GCF_001267805.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP010449.1 (Streptococcus pyogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	azole-containing-RiPP	568,135	591,901	High	streptolysin S	ribosomal:RiPP:LAP
Region 2	T3PKS	688,374	729,549
Region 3	lanthipeptide-class-i	817,357	841,586	High	streptin	ribosomal:RiPP:Lanthipeptide
Region 4	terpene-precursor	1,295,206	1,316,078
Region 5	RiPP-like	1,554,918	1,566,763

NZ_CP010449.1 - Region 1 - azole-containing-RiPP

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Location: 568,135 - 591,901 nt. (total: 23,767 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000566.5	0.98	ribosomal	streptolysin S	Streptococcus pyogenes M1 GAS
BGC0000564.5	0.69	ribosomal	clostridiolysin S	Clostridium botulinum A str. ATCC 3502
BGC0001170.5	0.64	ribosomal	clostridiolysin S	Clostridium sporogenes ATCC 15579
BGC0001171.5	0.50	ribosomal	listeriolysin S	Listeria monocytogenes serotype 4b str. F2365
BGC0000569.5	0.49	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001173.5	0.48	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0002700.2	0.41	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000605.5	0.40	ribosomal	GE37468	Streptomyces sp. ATCC 55365
BGC0001887.3	0.39	ribosomal	huazacin	Bacillus thuringiensis serovar huazhongensis BGSC 4BD1
BGC0002111.3	0.39	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000566.5	0.98	ribosomal	streptolysin S	Streptococcus pyogenes M1 GAS
BGC0000564.5	0.69	ribosomal	clostridiolysin S	Clostridium botulinum A str. ATCC 3502
BGC0001170.5	0.64	ribosomal	clostridiolysin S	Clostridium sporogenes ATCC 15579
BGC0001171.5	0.50	ribosomal	listeriolysin S	Listeria monocytogenes serotype 4b str. F2365
BGC0000569.5	0.49	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001173.5	0.48	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0002700.2	0.41	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000605.5	0.40	ribosomal	GE37468	Streptomyces sp. ATCC 55365
BGC0001887.3	0.39	ribosomal	huazacin	Bacillus thuringiensis serovar huazhongensis BGSC 4BD1
BGC0002111.3	0.39	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.45 out of 26.06

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.98 out of 20.97

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.03 out of 20.97

Confidence: medium

Additional weak hits (3):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 22.53 out of 33.73

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.72 out of 30.22

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.38 out of 27.18

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

RREFinder

TFBS Finder

Azole RiPPs

RRE predictions

: RRE type: Cytolysin_C_RRE; Location: 0 - 90; Score: 134.5

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CcpA	Catabolite control protein A
MogR	Repressor of flagellar motility genes

Core peptide predictions

No core peptides found.

NZ_CP010449.1 - Region 2 - T3PKS

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Location: 688,374 - 729,549 nt. (total: 41,176 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: reductase

SD89_RS03475: hydroxymethylglutaryl-CoA reductase

smcogs: SMCOG1187: hydroxymethylglutaryl-CoA reductase

Hydrolases: amidase deformylase esterase

SD89_RS03430: peptide deformylase

smcogs: SMCOG1275: peptide deformylase

SD89_RS03435: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

smcogs: SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

SD89_RS03575: amidase

smcogs: SMCOG1105: amidase

Ligases: synthase

SD89_RS03480: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002561.3	0.44	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000286.3	0.40	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001080.5	0.40	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000934.4	0.39	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0002284.3	0.39	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002362.3	0.38	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0001863.4	0.38	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0002701.2	0.38	ribosomal	paenithipeptin	Paenibacillus thiaminolyticus
BGC0001521.3	0.38	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000679.5	0.38	terpene	diazepinomicin	Micromonospora sp. M42

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002561.3	0.44	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000286.3	0.40	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001080.5	0.40	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000934.4	0.39	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0002284.3	0.39	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002362.3	0.38	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0001863.4	0.38	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0002701.2	0.38	ribosomal	paenithipeptin	Paenibacillus thiaminolyticus
BGC0001521.3	0.38	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000679.5	0.38	terpene	diazepinomicin	Micromonospora sp. M42

