LOCUS       NZ_CP012382            21167 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Streptomyces ambofaciens ATCC 23877 chromosome, complete genome.
ACCESSION   NZ_CP012382
VERSION     NZ_CP012382.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces ambofaciens ATCC 23877
  ORGANISM  Streptomyces ambofaciens ATCC 23877
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8303940)
  AUTHORS   Thibessard,A., Haas,D., Gerbaud,C., Aigle,B., Lautru,S.,
            Pernodet,J.-L. and Leblond,P.
  TITLE     Complete genome sequence of Streptomyces ambofaciens ATCC 23877, the
            spiramycin producer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8303940)
  AUTHORS   Thibessard,A., Haas,D., Gerbaud,C., Aigle,B., Lautru,S.,
            Pernodet,J.-L. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Faculte des Sciences et Technologies,
            Universite de Lorraine, Boulevard des Aiguillettes, Vandoeuvre les
            Nancy F-54506, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC workbench v. 7.0.3
            Genome Coverage                   :: 114
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001267885.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 02:57:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,510
            CDSs (total)                      :: 7,418
            Genes (coding)                    :: 7,242
            CDSs (with protein)               :: 7,242
            Genes (RNA)                       :: 92
            rRNAs                             :: 5, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 74 of 176
            Pseudo Genes (incomplete)         :: 131 of 176
            Pseudo Genes (internal stop)      :: 12 of 176
            Pseudo Genes (multiple problems)  :: 39 of 176
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:42:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 256006
            Orig. end                         :: 277173
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012382.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21167
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..21167
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21167
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11167](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11167
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(<520..>600)
                     /locus_tag="SAM23877_RS40800"
                     /pseudo=""
     CDS             complement(<520..600)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640352.1"
                     /locus_tag="SAM23877_RS40800"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="histidine kinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRAAERTTIARELHDRVAHHVTSMVLR"
     gene            complement(<712..871)
                     /locus_tag="SAM23877_RS36995"
                     /pseudo=""
     CDS             complement(<712..871)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981454.1"
                     /locus_tag="SAM23877_RS36995"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MESFLPPGGARGGRWSDHRRVISGVLYRVRTGCRGGICRNVSGPG
                     RR"
     misc_feature    complement(857..859)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1008..2321)
                     /locus_tag="SAM23877_RS01230"
                     /old_locus_tag="SAM23877_0304"
     CDS             complement(1008..2321)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /locus_tag="SAM23877_RS01230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0304"
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="WP_079029958.1"
                     /transl_table=11
                     /translation="MKRAVAIRSAAAMMAVLALAGCGASGGAGSKDAGAEGETGAGPSA
                     TPSEEMRAVKAPKRFSTGEAIVLPESAGAGNVAIAGVQHPLPIALHKGVAYIARPDGLE
                     IAGGYFRSDPVLITPEREPLSRLDDLGPVVGGNTARRPVVTEYGGKTLALAALVGRVPG
                     SGTTKGHDALELMAADTSQGTKAWSVEIPLAEGHYSVEEGQSEVVGHSGRVVVVMGHGQ
                     LFGVDLDSRRKVWAAPGDFQEGAVLVGGTVVGLRTEKGGSDREVVGIDPATGEQRWLLP
                     GVAAERLSAAGPRTVLVKGYVADTPDDGAVLVDAVSGDQLRQFPEDTAADGTCAYDGLS
                     TTVCTDADRVAAYDAATGEQRWALPDEAGTRAAPEVTLVREGLVYGTTAENGPVVVDAT
                     TGEDAETQPGIAPYVSDGYVGIAPAEEDNAVTAYRVRS"
     gene            complement(<2411..>2749)
                     /locus_tag="SAM23877_RS01235"
                     /pseudo=""
     CDS             complement(<2411..2749)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386365.1"
                     /locus_tag="SAM23877_RS01235"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="restriction endonuclease"
                     /pseudo=""
                     /transl_table=11
                     /translation="MHHTRFEDAVRELMQRDGCRDAQRVGSRGDLGWAPT"
     misc_feature    complement(3212..3226)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.08"
                     /tool="antismash"
     gene            3217..3771
                     /locus_tag="SAM23877_RS01240"
                     /old_locus_tag="SAM23877_0305"
     CDS             3217..3771
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SAM23877_RS01240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAM23877_0305"
                     /product="hypothetical protein"
                     /protein_id="WP_053126028.1"
                     /transl_table=11
                     /translation="MRIDRLVIAVFTAVATSIAVAPAQGAEATQAALAAVNCTGTSSVT
                     YSPAITPQPTTVTATSSQDYLACVVLDGGSITFAEDSTTVTSLTRSCATLLGTTPNRQQ
                     SVEWNTGQISQAQVDVTTTQVQSALVVTSIGTVISGPFTGLNYKRVSTYANVDALLSCQ
                     SPQGLSQLGPEVSNLTITPLI"
     misc_feature    3236..3250
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.32"
                     /tool="antismash"
     gene            3828..4040
                     /locus_tag="SAM23877_RS01245"
                     /old_locus_tag="SAM23877_0306"
     CDS             3828..4040
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SAM23877_RS01245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAM23877_0306"
                     /product="hypothetical protein"
                     /protein_id="WP_053126029.1"
                     /transl_table=11
                     /translation="MSANFNTNVVGLVGVVLEVIIIGTVPSGLFQGDAVVLKQTGPAAQ
                     VLQCTLGLDTVSSVYSTVTLEITSL"
     misc_feature    complement(4349..4362)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.03"
                     /tool="antismash"
     misc_feature    complement(5266..5282)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.99"
                     /tool="antismash"
     gene            5366..5974
                     /gene="hxlB"
                     /locus_tag="SAM23877_RS01250"
                     /old_locus_tag="SAM23877_0308"
     CDS             5366..5974
                     /EC_number="5.3.1.27"
                     /GO_function="GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA]"
                     /GO_process="GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="hxlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329021.1"
                     /locus_tag="SAM23877_RS01250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0308"
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="WP_053126031.1"
                     /transl_table=11
                     /translation="MVDQQNQPAPPAPLTVGVDGPLAAARRTILREIEALLTRAREDQA
                     QELMQAVYGAQRIMVLGAGRSKLAVDAFAMRLMHLGLTAHVSTDVTCPAIAEGDLLVAC
                     SGSGHTPTVVQRTEAAKQAGARIAVVTANQESPLAALADIRVHLAEYSQDFEPDASTQF
                     VGTLFEQGALVFFDCLILALQRTQHVDPSEMYARHTNLE"
     gene            6034..6777
                     /locus_tag="SAM23877_RS01255"
                     /old_locus_tag="SAM23877_0309"
     CDS             6034..6777
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /locus_tag="SAM23877_RS01255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0309"
                     /product="triose-phosphate isomerase family protein"
                     /protein_id="WP_053126033.1"
                     /transl_table=11
                     /translation="MSIPSAPISFTSTKTQLGLTRSQDWIENLLLPNAERLAAVSFFAC
                     LPHPLLLPARQWLRGSGIGVAAQDVAAATDEVTGAVSASLLAEADCSHVMVGHAERRGT
                     FGETDQLVARKAAAASAAGLTPVVCVGETDMLNASEAATLTSAQARTVVAAVPPERPLL
                     FLYEPGWAIGGTEAADPVHAGHVLRALQDVAAGHHARFVYGGAVVPGVYTRLRNEADWD
                     GVALGRAAQDPLLLTEALDELTTPK"
     gene            <6934..>7056
                     /locus_tag="SAM23877_RS41025"
                     /pseudo=""
     CDS             6934..>7056
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037846392.1"
                     /locus_tag="SAM23877_RS41025"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAALRTSIGDCTSFSTATHLASYAGLASTTEPSGTQVDGEH"
     gene            7331..8206
                     /locus_tag="SAM23877_RS01260"
                     /old_locus_tag="SAM23877_0310"
     CDS             7331..8206
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /locus_tag="SAM23877_RS01260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0310"
                     /product="UbiA family prenyltransferase"
                     /protein_id="WP_107408634.1"
                     /transl_table=11
                     /translation="MSLLPRGSTPLDGPASPRPSALTLLAWTNVLMGLSAASATFATCQ
                     VLGEEADFIAAAFAFAIITATYTRDRLTGPDSEGAPGSREAWMARHHQAMRTWMGLCVL
                     LALVTALLRPWCALALALAWISARLYAKPWISARGRRVALRKLPGMKVPYVVAVWVTVA
                     VLPVAAGNDLLTLASTWWWALSVLLIGSVEVINNDLRDVGADRLDGTRSLPVLLGVAGA
                     KRLGYALAVLGLLVSTAVAPLVGLVWGGYAAALLACHSPARDTRLRPWIESQVAVAALA
                     ASVPADAFGF"
     gene            8689..9126
                     /locus_tag="SAM23877_RS01265"
                     /old_locus_tag="SAM23877_0311"
     CDS             8689..9126
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 4HBT_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SAM23877_RS01265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAM23877_0311"
                     /product="thioesterase family protein"
                     /protein_id="WP_053126037.1"
                     /sec_met_domain="4HBT_2 (E-value: 3.9e-06, bitscore: 26.0,
                     seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTFTTRRLIRLSDADIRGNVHNLSIIRYAEDTAVEWVCALRDEL
                     GLQTYWCAVGVQAEFTRPLPWTIPEIQLALRAEKVGRASLHLVTSISSPLGDHAQVRTR
                     VMRLDASGTRTTMTPDERAWYQRYLAQDTSHPDAARVLQPS"
     gene            10001..11167
                     /locus_tag="SAM23877_RS39995"
                     /old_locus_tag="SAM23877_0313"
     CDS             10001..11167
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SAM23877_RS39995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAM23877_0313"
                     /product="hypothetical protein"
                     /protein_id="WP_162492080.1"
                     /sec_met_domain="T2TS (E-value: 4.2e-19, bitscore: 67.1,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVSASAETPPAAARHVRCAVERAWCYLNRAMDDGAPSAQLSLDV
                     SFSRSFSGARARAVLREHLGADPGFDLADEAFTAMVALMLLPPAATAEEKSLALRLGRQ
                     IEAARWQRRYRFFLGANGFPADTDCTGLAVGALYEHGLLTPADLTAHVADLLHAAAPAD
                     SVPRPYGDDDALRARVFMVYWEDGLEPHALRRGRKHDPVACANALYTAQLSDLHAASES
                     AAAVVSTSTRYLAEHLESGRYLDGTRYYPSPDAFLYALSRLCARFPSSAQVYSAPLRRA
                     LQEHEEANSSPRNGGEPGPLALAFRTLAADNLGLTAGQDQRHALLAQTQQSDGSWPASP
                     YYRMGRFPVYFGSPYLTTAFALAALRTRQTLEAAAPHSFSGRTAAPTG"
     gene            complement(11210..12466)
                     /locus_tag="SAM23877_RS01280"
                     /old_locus_tag="SAM23877_0314"
     CDS             complement(11210..12466)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998206.1"
                     /locus_tag="SAM23877_RS01280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0314"
                     /product="MFS transporter"
                     /protein_id="WP_053142018.1"
                     /transl_table=11
                     /translation="MPETQKRILVVLLASQVLSGVGLAAGVTVAALLAQDMLHSTTLAG
                     LPSALLTAGSALAAVTIGRISQTRGRRPGLAAGYLAGAVGGAGVVVAAALDNPGLLFIS
                     LFLYGAGTAANLQARYAGADLAAPRHRARALSTVLVATTLGGVVGPNLTAPAGHLAAAL
                     ALPPLSGLFLISSAAFALAALTLLVWLRPDPLLLARSLPAVPEAPEHTGTGPATDIPQP
                     AAHRRPGLPLGVLVMVLSQLVMVAIMTMTPVHMHDHGHSTAASGLVIALHVGAMYLPSP
                     LTGALTDRYGPTATAAASGLTLLAAGVLAAAAPADSVLLLTLALVLLGIGWNFGLVSGT
                     TIITNAVPLATRAKTQGMVDVAIAIAGATGGMTSGIVVAAASYPLLALAGAAFALALLP
                     AVVVTAQKGSPRTPSSERR"
     gene            complement(12657..13757)
                     /locus_tag="SAM23877_RS01285"
                     /old_locus_tag="SAM23877_0315"
     CDS             complement(12657..13757)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1251: luciferase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382271.1"
                     /locus_tag="SAM23877_RS01285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0315"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_053126041.1"
                     /transl_table=11
                     /translation="MNNPIKGTARGSAPAPLSVLDTALAGRGISAADALSGSIALAELA
                     ERRGFTRYWVTEHHSMPGVSSSSPAVLLARLTAHTTRLRLGAGGVMLPNHPPLIVAEQY
                     GLLQALAPDRIDVGLGRAPGTNQATAAALRRGRSDNDDFPRQVVELLHFLDDDFPEEHP
                     YRERVYAVPGPGQDKENGLSQTAGRPSVWLLGSSGYSAHLAAGLGLPFAFAAHFSPDHV
                     AAALQLYRDRFTPSAVLTEPYALASFAVMAADDEHEALRQTHSYAHSMMRMLHGKSYLV
                     PTPDEAASHPYTASEQQVLDHWTNSVLHGTPDQVTAHLNRLHEAARPDEIMIASVGHAP
                     QALLHSTELIADAYTLPTSPVPASAP"
     gene            complement(13859..14611)
                     /locus_tag="SAM23877_RS01290"
                     /old_locus_tag="SAM23877_0316"
     CDS             complement(13859..14611)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172861.1"
                     /locus_tag="SAM23877_RS01290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0316"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_053126043.1"
                     /transl_table=11
                     /translation="MSNEQVPIRVFGGPTALIEYGGLRFLTDPTFDAPGTYPSGLAKTA
                     PASAAPADLGRIDAVLLSHDEHDDNLDASGRALLADVPLTLTTPSGAGRLGGTARPLKD
                     WESIELDRPGGGTITVTGVPALHGPGAREDLEPVVGEVVGFVLSGDGLPTVYVSGDNAQ
                     LSLVQEIADRFGPVDTAVLFAGAVRTPVLDRALLTLDSAQAAEAARILGARRIVPVHFD
                     SWAHFTEGREQLVDAFTTAGLIDRVELA"
     gene            14713..15330
                     /locus_tag="SAM23877_RS01295"
                     /old_locus_tag="SAM23877_0317"
     CDS             14713..15330
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677186.1"
                     /locus_tag="SAM23877_RS01295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0317"
                     /product="ABATE domain-containing protein"
                     /protein_id="WP_244902887.1"
                     /transl_table=11
                     /translation="MSEIDTAAPAETAAPGLPQAPGAGQYPALDLANSAIALPGGQFLD
                     ALGTPAGANQWLADHNLAPADAGLQEICAARVRSLREQVRALLAAQVGGHPAPATALAA
                     VNDALTRVPTASPLGWDAVRGMHRTAPHPIDQIVDQALGILAADAADLLTGPDAERLTA
                     CPSHPCNRYLLRAGRRHWCSVRCGDRARAARAYARRTQTTAD"
     gene            15474..16079
                     /locus_tag="SAM23877_RS01300"
                     /old_locus_tag="SAM23877_0318"
     CDS             15474..16079
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038531.1"
                     /locus_tag="SAM23877_RS01300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0318"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_053126047.1"
                     /transl_table=11
                     /translation="MTATFTLLTTGYGRLDAPNDGRDGREKPWYVASTVGLLCDGDTIV
                     VVDPGMVEDRKLILDPLAAAGITPEQVTDVVFSHHHPDHTLNAALFPNARAHDVWAAYH
                     NHLWYDRPADGCQLSPSIRLIETPGHTPQDISTVAETAEGTVVLTHAWWSVHGPAEDPT
                     SDDPAGLSASRRKILALKPALIVPGHGDPFVPDENTPI"
     gene            complement(16726..18192)
                     /locus_tag="SAM23877_RS01305"
                     /old_locus_tag="SAM23877_0320"
     CDS             complement(16726..18192)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114909.1"
                     /locus_tag="SAM23877_RS01305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0320"
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="WP_053126049.1"
                     /transl_table=11
                     /translation="MSTSPADPHLPTVHLRPPRNWINDPNGLVFHDGHYHVFFQYNPHG
                     PQHANMHWGHYRSPDLITWEPLPVALTPTPGGHDADGCYSGNAVSEGNRMVAFYSAHRN
                     DRWWQPITTAESSDNGRTWAKRPHLLIPEPPAGTTMYRDPYVWRQDERWRMLVGSALDD
                     GRAAAQLYESDDLEHWTYRGPFHTSDAATGTGPIGWECPQYATFGGQGVLILSDWTPQG
                     GPSHTSVIIGQEKDGRFTAATALVPLDHGPDFYAPALLKAPGEDRWLMWGWAWEARDDA
                     WAHEAGWAGVLTLPREVSLTADGTVAQRPARELLALRGPRVLYGTGHVSRTEPVELGQV
                     SHTFDLTATLVPDTTGTSGLRLITSTDGSEYLDISLDPTAGRLAVDRTHASPDTRARGG
                     AYTAPCPAAARLHTPVELRVIVDRSIAEVYLADEQVLTLRFYPSTDGPWRLQARTTGTG
                     RSEFAVAAWNLTPQDIRQEAEPAPRTAQRA"
     gene            complement(18269..19141)
                     /locus_tag="SAM23877_RS01310"
                     /old_locus_tag="SAM23877_0321"
     CDS             complement(18269..19141)
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1069: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996828.1"
                     /locus_tag="SAM23877_RS01310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0321"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_053126051.1"
                     /transl_table=11
                     /translation="MTSLLKRLSGHAGRVVLALVFALPLLFMLVSSFKPDDQIFGDLGS
                     LRAFLPVGTLSLDNYTDMFERVPAARFLLNSVLISSVTVVLGIIVNSLAAFALSRMRWP
                     GRKLVLTVIIATLIVPFETFALPLVWWVNQLPLLRVNGFHLAWTTGWMDTYQVQVLPFV
                     ANAFSIFFFHQFFQSIPKELDEAAIMDGAGWFTIYRRIVMPLSGPAVATVAILTFLPAW
                     NSYLWPLMVVQSEELRPVMVGISYFFQLNVGWGQIMAYSSMITVPILALFVAFQRSFVN
                     SIASTGVKG"
     gene            complement(19143..20078)
                     /locus_tag="SAM23877_RS01315"
                     /old_locus_tag="SAM23877_0322"
     CDS             complement(19143..20078)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996827.1"
                     /locus_tag="SAM23877_RS01315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAM23877_0322"
                     /product="sugar ABC transporter permease"
                     /protein_id="WP_053126053.1"
                     /transl_table=11
                     /translation="MTTRTAAPERLRPVRRTRTTNTRRRESLTALGMATPAVVLLIVFL
                     VVPVALAFALAFTDARLISPTPARFVGLRNFTRLFEDPVFYKSLRNTAYFAAVVVPLQA
                     GLALVLALLVNAKVRGVNFFRTVYFLPVVTSMVVVSLLWTFLYRKDGLVNHVISTLTLG
                     HFQGPDWLGDPATAMPAIILMSVWQGVGFHMIIWLAGLQTIPAELYEAAEIDGATPWHR
                     FCHVTWPGLRATRTFVLVTITIAAFSLFTQIRVMTQGGPLDSTTTVVYQAVRTGYDQQQ
                     TAYAAAISLIFFVLVLTVSLVQRFLTREKD"
     misc_feature    complement(20199..20213)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
ORIGIN
        1 agtgcgaccg aggacggccg caccgccctc ctggagtggc tccacgccct cgtccactac
       61 gccgtttcag cccgcggatt cgccaacacc ctcagctacg agcctctcac cgaggaatct
      121 tccccgcact cctgtggcaa cagaatcgca gcggccggaa ctcccctgct ccagcgggcc
      181 attcgcgaca aggcggttgc accgcacgtc acctttcacg acctgctgac cctcgccgtc
      241 ggcatcgccc tggccaccga gcaccacaag gaccccgagg tccaggccga ccgcctcttc
      301 cgcctcaccg tggaagggct cagccctgag cacggcaccg ctgaggtatc cgagaactca
      361 gggtcttcgg tactgggagg cggttaagga tgtccctgct gaagggggcc tctccgttgg
      421 cgatccggcg tacggcgtcc aggagctcgc ctggtggggt gtccttgacg aggaaaccgc
      481 aggctccggc cgcgagcacc ggatacgtgc gacaccaacg cgcagcacca tggaggtgac
      541 gtggtgggcc acgcggtcgt gcaactcgcg tgcgatggtg gtgcgctccg ctgcccgggc
      601 ggccagcagc tggcgttcgg cacgaagttc ctgctgctcc gtccgctgac gggcgtgcac
      661 cgcggctcgc gggtcacgcg cacgtacgcg cccaggagca gtggcaagcc tgcgacgatg
      721 cccatcttca ccgtctccca gggcccgaga cgttccggca gatcccgcca cggcaccccg
      781 tccgcacccg gtagagcact ccgctgatca cccgacggtg atcgctccac cgacctcccc
      841 gcgcaccacc aggaggtaag aacgactcca gccgagccca tgtcgcggag gacgtgtccg
      901 cggcctcccg gacgacgcgg gggcccgtcc cacgtcacgc tggagggact ggacggctga
      961 gcgcgcgtgc gggtgcgacg gtccgccaca cccgcacgcg cgccggttca gctcctgacc
     1021 cggtaggccg tcaccgcgtt gtcctcctcc gcgggggcga tgccgacgta tccgtcgctg
     1081 acgtacggcg cgatccccgg ctgcgtctcc gcgtcctcac cggtggtggc gtccacgacc
     1141 accgggccgt tctcggcggt ggtgccgtag acgaggccct cacgcaccag ggtcacctcg
     1201 ggggcggcgc gggtcccggc ttcgtcgggc agtgcccacc gctgctcacc ggtcgccgcg
     1261 tcgtaggcgg cgacccggtc ggcgtccgtg cagacggtgg tcgaaaggcc gtcgtaggca
     1321 cacgtgccgt ccgccgccgt gtcctcgggg aactgtcgta gctggtcgcc ggagacagcg
     1381 tcgaccagga cggcaccgtc gtccggggtg tcggccacgt aacccttcac caggacggtc
     1441 ctgggacccg ccgcggagag cctctcggcg gccacgccgg gcaggagcca cctctgctcg
     1501 ccggtggccg ggtcgatgcc caccacctcc cggtccgagc cgcccttctc cgtgcgcagc
     1561 cccacgacgg tgccgcccac caggacggca ccctcctgga agtcgcccgg tgccgcccac
     1621 accttgcggc ggctgtcgag atcgacgccg aagagctgcc catggcccat gaccacgacg
     1681 acgcggccgc tgtgcccgac gacctcgctc tgcccctcct ccacggagta gtggccttcg
     1741 gccagcggaa tctccaccga ccacgccttg gtgccctgcg aggtgtccgc ggccatcagt
     1801 tccagggcgt cgtgcccctt cgtcgtgccc gatcccggca cccgcccgac gagcgcggcc
     1861 agcgccagcg tcttgccgcc gtactccgtg acgaccggcc tgcgggcggt gttgccgccc
     1921 acgaccggcc cgaggtcgtc gagcctggag agcggttcac gctcgggtgt gatcagtacc
     1981 gggtcgctgc ggaagtaccc gcccgcgatc tccagcccgt ccggtctcgc tatgtacgcg
     2041 acgcccttgt gcagggcgat gggcaacggg tgctgcacgc cggcgatcgc gacgttgccc
     2101 gcccccgccg actcgggaag gacgatggcc tcgcccgtcg agaagcgctt cggcgccttg
     2161 acggcgcgca tctcctcgga gggggtggcg gaaggcccgg ccccggtctc accctccgcc
     2221 cccgcatcct tcgagcccgc accaccggac gctccgcacc cggccagcgc gagcaccgcc
     2281 atcatcgcgg cggcactcct tatggccact gcgcgcttca tcaagttccc ccttcgccga
     2341 tcatgacggt ggtgaggata gccaacgggc gggcaccgca ccgagcctcg ccggaaacgg
     2401 ccggcccggc cagggcctgg cggtcgacgt cgtgcagccg ctgctgcctg gcgacggtga
     2461 cggcggggtc gttcacccga ccgttggtgg tgatcactgc gacgtcggcg ccgtgtacct
     2521 ggcgggcggt tgccgttgag gacctggagg tccggcgtgc ccaccggtga gccctgggcg
     2581 ccgttgcggc ggtacttgtg ctggatgacc cagcgccgcc cgtgggggtc gatggctttc
     2641 acgtcggcgc ccagcccagg tcgccacggc tgccgacccg ctgggcgtct cggcagccgt
     2701 cgcgctgcat gagttctcgt acggcgtcct cgaatcgggt gtggtgcagg gtccagcgcc
     2761 ggcaggccgc acccgcaccc gcacccgcac ccgcaccgat gaacctctcg ggcacgaacg
     2821 ccgaagacct cgcgctgtac cggtaggagt tctgtggccg tctgccggag gcgagtacgt
     2881 tcaggccggc acgcccttgc tgcccgtctg ggcgcgtgct gaggggtccg tggcggtggc
     2941 ggtggcgtct gtcatcgctg gctcggaagc tccgaggggc ggacggtttg cgcgctggct
     3001 tcacccgccg tgtgtacggc gcgtaggggg tacgcggaca cgcactatgg ttcacatccc
     3061 ctcgatcggg ctatggccct gtgtcatgcc ggcgcggaat gctcacttct cggacacggg
     3121 ctgttggcgc caagcggagc cgcttgcagg ctgcagccag tgcccttccc tcttcgccca
     3181 tgcaggggcg gcacccttct cggctcagga gtgaacatga gaatcgaccg cctcgtgata
     3241 gcagttttca ccgccgtggc gacatccatc gccgtggccc cagcgcaggg ggccgaagct
     3301 acgcaggctg ccctcgccgc ggtcaactgc accggcacgt cctcggtcac ctactcaccg
     3361 gcgatcaccc cacagccgac aacggtcacc gccacctcga gccaggacta cctggcatgc
     3421 gtcgtcctgg atggaggaag catcaccttc gccgaggatt ccacgaccgt tacctccctc
     3481 acccgttcct gtgccaccct gctgggaacc acgccgaacc ggcagcagtc cgtcgagtgg
     3541 aacaccggcc agatctcaca ggcccaggtc gacgtcacca ccacccaggt ccagtcagcg
     3601 ctcgtcgtga ccagcatcgg aaccgtgatc tccggtccct tcaccggact gaactacaag
     3661 cgtgtgtcca cctacgccaa tgtcgacgcc ctcctctcgt gccagagccc tcaagggttg
     3721 tcccaactcg gccccgaggt ctcgaacctg accatcacgc ctctcatcta ggcggcgcgg
     3781 cgggagcggc gcctgacgct gctacgggct ggaggccggc cgccgccgtg tcggcgaact
     3841 tcaacaccaa cgtggtgggc ctggtgggcg tggtgctgga ggtaatcatc atcggtaccg
     3901 tcccgtccgg gctgttccag ggcgatgccg tggtactgaa acagaccggt cccgcggccc
     3961 aggttctgca atgcaccttg ggcctggaca cggtctccag cgtctacagc acagtgaccc
     4021 tggagatcac ttcgctctga cggctctgtc gccgtgcacc accggatcgg acccgccgga
     4081 ggcgctggct cgggtcacgt tcgaacggga gcccggtacc cccggaagcc gggatcccgc
     4141 ccgacgtaca ccccagggcc ggtcggggag tgccagcacg cctcagcggc gtggaccgct
     4201 tctcactcgg tggcgactag gagaacggtc gccagcgaac agagacgcta cgcccagggg
     4261 cccgttctcg ccggagttcc cccgcgcgcg ggcaccacgg gcagcagcag gcgcatgtct
     4321 tgtgggatac ggggcccggc gggtgcgccg aacaggcgat tggggacgag cctggggcat
     4381 ggctggttcg tccccctggt gcttgatccg aacacccacc gggtggattc tcagtaagtg
     4441 ttcaactacg tcgggcagtc agccgctccg tagaggcgat ccgcgcggtc cgcgccccac
     4501 tacggcgccc agccatgcga cctgccgcgc aaggccgccc gagcccgccg ggcactcggg
     4561 gccgccggga acgaggatcg gcggacgccg ggggccgacg aacgaccagc cgcctcggcc
     4621 agacgcgtga ggaaccccgc atgaccgact tcaccgcacc caccgatgtg ccgtccgccg
     4681 accgggacgc cgtcctgcgg atcctgcaga ccacccgctc cgtgcgccgc cgcctcgaca
     4741 ccgaccgtcc cgtagacctc cggctcgtgc acgcggcact cgaagcggcc ctgcaagcct
     4801 ccaacgggtc gaacgagcag ccatggcact ggatcgtcgt gaccgacccc gcccgccgta
     4861 ccggcgctgg cccgctgcta ccgcgatgcc gcccggccct atctcgcggc gatgacacag
     4921 cgggtggagg acggcgtcgt cggccgtggc gtgctgaacg cgtccctgcg gctgaccgag
     4981 gtactcgacc gggtgccggt gatgatcgtt ccctgcctcg cgtccacacc cgccgaccac
     5041 gtcgacctct tccgccggct cggttacgcg ccgtccgcgg agcacgccgc cctctccgtc
     5101 tactacgggt ccgtctggcc tgccgtctgg agcctcatgc tcgccctgcg cgcccattcg
     5161 ctggcaagcg cggtcaccgc cctgcatctg acccgaccaa gacgacgcaa acgcgatcct
     5221 caacctgccg gaaggcgtca cccaggccgc gctgctcgca gtcgcacacc ccaccgggga
     5281 atcgttccgc ccggctcctc gccgctccct cgacaccgtc ctccaccacg acatctggta
     5341 gcccggtaca tgaaaggacc acgggatggt cgaccagcag aaccagcccg cgcctcccgc
     5401 tccgctgaca gtcggagtgg acggaccact cgccgcagcc cgccgcacga tcctgcggga
     5461 gatcgaagcc ctgctcacac gcgcgcgcga ggaccaggcc caagagctca tgcaggcggt
     5521 gtacggcgct cagaggatca tggtgctggg ggcgggccgc tccaaactcg ccgtcgacgc
     5581 cttcgccatg cgcctgatgc acctcgggct gaccgcccac gtgtccaccg atgtcacctg
     5641 cccggccatc gccgagggcg acctcttggt ggcgtgcagc ggatcgggtc acacccccac
     5701 cgtggtgcaa cggaccgagg cggccaagca ggccggcgcc cggatagccg tcgtgaccgc
     5761 gaaccaggag tccccgctcg ccgcactggc cgacatccgt gtccatctcg ccgagtacag
     5821 tcaggacttc gagcccgacg cgtcgaccca gttcgtcggc accctgttcg agcagggcgc
     5881 tctggtcttc ttcgactgtc tgatcctcgc actccagcgc actcagcatg tcgacccctc
     5941 cgagatgtac gcccggcaca ccaaccttga gtgacgaccg tgccgcagcc gattcagcac
     6001 cggacatccg cccccacggg aaagcagccc gcgatgtcga tcccttccgc gcccatcagc
     6061 ttcaccagta ccaagaccca gctcggcctg acccgttcgc aggactggat cgagaacctg
     6121 ctcctgccga acgccgagcg cctggccgct gtctccttct tcgcctgcct gccccacccc
     6181 ctgctgctcc cggcccggca gtggctgcgc ggcagcggca tcggcgtcgc cgcccaggac
     6241 gtggcggcgg ccaccgacga ggtgacgggc gcggtcagcg cctcgctgct cgccgaggcc
     6301 gactgttccc acgtcatggt cgggcacgcc gaacgtcgtg gcaccttcgg tgagaccgac
     6361 caactcgtcg cccgcaaggc cgccgccgcg agcgcggccg gtctcacccc ggtggtgtgc
     6421 gtcggtgaga ccgacatgct caacgcgtcc gaagcggcga cactgacctc cgcccaggca
     6481 cggacggtcg tggccgccgt cccaccggag cgacccctgc tcttcctgta cgagccgggt
     6541 tgggccatcg gcggcaccga ggccgccgac ccggtccacg ccggccacgt actgcgcgcg
     6601 ctacaggacg tcgccgccgg ccaccacgcc cgcttcgtct atggaggggc ggtcgtgccc
     6661 ggcgtctaca cccggctgcg caatgaagcg gactgggacg gcgtggccct gggccgcgct
     6721 gcccaggacc cgctgctgct gaccgaggcc ctggacgaac tcaccactcc caagtgagtt
     6781 cgtccagcgg caactccagc cggcgcgctc gctttcgctc gggagcgagg acctacttcg
     6841 acggaaccgg cgccatggcc ccgccctcgt actcaccgcg cacgtcgccc tcgggctcgt
     6901 cctcaacttc tcaggtcttg agtcgatgcc ggggccgcag ccctgaggac cagcatcggc
     6961 gactgcacca gcttctccac cgccacccac ctcgcctcat acgccggcct ggcctcgacg
     7021 acggagccgt cgggaaccca ggtcgacggc gaacacgtcg cccgagcccc ggtcccccgc
     7081 cgtcactctc accgcatgac caacaccgac agcgcgacat tgaagaacgg tacagaggcc
     7141 ttcgatcgag tgatgacaga aatgtgcgaa tgcccttaaa tccccccata aggcgcgagg
     7201 gcagcggagc gagtggggag aattgacctt ccgaaatgcc tgacaagcct ggcttgcatc
     7261 tcacaggcat gcccttctgc tcttcgcttc gcagtcgccg aattttggcc tgcacaggac
     7321 ggatgcactc atgtcgcttc ttccccgcgg cagcacgccg ctggacgggc cggccagccc
     7381 acggccctcc gcgctgacgc tactggcatg gaccaatgtt ctgatggggc tgtccgccgc
     7441 ctcagcgacg ttcgcgacct gtcaggtcct aggagaggaa gcggacttca tcgcagccgc
     7501 cttcgccttc gccatcatca ccgctaccta cacgcgcgac cggctgaccg gacccgacag
     7561 cgagggcgcc ccgggaagcc gcgaagcatg gatggcccgc catcaccagg ccatgcggac
     7621 atggatgggc ctgtgtgtac tgcttgccct cgtcacagcc ttgctgcggc catggtgtgc
     7681 gctcgcactg gccctggcct ggatctccgc acggctgtac gccaagccat ggatctccgc
     7741 ccgcggccga cgggtggccc tgcggaagct tcccgggatg aaggtccctt acgtcgtcgc
     7801 cgtgtgggtg acggtggcgg tcctgccggt cgcggccggc aacgacctgc tgacgctggc
     7861 gtcgacctgg tggtgggccc tgagtgtgct gctgatcggg tccgttgagg tcatcaacaa
     7921 cgacctgcgc gacgtcggcg cggaccgcct ggacggcacc cggagcctgc ccgtactcct
     7981 gggggtggcc ggagccaaac ggctcggcta cgcactcgca gtactgggcc tgttggtctc
     8041 cacagccgtg gcaccgctgg tgggactggt gtggggcggg tacgccgcgg ccctgctcgc
     8101 ctgccacagc ccggcgcgag acacccgact gcgcccctgg atcgagtccc aggtcgctgt
     8161 tgccgcactg gccgcgtccg tccctgccga cgctttcggc ttctgacggc aagagaccat
     8221 cactcaaccg ccctccaggt cagggcgggt gccctggagc ggctcggcag ttctggttct
     8281 gcgcaccgcg gcggatgatt cggagcgccg gccgatcccc gtgccgccct cgccggtcct
     8341 gcctgcgcgg agtgggcctg aaccgcccgc gtgttgggtg cggcgcggta gggcggttcc
     8401 agcgtgtggt ggtgggtgcg cattcacctc gggcggtgtg cgccgcgcgc gctcgctcga
     8461 tctgagtatg ggagcggtcc gggagaaatc gagagcaaat atctgcattg cccgtggtac
     8521 acctttcctg ttgattaagc atggcgctgc cgcgcgggtc gggggctgat ctgcggccga
     8581 tcccgactcg ggcgccgctt cccgtttccg cgaggtgccc agggctcgcc tgcccgagac
     8641 ctctgcgtca tcggcagacc ggtagcgcct cccggaacaa ggacattgat gctcaccttc
     8701 accacgcggc ggctcatcag gctgagcgat gccgacatcc gcggcaacgt gcacaacctg
     8761 tcgatcatcc gctacgccga ggacaccgcg gtcgaatggg tgtgcgcgct gcgcgacgaa
     8821 ctgggtctgc agacctactg gtgtgccgtg ggagtccagg cggaattcac ccggccgctg
     8881 ccctggacca tcccggagat ccagctggca ctacgggcgg agaaggtcgg acgggccagc
     8941 ctgcacctgg tcacgtcgat ctcgtcgcct ctgggtgacc atgcccaggt ccgcacccgt
     9001 gtcatgcgcc tcgacgcctc cggcaccagg accaccatga ccccggatga acgggcctgg
     9061 taccagcggt atctggcgca ggacacctca cacccggacg ctgcgcgagt actgcaaccc
     9121 agctgaggaa caccgaaggg ccttccgccg gctgctctgc ccgtgccgga acgccgccgg
     9181 agggcccgat atgccgcagg acccgccgct cgccccgctt tccgccccag acttcgggag
     9241 agaccttgca cggacacgcc acagcggacg gtgggctgcg gcccgctgtg agcgcccaac
     9301 acgaagtcgc cgggcagctc ctgcacatcc gcctcgccac cgccggcgac accacccccg
     9361 tcgaggagtt acacacccgc tgtgcgcccc gttcgctcca ccagcgtctt ctggcgccgg
     9421 tgccccgcgt ctcggcccgc gccctggaac agctcctcga cccccacagc accagcccgc
     9481 cccgggccgc tgctcgacga caccacctct cccccgcgtc cggcgggaga cggcgtcgac
     9541 gtgtggggcc agccgcggcg gcggaacagc ccgcggacct ttcccggatc ctcgtggtcg
     9601 tggtcgcgct ccggccccgg tggcgcggcg gcctggagcc gggcggctcc ggcagccgct
     9661 tcctcacggg cgacgacgtc ggcgtggcaa ctgccgcaca ccgacaggcc cccggcgctc
     9721 caagccgtcc ggtgcgcggt tgtctcctcc cagcgctgct cggtgaacct ctgcccgcac
     9781 cgcgcgcaca cgggctctgc gcctcccgct cagctacacg gcgccgtacg tcttccgcct
     9841 cggcgcgggc gtacgggcgg cggcagagag cgtcgccgtc ggagttgtcg agcgcgtcgg
     9901 tcagtggctg tccgtccgcg cggcccaggc gccgccacgc cgccgcgtcc gcgccgtgct
     9961 cccggagctg tcccagggtt gtcaggagga tcgaggcacg gtgacggttt cggcttctgc
    10021 ggagacgccg ccggctgcgg cgcggcatgt gcggtgcgcc gtcgagcgcg cctggtgcta
    10081 cttgaaccgg gccatggacg atggggcccc gtcggcacag ctgtcccttg acgtctcctt
    10141 cagccgcagc ttctccggcg ctcgggcacg tgcggtactt cgcgagcatc tgggtgcgga
    10201 tcctggattc gatctggccg acgaggcatt cacggccatg gtcgccttga tgctcctgcc
    10261 tcccgccgcc accgcggagg agaagagtct ggccctgcga ctgggccggc agatcgaggc
    10321 ggcccgctgg cagcgccggt atcgcttctt cctcggagcc aacggctttc ccgccgacac
    10381 cgactgcaca ggcctggccg taggtgctct gtacgagcac ggcttgctca ctcctgcgga
    10441 cctcacagcg cacgtggccg acctcctaca cgccgcagca cccgctgaca gcgttccccg
    10501 cccatacggc gacgatgacg cactccgtgc gcgagtcttc atggtctatt gggaagacgg
    10561 cctggaaccc catgccctgc gccgcggacg caagcacgac ccggtggcct gtgccaacgc
    10621 tctctacact gctcagctca gcgacctcca cgccgctagc gagagcgcgg cggcggttgt
    10681 cagcacctcg acgcgatacc tggccgagca ccttgagtcc gggcgatacc tggacggcac
    10741 ccgctactac ccgtctcccg acgcgttcct ctacgcgctc tcccgcctgt gcgccagatt
    10801 cccctccagc gcccaggtct actccgcccc cttgcgccgc gccctccaag aacacgagga
    10861 ggcgaactcc tcaccgcgca atggcggcga gcccggcccc ctggccctcg ccttccggac
    10921 actggcagcc gacaatctcg gactcaccgc tggccaggac cagcggcacg ccctgcttgc
    10981 ccagacccag cagtccgacg gttcttggcc ggccagcccg tactaccgga tggggcggtt
    11041 ccccgtgtac ttcggctcgc cctacctcac cactgccttc gccctcgcgg cactgcgaac
    11101 ccgccagaca ctcgaggccg ccgcaccgca cagcttctcc ggtcggaccg ctgccccgac
    11161 cggctagcac ttgtccgccc gatcatatgg ctcaaggttc ggtgctgctc tatcggcgtt
    11221 ccgaggaagg tgtgcgaggc gagcctttct gggcggtgac gacaactgcg gggaggagag
    11281 cgagggcgaa ggcggctccg gcgagggcga ggagagggta gctggctgct gcgacgacga
    11341 tccctgaggt catgccgccg gtggctccgg cgatggcgat ggcgacgtcg accatgcctt
    11401 gggtcttggc tcgcgtggcc agggggacgg cgttggtgat gatggtggtg ccgctgacga
    11461 ggccgaagtt ccagccgatg ccgagcagga cgagagcgag ggtgagcaga aggacggagt
    11521 cggcgggcgc ggcggcggcg aggacgccgg cggccagcag ggtcaggccg gaagccgcgg
    11581 ccgtcgcggt ggggccgtag cggtcggtga gtgctccggt caggggtgag ggaaggtaca
    11641 tggcgccgac gtgcagggct atgacgaggc cggaggcggc ggtgctgtgg ccgtggtcgt
    11701 gcatgtgcac gggggtcatc gtcatgatcg cgaccatgac gagctggctg aggaccatca
    11761 ccaggacccc cagcggcagg cccgggcggc ggtgtgcggc gggctgcggg atgtcggtcg
    11821 ccggtcctgt gccggtgtgc tctggtgcct ccggcacggc ggggagggag cgggcgagca
    11881 ggagggggtc gggacgcagc cagaccagca gagtcagggc ggccagggcg aacgcggcgc
    11941 tggagatgag gaacagaccg ctcagcgggg gcaatgcgag ggcggcggcg aggtgtccgg
    12001 cgggagcggt gaggttgggg ccgacgacac cgcccagggt ggtggcgacc aggacggtgg
    12061 acagcgctcg ggcgcgatga cgcggcgcag cgaggtcggc tcccgcgtag cgggcctgaa
    12121 gattggcagc ggtgccggcg ccgtagagga agagggagat gaacagcagg ccggggttgt
    12181 cgagggcggc agcgacgacg actccggcac cgccgacggc gcctgcgagg tagccggctg
    12241 ccaggccggg gcggcgcccg cgggtctgcg atatgcggcc gatggtgacg gcggcgaggg
    12301 cggagcccgc ggtgagcagg gcgctgggca gcccggccag ggtggtggag tggagcatgt
    12361 cctgagccag cagggcggca acggtgacac cggccgcgag ccccacgccg ctgaggacct
    12421 gggacgccag caggacgacc aggatgcgct tttgcgtctc aggcagagaa gtgccaggag
    12481 tggccgatgg ggtgtctgtc ggcccgttgg ctgacgtggt cgcggtgtgg tgcatggtgt
    12541 ctccgttacg agcccagggg tggtgggcga ggtctgctgc gtgtgacgtg ctacctggcc
    12601 gacgctgcac ggcgggcggt ccgcgcgaac tgtcccgggg gcgcggcggg gccgtgtcag
    12661 ggagcggacg cggggaccgg tgaggtcggc agggtgtagg cgtcggcgat gagttcggtc
    12721 gagtgcagga gcgcctgggg ggcgtggccc acgctggcga tcatgatctc gtcagggcgg
    12781 gctgcctcgt gcaggcggtt gaggtgagcg gtgacctggt cgggtgtgcc gtgcaggacg
    12841 ctgttggtcc agtggtcgag gacctgctgc tcggaggcgg tgtaggggtg ggaggcggcc
    12901 tcgtcggggg tgggtacgag gtaggacttg ccgtggagca tgcgcatcat gctgtgggcg
    12961 tagctgtgtg tctggcgcag ggcctcgtgc tcgtcgtcgg ccgccatgac ggcgaagctg
    13021 gccagtgcgt acggctcggt gagtacggcc gagggggtga accggtcgcg gtagagctgg
    13081 agggcggcgg cgacgtgatc gggagagaag tgcgcggcga aggcgaaggg caggcccagg
    13141 ccggcggcga ggtgggcgga gtatccggag gatcccagga gccagaccga gggtcggccg
    13201 gccgtctgcg aaagaccgtt ctccttgtcc tgtccggggc cgggcacggc gtagacgcgc
    13261 tcgcggtagg ggtgttcttc ggggaagtcg tcgtcgagga agtgcagcag ttcgacgacc
    13321 tgccgaggga agtcgtcgtt gtccgagcgg cctcggcgca gggcggcggc ggtggcctgg
    13381 ttggtacccg gggcgcggcc cagaccgacg tcgatccggt ccggggcgag ggcttggagc
    13441 agtccgtact gttcggcgac gatcagcggg gggtggttgg gcagcatcac tccgcccgcc
    13501 ccgagccgca gccgtgtggt gtgggcggtc agccgggcca gcaggacggc ggggctggag
    13561 gaggagacgc cgggcatgga gtggtgttcg gtcacccagt agcgggtgaa gccgcgcctc
    13621 tcggcgagtt cggccagggc gatgctgccg gacagggcgt cggcggcgct gatgccgcgg
    13681 ccggccaggg cggtgtcgag gacggacagc ggcgcgggag cggatccgcg ggctgtgccc
    13741 ttgatcgggt tgttcacggg gattccttct ggggtgcgcc ggcccggcgc gagtggctga
    13801 gccgggaagg gccgctgcgg tgagctcgtg atgtaccgca gcggccgggc gttggaggtc
    13861 aggccagttc cacgcggtca atcagtccgg cggtggtgaa ggcgtccacg agctgctcgc
    13921 ggccctcggt gaagtgggcc cagctgtcga agtggaccgg cacgatccgg cgggcgccca
    13981 ggatgcgggc ggcctcggcg gcctgagcgc tgtcgagggt gagcagcgca cggtccagga
    14041 cgggggtgcg gaccgcgccg gcgaacagca cagcggtgtc caccgggccg aaacgatcgg
    14101 cgatctcctg gaccagggag agctgggcgt tgtcgccgct gacgtagacg gtgggcaggc
    14161 cgtccccgct caggacgaat ccgacgacct cgccgacgac gggttcgagg tcctcgcgtg
    14221 ccccgggccc gtgcagggcc gggacgccgg tgacagtgat cgtgccgcca ccgggacggt
    14281 cgagctcgat ggactcccag tccttcaggg ggcgtgcggt gccgcccaac ctgcccgcgc
    14341 cactgggcgt ggtcagggtc agggggacgt cggcgagcag ggcccggccc gaggcgtcga
    14401 ggttgtcgtc gtgctcgtcg tgcgacagca gcaccgcatc gatacggccc agatcagcgg
    14461 gcgcggcgga ggcgggcgcg gtcttggcga gccccgacgg gtaggtgccg ggggcatcga
    14521 acgtcgggtc ggtcaggaat cgcaacccgc cgtactcgat gagcgcggtc ggaccgccga
    14581 agacacggat ggggacctgc tcgttggaca tgccgggtgc tgccacgact gaaccacctc
    14641 acggatagat gggcttacat ccgtgaccat agatgtttgt cacggatgaa ggcaagctgt
    14701 accgtgagat acgtgagtga gatcgacacc gccgcccctg ccgaaaccgc tgcccccggg
    14761 ctgccgcagg caccgggcgc tgggcaatac cccgcgctcg acctcgccaa cagcgctatc
    14821 gccctgccgg gtggccagtt cctcgatgcg ttgggcacgc ccgccggcgc caaccagtgg
    14881 ctggccgacc acaacctggc ccccgcggac gccggcctgc aggagatctg tgcggcacgc
    14941 gtgcgttccc tgcgcgaaca ggtacgagcc ttgctcgccg cccaagtagg cgggcatccg
    15001 gctccggcaa ccgcgctggc tgccgtcaac gacgccctca cacgggtccc caccgcctcc
    15061 ccgctcggct gggatgcggt ccgcggcatg caccgcaccg ccccgcaccc catcgaccag
    15121 atcgtcgacc aggccctcgg catcctcgcc gccgacgccg ccgacctgct caccgggccc
    15181 gacgccgaac ggctgacggc ctgcccctcc cacccctgca accgctacct gctccgtgcc
    15241 ggacgccgtc actggtgctc ggtccgctgc ggcgaccggg cccgcgccgc ccgcgcctac
    15301 gcccgccgca cgcagaccac cgccgactga gttcggccac ccccaccccg ccgccggttc
    15361 accggacccg cgccggggcg cggcttcgca catcctcaag cccccggcac ggacggcaca
    15421 cctcgccctc cctgccggcc attcccacga gcaacaaagg accatcacct ctcatgaccg
    15481 ccacattcac cctgctgacc accggctacg gcaggctcga cgcacccaat gacggccgcg
    15541 acggccgcga gaagccgtgg tacgtcgcct cgaccgtcgg cctcctgtgc gacggcgaca
    15601 cgatcgtcgt cgtcgacccc ggcatggtcg aggaccgcaa actcatcctc gatccgctcg
    15661 ccgccgccgg aatcaccccc gagcaggtga ccgacgtggt cttcagccac caccaccccg
    15721 accacaccct caacgcagcc ctcttcccca acgcgcgcgc ccacgacgtc tgggccgcct
    15781 accacaacca cctctggtac gaccggcccg ccgacggatg ccagctctcc ccctcgatcc
    15841 gcctgatcga aacgcccggc cacacccccc aggacatctc caccgtcgcg gaaaccgccg
    15901 aaggcaccgt cgtcctcacc cacgcctggt ggagcgtcca cggccccgcc gaagacccca
    15961 cctccgacga ccccgccggc ctgagcgcct cccgccgcaa gatcctcgcc ctcaagcccg
    16021 ctctgatcgt ccccggccac ggagacccct tcgtccccga cgagaacacc cccatctgac
    16081 ctacagatat caacccgaag accggcttcg ccgtcgtcat ccggtgctcg atcgcggtac
    16141 gcctgctgga tctggccccg gcatcggacg atcaccccag gccaggcggc cccggatgtg
    16201 cgctgtcgtg gggtcctcgt cggcagtacc cggacgacac cggcacggtc ggccagcggc
    16261 gtgcgcgggc tgagcatcgg tgaggcggtc agcgcggtgc cgtccttcac catgaccgac
    16321 tattccgtgc acggggaggc ggttctcgcc cccgcccacg cggtcgtcgc ccacccgagc
    16381 ggctctcctt cgccgggcga gggcgacagc ggtgtttgcc gggccgccac cggcaagcgc
    16441 acgcaggcca gaccgccggc atcgggccgg tcgcggtcct cgaagatgtc agcggcgcac
    16501 tcgccgagga cgtcgacgcg gccgggggtc cccccgtcgg gccaaggtga ctgtctgcct
    16561 ctcaggtagg gcgcggggcc gtagccccgc gccacggaac gcagcccgca ggcccgtagg
    16621 ggcatggggt gctgcaatgg ccacgtctgc cgcacacggt gcgagatcac ggggcctgta
    16681 ggtgtgcggg tgggtcagcc ttcgggccgc gtgcggccca gggcatcagg cacgctgtgc
    16741 tgtccggggc gccggctcgg cttcctgccg gatgtcctgc ggggtcaggt tccaggccgc
    16801 gacggcgaac tcgctgcgcc ctgtccctgt cgtacgggcc tgcagccgcc acggtccgtc
    16861 ggtggacggg tagaacctca gggtgaggac ctgctcgtcg gccaggtaga cctcggctat
    16921 ggaacggtcc acgatcacgc gaagttcgac gggggtgtgg agacgcgccg ctgccgggca
    16981 cggtgcggtg taggcccctc cccgtgcccg cgtgtccggt gaggcgtggg tgcggtcgac
    17041 ggcgaggcgg cctgccgtgg ggtcgagact gatgtcgagg tactcggagc cgtcggtcga
    17101 cgtgatcagg cgcagcccgc tggtaccggt ggtgtccggg acgagggtgg cggtgaggtc
    17161 gaaggtgtgg ctgacctggc cgagttcgac gggttcggtc cggctgacgt gtccggttcc
    17221 gtacaggacg cgcgggccgc gcagggccag cagttcgcgg gcggggcgct gggcgaccgt
    17281 gccgtcggcg gtgaggctga cctcacgggg gagggtgagg acgcctgccc agcccgcttc
    17341 gtgcgcccag gcgtcgtccc gggcttccca tgcccagccc cacatcagcc agcggtcttc
    17401 tcctggggcc ttcagcaggg cgggggcgta gaagtcgggg ccgtggtcca gcggcacgag
    17461 ggccgtggcc gcggtgaagc gtccgtcctt ctcctggccg atgatgacgc tggtgtggct
    17521 gggtccgccc tgaggagtcc agtcgctgag gatcagaaca ccttggccgc cgaaggtggc
    17581 gtactggggg cactcccagc cgatggggcc cgtgccggtc gcggcgtcgc tggtgtggaa
    17641 ggggcctcgg taggtccagt gctccaggtc gtcggactcg tacagttgcg ccgcggcgcg
    17701 gccgtcgtcg agggcggatc cgacgagcat ccgccagcgt tcgtcctgcc gccagacgta
    17761 ggggtcccgg tacatggtgg tgccggcggg cggttcgggg atgagcaggt gggggcgttt
    17821 ggcccaggtg cggccgttgt ccgacgactc cgcggtcgtg atcggctgcc accagcggtc
    17881 attgcggtgg gcggagtaga aggccaccat gcggttgcct tcggagacgg cgttgcccga
    17941 gtaacagccg tcggcgtcgt ggccgccggg ggtgggggtg agggcgacgg ggaggggttc
    18001 ccaggtgatc aggtcggggc tgcggtagtg gccccagtgc atgttcgcgt gctgggggcc
    18061 gtgcgggttg tactggaaga agacgtggta gtggccgtcg tggaagacca gaccgttggg
    18121 gtcgttgatc cagttgcggg gcggtcgcag atggacggtg ggcaggtggg ggtcggcggg
    18181 cgacgtggac acacggggcc tttctcaggg cgtgcgaagc gcacggaagt cgtgaggacg
    18241 aggcggggtc gccagtgcgg tgcaggggtc agcccttgac gccggtggag gcgatgctgt
    18301 tgacgaacga ccgctggaac gccacgaaca gggcgaggat cggcacggtg atcatggagg
    18361 agtaggccat gatctggccc cagcccacgt tcagctggaa gaagtaggag atgccgacca
    18421 tcaccggccg cagctcttcg ctctgtacga ccatcagcgg ccacaggtag gagttccagg
    18481 cgggcaggaa ggtgaggatg gcgacggtgg cgacggccgg gccggacagg ggcatcacga
    18541 tgcggcggta gatggtgaac cagccggcgc cgtccatgat cgccgcttcg tcgagttcct
    18601 tggggatgct ctggaagaac tggtggaaga agaagatcga gaaggcgttg gcgacgaacg
    18661 ggaggacctg cacctggtag gtgtccatcc agccggtggt ccacgccagg tggaagccgt
    18721 tcacgcggag cagcggcagt tggttgaccc accacaccag cggcagcgcg aaggtctcga
    18781 acggcacgat gagggtggcg atgatcacgg tcagcacgag cttgcggccg ggccaccgca
    18841 tccgtgagag cgcgaaggcg gccaggctgt tgacgatgat gccgagcacc acggtgaccg
    18901 acgagatcag cacggagttc agcaggaaac gtgccgccgg gacacgctcg aacatgtcgg
    18961 tgtagttgtc cagcgacagg gttccgaccg gcaggaaggc gcgcagtgag ccgaggtcgc
    19021 cgaagatctg gtcgtccggt ttgaacgacg agacgagcat gaagaggagc ggcagcgcga
    19081 agaccagggc caggacgacg cggccggcgt gaccgctgag ccgcttgagg agggaggtca
    19141 tgtcagtcct tctcccgggt caggaagcgc tggacgagcg acacggtcag gacgagtacg
    19201 aagaagatca gcgagatggc cgccgcgtag gcggtctgct gctggtcgta gccggtgcgt
    19261 acggcctggt agacgacggt ggtggtggag tcgagcggac cgccctgcgt catgaccctg
    19321 atctgggtga agaggctgaa ggcggcgatc gtgatggtga cgagcacgaa ggtgcgggtg
    19381 gcgcgcagtc ccggccaggt gacgtggcag aagcggtgcc agggtgtggc gccgtcgatc
    19441 tcggcggctt cgtacagctc ggcggggatg gtctgcaggc cggccagcca gatgatcatg
    19501 tggaagccga cgccctgcca caccgacatc aggatgatgg ccggcatcgc cgtggccggg
    19561 tcgcccagcc agtccgggcc ctggaagtgg ccgagggtga gtgtcgagat gacgtggttg
    19621 accaggccgt ccttccggta gaggaaggtc cacaggaggg acaccacgac catcgaggtg
    19681 acgacgggca ggaagtagac ggtgcggaag aagttgacgc cccgtacttt cgcgttgacc
    19741 agcagcgcca ggaccagggc gaggccggcc tggagcggaa cgacgacggc ggcgaagtac
    19801 gcggtgttgc gcagggactt gtagaagacc gggtcttcga agagccgggt gaagttgcgc
    19861 aggcccacga aacgggccgg ggtgggcgag atgagcctcg cgtcggtgaa ggccagggcg
    19921 aaggcgaggg cgaccggtac gacgaggaag acgatcagca gcacgacggc cggggtggcc
    19981 atgccgaggg cggtgaggct ctcacggcga cgggtgttgg tcgttcgggt tcgccggacc
    20041 gggcgcagtc gctcgggagc tgcggttctg gtggtcacgg tatctccagg gagccggtcc
    20101 ggttcggacg gcgtggtcgg ggaccgaacc ggaccggggc gggtcagcct gcgtagttgt
    20161 tgttcgtctt caggttgttg tcgatgttct tcgcggcctg gccgagagcg tcctcggggt
    20221 cggcgccggc caggatgtcc tgggcggcct tctggaactc ggtggagatg tacgggtagg
    20281 ccggggtgac cggacgcacc gtggcgtact cgcgtgcgaa ctggacgagt tcctcgaaca
    20341 tgccgcccgg ctggtagtcc gcgatctgct gagcggcggc gtccgtggcg gggatggtgc
    20401 tggtggcctg ggcgtagtcg acgaagtact tggtctggcg ggagaacttc agccactcct
    20461 gggcgccgtc cttgttggag caggtggagc tcatggccca ctgccaggag gagccgccga
    20521 tcttgggtcc gttgccgaag tcgaccggcg ggatgatcgc caggtcgtcg ccgagcttgc
    20581 cggcgttctt ggccgcgttc cagctgccgt cccactggat ggcgctcttg ccggccagga
    20641 agtccttgtt ggggtcggct ccggacttct tcgccatgta gcccttggtc accagcgagc
    20701 ggaaccagtt gccccactcg acggactcct tgccgtcgag gatgccggag gcggccttgt
    20761 agccgttgcg gtcgatgagg tcgccgccga agctctgcag ctgcggggag taggcgtacg
    20821 gccaccactc gccgctgccg ccggtgccca tctccagcgg gtagtcgaac tcgccgctct
    20881 tcttcagctc ggccagcgcg gtgtcgaact cactcctggt ccagggcttg tccatggtgg
    20941 ggatgcggat gccgtgctcg tccaggacgg acttgcgggc gaagaaggcc agggagacgt
    21001 cgtagaagcc gaaggagtag agctggtcct tgtacgtgcc gacggtgctg gcgagctggt
    21061 cctccaccgg cacctcgctg ccggtgacgt cgatgggggc gaggtagccg ccccaggccc
    21121 agttcgccac gttggggccg tccacgtcga gcaggcaggg cagcttg
//
