LOCUS       NZ_CP010449            20873 bp    DNA     circular CON 21-JAN-2024
DEFINITION  Streptococcus pyogenes strain NGAS322 chromosome, complete genome.
ACCESSION   NZ_CP010449
VERSION     NZ_CP010449.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1950469)
  AUTHORS   Fittipaldi,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2015) Public Health Ontario, 661 University Ave,
            Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera v. 7.0; Quiver
            Genome Coverage                   :: 114X
            Sequencing Technology             :: SMRT PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/21/2024 01:43:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,960
            CDSs (total)                      :: 1,871
            Genes (coding)                    :: 1,812
            CDSs (with protein)               :: 1,812
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 29 of 59
            Pseudo Genes (incomplete)         :: 33 of 59
            Pseudo Genes (internal stop)      :: 21 of 59
            Pseudo Genes (multiple problems)  :: 20 of 59
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:43:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1295205
            Orig. end                         :: 1316078
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP010449.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA available from Nahuel Fittipaldi,
            Nahuel.fittipaldi@oahpp.ca.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20873
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="4"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20873
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20873
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10873](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10873)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            complement(26..238)
                     /locus_tag="SD89_RS06435"
                     /old_locus_tag="SD89_06455"
     CDS             complement(26..238)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922479.1"
                     /locus_tag="SD89_RS06435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06455"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_010922479.1"
                     /transl_table=11
                     /translation="MVTIAELRAKHNKMSQRELAKELGVTQTSISNWEKDQTKISGEYL
                     ISLALFFDVSTDDILGINKQATQKF"
     misc_feature    complement(324..337)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.7"
                     /tool="antismash"
     misc_feature    complement(325..338)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.52"
                     /tool="antismash"
     misc_feature    complement(329..344)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.59"
                     /tool="antismash"
     gene            440..1195
                     /locus_tag="SD89_RS06440"
                     /old_locus_tag="SD89_06460"
     CDS             440..1195
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922480.1"
                     /locus_tag="SD89_RS06440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06460"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_010922480.1"
                     /transl_table=11
                     /translation="MEQLGDRIRKLREGRNMTQTELSEILGMKTYTTVSKWEKNENFPK
                     GKDLKKLAEIFNVTSDYLLGLTDSKLGKITIQNEQPEIVSIYNQLEQPRQEKVLNFANE
                     QLEEQNKTVSIFDKKSEETEDYITDYVEGLVAAGLGAYQEDNLHMKVKLRSDDVPDEYD
                     TIAKVAGDSMEPLIQDNDLLFIKVSSQVDMNDIGIFQVNGKNFVKKLKRDYDGAWYLQS
                     LNKSYEEIYLSKDDDIRTIGEVVDIYREY"
     gene            1207..1725
                     /locus_tag="SD89_RS06445"
                     /old_locus_tag="SD89_06465"
     CDS             1207..1725
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922481.1"
                     /locus_tag="SD89_RS06445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06465"
                     /product="HIRAN domain-containing protein"
                     /protein_id="WP_010922481.1"
                     /transl_table=11
                     /translation="MAKVIFQDNFLLMGTNYHEKEANKVMAEIGKKSPYWDKDKDFISD
                     YIKSNFKDIYKYYRVSTKDVEIVREPLNRHDPNAIKVMVNKTFVGYFPADLAKRLTPYV
                     KKSSHYQMEATLTGRGGQYKTLKNDLKTVVTKKKDITYKLRLTILKVDRVSKSKNAGLL
                     ESIASWFLN"
     gene            1849..2991
                     /locus_tag="SD89_RS06450"
                     /old_locus_tag="SD89_06470"
     CDS             1849..2991
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /locus_tag="SD89_RS06450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06470"
                     /product="site-specific integrase"
                     /protein_id="WP_053308491.1"
                     /transl_table=11
                     /translation="MWIEETDNGKFKFRENYKDPYTGTWKPVSVTMEKDNSRAYKAARK
                     ILEQKITEKIAQLKASELLFTELLDEWWAFYKKELKRSSVASLRGNIEEIREAFGIGVK
                     VVNIDPKYVQNYLDNLDCSRNKKERNKSMLNLAFDYAVGLDIIQDNPARRAKLPRVKKT
                     LEDWKKAGEKYLEEDEIKPLLKELYRRPSTYRLGLLAEFISLNGCRIGEAVSIEPCNYE
                     SKSRILQLHGTFDHTEGYRNGEKTAPKTLASYRETIMTSRELEILQELEFMNELEKNTN
                     HRYRDMGYLFTTKNGVPIQTNSFNLALKKANERLEDPITKKLTSHIFRHTLISRLAENN
                     VPLKSIMERVGHADAKTTAQIYTHVTKKMKSSVADIMENY"
     gene            complement(3081..3356)
                     /locus_tag="SD89_RS06455"
                     /old_locus_tag="SD89_06475"
     CDS             complement(3081..3356)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1132: DNA-binding
                     protein HU 1"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /locus_tag="SD89_RS06455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06475"
                     /product="HU family DNA-binding protein"
                     /protein_id="WP_002983920.1"