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.33 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.93 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.30 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.60 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.77 out of 27.78

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.20 out of 24.78

Confidence: medium

Additional weak hits (1):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 22.77 out of 28.14

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
LexA variant 2	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes

NZ_CP010449.1 - Region 3 - lanthipeptide-class-i

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Location: 817,357 - 841,586 nt. (total: 24,230 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

SD89_RS04050: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

SD89_RS04055: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000556.5	0.95	ribosomal	streptin	Streptococcus pyogenes
BGC0000538.3	0.56	ribosomal	nisin Z	Lactococcus lactis
BGC0000541.5	0.53	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000542.3	0.53	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000559.4	0.52	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0002323.2	0.51	ribosomal	nisin J	Staphylococcus capitis
BGC0000537.3	0.50	ribosomal	nisin U	Streptococcus uberis
BGC0001789.3	0.50	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000626.3	0.50	ribosomal	thuricin	Bacillus thuringiensis
BGC0001289.5	0.50	ribosomal	penisin	Paenibacillus ehimensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000556.5	0.95	ribosomal	streptin	Streptococcus pyogenes
BGC0000538.3	0.56	ribosomal	nisin Z	Lactococcus lactis
BGC0000541.5	0.53	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000542.3	0.53	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000559.4	0.52	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0002323.2	0.51	ribosomal	nisin J	Staphylococcus capitis
BGC0000537.3	0.50	ribosomal	nisin U	Streptococcus uberis
BGC0001789.3	0.50	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000626.3	0.50	ribosomal	thuricin	Bacillus thuringiensis
BGC0001289.5	0.50	ribosomal	penisin	Paenibacillus ehimensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.71 out of 20.97

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.55 out of 19.26

Confidence: medium

Additional weak hits (1):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.51 out of 30.25

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 134 - 219; Score: 41.5

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A

NZ_CP010449.1 - Region 4 - terpene-precursor

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Location: 1,295,206 - 1,316,078 nt. (total: 20,873 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

SD89_RS06495: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002698.2	0.40	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000487.5	0.36	ribosomal	carnocyclin	Carnobacterium maltaromaticum
BGC0000542.3	0.33	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.33	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000502.5	0.33	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001859.4	0.32	other (phosphonate)	fosfomycin	Pseudomonas syringae
BGC0000617.5	0.32	ribosomal	coagulin	Bacillus coagulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002698.2	0.40	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000487.5	0.36	ribosomal	carnocyclin	Carnobacterium maltaromaticum
BGC0000542.3	0.33	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.33	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000502.5	0.33	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001859.4	0.32	other (phosphonate)	fosfomycin	Pseudomonas syringae
BGC0000617.5	0.32	ribosomal	coagulin	Bacillus coagulans

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

SD89_RS06495: PT_FPPS_like

Domain 1 (25 - 272): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.70 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.52 out of 26.06

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.59 out of 30.25

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.00 out of 26.85

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.16 out of 20.97

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.93 out of 26.33

Confidence: medium

Additional weak hits (1):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.95 out of 39.62

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CcpA	Catabolite control protein A
ColR	Phenol-responsive regulator
MogR	Repressor of flagellar motility genes
RutR	Regulator of the pyrimidine and purine metabolism

NZ_CP010449.1 - Region 5 - RiPP-like

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Location: 1,554,918 - 1,566,763 nt. (total: 11,846 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	RiPP-like	Similarity score	Type	Compound(s)	Organism
BGC0000526.5		0.22	ribosomal	macedocin	Streptococcus macedonicus

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0000526.5		0.22	ribosomal	macedocin	Streptococcus macedonicus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.12 out of 23.79

Confidence: medium

Additional weak hits (3):

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.42 out of 29.72

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.57 out of 26.85

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 18.45 out of 30.12

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism