LOCUS       NZ_CP012382            10528 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Streptomyces ambofaciens ATCC 23877 chromosome, complete genome.
ACCESSION   NZ_CP012382
VERSION     NZ_CP012382.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces ambofaciens ATCC 23877
  ORGANISM  Streptomyces ambofaciens ATCC 23877
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8303940)
  AUTHORS   Thibessard,A., Haas,D., Gerbaud,C., Aigle,B., Lautru,S.,
            Pernodet,J.-L. and Leblond,P.
  TITLE     Complete genome sequence of Streptomyces ambofaciens ATCC 23877, the
            spiramycin producer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8303940)
  AUTHORS   Thibessard,A., Haas,D., Gerbaud,C., Aigle,B., Lautru,S.,
            Pernodet,J.-L. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Faculte des Sciences et Technologies,
            Universite de Lorraine, Boulevard des Aiguillettes, Vandoeuvre les
            Nancy F-54506, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC workbench v. 7.0.3
            Genome Coverage                   :: 114
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001267885.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 02:57:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,510
            CDSs (total)                      :: 7,418
            Genes (coding)                    :: 7,242
            CDSs (with protein)               :: 7,242
            Genes (RNA)                       :: 92
            rRNAs                             :: 5, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 74 of 176
            Pseudo Genes (incomplete)         :: 131 of 176
            Pseudo Genes (internal stop)      :: 12 of 176
            Pseudo Genes (multiple problems)  :: 39 of 176
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:42:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2873095
            Orig. end                         :: 2883623
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012382.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10528
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="8"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10528
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10528
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5528](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5528)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(123..138)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.27"
                     /tool="antismash"
     gene            complement(141..1748)
                     /locus_tag="SAM23877_RS12830"
                     /old_locus_tag="SAM23877_2726"
     CDS             complement(141..1748)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435092.1"
                     /locus_tag="SAM23877_RS12830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2726"
                     /product="dynamin family protein"
                     /protein_id="WP_053131048.1"
                     /transl_table=11
                     /translation="MVTLDVRPQLLDALSALRDRVAAARFPLPLAGAPRARANRDELLA
                     QLDDYLVPRLKDPEAPLLAVVGGSTGAGKSTLVNSLVGRRVSEAGVLRPTTRTPVLVCH
                     PEDHHWFSGMRVLPDLTRVWVPHREPDDDLLLPGESPARVLRVETADSLPPGVALLDAP
                     DIDSLVADNRVLAAELICAADIWVMVTTAARYADAVPWHLLRTAKEYDATLVTVLDRVP
                     HQVVSEVSRQYGALLAKAGLGEVPRFTVPELPESAWGGGLLPASAVAPLRAWLVHRAQD
                     LDARHNAMARTAHGILDSLKSRMPELAGAAAAQYSAALRLTAAVDGAYDGEHARVRGRL
                     QAGAVLAGDALKRWRAFPLDCSSEELLDSLVESLSALLLCAVTAADERVDAAWRGEPAA
                     TAPELTGHDPTPESAEHRIGMAVRRWRRELEEYAEEEVRRLDRSLAPDPGVVTALVATV
                     LLGGRRARSAGEGLAERIGAHGALRLRDRGGRLLDDCVDRVVHVERERRLAPLDGMEVH
                     PEPQAELIAALSVLQKER"
     misc_feature    complement(1759..1775)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.58"
                     /tool="antismash"
     misc_feature    complement(1764..1778)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.96"
                     /tool="antismash"
     tRNA            2079..2154
                     /anticodon=(pos:2875207..2875209,aa:Arg,seq:tct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SAM23877_RS12835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SAM23877_tRNA9"
                     /product="tRNA-Arg"
     gene            2079..2154
                     /locus_tag="SAM23877_RS12835"
                     /old_locus_tag="SAM23877_tRNA9"
     gene            2185..3203
                     /locus_tag="SAM23877_RS12840"
                     /pseudo=""
     CDS             2185..3203
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449723.1"
                     /locus_tag="SAM23877_RS12840"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /pseudo=""
                     /sec_met_domain="2-Hacid_dh_C (E-value: 3.4e-23, bitscore:
                     80.6, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQRVATEIADWSPVEGDVDVVSFDTHHADEETLAA
                     ALADVDIVVTLRERVPFPGSLITRLPRLKLIIASGMRNSVIDYAAAEAHGVTVCGTASS
                     STPPVELTWTLLLGLARGIVQESNALREGGPWQQTVGADLHGRRLGLLGLGKIGGRVAQ
                     VGLAFGMRVSGWSQNLTRERADEVGVELAPSKEDLLRTADFVSVHLALGDRTRGLLGAA
                     ELALLSRPPTWSTPRARRSSTRTPCSPRCTRGGSPARASTSSTPSRCPPDTRCAPPRAC
                     WRPRTSATSRRPTTPRTTARRSRRSRPTSRGPPYGGCPRPARRTLRRLPPGRMAPDAS"
     gene            complement(3161..>3439)
                     /locus_tag="SAM23877_RS12845"
                     /old_locus_tag="SAM23877_2728"
                     /pseudo=""
     CDS             complement(3161..>3439)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449721.1"
                     /locus_tag="SAM23877_RS12845"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2728"
                     /product="DUF2087 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="LIVRTFVKDGRLVRLPARWTRKKVVLRHIAERTFVPGAEYPERTV
                     DAKLRAWCDEGGVDHVTLRRHLVDLGHLCRSGGTYWRPAPSGRAGAV"
     misc_feature    complement(3507..3521)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.67"
                     /tool="antismash"
     misc_feature    complement(3636..3651)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 16.87"
                     /tool="antismash"
     gene            3753..4061
                     /locus_tag="SAM23877_RS12850"
                     /old_locus_tag="SAM23877_2729"
     CDS             3753..4061
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449724.1"
                     /locus_tag="SAM23877_RS12850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2729"
                     /product="chaplin"
                     /protein_id="WP_053131061.1"
                     /transl_table=11
                     /translation="MSRIAKAAGVVLGAGAVVLSGTGLAMADAGAEAAAIGSPGVLSGN
                     VVQIPVNVPVNVCGNTINVIGLLNPAFGNACENGDDDKKKDESDKHGDDGKNSGYGS"
     gene            complement(4151..5017)
                     /locus_tag="SAM23877_RS12855"
                     /old_locus_tag="SAM23877_2730"
     CDS             complement(4151..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="SAM23877_RS12855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2730"
                     /product="tyrosinase family protein"
                     /protein_id="WP_053131064.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRTEKRRFVKALLEIKRRGEYDEFVRTHIEYYVS
                     DGEDGLRTAHMAPSFLPWHRRFLLDLEEALRRVDPSVTVPYWDWTKDRTTSSAPWTADL
                     LGGTGRRSDHRVTTGPFAHAEGNWTLKENVTDTEYLTRDLGRAADPLGLPTKSDLEWAL
                     DDPVYDTTPWDSTSRKGFRNKLEGWGTGRGSVSWRNHNRVHRWVGGAMVGGASVNDPVF
                     WLHHAFVDLLWSRWQARHRGARYLPAEPPGRGSAQHGRIVARHEKLPPWDVTPDELEDH
                     SRIYRYA"
     gene            complement(5001..5528)
                     /locus_tag="SAM23877_RS12860"
                     /old_locus_tag="SAM23877_2731"
     CDS             complement(5001..5528)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660312.1"
                     /locus_tag="SAM23877_RS12860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2731"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_053131067.1"
                     /sec_met_domain="melC (E-value: 1.5e-50, bitscore: 169.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVNLGGTPSGTEREAEGPARGRPAHGTRRQMMRGLLAPALAVGL
                     APLVVASRPSRAAATGAGGQPARPEPRAEEPSGSASFDETYRGRRIRGIRSAAGRAVGA
                     GSWHVTVDGRPLHLMRRADGSWLSMIDHYRSYPTPLAAARGAVDELGPGEHLRDTPAAG
                     QEHSGGHHGVHA"
     gene            complement(5659..6174)
                     /locus_tag="SAM23877_RS12865"
                     /old_locus_tag="SAM23877_2732"
     CDS             complement(5659..6174)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="SAM23877_RS12865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2732"
                     /product="hypothetical protein"
                     /protein_id="WP_053131070.1"
                     /transl_table=11
                     /translation="MKSTTRRTLAAVVTGVAAAVGVAAAPAAAAGAIPVPVPLGGAETA
                     LGMELPDVAGELPVLTPGAPEGPRYVEGRLLPERTLPQLPVRSGLPGVDLRQPLPQVLG
                     DGFDHAAVEAPATELRALAPGLSLDAPLSAPDGGSFGLPAAQLPQAGVLTPVVQTVPAA
                     DLGVGPGL"
     misc_feature    6192..6206
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.52"
                     /tool="antismash"
     gene            complement(6240..6521)
                     /locus_tag="SAM23877_RS12870"
                     /old_locus_tag="SAM23877_2733"
     CDS             complement(6240..6521)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325462.1"
                     /locus_tag="SAM23877_RS12870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2733"
                     /product="hypothetical protein"