                     /transl_table=11
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQL
                     IGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(3455..4042)
                     /locus_tag="SD89_RS06460"
                     /old_locus_tag="SD89_06480"
     CDS             complement(3455..4042)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /locus_tag="SD89_RS06460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06480"
                     /product="YpmS family protein"
                     /protein_id="WP_002989129.1"
                     /transl_table=11
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIAK
                     KPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYEVP
                     LYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSAIVV
                     NLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            complement(4020..4862)
                     /locus_tag="SD89_RS06465"
                     /old_locus_tag="SD89_06485"
     CDS             complement(4020..4862)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /locus_tag="SD89_RS06465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06485"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_011888746.1"
                     /transl_table=11
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKEQ
                     VAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQILDR
                     MIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQIIEL
                     ARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNIKTKEVVGEYDRVYFVPINDLL
                     YKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEKKIKP"
     gene            complement(4855..5694)
                     /locus_tag="SD89_RS06470"
                     /old_locus_tag="SD89_06490"
     CDS             complement(4855..5694)
                     /GO_function="GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264715.1"
                     /locus_tag="SD89_RS06470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06490"
                     /product="DegV family protein"
                     /protein_id="WP_002989125.1"
                     /transl_table=11
                     /translation="MGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSDNDLKE
                     EGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTIEASRQG
                     AEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTELYIGVST
                     LENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLDSYLAKNSH
                     RPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAFAVMVRYE"
     gene            complement(5922..6869)
                     /locus_tag="SD89_RS06475"
                     /old_locus_tag="SD89_06495"
     CDS             complement(5922..6869)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /locus_tag="SD89_RS06475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06495"
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_053308492.1"
                     /transl_table=11
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVEK
                     DVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDRLL
                     NELDESDEEDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLRS
                     LSDTALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAHYE
                     DRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYSRKLGMAVAKRKI
                     ALPQTGEGFSYYPALLGLMILGLTPIMIPKKINN"
     gene            complement(7041..8702)
                     /gene="recN"
                     /locus_tag="SD89_RS06480"
                     /old_locus_tag="SD89_06500"
     CDS             complement(7041..8702)
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /locus_tag="SD89_RS06480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06500"
                     /product="DNA repair protein RecN"
                     /protein_id="WP_002983909.1"
                     /transl_table=11
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNMM
                     LGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGRSV
                     SRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKENYQL
                     IFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDRLMNH
                     KQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYYILEEV
                     SKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEYQLLTGD
                     DLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKADFKVHFT
                     TSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKEDKTSIVFD
                     EVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKESKEESTVSKV
                     RLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            complement(8724..9194)
                     /locus_tag="SD89_RS06485"
                     /old_locus_tag="SD89_06505"
     CDS             complement(8724..9194)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /locus_tag="SD89_RS06485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06505"
                     /product="arginine repressor"
                     /protein_id="WP_002983907.1"
                     /transl_table=11
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRDM
                     NEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVVPG
                     NSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            complement(9181..10008)
                     /locus_tag="SD89_RS06490"
                     /old_locus_tag="SD89_06510"
     CDS             complement(9181..10008)
                     /EC_number="2.1.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /locus_tag="SD89_RS06490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06510"
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="WP_003056952.1"
                     /transl_table=11
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPGD
                     KIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQDG
                     AKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVFASIDVSFISLSL
                     ILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASGYGFT
                     VKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQKVVAKAHKEFEKHEKE"
     gene            complement(10001..10873)
                     /locus_tag="SD89_RS06495"
                     /old_locus_tag="SD89_06515"
     CDS             complement(10001..10873)
                     /EC_number="2.5.1.10"
                     /GO_function="GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /locus_tag="SD89_RS06495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06515"
                     /product="farnesyl diphosphate synthase"
                     /protein_id="WP_011284950.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 4e-51, bitscore:
                     170.6, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPLI
                     LLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGEAT
                     AILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQALSL
                     PQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILDVTAS
                     FEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKPQIITK
                     LIEGLRLNA"
     gene            complement(10873..11088)
                     /locus_tag="SD89_RS06500"
                     /old_locus_tag="SD89_06520"
     CDS             complement(10873..11088)
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /locus_tag="SD89_RS06500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06520"
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_002983901.1"
                     /transl_table=11
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKEL
                     QKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            complement(11066..12406)
                     /gene="xseA"
                     /locus_tag="SD89_RS06505"
                     /old_locus_tag="SD89_06525"
     CDS             complement(11066..12406)
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /locus_tag="SD89_RS06505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06525"
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_053308493.1"
                     /transl_table=11
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQYF
                     SLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDGIG
                     ALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRFPGV
                     EILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVVQAIF
                     ESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQNRSYQA
                     CLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSVKENKVQ
                     LDHSLANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLSLDASRII
                     ARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            complement(12559..13413)
                     /locus_tag="SD89_RS06510"
                     /old_locus_tag="SD89_06530"
     CDS             complement(12559..13413)
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /locus_tag="SD89_RS06510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06530"
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="WP_010922486.1"
                     /transl_table=11
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDPA
                     SQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPNHI
                     NDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKHAVI
                     IGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQYIKD
                     GAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQSALRS
                     THK"
     gene            complement(13621..15315)
                     /locus_tag="SD89_RS06515"
                     /old_locus_tag="SD89_06535"
     CDS             complement(13621..15315)
                     /EC_number="5.4.2.-"
                     /GO_function="GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /locus_tag="SD89_RS06515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06535"
                     /product="phospho-sugar mutase"
                     /protein_id="WP_002989112.1"
                     /transl_table=11
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEFG
                     TAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFAEL
                     TCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQEGS
                     QILDDIADQIAQHMAALTQYQEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGLTIND
                     TDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPNPEVPK
                     TFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYYIFSQRF
                     DLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVTKQKNYLF
                     GYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATFGYYNERQI
                     ALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQNCLKFYLDDG
                     SWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     misc_feature    complement(15333..15348)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.0"
                     /tool="antismash"
     misc_feature    15366..15377
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.16"
                     /tool="antismash"
     gene            complement(15493..16902)
                     /locus_tag="SD89_RS06520"
                     /old_locus_tag="SD89_06540"
     CDS             complement(15493..16902)
                     /EC_number="4.1.99.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /locus_tag="SD89_RS06520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06540"
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="WP_030126394.1"
                     /transl_table=11
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLHS
                     RNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGYGR
                     LRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKETPIK
                     VELSQGRWLNLETPDDVLMTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHANRDF
                     PAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYVAYPDQ
                     KTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRMIVASFL
                     TKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVTQGKRFDP
                     KGEFIKAYLPQLEHVPEKYLHEPWKMPKNLQESVSCIIGTDYAQPIVDHAKQREQAIAK
                     YEWAKEKAKIE"
     gene            complement(17051..17785)
                     /locus_tag="SD89_RS06525"
                     /old_locus_tag="SD89_06545"
     CDS             complement(17051..17785)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /locus_tag="SD89_RS06525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06545"
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="WP_002989107.1"
                     /transl_table=11
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGKS
                     TLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENITL
                     SPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPDVLL
                     FDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVIVEEG
                     SPNQLFDLTKEERTKDFLSRVL"
     gene            complement(17785..18471)
                     /locus_tag="SD89_RS06530"
                     /old_locus_tag="SD89_06550"
     CDS             complement(17785..18471)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /locus_tag="SD89_RS06530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06550"
                     /product="amino acid ABC transporter permease"
                     /protein_id="WP_053308494.1"
                     /transl_table=11
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRSH
                     VKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIIIIS
                     LNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNEFIT
                     IIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLIVTTVMAQLLAVLERHM
                     AQGGNH"
     gene            complement(18598..18828)
                     /locus_tag="SD89_RS06535"
                     /old_locus_tag="SD89_06555"
     CDS             complement(18598..18828)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /locus_tag="SD89_RS06535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SD89_06555"
                     /product="YkuJ family protein"
                     /protein_id="WP_002983885.1"
                     /transl_table=11
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPENG
                     PVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     misc_feature    complement(19202..19215)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    20089..20109
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.95"
                     /tool="antismash"
ORIGIN
        1 tatatgattt aaaatcatat agtgtttaaa atttttgtgt tgcttgcttg tttatcccaa
       61 gaatgtcatc ggtagataca tcgaaaaaca aagctagact aataagatac tcacctgaaa
      121 tctttgtctg gtctttttcc cagttgctga tagatgtttg agtaacacct aattcttttg
      181 ccaattcacg ctgtgacatc ttattatgtt tagctcttaa ttctgcaata gttaccatag
      241 ctgagtcgct cctttcttga tgatgattac attctatatg attttaaatc ttgcgtcaag
      301 tgttttatat gatttatttt ctgttttttt gaaaaaatcg taaatcatat agtttattta
      361 aaactttttt cttatatagt tgatttataa tcatggtttt gatataattt acttatcaaa
      421 attaaaaagg aggtatccta tggagcaatt aggggaccga ataagaaagt tgagagaagg
      481 gcgcaacatg actcagactg aactttcaga aatattgggc atgaaaacct atactactgt
      541 ttcaaagtgg gagaagaatg aaaattttcc aaaaggtaaa gacctaaaaa aactagcaga
      601 gattttcaac gttacctctg actatctttt aggactcaca gatagtaaac ttgggaaaat
      661 cacaatacag aacgaacaac ctgaaatcgt ttctatatac aaccaactag aacaacctag
      721 acaagaaaaa gtcctcaact tcgctaacga acaattagaa gaacaaaata aaaccgtttc
      781 tatattcgat aaaaaatctg aggagacaga agattatatc actgactacg ttgagggatt
      841 ggttgccgct ggtcttgggg cataccaaga agacaattta cacatgaaag ttaaactacg
      901 ttctgatgat gtccccgatg agtatgatac tattgcaaaa gtagctggcg attcaatgga
      961 accacttatt caagacaacg atttgctatt catcaaggta tctagccaag tcgatatgaa
     1021 tgatataggg atattccaag tcaatggtaa aaactttgta aaaaaactca agcgtgatta
     1081 tgatggcgct tggtacttgc aaagtttaaa taaaagctat gaggaaatct atctatcaaa
     1141 ggatgacgac atccgcacaa tcggtgaagt tgtggatatt tatagggaat attaaaggag
     1201 actgttatgg caaaagtcat attccaagat aactttctct taatgggaac aaattatcat
     1261 gaaaaagaag ctaataaagt tatggctgaa attggtaaga aatcaccata ctgggataag
     1321 gataaagatt ttatttctga ctacataaaa tcaaacttca aggatatcta caagtattac
     1381 agggtctcaa caaaagatgt agagattgtc agagaacctc ttaatcgtca cgatccaaat
     1441 gccataaaag tcatggtaaa caaaactttt gttggctact ttccagcaga cttagctaaa
     1501 cgattaaccc cttatgtaaa aaaatctagt cattaccaaa tggaagcaac gcttactggt
     1561 cgtggtggac aatacaaaac actcaaaaac gatttaaaga cagtggttac taaaaagaaa
     1621 gatataactt ataagttacg attgactatt ttaaaagtag atagagtatc aaaaagtaaa
     1681 aatgctggat tattagagtc gatagcatct tggtttctca actaacaaaa aagccccacg
     1741 ctcaaatttg gcgaggagag cgtgaggcga atctagtata gtaaaaacct gcttcgcagt
     1801 aggtctcttt actataccta ttttaacaga aaatgaggta aaaaacaaat gtggatagaa
     1861 gaaactgata acggtaaatt caaatttcgt gaaaattata aagatcctta cactggaaca
     1921 tggaaacctg tatccgttac tatggagaag gataattcaa gagcttataa agcagctcga
     1981 aaaatccttg aacaaaaaat aacagaaaaa atagcgcaat taaaggcctc tgagttactt
     2041 ttcacggaac ttttagatga atggtgggcg ttttataaga aagaacttaa aaggtcgtct
     2101 gtagcttctc tgaggggtaa catcgaagag ataagggaag cttttggaat aggtgttaaa
     2161 gtagtgaata ttgatcctaa atacgttcag aattatctcg ataacctaga ttgctctagg
     2221 aataaaaaag agcgtaataa gtctatgcta aacttagcat ttgattatgc tgttggtttg
     2281 gatattatcc aagataatcc tgcaagacgt gctaaactcc caagggtaaa gaaaactctt
     2341 gaagactgga aaaaggctgg agaaaaatat cttgaagaag atgaaattaa accattattg
     2401 aaagaattgt acagaagacc tagcacttac cgtcttggtt tgttggctga gtttatcagt
     2461 ttgaacggtt gtcgtatcgg tgaagctgtc agcattgagc cgtgcaacta cgaatctaag
     2521 tcaagaatat tgcagttgca cggaacattt gatcatacag aaggatatcg taacggggaa
     2581 aagacagcac caaagacatt agcttcttat cgtgaaacca tcatgacaag tagagaactt
     2641 gaaattctac aagagttaga atttatgaac gaactagaaa aaaatacaaa tcatagatat
     2701 agagatatgg gatacctttt tacaacaaaa aacggtgttc caattcagac taactcattc
     2761 aacttagctt taaaaaaagc taatgaaaga ttggaagacc caattacaaa aaaacttact
     2821 agccacatct tccgccacac tcttattagc cgtttagcag agaataacgt gccgctaaaa
     2881 tcaatcatgg aacgtgtagg acatgcagac gctaaaacta ctgcccaaat ttatacgcat
     2941 gtcacaaaga aaatgaaatc aagtgtagct gatattatgg aaaactatta aattttttac
     3001 ccctttttta cccccctgaa cacaaaaaag cccactgcac aggctgtaac gcttgatgca
     3061 atgggctttt atctatgcaa ttatttaaca gcgtctttaa gagctttacc agctttgaag
     3121 gctggaactt ttgaagctgc aatttcaatt tctgcaccag tttgtgggtt acgaccttta
     3181 cgagctgcac gttcgcgtac ttcgaagtta ccaaaaccga tcaattgtac tttttcacct
     3241 tcagcaagga aagcttcgat tgtagaaaac acggcatcaa ctgctgctgc tgaatctttt
     3301 ttagtcaatt cagttgcttc tgcaaccttt gcgattaaat cttgtttgtt agccatttaa
     3361 caaatcctcc aaataattag taagcttaat gctttaacag atattatcat atctaaaaaa
     3421 agcgattagg tcaagtattt aacgttattt gagcttattt cttatagatg ttaaaactga
     3481 tttcatcatt aaataaatca attttcttgg cctttaaata aaccttagca tcattttgga
     3541 tatcttgtaa attaacaacg atagctgatt gatttggcat cactttaaca aagcttggca
     3601 atttgtagct tgatttcaaa tactgcaaaa catctttttc tgggagtggc agagttccca
     3661 ctgaaaaaga gatcacctgt agttggacag ctccattttc tagacgatga ggttgaaaat
     3721 agatatataa agggacttca taccctaata attgataagt tccttcaaac aaaatagaag
     3781 aagatgtcgc ataaaattta taagacattt ttttagtctg ataatcttta agataacttg
     3841 ctacagtttc gttaagctgt tctctggttg tgacaaatgt accaattttg atatttttaa
     3901 ctggtttttt agcaatcagt tctgactcag gttctcttac ttgaatgaga cgacttgcaa
     3961 taaccatcaa aaaggcggta ttaaaagcaa gcagacataa gaagctccac ttccaccaat
     4021 taaggtttga tttttttttc atgcttttta attttctcca ttactgcatt tgacatgatt
     4081 tgatagccag tattatttgg gtgaaaatgg tccccagtaa acaaggcatc attgacaatt
     4141 gtagtttgat ctcctgaaga atggacaatt ccttcttgtc catttatccc tttatacaag
     4201 aggtcattta ttggcacaaa gtacacacga tcgtattctc caacaacctc cttagttttg
     4261 atattccagt catcaatcac tttttgcata tcagttagtt ctggaaaatt caaataaaac
     4321 ggattataga tgcctaaaat aaaaatagga agatctttat tatcttttct ggctaactcg
     4381 ataatctgtc ttaatcgttt ttgatactga cgagctggct ttctaaaact agaaacttgc
     4441 aaatccgcta aattcttccg aataactgcc ataacatcat taccaccaac ggttaacgtc
     4501 attatatctg ctttttttaa agataactgt atctgctttt gttttatcat cctatcaaga
     4561 atttgttgac tggtatcacc agatacgccg taattttgat gattaacatt agccttaaaa
     4621 tattcactaa gatcattcgt taacaaagga acaaaaccac cttgatgagt tagatcacct
     4681 actccttctg tcaatgaatc tcctatagca acatattgga tagctacttg ttcttttttc
     4741 agaaaatcac tctttttcaa acgtgaattt gacttaggga taataatatt taacaataaa
     4801 aaggccagac ataaactaat aacaaagaaa aatatcccac taaataaatg acgattattc
     4861 ataacgaacc ataaccgcaa aagctccttc tcctgtatgt gtttggatga tagagcctgt
     4921 ttctaaaacc gaaatagagt ggttgtaata agctgcgatt ctttctttta aggttaatgc
     4981 caggctagct tcaccagcat aagaaatagc tatctctgca atcgggcgat gactattttt
     5041 agcgaggtaa ctatctaacc atttcgtaaa tgttttatta cctcgacctt tgacaagcgt
     5101 ttttaactca tcatttttaa gtgccataac aactttgaca ttaagtagcg agctcaaaac
     5161 acccgtcaca cgaccaatac gacctccttt tactaaattt tctaaggtag atacaccaat
     5221 gtagagctct gtttttgatt tgatagcttg aacagcagcc aaaatctcat tcaaactagc
     5281 acccgctttt gccatctttg ctgcctcaac gacctgaaat ttcatggctt gatcagtaaa
     5341 tcctgaatct aaaactgtaa caggtgcctc agcgatttca gccccctgac gggaagcttc
     5401 aatagtacct gataaagctg gtgagaggtg aatagcaaca atgtcagtta ctcctttttt
     5461 gaccaaattt tcgtaagttt cagcaaagag accaaccggt ggctgactgg ttttgggaag
     5521 ggatttgcta gccttcataa gactcaaaaa atgaccttct tcttttaaat cattatcaga
     5581 atataatttg ctatcaatca tgacagataa aggcactaca gtaatatcta aagctttaat
     5641 taattctggt tcaatagtta ttgatgaatc tgtaacaatt tttatagttc ccatgtattt
     5701 ttccattcta aactagtttc tcacctcttt attctaacac tccttactaa ataaagcaaa
     5761 actggtagtg ctattgaata atcactcggt agataggtat ggcgagcaat agctgctcga
     5821 agaaaatgcc ttagcaataa cgagatacaa aacgaggcaa aagtcaacct tttcacgctt
     5881 gaacatttgc cccgtctttt acaactacaa taacttatta cctaattatt tatcttcttt
     5941 ggtatcataa tcggcgttaa tcctaatatc attaaaccaa gtaaagctgg ataataagag
     6001 aacccctctc ccgtttgtgg cagggctatt tttctttttg caactgccat acctagcttc
     6061 cgactataag gaactgaaac atcttctttt gaacttccaa tagaagagac taggcgcctt
     6121 aactcctcta taacaacatc tggagtagca ataggaaggt gaaatctctt tttcttagaa
     6181 aaagctgatt ctgcttttct atcttcataa tgagcagcta atacttcact aaagctttca
     6241 cctatagaat agacatgcgt tgtcaaatca aatttttgag ccatcgttaa tgctaacttt
     6301 gtagctgtta cagacaaagc gtccagtttg ttatctaaat gtcctctaat ttcaccagac
     6361 cgcagtgctg tatctgatag gctcctcaaa tgtggtttcg gcaattcagc ctctaaatgg
     6421 ggagcactag aaacagtaag gcgtgtctca acagcatcag ttaaatcaaa tagctcatcg
     6481 tcactaggca ttattacatt ttgctctggc tcaattgtat cttcttcatc ttcttcatct
     6541 gattcatcta attcatttaa taacctatca tcagttaaat tctcaaacat cttatcagtt
     6601 tcattttgag aaataaaaga atcatcatta aaagaatcat catcgagaaa atcttcatag
     6661 tctctgtcag catcatcagt tgtttgagac gaggaccatg ctaacatctc aagttcatct
     6721 ttaggggcca catctttttc aacaagtgtt gagcaatcat caaacttatc tccagttgct
     6781 gagagatgat tggcactaac agtatcattt ccacacgtta aacttgctaa cacaatgatg
     6841 cttgataaac acgctttttt actagtcatt tctatcccct cagcttaaaa ttctcttatc
     6901 tattctacta tttctcagag gtttgtcaag gcaaaccaag ccagattaac agaggaaaaa
     6961 tggtctagca caatgattat tttagtacct aaaaaatcaa tttcatctaa ttatgaaaat
     7021 atcaaatacc aaacataatc ttaatgtttg gcaagtagtt cacgagcctg cgtaagagca
     7081 gcttgtgtca tatctgttcc tgcaatcatg ctagcaattt cttccacacg ttcttcagga
     7141 gttaataacc ttaccttaga aacggtggac tcttctttac tttctttaga aataaaatac
     7201 tgataatcag caatagcaat aacttgaggt aagtgtgaaa tagcgagaac ttgcccatgt
     7261 cgcccaattt tataaatttt ttgggcaatg gcttgcgcaa cgcgaccaga aacacctgta
     7321 tctacttcat caaaaacaat actcgtctta tcttcttttc tagaaatagc agccttgatg
     7381 gctaacataa ggcgtgataa ctctcctcca gaagccacct tgacaagggg cttaaatcct
     7441 tcgcctggat tagtagagat gtaaaattcc aagctttcat taccatcacg attgaatttt
     7501 gatgtggtaa agtgaacttt gaaatctgct ttttccatat aaagttcctt aagttcagct
     7561 ttaatctcag cttctaattg ctctgccaat tgatggcggc gttcactcaa gttagtggct
     7621 gcagcaacca attgtttttc taaggctttc aattctgatt ctagatcccc tgaagacaag
     7681 tcgtctcctg ttaatagctg gtattctttg ataatattat cgtagtagtc aagcacatca
     7741 ttcacattac caccgtattt acgggttaag ctattgagtg tatccaagcg aaattcaatt
     7801 tcctgtaatc gcccaccatc aaaatccagt tggtcaatgg tatctgataa ttgcttgctc
     7861 acttcttcaa gaatataata ggcttcagaa atcgaagtcg acatcccttt gtactctgaa
     7921 tcaaactgct caattgatag taagtcattc atgctagagc gaatattgga tagacttgaa
     7981 aaatcgtcat tatctagcat gacgtaggca ttggtcaggg tatcagcaat ttgtttgtgg
     8041 ttcattaagc gatcacgttc ttgatttaac cggtcatcct cacctcgact caaggcagca
     8101 gcttcaattt ctgctatctg aaaggccaac atatcaatac gatctttgtg ttccttttca
     8161 tttttttgtt tgtcaataac ctggcgacgc aagcttttat aacgatcaaa aataagctga
     8221 tagttctctt tcaattgctc aaaagcctta tcaccaaaag cgtccaaaat ttgctggtgg
     8281 agctgtggtc gcattaattc ttcttggtca tgttgcccat gaatatctac caaaaactgt
     8341 ccgacctgtt ttaaggtggc taggttaacc atctgaccat taatgcggct cacgcttctg
     8401 ccattagcaa agatatctcg gcgaataatc agttcttctt ccatagcaat acctgatgat
     8461 tccagacacg caaccagttc tggggtagca tctaccgaaa agaacccctc aatttctgct
     8521 ttattagctc cacgacgaat cacttctgta ctagcacgcg caccaagcat catattcata
     8581 gcatcaatga taatagactt tcctgctcca gtttcacccg tcaaaaccgt cataccattt
     8641 tcaaaattta gggaaatttc atcaataata gcaaaatttt taatagaaat ttctaataac
     8701 atcactttct cccctatttt tttctaggtt ataccctgca tccaaagcaa aatttcttgg
     8761 cgtatcatat ctgcttctga aggagacttt gcaattaaca ataaactatc atcatcagca
     8821 ataagactaa aaatagcttt tgaaaaatct gctaataaat aacgtttgat taatttacta
     8881 ttgccaggta cgacttttaa atagaggtgt tgttctaaac cttttgtttt gtcagataca
     8941 gctaaaatag tgctctttat cgatctaggt ccttgaacga ttttttttcc actatcttga
     9001 gaaagaccat agatgtaacg tccactgcca gaaggaatct ttactatacc aatttcattc
     9061 atgtctcttg aaatagtagc ctgggttaat tctaacccat gctctgccaa taatcttaag
     9121 aggtcatgtt gcgtctcaat tggatgcgtc aaaaccattt ttttgattaa ttcaagtcgt
     9181 tcactctttt tcatgtttct caaactcctt atgtgccttg gcaacaactt tctgaatcaa
     9241 atgcggggct aacgtttcag gtgtctgtga cttagcaaga tgagctaaaa attcgatatt
     9301 gccatgacca ccctgaatag gggaaaaatc aagtccctta actgtaaatc cgtaacctga
     9361 ggcaaaatcc atgacctttt gaatcacttt ttcatgaatc tgtttgtcct tgacgatgcc
     9421 ttttttacca atctgctcgc gcccagcttc aaattgcggt ttaatgagag caataacctg
     9481 tccctgatct gacaaaacgt tatgcagggc tggcaaaatc aagctgagag aaataaagga
     9541 cacatcaata ctggcaaata caggctgtcc ctcattaaag tcttctggct gagcataacg
     9601 aaagttatac tgttccatac ttcttactcg tggatcttgt ctgagtttcc ataccaactg
     9661 gttagtccca acgtcgacgg catagactag tttggcccca tcttgtaaca taacatcggt
     9721 aaaaccacct gttgaagcgc caatatcaat cccaatttga ttagcaactg atacaccaaa
     9781 aacgtgcagc cccttttcca gttttagtcc accacgactg acgtatttga gtttttcacc
     9841 tttaagtttt aactcagtcc catcgtcaat tttgtcacct ggcttgtcat agcgttggcc
     9901 attaatcaca gagaccacta agcctgccat gacaccacgc ttagcttgct ctctggtctc
     9961 aaataatcct tgcttatagg ctaacacatc tactctttct ttaggcatta agtcgtaacc
    10021 cttctatcag ttttgtaatt atttgaggct taaagcctac atcgctttcg agtgtctgaa
    10081 aaatcgtcaa agcctgatct aaactctctg tcaacagttg gtaggaggct tctaacccca
    10141 ataaacttgg gtaagtagct ttttctgcaa ataaatcctt tttaggcgtt tttccaagat
    10201 cttcaaagct agctgtcacg tctaaaatat catccctaat ctgaaaggcg tgcccgatga
    10261 gcattcccgc ctgctctagt tgttgacgaa cagtaatagc ctgttctgtt acaagagctg
    10321 ctgctttaaa aggaaaagtt aataattttc cggttttatt gaggtgaatc agtgacaact
    10381 gagggagact caaagcttga ttttcccctt tcatatctag catctgacca ccaaccatgc
    10441 caaaagttcc tgaagctagg gaaagttctt ggattaaggc tactttaacc tcgctattta
    10501 attcagcttg agctattaac ccaaaggggt ctaaaaataa gctatctccc gccaaaatag
    10561 cagtagcctc tccaaattgt ttgtgattgg tcagtctacc acgtcggtaa tcatcattat
    10621 ccatggctgg caaatcatcg tgaatcagac ttcctgtatg aatcatttca agggctgcag
    10681 ccaaatcaaa atgagcattc tgtaacgata ccccaaatcc ctcaatcatc tctaataaga
    10741 taagtgggcg aatgcgcttg ccaccactgt caacagaata caagatagca tcaatcaact
    10801 cttcagatac accgttacta gtcgtcttat agtagcgacg aatggcttcg tcaattctag
    10861 ctaatttgtc cattaatcat ccatatctac ttctgtgcca tcagcttgca tcactttgac
    10921 gagtgttttt tctgccgctt gtaaggtctt ttgaagttct tttgagagaa gcattccttt
    10981 ttgaaattct gaaatagctt cttccaaagg gacatcccca ttttcaagtt tgttcacaat
    11041 agtctctaaa tcttgtaaat tttcttcaaa tgttttcgtt tttgacatct tttacctcta
    11101 catctaattg tccatcacgc atcttaatgg ttaactgatc accctttgtt atctgactta
    11161 cagaggctac cagtgcttga tttttttcaa tcattgcata accacgggcg ataattcggc
    11221 tggcatccag tgatagcaaa gcatcctgtg ctttttcaaa gcgagccagc tgactatcat
    11281 attgactagc catattagcc atcagtaaac gcttagcggt agcaacacgg tcttggtatc
    11341 gctcgatttt tgtctgtaat tgactattgg ccaaggaatg gtccaactga accttgtttt
    11401 ctttgacgga actcagacga tctttcatcg tattcatcag ggtcatgcta aggcgatcaa
    11461 ttttttgcag gtaggcatca tataatcgtt ctggttgcct aaaaataaca gattgcgaga
    11521 gtttatccac ccactcttgc cgttgcttaa tccgcctcaa gcaggcctga taggaacgat
    11581 tttgccgctc tactatccaa gacataagat ctgtttttgt aataggcgtt gctaactctg
    11641 ctgctgctgt tggtgtggct gctctgcgat ctgccacaaa atccgctaaa gtcgtgtctg
    11701 tttcatgacc cacactcgaa atcactggaa gttgcgattc aaagatagcc tgaaccacta
    11761 tttcctcgtt gaaggcccaa aggtcttcta tcgagccacc tccacggccg acaatgagca
    11821 agtccaaatc ctctctttga ttggctcttc gaatattagc cactacttct tgggcggctc
    11881 catcaccttg tactttagtt gggaataata aaatctcaac ccctggaaaa cgccgtgaaa
    11941 cggttgtgat aatatctcga atcacagcac cactaggact tgtaatgacc ccaatttttg
    12001 atacaaactg tggcaagggc tgcttgtgtt tttgctcaaa ataaccttct gccgttaatt
    12061 ttttctttaa ttgttcaaac tgcaaagcca aggcacctat accatctggc tctgcctttt
    12121 caatcacgat agagtaagag ccactaggtt cataaagttg gactcgccca attacattaa
    12181 ttttcatgcc ttcttctagg tcgaatccta gttttttata gactcctgcc cacatggtgg
    12241 cttgaatcac agcactttca tcttttaagg aaaaatattg atgagttggt cgttttcgaa
    12301 aattggacac ttgaccagtc agataaaccc gttccaaata agggtcacgg tcaaatttta
    12361 attttaaata tttcgtcaaa tgagtgacgg ttaaataatc tgccatcaag ccacctttca
    12421 cttagttgga cctgacatag tccctttgca tactaatgca attataccat ttttctggat
    12481 aaatagtgag cagaaaaaca aaaaaggaag ccattgctga cttcttatat tctcttatgt
    12541 tgaattgtct aattataact atttatgagt actacggaga gcagattgat aagtttgctc
    12601 tagcaacata gcaatcgtca taggaccgac acctcctggt acaggggtga ttttcgctgc
    12661 aacttctgcc acctcatcaa aggccacatc tccaattagc ttgccattat catcacggtt
    12721 cattcccaca tcaatcacta tcgcaccatc ttttatatat tgttttgtta tgaaatgacc
    12781 ttgtccgatt gccacaatca acacatctgc acagcgacat acttcttcta attgacgtgt
    12841 tcttgaatgt gtcaacgtga ctgttgcatt tttgtccagt aagagctgtg ccattggttt
    12901 tccaacgata ttcgatctgc caataatgac ggcatgttta ccttcaaggt taacattata
    12961 ttctcgaagc aattccataa tccctgatgg agtacaagga accatcaagg gacgtcctga
    13021 ccaaagatga cctgtattca tcgggtgaaa accatccaca tcttttttgg gatcaatggc
    13081 gagaataatt tttttatcat taatatgatt tggcaggggt agctgcacta aaataccatg
    13141 aatagtgtta tctgcattgt aacgttcgat tactgcaata agctcttctt gacaaatgaa
    13201 ttctgataat ctaacggtct cacttttaaa acctacagta agagctgcac gctctttatt
    13261 acggacatac acctgactag caggatcatc acctacaaga ataacggcta agcctggtac
    13321 aattcctttt ttttgtttta gattgttgac tttagctgct aactcttgtt gcatcttttg
    13381 agctaaggct ttaccatcaa ttagttctgt cattgacatc tccctttaat ttcatttttc
    13441 tattataaca aaaaaataga tgcctgttct ttatactctt acttattttt tagtattggt
    13501 ttgagtcgat taaattggtt agtggcacct acaagaaaaa gaaggtgcct aaaagtctca
    13561 cctccttttg tagttgttta tatggaaaaa tagtgtaaaa gtgttatcca tttttgatgt
    13621 ttaattaacc tgatttattt ttgtgcgaca cgctgcttca atagcatcta attttgttgc
    13681 actattttct tgtgtttggc caatagtata taaataaaat tttattttag gctctgttcc
    13741 tgatggtcta agtgcatacc aagaaccatc atccaaataa aatttgagac agttttgctt
    13801 tggaaaatct tgatagccat caataaaatc aattgtcttg tctaacgcca tctctgcaac
    13861 actagctatt ggtgtctgtc tgaaatcttc cattatcctc gcaatgcgtt tttgtccttc
    13921 tatcccctca agctccaagg caatttgacg ttcattataa tacccaaatg tcgcataaat
    13981 agtctgcaaa acatctagta aattctgccc ctttttcttg tagtaagctg ccatttctac
    14041 aatcatcata gaggcgctaa cagcgtcttt gtcacgaaca aaagtgccat agcaaaaacc
    14101 aatactttct tcataaccaa acagatagtt tttctgtttc gtcacatcat attcgtttgc
    14161 cttgccacaa atattcttaa aaccagtcaa tgtttcaacg gtttcaatac cataatggct
    14221 agcaatagcc cgtgacaagt ctcctgttac aatggatttc actaaaacag gattagccgg
    14281 taaattacct aagtcaaatc gttgtgaaaa gatatagtaa gataaaagcg ctccgatttt
    14341 attgccattg agaaaaacat agtcaccgac tgcgttttta acttctaaag ccacacggtc
    14401 acaatctgga tcagttgcaa tcaaaatatc agcatcaact gccttaccta atttttctga
    14461 atatgcaaaa gttttaggga cttcgggatt aggatagcca actgtcgtaa aatcaggatc
    14521 gggcatttcc tgctcaggta ccacataaac attttcaaaa ccgcggcgtc ttaaaacctc
    14581 acgcactggt agattcccaa caccatttaa aggagtatag actactcgga ctgacttatc
    14641 aatgtcagta tcattaatcg ttaaaccaag cacttctttt ttatatgctt cttcaatact
    14701 ctcatcaata taagtaacaa gtccactgtc cagagccttt tcaaaaggca tttgtttgat
    14761 ttcctgatac tgagttagtg cagccatgtg ttgtgcaatt tgatccgcaa tgtcatccaa
    14821 aatttgagaa ccttcttgcc aataagcttt gtaaccattg taagcttggg gattgtgact
    14881 agccgtaatc ataacaccag agatacatcc caaagcacga atagcgtacg aacacattgg
    14941 tgttggccga atccctttat aaaggtacgc cttaataccg ttagctgcca taatagagca
    15001 tgttaattct gcaaatgttc tagattgata gcgaacatca taactaaccg caatgccctt
    15061 tttaactgct tcaggtccat gatcaataat agtattagct aaagcttgtg ctgccttacc
    15121 aaccatatag gtattcatgc gattggtgcc tgctccaagt tttcctctta gccctgctgt
    15181 tccaaattca agtgttttgt aaaaacgatc ttgaatctca gcctcattgt cttttatggc
    15241 tgctaaatct gctttaatat catcactaag atcattattg tgcaaccatt cttgatatac
    15301 ctcgttgtaa gtcatattac tcatcctatt ctaagaccaa atggtaaaaa tcagctccaa
    15361 aacaagaaat cgctttctct aagggagaca actgaatagt atcaaataat ctaaataaag
    15421 tcaacttatt ttttaaatta aataatctta attcatgata actttttgtt taaactctta
    15481 taatgaaaac agtcattcta tttttgcttt ttcttttgcc cactcatact ttgcaatagc
    15541 ttgttcacgt tgtttagcat gatcgacaat tggttgagca tagtctgtac caataataca
    15601 ggacacactc tcttgaaggt tttttggcat cttccacggt tcgtggagat acttctctgg
    15661 aacgtgttct agttgaggca aatacgcttt aataaactcg cctttgggat caaagcgctt
    15721 accttgagta acaggattaa atattctaaa ataaggaaca gcatctgttc cagtggaagc
    15781 agcccactgc catcctccga tattactggc agcatcataa tcaattagtt gttgttgaaa
    15841 atactgctct cctaaacgcc aatcacataa aagatcttta gttaaaaaag aagctactat
    15901 cattcgtaaa cgattatgca tccatcctgt cttttgtaac tgaagcatgg cagcatcaac
    15961 aatagggtaa cccgttttac cttctttcca caactgaaac cagtcaggat tattaaccca
    16021 ttcgatttga gaaaaggctt tttggatagg ctgagttttc tgatcaggat aagccacata
    16081 gaccatattg taaaagtctc tccaagctaa ttcttttaaa aaagtagctt gccctagact
    16141 atttggtgct tgacgcacag cgtgatatac cgtgcgaatg ccaatagctc ctattcttaa
    16201 aaaaggagac aaccgactcg tccctagctg cgctggaaaa tctcgattag cgtgataagc
    16261 tgctaattgg tcttgaataa atcgatttag ttgttttgac gcttcatcaa aagttgcaac
    16321 atcctgatat ttttcatctt taaagctctc aacagtcatt aaaacatcat ctggtgtctc
    16381 aagatttaac cacctgcctt gcgatagttc tactttaata ggagtctctt tcggataatt
    16441 ttgccaaatc ctatagtagg gcgtaaaaac tttataaggc tgacctgatt ggttaatgat
    16501 ctcttgacta ccatgtaaat agtgatcttg gtaggtatga acggcaatcc catttttttt
    16561 gaaaaattgt gctgcttttt gatcacgtaa acgaccatat ccagattcat catagttaaa
    16621 atacacatct gtccagtctt ttaattgtgt caataattgc tcaaaacagg tcattaagtc
    16681 tccatacatt agatataagt caataccttc acattttaat aactttctaa aagccaaaac
    16741 gctagtaata aaactagatt gattccgact atgtagcaga tttaattgct ccttgttaaa
    16801 ttgaaacaca cataaggttg gtgattgact agcaatagca tgagctaaag ctttttgatc
    16861 atcaatcctt aaatctctac gaaaccacat cactgaaacc ataacagatc cctttcttct
    16921 atgcaaataa tgcaactatt tctttttatt ttaacaaaaa gaaaagtaat ctcctataaa
    16981 atgccttagc aaattataaa aggaagtcct aaatcattta ggacttcctt ttataattca
    17041 tagaacagat ttataatact ctactcaaaa aatcttttgt acgctcttct ttagtcagat
    17101 caaaaagttg gttaggactt ccttcttcca caataacgcc gccatccata aaaatcacac
    17161 ggtctgcgac ttctttagca aagcccattt catgagtcac aataaccata gtcatcccag
    17221 atttagccaa atcttgcatg acagccaaga cctcacccac catttctggg tctagagctg
    17281 aagttggttc atcaaagagt aaaacatctg gatccatagc cagtccacgc gcaatcgcaa
    17341 tccgctgctg ttgcccacca gaaagactag caggataagc cttggctttt tctgataatc
    17401 caactttgtc caacaagctc aaggctgttt tgtcagcctc tgctttagcc attcccttag
    17461 ttttgattgg cgataaagtg atattttcta aaatagtcat attgggaaag aggttaaact
    17521 gctggaaaac cattcccatt ttttcacgca tgctgaaaat atcgttcttc ttatcggtaa
    17581 tatcaatccc ctcaaaccta atctgtccct tagttggtac ttccaaaaga ttcatggttc
    17641 gaagtaaggt cgattttcca gaaccagacg ggccaataat caccacaacc tctcctggca
    17701 taatgtctaa atcaattccc ttaagcactt cattgtgccc ataatacttg tgcaagtctt
    17761 taattgaaat aatggcctca gtcatcaatg attacctcct tgcgccatgt gacgttctaa
    17821 gactgccaat aactgtgcca tcactgttgt gactattaag tagtaaaaag cagccaccag
    17881 taaaggggaa attggagaat aagtagccgt taccactgat tgggcaccat tccaaagttc
    17941 catcactcca atggtttgta aaagagcact atccttaata atggtaataa attcatttcc
    18001 taaggctggc aaaatatttt taaaggcctg aggcaaaatc acataacgca tggcattttg
    18061 aggacgaata cctagtgaat aagctgcttc taattgccct tttggtacag cctcaatacc
    18121 tgctctaaca atttctgaaa tataagcacc gctattcaat gaaatgataa taattccagg
    18181 aagtagtctt gaaaagtcca agtctaaaac cccaaaacca atagtaggca tattgttaaa
    18241 atgcatccaa gcaaaggcaa tcatgatttg aaccaccata ggtgttcccc gaaagatcca
    18301 aacgtaaaga ttaacgaccc aggtcaacgg cttcacatga ctacgcttaa tcagggttac
    18361 caagacacca ataagggttc caaaaaagac aacgctgact gaaatcataa tggtgacaag
    18421 tacaccgtag ttaaagtagg cccagtattt gggcaaaaat gacaaatcca tttatatctc
    18481 ctcataagtc ctaggttcat tacaataaag ataattatac catctgatga atattaatac
    18541 aatacttttt gacaataaaa aaacctgcct caggcaagtc tctttactat gtcaagctta
    18601 atataataaa tcgtagattt ccattgcaat caagtcaatg ctatcaaagg aatatgactc
    18661 acgcgcttca acatcacgca atgtgaaaac aggtccattt tcaggatcat ttgaaaaggc
    18721 aacttctgca acaacaacac catcgcgttc aaaatttcgc ttgagattac cgccatctgt
    18781 caccatcaac tccaaacgat tgatgattct caccaaatgt gattccattt cgtttctcct
    18841 attcgttttt tatccctctt attttaacac aaaacaaggg aaactaacaa ggaaagtaat
    18901 atatttattg atattttact ttttttactc ttttcaatga ttttaaacat ttgctcattg
    18961 gaaacttaat aaagttactc tgtcatgtta ctctctaaaa gctaacgcta aagcgataaa
    19021 ctttttactt gatttcccaa gccgtggtgc tataatatga atataaagtt tgaccatttt
    19081 tgactaaatg actttgactt ttagataaac cctcggaggt atattatgct gtgtcaaaat
    19141 tgtaatttaa acgaatctac tattcatctt tatacaagtg ttaatggaaa acaaagacag
    19201 gttgatctct gtcaaaattg ttatcaaatc atgaaatccg atcctgccaa ttctatttta
    19261 aatggcctaa ccccaggata tagagcacaa gatagatcca caagtccttt ctttgatgac
    19321 ttttttggtg atttgaataa ttttagagct tttggtaatc ttccaaatac cccacctact
    19381 caggcagggc aaaatggaaa tggcggagga cgctatggtg gtaactacaa cggacaacga
    19441 cctgctcagc cacaaacacc aaatcagcaa gcaaagggct tattagaaga gtttgggatt
    19501 aatgtcacag atattgcaag aaatggtaat attgatcctg ttattggtcg tgacgaagag
    19561 attacacgcg ttatcgagat cctcaaccgc cgtactaaaa ataatcctgt gctaattggt
    19621 gagcctggag ttggtaaaac tgctgttgta gaaggtttgg ctcaaaaaat tattgatggt
    19681 actgttcctc aaaaactcca aggcaagcaa gtgattcgtc ttgatgtggt tagcctcgtt
    19741 cagggaacag gtatccgtgg tcagtttgaa gagcgcatgc aaaaattaat ggaagaaatt
    19801 cgcaatcgca aggatgtgat tctctttatt gatgaaattc atgagattgt cggtgctggt
    19861 tctgcaggag acggcaatat ggatgctggt aatattttaa aaccagcctt ggcccgtggt
    19921 gagttgcaac tcgttggtgc tactacatta aatgaatacc gtattattga aaaagacgct
    19981 gccttagaac gacgtatgca acccgttaaa gttgatgaac cttctgttga ggaaaccatc
    20041 acgattttaa aaggtatcca accgaaatac gaagactatc atcatgtcaa atatagccca
    20101 gccgctattg aagctgccgc tcatttatct aaccgctata ttcaagaccg ttttcttcct
    20161 gataaggcta ttgatcttct ggacgaagct ggttccaaaa tgaatctaac cctcaacttt
    20221 gttgatccta aagaaattga caaacgtctt attgaagctg agaatctcaa ggcgcaagct
    20281 actcgagacg aagactatga acgcgcagct tatttccgcg atcaaattac aaaatacaaa
    20341 gaaatgcagg ctcaaaaagt cgatgagcaa gatattccca tcattactga aaaaaccatt
    20401 gaagctattg tagagcaaaa aactaatatt ccagttgggg acttaaaaga aaaggaacag
    20461 tctcaactcg taaacttagc caatgatctg aaagcacacg tgatcgggca agacgatgct
    20521 gttgataaaa tcgctaaggc tattcgtcgt aaccgtgtgg gattaggaac tccaaatcgc
    20581 cctattggtt ctttcttatt cgttggaccg actggggttg gtaaaactga attgtctaaa
    20641 caactagcca ttgaactctt tggttcgaca aacaatatga ttcgctttga catgtccgaa
    20701 tacatggaaa aacacgctgt cgccaaatta gtcggggctc ctccaggtta tgtcggctat
    20761 gaagaagctg gacagctaac cgaacaagtt cgtcgcaatc catattcact tattctctta
    20821 gatgaggtgg aaaaagccca tcctgacgtc atgcacatgt tcttacaagt tct
//
