LOCUS       NZ_CRAW01000001        10300 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus pneumoniae strain SMRU702, whole genome shotgun
            sequence.
ACCESSION   NZ_CRAW01000001
VERSION     NZ_CRAW01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  AUTHORS   Informatics,Pathogen.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2015) SC, Wellcome Trust Sanger Institute, CB10
            1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001193635.1-RS_2024_12_12
            Annotation Date                   :: 12/12/2024 01:34:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,158
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 44
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 66 of 177
            Pseudo Genes (incomplete)         :: 114 of 177
            Pseudo Genes (internal stop)      :: 46 of 177
            Pseudo Genes (multiple problems)  :: 43 of 177
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:02:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 82140
            Orig. end                         :: 92440
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CRAW01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10300
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10300
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10300
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5300](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5300)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    406..420
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 16.66"
                     /tool="antismash"
     gene            complement(810..2162)
                     /gene="murD"
                     /locus_tag="AK725_RS00460"
                     /old_locus_tag="ERS020212_00094"
     CDS             complement(810..2162)
                     /EC_number="6.3.2.9"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /gene="murD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /locus_tag="AK725_RS00460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS020212_00094"
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="WP_000863027.1"
                     /transl_table=11
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFED
                     NPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTEVE
                     LAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATDKNT
                     LVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAADFLVL
                     NFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNVE
                     NALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQKA
                     LSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAYVEA
                     TDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE"
     gene            complement(2266..2907)
                     /locus_tag="AK725_RS00465"
                     /old_locus_tag="ERS020212_00095"
     CDS             complement(2266..2907)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /locus_tag="AK725_RS00465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS020212_00095"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_050265533.1"
                     /transl_table=11
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLLN
                     MIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLVGK
                     KLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILADEPT
                     ASLDSKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(2909..4930)
                     /locus_tag="AK725_RS00470"
                     /old_locus_tag="ERS020212_00096"
     CDS             complement(2909..4930)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /locus_tag="AK725_RS00470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS020212_00096"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_050265534.1"
                     /transl_table=11
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKDT
                     QEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQES
                     RERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILISLA
                     IFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFLGGGV
                     VLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGRLPLKR
                     LMALTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGLGDRGKD
                     TENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANTLYVSPSY
                     LDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYGKSGEEASA
                     PLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDSFISLSSWSI
                     NAGKQLFIKGYESGIKLLKNAGIYEQVSYLKEGRSVYLTRYNEVQTETATLILGAIVGI
                     ASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFVFGASLFILSSR
                     DLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(5001..5300)
                     /locus_tag="AK725_RS00475"
                     /old_locus_tag="ERS020212_00097"
     CDS             complement(5001..5300)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /locus_tag="AK725_RS00475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS020212_00097"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000850389.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.1e-26,
                     bitscore: 86.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGGW
                     AFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     misc_feature    complement(5488..5501)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.53"
                     /tool="antismash"
     gene            complement(5666..5920)
                     /locus_tag="AK725_RS00480"
                     /old_locus_tag="ERS020212_00098"
     CDS             complement(5666..5920)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /locus_tag="AK725_RS00480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS020212_00098"
                     /product="DUF3165 family protein"
                     /protein_id="WP_000262800.1"
                     /transl_table=11
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVLS
                     FLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(5940..7781)
                     /gene="typA"
                     /locus_tag="AK725_RS00485"
                     /old_locus_tag="ERS020212_00099"
     CDS             complement(5940..7781)
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /GO_process="GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="typA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /locus_tag="AK725_RS00485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS020212_00099"
                     /product="translational GTPase TypA"
                     /protein_id="WP_000164111.1"
                     /transl_table=11
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAERA
                     MDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVVDA
                     YEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQLDF
                     PVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLDYNDF
                     VGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAGDLIAV
                     SGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSRKVEERL
                     QAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEVIVKEIDG
                     VKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPARGLIGYST
                     EFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMSIEERGTIFV
                     NPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPRILTLEESLEF
                     LNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            7943..8668
                     /locus_tag="AK725_RS00490"
                     /old_locus_tag="ERS020212_00100"
     CDS             7943..8668
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1154: Pseudouridine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /locus_tag="AK725_RS00490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS020212_00100"
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="WP_001235020.1"
                     /transl_table=11
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGLQ
                     ELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLDRD
                     TTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQTFQKGIVFLDGTICKPA
                     RLEILSASPSFSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLAESNY
                     RPLNQKELQIIKNYLEMSR"
     gene            complement(8708..8935)
                     /locus_tag="AK725_RS00495"
     CDS             complement(8708..8935)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /locus_tag="AK725_RS00495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000660078.1"
                     /transl_table=11
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKESQ
                     LDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(9024..9404)
                     /locus_tag="AK725_RS00500"
                     /old_locus_tag="ERS020212_00101"
     CDS             complement(9024..9404)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /locus_tag="AK725_RS00500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS020212_00101"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_000259256.1"
                     /transl_table=11
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQL
                     IDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLKKQ
                     GFSEIYILSYGLDSWKGKVKTS"
     gene            complement(9404..9673)
                     /locus_tag="AK725_RS00505"
                     /old_locus_tag="ERS020212_00102"
     CDS             complement(9404..9673)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /locus_tag="AK725_RS00505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ERS020212_00102"
                     /product="YqgQ family protein"
                     /protein_id="WP_000051787.1"
                     /transl_table=11
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIEL
                     MKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
ORIGIN
        1 ctatcgccaa gagctcaaaa atcgtattgg caccaccacg tgtcacaaca atatcagcca
       61 attccatcaa gggttgatag agatcggtca catagtcaac acgaaaaaga ttttggctca
      121 actcattcag gctagaatct ccagttagat tgataatatt gtagcgctct gttagttctt
      181 tcttatggtc tgtcaccaat tggttaaaga cacgagcacc tgcagaacca ccaacaaaca
      241 atacagtcgg caatttatga ttaaagtggc tttgaatatc caccaattca tctggttctg
      301 gatttttttg atctgaaacc ttggtcactg ctcccacatg ctcaacctta gacaaactcg
      361 aagcttgctc aaaggttgaa tacatcttag tcgcaaactt ataggcgatt ttattggcca
      421 agcccataga caggtcagat tcgtgaataa agacaggcac tcctgacaca cgcgcagcga
      481 taacaggcgg tactgagaca aagcccccct ttgaaaaaag ggtctgtgga cgaagtcgca
      541 acatgataaa gagcgattgg acgattcccc agccaacttt gaagacgtcc agcatatttt
      601 gccaagagaa atagcgacgc aattttccag tcgcaataga atggaaggtg acatccaaac
      661 ctgacttaag gatttcttgg tgttcaatac cacgcttgtc cccgatatag tggacttccc
      721 aaccatcttc gatgaacttg ggtattaaca aaagattgag ggtaacgtgt ccaaccgtcc
      781 ccccacctgt aaagacaatt tttttcatat tattctttta actccgctac tgtgtcgata
      841 aagaggtcgc cacgtacttc aaagttagca tacatatccc agctggcatt ggcaggacta
      901 agaagaacca catctccttg agtcgcaagc tcataggcct tgcgggtcgc atctgcaata
      961 tctgtcgcct ccacataagc gacaccagcc ttgtctgctg cccgtttgac acgttctgca
     1021 gattgaccca ggatgaccat cttcttgagt ccagtaatgt ctggcaccaa ttcgtcaaac
     1081 tcattgccac ggtccaaacc acctgcaatc aagacgacct tgctgttgtc aaatcctgat
     1141 aaggcttttt gagtagccaa gatattagtt gatttactgt cgttatagaa tttaacaccc
     1201 ttgatgtcat ccacaaactg gagacggtgt ttgacaccac cgaaggctga aagagtttcc
     1261 ttgatggttt gattgtccac atcacgaagc ttggctacag caatagtcgc aagggcattt
     1321 tccacattgt ggctacctgg aacaccgatt tcattcgctg ccatgactac ttcaccacgg
     1381 aagtagagtt gaccatcttc cagataagct ccatcaacct tttcaagtgt tgaaaatggt
     1441 acaacagtgg cttctgtctt ggaagccaag tcttttgcca agtcttgatt aaagttcaag
     1501 acaaggaaat cagctgctgt catcttgttc tggatattcc acttggctgc tacatattcc
     1561 tcaaataatc catgataatc gatatgagtt ggcataaggt tagtaataac cgcaatctca
     1621 ggatggaatt cttgaacacc catgagttgg aaagaagaaa gttccataac aagcgtgttc
     1681 ttgtctgtcg ctatttgagc aacctgactg gcaggatagc cgatattccc tgataaaaga
     1741 ccatgttggc cagcagcagt caaaacttcc ccaatcatag tcgttgtggt tgtcttaccg
     1801 ttcgatcctg tgataccaat aatcggtgct tctgaaatca aataagccaa ttccacctca
     1861 gtcaagactg gaattccctt ggccaaagcc ttttcaatca tgggattgct gtaggggata
     1921 cctggatttt ttaccatgag ggcgaactct tcatccaaga gttccaaagg atggccacct
     1981 gtaatgacct tgatcccttc ttccagcaaa cattgcgcag ctggattgtc ctcgaatggt
     2041 ttcccatcat ttactgtcac aatggcacct aacttgtcca acaaacgagc cgcagattca
     2101 ccagacttgg ccaaacctaa aacaagaact ttcttatttt taaattgatc tattactttc
     2161 atgtctcgaa ctccatttct actcctacta ttttaccatt tttatggaaa taaaaaagcc
     2221 acaaagtgtg tttgtgactc tttcttctaa ctgaatctta ccatatcatc tatgtgataa
     2281 atcggtaact cgaatgacct gatccacttg ctcccaaatc agaggattgt gggtcgcaat
     2341 aataatggtc cgattcggat tttttaaaga ttctaggatg gaaagtaatt cctcagagtt
     2401 tttggagtct agtgaagcgg ttggttcatc tgcgaggatc aaaggtggat cctttaaaat
     2461 tatcttcgct agtgcaacac gttgtgcttc tcctcctgat aactcaaata taggttgctt
     2521 caaatccaaa taagagaggt ttacacggtt tagagcttgt ttcatcaaag agattttctc
     2581 tttttccttc aactttttac caactaaacc cagattgaga ttctctttga cggtttggct
     2641 ttcaattaag ccaaaatctt gaaataagta tcccaagtaa tctctaaaga aaacagaagg
     2701 cttgatgtcc ttaagagaag tgccatcata gatgatttgc cctttgtcat atggctctaa
     2761 tcgtccaatc atattcaaga gtgttgtctt accacagcca cttgtaccga ttaaggcata
     2821 aattttccca ccttcaaaat gaagattcgt atctgaaaat agctgacggc ttccaaattt
     2881 tttagatata ttctttagtt caatcatcct attttccttt cataattgtc atagaaacac
     2941 gagattcttt ctgcgcttga cggtaaagcg tcaaaactgc actagctaga aagaccaata
     3001 aagtgagcaa gccaatcacc aagtctcgac tgcttaaaat aaagagacta gcaccaaata
     3061 caaaactggc aaattggcta accatatact gagcatgtgt ttcaaaaaat cgtaaacctg
     3121 aaattcgttt aatcaagata tctcggcgga attgctcgaa atatagaaga ttgacagaat
     3181 aaaagagtaa caaggaactg gctattccaa caatagctcc taggattaaa gttgctgttt
     3241 cagtttgaac ttcattataa cgagttagat aaacacttct tccttcttta agataggata
     3301 cttgctcata aattcctgca tttttcaaga gtttaatccc actctcatat cctttgataa
     3361 agagttgttt tccagcattg atagaccaac tagataagga tataaaacta tcacctgtag
     3421 aagtcggcgt gaataccact aaaatcggat cagtcaaata ctgagtagat acgggattct
     3481 caccgttatt ataaacaaac cgcttttctc ccattgaaag ataactaacg tgcgctttca
     3541 tctcataatc caaaggagca cttgcctcct caccagattt tccataataa ctcaatcttt
     3601 cttcaaaaac tttcttaagt tctgcttctc gagagcgcaa atgttctggg agcaagagga
     3661 taaactcacc tttttggaga tgggctaact tctgtttggt ctcagcatct accacgacct
     3721 tttccttgtc caaataactg ggactaacat agagcgtatt agcatctgga ctataggtat
     3781 ccagtgtctc tccctgttca ttttttcctt gtggattggc aaaatggagc agattatcct
     3841 ttacataaag agcttgttct tcttcgattg cttccttggc aaaggcatac cacttgctct
     3901 gattttctgt atcttttcct ctatcaccta agccaaagga aatctggtaa tagtctgctc
     3961 tatcctgcca tgcttgtttt gaaatttcaa gttctttcaa tcgttggtaa gacgtcaaac
     4021 ctgtcttaac agcgtagcct actgtaaaaa cagctactaa ctgacacaat agggttaaag
     4081 ccatcaagcg tttaaggggt aatcttccct taataacggg aactaatgct ttgtaactca
     4141 aactcattag gtaaaggagc attagtaaaa ttgaaatcgc caataaaaac aacagataga
     4201 aactaatccc aaaaccatag gtggctaaca agataggata aaacaaacct tgactaaaaa
     4261 gaacgactcc cccacctagg aaggaaagga gggctgatag aaggagccat ttgatatcag
     4321 tagataaaga atgccccatg atggataaga gagtctgacc agaaaagagt tttatacctg
     4381 ctgctctcat ttccttaatc cgagtgataa tcactaaagc aaagaaagat aagccaaata
     4441 ttgctaaact aattaaaata aggggattta gtaatattcg aaaagcaaga aaatagggcg
     4501 gtatctttcg gtcagcactt gctttataac ccaaatctcc taatttatcg gcaagctttt
     4561 ctttcgtcaa ggagcctgac aaaaggagat aactatttag cggatcacta cgttcacgac
     4621 tttcttggct agcttcttgg aattcttttg gtaaagttcc ctgaccataa gttgcataag
     4681 taaagtgagt cgtcccatcc ttactcggct ctacaattct tctagctatt aaactctgtt
     4741 ctgagtttgc aaaattctcc aattcctgtt caaatacctc acgcgtcggt tcctgagtat
     4801 cttttttgac acgaagtaaa gaaacggaat catagcttgc atataaatat tgtggcgcac
     4861 gtaagacaat aatccaagca aggaaaaagc tgagaaaaaa agttgataat aatatgaata
     4921 gtttcttcat agtagactcc ttgtaaacaa aattccccct gtaatttctt acaaggggaa
     4981 cgatttaaat caatgaacga ttagtcataa tcacagtaaa atgctacttg ttctccccat
     5041 ttagtccaaa tccatgcata ggaggttttt ccagcagaag cttctccttt atctgaatga
     5101 ctattccatc tacttacaag agaagaataa tgatattttt taccatgata gtaatttgaa
     5161 aaagcccaac cacctcctga accttctcca tatgtccata ctcctccatc tggatattgt
     5221 acagcagctg atgcagctcc caataatgta aaacttgaaa taagagctag agcaagtaat
     5281 ctatgttttt tcgttttcat tttatttttt ctttcaaaaa aagcacacct tgagcaacaa
     5341 tgcaacaaaa taaaccctcc tctctctttt gttgaaaccg ctttcttatg tgataaaaat
     5401 atcattttta ttatttgttg tcaaggaaaa aatcgaattt tttagatatt ttactatatt
     5461 acctctatga ataatattat atagtagttt tattttaaaa taatatgcaa ccagtactaa
     5521 ccaaatataa aatagatgcc attaacgaat tttattcaag tttttcccat tcatactatc
     5581 caagcaaaag agatgatgtt atagtacaaa acaattcaaa ctattgtaaa attcctagca
     5641 aaaaagagag ccaaaactct cttttttatc ttcttttact ttttttgact ggcatgagtg
     5701 taatgtctct aacactaaag taagctagga tcaacatggc tattgctagg aatatttctg
     5761 ttggtaattg aaaaattttc agaaaagata gaaccaataa aatcaagagt gccactaaaa
     5821 tacataccat agcgacgata ttgactgtcc ctttaatgct ttctggtgtc gcaaatacat
     5881 agagtaggag cagtaaaatt cctaggacta aatagaccat ctttctctct ttctagctct
     5941 tattcagctg attttttctt cttgttagct ttctcacgct ctgctttgtt aaggatttgt
     6001 ttacgcaaac ggatagactc aggcgttact tccatgtact catcgtcgtt caagaactca
     6061 agagactctt caagtgtcaa gatacgaggc gtcttgataa cagctgtttg gtccttagta
     6121 gctgaacgaa cgttggtcat ttgttttgcc ttcgtgatgt taactgtcaa gtcattttca
     6181 cgagagtttt caccgatgat cattccttca taaacctcag tacctgggtt gacaaagatc
     6241 gtaccacgtt cttcgataga catgattgag taagttgtag ccttaccagc atcgatagaa
     6301 acaagggcac cacggtgacg tccaccaatt tcccctggaa tcaatggcaa gtattggtcg
     6361 aaggtatggt tcatgatacc gtaaccacga gtcattgaca agaactcagt tgagtatcca
     6421 atcaaaccac gcgctggaac aaggaagacc aaacgagttt gaccattacc agttgaaatc
     6481 atatccaaca tttcaccttt acgttcagaa aggctttgga taacagaccc ttggtattct
     6541 tctggagtgt cgatttgtac acgttcaaat ggttcacatt taacaccgtc gatttctttt
     6601 acgataactt ctggacgaga tacttgaagt tcatagccct cacgacgcat tgtttcgata
     6661 aggattgaca agtgcaattc tccacgtcct gaaacagtcc atttatctgg tgaatcagtt
     6721 gggtcaacac gaagggaaac gtctgtttgc aattctgcct gcaagcgttc ttccacctta
     6781 cgagaagtta cccatttacc ttctttacca gcaaatggtg agttgttgac caagaaagtc
     6841 atttgaagag ttggctcatc gatgtgtagg attggaagag cttctactgc atctgtcgga
     6901 gtgatggttt caccgacaaa gatgtcttcc atacctgaaa cggcaatcaa gtcacccgct
     6961 ttggcttctt ggatttcacg acgttccaaa ccaaagaaac cgaagagttt tgtaacacgg
     7021 aagtttttag ttgtaccgtc aagtttagaa agggtaactt ggtccccaac cttaactgta
     7081 ccacggaaga cacgaccgat accgatacgt ccaacgaagt cattgtagtc caaaagcgac
     7141 acttggaact gcaaaggctc atctgagtta tctactggag ctgggatatg gtcgataatc
     7201 gtgtcaaaga ttggtgccat agtcgcttct tggtcagctg gatcatctga caatgaagaa
     7261 gttccgttga tcgctgaagc ataaaccact gggaaatcaa gctggtcgtc atctgcacca
     7321 agctcgatga aaagttccaa gacttcatcc actacttctg ctggacgagc tgatggctta
     7381 tcgattttgt taacaaccac gattgggaca aggtcttgtt ccaaggcttt tttcaatacg
     7441 aaacgagttt gtggcatggt tccttcatag gcatctacga ccaagacaac accgtcaacc
     7501 attttcatga tacgctcaac ttctccacca aagtccgcgt gtcctggtgt gtccataatg
     7561 ttgatacgag ttccgttgta agcaacggca gtatttttag caaggatggt aatcccacgc
     7621 tctttttcga tatcgtttga gtccatagca cgctctgcca attcagtacg tgcatcaagc
     7681 gtttctgatt gtttcaataa ttcgtcaacc agggttgttt taccgtggtc aacgtgggcg
     7741 ataatcgcaa tgttacggat atcttctctt aattttgtca tgatttcctc tataatattc
     7801 aaaatttatt ttctaactga acgattatac cataatttca agtaaataac ataactcaag
     7861 caagtgtaaa tgttttcact ctgcttttct tttcacgtca agccttttca aagcgagcga
     7921 cttatgataa gataggcaca gtatgcgttt agataattta ttagcccaag aaaaaatcag
     7981 ccgaaaggcc atgaagcaag cactcctcag aggggaaatt ctagtcgatg gttgcccagc
     8041 ccgctcccta gctcaaaata tcgatacagg actacaagaa ctcctttttc aggatcgaat
     8101 cattcaaggc tatgaacaca cctatcttat gcttcataaa cctgctggtg tcgttacagc
     8161 caacaaagac aaggaacttc cgaccgtcat ggacctgctt ccatctgaca tccagtctga
     8221 caagctctat gccgtcggcc gactggaccg agatacaaca ggactccttc tcttgaccga
     8281 taacggtccc ttgggcttcc aactcctcca tccccaatat catgtcgata agacttacca
     8341 agtccaggtt aatggacttc taacacctga ccatatccaa acctttcaaa aaggaattgt
     8401 ctttttagat ggcactatct gtaaacctgc aagactagag attctatcag caagtccttc
     8461 cttcagtcaa gcctctatca ccatttcaga aggaaaattt catcaaatca agaaaatgtt
     8521 cctctcggtt ggtgttaagg tgactagcct caagagaatc caatttgggg acttcacatt
     8581 gaacccagat ttagcagaaa gtaactaccg ccctttgaac caaaaagagt tacaaatcat
     8641 taaaaactat ttagagatga gtcgataaaa caaaaaaagc tttaaaacta aagctttttt
     8701 cttttattta ccgaaaaatt aaggcgattg ctacaatcca gttaactaca gaaatcacaa
     8761 ttcctaagat attaagaatc ttttctattt tatagtctaa ttgtgactct ttttggtatg
     8821 aaatagccaa gaccaatcct atgataccca aaatcaggcc tacaattgga aataacaaac
     8881 caagaataat cgacaagata cccacaaaaa gtggattttt cttcttttct tttatgttct
     8941 aagaactcct taaattttat acaaattaat tatactataa aacaatagct tcatcctatc
     9001 attcgactaa tttggaaata aggttagcta gtcttcactt tccctttcca agaatccaag
     9061 ccataagaaa ggatataaat ctcagaaaaa ccttgttttt tcaagtaaag agctgcattt
     9121 gtaactcgtt gcgcacgttg gttttcgtag agaaggacag gtttatcttt acgaagggct
     9181 gcaagactag ttttcaactg acttgaagga atattgcgtg caccaaggat atgttttctg
     9241 tggaattctg ctgggtcgcg caaatcaatc aattgacccg tacgaatcaa ggcttcaaac
     9301 tcctcattgt ccacaatttt agccgcacgg cgaatacgaa gatagttaaa gcccatccac
     9361 gccaacattg ctagtataag tgcccacaaa atccaagtaa ccattagttc ttttctccat
     9421 ttttctcaat ataatccaat tctaccttgt gctctctgcg aagaactgct tctgcctcta
     9481 gatagtctaa tttatccatc aaccctgcat cgtaaatccg agatagttcc aacttcatca
     9541 gttcaatatc atataagcgt tttcccatgt aaacaataat accaaatcgt ttgaggaatt
     9601 gctgcacatc atagaatgtt ttcataagac tcattctagc aaaattttgt gtttttttca
     9661 agaagagact cacacaatgc tccttatttt cctatcttct ttagcgattc taaggcaagt
     9721 atggtacaat aaaaacatga gaattcaaca attacattat attatcaaaa tcgtcgaaac
     9781 tggctccatg aatgaggcag ccaagcaact ctttatcact cagccaagtc tctccaatgc
     9841 agtgcgagat ttggaaaatg aaatgggcat tgagatcttt atccgcaatc ccaagggaat
     9901 caccttgacc cgtgatggga tggagtttct ctcttatgcc cgtcaggttg tcgagcagac
     9961 ccagcttctg gaggaacgct ataaaaatcc tgtcgcccac cgcgaactct ttagcgtttc
    10021 gtctcaacac tatgcctttg tggtcaatgc ctttgtctct ttgctcaaga aaagcgatat
    10081 ggagaaatac gaactcttcc ttcgtgaaac tcggacttgg gagattatcg acgacgtcaa
    10141 gaacttccgc agtgaggtcg gggtcctctt cttaaacagt tacaaccgtg atgttttaac
    10201 caagatgctg gatgacaatc acctgctagc ccaccatctc ttcacagcgc aaccgcatat
    10261 ctttgtcagc aagaccaacc ctctggcaaa gaaagacaag
//