                     /protein_id="WP_053131080.1"
                     /transl_table=11
                     /translation="MKSLKAAALVAGSLVAAGSAAPAFAYDAEALTPTSLNGAVNALTA
                     EPIDVMPLKHQSEALDTENKDSVLHTVKGATTQLNSKGGLLGGLPLGR"
     gene            complement(6742..7500)
                     /locus_tag="SAM23877_RS12875"
                     /old_locus_tag="SAM23877_2734"
     CDS             complement(6742..7500)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976096.1"
                     /locus_tag="SAM23877_RS12875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2734"
                     /product="hypothetical protein"
                     /protein_id="WP_053131083.1"
                     /transl_table=11
                     /translation="MRLPARRIATTALTAGLLIGLSGPAVMAADGDSVRERTHAASRAP
                     LPDVEELQDQVGSLAGLGGVLTPVTDLLSAVLKADDGRLSATDADQLSAAVKDALAKAD
                     AADADADDTATTPGTTTPGTATPGTSTPAQPPAVTAPEAETPVTLPAPVVAQDSDGTAA
                     ADALAADAHAALQKQIDALVQAATSGTAEQVTPAVQEVVNGVVNVVAATLVGSGLPAAD
                     LAGLPALPSAPEAATPEAAAPANPLPADPA"
     gene            complement(7802..8041)
                     /gene="chpG"
                     /locus_tag="SAM23877_RS12880"
                     /old_locus_tag="SAM23877_2735"
     CDS             complement(7802..8041)
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="SAM23877_RS12880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2735"
                     /product="chaplin ChpG"
                     /protein_id="WP_053131086.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVAGTAGVAAADAGAQAAAAHSPGVLSGN
                     VLQVPVHIPVNVCGNTIDVIGLLNPAFGNECEND"
     misc_feature    complement(8110..8126)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.15"
                     /tool="antismash"
     gene            8432..>9646
                     /locus_tag="SAM23877_RS12885"
                     /old_locus_tag="SAM23877_2736"
                     /pseudo=""
     CDS             8432..>9646
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325465.1"
                     /locus_tag="SAM23877_RS12885"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_2736"
                     /product="glycosyltransferase"
                     /pseudo=""
                     /sec_met_domain="Glycos_transf_1 (E-value: 8.9e-23,
                     bitscore: 79.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLPPTESRPRVLHITQPVEGGVARVVADLTRAQLAAGLRVAVAC
                     PDGDLADRLRSLGADVRHWPATRSPGPALFPEVRRLARVVREVRPDLVHAHSAKAGLAG
                     RLAVRGRVPTVFHPHAWSFEAVDGAAAALALGWERWGARWAARTVCVSEAERLRGARAG
                     VRARWSVIPNGIDLDRFGADDVAEQAPLVDVGTAGPDAPLVVDAGTAGPDAPLVVCVGR
                     LCRQKGQDVLLSAWESVLARVPGARLVLVGDGPDRARLTERASAGRPAPPGPGDGHPGE
                     SGPASVLFTGAVVDAVPWYRAADLVVLPSRWEGMALAPLEAMACGRPVVVTDVDGARES
                     LPPALVPHCVVPPENPAALADAVARLLLDAPLRTSLGRRGRAHVRSTHDVRHTAAAVAA
                     LYDGLL"
     misc_feature    complement(10073..10087)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.33"
                     /tool="antismash"
ORIGIN
        1 cggtccactt cacccggccg gtccacttca cccggccggt ccacctcgcc cggtccggcc
       61 gcctgagccg gtcggtccgc ctcctccgtc gtgtcgtggg tgtgctcggt ggggtcctgg
      121 tcagtgacgg cggtcaccgg tcacctctcc ttctgcagta cggacagcgc ggcgatgagt
      181 tcggcctggg gttcggggtg gacctccatc ccgtcgagcg gtgccagccg gcgttcgcgc
      241 tcgacgtgca cgacccggtc cacgcagtcg tcgagcaggc gtccgccccg gtcccgcagc
      301 cgcagcgccc cgtgggcgcc gatgcgttcg gcgagcccct cccccgcgct gcgcgcccgg
      361 cgcccgccga gcagcacggt ggcgaccagg gcggtgacca caccggggtc cggcgccaga
      421 ctccggtcga ggcggcgcac ctcctcctcg gcgtactcct ccagctcccg acgccaccgc
      481 cgcaccgcca tcccgatgcg gtgctcggcg ctctccggcg tggggtcgtg gcccgtgagt
      541 tccggggcgg tggccgcggg ttcgccccgc catgccgcgt cgacccgttc gtcggcggcg
      601 gtcacggcac acagcagcag ggcgctcagg ctctccacca gcgagtcgag gagttcctcg
      661 gaggagcagt cgagggggaa ggcacgccac cgtttgagcg cgtccccggc gagtacggcg
      721 cccgcctgca accgcccccg cacgcgcgcg tgctcgccgt cgtaggcacc gtcgacggca
      781 gcggtgagcc gcagcgcggc ggagtactgt gcggcggccg caccggccag ttcgggcatc
      841 cgggacttga gcgagtccag gatgccgtgc gcggtacggg ccatggcgtt gtgccgggcg
      901 tcgaggtcct gcgcccggtg gacgagccag gcccgcagcg gggccaccgc gctggcgggc
      961 agcagccctc cgccccaggc cgactcgggc agctcgggga cggtgaagcg gggaacctcg
     1021 ccgagcccgg ccttggccag cagcgccccg tactgccgtg acacctcgga caccacctgg
     1081 tggggcaccc ggtcgagcac ggtgaccagg gtggcgtcgt actccttggc ggtgcgcagc
     1141 agatgccagg gcacggcgtc ggcgtagcgg gcggccgtgg tgaccatcac ccagatgtcg
     1201 gccgcgcaga tcagctcggc cgccaggacg cggttgtcgg cgacgaggga gtcgatgtcg
     1261 ggcgcgtcga ggagggcgac gccgggcggc aggctgtcgg cggtctccac acgcagtacg
     1321 cgcgcggggc tctcccccgg cagcagcagg tcgtcgtccg gttcccggtg gggcacccac
     1381 acgcgcgtga ggtccggcag cacccgcatg ccgctgaacc agtggtggtc ctccggatgg
     1441 cacacgagca ccggcgtccg ggtggtcggc cgcagcacac ccgcctcact gacccgccgg
     1501 cccaccaggg agttcaccag tgtggacttc ccggcgccgg tggagccgcc caccacggcc
     1561 agcaggggcg cttcgggatc tttcaaccgg ggcaccaggt agtcgtcgag ctgtgcgagg
     1621 agttcgtcac ggttggcacg cgcgcgtgga gcccccgcca ggggcagcgg gaagcgtgcg
     1681 gccgcgacac ggtcgcgcag ggcggagagt gcgtcgagca gctgaggccg tacgtccaag
     1741 gtcaccacat gtgaagaatg cccaatttta ggggaattct gaagcatatg ggcatgatga
     1801 gtatgtccgc gcgccgatcg gacactccgg acggaaggga ttaccggggc gcaaggacag
     1861 tccaggcata acgagtgcac aacacccgat gcgtgagacg ccaaaagcga tgcacgattc
     1921 gcacctgcct gcgattatca ggaccgcttc accgaacctc cacatcgagc cacggaggcg
     1981 gagcagcagg gacacggagc cgggagccct atccttgtcc ccggcaacgt cacggaacag
     2041 cccacacccg ggcaccgaga acgaggccac cacaccaggc ccccgtagct cagtggatag
     2101 agcaggcgcc ttctaagcgc ttggccgcag gttcgagtcc tgccgggggc gccactgccc
     2161 gttgggcggg agggacggga cgacgtgcga ctgcgctgcg cggtgctcga cgacttccag
     2221 cgagtggcga ccgagatcgc cgactggtcg cccgtcgagg gcgacgtgga cgtcgtgtcc
     2281 ttcgacaccc atcacgccga cgaggagacc ctcgccgcgg ccctcgccga cgtcgacatc
     2341 gtggtcaccc tgcgggaacg cgtgccgttt cccgggtcac tgatcacccg actgccccgg
     2401 ctgaaactga tcatcgcctc cggcatgcgc aacagcgtca tcgactacgc cgccgccgag
     2461 gcgcacggcg tcaccgtgtg cggtacggcc tcctcgtcga cgccacccgt cgaactgacc
     2521 tggaccctgc tgctgggcct ggcccgcggc atcgtccagg agagcaacgc gctgcgggag
     2581 ggcggcccct ggcagcagac ggtgggagcc gacctgcacg gccgccggct cggcctgctg
     2641 ggcctcggga agatcggcgg ccgggtggcg caggtcgggc tcgccttcgg catgcgggtg
     2701 agtggctgga gccagaacct cacccgggag cgcgccgacg aggtcggtgt cgaactcgcc
     2761 ccctccaagg aggacctgct ccgcaccgcc gacttcgtct ccgtccacct ggcgctcggc
     2821 gaccgcaccc gcggcctgct aggcgccgcc gagctggccc tgctcagccg accgcctacc
     2881 tggtcaacac ctcgcgcgcg gcgatcgtcg accaggacgc cctgctcgcc gcgctgcacg
     2941 aggggcggat cgccggcgcg ggcgtcgacg tcttcgacac cgagccgctg cccgccggac
     3001 acccgatgcg caccgccccg cgcctgctgg cgaccccgca cctcggctac gtctcgcaga
     3061 ccaactacgc cacgtactac ggccaggcgg tcgaggcgat ccaggcctac ctcgcggggt
     3121 cccccgtacg gcggctgccc gcgccccgcg cgtcggacgc tcagacggct cccgcccggc
     3181 cggatggcgc cggacgccag taggtcccgc cgctccggca cagatggccc aggtccacca
     3241 ggtggcgccg gagcgtcaca tggtcgacgc cgccctcgtc gcaccaggcg cgcagcttcg
     3301 cgtcgaccgt gcgctccggg tactcggcgc cgggcacgaa ggtccgctcg gcgatgtgtc
     3361 gcagcaccac cttcttgcgc gtccagcgcg cgggcaggcg taccagccgt ccgtccttga
     3421 cgaaggtccg cacgatcagg ccggcccggt cgtcctcggc cccgggggtg ccgtcgggcg
     3481 gcgccgtgga ccggccctcg cgggccagtt ccctgaaaag atcgcggcgt tcggatcggt
     3541 cgtcaaccat gccgggaggc tggtggacgg cccggctccg ggtggagcgg ttttcccgcc
     3601 cgccggcacc ggcgcgcccc cttccgcagc cccctgttca aaaagggtga tcctcacatt
     3661 ccccccgtga ctcctctccg acctggtgcg ttgaaccggc cgtgcggtgg cacgtcccgc
     3721 tgccgtgctc cccgagtcag aaggagaacg tgatgtctcg catcgcgaag gccgccggcg
     3781 tcgtactcgg cgccggtgcg gtcgtgctca gcggcaccgg ccttgccatg gcggacgcgg
     3841 gcgccgaggc cgcggccatc ggttcgcccg gtgtcctgtc cggcaacgtc gtccagatcc
     3901 cggtgaacgt gccggtcaac gtctgcggca acacgatcaa cgtgatcggt ctgctgaacc
     3961 cggccttcgg caacgcctgc gaaaacggcg acgacgacaa gaagaaggac gagagcgaca
     4021 agcacggcga cgacggcaag aacagcggct acggcagctg acccctgcgg tcaccgagga
     4081 gccccggcca ccatcggccg gggctcctcc cgttcccccg gttccgtccc tccggcgtcc
     4141 tcaggcgcgc tcaggcgtac cggtagatcc ggctgtggtc ctccagctcg tccggcgtca
     4201 cgtcccaggg cggcagcttc tcgtgccgcg cgacgatcct tccgtgctgt gcgctgcccc
     4261 ggccgggcgg ttcggcgggc aggtagcggg ccccgcggtg ccgggcctgc cagcgggacc
     4321 acaggaggtc cacgaaggcg tggtgcagcc agaacaccgg gtcgttgacg gaggcgccgc
     4381 cgaccatggc cccgccgacc cagcggtgca cacggttgtg gttgcgccag gagacgctgc
     4441 cgcggccggt cccccacccc tccagcttgt tgcggaaccc cttgcgggac gtggagtccc
     4501 agggcgtggt gtcgtacacc gggtcgtcga gggcccactc caggtcgctc ttcgtgggca
     4561 gccccagggg atcggcggcc cgtccgaggt cccgggtgag gtactcggtg tcggtgacgt
     4621 tctccttgag ggtccagttg ccctcggcat gggcgaacgg cccggtggtc acccggtggt
     4681 cggagcgccg cccggtgccg ccgagcagat cggcggtcca gggggcggag gaggtggtgc
     4741 ggtccttcgt ccagtcccag tagggcacgg tgaccgacgg gtcgacccgg cgcagcgcct
     4801 cctccaggtc cagcaggaac cggcggtgcc agggcaggaa ggacggcgcc atgtgcgccg
     4861 tacggagacc gtcctcgccg tcggagacgt agtactcgat gtgggtgcgc acgaactcgt
     4921 cgtactcacc acgccgtttg atctccagca gcgccttgac gaaccgtcgc ttctcggtgc
     4981 gggtgagggt gctgacgtcc ttacgcgtgt acgccatggt gccccccgga gtgctcctgg
     5041 ccggcggccg gggtgtcgcg caggtgctcg ccgggaccga gttcgtccac ggccccgcgc
     5101 gccgcggcga ggggggtggg gtacgagcgg tagtggtcga tcatgctgag ccagctgccg
     5161 tcggcccggc gcatcagatg caggggccgg ccgtccacgg tgacgtgcca ggaaccggcg
     5221 ccgacggcgc gccccgcggc ggaccggatg ccgcggatgc ggcggccgcg gtaggtctcg
     5281 tcgaacgagg cgctgccgga cggctcctcg gcgcgcggct cggggcgcgc gggctgcccg
     5341 cccgcgccgg tggcggcggc ccgggagggc cgggaggcga cgaccagggg cgccaggccc
     5401 acggcgagcg ccggggcgag cagcccccgc atcatctgcc gccgtgtgcc gtgcgccggt
     5461 cggcctctcg ccgggccctc cgcctcccgc tccgtgccgc tcggtgttcc gcccagattg
     5521 acgaccatgg ctcgctcctc gtccggttcg gtgatccgta ccggtaaccg agcgacgggc
     5581 cccgcggtca ccgcccacgg ggccacacgg ggcaccggcc accgcgacgg agggaccggg
     5641 aacgagaccc gcgcgggcct acagtccggg gccgacgccc aggtcggccg cgggcaccgt
     5701 ctgcaccacc ggggtgagga cgcccgcctg gggcagctgg gcggccggca ggccgaagct
     5761 cccgccgtcg ggcgcgctga gcggggcgtc cagggacaga cccggcgcca gggcccgcag
     5821 ctcggtggcc ggggcctcga cggcggcgtg gtcgaacccg tccccgagga cctgcggcag
     5881 gggctggcgc aggtcgaccc ccggcagtcc gctgcgcacc ggcagctgcg gaagcgtccg
     5941 ctccggcagc agccggccct cgacgtaccg tggcccctcg ggcgcgcccg gtgtcagcac
     6001 gggcagttcg ccggccacgt cgggcagttc catgccgaga gccgtctcgg caccgcccag
     6061 cggtacgggc acggggatcg cgccggccgc tgcggcgggg gccgccgcta cgcccacggc
     6121 ggccgccaca ccggtgacga cggcggccag ggttcgtctg gtggtcgact tcatgtcagg
     6181 cacggtccct ttcatcaatg cgagaaggaa agcggaagaa cgcggcgggg gaattcgggt
     6241 cagcggccca gcgggagacc gccgagcaga ccgcccttgg agttcagctg ggtggtcgcg
     6301 cccttcacgg tgtgcagcac ggagtccttg ttctcggtgt ccagcgcttc cgactggtgc
     6361 ttgagcggca tcacgtcgat gggttccgcg gtgagcgcgt tcacggcacc gttgaggctg
     6421 gtgggcgtga gggcctcggc gtcgtaggcg aacgcgggcg cggcggagcc ggcggcgacc
     6481 agggacccgg cgacgagggc ggcggccttg agggacttca tcgtgattcc tttcttcggc
     6541 gacccggcga ccaggtgacc cggggccacc ggaggaaatg ctgacgccgt gcctaacgac
     6601 ctgcgccggg cccggaaacc ccgcggccgg aagacatatg agatctccgg agaatcgcct
     6661 ccggggcagc cccggaaaag ggaaaaggcc gttgcgccac cccgtgaggg acggcgcaac
     6721 ggcccggaac gccgtggggc gtcaggcagg gtcggccggg agcgggttcg ccggtgcggc
     6781 ggcttccgga gtggcggctt ccggggcgct cggcagggcg ggcagcccgg ccaggtctgc
     6841 ggcgggcagg ccgctgccca ccagggtggc ggcgacgacg ttgacgacgc cgttcaccac
     6901 ttcctggacg gccggggtca cctgctcggc ggtaccggag gtggcggcct ggaccagggc
     6961 gtcgatctgc ttctggagag cggcgtgcgc gtcggcggcc agggcgtccg ccgccgcggt
     7021 cccgtcgctg tcctgcgcca ccacgggcgc cggcagcgtg accggcgtct cggcctccgg
     7081 cgcggtcacg gccggcggct gggccggggt gctcgtgccg ggcgtggcgg taccgggcgt
     7141 cgtcgtgccc ggtgtggtgg ccgtgtcgtc ggcgtccgcg tccgccgcgt cggccttggc
     7201 gagggcgtcc ttcacggcgg cggagagctg gtccgcgtcc gtggcggaga gccggccgtc
     7261 gtcggccttg aggacggcgc tgagcagatc ggtgaccggc gtgagcacgc cgcccaggcc
     7321 ggccaggctg ccgacctggt cctgcagttc ctccacgtcg gggaggggtg cgcgggacgc
     7381 cgcgtgggtg cgttcccgga ccgagtcgcc atcggccgcc atcaccgccg gcccggagag
     7441 accgatcagg aggccggcgg tgagggcggt ggtggcgatt cgccgtgcgg gcagacgcat
     7501 gggggttcct ttcggtgggg cgtcggatct tgtgcccacc gtggaatcgc ccgtggcgct
     7561 ctgcaaccga tcgaccacgc cgcgcactcg cccgcggttc ccggggccgg cgtcctacct
     7621 ggtgagacgc cgtgtcaagg ccccgaggcc gtgtcgacgc ccgcaccccc cattcggggg
     7681 cggcacaccg ccgtccgtgc ggcaaggcga accgggccac ccgtgcggca acgcgaaccg
     7741 gccgcccgct cgcggaggaa ccgcggtacg gacggccggt gcagagggtt cgagccgacg
     7801 gtcagtcgtt ctcgcactcg ttgccgaacg ccgggttcag caggccgatg acgtcgatgg
     7861 tgttgccgca gacgttgacc gggatgtgga ccgggacctg cagaacgttg cccgacagca
