgg tcaggggtct ggctggcttc ctcgtcgggc cgt
//