     7921 cgcccgggga gtgggcggcg gcggcctgcg cgccggcgtc ggcggcggcg acaccggcgg
     7981 tgcccgccac cgcggcggcg gcgacggagg tcagggccag gcccttcgcg atacgcgaca
     8041 tggagaggtg ctccttgggg ttgacacaaa catccgggcg ggtgcccggc gcagtgtcaa
     8101 cgcgtggcac gcacgtgagt tgtgccgccg aaagagtgat gtcaccgggg cccggcgttc
     8161 acggatccga cacacttacc cggtttcacc gtattagtac ggatagcggc tagagttccg
     8221 caccatctga cgcatccctg ttgcaccact tgcgggactt cggccgaggg cactctccga
     8281 gaactccgaa cgtggcggtg accagccgga cttccggctc gtttccccgt aggacgcggc
     8341 gtgcgccggt gtacgccgcg tcgcggtcgg cgcctgcgat gagacgcgtt cccgcccgtc
     8401 ccgagcattt tccgtgagcg aggaaacgcg tatgcacctg ccaccgaccg agtcccggcc
     8461 acgggttctg cacatcaccc agccggtcga aggcggagtc gcccgtgtcg tggcggacct
     8521 gacgcgggct cagctcgccg ccggtctgcg cgtcgcggtc gcctgtcccg acggggacct
     8581 cgcggaccgg ctccggtcgc tgggcgccga cgtgcggcac tggccggcga cgcggtcgcc
     8641 gggtccggcc ctgttccccg aggtacggcg gctcgcccgg gtggtccggg aggtccggcc
     8701 cgacctggtg cacgcgcaca gcgcgaaggc cggtctggcg ggacggctcg ccgtacgcgg
     8761 gcgggtcccg accgtgttcc acccgcacgc ctggtcgttc gaggcggtcg acggggccgc
     8821 cgcggccctg gcgctcggct gggagcggtg gggggcgcgg tgggcggcgc ggacggtgtg
     8881 cgtcagtgag gcggagcggc tgcggggagc gcgggccggg gtgcgcgccc gctggtccgt
     8941 gatcccgaac ggcatcgacc tcgaccgctt cggtgcggac gacgtggccg agcaggcgcc
     9001 gctggtcgac gtggggacgg ccggcccgga cgccccgctg gtggtcgacg cggggacggc
     9061 cggcccggac gccccgctgg tggtctgcgt ggggcggctg tgccggcaga aggggcagga
     9121 cgtcctgctg tccgcctggg agtcggtcct cgcgcgcgtc cccggggccc ggctggtgct
     9181 ggtcggcgac ggtccggacc gggcacggct caccgagcgg gcctcggcgg gccggccggc
     9241 cccgcccggg ccgggggacg gccaccccgg ggagagcgga ccggcctccg tgctgttcac
     9301 gggtgccgtc gtggacgccg tcccctggta ccgggccgcc gacctggtcg tcctgccgtc
     9361 gcgctgggag ggcatggcgc tggccccgct ggaggcgatg gcctgtgggc ggcccgtggt
     9421 ggtgaccgac gtggacggtg cccgggagag cctgccgccc gccctcgtgc cgcactgcgt
     9481 ggtgccgccc gagaacccgg cggcgctggc cgatgccgtc gcccggctgt tgctcgacgc
     9541 tccgctgcgc acctcgctcg gccgccgggg gcgcgcgcac gtgcggtcca cgcacgacgt
     9601 acggcacacg gccgcggcgg tcgcggcgct ctacgacggc ctcctcgtcg ccgagggcgc
     9661 gacgaccggg ccgctcacgg tccacggcgg ccgaccgccg gcgcccgcgc cgcaccaccc
     9721 cgacagcaga ccgagccctc cggcgcaccc tcgtgagggc agcggcccgg cccccgcgga
     9781 acgtcccgcc tccgtccccc ggacgcccgc cgaaccccac ctccccggca cccggccggc
     9841 ccgcacggag ccccacctcc ccggcacacc ggccgagctt cacccccggg gcacccggcc
     9901 ggcacccgcc gaatcccgcc tccggggcag ccggcccgca tcccccgtgt ccgacctccc
     9961 cggcacccgg ccggcgcccg ccgcgtcctg cctccccggt gacggggccg cagccggggg
    10021 gcgggccggc gccgcgccca tcgagtacag ggagcccacc cacccgtgac cgcggaacgc
    10081 accgtcccca accccgccgg ccagccgcgc gacctcggct cctcccccgt gtcggtgctg
    10141 ccgcggcgca ccggcgcggc gggcttccgg ttccccgccg ggcggcccgc gccgcggtcc
    10201 ggctccccgc gtcggccgga ctccccgctg ccgctgctcg tcgcggacgg cgcggcggcc
    10261 ctgccgggct cgctggtcct caccggcgcc ccgcaccagc cgctgctcac ggccctcctg
    10321 gtggccgggg cgctgctgct gcgcccgcac ccgccccggc gcctgccggg tgtactggac
    10381 gacctgcccg cgctgtgcgg ccgtatcgcg gtggtctggc tggcgctggc cgcgctcgtc
    10441 gcggcgtacg ccccgccgca ggcgctgtcc gcccgcaccc tgctcctcgg cttcctcctg
    10501 cacgcggcgg ccggctgcgc gggccggg
//
